CN115381744A - Ganoderma lucidum fermentation liquor composition and application thereof in anti-aging and moisturizing skin care products - Google Patents
Ganoderma lucidum fermentation liquor composition and application thereof in anti-aging and moisturizing skin care products Download PDFInfo
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- CN115381744A CN115381744A CN202111173802.9A CN202111173802A CN115381744A CN 115381744 A CN115381744 A CN 115381744A CN 202111173802 A CN202111173802 A CN 202111173802A CN 115381744 A CN115381744 A CN 115381744A
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- Prior art keywords
- ganoderma lucidum
- moisturizing
- lucidum fermentation
- fermentation broth
- composition
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- 240000008397 Ganoderma lucidum Species 0.000 title claims abstract description 68
- 235000001637 Ganoderma lucidum Nutrition 0.000 title claims abstract description 68
- 238000000855 fermentation Methods 0.000 title claims abstract description 52
- 230000004151 fermentation Effects 0.000 title claims abstract description 52
- 239000000203 mixture Substances 0.000 title claims abstract description 47
- 230000003020 moisturizing effect Effects 0.000 title claims abstract description 32
- 230000003712 anti-aging effect Effects 0.000 title claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 19
- 239000004480 active ingredient Substances 0.000 claims abstract description 18
- 239000003349 gelling agent Substances 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 27
- -1 polysaccharide compounds Chemical class 0.000 claims description 22
- 229920001282 polysaccharide Polymers 0.000 claims description 20
- 239000005017 polysaccharide Substances 0.000 claims description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 19
- 229920002385 Sodium hyaluronate Polymers 0.000 claims description 18
- 229940010747 sodium hyaluronate Drugs 0.000 claims description 18
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims description 18
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 12
- 229920002498 Beta-glucan Polymers 0.000 claims description 12
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 12
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 12
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 claims description 11
- MQFYRUGXOJAUQK-UHFFFAOYSA-N 2-[2-[2-(2-octadecanoyloxyethoxy)ethoxy]ethoxy]ethyl octadecanoate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCCOCCOCCOCCOC(=O)CCCCCCCCCCCCCCCCC MQFYRUGXOJAUQK-UHFFFAOYSA-N 0.000 claims description 9
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 8
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 claims description 8
- 229910052901 montmorillonite Inorganic materials 0.000 claims description 8
- 150000003384 small molecules Chemical class 0.000 claims description 8
- 239000000600 sorbitol Substances 0.000 claims description 8
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims description 7
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 4
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 claims description 2
- 229920002907 Guar gum Polymers 0.000 claims description 2
- 239000000665 guar gum Substances 0.000 claims description 2
- 229960002154 guar gum Drugs 0.000 claims description 2
- 235000010417 guar gum Nutrition 0.000 claims description 2
- 239000011780 sodium chloride Substances 0.000 claims description 2
- 241000222336 Ganoderma Species 0.000 abstract description 15
- 230000000694 effects Effects 0.000 abstract description 6
- 230000035699 permeability Effects 0.000 abstract description 3
- 230000002195 synergetic effect Effects 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 description 19
- 238000012258 culturing Methods 0.000 description 14
- 239000003921 oil Substances 0.000 description 14
- 235000019198 oils Nutrition 0.000 description 14
- 229920001817 Agar Polymers 0.000 description 10
- 239000008272 agar Substances 0.000 description 10
- 239000000047 product Substances 0.000 description 10
- 239000000843 powder Substances 0.000 description 8
- 229910019142 PO4 Inorganic materials 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 description 6
- 238000010899 nucleation Methods 0.000 description 6
- 239000010452 phosphate Substances 0.000 description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 241001506047 Tremella Species 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 150000004676 glycans Chemical class 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000009423 ventilation Methods 0.000 description 4
- 230000036571 hydration Effects 0.000 description 3
- 238000006703 hydration reaction Methods 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 229920002261 Corn starch Polymers 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 229930003270 Vitamin B Natural products 0.000 description 2
- 239000008120 corn starch Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 2
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 235000012424 soybean oil Nutrition 0.000 description 2
- 239000003549 soybean oil Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 238000000108 ultra-filtration Methods 0.000 description 2
- 235000019156 vitamin B Nutrition 0.000 description 2
- 239000011720 vitamin B Substances 0.000 description 2
