CN115447817A

CN115447817A - Full-automatic nucleic acid magnetic bead extraction tube filling and film sealing equipment and method

Info

Publication number: CN115447817A
Application number: CN202211129980.6A
Authority: CN
Inventors: 王世德; 刘跃; 顾柳锋; 许涛涛; 蔡向华; 陈丽娜; 陈芝娟; 贺贤汉
Original assignee: Anhui Bori Biotechnology Co ltd
Current assignee: Anhui Bori Biotechnology Co ltd
Priority date: 2022-09-16
Filing date: 2022-09-16
Publication date: 2022-12-09
Anticipated expiration: 2042-09-16
Also published as: CN115447817B

Abstract

The invention discloses a full-automatic nucleic acid magnetic bead extraction tube filling and film sealing device and method. The filling and film sealing equipment comprises a rack, a station switching mechanism, a feeding mechanism, a flattening and material cleaning mechanism, a magnetic bead filling mechanism, a liquid injection mechanism, a film sealing hot-pressing mechanism, a cold-pressing mechanism, a film cutting mechanism and a discharging mechanism, wherein the feeding mechanism corresponds to eight stations on the station switching mechanism in position respectively. The eight stations are respectively a feeding station, a flattening and cleaning station, a magnetic bead filling station, a liquid injection station, a hot pressing station, a cold pressing station, a film cutting station and a discharging station. The station switching mechanism comprises a turntable, an extraction tube jig and a driving mechanism. The rotation of carousel drives every extraction tube tool all in proper order through eight stations. The extraction tube jig is provided with a plurality of placing positions; the bottoms of the placing positions are all arranged in a hollow way. The full-automatic filling, film sealing, cutting and blanking process of the nucleic acid magnetic bead extraction tube can be completed fully automatically, automatic production is realized, manpower is reduced, and production efficiency is improved.

Description

Full-automatic nucleic acid magnetic bead extraction tube filling and film sealing equipment and method

Technical Field

The invention belongs to the technical field of reagent filling and membrane sealing, and particularly relates to full-automatic nucleic acid magnetic bead extraction tube filling and membrane sealing equipment and method.

Background

In the prior art, the six-connecting-tube filling and sealing membrane for extracting nucleic acid magnetic beads is mostly finished by using full-manual and semi-automatic membrane sealing equipment; the specific process is that the six-connecting pipe is manually placed into a tool, then a reagent is filled into the nucleic acid magnetic bead extraction pipe by using a pipette gun, then the tool is placed into semi-automatic membrane sealing equipment to complete membrane sealing, finally, the nucleic acid magnetic bead extraction pipe with the membrane sealed is manually taken out of the tool, and each nucleic acid magnetic bead extraction pipe is separated by using scissors. This operation requires a large amount of labor cost and is inefficient.

Most of the existing full-automatic equipment filling and film sealing equipment aims at deep pore plates (such as 96 pore plates) with a large number of pores; the six-connected-tube film sealing device is characterized in that the six-connected-tube film sealing device is arranged on the upper end of the six-connected-tube film sealing device, and the six-connected-tube film sealing device is arranged on the lower end of the six-connected-tube film sealing device.

Disclosure of Invention

The invention aims to provide a full-automatic nucleic acid magnetic bead extraction tube filling and membrane sealing device and method.

The utility model provides a full-automatic nucleic acid magnetic bead extraction tube filling membrane equipment, includes frame, station switching mechanism to and with eight stations on the station switching mechanism respectively the feed mechanism that the position corresponds, the clear material mechanism that flattens, magnetic bead filling mechanism, annotate liquid mechanism, seal membrane hot pressing mechanism, cold pressing mechanism, cut membrane mechanism and unloading mechanism. The eight stations are respectively a feeding station, a flattening and material cleaning station, a magnetic bead filling station, a liquid injection station, a hot pressing station, a cold pressing station, a film cutting station and a discharging station.

The station switching mechanism comprises a turntable, an extraction tube jig and a driving mechanism. The horizontally arranged rotary table is rotationally connected to the rack and is driven by the driving mechanism to rotate. Eight extracting tube jigs are arranged on the turntable; the rotation of carousel drives every extraction tube tool and all passes through eight stations in proper order. The extraction tube jig is provided with a plurality of placing positions; each placing position can be used for placing a nucleic acid magnetic bead extraction tube. The bottoms of the placing positions are all hollowed out.

The feeding mechanism is used for placing a nucleic acid magnetic bead extraction tube on the extraction tube jig of the feeding station.

The flattening and material clearing mechanism comprises a flattening component and a material clearing component. The flattening component is used for pushing and pressing the extracting tube jig on the flattening material cleaning station downwards, so that each nucleic acid magnetic bead extracting tube is placed on the extracting tube jig to be flat. The material cleaning assembly is used for pushing the nucleic acid magnetic bead extraction tube out of the extraction tube jig in a mode that the material cleaning inserting plate is upwards inserted into the placing position of the extraction tube jig.

And the magnetic bead filling mechanism is used for adding magnetic beads into the nucleic acid magnetic bead extraction tube. The liquid injection mechanism is used for adding a reagent into the nucleic acid magnetic bead extraction tube.

The film sealing hot-pressing mechanism is used for sticking the aluminum film on each nucleic acid magnetic bead extraction tube of the hot-pressing station in a hot-pressing mode.

The cold press mechanism is used for extruding and cooling the aluminum film on the cold press station.

The membrane cutting mechanism is used for cutting the aluminum membranes positioned on the membrane cutting station, so that the aluminum membranes on the nucleic acid magnetic bead extraction tubes are mutually independent.

The blanking mechanism is used for taking out each nucleic acid magnetic bead extraction tube in the extraction tube jig of the blanking station.

Preferably, the extraction tube jig comprises a jig base and a jig lifting table. The jig lifting table is connected to the jig base in a sliding mode. The tool base includes extraction tube base, cuts membrane deflector and tool guiding axle. The base of the extraction tube is fixed on the turntable. A plurality of vertically arranged jig guide shafts are fixed at the top of the extraction tube base; the film cutting guide plate is fixed with the top end of each jig guide shaft. N through grooves for placing are sequentially arranged on the film cutting guide plate at equal intervals. n is the number of placing positions on the extracting tube jig. Cutter groove of stepping down has all been seted up between two arbitrary logical grooves of placing. The extraction tube placing box is connected to the jig guide shaft in a sliding manner; the placing position is arranged on the extracting tube placing box. The n placing through grooves are aligned with the n placing positions respectively.

Preferably, the feeding mechanism comprises a front integration component and an extraction pipe transfer component. The front integrated component has two. The two preposed integration components are used for arranging the stacked nucleic acid magnetic bead extraction tubes into an n extraction tube array; n is the number of placing positions on the extracting tube jig. The extraction tube transfer assembly is used for conveying the extraction tube arrays arranged by the two front integration assemblies to an extraction tube jig of the feeding station.

The front integration component comprises a vibrating disk, a grabbing component and a transverse moving alternate component. The vibrating tray is arranged on the frame and used for arranging and outputting the stacked nucleic acid magnetic bead extraction tubes one by one. The grabbing component is used for grabbing the nucleic acid magnetic bead extraction tubes one by one onto a storage platform of the transverse moving alternating component.

The cross sliding alternate exchange assembly comprises a cross sliding base, a cam groove, a synchronous belt, a first roller, a lifting frame, an alternate guide shaft, a first storage table, a sliding plate, a linear guide rail, a second storage table and a synchronous wheel. The transverse moving base is fixed on the frame. A lifting guide plate is fixed on the transverse moving base; the lifting guide plate is provided with a cam groove. Four synchronizing wheels which are arranged in a rectangular shape in a vertical plane are rotatably connected to the lifting guide plate. The four synchronous wheels are connected through synchronous belt transmission. One of the synchronizing wheels is driven by a motor. The second storage platform and the sliding plate are connected to the transverse moving base in a sliding mode through the sliding block. The sliding plate is lower than the second storage platform.

The lifting frame and the sliding plate form a sliding pair which slides along the vertical direction. The bottom of the lifting frame is rotatably connected with a first roller. The first roller is disposed in the cam groove. The top of crane is fixed with first storage platform. The first platform of depositing and the second are deposited and all are provided with n and deposit the position on the platform. The relative position of each storage position corresponds to the relative position of each placing position on the extracting tube jig respectively.

The cam groove is in a shape with two high ends and a low middle. When the first roller is positioned at the highest position of the end part of the cam groove, the first storage table and the second storage table are equal in height. When the first roller is at the lowest position in the middle of the cam groove, the top of the first storage table is lower than the bottom of the second storage table, and the first storage table can pass right below the second storage table. The sliding plate, the second storage table and the two horizontal sections of the synchronous belt are fixed respectively. When the synchronous belt moves, the sliding plate and the second storage table move reversely at a constant speed to drive the first storage table and the second storage table to exchange positions.

The extraction tube transfer assembly comprises an extraction tube moving module and a feeding clamping jaw group. And the tail end mounting block of the extraction tube moving module is provided with a feeding clamping jaw group. The feeding clamping jaw group comprises n mechanical clamping jaws which are sequentially arranged. The mechanical clamping jaw is used for grabbing a single nucleic acid magnetic bead extraction tube. The extraction tube moving module is used for driving the feeding clamping jaw set to move to the position right above the first storage platform or the second storage platform and the position right above the feeding station.

Preferably, the flattening assembly comprises a flattening cylinder and a pressing plate. The flattening cylinder is fixed on the frame. A pressing plate is fixed on the downward push-out rod of the flattening cylinder. The pressing plate is positioned right above the flattening and material cleaning station.

Clear material subassembly clear material cylinder, clear material picture peg, clear material removal module, clear material collude board and waste material cell body including the jacking. The waste material groove body is fixed on the frame and close to the flattening and material cleaning station. The jacking material cleaning air cylinder is fixed on the frame and is positioned right below the flattening material cleaning station. N material cleaning inserting plates which are sequentially arranged at intervals are fixed on an upward push rod of the jacking material cleaning cylinder; n is the number of placing positions on the extracting tube jig. The relative positions of the n material cleaning inserting plates correspond to the relative positions of the n placing positions of the extracting pipe jig.

