CN115405133A - Environmental barrier method suitable for HIV laboratory - Google Patents
Environmental barrier method suitable for HIV laboratory Download PDFInfo
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- CN115405133A CN115405133A CN202211149886.7A CN202211149886A CN115405133A CN 115405133 A CN115405133 A CN 115405133A CN 202211149886 A CN202211149886 A CN 202211149886A CN 115405133 A CN115405133 A CN 115405133A
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Abstract
The invention provides an environmental barrier method suitable for an HIV laboratory, which is characterized by comprising the following steps: comprises an HIV experiment system body; the HIV experiment system body comprises a buffer part, a sterilization part, a serum part, an HIV detection part, a transportation part and a sample channel; wherein the buffer part, the sterilization part, the serum part, the HIV detection part, the transportation part and the sample channel are all provided with pressure regulation and control equipment; the pressure regulating and controlling equipment independently regulates and controls the pressure of the corresponding spaces of the buffer part, the sterilizing part, the serum part, the HIV detecting part, the transporting part and the sample channel; the system effectively blocks dust particles and microorganisms outside the laboratory from entering the laboratory, effectively ensures the safety of the working environment, and effectively ensures the cleanliness of a core HIV detection room inside the laboratory.
Description
Technical Field
The invention provides medical aids, and in particular relates to an environmental barrier method and system suitable for use in an HIV laboratory.
Background
HIV laboratory tests human blood, tissue fluid, excrement, tissue, organ, semen and related blood products, biological tissues or other articles for AIDS virus (HIV) or its corresponding markers, including HIV separation, HIV antigen detection, nucleic acid detection and HIV antibody detection. In view of the fact that the conventional detection method is used for detecting HIV antibodies in blood at present, the national AIDS detection technical specification emphasizes that corresponding regulations are made on the detection work.
According to the regulations on biological safety management in pathogenic microorganism laboratories, human Immunodeficiency Virus (HIV) belongs to highly pathogenic microorganisms and is classified as a pathogenic microorganism of the 2 nd category of hazard degree.
The serological, immunological and nucleic acid tests for AIDS should be carried out in AIDS testing laboratories meeting the requirements of the class II biosafety laboratory (BSL-2). The laboratory is required to meet all the requirements of the building technical specifications of a laboratory biological safety general requirement (GB 19489-2008) level II biological safety laboratory and a laboratory biological safety laboratory (GB 50346-2011), and the cleanliness is designed according to ISO level 8 (hundred thousand level).
At present, various schemes exist for HIV laboratory environment barrier process technology, but the common defects are that scientific and reasonable personnel flow, article flow and pollutant flow do not exist, the article flow and the pollutant flow are not separated, reasonable and necessary room composition does not exist, laboratory airflow pressure gradient is not reasonably divided, a positive pressure air lock is not adopted to separate a biological safety area from the external environment of a laboratory, and reliable airflow blocking cannot be realized.
Generally, the defects easily cause the overflowing of dangerous biological factors to pollute the external environment of a laboratory, harm is caused to human health, the internal cleanliness can not be guaranteed easily, and cross infection influences the experimental result.
Disclosure of Invention
The invention aims to overcome the defects and provide an environmental barrier method suitable for an HIV laboratory through structural modification and reasonable planning and layout for the purposes of improving the biological safety stability and reliability of the operation of the HIV laboratory and the accuracy and reliability of virus detection.
The invention provides an environmental barrier method suitable for an HIV laboratory, which is characterized by comprising the following steps:
setting up an HIV experiment system based on a workflow, a logistics and a pollutant logistics;
the HIV experiment system comprises an HIV experiment system body;
the HIV experiment system body comprises a buffer part, a sterilization part, a serum part, an HIV detection part, a transportation part and a sample channel;
wherein, the buffer part, the sterilization part, the serum part, the HIV detection part, the transportation part and the sample channel are all provided with pressure regulation and control equipment;
the pressure regulating and controlling equipment independently regulates and controls the pressure of the corresponding spaces of the buffer part, the sterilizing part, the serum part, the HIV detecting part, the transporting part and the sample channel;
the buffer part, the sterilization part, the serum part and the HIV detection part are independent from each other and are respectively communicated with the transportation part through different ports;
the sample channel is arranged between the buffer part and the sterilization part;
one end of the sample channel is in butt joint with the transportation part, and the other end of the sample channel is provided with a sample input window;
the serum part is communicated with the HIV detection part through a sample transfer window.
