CN115399472A - Preparation method and application of probiotic enzyme powder with weight-losing function - Google Patents
Preparation method and application of probiotic enzyme powder with weight-losing function Download PDFInfo
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- 239000006041 probiotic Substances 0.000 title claims abstract description 50
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 50
- 239000000843 powder Substances 0.000 title claims abstract description 41
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 40
- 238000002360 preparation method Methods 0.000 title claims abstract description 36
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 34
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 34
- 240000002853 Nelumbo nucifera Species 0.000 claims abstract description 44
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims abstract description 44
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims abstract description 44
- 238000002386 leaching Methods 0.000 claims abstract description 44
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical class O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 claims abstract description 31
- 241001523681 Dendrobium Species 0.000 claims abstract description 10
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 235000005911 diet Nutrition 0.000 claims abstract description 6
- 230000037213 diet Effects 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims description 54
- 238000000855 fermentation Methods 0.000 claims description 45
- 230000004151 fermentation Effects 0.000 claims description 45
- 238000002156 mixing Methods 0.000 claims description 29
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 28
- 238000001035 drying Methods 0.000 claims description 28
- 238000001354 calcination Methods 0.000 claims description 22
- 239000000654 additive Substances 0.000 claims description 21
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 20
- 239000000047 product Substances 0.000 claims description 19
- AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Chemical compound OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 claims description 18
- 239000000706 filtrate Substances 0.000 claims description 18
- 230000000996 additive effect Effects 0.000 claims description 16
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 14
- 239000008367 deionised water Substances 0.000 claims description 14
- 229910021641 deionized water Inorganic materials 0.000 claims description 14
- 235000010413 sodium alginate Nutrition 0.000 claims description 14
- 239000000661 sodium alginate Substances 0.000 claims description 14
- 229940005550 sodium alginate Drugs 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 13
- 239000001506 calcium phosphate Substances 0.000 claims description 9
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 9
- 229940078499 tricalcium phosphate Drugs 0.000 claims description 9
- 229910000391 tricalcium phosphate Inorganic materials 0.000 claims description 9
- 235000019731 tricalcium phosphate Nutrition 0.000 claims description 9
- 229910052901 montmorillonite Inorganic materials 0.000 claims description 8
- 229920001661 Chitosan Polymers 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 7
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 7
- 238000002715 modification method Methods 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 7
- 238000002481 ethanol extraction Methods 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 4
- 208000016261 weight loss Diseases 0.000 claims 2
- 230000004580 weight loss Effects 0.000 claims 2
- 230000000694 effects Effects 0.000 abstract description 5
- 230000002195 synergetic effect Effects 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 9
- 244000075850 Avena orientalis Species 0.000 description 7
- 235000007319 Avena orientalis Nutrition 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 235000007558 Avena sp Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000021590 normal diet Nutrition 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention relates to the technical field of probiotic enzyme powder, and particularly discloses a preparation method of probiotic enzyme powder with a weight-losing function, which comprises the following steps: taking 20-30 parts of dendrobium, 10-15 parts of diet fruit, 2-6 parts of oat and 10-15 parts of probiotics; the lotus leaf leaching liquor and the modified montmorillonite are stirred and prepared according to the weight ratio of 4. The probiotic enzyme powder disclosed by the invention is prepared by fermenting dendrobium, diet fruit and oat through probiotics, and the lotus leaf leaching liquor and the modified montmorillonite are added to cooperate with each other to realize synergistic effect, so that the weight-losing efficiency of the product is enhanced, the raw materials are mutually cooperated, the weight-losing stability of the product is improved, the weight-losing rebound phenomenon is avoided, and the weight-losing effect is improved.
Description
Technical Field
The invention relates to the technical field of probiotic enzyme powder, and particularly relates to a preparation method and application of probiotic enzyme powder with a weight-losing function.
Background
Enzymes are old translations of enzymes. The human body is composed of cells, and each cell exhibits various vital activities due to the presence of enzymes, so that metabolism in the human body can be performed. The enzyme is a catalyst for metabolism in a human body, and various biochemical reactions can be carried out in the human body only if the enzyme exists. The more enzymes in the human body, the more complete the enzyme is, and the healthier the life is. When the human body does not contain active ferment, the life is greatly threatened. Human diseases are mostly related to the deficiency, utilization or synthesis disorder of enzymes, and enzyme powder supplement is one of the important contents in health care and health preservation at present.