The material cleaning moving module is arranged on the rack; the material cleaning hook plate is arranged on the moving part of the material cleaning moving module; the bottom surface of the material cleaning hook plate is provided with a hook groove. The material clearing hook plate can send the nucleic acid magnetic bead extraction tube pushed out of the extraction tube jig into the waste material tank under the driving of the material clearing moving module

Preferably, magnetic bead filling mechanism include magnetic bead groove sideslip module, magnetic bead groove, magnetic bead bucket driving motor, magnetic bead bucket mount pad, magnetic bead bucket, pipetting gun and pipetting gun remove the subassembly. The liquid-transfering guns of the n channels are arranged on the moving part of the liquid-transfering gun moving assembly. n is the number of placing positions on the extracting tube jig. The relative positions of the n output ports of the pipette correspond to the relative positions of the n placing positions of the extracting tube jig. The pipetting gun moving assembly can move the pipetting gun to a magnetic bead groove and a magnetic bead filling station right above.

The magnetic bead bucket mounting seat is rotatably connected to the rack and is driven to turn over by a magnetic bead bucket driving motor. The magnetic bead bucket is installed on the magnetic bead bucket mount pad. An electromagnetic valve is arranged at the output port of the magnetic bead barrel. The magnetic bead groove is arranged on the rack through the magnetic bead groove transverse moving module; the magnetic bead groove transverse moving module can drive the magnetic bead groove to switch between a magnetic bead pouring position and a magnetic bead sucking position. The magnetic bead pouring position of the magnetic bead groove corresponds to the outlet position of the magnetic bead barrel; the magnetic bead sucking position of the magnetic bead groove corresponds to the position of the pipette.

Annotate liquid mechanism include the jacking mount pad, annotate liquid jacking cylinder, annotate liquid jacking piece, connect the cistern, annotate liquid sideslip mount pad, annotate liquid sideslip cylinder, annotate the liquid board and annotate the liquid pipe. The input port of the liquid injection plate is connected to a liquid source through a liquid injection pipe. The liquid receiving tank is arranged on the frame. The horizontal liquid injection transverse cylinder is arranged on the frame. The liquid injection plate is installed on a push-out rod of the liquid injection transverse moving cylinder and fixed. The liquid injection transverse cylinder can drive the liquid injection plate to be switched between the position right above the liquid injection station and the position right above the liquid receiving groove. The liquid injection jacking cylinder is installed on the frame through a jacking installation seat. A liquid injection jacking block is fixed on the upward pushing rod of the liquid injection jacking cylinder; the liquid injection jacking block is used for pushing up each nucleic acid magnetic bead extraction tube in the extraction tube jig, so that the nucleic acid magnetic bead extraction tubes are close to the liquid outlet of the liquid injection plate.

Preferably, the film sealing hot-pressing mechanism comprises a film supply assembly, a film transfer assembly and a hot-pressing assembly. The film supply assembly comprises a film side stop lever, a film supply mounting seat, a film conveying lifting module and a film stacking support. A plurality of vertically arranged diaphragm side stop rods are fixed on the frame. Each diaphragm side stop lever encloses a diaphragm limiting area which is rectangular. The film feeding lifting module is arranged on the frame. And a membrane stacking bracket is arranged on the moving part of the membrane conveying lifting module. The membrane stacking support is positioned in the membrane limiting area and used for supporting the stacked aluminum membranes.

The membrane transferring assembly comprises a membrane moving module, a sucker mounting plate and a sucker. The sucking disc mounting panel is installed on diaphragm removes module removal portion. A plurality of sucking discs that set up all install on the sucking disc mounting panel down. The diaphragm moving module can drive the sucker mounting plate to move to the position right above the diaphragm stacking support and the position right above the hot pressing station.

The hot pressing assembly comprises a hot pressing plate, a hot pressing lifting module, a hot pressing jacking frame and a hot pressing jacking module. The hot-pressing lifting module is arranged on the frame. The hot-pressing jacking module is arranged on the frame. The hot pressing plate is arranged on the moving part of the hot pressing lifting module. The hot pressing plate is provided with a heating element. The hot pressing jacking frame is arranged on the moving part of the hot pressing jacking module. The hot pressing plate is positioned right above the hot pressing station. The hot-pressing jacking frame is positioned right below the hot-pressing station. In the working process, the hot-pressing jacking frame is lifted to support the extraction pipe jig on the hot-pressing station; the hot press plate starts to heat and moves downwards, and the aluminum film is extruded, so that the aluminum film is bonded with the opening of each nucleic acid magnetic bead extraction tube.

Preferably, the cold press mechanism comprises a cold pressing plate, a cold pressing heat dissipation plate, a cold pressing fan, a cold pressing mounting bracket, a cold pressing lifting module, a cold pressing jacking frame and a cold pressing jacking module. The cold pressing lifting module and the cold pressing jacking module are both installed on the rack. Install on the removal portion of cold pressing lifting die set. The bottom surface of the cold-pressing heat dissipation plate is fixed with a cold-pressing plate, and the top surface is provided with a cold-pressing fan. The cold pressing jacking frame is installed on the moving part of the cold pressing jacking module. The cold pressing plate is positioned right above the cold pressing station. The cold pressing jacking frame is positioned right below the cold pressing station. In the working process, the cold pressing jacking frame is lifted to support the extraction pipe jig on the cold pressing station; the cold pressing plate moves downwards to press the aluminum film adhered to each nucleic acid magnetic bead extraction tube, so that the temperature of the aluminum film is reduced.

Preferably, the film cutting mechanism comprises a cutter, a cutter mounting seat and a film cutting moving module. The cutter mount pad is installed in the removal portion of cutting the membrane removal module. N-1 cutters are fixed on the cutter mounting seat. n is the number of placing positions on the extracting tube jig. The relative position of each cutter corresponds to the clearance position between each placing position on the extracting tube jig respectively. The film cutting moving module can drive the cutter to lift and transversely move. The blade of cutter is protruding V-arrangement down, and the bottom is provided with the sharp-pointed point. In the working process, the V-shaped cutting edge of the cutter firstly cuts into the aluminum film from the middle part of the aluminum film and then moves towards the two side edges of the aluminum film in sequence to cut off the aluminum film.

Preferably, the blanking mechanism comprises a blanking jacking assembly, a blanking transfer assembly and a blanking conveying assembly. The blanking jacking assembly comprises a blanking plugboard and a blanking jacking module. N unloading picture pegs that the interval set up in proper order all install on the removal portion of unloading jacking module. n is the number of placing positions on the extracting tube jig. The relative positions of the n blanking inserting plates correspond to the relative positions of the n placing positions of the extracting pipe jig. The n blanking plugboards can extend upwards into the n placing positions of the extraction tube jig at the blanking station, and the n nucleic acid magnetic bead extraction tubes in the extraction tube jig are ejected out.

The blanking transfer assembly comprises a blanking clamping jaw group and a blanking moving module. The unloading clamping jaw group is installed on the terminal installation piece of unloading removal module. The unloading removes the module and can drive unloading clamping jaw group and go up and down, sideslip motion and rotatory around vertical axis, can drive unloading clamping jaw group and remove directly over to the unloading station, directly over unloading conveying assembly. The unloading clamping jaw group can simultaneously clamp n nucleic acid magnetic bead extraction tubes on the extraction tube jig.

The blanking conveying assembly comprises a conveyor and a conveying striker plate. The conveyer is installed on the frame. The input end of the conveyor is provided with a U-shaped conveying striker plate.

The working process of the full-automatic nucleic acid magnetic bead extraction tube filling and membrane sealing equipment is as follows:

step one, a feeding mechanism puts a nucleic acid magnetic bead extraction tube into an extraction tube fixture of a feeding station.

And step two, rotating the turntable, and transferring the extraction tube jig of the feeding station to a flattening and material cleaning station. A flattening component of the flattening and material cleaning mechanism pushes and presses each nucleic acid magnetic bead extraction tube in the extraction tube jig downwards; if the pressing and the pressing cannot be carried out in place, the cleaning component removes the nucleic acid magnetic bead extraction tubes from the pressing and extraction tube jig.

And step three, rotating the turntable, and transferring the extraction tube jig of the flattening and material cleaning station to a magnetic bead filling station. And the magnetic bead filling mechanism fills magnetic beads into the nucleic acid magnetic bead extraction tube.

And step four, rotating the turntable, and transferring the extraction tube jig of the magnetic bead filling station to the liquid injection station. The liquid injection mechanism injects a reagent into the nucleic acid magnetic bead extraction tube.

And fifthly, rotating the turntable, and transferring the extraction tube jig of the liquid injection station to a hot pressing station. And an aluminum film is placed on each nucleic acid magnetic bead extraction tube by the film sealing hot-pressing mechanism, and is adhered to the opening of the nucleic acid magnetic bead extraction tube in a hot-pressing mode.

And step six, rotating the turntable, and transferring the extraction tube jig of the hot pressing station to the cold pressing station. The cold pressing mechanism extrudes the aluminum film, and reduces the temperature of the aluminum film through heat transfer.

And step seven, rotating the turntable, and transferring the extraction tube jig of the cold pressing station to the film cutting station. The membrane cutting mechanism cuts the aluminum membrane of the nucleic acid magnetic bead extraction tube between every two nucleic acid magnetic bead extraction tubes.

And step eight, rotating the turntable, and transferring the extraction tube jig at the film cutting station to a blanking station. The feeding mechanism takes out the nucleic acid magnetic bead extraction tubes in the extraction tube jig.

The invention has the beneficial effects that:

1. the full-automatic filling, film sealing, cutting and blanking process of the nucleic acid magnetic bead extraction tube can be completed fully automatically, automatic production is realized, manpower is reduced, and production efficiency is improved.

2. The feeding mechanism can arrange scattered nucleic acid magnetic bead extraction tubes one by one, and the plurality of nucleic acid magnetic bead extraction tubes are fed to the extraction tube jig of the feeding station at one time, so that the feeding speed is increased, and the influence of the feeding process on the overall operation efficiency of equipment is avoided. In addition, the structure that the sideslip exchanges subassembly in turn in the feed mechanism only used hold-in range cooperation cam groove has just realized two and has deposited the platform and realize the position exchange under the condition that does not carry out lateral shifting for the row of extraction tube is put things in good order and is got the material loading and can go on simultaneously, each other not influence.

3. The invention is provided with a flattening and material cleaning mechanism, and judges whether the extraction tube is placed in place or not by utilizing the mode that whether a pressing plate can be pressed in place or not; under the condition that the extraction tube is not placed in place, utilize the hollow out construction of picture peg cooperation tool bottom, release the tool with the extraction tube to utilize and collude the board and shift out the extraction tube to the waste material cell body in, improved the stability of equipment operation.

