CN115443758A - Method for promoting germination of rhizoma atractylodis macrocephalae seeds - Google Patents
Method for promoting germination of rhizoma atractylodis macrocephalae seeds Download PDFInfo
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- CN115443758A CN115443758A CN202211339619.6A CN202211339619A CN115443758A CN 115443758 A CN115443758 A CN 115443758A CN 202211339619 A CN202211339619 A CN 202211339619A CN 115443758 A CN115443758 A CN 115443758A
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- seeds
- rhizoma atractylodis
- atractylodis macrocephalae
- seed
- promoting germination
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
Abstract
The invention discloses a method for promoting germination of rhizoma atractylodis macrocephalae seeds, and relates to the technical field of seed treatment. According to the method, the selected rhizoma atractylodis macrocephalae seeds are disinfected, seed coating agent containing bactericide, insecticide and composite plant regulator is adopted for seed soaking, and finally the soaked rhizoma atractylodis macrocephalae seeds are wrapped by the film coating agent, so that the germination rate of the rhizoma atractylodis macrocephalae seeds is remarkably improved. The treatment method of the invention adopts a film coating method to ensure that the size of the seeds is not changed and the seeds absorb the active ingredients in the seed coating agent, thereby improving the germination rate of the seeds, effectively shortening the seedling emergence time of the seeds and reducing the planting cost of the Atractylodes lancea.
Description
Technical Field
The invention belongs to the technical field of seed treatment, and particularly relates to a method for promoting germination of seeds of Atractylodes lancea.
Background
Rhizoma Atractylodis is perennial herb of Atractylodes of Compositae, and has effects of eliminating dampness, invigorating spleen, dispelling pathogenic wind, dispelling cold, resisting inflammation, and resisting lung injury. The problems that wild resources of the rhizoma atractylodis sinensis are gradually exhausted, the wild species are bred, the wild species are difficult to domesticate, the artificial cultivation technology is immature, the growth period of the rhizoma atractylodis sinensis is long and the like cause short supply of the rhizoma atractylodis in the market, and the research on artificial propagation of the rhizoma atractylodis sinensis and improvement of the yield of the rhizoma atractylodis sinensis are hot spots of the current research.
The artificial propagation mode of the rhizoma atractylodis macrocephalae mainly comprises tuber propagation and seed propagation, wherein the requirements of the tissue culture technology in the rhizome propagation of the rhizoma atractylodis macrocephalae on aseptic conditions are strict and difficult to master, so that the propagation method of the rhizoma atractylodis macrocephalae seeds is imperative. The seeds of the rhizoma atractylodis macrocephalae are short-lived seeds, the seeds lose life vitality in half a year at normal temperature, and the seed vitality is greatly influenced by environmental changes and is not easy to store. Meanwhile, the problems of low germination rate, slow seedling emergence and the like exist in the propagation and seedling process of the seeds of the Atractylodes chinensis, so that the key for improving the yield of the Atractylodes chinensis is to solve the germination problem of the seeds of the Atractylodes chinensis.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a method for promoting the germination of seeds of Atractylodes chinensis, which is to treat the seeds of Atractylodes chinensis before sowing so as to improve the germination rate and emergence rate of the seeds, effectively shorten the emergence time of the seeds, reduce the planting cost of Atractylodes chinensis and ensure the uniform and strong seedlings.
The invention is realized by adopting the following technical scheme:
a method for promoting germination of rhizoma atractylodis macrocephalae seeds comprises the following steps:
(1) Seed selection: selecting seeds of the rhizoma atractylodis macrocephalae which have no plant diseases and insect pests and are full in grains;
(2) Seed disinfection: disinfecting the selected rhizoma atractylodis macrocephalae seeds by adopting a sodium hypochlorite aqueous solution;
(3) Preparing a seed treating agent:
1L of the seed treatment agent contains 1-2 mL of bactericide, 1-5 mL of insecticide, 100-200 mg of plant regulator and the balance of water; the plant growth regulator is prepared from 6-BA and GA 3 Forming;
(4) Seed soaking: soaking the disinfected rhizoma atractylodis macrocephalae seeds in the seed treatment solution for 6-8h, taking out after soaking, and airing;
(5) Coating: wrapping the air-dried Atractylodes lancea seeds according to the mass to volume ratio of the coating agent of 1-2:1.
Preferably, the thousand seed weight selected in step (1) is more than 10g.
Preferably, the mass concentration of the sodium hypochlorite aqueous solution in the step (2) is 2%.
Preferably, the disinfection time in step (2) is 15min.
Preferably, the bactericide in the step (3) is 800 times of thiophanate methyl solution.
