CN115413667A - Preparation for relieving corn cadmium stress and application thereof - Google Patents
Preparation for relieving corn cadmium stress and application thereof Download PDFInfo
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- CN115413667A CN115413667A CN202211124266.8A CN202211124266A CN115413667A CN 115413667 A CN115413667 A CN 115413667A CN 202211124266 A CN202211124266 A CN 202211124266A CN 115413667 A CN115413667 A CN 115413667A
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- cadmium
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- melatonin
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- 229910052793 cadmium Inorganic materials 0.000 title claims abstract description 80
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 title claims abstract description 79
- 240000008042 Zea mays Species 0.000 title claims abstract description 45
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 45
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 40
- 235000005822 corn Nutrition 0.000 title claims abstract description 40
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- YJPIGAIKUZMOQA-UHFFFAOYSA-N Melatonin Natural products COC1=CC=C2N(C(C)=O)C=C(CCN)C2=C1 YJPIGAIKUZMOQA-UHFFFAOYSA-N 0.000 claims abstract description 40
- DRLFMBDRBRZALE-UHFFFAOYSA-N melatonin Chemical compound COC1=CC=C2NC=C(CCNC(C)=O)C2=C1 DRLFMBDRBRZALE-UHFFFAOYSA-N 0.000 claims abstract description 40
- 229960003987 melatonin Drugs 0.000 claims abstract description 39
- 229910052710 silicon Inorganic materials 0.000 claims abstract description 27
- 239000010703 silicon Substances 0.000 claims abstract description 27
- 230000012010 growth Effects 0.000 claims abstract description 22
- 241001057636 Dracaena deremensis Species 0.000 claims abstract description 14
- 238000009825 accumulation Methods 0.000 claims abstract description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 20
- 239000003795 chemical substances by application Substances 0.000 claims description 18
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- 239000004115 Sodium Silicate Substances 0.000 claims description 9
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 claims description 9
- 229910052911 sodium silicate Inorganic materials 0.000 claims description 9
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- 238000011160 research Methods 0.000 description 2
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- IXVMHGVQKLDRKH-QHBHMFGVSA-N 24-Epibrassinolide Natural products C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@@H](O)[C@H](O)[C@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-QHBHMFGVSA-N 0.000 description 1
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- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 description 1
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- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- IXVMHGVQKLDRKH-KNBKMWSGSA-N brassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@@H](O)[C@H](O)[C@@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-KNBKMWSGSA-N 0.000 description 1
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical class [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 1
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- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
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Images
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Soil Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a preparation for relieving corn cadmium stress and application thereof. Belongs to the technical field of crop physiology. The invention adopts the compounding use of the melatonin and the silicon, and proves that the melatonin can promote the growth of corn plants under cadmium stress, promote the accumulation of corn plant dry matters under cadmium stress, promote the growth of the height and roots of the corn plants under cadmium stress and reduce the cadmium content of corn leaves and roots under cadmium stress. Provides a new technical approach for applying melatonin and silicon in field corn safety production and increasing yield. Has important significance for realizing the safe, efficient and green sustainable development of agriculture.
Description
Technical Field
The invention relates to the technical field of crop physiology, in particular to a preparation for relieving corn cadmium stress and application thereof.
Background
Corn is the first large grain crop in China. In recent years, due to mineral exploitation, industrial wastewater and waste gas emission, and the use of a large amount of chemical fertilizers and pesticides, farmland soil is polluted by heavy metal cadmium, and the phenomenon that the cadmium in crops exceeds the standard frequently occurs all over the country.
In recent years, numerous methods and approaches to reduce cadmium uptake and accumulation in plants have been reported. The absorption and the transportation of cadmium in plants can be effectively reduced by using an agricultural regulation and chemical method, and researches show that biochar, exogenous zinc, selenium, silicon and the like can improve the growth of plants and relieve the influence of the plants under the cadmium stress. The use of exogenous regulating substances can also effectively reduce the absorption and accumulation of cadmium by plants and reduce the transfer of cadmium from root systems to overground parts. Such as abscisic acid, 24-epibrassinolide, ethylene, auxin, salicylic acid, melatonin and the like can obviously promote the growth of plants.
However, in the prior art, a single using method is more and the regulation and control effect is limited.
In conclusion, the problem to be solved by the technical personnel in the field is how to provide a preparation for relieving the cadmium stress of corn and application thereof.
Disclosure of Invention
In view of this, the invention provides a preparation for relieving corn cadmium stress and an application thereof. The invention has a superposition effect by compositely applying the melatonin and the silicon, and provides an eco-friendly feasible method for relieving the cadmium toxicity.