4. The invention applies hot pressing and cold pressing operations to the aluminum film and the extraction tube in sequence, so that the aluminum film can be tightly adhered to the opening of the extraction tube. Meanwhile, one hot and cold pressing operation can simultaneously seal a plurality of extraction tubes. At the in-process of hot pressing and the operation of colding pressing, utilize the elevating platform in the jacking structure cooperation extraction tube tool, realize the support to each extraction tube, avoid pressure direct action and carousel to avoid the carousel to take place the problem of warping in long-term operation, improved the stability and the life-span of equipment.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention.

Fig. 2 is a schematic top view of the present invention.

FIG. 3 is a schematic diagram of the station switching mechanism of the present invention.

Fig. 4 is a perspective view of the extraction tube jig of the present invention.

Fig. 5 is a cross-sectional view of the extractor tube jig of the present invention.

Fig. 6 is a perspective view of the feeding mechanism of the present invention.

FIG. 7 is a schematic view of a traverse alternate assembly of the present invention.

Fig. 8 is a schematic view of the flattening and cleaning mechanism of the present invention.

FIG. 9 is a schematic view of a magnetic bead filling mechanism according to the present invention.

FIG. 10 is a schematic view of the priming mechanism of the present invention.

FIG. 11 is a first schematic view of a film sealing and hot-pressing mechanism according to the present invention.

FIG. 12 is a second schematic view of a film sealing thermal pressing mechanism according to the present invention.

Figure 13 is a schematic diagram of the cold press mechanism of the present invention.

FIG. 14 is a schematic view of a film cutting mechanism according to the present invention.

Fig. 15 is a first schematic view of the blanking mechanism of the present invention.

Fig. 16 is a second schematic view of the blanking mechanism of the present invention.

Detailed Description

The invention is further described below with reference to the accompanying drawings.

As shown in fig. 1 and 2, a full-automatic nucleic acid magnetic bead extraction tube filling and film sealing device comprises a feeding mechanism 1, a flattening and material cleaning mechanism 2, a magnetic bead filling mechanism 3, a liquid injection mechanism 4, a film sealing hot-pressing mechanism 5, a cold-pressing mechanism 6, a film cutting mechanism 7, a blanking mechanism 8, a station switching mechanism 9 and a frame 10.

As shown in fig. 3, the station switching mechanism 9 is fixed to the top of the frame 10. The station switching mechanism 9 has eight stations, which are a feeding station, a flattening and material cleaning station, a magnetic bead filling station, a liquid injection station, a hot pressing station, a cold pressing station, a film cutting station and a discharging station. The positions of the feeding mechanism 1, the flattening and cleaning mechanism 2, the magnetic bead filling mechanism 3, the liquid injection mechanism 4, the film sealing hot-pressing mechanism 5, the cold-pressing mechanism 6, the film cutting mechanism 7 and the blanking mechanism 8 correspond to the eight stations respectively.

The station switching mechanism 9 comprises a turntable 9-2, an extraction tube jig 9-1 and a driving mechanism. The horizontally arranged rotary table 9-2 is rotatably connected to the frame 10 and is driven to rotate by a driving mechanism. The driving mechanism adopts a motor with a speed reducer. Eight extraction pipe jigs 9-1 which are uniformly distributed along the circumferential direction of the axis of the rotary table 9-2 are arranged on the top surface of the rotary table 9-2; the extraction pipe jig 9-1 is arranged on a through groove formed in the rotary table 9-2. The extraction tube jig 9-1 is used for placing the filled nucleic acid magnetic bead extraction tube. The rotation of the rotary table 9-2 can drive each extraction pipe jig 9-1 to sequentially pass through eight stations. Six placing positions are arranged on the extraction tube jig 9-1; each placing position can be used for placing a nucleic acid magnetic bead extraction tube 11. The nucleic acid magnetic bead extraction tube 11 in this embodiment is specifically a six-connection tube. The bottoms of the placing positions of the extracting tube jigs 9-1 are all arranged in a hollow manner, and the inserting plates can be used for extending into the placing positions from the lower direction to eject the nucleic acid magnetic bead extracting tubes 11 out of the placing positions.

As shown in fig. 4 and 5, the extraction tube jig 9-1 includes a jig base and a jig elevating table. The jig lifting table is connected to the jig base in a sliding mode. The jig base comprises an extraction tube base 9-1-1, a film cutting guide plate 9-1-2 and a jig guide shaft 9-1-4. The extraction tube base 9-1-1 is fixed on the turntable. A plurality of vertically arranged jig guide shafts 9-1-4 are fixed at the top of the extraction tube base 9-1-1; the film cutting guide plate 9-1-2 is fixed with the top end of each jig guide shaft 9-1-4.

Six placing through grooves which are arranged at equal intervals in sequence are arranged on the film cutting guide plate 9-1-2. Cutter groove of stepping down has all been seted up between two arbitrary logical grooves of placing. The jig lifting platform comprises an extraction tube placing box 9-1-3 and a placing box linear bearing 9-1-5. The extraction tube placing box 9-1-3 is connected to the jig guide shaft 9-1-4 in a sliding manner through a placing box linear bearing 9-1-5; six placing positions are arranged on the extracting tube placing box 9-1-3. Each placing position is aligned with the six placing through grooves respectively. The bottoms of the placing positions are provided with the yielding grooves, so that the inserting plates of the flattening and cleaning mechanism 2 and the blanking mechanism 8 can extend into the placing positions from bottom to top, and the nucleic acid magnetic bead extraction tubes 11 are pushed out of the extraction tube jig 9-1.

As shown in fig. 6, the loading mechanism 1 includes a pre-integration assembly and an extraction pipe transfer assembly. The front integration component has two. The two preposed integration components are used for arranging the stacked nucleic acid magnetic bead extraction tubes 11 into an extraction tube array of six groups; the extraction pipe transfer assembly is only provided with one extraction pipe transfer assembly and is used for conveying the extraction pipe queues arranged by the two preposed integration assemblies to an extraction pipe jig 9-1 of the feeding station.

The front-mounted integration component comprises a vibration disc 1-1, a grabbing component 1-2 and a transverse movement alternating component 1-3. The vibrating disk 1-1 is arranged on the frame and used for arranging and outputting the stacked nucleic acid magnetic bead extraction tubes one by one. The grabbing component adopts a scara manipulator and is used for grabbing the nucleic acid magnetic bead extraction tubes one by one on the storage table of the transverse moving alternate component 1-3 to form a row of six extraction tube queues so as to place six nucleic acid magnetic bead extraction tubes on the extraction tube jig 9-1 at the feeding station at one time, and the problems that the feeding step consumes too long time and the overall operation efficiency of the equipment is influenced are avoided.

As shown in FIG. 7, the traverse motion alternation and interchange assembly 1-3 comprises a traverse motion base 1-3-1, a cam groove 1-3-2, a synchronous belt 1-3-3, a first roller 1-3-4, a lifting frame 1-3-5, an alternation guide shaft 1-3-6, a first storage table 1-3-7, a sliding plate 1-3-8, a linear guide rail 1-3-9, a second storage table 1-3-10 and a synchronous wheel 1-3-11. The transverse moving base 1-3-1 is fixed on the frame. A lifting guide plate is fixed on the transverse moving base 1-3-1; the lifting guide plate is provided with a cam groove 1-3-2. Four synchronizing wheels 1-3-11 which are arranged in a rectangular shape in a vertical plane are rotatably connected to the lifting guide plate. The four synchronous wheels 1-3-11 are in transmission connection through synchronous belts 1-3-3. One of the synchronizing wheels 1-3-11 is driven by a motor. The upper and lower groups of linear guide rails 1-3-9 are fixed on the transverse moving base 1-3-1. Each group of linear guide rails 1-3-9 comprises two linear guide rails 1-3-9 which are equal in height and are arranged at intervals.

The second storage table 1-3-10 is slidably connected to the linear guide 1-3-9 located above by means of a slide. The sliding plates 1-3-8 are connected with the linear guide rails 1-3-9 positioned below in a sliding mode through sliding blocks. The lifting frame 1-3-5 and the sliding plate 1-3-8 form a sliding pair sliding along the vertical direction through a linear bearing and an alternate guide shaft 1-3-6. The bottom of the lifting frame 1-3-5 is rotatably connected with a first roller 1-3-4. The first roller 1-3-4 is disposed in the cam groove 1-3-2. The top of the lifting frame 1-3-5 is fixed with a first storage table 1-3-7. Six storage positions are arranged on the first storage table 1-3-7 and the second storage table 1-3-10. The relative position of each storage position corresponds to the relative position of each placing position on the extracting tube jig respectively.

The cam groove 1-3-2 has a shape with two ends higher and a middle lower. When the first roller 1-3-4 is positioned at the highest position of the end part of the cam groove 1-3-2, the first storage table 1-3-7 and the second storage table 1-3-10 are equal in height. When the first roller 1-3-4 is at the lowest position in the middle of the cam groove 1-3-2, the top of the first storage table 1-3-7 is lower than the bottom of the second storage table 1-3-10, and the first storage table 1-3-7 can pass right under the second storage table 1-3-10.

The sliding plate 1-3-8, the second storage table 1-3-10 and the two horizontal sections of the synchronous belt are respectively fixed. When the synchronous belt moves, the sliding plate 1-3-8 and the second storage table 1-3-10 move reversely at a constant speed to drive the first storage table 1-3-7 and the second storage table 1-3-10 to exchange positions. The two positions of the first storage table 1-3-7 and the second storage table 1-3-10 are respectively used for feeding the transverse moving alternate assembly 1-3 and taking the extracting pipe transfer assembly.

The extraction pipe transfer assembly comprises an extraction pipe moving module and a feeding clamping jaw set 1-5. And a feeding clamping jaw group 1-5 is arranged on the tail end mounting block of the extraction pipe moving module. The feeding clamping jaw group 1-5 comprises six mechanical clamping jaws which are arranged in sequence. The mechanical clamping jaws all adopt clamping jaw air cylinders and can grab the nucleic acid magnetic bead extraction tube. The relative positions of the six mechanical clamping jaws correspond to the relative positions of the mounting positions of the six extraction tubes on the first storage table 1-3-7 and the second storage table 1-3-10, so that the six nucleic acid magnetic bead extraction tubes can be clamped at one time.