The invention provides a preparation method and application of probiotic enzyme powder with a weight-losing function based on the fact that the existing probiotic enzyme powder does not have the weight-losing function, is low in weight-losing efficiency, is easy to rebound after weight-losing and is difficult to effectively lose weight.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a preparation method and application of probiotic enzyme powder with a weight-reducing function, so as to solve the problems in the background art.
The technical scheme adopted by the invention for solving the technical problem is as follows:
the invention provides a preparation method of probiotic enzyme powder with a weight-losing function, which comprises the following steps:
the method comprises the following steps: taking 20-30 parts of dendrobium, 10-15 parts of diet fruit, 2-6 parts of oat and 10-15 parts of probiotics;
step two: stirring and preparing the lotus leaf leaching liquor and the modified montmorillonite according to a weight ratio of 4;
step three: feeding the raw materials in the step one into a stirrer, stirring and mixing fully, then adding 5-10 parts of additives, and then feeding into a fermentation tank for fermentation treatment, and ending fermentation;
step four: and (4) after the fermentation is finished, separating the juice, and finally drying to obtain the probiotic ferment powder.
Preferably, the preparation method of the lotus leaf leaching liquor comprises the following steps:
s01: adding lotus leaves into 5-9 times of deionized water, then adding 10-20% of chitosan and 5-10% of sodium alginate solution of the total weight of the lotus leaves, and uniformly stirring;
s02: extracting with slow fire for 1-2 hr, and filtering to obtain filtrate;
s03: and (3) leaching the filter residue of the S02 by using 3-4 times of ethanol, and mixing the filter residue and the filtrate of the S02 to obtain a lotus leaf leaching solution after the leaching is finished.
Preferably, the pressure of ethanol extraction is 10-20MPa, and the extraction time is 35-45min.
Preferably, the mass fraction of the sodium alginate solution is 10-15%.
Preferably, the modification method of the modified montmorillonite is as follows:
s11: adding montmorillonite into a hydrochloric acid solution according to a weight ratio of 1;
s12: adding 1-3 parts of glycolic acid and 5-10 parts of citric acid into 15-20 parts of deionized water, uniformly stirring, then adding 2-4 parts of nano tricalcium phosphate, and continuously and fully mixing to obtain an additive;
s13: adding an additive accounting for 10-15% of the total weight of the S11 product into the S11, continuously mixing fully, and finally washing and drying;
s14: then the mixture is sent into a calcining furnace to be calcined for 10 to 20min, the calcining temperature is 350 to 400 ℃, and the mixture is cooled to the room temperature at the speed of 1 to 3 ℃/min after the calcining is finished.
Preferably, the mass fraction of the hydrochloric acid solution is 10-15%.
Preferably, the rotation speed of stirring preparation in the second step is 550-650r/min, and the stirring time is 20-30min.
Preferably, the temperature of the fermentation treatment in the fermentation tank is 32-35 ℃, and the fermentation time is 1-2 days.
Preferably, the third step is to stir and mix fully at the rotating speed of 1000-1500r/min for 20-30min; the drying temperature of the fourth step is 45-55 ℃, and the drying time is 1-2h.
The invention also provides application of the preparation method of the probiotic enzyme powder with the weight-losing function in weight losing.
Compared with the prior art, the invention has the following beneficial effects:
the probiotic enzyme powder disclosed by the invention is prepared by fermenting dendrobium, diet fruit and oat through probiotics, and the lotus leaf leaching liquor and the modified montmorillonite are added to cooperate with each other to realize synergistic effect, so that the weight-losing efficiency of the product is enhanced, the raw materials are mutually cooperated, the weight-losing stability of the product is improved, the weight-losing rebound phenomenon is avoided, and the weight-losing effect is improved.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The preparation method of the probiotic enzyme powder with the weight-reducing function in the embodiment includes the following steps:
the method comprises the following steps: taking 20-30 parts of dendrobium, 10-15 parts of diet fruit, 2-6 parts of oat and 10-15 parts of probiotics;
step two: stirring and preparing the lotus leaf leaching liquor and the modified montmorillonite according to a weight ratio of 4;
step three: feeding the raw materials in the step one into a stirrer, stirring and mixing fully, then adding 5-10 parts of additives, and then feeding into a fermentation tank for fermentation treatment, and ending fermentation;
step four: and (4) after the fermentation is finished, separating the juice, and finally drying to obtain the probiotic enzyme powder.