Further, the present invention provides an environmental barrier method suitable for HIV laboratories, which is further characterized by:
the pressure of the buffer is set to be the highest.
Further, the present invention provides an environmental barrier method suitable for HIV laboratory, which is characterized by:
the pressure of the HIV detection unit was set to the lowest.
Further, the present invention provides an environmental barrier method suitable for HIV laboratory, which is characterized by:
the sterilizing part is positive pressure relative to the transporting part.
Further, the present invention provides an environmental barrier method suitable for HIV laboratories, which is further characterized by:
the data processing unit, the sterilization unit, and the buffer unit are all at positive pressure.
Further, the present invention provides an environmental barrier method suitable for HIV laboratories, which is further characterized by:
the serum part, the transportation part, the sample channel and the HIV detection part are all negative pressure.
Further, the present invention provides an environmental barrier method suitable for HIV laboratory, which is characterized by:
the pressure of the serum part is less than that of the transportation part.
Further, the present invention provides an environmental barrier method suitable for HIV laboratory, which is characterized by:
one side of the buffer part is separated from the transportation part by a partition, and a channel communicated with the transportation part is arranged on the buffer part;
the other side of the buffer part is separated from the sample channel by a partition;
the sterilizing part is arranged in parallel with the buffer part;
the first side of the sterilization part is separated from the sample channel by a partition;
the second side of the sterilizing part is separated from the transporting part by a partition, and a passage communicated with the transporting part is arranged on the second side of the sterilizing part;
the third side of the sterilization part is separated from the serum part by a partition;
the fourth side of the sterilizing part is separated from the external environment by a partition and is provided with a pollutant discharge channel;
the HIV detection part is arranged opposite to the buffer part;
the HIV detection part is separated from the transportation part through a partition, and a passage communicated with the transportation part is arranged on the HIV detection part.
Further, the present invention provides an environmental barrier method suitable for HIV laboratories, which is further characterized by:
also includes a data processing part;
the data processing part and the buffer part are provided with mutually communicated channels.
Further, the present invention provides an environmental barrier method suitable for HIV laboratories, which is further characterized by:
also includes an electromechanical part;
the electromechanical part controls the electrical devices of each part.
Further, the present invention provides an environmental barrier method suitable for HIV laboratories, which is further characterized by:
each part has a visible medium. Such as: transparent material partition, or visual screen/monitoring device, etc.
The invention has the following functions and effects:
the invention has reasonable arrangement of each functional area, and after the circulation channel is put into application, no matter the circulation channel is operated by personnel on the spot or by an automatic robot, the smooth operation of the working flow, the material flow and the polluted material flow can be simultaneously met, and the circulation channel has no virus leakage and environmental pollution and no cross infection in the detection process; the method meets the requirements of HIV virus detection process and HIV screening laboratory working process.
According to the invention, through gradient control of different pressures of each unit, harmful biological factors in the test area are effectively prevented from escaping from the biological safety area, dust particles and microorganisms outside the laboratory are effectively prevented from entering the laboratory, the safety of the working environment is effectively guaranteed, and the cleanliness of a core HIV detection room inside the laboratory is effectively guaranteed.
Drawings
FIG. 1 is a schematic diagram of an environmental barrier method suitable for HIV laboratories.
Detailed Description
This example provides an environmental barrier method suitable for use in the HIV laboratory.
In the present method, the HIV laboratory is divided into: a clean area (a data processing chamber, a buffer chamber and a sterilization chamber), a semi-polluted area (a corridor), a polluted area (an HIV virus detection part), a serum part and an electromechanical part;
(1) A cleaning area: data processing, changing clothes, washing hands and sterilizing;
(2) A semi-polluted area: internal people flow, logistics, sample pretreatment and the like;
(3) A pollution area: detecting HIV virus;
(4) Serum fraction: serum, kit short term and long term storage.
(5) An electromechanical part: the cleanliness, the temperature, the humidity, the fresh air volume and the pressure gradient among rooms of the laboratory are guaranteed.