The extraction tube moving module comprises a transverse sliding table 1-4 and a lifting sliding table 1-6. The transverse sliding tables 1-4 and the lifting sliding tables 1-6 are linear electric sliding tables. The lifting sliding table 1-6 is arranged on the sliding block of the transverse sliding table 1-4. The extraction tube moving module can drive the feeding clamping jaw group 1-5 to move transversely and move in a lifting mode, six nucleic acid magnetic bead extraction tubes are lifted, and the six nucleic acid magnetic bead extraction tubes are transferred to an extraction tube jig 9-1 of a feeding station.

As shown in fig. 8, the flattening and material-clearing mechanism 2 comprises a flattening and material-clearing upright post 2-1, a flattening component, a material-clearing component and a flattening cylinder mounting plate 2-5. The flattening cylinder mounting plate 2-5 is fixed on the frame through the flattening material cleaning upright post 2-1. The flattening assembly comprises a flattening cylinder 2-7 and a pressing plate 2-8. The flattening cylinder 2-7 is fixed on the flattening cylinder mounting plate 2-5. A pressing plate 2-8 is fixed on a downward pushing rod of the flattening cylinder 2-7. The pressing plate 2-8 is positioned right above the flattening and material cleaning station; the pressing plate 2-8 moves downwards to extrude and flatten all the nucleic acid magnetic bead extraction tubes on the extraction tube jig 9-1 of the material cleaning station.

The material cleaning component comprises a jacking material cleaning cylinder 2-2, a material cleaning inserting plate 2-3, a material cleaning horizontal cylinder 2-4, a material cleaning lifting cylinder 2-6, a material cleaning hook plate 2-9 and a waste material tank body 2-10. The waste material tank body 2-10 is fixed on the frame and close to the flattening and material cleaning station. The jacking material cleaning cylinder 2-2 is fixed on the frame and is positioned right below the flattening material cleaning station. Six material cleaning inserting plates 2-3 which are sequentially arranged at intervals are fixed on an upward push rod of the jacking material cleaning cylinder 2-2; the relative positions of the six material cleaning inserting plates 2-3 correspond to the relative positions of six placing positions of the extraction pipe jig 9-1. The six material cleaning inserting plates 2-3 can extend upwards into six placing positions of the extraction tube jig 9-1 at the flattening material cleaning station, and push out six nucleic acid magnetic bead extraction tubes in the extraction tube jig 9-1.

The material cleaning lifting cylinder 2-6 is fixed on the flattening cylinder mounting plate 2-5; the vertical push-out rod of the material cleaning lifting cylinder 2-6 is fixed with the cylinder body of the material cleaning horizontal cylinder 2-4. The horizontal push-out rod of the material cleaning horizontal cylinder 2-4 is fixed with one side edge of the material cleaning hook plate 2-9. The bottom surfaces of the material cleaning hook plates 2-9 are provided with hook grooves.

When the nucleic acid magnetic bead extraction tube in the extraction tube jig 9-1 of the flattening and material cleaning station needs to be cleaned, the material cleaning lifting cylinder 2-6 and the material cleaning horizontal cylinder 2-4 drive the material cleaning insertion plate to move to the position right above the flattening and material cleaning station. The top ends of the nucleic acid magnetic bead extraction pipes are pushed to extend into the material hooking grooves of the material cleaning hook plates 2-9 by the material cleaning inserting plate, when the nucleic acid magnetic bead extraction pipes are pushed to be separated from the extraction pipe jigs 9-1, the material cleaning horizontal cylinder 2-4 moves transversely, the material cleaning hook plates 2-9 drive the nucleic acid magnetic bead extraction pipes to move transversely, and the nucleic acid magnetic bead extraction pipes are conveyed into the waste material groove bodies 2-10. Thereby realizing automatic material cleaning when the nucleic acid magnetic bead extraction tube is not correctly placed.

As shown in fig. 9, the magnetic bead filling mechanism 3 includes a magnetic bead groove traversing module 3-1, a magnetic bead groove 3-2, a magnetic bead bucket driving motor 3-3, a magnetic bead bucket mounting seat 3-4, a magnetic bead bucket 3-5, a pipette 3-6, and a pipette moving assembly 3-7. The liquid-transfering gun moving assembly 3-7 adopts a two-axis moving platform, the moving direction of which is Y-axis and Z-axis, and the moving is realized by two electric sliding tables which are vertically arranged together. The liquid-transfering gun 3-6 of six-channel is mounted on two-freedom-degree moving plate of liquid-transfering gun moving component 3-7. The relative positions of the six output ports of the liquid-transfering gun 3-6 correspond to the relative positions of the six placing positions of the extracting tube jig 9-1. The liquid-transfering gun moving assembly 3-7 can move the liquid-transfering gun 3-6 to a magnetic bead groove for magnetic bead supplement, and move the liquid-transfering gun to a position right above a magnetic bead filling station for filling magnetic beads for six nucleic acid magnetic bead extraction tubes in the extraction tube jig 9-1.

The magnetic bead barrel mounting seat 3-4 is rotatably connected to the rack 10 and is driven to turn over by a magnetic bead barrel driving motor 3-3. The magnetic bead barrel 3-5 is arranged on the magnetic bead barrel mounting seat 3-4. An electromagnetic valve is arranged at the output port of the magnetic bead barrel 3-5. The magnetic bead barrel driving motor 3-3 can drive the magnetic bead barrel 3-5 to turn over in a reciprocating mode so as to shake up magnetic beads in the magnetic bead barrel 3-5.

The magnetic bead groove 3-2 is arranged on the rack 10 through the magnetic bead groove transverse moving module 3-1; the magnetic bead groove transverse moving module 3-1 adopts an electric sliding table and can drive the magnetic bead groove 3-2 to switch between a magnetic bead pouring position and a magnetic bead sucking position. The magnetic bead pouring position of the magnetic bead groove 3-2 corresponds to the outlet position of the magnetic bead barrel 3-5 and is used for receiving the magnetic beads poured out from the magnetic bead barrel 3-5; the magnetic bead sucking position of the magnetic bead groove 3-2 corresponds to the position of each pipette 3-6 and is used for the pipette 3-6 to suck magnetic beads.

As shown in figure 10, the liquid injection mechanism 4 comprises a jacking installation seat 4-1, a liquid injection jacking cylinder 4-2, a liquid injection jacking block 4-3, a liquid receiving groove 4-4, a liquid injection transverse moving installation seat 4-5, a liquid injection transverse moving cylinder 4-6, a liquid injection plate 4-7 and a liquid injection pipe 4-8. The input port of the liquid injection plate 4-7 is connected to a liquid source (for outputting reagents for nucleic acid detection including lysis solution, deproteinization solution, and washing solution) through a liquid injection tube 4-8. The liquid receiving tank 4-4 is arranged on the frame. The horizontally arranged liquid injection transverse moving cylinder 4-6 is arranged on the rack 10 through the liquid injection transverse moving mounting seat 4-5. The liquid injection plate 4-7 is fixed on a push-out rod of the liquid injection transverse moving cylinder 4-6. The liquid injection transverse moving cylinder 4-6 can drive the liquid injection plate 4-7 to switch between the position right above the liquid injection station and the position right above the liquid receiving groove 4-4. The liquid injection jacking cylinder 4-2 is installed on the machine frame 10 through a jacking installation seat 4-1. A liquid injection jacking block 4-3 is fixed on an upward pushing rod of the liquid injection jacking cylinder 4-2; when the liquid injection jacking cylinder 4-2 is pushed out, the lifting table of the internal jig lifting platform of the extraction tube jig 9-1 of the liquid injection station can be pushed up through the jacking block 4-3, so that each nucleic acid magnetic bead extraction tube 11 is closer to the liquid outlet of the liquid injection plate 4-7, and the liquid injection stability is improved.

As shown in fig. 11 and 12, the film sealing hot-pressing mechanism 5 includes a hot-pressing mounting bracket 5-3, a film supply assembly, a film transfer assembly and a hot-pressing assembly. The hot-press mounting bracket 5-3 is fixed on the frame. The film supply assembly is used for placing an aluminum film on the extraction pipe jig 9-1 of the hot pressing station; the aluminum film can cover the openings of all the nucleic acid magnetic bead extraction tubes 11 on the extraction tube jig 9-1. The hot-pressing assembly is used for applying hot pressing between the aluminum film and the opening of the nucleic acid magnetic bead extraction tube 11, so that the aluminum film is adhered to the opening of the nucleic acid magnetic bead extraction tube 11.

The film supply assembly comprises a film side stop lever 5-1, a film bottom plate 5-2, a film supply mounting seat 5-4, a film feeding lifting module 5-5 and a film stacking support 5-6. The diaphragm bottom plate 5-2 is fixed on the frame 10; a plurality of membrane side stop rods 5-1 which are vertically arranged are all fixed on the membrane bottom plate 5-2. Each diaphragm side stop lever 5-1 surrounds a rectangular diaphragm limiting area. The film feeding lifting module 5-5 is arranged on the frame 10 through a film supply mounting seat 5-4. The film feeding lifting module 5-5 adopts an electric sliding table. A membrane stacking support 5-6 is fixed on the sliding part of the membrane feeding lifting module 5-5. The membrane stacking supports 5-6 are located in the membrane limiting area and used for supporting the stacked aluminum membranes.

The membrane transferring assembly comprises a membrane moving module, a sucker mounting plate 5-13 and a sucker 5-23. The diaphragm moving module can drive the sucker mounting plates 5-13 to move transversely and lift. The plurality of suckers 5-23 arranged downwards are all arranged on the sucker mounting plates 5-13. The diaphragm moving module can drive the sucker mounting plate 5-13 to move to the position right above the diaphragm bottom plate 5-2 and the position right above the hot pressing station. The membrane moving module comprises a membrane feeding lifting cylinder 5-10, a membrane feeding transverse moving cylinder 5-11 and a membrane feeding transverse moving mounting plate 5-12. And a film feeding lifting cylinder 5-10 is fixed at the top of the hot-pressing mounting bracket 5-3. The pushing rod of the film feeding lifting cylinder 5-10 is arranged downwards and is fixed with the film feeding transverse mounting plate 5-12. A film feeding traversing cylinder 5-11 is fixed at the bottom of the film feeding traversing mounting plate 5-12. The sucking disc mounting plate 5-13 is fixed on the sliding part of the film feeding and traversing cylinder 5-11.