Preferably, the pesticide in the step (3) is imidacloprid 500-fold liquid.
Preferably, the 6-BA (6-benzylamino adenine) and GA described in step (3) 3 The mass ratio of (gibberellin) was 2:1.
Preferably, the coating agent in the step (4) is a chitosan solution with the mass concentration of 1-3%.
Further preferably, the chitosan solution in step (4) is prepared by dissolving chitosan in an acetic acid aqueous solution.
More preferably, the mass concentration of the acetic acid aqueous solution in the step (4) is 0.1%.
Compared with the prior art, the invention has the following beneficial effects:
according to the method, the seeds of the Atractylodes chinensis are treated before sowing, so that the germination rate of the seeds can be obviously improved, the germination time is shortened, the growth vigor of the seeds and seedlings is improved, the seeds and the seedlings are saved, the pesticide is saved, and the planting cost is saved.
Drawings
FIG. 1 shows germination of seeds of Atractylodes lancea after treatment in example 1 and comparative example 1 at low temperature;
FIG. 2 shows germination of seeds of Atractylodes lancea at a higher temperature (25-40 ℃) after treatment in example 1 and comparative example 1.
Detailed Description
In order to make the objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. Several embodiments of the invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Example 1
A method for promoting germination of rhizoma atractylodis macrocephalae seeds comprises the following specific steps:
(1) Seed selection: selecting seeds of the rhizoma atractylodis macrocephalae which have no plant diseases and insect pests and are full in grains, wherein the thousand seed weight of the selected seeds is more than 10g;
(2) Seed disinfection: soaking and disinfecting the selected rhizoma atractylodis macrocephalae seeds for 15min by adopting a sodium hypochlorite aqueous solution with the mass concentration of 2%, and then cleaning for 3 times by using clear water;
(3) Preparing a seed treating agent:
1L of the seed treatment agent contains 1.25mL of thiophanate methyl 800-fold liquid, 2mL of imidacloprid 500-fold liquid, 100mg of 6-BA and GA 3 50mg, the balance being water;
(4) Seed soaking: soaking the disinfected rhizoma atractylodis macrocephalae seeds in the seed treatment solution for 8 hours at room temperature, taking out after soaking, and airing;
(5) Coating: and (3) wrapping the aired rhizoma atractylodis macrocephalae seeds according to the ratio of the mass to the volume of the coating agent of 1.5. Wherein the coating agent is chitosan solution with mass concentration of 2%, and the preparation method is that 5g of chitosan is dissolved in 250mL of 1wt% acetic acid aqueous solution.
Comparative example 1
The sterilized seeds of example 1 were soaked in clear water without coating agent.
The seeds of atractylis ovata treated in example 1 and comparative example 1 (control group) were subjected to germination treatment and the germination rate was recorded. The specific test is as follows:
the seeds treated in example 1 and the control group were placed in a petri dish with wetted vermiculite, labeled, and incubated in incubators at 4, 10, 25, 30, and 40 ℃ respectively. Each treatment was repeated three times
After the seeds were cultured in the light incubator, the petri dish was taken out every day at 10 a.m., water was replenished in a clean bench, and the germination of the seeds was observed and recorded. Recording started on day one and ended on day seven.
The embryonic root breaks through the seed coat and grows to 2mm as the standard of germination.
FIG. 1 shows the germination conditions of seeds treated in example 1 and comparative example 1 at low temperature (4 deg.C, 10 deg.C), and it can be seen from FIG. 1 that the seeds of Atractylodes lancea treated by the present invention have high germination rate at low temperature.
FIG. 2 shows the germination conditions of the seeds treated in example 1 and comparative example 2 at higher temperature (25 deg.C, 30 deg.C, 40 deg.C), and it can be seen from FIG. 2 that the germination rate of the seeds of Atractylodes lancea treated by the present invention at higher temperature is also significantly higher than that of the control group.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A method for promoting germination of seeds of Atractylodes chinensis is characterized by comprising the following steps:
(1) Seed selection: selecting seeds of the rhizoma atractylodis macrocephalae which have no plant diseases and insect pests and are full in grains;
(2) Seed disinfection: disinfecting the selected rhizoma atractylodis macrocephalae seeds by adopting a sodium hypochlorite aqueous solution;
(3) Preparing a seed treating agent:
1L of the seed treatment agent contains 1-2 mL of bactericide, 1-5 mL of insecticide, 100-200 mg of plant growth regulator and the balance of water; the plant growth regulator is prepared from 6-BA and GA 3 Forming;
(4) Seed soaking: soaking the disinfected rhizoma atractylodis macrocephalae seeds in the seed treatment solution for 6-8h, taking out after soaking, and airing;
(5) Coating: and (3) coating the dried seeds of the Atractylodes lancea with a coating agent volume ratio of 1-2:1.