The preparation method of the lotus leaf leaching liquor of the embodiment comprises the following steps:
s01: adding lotus leaves into 5-9 times of deionized water, then adding 10-20% of chitosan and 5-10% of sodium alginate solution in the total amount of the lotus leaves, and uniformly stirring;
s02: extracting with slow fire for 1-2 hr, and filtering to obtain filtrate;
s03: and (3) leaching the filter residue of the S02 by using 3-4 times of ethanol, and merging the obtained product and the filtrate of the S02 to obtain a lotus leaf leaching liquor.
In this example, the pressure of ethanol extraction is 10-20MPa, and the extraction time is 35-45min.
The mass fraction of the sodium alginate solution in this example is 10-15%.
The modification method of the modified montmorillonite in the embodiment comprises the following steps:
s11: adding montmorillonite into a hydrochloric acid solution according to a weight ratio of 1;
s12: adding 1-3 parts of glycolic acid and 5-10 parts of citric acid into 15-20 parts of deionized water, uniformly stirring, then adding 2-4 parts of nano tricalcium phosphate, and continuously and fully mixing to obtain an additive;
s13: adding an additive accounting for 10-15% of the total weight of the S11 product into the S11, continuously and fully mixing, and finally washing and drying;
s14: then the mixture is sent into a calcining furnace to be calcined for 10 to 20min, the calcining temperature is 350 to 400 ℃, and the mixture is cooled to the room temperature at the speed of 1 to 3 ℃/min after the calcining is finished.
The mass fraction of the hydrochloric acid solution in this example is 10-15%.
In the second step of this embodiment, the rotation speed of the stirring is 550-650r/min, and the stirring time is 20-30min.
The temperature of the fermentation treatment in the fermentation tank of the embodiment is 32-35 ℃, and the fermentation time is 1-2 days.
In the third step of the present embodiment, the rotation speed of the stirring and mixing is 1000-1500r/min, and the stirring time is 20-30min; the drying temperature of the fourth step is 45-55 ℃, and the drying time is 1-2h.
The preparation method of the probiotic ferment powder with the weight-losing function is applied to weight losing.
Example 1.
The preparation method of the probiotic enzyme powder with the weight-reducing function in the embodiment includes the following steps:
the method comprises the following steps: taking 20 parts of dendrobium, 10 parts of weight-reducing fruits, 2 parts of oat and 10 parts of probiotics;
step two: stirring and preparing the lotus leaf leaching liquor and the modified montmorillonite according to a weight ratio of 4;
step three: feeding the raw materials in the step one into a stirrer, stirring and mixing fully, then adding 5 parts of additive, and then feeding into a fermentation tank for fermentation treatment, and ending fermentation;
step four: and (4) after the fermentation is finished, separating the juice, and finally drying to obtain the probiotic ferment powder.
The preparation method of the lotus leaf leaching liquor of the embodiment comprises the following steps:
s01: adding lotus leaves into 5 times of deionized water, then adding chitosan accounting for 10-20% of the total weight of the lotus leaves and a sodium alginate solution accounting for 5% of the total weight of the lotus leaves, and uniformly stirring;
s02: extracting with slow fire for 1 hr, and filtering to obtain filtrate;
s03: and (3) leaching the filter residue of the S02 by using 3 times of ethanol, obtaining the filtrate after the leaching is finished, and combining the filtrate with the filtrate of the S02 to obtain a lotus leaf leaching liquor.
The pressure of ethanol leaching in this example was 10MPa, and the leaching time was 35min.
The mass fraction of the sodium alginate solution in this example was 10%.
The modification method of the modified montmorillonite in the embodiment comprises the following steps:
s11: adding montmorillonite into a hydrochloric acid solution according to a weight ratio of 1;
s12: adding 1 part of glycolic acid and 5 parts of citric acid into 15 parts of deionized water, uniformly stirring, then adding 2 parts of nano tricalcium phosphate, and continuously and fully mixing to obtain an additive;
s13: adding an additive accounting for 10 percent of the total weight of the S11 product into the S11, continuously and fully mixing, and finally washing and drying;
s14: then the mixture is sent into a calcining furnace to be calcined for 10min, the calcining temperature is 350 ℃, and after the calcining is finished, the mixture is cooled to the room temperature at the speed of 1-3 ℃/min.
The mass fraction of the hydrochloric acid solution in this example was 10%.
In the second step of this embodiment, the rotation speed of the stirring is 550r/min, and the stirring time is 20min.
The temperature of the fermentation treatment in the fermenter in this example was 32 ℃ and the fermentation time was 1 day.