The hot pressing assembly comprises a hot pressing plate 5-9, a hot pressing lifting module, a hot pressing jacking frame 5-14 and a hot pressing jacking module. The hot-pressing lifting module is arranged on the hot-pressing mounting bracket 5-3. The hot-pressing jacking module is arranged on the frame. The hot pressing plates 5-9 are arranged on the moving part of the hot pressing lifting module. Heating elements are mounted in the hot platens 5-9. The hot-pressing jacking frames 5-14 are arranged on the moving part of the hot-pressing jacking module. The hot pressing plates 5-9 are positioned right above the hot pressing station. The hot-pressing jacking frames 5-14 are positioned right below the hot-pressing station. In the working process, the hot-pressing jacking frame 5-14 is lifted to support the extraction pipe jig 9-1 on the hot-pressing station; the hot press plates 5 to 9 start to heat and move downward, and press the aluminum film so that the aluminum film is adhered to the openings of the respective nucleic acid magnetic bead extraction tubes 11.

The hot-pressing lifting module comprises a hot-pressing cylinder mounting base 5-7 and a hot-pressing cylinder 5-8. The hot pressing cylinder 5-8 is fixed on the hot pressing mounting bracket 5-3 through a hot pressing cylinder mounting seat 5-7; the push-out rod of the hot-pressing cylinder 5-8 is arranged downwards and fixed with the hot-pressing plate 5-9.

The hot-pressing jacking module comprises 5-15 parts of a hot-pressing jacking base, 5-16 parts of a hot-pressing jacking sliding rail, 5-17 parts of a hot-pressing jacking block, 5-18 parts of a second roller, 5-19 parts of a wedge inserting plate, 5-20 parts of an inserting plate guide seat, 5-21 parts of an inserting plate pulling seat and 5-22 parts of a hot-pressing jacking cylinder. The inserting plate guide seats 5-20 are fixed on the frame through hot-pressing jacking bases 5-15. The inclined wedge inserting plates 5-19 are connected on the inserting plate guide seats 5-20 in a sliding mode. The hot-pressing jacking cylinders 5-22 are fixed on the frame 10. The push-out rod of the hot-pressing jacking cylinder 5-22 is horizontally arranged and fixed with the inclined wedge inserting plate 5-19 through an inserting plate pulling seat 5-21. The outer ends of the top surfaces of the inclined wedge inserting plates 5 to 19 are provided with inclined guide surfaces. The hot-pressing jacking frame 5-14 is connected to the hot-pressing jacking base 5-15 in a sliding manner along the vertical direction through a hot-pressing jacking sliding rail 5-16. The hot-pressing jacking blocks 5-17 are fixed on the hot-pressing jacking frames 5-14. The bottom of the hot-pressing jacking block 5-17 is rotatably connected with a second roller 5-18. The second roller 5-18 contacts with the top surface of the inclined wedge inserting plate 5-19. In the hot pressing process, the second rollers 5-18 roll on the inclined guide surfaces of the inclined wedge inserting plates 5-19 through the telescopic motion of the hot pressing jacking cylinders 5-22 to drive the hot pressing jacking frames 5-14 to ascend to support a jig lifting platform of the extraction pipe jig 9-1 on a hot pressing station, so that the downward pressure applied by a hot pressing plate in the hot pressing process is prevented from acting on the rotary table 9-2, the rotary table 9-2 is prevented from deforming, and the running stability of equipment is improved.

As shown in fig. 13, the cold pressing mechanism 6 comprises a cold pressing plate 6-1, a cold pressing heat dissipation plate 6-2, a cold pressing fan 6-3, a cold pressing mounting bracket, a cold pressing lifting module, a cold pressing jacking frame 6-8 and a cold pressing jacking module. The cold-pressed mounting bracket comprises a cold-pressed mounting plate 6-6 and a cold-pressed support 6-7. The cold-pressed mounting plate 6-6 is fixed with the frame 10 through a plurality of cold-pressed pillars 6-7.

The cold-pressing lifting module is arranged on the cold-pressing mounting plate 6-6. The cold pressing jacking module is arranged on the frame. Install on the removal portion of cold pressing lifting die set. The cold pressing plate 6-1 is fixed on the bottom surface of the cold pressing heat dissipation plate 6-2, and the cold pressing fan 6-3 is installed on the top surface. And the cold pressing jacking frame 6-8 is arranged on the moving part of the cold pressing jacking module. The cold pressing plate 6-1 is positioned right above the cold pressing station. And the cold pressing jacking frame 6-8 is positioned right below the cold pressing station. In the working process, the cold pressing jacking frame 6-8 is lifted to support the extraction pipe jig 9-1 on the cold pressing station; the cold pressing plate 6-1 moves downwards to press the aluminum film adhered to the openings of the nucleic acid magnetic bead extraction tubes 11, so that the temperature of the aluminum film is reduced, and the aluminum film is more tightly adhered to the nucleic acid magnetic bead extraction tubes 11.

The cold pressing lifting module comprises a cold pressing cylinder mounting seat 6-5 and a cold pressing cylinder 6-4. The cold pressing cylinder 6-4 is fixed on the cold pressing mounting bracket through a cold pressing cylinder mounting seat 6-5; the push-out rod of the cold pressing cylinder 6-4 is arranged downwards and fixed with the cold pressing heat dissipation plate 6-2. The structure of the cold pressing jacking module is the same as that of the hot pressing jacking mechanism.

As shown in fig. 14, the film cutting mechanism 7 includes a cutter 7-1, a cutter mounting base 7-2 and a film cutting moving module. The cutter mounting seat 7-2 is arranged on the moving part of the film cutting moving module. Five cutters 7-1 are fixed on the cutter mounting seat 7-2. The relative position of each cutter 7-1 corresponds to the gap position between each placing position on the extraction tube jig 9-1. The film cutting moving module can drive the cutter to lift and transversely move. The cutting edge of the cutter 7-1 is in a downward convex V shape, and the bottom is provided with a sharp point. The V-shaped cutting edge of the cutter 7-1 enables the cutter 7-1 to cut in from the middle of the aluminum film firstly and then move towards the two side edges of the aluminum film in sequence to cut off the aluminum film, and the problem that the aluminum film is damaged due to cutting in from the edge of the aluminum film in the prior art is solved.

The film cutting moving module comprises 7-3 parts of a film cutting transverse moving guide rail, 7-4 parts of a cylinder connecting piece, 7-5 parts of a film cutting transverse moving cylinder, 7-6 parts of a film cutting lifting cylinder, 7-7 parts of a film cutting top plate, 7-8 parts of a film cutting lifting plate, 7-9 parts of a film cutting guide rod and 7-10 parts of a film cutting base. The film cutting base 7-10 is fixed on the frame 10. The bottom ends and the top ends of the two film cutting guide rods 7-9 are respectively fixed with the film cutting base 7-10 and the film cutting top plate 7-7. The film cutting lifting plate 7-8 is connected on the film cutting guide rod 7-9 in a sliding manner. The film cutting and traversing cylinder 7-5 is fixed on the film cutting lifting plate 7-8. The cutter mounting seat 7-2 is connected on the film cutting lifting plate 7-8 in a sliding way through a film cutting transverse moving guide rail 7-3. The cutter mounting seat 7-2 is fixed with a push-out rod of the film cutting traversing cylinder 7-5. The film cutting and traversing cylinder 7-5 adopts a double-stroke cylinder and has three working positions. When the film cutting transverse cylinder 7-5 is in the middle working position, the cutting edge of the cutter is positioned between the middle parts of the two corresponding placing positions of the extraction tube jig 9-1 on the film cutting station, and the position is used for puncturing the middle part of the aluminum film.

As shown in fig. 15 and 16, the blanking mechanism 8 includes a blanking jacking assembly, a blanking transferring assembly and a blanking conveying assembly. The blanking jacking assembly comprises blanking plugboards 8-11 and a blanking jacking module. Six blanking plugboards 8-11 which are arranged at intervals in sequence are all arranged on the moving part of the blanking jacking module. The relative positions of the six blanking inserting plates 8-11 correspond to the relative positions of the six placing positions of the extraction pipe jig 9-1. The six blanking inserting plates 8-11 can extend upwards into six placing positions of the extraction tube jig 9-1 at the blanking station, and push out six nucleic acid magnetic bead extraction tubes in the extraction tube jig 9-1. The blanking jacking module comprises a blanking electric cylinder 8-12 and an electric cylinder mounting plate 8-13. The blanking electric cylinder 8-12 is arranged on the frame 10 through an electric cylinder mounting plate 8-13. The pushing rods of the blanking electric cylinders 8-12 are arranged and fixed with the blanking plugboards 8-11 through connecting frames.

The blanking transfer assembly comprises blanking clamping jaw groups 8-9 and a blanking moving module. The blanking clamping jaw groups 8-9 are arranged on the tail end mounting block of the blanking moving module. The blanking moving module is used for driving the blanking clamping jaw groups 8-9 to perform lifting and transverse movement and rotate around a vertical axis, and can drive the blanking clamping jaw groups 8-9 to move to the position right above a blanking station and the position right above the blanking conveying assembly. The structure of the blanking clamping jaw group 8-9 is the same as that of the feeding clamping jaw group 1-5, and six nucleic acid magnetic bead extraction tubes 11 on the extraction tube jig 9-1 can be clamped simultaneously.

The blanking moving module comprises a discharging mounting bottom plate 8-2, a discharging support column 8-3, a discharging mounting side plate 8-7, a blanking transverse moving module, a blanking lifting module 8-8 and a rotary cylinder. The bottom end of the discharging pillar 8-3 is fixed on the frame 10 through the discharging mounting bottom plate 8-2. The discharging installation side plate 8-7 is fixed on the discharging support column 8-3 and is adjustable in height. The blanking transverse moving module is arranged on the discharging installation side plate 8-7. The blanking lifting module 8-8 is arranged on the moving part of the blanking transverse moving module. The rotary cylinder is arranged on the moving part of the blanking lifting module 8-8. The rotating part of the rotating cylinder faces to the right lower part, and the rotating axis is vertically arranged; the blanking clamping jaw groups 8-9 are arranged on the rotating part of the rotating cylinder.

The blanking conveying assembly comprises an outer conveyor support 8-1, an inner conveyor support 8-4, a conveyor 8-5 and a conveying material baffle 8-6. The outer support 8-1 and the inner support 8-4 of the conveyor are both fixed with the frame. The conveyor 8-5 is arranged on the outer support 8-1 of the conveyor and the inner support 8-4 of the conveyor. The conveyor 8-5 adopts a belt conveyor. The U-shaped conveying material baffle 8-6 is installed at the input end of the conveyor 8-5 and used for preventing the nucleic acid magnetic bead extraction tube 11 from falling out of the conveyor 8-5 when the nucleic acid magnetic bead extraction tube 11 is placed on the conveyor 8-5 by the blanking transfer assembly.

step one, a vibration disc 1-1 in a feeding mechanism 1 works to convey a nucleic acid magnetic bead extraction tube 11 to a material level to be taken. The grabbing component 1-2 grabs the nucleic acid magnetic bead extraction tube 11 to the first storage platform 1-3-7.