2. The method for promoting germination of seeds of Atractylodes chinensis as claimed in claim 1, wherein the seed weight of the seeds selected in step (1) is more than 10g.
3. The method for promoting germination of seeds of Atractylodes chinensis as claimed in claim 1, wherein the mass concentration of the sodium hypochlorite aqueous solution in step (2) is 2%.
4. The method for promoting germination of seeds of Atractylodes chinensis as claimed in claim 3, wherein the disinfection time in step (2) is 15min.
5. The method for promoting germination of seeds of Atractylodes chinensis as claimed in claim 1, wherein the bactericide in step (3) is 800 times of thiophanate methyl solution.
6. The method for promoting germination of seeds of rhizoma atractylodis macrocephalae according to claim 1, wherein the pesticide in the step (3) is imidacloprid 500-fold liquid.
7. The method for promoting germination of seeds of Atractylodes chinensis as claimed in claim 1, wherein 6-BA and GA are added in step (3) 3 The mass ratio was 2:1.
8. The method for promoting germination of rhizoma atractylodis macrocephalae seeds according to claim 1, wherein the coating agent in the step (4) is a chitosan solution with the mass concentration of 1-3%.
9. The method for promoting germination of rhizoma atractylodis macrocephalae seeds as claimed in claim 8, wherein the chitosan solution in step (4) is prepared by dissolving chitosan in an aqueous solution of acetic acid.
10. The method for promoting germination of seeds of rhizoma atractylodis macrocephalae according to claim 8, wherein the mass concentration of the acetic acid aqueous solution in the step (4) is 0.1%.
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| CN202211339619.6A CN115443758A (en) | 2022-10-28 | 2022-10-28 | Method for promoting germination of rhizoma atractylodis macrocephalae seeds |
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| CN202211339619.6A CN115443758A (en) | 2022-10-28 | 2022-10-28 | Method for promoting germination of rhizoma atractylodis macrocephalae seeds |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105237209A (en) * | 2015-10-20 | 2016-01-13 | 颈复康药业集团有限公司 | Seed coating agent and preparation method and application thereof |
| CN107235786A (en) * | 2017-07-28 | 2017-10-10 | 镇江绿健农业科技开发有限公司 | A kind of Atractylis lancea seed coat agent and preparation method thereof |
| CN109362515A (en) * | 2018-09-10 | 2019-02-22 | 陕西师范大学 | A kind of method of Atractylis chinensis seed seedling-raising |
| CN111386979A (en) * | 2020-03-26 | 2020-07-10 | 陕西锶长久中药材种植科技有限公司 | Seedling growing method of rhizoma atractylodis macrocephalae |
| CN113273465A (en) * | 2021-05-21 | 2021-08-20 | 国药集团承德药材有限公司 | Rapid seedling growing method for rhizoma atractylodis and application thereof |
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- 2022-10-28 CN CN202211339619.6A patent/CN115443758A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105237209A (en) * | 2015-10-20 | 2016-01-13 | 颈复康药业集团有限公司 | Seed coating agent and preparation method and application thereof |
| CN107235786A (en) * | 2017-07-28 | 2017-10-10 | 镇江绿健农业科技开发有限公司 | A kind of Atractylis lancea seed coat agent and preparation method thereof |
| CN109362515A (en) * | 2018-09-10 | 2019-02-22 | 陕西师范大学 | A kind of method of Atractylis chinensis seed seedling-raising |
| CN111386979A (en) * | 2020-03-26 | 2020-07-10 | 陕西锶长久中药材种植科技有限公司 | Seedling growing method of rhizoma atractylodis macrocephalae |
| CN113273465A (en) * | 2021-05-21 | 2021-08-20 | 国药集团承德药材有限公司 | Rapid seedling growing method for rhizoma atractylodis and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 姜雨昕等: "北苍术种子的萌发特性及其生态适应性研究", 《种子》, vol. 39, no. 8, pages 158 - 163 * |
| 曾聪明;王海斌;吴良展;林银英;蔡志祥;郭万财;严琳;何海斌;: "壳聚糖包衣水稻种子对水稻苗期生长发育的影响", 现代农业科技, no. 24, pages 117 - 118 * |
| 苏国林等: "北苍术种子包衣直播栽培方法研究", 《亚太传统医药》, vol. 12, no. 2, pages 30 - 31 * |
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