In the third step of the present embodiment, the rotation speed of the stirring and mixing is 1000r/min, and the stirring time is 20min; the temperature for drying in the fourth step is 45 ℃, and the drying time is 1h.
The preparation method of the probiotic enzyme powder with the weight-losing function disclosed by the embodiment is applied to weight losing.
Example 2.
The preparation method of the probiotic enzyme powder with the weight-reducing function in the embodiment includes the following steps:
the method comprises the following steps: taking 30 parts of dendrobium, 15 parts of weight-reducing fruits, 6 parts of oat and 15 parts of probiotics;
step two: stirring and preparing the lotus leaf leaching liquor and the modified montmorillonite according to the weight ratio of 4;
step three: feeding the raw materials in the step one into a stirrer, fully stirring and mixing, then adding 10 parts of additives, and then feeding into a fermentation tank for fermentation treatment, and ending fermentation;
step four: and (4) after the fermentation is finished, separating the juice, and finally drying to obtain the probiotic ferment powder.
The preparation method of the lotus leaf leaching liquor of the embodiment comprises the following steps:
s01: adding lotus leaves into 9 times of deionized water, then adding chitosan accounting for 20% of the total weight of the lotus leaves and sodium alginate solution accounting for 10% of the total weight of the lotus leaves, and uniformly stirring;
s02: extracting with slow fire for 2 hr, and filtering to obtain filtrate;
s03: and (3) leaching the filter residue of the S02 by using 4 times of ethanol, and merging the obtained product and the filtrate of the S02 to obtain a lotus leaf leaching liquor.
The pressure of ethanol leaching in this example was 10MPa, and the leaching time was 35min.
The mass fraction of the sodium alginate solution in this example was 10%.
The modification method of the modified montmorillonite in the embodiment comprises the following steps:
s11: adding montmorillonite into a hydrochloric acid solution according to a weight ratio of 1;
s12: adding 3 parts of glycolic acid and 5 parts of citric acid into 20 parts of deionized water, uniformly stirring, then adding 4 parts of nano tricalcium phosphate, and continuously and fully mixing to obtain an additive;
s13: adding an additive accounting for 15 percent of the total weight of the S11 product into the S11, continuously and fully mixing, and finally washing and drying;
s14: and then, feeding the mixture into a calcining furnace to calcine for 20min, wherein the calcining temperature is 400 ℃, and after the calcining is finished, the mixture is cooled to room temperature at the speed of 3 ℃/min.
The mass fraction of the hydrochloric acid solution in this example was 15%.
In the second step of this embodiment, the rotation speed of the stirring is 650r/min, and the stirring time is 30min.
The temperature of the fermentation treatment in the fermenter in this example was 35 ℃ and the fermentation time was 2 days.
In the third step of the present embodiment, the rotation speed of the stirring and mixing is 1500r/min, and the stirring time is 30min; the temperature for drying in the fourth step is 55 ℃, and the drying time is 2h.
The preparation method of the probiotic enzyme powder with the weight-losing function disclosed by the embodiment is applied to weight losing.
Example 3.
The preparation method of the probiotic ferment powder with the weight-losing function includes the following steps:
the method comprises the following steps: taking 25 parts of dendrobium, 12.5 parts of weight-losing fruits, 4 parts of oats and 12.5 parts of probiotics;
step two: stirring and preparing the lotus leaf leaching liquor and the modified montmorillonite according to a weight ratio of 4;
step three: feeding the raw materials in the step one into a stirrer, fully stirring and mixing, then adding 7.5 parts of additives, and then feeding into a fermentation tank for fermentation treatment, and finishing fermentation;
step four: and (4) after the fermentation is finished, separating the juice, and finally drying to obtain the probiotic enzyme powder.
The preparation method of the lotus leaf leaching liquor of the embodiment comprises the following steps:
s01: adding lotus leaves into deionized water 7 times, then adding chitosan 15% of the total weight of the lotus leaves and sodium alginate solution 7.5%, and stirring uniformly;
s02: extracting with slow fire for 1.5 hr, and filtering to obtain filtrate;
s03: and (3) leaching the filter residue of the S02 by using 3.5 times of ethanol, and merging the obtained product and the filtrate of the S02 to obtain a lotus leaf leaching liquor.
The pressure of ethanol extraction in this example was 15MPa, and the extraction time was 40min.
The mass fraction of the sodium alginate solution in this example was 12.5%.