When the six storage positions in the first storage table 1-3-7 are fully filled, the synchronous wheels 1-3-11 rotate under the power of the motors, the first storage table 1-3-7 moves rightwards under the guidance of the linear guide rails 1-3-9, and the second storage table 1-3-10 moves leftwards due to the fact that the synchronous belts are connected with two stations at the same time. Meanwhile, the first roller 1-3-4 is guided by the cam groove 1-3-2 to pull the lifting frame 1-3-5 downwards, and the process is guided by the alternative guide shaft 1-3-6 sliding in the sliding plate 1-3-8, so that the first storage table 1-3-7 moves downwards stably. When the first storage table 1-3-7 is moved to the middle, it is just under the second storage table 1-3-10, so that there is no interference between the two stations. As the first roller 1-3-4 travels to the right side of the cam groove 1-3-2, the first depositing table 1-3-7 starts to ascend until it reaches the normal operation position, by the same principle.

The clamping jaw cylinder 1-6 is opened first, so that the feeding clamping jaw 1-5 is in an open state. Then the material is moved to a second storage table 1-3-10 from the material loading position under the drive of a material loading traverse manipulator 1-4. At this time, the clamping jaw cylinders 1-6 are closed, so that the feeding clamping jaws 1-5 grasp the nucleic acid magnetic bead extraction tube 11. Then the feeding traversing manipulator 1-4 is lifted, so that the nucleic acid magnetic bead extraction tube 11 is separated from the second storage table 1-3-10. And finally, the feeding transverse moving manipulator 1-4 transversely moves to a feeding position and descends, and the nucleic acid magnetic bead extraction tube 11 is placed into an extraction tube jig 9-1 of the station switching mechanism 9.

And step two, the turntable of the station switching mechanism 9 rotates under the power of a motor, so that the extraction tube jig 9-1 provided with the nucleic acid magnetic bead extraction tube 11 operates the flattening and material cleaning station.

At this time, the flattening cylinders 2 to 7 press downward to flatten the nucleic acid magnetic bead extraction tubes 11 which are not in place, and then the tubes are raised to the original positions. If the flattening cylinder 2-7 reaches the lower limit position (namely, the limit sensor detects a signal) in the process, the nucleic acid magnetic bead extraction tube 11 is flattened to the designated position, and the turntable 9-2 carries the extraction tube jig 9-1 to rotate to the magnetic bead filling station. If the lower limit sensor is not bright, the nucleic acid magnetic bead extraction tube is proved not to be pressed to a designated position, at the moment, the material cleaning lifting cylinder 2-6 extends downwards, and the material cleaning horizontal cylinder 2-4 and the material cleaning hook plate 2-9 arranged on the material cleaning lifting cylinder are descended to a working position. The jacking material cleaning cylinder 2-2 extends upwards to push the nucleic acid magnetic bead extraction tube 11 out of the extraction tube jig 9-1. At the moment, the material cleaning horizontal cylinder 2-4 contracts to drive the material cleaning hook plate 2-9 on the material cleaning horizontal cylinder to hook away the nucleic acid magnetic bead extraction tube 11, and after the nucleic acid magnetic bead extraction tube falls into a specified position, the material cleaning horizontal cylinder 2-4 extends out again, and the material cleaning lifting cylinder 2-6 contracts to enable the material cleaning hook plate 2-9 to return to a standby position. At the moment, the turntable 9-2 carries the extraction tube jig 9-1 to rotate to the magnetic bead filling station.

And step three, magnetic beads are filled in the magnetic bead barrel 3-5, and the magnetic bead barrel 3-5 is rotated to a liquid adding position by the magnetic bead barrel driving motor 3-3, so that liquid is added to the magnetic bead tank 3-2. After the liquid is added, the magnetic bead tank 3-2 moves to a liquid taking position under the driving of the magnetic bead tank transverse moving module 3-1. The liquid-transfering gun 3-6 moves horizontally with the liquid-transfering gun moving component 3-7 to the liquid-fetching position. At the moment, the transverse moving manipulator 3-7 drives the liquid-transferring gun to descend to the position below the liquid level to take liquid. After liquid is taken, the transverse moving manipulator 3-7 drives the liquid moving gun to rise to the original position, then the liquid moving gun horizontally moves to the working position, then the liquid moving gun descends into the nucleic acid magnetic bead extraction tube 11, a reagent is added into the nucleic acid magnetic bead extraction tube 11, then the liquid moving gun rises to the standby position, and the turntable 9-2 carries the extraction tube jig 9-1 to rotate to the liquid injection station.

And step four, extending the liquid injection jacking cylinder 4-2, jacking the extraction pipe placing box 9-1-3 upwards by the liquid injection jacking block 4-3 on the liquid injection jacking cylinder for a certain distance from the extraction pipe base 9-1-1 under the guidance of the linear bearing 9-1-5 to enable the extraction pipe placing box to be close to the liquid injection pipe 4-8 of the liquid injection plate 4-7, shrinking the liquid injection jacking cylinder 4-2 after the liquid injection pipe 4-8 is injected, descending the extraction pipe placing box 9-1-3 to the original position, and rotating the turntable 9-2 to a hot pressing station with the extraction pipe jig 9-1.

And step five, the aluminum film 5-24 is driven by the film feeding lifting module 5-5 to move upwards to a working position along the film side stop lever 5-1, the film feeding lifting cylinder 5-10 extends out to drive the sucker 5-23 to descend, the sucker 5-23 sucks the aluminum film at the negative pressure, and then the film feeding lifting cylinder 5-10 contracts to lift the aluminum film 5-24. At the moment, the film feeding transverse moving cylinder 5-11 transversely moves, and the aluminum film 5-24 is moved to be right above the extracting tube jig 9-1. Then the film feeding lifting cylinder 5-10 extends out to drive the aluminum film 5-24 to descend, the aluminum film is placed on the extraction tube jig 9-1, the negative pressure stops, the film feeding lifting cylinder 5-10 contracts, and the aluminum film 5-24 is separated from the sucker 5-2. At this time, the film feeding and traversing cylinder 5-11 returns to the original position. And then the hot-pressing jacking cylinder 5-22 extends out to drive the inclined wedge inserting plate 5-19 to move forward in the inserting plate guide seat 5-20, and the second roller 5-18 is lifted, and the process is guided by the hot-pressing jacking sliding rail 5-16, so that the hot-pressing jacking frame 5-14 is lifted, pressure bearing during hot pressing is ensured, and the rotary plate 9-2 cannot be bent. The hot pressing cylinder 5-8 extends out to drive the hot pressing plate 5-9 to press downwards, and the aluminum film 5-24 is bonded with the nucleic acid magnetic bead extraction tube 11. At the moment, the hot-pressing cylinder 5-8 retracts to drive the hot-pressing plate to ascend, the hot-pressing jacking cylinder 5-22 retracts to drive the inclined wedge inserting plate 5-19 to retreat in the inserting plate guide seat 5-20, and the second roller 5-18 descends under the action of gravity. Then the turntable 9-2 carries the extracting tube jig 9-1 to rotate to the cold pressing station.

And step six, the cold pressing jacking module drives the cold pressing jacking frame 6-8 to lift so as to ensure that the turntable 9-2 cannot be bent under the condition of pressure bearing during cold pressing. At the moment, the cold pressing cylinder 6-4 extends downwards to drive the cold pressing heat dissipation plate 6-2 to press the surface of the aluminum film 5-24, and the aluminum film is cooled. After the preset time, the cold-pressing cylinder 6-4 retracts upwards to enable the cold-pressing heat dissipation plate 6-2 to be separated from the aluminum film 5-24 again; and the cold pressing jacking module drives the cold pressing jacking frame 6-8 to descend. The turntable 9-2 carries the extraction tube jig 9-1 to rotate to the film cutting station.

And seventhly, the film cutting traversing cylinder 7-5 moves forwards to a first section of stroke, the film cutting lifting cylinder 7-6 extends downwards to drive the cutter 7-1 to cut into the aluminum film 5-24 downwards, the film cutting traversing cylinder 7-5 moves forwards to a second section of stroke at the moment, and then the film cutting traversing cylinder returns to the original position backwards, so that the cutter 7-1 completes front and back film cutting actions under the guidance of the film cutting traversing guide rail 7-3 along with the cutter. And finally, the film cutting lifting cylinder 7-6 retracts upwards and returns to the original position. At the moment, the turntable 9-2 carries the extraction tube jig 9-1 to rotate to a blanking station.

Step eight, the blanking electric cylinder 8-12 extends upwards to drive the blanking inserting plate 8-11 to lift upwards until the nucleic acid magnetic bead extraction pipe 11 is ejected out of the extraction pipe jig 9-1 for a certain distance, and then the blanking lifting module 8-8 drives the discharging clamping jaw 8-10 to move to the position above the nucleic acid magnetic bead extraction pipe 11, the clamping jaw air cylinder 8-9 is opened, the blanking lifting module 8-8 descends, the clamping jaw air cylinder 8-9 is closed after the blanking lifting module reaches a specified position, so that the discharging clamping jaw 8-10 clamps the nucleic acid magnetic bead extraction pipe 11, at the moment, the blanking lifting module 8-8 starts to ascend to drive the nucleic acid magnetic bead extraction pipe 11 to be separated from the extraction pipe jig 9-1, when the blanking lifting module 8-8 moves back to a certain height, the blanking lifting module reaches the position right above the conveyor 8-5 again, and descends to the specified height, the clamping jaw air cylinder 8-9 is opened, and the nucleic acid magnetic bead extraction pipe 11 falls on the upper surface of the conveyor 8-5. At the moment, the conveyor 8-5 starts to run and is conveyed out of the machine table, and thus, one-time filling and film sealing action is completed.

Step one to eight, to the synchronous operation of different extraction tube tools, can last, carry out extraction tube filling, seal membrane operation fast.