In order to achieve the purpose, the invention adopts the following technical scheme:
a preparation for relieving cadmium stress of corn comprises an agent A and an agent B;
the A agent comprises melatonin, an adhesive, an ethanol solution and water, wherein the mass volume ratio of the melatonin to the adhesive to the ethanol solution is 1.16g, (0.5-1) ml:1000ml
The agent B comprises sodium silicate.
The beneficial effects are as follows: melatonin, also known as N-acetyl-5-methoxytryptamine, is an indoleamine derived from tryptophan and has pleiotropic regulatory effects in plants. Silicon is a chemical element, a metalloid element of IVA group, widely exists in rock, gravel and dust in the form of silicate or silicon dioxide, is the second most abundant element in the earth crust, is second only to oxygen, and is a beneficial element for plant growth and development. Proper amount of silicon can promote the growth and development of plants, enhance the photosynthetic capacity of the plants, improve the photosynthetic efficiency and the accumulation of crop biomass, promote the absorption of corn to nutrients, improve the corn yield, simultaneously improve the resistance of the plants to heavy metal stress and reduce the absorption and accumulation of the corn to cadmium.
The melatonin and the silicon can promote the growth and development of plants, promote the growth of lateral roots, regulate the photoperiod and regulate the yield, increase the yield, delay aging and enhance the stress tolerance of the plants, can effectively relieve the toxic action of cadmium stress on the plants and reduce the absorption and the transportation of the plants to cadmium. The invention researches and analyzes the influence of the compound regulation of the melatonin and the silicon on the tolerance of the corn under the cadmium stress, and provides a new technical approach for the compound application of the melatonin and the silicon to the safe production and the yield increase of the field corn. Has important significance for realizing the safe, efficient and green sustainable development of agriculture.
The melatonin and the silicon are compounded and applied to have a synergistic effect, and both the melatonin and the silicon can improve the oxidative stress caused by cadmium stress and improve the resistance of crops to play a positive role in growth; the silicon can isolate heavy metals to an area with inactive metabolism and chelate the heavy metals to form coprecipitation, and the melatonin can promote cell walls or vacuoles to chelate cadmium to limit the transport of the cadmium from roots to stems; in addition, both can inhibit the expression of cadmium related genes and minimize the absorption and toxicity of cadmium in roots. The two are combined to provide an eco-friendly feasible method for relieving the cadmium toxicity.
Further, the adhesive comprises polyvinyl alcohol and cellulose, and the mass ratio of the polyvinyl alcohol to the cellulose is 2.8.
The beneficial effects are as follows: the mass ratio can be uniformly used for coating seeds, and the coating has the characteristic of better film forming property.
Further, the ethanol solution is absolute ethanol.
The beneficial effects are as follows: the anhydrous ethanol solution can rapidly dissolve melatonin.
The preparation is applied to relieving corn cadmium stress.
Further, the preparation relieves corn cadmium stress through at least one of the following (1) to (4):
(1) Promoting the growth of corn plants under cadmium stress;
(2) Promoting the growth of the plant height and the roots of the corn under the cadmium stress;
(3) Promoting the accumulation of dry matters of corn plants under the cadmium stress;
(4) The cadmium content of the corn leaves and roots under the cadmium stress is reduced.
Further, the method comprises the following specific steps:
(1) Preparation of the agent A: dissolving melatonin in an ethanol solution, adding water, adding an adhesive, and uniformly stirring;
(2) Preparation of agent B: dissolving sodium silicate in water, wherein the mass volume ratio of the sodium silicate to the water is (0.318g);
(3) Dressing corn seeds with the agent A:
spraying the preparation A onto semen Maydis, stirring with a stirrer to coat a layer of medicinal film on the surface of the semen Maydis, and air drying.
The seed dressing is generally carried out one day before sowing, and the safety of the medicine is required during seed dressing. If the seeds are manually mixed, the uniform application of the medicine needs to be noticed. In the case of mechanical seed dressing, it is necessary to prevent the seeds from rolling excessively in the machine and from damaging the seeds. The seeds after being mixed are required to be slowly dried in the shade in a room, and the seeds are prevented from being irradiated by sunlight or dried by strong wind;
(4) The silicon is provided by sodium silicate: na (Na) 2 SiO 3 ·5H 2 The using amount of O is 1.5mmol/L;
(5) And (6) sowing.
Furthermore, the dosage of the agent A is 2-3% of the mass of the corn seeds.
The beneficial effects are as follows: the seeds were coated uniformly and consistently.