The modification method of the modified montmorillonite in the embodiment comprises the following steps:
s11: adding montmorillonite into a hydrochloric acid solution according to a weight ratio of 1;
s12: adding 2 parts of glycolic acid and 7.5 parts of citric acid into 17.5 parts of deionized water, uniformly stirring, then adding 3 parts of nano tricalcium phosphate, and continuously and fully mixing to obtain an additive;
s13: adding an additive accounting for 12.5 percent of the total weight of the S11 product into the S11, continuously and fully mixing, and finally washing and drying;
s14: then the mixture is sent into a calcining furnace to be calcined for 15min, the calcining temperature is 375 ℃, and the temperature is reduced to the room temperature at the speed of 2 ℃/min after the calcining is finished.
The mass fraction of the hydrochloric acid solution in this example was 12.5%.
In the second step of this embodiment, the rotation speed of the stirring preparation is 600r/min, and the stirring time is 25min.
The temperature of the fermentation treatment in the fermenter in this example was 33.5 ℃ and the fermentation time was 1.5 days.
In the third step of the present embodiment, the rotation speed of the stirring and mixing is 1250r/min, and the stirring time is 25min; the temperature for drying in the fourth step is 50 ℃, and the drying time is 1.5h.
The preparation method of the probiotic enzyme powder with the weight-losing function disclosed by the embodiment is applied to weight losing.
Example 4.
The preparation method of the probiotic ferment powder with the weight-losing function includes the following steps:
the method comprises the following steps: taking 22 parts of dendrobium, 12 parts of weight-reducing fruits, 3 parts of oat and 12 parts of probiotics;
step two: stirring and preparing the lotus leaf leaching liquor and the modified montmorillonite according to the weight ratio of 4;
step three: feeding the raw materials in the step one into a stirrer, stirring and mixing fully, then adding 6 parts of additive, and then feeding into a fermentation tank for fermentation treatment, and ending fermentation;
step four: and (4) after the fermentation is finished, separating the juice, and finally drying to obtain the probiotic ferment powder.
The preparation method of the lotus leaf leaching liquor of the embodiment comprises the following steps:
s01: adding lotus leaves into deionized water 6 times, then adding chitosan 12% of the total weight of the lotus leaves and sodium alginate solution 6%, and stirring uniformly;
s02: extracting with slow fire for 1.2 hr, and filtering to obtain filtrate;
s03: and (3) leaching the filter residue of the S02 by using 3.2 times of ethanol, obtaining the filtrate after the leaching is finished, and combining the filtrate with the filtrate of the S02 to obtain a lotus leaf leaching liquor.
The pressure of ethanol leaching in this example was 12MPa, and the leaching time was 37min.
The mass fraction of the sodium alginate solution in this example was 12%.
The modification method of the modified montmorillonite in the embodiment comprises the following steps:
s11: adding montmorillonite into a hydrochloric acid solution according to a weight ratio of 1;
s12: adding 2 parts of glycolic acid and 6 parts of citric acid into 16 parts of deionized water, uniformly stirring, then adding 3 parts of nano tricalcium phosphate, and continuously and fully mixing to obtain an additive;
s13: adding an additive accounting for 12 percent of the total weight of the S11 product into the S11, continuously and fully mixing, and finally washing and drying;
s14: then the mixture is sent into a calcining furnace to be calcined for 12min, the calcining temperature is 360 ℃, and the temperature is reduced to the room temperature at the speed of 2 ℃/min after the calcining is finished.
The mass fraction of the hydrochloric acid solution in this example was 12%.
In the second step of this embodiment, the rotation speed of the stirring preparation is 560r/min, and the stirring time is 22min.
The temperature of the fermentation treatment in the fermenter in this example was 33 ℃ and the fermentation time was 1.2 days.
In the third step of the present embodiment, the rotation speed of the stirring and mixing is 1200r/min, and the stirring time is 22min; the temperature for drying in the fourth step is 47 ℃, and the drying time is 1.2h.
The preparation method of the probiotic enzyme powder with the weight-losing function disclosed by the embodiment is applied to weight losing.
Comparative example 1.
Unlike example 3, no lotus leaf leach liquor was added.
Comparative example 2.
The difference from example 3 is that no modified montmorillonite was added.
Comparative example 3.
The difference from example 3 is that the modified montmorillonite is replaced by montmorillonite.
Comparative example 4.
The difference from the example 3 is that the nanometer tricalcium phosphate is not added in the preparation of the modified montmorillonite.
Comparative example 5.
The difference from example 3 is that the modified montmorillonite was not calcined in its preparation.
Comparative example 6.
The difference from example 3 is that glycolic acid is not added in the modification of the modified montmorillonite.