As shown by arrows in FIG. 1, the environmental barrier method for HIV laboratory according to the present embodiment is to set up an HIV laboratory system based on workflow, logistics and pollutant;
the workflow refers to: data processing department-buffer department-semi-polluted region corridor (transportation department) -polluted region HIV virus detection department or serous department;
the logistics mean: a transmission window X, a serum sample and detection kit storage serum part, a transmission window G2 and a contamination region HIV virus detection part; (the pass-through window is the mechanical interlocking door with ultraviolet ray disinfection)
The sewage flow means: dirt-sterilization part-outdoor;
based on the three flows, the present embodiment relates to an HIV experiment system, which includes an HIV experiment system body 100, a data processing part 200, and an equipment room 300;
wherein, the HIV experiment system body comprises a buffer part 110, a sterilization part 120, a serum part 130, an HIV detection part 140, a transportation part 150 and a sample channel 160;
the buffer part 110, the sterilization part 120, the serum part 130, the HIV detection part 140, the transportation part 150 and the sample channel 160 are all provided with pressure regulation and control equipment;
the pressure regulating device of the device is regulated and controlled by a device room 300, and the pressure of the space corresponding to the buffer part 110, the sterilizing part 120, the serum part 130, the HIV detecting part 140, the transportation part 150 and the sample channel 160 is independent;
wherein, the pressure of the buffer part is set to be the highest, the pressure of the HIV detection part is set to be the lowest, and the sterilization part is positive pressure relative to the transportation part.
Preferably, in one aspect of the present embodiment, the pressure between each part is set as follows:
in the preferred scheme of this embodiment, buffer portion pressure is the highest, and HIV virus detection portion pressure is the lowest, and when sterilization portion relative transport portion and the external environment of casing malleation, when keeping the pressure value of these three key nodes for above-mentioned law, effectively prevented that the inside harmful biological factor of experimental area from escaping the biological safety district, effectively blocked inside experimental system outside dust particle and the microorganism got into experimental system, effectively ensured environmental safety, effectively ensured the inside core HIV detection room cleanliness factor of experimental system.
The buffer part 110, the sterilization part 120, the serum part 130 and the HIV detection part 140 are independent from each other and are respectively communicated with the transportation part 150 through different ports;
the first side of the buffer part 110 is separated from the transportation part 140 by a partition A1, and a passage A1 communicated with the transportation part is arranged on the partition;
the second side of the buffer portion 110 is separated from the sample channel 160 by a partition B;
the third side of the buffer part 110 is separated from the data processing part 200 by a partition C, and is provided with a channel C1 communicated with the transportation part;
the fourth side of the buffer part 110 is a shell of the HIV experiment system body;
the sterilization part 120 is provided in parallel with the buffer part 110;
the first side of the sterilization part 120 is separated from the sample passage 160 by a partition D;
the second side of the sterilization part 120 is separated from the transportation part 150 by a partition E, and a passage E1 communicated with the transportation part 150 is arranged on the second side;
the third side of the sterilization part 120 is separated from the serum part 130 by a partition F;
the fourth side of the sterilizing part 120 is a casing of the HIV experiment system body, and a pollutant discharge channel X is arranged on the casing;
the HIV detection unit 140 disposed opposite to the buffer unit 110;
the HIV detection part 140 is separated from the transportation part 150 by a partition G, and a passage G1 communicated with the transportation part is arranged on the HIV detection part;
the extension segment of the partition G is the partition between the serum part 130 and the HIV detection part 140, and a sample transfer window G2 is arranged on the extension segment;
the sample channel 160 is disposed between the buffer portion 110 and the sterilization portion 120;
the sample channel 160, the end not butted with the buffer part 110 and the sterilization part 120, is butted with the transportation part, and the other end is a shell of the HIV experiment system body, and a sample input window Y is arranged on the shell;
in addition, one side of the HIV detection part 140 is separated from the data processing part by an extension segment of a partition C;
the other side of the HIV detection part 140 is separated from the equipment room 300 by a partition H;
the extension section of the partition H forms a partition between the serum part 130 and the equipment room 300;
the HIV detection part 140 is independently provided with a clean air conditioning unit, and the laboratory is completely ventilated with all fresh air.
The equipment room 300 is arranged on one side of the main body experiment system, is far away from the data processing part, and reduces the influence of noise on the working environment.
In addition, visual windows are arranged among all parts, so that the working environment is spacious and bright and more humanized, and the working pressure and the mental fatigue of experimenters in narrow spaces are relieved.
