CN115433769A - 血浆dna的单分子测序 - Google Patents

血浆dna的单分子测序 Download PDF

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Publication number
CN115433769A
CN115433769A CN202211147693.8A CN202211147693A CN115433769A CN 115433769 A CN115433769 A CN 115433769A CN 202211147693 A CN202211147693 A CN 202211147693A CN 115433769 A CN115433769 A CN 115433769A
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dna
sequencing
dna fragments
nanopore
sequence
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Chinese (zh)
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卢煜明
赵慧君
郑淑恒
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Chinese University of Hong Kong CUHK
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Chinese University of Hong Kong CUHK
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/151—Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/197—Modifications characterised by incorporating a spacer/coupling moiety
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search

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  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
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  • Bioinformatics & Computational Biology (AREA)
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  • Theoretical Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nanotechnology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
CN202211147693.8A 2015-08-12 2016-08-12 血浆dna的单分子测序 Pending CN115433769A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201562204396P 2015-08-12 2015-08-12
US62/204,396 2015-08-12
PCT/CN2016/094802 WO2017025059A1 (en) 2015-08-12 2016-08-12 Single-molecule sequencing of plasma dna
CN201680046621.0A CN107849607B (zh) 2015-08-12 2016-08-12 血浆dna的单分子测序

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CN201680046621.0A Active CN107849607B (zh) 2015-08-12 2016-08-12 血浆dna的单分子测序

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US (2) US11319586B2 (de)
EP (1) EP3334843A4 (de)
JP (2) JP6867045B2 (de)
CN (2) CN115433769A (de)
AU (3) AU2016305103C1 (de)
CA (1) CA2995422A1 (de)
HK (2) HK1245850A1 (de)
IL (3) IL285795B (de)
MY (1) MY188348A (de)
SA (1) SA518390907B1 (de)
TW (2) TWI728994B (de)
WO (1) WO2017025059A1 (de)

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CN111527044A (zh) 2017-10-26 2020-08-11 阿尔缇玛基因组学公司 用于序列判定的方法和系统
AU2018353924A1 (en) 2017-12-29 2019-07-18 Clear Labs, Inc. Automated priming and library loading device
US20210079461A1 (en) * 2018-04-20 2021-03-18 The Regents Of The University Of California Nucleic Acid Sequencing Methods and Computer-Readable Media for Practicing Same
WO2020185790A1 (en) 2019-03-10 2020-09-17 Ultima Genomics, Inc. Methods and systems for sequence calling
US20220411863A1 (en) * 2019-11-25 2022-12-29 William Marsh Rice University Linear dna assembly for nanopore sequencing
WO2022008641A1 (en) 2020-07-08 2022-01-13 Roche Sequencing Solutions, Inc. Split-pool synthesis apparatus and methods of performing split-pool synthesis
CN116710571A (zh) 2020-10-15 2023-09-05 卡帕生物系统公司 用于下一代测序文库制备的电泳装置和方法
WO2022194764A1 (en) 2021-03-15 2022-09-22 F. Hoffmann-La Roche Ag Targeted next-generation sequencing via anchored primer extension
EP4314330A1 (de) 2021-03-26 2024-02-07 F. Hoffmann-La Roche AG Hybridisierungspufferformulierungen
EP4314337A1 (de) 2021-04-01 2024-02-07 F. Hoffmann-La Roche AG Immunzellenzählung von sars-cov-2-patienten auf der basis von immunrepertoiresequenzierung
IL307398A (en) * 2021-04-12 2023-12-01 Univ Hong Kong Chinese Base change analysis using electrical signals
WO2023023561A1 (en) * 2021-08-17 2023-02-23 Quantapore, Inc. Multiplex binding assays with optical nanopore readout
WO2023067597A1 (en) * 2021-10-18 2023-04-27 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Use of nanopore sequencing for determining the origin of circulating dna
WO2024003332A1 (en) 2022-06-30 2024-01-04 F. Hoffmann-La Roche Ag Controlling for tagmentation sequencing library insert size using archaeal histone-like proteins
CN114898802B (zh) * 2022-07-14 2022-09-30 臻和(北京)生物科技有限公司 基于血浆游离dna甲基化测序数据的末端序列频率分布特征确定方法、评价方法及装置
WO2024046992A1 (en) 2022-09-02 2024-03-07 F. Hoffmann-La Roche Ag Improvements to next-generation target enrichment performance

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Publication number Publication date
IL285795B (en) 2022-07-01
EP3334843A1 (de) 2018-06-20
TW202130815A (zh) 2021-08-16
IL294192A (en) 2022-08-01
JP2018531583A (ja) 2018-11-01
JP2021118691A (ja) 2021-08-12
HK1245850A1 (zh) 2018-08-31
US20220205038A1 (en) 2022-06-30
AU2016305103C1 (en) 2020-06-04
US20170044606A1 (en) 2017-02-16
SA518390907B1 (ar) 2021-09-08
US11319586B2 (en) 2022-05-03
IL257074A (en) 2018-03-29
HK1248286A1 (zh) 2018-10-12
AU2023202572A1 (en) 2023-05-18
AU2020202153A1 (en) 2020-04-16
AU2016305103A1 (en) 2018-03-22
IL257074B (en) 2021-09-30
EP3334843A4 (de) 2019-01-02
TWI728994B (zh) 2021-06-01
IL294192B1 (en) 2023-04-01
CN107849607A (zh) 2018-03-27
WO2017025059A1 (en) 2017-02-16
TW201718874A (zh) 2017-06-01
AU2020202153B2 (en) 2023-02-02
IL285795A (en) 2021-09-30
CN107849607B (zh) 2022-10-11
JP6867045B2 (ja) 2021-04-28
CA2995422A1 (en) 2017-02-16
IL294192B2 (en) 2023-08-01
MY188348A (en) 2021-12-02
JP7506408B2 (ja) 2024-06-26
TWI793586B (zh) 2023-02-21
AU2016305103B2 (en) 2020-01-02

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