Claims

1. A full-automatic nucleic acid magnetic bead extraction tube filling and film sealing device comprises a rack (10) and a station switching mechanism (9); the method is characterized in that: the automatic film sealing and feeding device also comprises a feeding mechanism (1), a flattening and cleaning mechanism (2), a magnetic bead filling mechanism (3), a liquid injection mechanism (4), a film sealing hot-pressing mechanism (5), a cold-pressing mechanism (6), a film cutting mechanism (7) and a discharging mechanism (8) which correspond to eight stations on the station switching mechanism (9) in position respectively; the eight stations are respectively a feeding station, a flattening and cleaning station, a magnetic bead filling station, a liquid injection station, a hot pressing station, a cold pressing station, a film cutting station and a discharging station;

the station switching mechanism (9) comprises a turntable (9-2), an extraction tube jig (9-1) and a driving mechanism; the horizontally arranged rotary table (9-2) is rotationally connected to the rack (10) and is driven by the driving mechanism to rotate; eight extraction pipe jigs (9-1) are arranged on the rotary table (9-2); the rotation of the turntable (9-2) drives each extraction pipe jig (9-1) to sequentially pass through eight stations; the extraction tube jig (9-1) is provided with a plurality of placing positions; each placing position can be used for placing a nucleic acid magnetic bead extraction tube (11); the bottoms of the placing positions are hollowed out;

the feeding mechanism (1) is used for placing a nucleic acid magnetic bead extraction tube (11) on an extraction tube jig (9-1) of a feeding station;

the flattening and material clearing mechanism (2) comprises a flattening component and a material clearing component; the flattening component is used for pushing and flattening the extraction tube jig (9-1) on the material clearing station downwards so that each nucleic acid magnetic bead extraction tube (11) is placed on the extraction tube jig (9-1) to be flattened; the material cleaning component is used for pushing the nucleic acid magnetic bead extraction tube (11) out of the extraction tube jig (9-1) in a mode that the material cleaning inserting plate (2-3) is upwards inserted into the placing position of the extraction tube jig (9-1);

the magnetic bead filling mechanism (3) is used for adding magnetic beads into the nucleic acid magnetic bead extraction tube (11); the liquid injection mechanism (4) is used for adding a reagent into the nucleic acid magnetic bead extraction tube (11);

the film sealing hot-pressing mechanism (5) is used for sticking the aluminum film on each nucleic acid magnetic bead extraction tube (11) of the hot-pressing station in a hot-pressing mode;

the cold pressing mechanism (6) is used for extruding and cooling the aluminum film on the cold pressing station;

the membrane cutting mechanism (7) is used for cutting the aluminum membranes positioned on the membrane cutting station, so that the aluminum membranes on the nucleic acid magnetic bead extraction tubes (11) are mutually independent;

the blanking mechanism (8) is used for taking out each nucleic acid magnetic bead extraction tube (11) in the extraction tube jig (9-1) of the blanking station.

2. The full-automatic nucleic acid magnetic bead extraction tube filling and membrane sealing device according to claim 1, characterized in that: the extraction pipe jig (9-1) comprises a jig base and a jig lifting platform; the jig lifting table is connected to the jig base in a sliding manner; the jig base comprises an extraction tube base (9-1-1), a film cutting guide plate (9-1-2) and a jig guide shaft (9-1-4); the extraction tube base (9-1-1) is fixed on the turntable; a plurality of vertically arranged jig guide shafts (9-1-4) are fixed at the top of the extraction tube base (9-1-1); the film cutting guide plate (9-1-2) is fixed with the top end of each jig guide shaft (9-1-4); n through grooves are arranged on the film cutting guide plate (9-1-2) at equal intervals in sequence; n is the number of placing positions on the extracting tube jig (9-1); a cutter abdicating groove is formed between any two of the placing through grooves; the jig lifting platform comprises an extraction tube placing box (9-1-3); the extraction tube placing box (9-1-3) is connected to the jig guide shaft (9-1-4) in a sliding manner; the placing position is arranged on the extracting tube placing box (9-1-3); the n placing through grooves are aligned with the n placing positions respectively.

3. The full-automatic nucleic acid magnetic bead extraction tube filling and membrane sealing device according to claim 1, characterized in that: the feeding mechanism (1) comprises a preposed integration component and an extraction pipe transfer component; the number of the preposed integration components is two; the two preposed integration components are used for arranging the stacked nucleic acid magnetic bead extraction tubes (11) into n extraction tube arrays in one group; n is the number of placing positions on the extracting tube jig (9-1); the extraction pipe transfer component is used for conveying the extraction pipe arrays arranged by the two preposed integration components to an extraction pipe jig (9-1) of the feeding station;

the preposed integration component comprises a vibration disc (1-1), a grabbing component (1-2) and a transverse movement alternating interchanging component (1-3); the vibrating disc (1-1) is arranged on the frame and used for arranging and outputting the stacked nucleic acid magnetic bead extraction tubes one by one; the grabbing component is used for grabbing the nucleic acid magnetic bead extraction tubes one by one onto a storage table of the transverse moving alternate exchange component (1-3);

the transverse moving alternate interchange assembly (1-3) comprises a transverse moving base (1-3-1), a cam groove (1-3-2), a synchronous belt (1-3-3), a first roller (1-3-4), a lifting frame (1-3-5), an alternate guide shaft (1-3-6), a first storage table (1-3-7), a sliding plate (1-3-8), a linear guide rail (1-3-9), a second storage table (1-3-10) and a synchronous wheel (1-3-11); the transverse moving base (1-3-1) is fixed on the rack; a lifting guide plate is fixed on the transverse moving base (1-3-1); the lifting guide plate is provided with a cam groove (1-3-2); four synchronizing wheels (1-3-11) which are arranged in a rectangular shape in a vertical plane are rotatably connected to the lifting guide plate; the four synchronous wheels (1-3-11) are in transmission connection through the synchronous belts (1-3-3); one of the synchronous wheels (1-3-11) is driven by a motor; the second storage table (1-3-10) and the sliding plate (1-3-8) are connected to the transverse moving base (1-3-1) in a sliding manner through a sliding block; the sliding plate (1-3-8) is lower than the second storage table (1-3-10);

the lifting frame (1-3-5) and the sliding plate (1-3-8) form a sliding pair which slides along the vertical direction; the bottom of the lifting frame (1-3-5) is rotationally connected with a first roller (1-3-4); the first roller (1-3-4) is arranged in the cam groove (1-3-2); a first storage table (1-3-7) is fixed at the top of the lifting frame (1-3-5); the first storage table (1-3-7) and the second storage table (1-3-10) are respectively provided with n storage positions; the relative position of each storage position corresponds to the relative position of each placing position on the extraction pipe fixture respectively;

the cam groove (1-3-2) is in a shape with two high ends and a low middle part; when the first roller (1-3-4) is positioned at the highest position of the end part of the cam groove (1-3-2), the first storage table (1-3-7) and the second storage table (1-3-10) are equal in height; when the first roller (1-3-4) is at the lowest position in the middle of the cam groove (1-3-2), the top of the first storage table (1-3-7) is lower than the bottom of the second storage table (1-3-10), and the first storage table (1-3-7) can pass right below the second storage table (1-3-10); the sliding plate (1-3-8), the second storage table (1-3-10) and the two horizontal sections of the synchronous belt are respectively fixed; when the synchronous belt moves, the sliding plate (1-3-8) and the second storage table (1-3-10) move reversely at a constant speed to drive the first storage table (1-3-7) and the second storage table (1-3-10) to exchange positions;

the extraction pipe transfer assembly comprises an extraction pipe moving module and a feeding clamping jaw group (1-5); a feeding clamping jaw set (1-5) is arranged on the tail end mounting block of the extraction pipe moving module; the feeding clamping jaw group (1-5) comprises n mechanical clamping jaws which are sequentially arranged; the mechanical clamping jaw is used for grabbing a single nucleic acid magnetic bead extraction tube; the extraction tube moving module is used for driving the feeding clamping jaw set (1-5) to move to the position right above the first storage platform (1-3-7) or the second storage platform (1-3-10) and the position right above the feeding station.

4. The full-automatic nucleic acid magnetic bead extraction tube filling and membrane sealing device according to claim 1, characterized in that: the flattening assembly comprises a flattening cylinder (2-7) and a pressing plate (2-8); the flattening cylinder (2-7) is fixed on the frame; a pressing plate (2-8) is fixed on the downward push-out rod of the flattening cylinder (2-7); the pressing plate (2-8) is positioned right above the flattening and material cleaning station;

the material cleaning component comprises a jacking material cleaning cylinder (2-2), a material cleaning inserting plate (2-3), a material cleaning moving module, a material cleaning hook plate (2-9) and a waste material tank body (2-10); the waste material tank body (2-10) is fixed on the frame and is close to the flattening and material cleaning station; the jacking material cleaning cylinder (2-2) is fixed on the frame and is positioned right below the flattening material cleaning station; n material cleaning inserting plates (2-3) which are arranged at intervals in sequence are fixed on an upward pushing rod of the jacking material cleaning cylinder (2-2); n is the number of placing positions on the extracting tube jig (9-1); the relative positions of the n material cleaning inserting plates (2-3) correspond to the relative positions of the n placing positions of the extraction pipe jig (9-1);

the material cleaning moving module is arranged on the rack; the material cleaning hook plate (2-9) is arranged on a moving part of the material cleaning moving module; the bottom surface of the material cleaning hook plate (2-9) is provided with a hook groove; the material cleaning hook plate (2-9) can convey the nucleic acid magnetic bead extraction tube (11) pushed out of the extraction tube jig (9-1) into the waste material tank body (2-10) under the driving of the material cleaning moving module.