Action and effect of the present embodiment:
the embodiment firstly uses reasonable process technology subareas, necessary composition of the experiment units and airflow pressure gradient parameter setting to ensure smooth detection flow, reliable airflow blocking, prevention of external environment pollution caused by leakage of harmful biological factors in the HIV detection experiment system, guarantee of internal cleanliness, prevention of cross pollution among the experiment units in the HIV detection experiment system, and accurate and reliable detection.
Claims (10)
1. An environmental barrier method suitable for use in an HIV laboratory, comprising:
setting up an HIV experiment system based on a workflow, a logistics and a pollutant logistics;
the HIV experiment system comprises an HIV experiment system body;
the HIV experiment system body comprises a buffer part, a sterilization part, a serum part, an HIV detection part, a transportation part and a sample channel;
wherein the buffer part, the sterilization part, the serum part, the HIV detection part, the transportation part and the sample channel are all provided with pressure regulation and control equipment;
the pressure regulating and controlling equipment is used for independently regulating and controlling the pressure of the corresponding spaces of the buffer part, the sterilization part, the serum part, the HIV detection part, the transportation part and the sample channel;
the buffer part, the sterilization part, the serum part and the HIV detection part are independent from each other and are respectively communicated with the transportation part through different ports;
the sample channel is arranged between the buffer part and the sterilization part;
one end of the sample channel is in butt joint with the transportation part, and the other end of the sample channel is provided with a sample input window;
the serum part is communicated with the HIV detection part through a sample transfer window.
2. An environmental barrier method suitable for use in an HIV laboratory according to claim 1 wherein: the pressure of the buffer is set to be highest.
3. An environmental barrier method applicable to HIV laboratories, according to claim 1, characterized in that: the pressure of the HIV test part was set to be the lowest.
4. An environmental barrier method suitable for use in an HIV laboratory according to claim 1 wherein: the sterilization part is positive pressure relative to the transportation part.
5. An environmental barrier method suitable for use in an HIV laboratory according to claim 1 wherein: the data processing part, the sterilization part and the buffer part are all positive pressure.
6. An environmental barrier method suitable for use in an HIV laboratory according to claim 1 wherein: the serum part, the transportation part, the sample channel and the HIV detection part are all negative pressure.
7. An environmental barrier method applicable to HIV laboratories, according to claim 1, characterized in that: the serum part pressure is less than the transport part pressure.
8. An environmental barrier method applicable to HIV laboratories, according to claim 1, characterized in that: one side of the buffer part is separated from the transportation part through a partition, and a channel communicated with the transportation part is arranged on the buffer part; the other side of the buffer part is separated from the sample channel through a partition;
the sterilizing part is arranged in parallel with the buffer part;
the first side of the sterilizing part is separated from the sample channel by a partition;
the second side of the sterilizing part is separated from the transportation part through a partition, and a passage communicated with the transportation part is arranged on the second side of the sterilizing part; the third side of the sterilization part is separated from the serum part through a partition;
the fourth side of the sterilizing part is separated from the external environment by a partition, and a pollutant discharge channel is arranged on the fourth side of the sterilizing part;
the HIV detection part is arranged opposite to the buffer part;
the HIV detection part is separated from the transportation part by a partition, and a passage communicated with the transportation part is arranged on the HIV detection part.
9. An environmental barrier method, suitable for use in an HIV laboratory, according to any one of claims 1 to 8, wherein:
also includes a data processing part;
the data processing part and the buffer part are provided with mutually communicated channels.
10. An environmental barrier method, suitable for use in an HIV laboratory, according to any one of claims 1 to 8, wherein:
also includes an electromechanical part;
the electromechanical part controls the electrical devices of each part.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211149886.7A CN115405133A (en) | 2022-09-21 | 2022-09-21 | Environmental barrier method suitable for HIV laboratory |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211149886.7A CN115405133A (en) | 2022-09-21 | 2022-09-21 | Environmental barrier method suitable for HIV laboratory |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115405133A true CN115405133A (en) | 2022-11-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211149886.7A Pending CN115405133A (en) | 2022-09-21 | 2022-09-21 | Environmental barrier method suitable for HIV laboratory |
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| CN (1) | CN115405133A (en) |
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- 2022-09-21 CN CN202211149886.7A patent/CN115405133A/en active Pending
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