- TWCMVXMQHSVIOJ-UHFFFAOYSA-N Aglycone of yadanzioside D Natural products COC(=O)C12OCC34C(CC5C(=CC(O)C(O)C5(C)C3C(O)C1O)C)OC(=O)C(OC(=O)C)C24 TWCMVXMQHSVIOJ-UHFFFAOYSA-N 0.000 description 1
- PLMKQQMDOMTZGG-UHFFFAOYSA-N Astrantiagenin E-methylester Natural products CC12CCC(O)C(C)(CO)C1CCC1(C)C2CC=C2C3CC(C)(C)CCC3(C(=O)OC)CCC21C PLMKQQMDOMTZGG-UHFFFAOYSA-N 0.000 description 1
- 235000010254 Jasminum officinale Nutrition 0.000 description 1
- 240000005385 Jasminum sambac Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001153 anti-wrinkle effect Effects 0.000 description 1
- 229910052788 barium Inorganic materials 0.000 description 1
- DSAJWYNOEDNPEQ-UHFFFAOYSA-N barium atom Chemical compound [Ba] DSAJWYNOEDNPEQ-UHFFFAOYSA-N 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- PFOARMALXZGCHY-UHFFFAOYSA-N homoegonol Natural products C1=C(OC)C(OC)=CC=C1C1=CC2=CC(CCCO)=CC(OC)=C2O1 PFOARMALXZGCHY-UHFFFAOYSA-N 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- GOMNOOKGLZYEJT-UHFFFAOYSA-N isoflavone Chemical compound C=1OC2=CC=CC=C2C(=O)C=1C1=CC=CC=C1 GOMNOOKGLZYEJT-UHFFFAOYSA-N 0.000 description 1
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 description 1
- 235000008696 isoflavones Nutrition 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000037394 skin elasticity Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/20—Halogens; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/26—Aluminium; Compounds thereof
-
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/737—Galactomannans, e.g. guar; Derivatives thereof
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- A—HUMAN NECESSITIES
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/86—Polyethers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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- A61Q19/08—Anti-ageing preparations
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- A—HUMAN NECESSITIES
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
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- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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- Health & Medical Sciences (AREA)
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- Engineering & Computer Science (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Inorganic Chemistry (AREA)
- Organic Chemistry (AREA)
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- Dermatology (AREA)
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- Genetics & Genomics (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Emergency Medicine (AREA)
- Cosmetics (AREA)
Abstract
The invention provides a lucid ganoderma fermentation liquid composition and application thereof in anti-aging and moisturizing skin care products, wherein the lucid ganoderma fermentation liquid composition comprises, by weight, 3-7 parts of active ingredients, 0.3-1 part of gelling agent, 4-8 parts of moisturizing factor and 45-70 parts of water, and the active ingredients comprise lucid ganoderma spore oil and/or lucid ganoderma fermentation liquid. The composition which is prepared by selecting the ganoderma lucidum extract and the ganoderma lucidum spore oil as active ingredients and other ingredients in a specific ratio not only has high and low temperature resistance stability, but also has excellent moisturizing effect and the effects of moisturizing and locking water through the mutual synergistic effect of the special moisturizing factors and the gelling agent, and the skin care product prepared by the composition is fresh and cool in texture, is not sticky and has good air permeability.
Description
Technical Field
The invention relates to the technical field of skin care products, in particular to an IPC classification A61K8/73, and more particularly relates to a ganoderma lucidum fermentation liquor composition and application thereof in an anti-aging and moisturizing skin care product.
Background
The ganoderma lucidum extract has a certain skin care effect, but the types of skin care products containing the ganoderma lucidum extract on the market are very few, and under the condition, the applicant previously develops a composition containing the ganoderma lucidum extract and an application of the composition in a moisturizing and anti-wrinkle cosmetic, and the composition disclosed in the patent CN111135122A can improve the skin elasticity, the skin darkness and the fine lines, and also has higher heat and cold resistance stability, but after a period of use, the product is found to be thicker, airtight and have a certain sense of stickiness after being smeared, and the moisturizing and water locking effects are weaker, so the applicant further improves and optimizes the composition, and particularly puts forward the application.
Disclosure of Invention
In order to solve the technical problems, the invention provides a ganoderma lucidum fermentation liquid composition which comprises, by weight, 3-7 parts of active ingredients, 0.3-1 part of a gelling agent, 4-8 parts of a moisturizing factor and 45-70 parts of water, wherein the active ingredients comprise ganoderma lucidum spore oil and/or ganoderma lucidum fermentation liquid.
Preferably, the active ingredients include ganoderma spore oil and ganoderma fermentation broth.