According to the technical scheme, compared with the prior art, the invention has the following beneficial effects: the invention adopts the compounding use of the melatonin and the silicon, and proves that the melatonin can promote the growth of corn plants under the cadmium stress, promote the accumulation of dry matters of the corn plants under the cadmium stress, promote the growth of the height and roots of the corn plants under the cadmium stress and reduce the cadmium content of leaves and roots of the corn plants under the cadmium stress. Provides a new technical approach for applying melatonin and silicon in field corn safety production and increasing yield. Has important significance for realizing the safe, efficient and green sustainable development of agriculture.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a diagram showing the growth of a maize plant under cadmium stress conditions in experiment 1 of the present invention, wherein a is the growth of the aerial parts and b is the growth of the root system;
FIG. 2 is a diagram showing the plant height of a maize plant under cadmium stress conditions in experiment 1 of the present invention;
FIG. 3 is a graph showing the growth of the main root under cadmium stress conditions in experiment 1;
FIG. 4 is a graph showing the dry weight of plants under cadmium stress conditions in experiment 1;
FIG. 5 is a graph showing the cadmium content of different parts of corn under cadmium stress in experiment 1.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
The required medicament in the embodiment of the invention is a conventional experimental medicament purchased from a commercial channel; the experimental methods not mentioned in the examples are conventional experimental methods, and are not described in detail herein.
Example 1
(1) Dissolving 1.16g of melatonin in 1ml of absolute ethanol solution, and then adding 1L of water to obtain solution A;
(2) Dissolving 2.8g of polyvinyl alcohol and 3.2g of cellulose in the solution A, and uniformly mixing and stirring to obtain a suspending agent, namely the agent A;
(3) Seed dressing is carried out 1 day before sowing, and the corn seeds are dressed by using the agent A;
(4) The silicon is provided by sodium silicate and 0.318g Na is weighed 2 SiO 3 ·5H 2 Dissolving O in 1L of water;
(5) And (5) sowing.
(1) The experimental site: the test was carried out in the climatic chamber of the science and technology institute of Henan, 2021, 6 months. Experimental treatment: selecting corn seeds with uniform shape and size by water culture method, and adding 10% of the corn seeds 2 O 2 Sterilizing for 5min, washing with tap water for 6-8 times, and mixing with Cd, MT50+ Cd, si + Cd, MT50+ Si + Cd. The treatment containing silicon and cadmium dissolves silicon and cadmium in the sterilized saturated calcium sulfate solution, and the dosage is respectively as follows: 1.5mmol/L (Na) 2 SiO 3 ·5H 2 O)、Cd：35mg/L(CdCl 2 ) (ii) a The treatment containing melatonin adopts a melatonin solution and a seed coating agent for seed dressing (the seed coating agent is 0.32g/100ml of cellulose and 0.28g/100ml of polyvinyl alcohol), and the treatment without melatonin adopts clear water and the seed coating agent for seed dressing (the seed coating agent is the same as above);
group Cd: subjected to cadmium stress and without the use of the preparation prepared by the invention;
si + Cd group: cadmium-stressed and silicon-only treatment;
MT50+ Cd group: cadmium stressed and melatonin treatment only;
MT50+ Si + Cd group: cadmium stressed and treated according to the method of example 1 of the present invention.
Measurement indexes are as follows:
after culturing for 18 days, selecting corn seedlings with consistent growth vigor, repeating each treatment for 4 times, and measuring morphological indexes of the corn subjected to different treatments, such as plant height, root system characteristics, leaf projection area, dry weight, cadmium content of different parts and the like.
(1) The determination method comprises the following steps:
plant height, main root length: the length of the main root and the plant height were measured manually with a ruler. Each treatment was 4 replicates.
Root form characteristics and leaf scanning: and respectively taking 4 seedlings with different treatments and consistent growth vigor, and repeating. And scanning the shape and the blades of the whole root system by an EPSON scanner, and analyzing the total root length, the total root surface area, the total root volume, the average root diameter and the blade projection area by using Win RHIO software.
Dry weight: separating the root system and overground part of the selected plant, putting the fresh sample into a 105 ℃ oven for 30min for enzyme deactivation, drying at 80 ℃ to constant weight, respectively weighing the dry weight of the overground part and the dry weight of the root system, and repeating each treatment for 3 times.
And (3) determination of cadmium content: grinding and crushing each part of the weighed plant, weighing 0.05g of a sample in a tetrafluoro crucible, adding 20ml of mixed solution (nitric acid: perchloric acid =3: 1) for nitrolysis for 12h, putting the tetrafluoro crucible on an electric heating plate (about 170 ℃) in a fume hood for acid removal, stopping acid removal when the volume of the solution is changed into the size of soybean, cooling, adding 3% nitric acid to 25ml, and shaking uniformly for later use. And finally, measuring the cadmium content by using an Optima2100DV inductively coupled plasma emission spectrometer of the American PE company.
(2) As a result: the results are shown in FIGS. 1 to 5 and Table 1.
