CN115426874A - 烟草植物体及其制备方法 - Google Patents

烟草植物体及其制备方法 Download PDF

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Publication number
CN115426874A
CN115426874A CN202180029970.2A CN202180029970A CN115426874A CN 115426874 A CN115426874 A CN 115426874A CN 202180029970 A CN202180029970 A CN 202180029970A CN 115426874 A CN115426874 A CN 115426874A
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Prior art keywords
nicotiana
ccd4
polypeptide
tobacco
endogenous gene
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Pending
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CN202180029970.2A
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English (en)
Chinese (zh)
Inventor
真笼洋
新井雅雄
大山清
高仓由光
西口辽
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Japan Tobacco Inc
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Japan Tobacco Inc
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Publication of CN115426874A publication Critical patent/CN115426874A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/11—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/10—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits
    • A01H1/101—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine or caffeine
    • A01H1/107—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine or caffeine involving pigment biosynthesis
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
    • A01H6/823—Nicotiana, e.g. tobacco
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B13/00—Tobacco for pipes, for cigars, e.g. cigar inserts, or for cigarettes; Chewing tobacco; Snuff
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Biophysics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Physiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nutrition Science (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
CN202180029970.2A 2020-04-21 2021-04-21 烟草植物体及其制备方法 Pending CN115426874A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020-075565 2020-04-21
JP2020075565 2020-04-21
PCT/JP2021/016145 WO2021215465A1 (ja) 2020-04-21 2021-04-21 タバコ植物体とその製造方法

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Publication Number Publication Date
CN115426874A true CN115426874A (zh) 2022-12-02

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US (1) US20230071752A1 (https=)
EP (1) EP4140293A1 (https=)
JP (1) JPWO2021215465A1 (https=)
CN (1) CN115426874A (https=)
BR (1) BR112022021375A2 (https=)
WO (1) WO2021215465A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112022021375A2 (pt) * 2020-04-21 2022-12-06 Japan Tobacco Inc Corpo da planta de tabaco e método para produzir o mesmo
CN118475234A (zh) * 2021-12-27 2024-08-09 日本烟草产业株式会社 烟草植物
JPWO2023162949A1 (https=) * 2022-02-22 2023-08-31
CN116183779B (zh) * 2023-04-18 2025-01-24 云南省烟草农业科学研究院 基于一步净化和液相色谱-串联质谱的烟气中TSNAs测定方法

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007028115A2 (en) * 2005-09-02 2007-03-08 Cornell University Polynucleotides encoding carotenoid and apocarotenoid biosynthetic pathway
CN103205405A (zh) * 2013-04-11 2013-07-17 中国农业大学 蛋白IbGGPS及编码基因与在调控植物类胡萝卜素含量中的应用
CN104152475A (zh) * 2014-08-18 2014-11-19 中国烟草总公司郑州烟草研究院 烟草ε-番茄红素环化酶基因及其应用
CN104152473A (zh) * 2014-08-18 2014-11-19 中国烟草总公司郑州烟草研究院 烟草类胡萝卜素异构酶基因及其应用
JP2015505666A (ja) * 2011-10-31 2015-02-26 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物におけるβ−ダマセノンの調節
CN105154411A (zh) * 2015-07-31 2015-12-16 郑州轻工业学院 微生物双加氧酶粗酶液及β-紫罗兰酮的制备方法
CN105368850A (zh) * 2015-11-25 2016-03-02 天津大学 产生β-紫罗酮香气物质的枸杞类胡萝卜素裂解双加氧酶基因及应用
CN107502614A (zh) * 2017-03-22 2017-12-22 中国医学科学院药用植物研究所 一种参与栀子西红花苷合成的类胡萝卜素裂解双加氧酶编码基因的筛选及功能验证
CN108882689A (zh) * 2016-03-30 2018-11-23 日本烟草产业株式会社 烟草植物体及其制备方法
CN110546268A (zh) * 2017-02-24 2019-12-06 新加坡科技研究局 类胡萝卜素和脱辅基类胡萝卜素的生产
WO2021215465A1 (ja) * 2020-04-21 2021-10-28 日本たばこ産業株式会社 タバコ植物体とその製造方法
CN115485386A (zh) * 2020-04-17 2022-12-16 日本烟草产业株式会社 低生物碱含量的烟草属植物体及其制备方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102158632B1 (ko) * 2018-11-16 2020-09-23 경희대학교 산학협력단 카로티노이드 함량이 증진된 기능성 식물체 및 이의 제조 방법

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007028115A2 (en) * 2005-09-02 2007-03-08 Cornell University Polynucleotides encoding carotenoid and apocarotenoid biosynthetic pathway
US20090178156A1 (en) * 2005-09-02 2009-07-09 Nestec S.A. Polynucleotides encoding carotenoid and apocartenoid biosynthetic pathway enzymes in coffee
JP2015505666A (ja) * 2011-10-31 2015-02-26 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物におけるβ−ダマセノンの調節
CN103205405A (zh) * 2013-04-11 2013-07-17 中国农业大学 蛋白IbGGPS及编码基因与在调控植物类胡萝卜素含量中的应用
CN104152475A (zh) * 2014-08-18 2014-11-19 中国烟草总公司郑州烟草研究院 烟草ε-番茄红素环化酶基因及其应用
CN104152473A (zh) * 2014-08-18 2014-11-19 中国烟草总公司郑州烟草研究院 烟草类胡萝卜素异构酶基因及其应用
CN105154411A (zh) * 2015-07-31 2015-12-16 郑州轻工业学院 微生物双加氧酶粗酶液及β-紫罗兰酮的制备方法
CN105368850A (zh) * 2015-11-25 2016-03-02 天津大学 产生β-紫罗酮香气物质的枸杞类胡萝卜素裂解双加氧酶基因及应用
CN108882689A (zh) * 2016-03-30 2018-11-23 日本烟草产业株式会社 烟草植物体及其制备方法
CN110546268A (zh) * 2017-02-24 2019-12-06 新加坡科技研究局 类胡萝卜素和脱辅基类胡萝卜素的生产
CN107502614A (zh) * 2017-03-22 2017-12-22 中国医学科学院药用植物研究所 一种参与栀子西红花苷合成的类胡萝卜素裂解双加氧酶编码基因的筛选及功能验证
CN115485386A (zh) * 2020-04-17 2022-12-16 日本烟草产业株式会社 低生物碱含量的烟草属植物体及其制备方法
WO2021215465A1 (ja) * 2020-04-21 2021-10-28 日本たばこ産業株式会社 タバコ植物体とその製造方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GAO,J.P.等: ""Nicotiana tabacum cultivar Samsun carotenoid cleavage dioxygenase 4 (CCD4) mRNA,complete cds"", pages 1 *
KENTA WATANABE等: ""Alteration of flower colour in Ipomoea nil through CRISPR/Cas9-mediated mutagenesis of carotenoid cleavage dioxygenase 4"", vol. 27, no. 1, pages 1 - 14 *
QIANQIAN ZHOU等: ""Carotenoid Cleavage Dioxygenases: Identification, Expression, and Evolutionary Analysis of This Gene Family in Tobacco"", vol. 20, pages 1 - 24 *

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BR112022021375A2 (pt) 2022-12-06
JPWO2021215465A1 (https=) 2021-10-28
US20230071752A1 (en) 2023-03-09
WO2021215465A1 (ja) 2021-10-28
EP4140293A1 (en) 2023-03-01

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