5. The full-automatic nucleic acid magnetic bead extraction tube filling and membrane sealing device according to claim 2, characterized in that: the magnetic bead filling mechanism (3) comprises a magnetic bead groove transverse moving module (3-1), a magnetic bead groove (3-2), a magnetic bead barrel driving motor (3-3), a magnetic bead barrel mounting seat (3-4), a magnetic bead barrel (3-5), a liquid transferring gun (3-6) and a liquid transferring gun moving assembly (3-7); the liquid-transfering guns (3-6) of the n channels are arranged on the moving part of the liquid-transfering gun moving assembly (3-7); n is the number of placing positions on the extracting tube jig (9-1); the relative positions of n output ports of the liquid-transferring gun (3-6) correspond to the relative positions of n placing positions of the extracting tube jig (9-1); the pipetting gun moving assembly (3-7) can move the pipetting gun (3-6) to a position right above the magnetic bead tank and the magnetic bead filling station;

the magnetic bead barrel mounting seat (3-4) is rotationally connected to the rack (10) and is driven to turn over by the magnetic bead barrel driving motor (3-3); the magnetic bead barrel (3-5) is arranged on the magnetic bead barrel mounting seat (3-4); an electromagnetic valve is arranged at the output port of the magnetic bead barrel (3-5); the magnetic bead groove (3-2) is arranged on the rack (10) through the magnetic bead groove transverse moving module (3-1); the magnetic bead groove transverse moving module (3-1) can drive the magnetic bead groove (3-2) to switch between a magnetic bead pouring position and a magnetic bead sucking position; the magnetic bead pouring position of the magnetic bead groove (3-2) corresponds to the outlet position of the magnetic bead barrel (3-5); the magnetic bead sucking position of the magnetic bead groove (3-2) corresponds to the position of the liquid transferring gun (3-6);

the liquid injection mechanism (4) comprises a jacking mounting seat (4-1), a liquid injection jacking cylinder (4-2), a liquid injection jacking block (4-3), a liquid receiving groove (4-4), a liquid injection transverse moving mounting seat (4-5), a liquid injection transverse moving cylinder (4-6), a liquid injection plate (4-7) and a liquid injection pipe (4-8); the input port of the liquid injection plate (4-7) is connected to a liquid source through a liquid injection pipe (4-8); the liquid receiving tank (4-4) is arranged on the frame; a horizontally arranged liquid injection transverse cylinder (4-6) is arranged on the rack (10); the liquid injection plate (4-7) is arranged on a pushing rod of the liquid injection transverse cylinder (4-6) and fixed; the liquid injection transverse cylinder (4-6) can drive the liquid injection plate (4-7) to be switched between the position right above the liquid injection station and the position right above the liquid receiving groove (4-4); the liquid injection jacking cylinder (4-2) is installed on the rack (10) through a jacking installation seat (4-1); a liquid injection jacking block (4-3) is fixed on the upward pushing rod of the liquid injection jacking cylinder (4-2); the liquid injection jacking block (4-3) is used for jacking up each nucleic acid magnetic bead extraction tube (11) in the extraction tube jig (9-1) so that the nucleic acid magnetic bead extraction tube (11) is close to the liquid outlet of the liquid injection plate (4-7).

6. The full-automatic nucleic acid magnetic bead extraction tube filling and sealing membrane equipment of claim 2, characterized in that: the film sealing hot-pressing mechanism (5) comprises a film supply assembly, a film transfer assembly and a hot-pressing assembly; the film supply assembly comprises a film side stop lever (5-1), a film supply mounting seat (5-4), a film feeding lifting module (5-5) and a film stacking support (5-6); a plurality of membrane side stop rods (5-1) which are vertically arranged are all fixed on the frame (10); each diaphragm side stop lever (5-1) surrounds a rectangular diaphragm limiting area; the film feeding lifting module (5-5) is arranged on the frame (10); a membrane stacking bracket (5-6) is arranged on the moving part of the membrane conveying lifting module (5-5); the membrane stacking support (5-6) is positioned in the membrane limiting area and used for supporting the stacked aluminum membrane;

the membrane transfer assembly comprises a membrane moving module, a sucker mounting plate (5-13) and a sucker (5-23); the sucker mounting plates (5-13) are mounted on the moving part of the diaphragm moving module; a plurality of suckers (5-23) arranged downwards are all arranged on the sucker mounting plates (5-13); the membrane moving module can drive the sucker mounting plate (5-13) to move to the position right above the membrane stacking support (5-6) and the position right above the hot pressing station;

the hot pressing assembly comprises a hot pressing plate (5-9), a hot pressing lifting module, a hot pressing jacking frame (5-14) and a hot pressing jacking module; the hot-pressing lifting module is arranged on the frame (10); the hot-pressing jacking module is arranged on the rack; the hot pressing plates (5-9) are arranged on the moving part of the hot pressing lifting module; heating elements are arranged in the hot pressing plates (5-9); the hot-pressing jacking frame (5-14) is arranged on the moving part of the hot-pressing jacking module; the hot pressing plate (5-9) is positioned right above the hot pressing station; the hot-pressing jacking frames (5-14) are positioned right below the hot-pressing station; in the working process, the hot-pressing jacking frame (5-14) is lifted to support the extraction pipe jig (9-1) on the hot-pressing station; the hot pressing plates (5-9) start to heat and move downwards, and the aluminum film is pressed to be bonded with the openings of the nucleic acid magnetic bead extraction tubes (11).

7. The full-automatic nucleic acid magnetic bead extraction tube filling and membrane sealing device according to claim 2, characterized in that: the cold pressing mechanism (6) comprises a cold pressing plate (6-1), a cold pressing heat dissipation plate (6-2), a cold pressing fan (6-3), a cold pressing mounting bracket, a cold pressing lifting module, a cold pressing jacking frame (6-8) and a cold pressing jacking module; the cold pressing lifting module and the cold pressing jacking module are both arranged on the rack; the cold pressing lifting module is arranged on the moving part of the cold pressing lifting module; a cold pressing plate (6-1) is fixed on the bottom surface of the cold pressing heat dissipation plate (6-2), and a cold pressing fan (6-3) is installed on the top surface; the cold pressing jacking frame (6-8) is arranged on the moving part of the cold pressing jacking module; the cold pressing plate (6-1) is positioned right above the cold pressing station; the cold pressing jacking frame (6-8) is positioned right below the cold pressing station; in the working process, the cold pressing jacking frame (6-8) is lifted to support the extraction pipe jig (9-1) on the cold pressing station; the cold-pressing plate (6-1) moves downwards to press the aluminum film adhered on each nucleic acid magnetic bead extraction tube (11) and reduce the temperature of the aluminum film.

8. The full-automatic nucleic acid magnetic bead extraction tube filling and membrane sealing device according to claim 1, characterized in that: the film cutting mechanism (7) comprises a cutter (7-1), a cutter mounting seat (7-2) and a film cutting moving module; the cutter mounting seat (7-2) is arranged on the moving part of the film cutting moving module; n-1 cutters (7-1) are fixed on the cutter mounting seat (7-2); n is the number of placing positions on the extracting tube jig (9-1); the relative position of each cutter (7-1) corresponds to the gap position between each placing position on the extraction pipe jig (9-1); the film cutting moving module can drive the cutter to lift and transversely move; the cutting edge of the cutter (7-1) is in a downward convex V shape, and the bottom of the cutter is provided with a sharp point; in the working process, the V-shaped cutting edge of the cutter (7-1) is firstly cut into the middle of the aluminum film and then moves towards the two side edges of the aluminum film in sequence to cut off the aluminum film.

9. The full-automatic nucleic acid magnetic bead extraction tube filling and membrane sealing device according to claim 1, characterized in that: the blanking mechanism (8) comprises a blanking jacking assembly, a blanking transfer assembly and a blanking conveying assembly; the blanking jacking assembly comprises a blanking plugboard (8-11) and a blanking jacking module; n blanking plugboards (8-11) which are sequentially arranged at intervals are all arranged on the moving part of the blanking jacking module; n is the number of placing positions on the extracting tube jig (9-1); the relative positions of the n blanking inserting plates (8-11) correspond to the relative positions of the n placing positions of the extraction pipe jig (9-1); the n blanking inserting plates (8-11) can extend upwards into n placing positions of the extraction tube jig (9-1) at the blanking station to eject the n nucleic acid magnetic bead extraction tubes in the extraction tube jig (9-1);

the blanking transfer component comprises a blanking clamping jaw group (8-9) and a blanking moving module; the blanking clamping jaw group (8-9) is arranged on the tail end mounting block of the blanking moving module; the blanking moving module can drive the blanking clamping jaw group (8-9) to perform lifting and transverse movement and rotate around a vertical axis, and can drive the blanking clamping jaw group (8-9) to move to the position right above a blanking station and the position right above the blanking conveying assembly; the blanking clamping jaw group (8-9) can simultaneously clamp n nucleic acid magnetic bead extraction tubes (11) on the extraction tube jig (9-1);

the blanking conveying assembly comprises a conveyor (8-5) and a conveying material baffle plate (8-6); the conveyor (8-5) is arranged on the frame; the input end of the conveyor (8-5) is provided with a U-shaped conveying striker plate (8-6).

10. A full-automatic nucleic acid magnetic bead extraction tube filling and membrane sealing method is characterized in that: filling and sealing membrane equipment by using the full-automatic nucleic acid magnetic bead extraction tube according to any one of claims 1 to 9; the method comprises the following steps:

step one, a feeding mechanism (1) puts a nucleic acid magnetic bead extraction tube (11) into an extraction tube jig (9-1) of a feeding station;

rotating the turntable (9-2), and transferring the extraction pipe jig (9-1) of the feeding station to a flattening and material cleaning station; a flattening component of the flattening and material cleaning mechanism (2) pushes and presses each nucleic acid magnetic bead extraction tube (11) in the extraction tube jig (9-1) downwards; if the nucleic acid magnetic bead extraction pipes cannot be pushed to the right position, the cleaning component removes the nucleic acid magnetic bead extraction pipes (11) from the pushing extraction pipe jig (9-1);

rotating the turntable (9-2), and transferring the extraction tube jig (9-1) of the flattening and material cleaning station to a magnetic bead filling station; the magnetic bead filling mechanism (3) fills magnetic beads into the nucleic acid magnetic bead extraction tube (11);

rotating the turntable (9-2), and transferring the extraction tube jig (9-1) of the magnetic bead filling station to the liquid injection station; the liquid injection mechanism (4) injects a reagent into the nucleic acid magnetic bead extraction tube (11);

rotating the turntable (9-2), and transferring the extraction tube jig (9-1) of the liquid injection station to a hot pressing station; an aluminum film is placed on each nucleic acid magnetic bead extraction tube (11) by the film sealing hot-pressing mechanism (5), and the aluminum film is adhered to the opening of the nucleic acid magnetic bead extraction tube (11) in a hot-pressing mode;

sixthly, rotating the turntable (9-2), and transferring the extraction pipe jig (9-1) of the hot pressing station to the cold pressing station; the cold pressing mechanism (6) extrudes the aluminum film, and reduces the temperature of the aluminum film through heat transfer;

seventhly, rotating the turntable (9-2), and transferring the extraction pipe jig (9-1) of the cold pressing station to a film cutting station; the membrane cutting mechanism (7) cuts the aluminum membrane of the nucleic acid magnetic bead extraction tube (11) between every two membrane cutting mechanisms;

step eight, rotating the turntable (9-2) to transfer the extraction tube jig (9-1) of the film cutting station to a blanking station; the blanking mechanism (8) takes out the nucleic acid magnetic bead extraction tubes (11) in the extraction tube jig (9-1).