As can be seen from fig. 1 to 5 and table 1: the Cd group obviously inhibits the growth of the overground part and the root system of the corn plant, reduces the plant height and the root system length, and simultaneously reduces the dry matter accumulation of the plant; the MT50+ Si + Cd group remarkably promotes the growth of the overground part and the root system of the corn plant under the cadmium stress condition, increases the plant height and the root system length, increases the dry matter accumulation amount of the plant, and reduces the accumulation of cadmium in the root system and leaves.
As can be seen from FIGS. 1 and 2, the growth of the overground part and the root system of the maize seedlings is inhibited under the cadmium stress, the plant height treated by MT50+ Cd is slightly reduced, but the plant height treated by Si + Cd and MT50+ Si + Cd is obviously higher than that treated by Cd.
As can be seen from figure 3, cadmium stress inhibits the length of the main root of the corn seedling, the promotion effect of the external regulating substance on the main root of the corn under the cadmium stress is different, the maximum significant effect of the silicon and melatonin on the main root length of the corn in composite application is greater than that of other treatments, the application of the silicon also has a significant promotion effect on the main root growth of the corn, and the influence of the melatonin dressing treatment on the main root length is not significantly different from that of the Cd treatment.
TABLE 1 melatonin dressing and its effect on corn root characteristics and leaf area under cadmium stress conditions with silicon treatment
Under the condition of cadmium stress, the application of melatonin and silicon promotes the growth and development of the corn root system. Under the condition of cadmium stress, the total root length, the root volume and the root surface area of the corn are all shown to be superior to the single application of silicon and melatonin under the compound treatment of the melatonin and the silicon, the average diameter of the roots has no obvious difference, the projection area of the leaf blade has the minimum melatonin treatment, and other treatments have no obvious difference.
As can be seen from FIG. 4, cadmium stress reduced the dry matter accumulation in the aerial parts of the maize plants, and there was no significant change in the underground root system. Under the condition of cadmium stress, the melatonin dressing can also increase the dry weight of the corn leaves and the root systems, the effect of the composite treatment of the melatonin dressing and the root systems is also better than that of the single treatment, but the difference between the treatments does not reach a remarkable level.
As can be seen from fig. 5, different melatonin treatment methods and their application with silicon can significantly reduce the accumulation of melatonin in various parts of plants. The cadmium content in the root system is obviously higher than that in the leaves. Under the condition of cadmium stress, compared with Cd treatment, the cadmium content in the leaves of the seedlings treated by Si + Cd, MT50+ Cd and MT50+ Si + Cd in the melatonin dressing treatment is respectively reduced by 72.90%, 24.89% and 76.37%; the root systems are respectively 9.42 percent, 1.84 percent and 13.76 percent.
In the present specification, the embodiments are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (7)
1. A preparation for relieving corn cadmium stress is characterized by comprising an agent A and an agent B;
the A agent comprises melatonin, an adhesive, an ethanol solution and water, wherein the mass volume ratio of the melatonin to the adhesive to the ethanol solution is 1.16g, (0.5-1) ml:1000ml;
the agent B comprises sodium silicate.
2. The corn cadmium stress relieving preparation of claim 1, wherein the adhesive comprises polyvinyl alcohol and cellulose in a mass ratio of 2.8.
3. The corn cadmium stress relieving preparation of claim 1, wherein the ethanol solution is absolute ethanol.
4. Use of a formulation according to any one of claims 1 to 3 for the alleviation of cadmium stress in maize.
5. The use of claim 4, wherein the formulation relieves corn cadmium stress by at least one of (1) to (4):
(1) Promoting the growth of corn plants under cadmium stress;
(2) Promoting the accumulation of dry matter of corn plants under cadmium stress;
(3) Promoting the growth of the height and the roots of the corn plants under the cadmium stress;
(4) The cadmium content of the corn leaves and roots under the cadmium stress is reduced.
6. The application as claimed in claim 4, characterized by the specific steps of:
(1) Preparation of the agent A: dissolving melatonin in an ethanol solution, adding water, adding an adhesive, and uniformly stirring;
(2) Preparation of the agent B: dissolving sodium silicate in water, wherein the mass volume ratio of the sodium silicate to the water is 0.318g;
(3) Dressing corn seeds with the agent A;
(4) The silicon is provided by sodium silicate: na (Na) 2 SiO 3 ·5H 2 The dosage of O is 1.5mmol/L;
(5) And (6) sowing.
7. The use according to claim 6, wherein the agent A is used in an amount of 2 to 3% by mass of the corn seeds.
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| 李清芳;马成仓;尚启亮;: "干旱胁迫下硅对玉米光合作用和保护酶的影响", 应用生态学报, no. 03 * |
| 王丙烁 等: "镉胁迫下不同改良剂对水稻种子萌发和镉吸收积累的影响", 《农业环境科学学报》, vol. 38, no. 4, pages 748 - 753 * |
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