The products of examples 1-4 and comparative examples 1-6 of the invention are taken with normal diet and proper exercise, and are forbidden to drink violent food and high-calorie food. 1 bag each time, 10g each bag; after 1 month of administration, recording the weight change, relative to the weight change of the product not administered, testing the weight change every other week, relative to the weight change after 1 month of administration;
the products of examples 1 to 4 and comparative examples 1 to 6 were subjected to a performance test;
as can be seen from comparative examples 1 to 6 and examples 1 to 4; the lotus leaf leaching liquor and the lotus leaf leaching liquor are not added, the weight-losing effect of the product is remarkably deteriorated, the product is easy to rebound after losing weight, in addition, the nano tricalcium phosphate is not added in the preparation of the modified montmorillonite, the calcination treatment and the glycolic acid are not added, the performance deterioration trend of the product is remarkable, only the lotus leaf leaching liquor prepared by the method can remarkably enhance the weight-losing effect and the stability of the product after losing weight, and the weight-losing rebound is avoided.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (10)
1. The preparation method of the probiotic enzyme powder with the weight-losing function is characterized by comprising the following steps of:
the method comprises the following steps: taking 20-30 parts of dendrobium, 10-15 parts of diet fruit, 2-6 parts of oat and 10-15 parts of probiotics;
step two: stirring and preparing the lotus leaf leaching liquor and the modified montmorillonite according to a weight ratio of 4;
step three: feeding the raw materials in the step one into a stirrer, stirring and mixing fully, then adding 5-10 parts of additives, and then feeding into a fermentation tank for fermentation treatment, and ending fermentation;
step four: and (4) after the fermentation is finished, separating the juice, and finally drying to obtain the probiotic enzyme powder.
2. The method for preparing probiotic ferment powder with weight-losing function according to claim 1, wherein the preparation method of the lotus leaf leaching solution comprises the following steps:
s01: adding lotus leaves into 5-9 times of deionized water, then adding 10-20% of chitosan and 5-10% of sodium alginate solution of the total weight of the lotus leaves, and uniformly stirring;
s02: extracting with slow fire for 1-2 hr, and filtering to obtain filtrate;
s03: and (3) leaching the filter residue of the S02 by using 3-4 times of ethanol, and mixing the filter residue and the filtrate of the S02 to obtain a lotus leaf leaching solution after the leaching is finished.
3. The method for preparing the probiotic ferment powder with the weight-losing function according to claim 2, wherein the pressure of ethanol extraction is 10-20MPa, and the extraction time is 35-45min.
4. The preparation method of the probiotic enzyme powder with the weight-losing function according to claim 2, wherein the mass fraction of the sodium alginate solution is 10-15%.
5. The method for preparing probiotic ferment powder with weight-losing function according to claim 1, wherein the modification method of the modified montmorillonite is as follows:
s11: adding montmorillonite into a hydrochloric acid solution according to a weight ratio of 1;
s12: adding 1-3 parts of glycolic acid and 5-10 parts of citric acid into 15-20 parts of deionized water, uniformly stirring, then adding 2-4 parts of nano tricalcium phosphate, and continuously and fully mixing to obtain an additive;
s13: adding an additive accounting for 10-15% of the total weight of the S11 product into the S11, continuously and fully mixing, and finally washing and drying;
s14: then the mixture is sent into a calcining furnace to be calcined for 10 to 20min, the calcining temperature is 350 to 400 ℃, and the mixture is cooled to the room temperature at the speed of 1 to 3 ℃/min after the calcining is finished.
6. The preparation method of the probiotic ferment powder with the weight-losing function according to claim 5, wherein the mass fraction of the hydrochloric acid solution is 10-15%.
7. The method for preparing probiotic ferment powder with weight-losing function according to claim 1, wherein the rotation speed of the stirring preparation in the second step is 550-650r/min, and the stirring time is 20-30min.
8. The method for preparing probiotic ferment powder with weight-losing function according to claim 1, wherein the fermentation treatment temperature in the fermentation tank is 32-35 ℃, and the fermentation time is 1-2 days.
9. The preparation method of the probiotic ferment powder with the weight-losing function according to claim 1, wherein the stirring and mixing in the third step are carried out at a sufficient rotation speed of 1000-1500r/min for 20-30min; the drying temperature of the fourth step is 45-55 ℃, and the drying time is 1-2h.
10. Use of the probiotic ferment powder with weight-loss function according to any one of claims 1 to 9 in weight loss.
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Application publication date: 20221129 |