In some embodiments, the weight ratio of the ganoderma spore oil to the ganoderma lucidum fermentation broth is (0.2-0.4): 1.
preferably, the weight ratio of the ganoderma lucidum spore oil to the ganoderma lucidum fermentation liquid is 0.3:1.
in some embodiments, the method for preparing the ganoderma lucidum fermentation liquid comprises the following steps:
the preparation of the ganoderma lucidum fermentation liquor comprises the following steps:
(1) Culturing the Tibetan Ganoderma I slant strain in culture medium at 25-26 deg.C for 25 days to obtain cultured Ganoderma strain;
(2) Keeping the temperature at 25-26 ℃, adding a ganoderma lucidum strain culture medium without agar into a triangular flask, then carrying out high-temperature sterilization at 121 ℃ for 30min, inoculating the ganoderma lucidum strain cultured in the step (1) into the strain culture medium in the triangular flask, then placing on an oscillator for culturing for 6 days, controlling the oscillation speed at 150rpm and the temperature at 25-26 ℃, and obtaining shaking table seeds;
(3) Seed tank culture: maintaining the temperature at 25-26 deg.C, sucking shake culture seeds into 300L seeding tank culture medium under negative pressure, culturing for 4 days with stirring speed of 120r/min and ventilation rate of 0.5vvm;
(4) Culturing in a propagation tank: maintaining the temperature at 25-26 deg.C, sucking the culture in seeding tank into 1.5t propagation tank under negative pressure, culturing for 3 days with stirring speed of 120r/min and ventilation amount of 0.5vvm;
(5) Culturing in a fermentation tank: keeping the temperature at 25-26 ℃, sucking the culture in the propagation tank into a fermentation tank of 7t under negative pressure, culturing for 3 days, stirring at 120r/min, and ventilating at 0.5vvm;
(6) And (3) coarsely filtering the substances in the fermentation tank by using an ultrafiltration membrane to obtain the ganoderma lucidum fermentation liquor.
Wherein the Tibetan Ganoderma lucidum I strain is a Ganoderma lucidum strain which is cultivated by Shanghai standing grain to health science and technology development Limited company and Tibet autonomous region agricultural academy of sciences.
The formula of the culture medium in the ganoderma lucidum strain culture comprises the following components: 2g of peptone, 20g of glucose, 1.4g of phosphate compound, 0.5g of magnesium sulfate heptahydrate, 10mg of vitamin B, 18g of agar and 1000ml of water.
The phosphate compound comprises dipotassium hydrogen phosphate and potassium dihydrogen phosphate, and the weight ratio of the phosphate compound to the potassium dihydrogen phosphate is 1:0.4.
the protein peptide was purchased from Sichuan Baoxin Biotechnology Co.
The Agar is purchased from Hangzhou Mirabachi Biotech Co., ltd and is named as Agar powder Agar.
The formula of the seeding tank culture medium comprises, by mass, 2% of corn starch, 1.5% of glucose, 0.1% of potassium dihydrogen phosphate, 0.05% of magnesium sulfate, 0.2% of yeast powder, 0.05% of soybean oil and the balance of water.
The yeast powder is Angel yeast powder YP600.
The culture medium formula of the propagation tank is the same as that of the seed tank.
The formula of the culture medium cultured in the fermentation tank is the same as that of the culture medium cultured in the propagation tank.
In some embodiments, the moisturizing factor comprises at least one of sodium hyaluronate, a polysaccharide compound, a small molecule alcohol.
Preferably, the moisturizing factor comprises a mixture of sodium hyaluronate, polysaccharide compound and small molecule alcohol.
More preferably, the weight ratio of the sodium hyaluronate, the polysaccharide compound and the small molecule alcohol is 1: (0.8-1.2): (0.5-0.9).
More preferably, the weight ratio of the sodium hyaluronate, the polysaccharide compound and the small molecule alcohol is 1:1:0.7.
in some embodiments, the sodium hyaluronate has a molecular weight of 100 to 180 ten thousand.
Preferably, the sodium hyaluronate is purchased from western safe auspicious biotechnology limited.
In some embodiments, the small molecule alcohol comprises at least one of glycerol, butylene glycol, propylene glycol, sorbitol.
Preferably, the small molecule alcohol comprises a mixture of glycerol and sorbitol in a weight ratio of 1: (0.8-1.2).
More preferably, the small molecule alcohol comprises a mixture of glycerol and sorbitol in a weight ratio of 1:1.
in some embodiments, the polysaccharide compound comprises beta glucan and/or trehalose.
Preferably, the polysaccharide compounds include beta glucan and trehalose.
In some embodiments, the weight ratio of beta glucan to trehalose is (0.5-1): 1.
preferably, the weight ratio of beta glucan to trehalose is 0.75:1.
in some embodiments, the gelling agent comprises at least one of guar gum, sodium chloride, montmorillonite, PEG-150 distearate.
Preferably, the gelling agent comprises montmorillonite and PEG-150 distearate in a weight ratio of (0.6-0.9): 1.
more preferably, the gelling agent comprises montmorillonite and PEG-150 distearate in a weight ratio of 0.75:1.
the applicant finds that the previously developed composition containing the ganoderma lucidum extract has a heavy sticky feeling and relatively weak effects of air-tightness, moisture retention and water locking when used in a skin care product, and supposes that the composition is probably because tremella polysaccharide with the molecular weight of more than 100 ten thousand is adopted, the viscosity of the tremella polysaccharide is high, so that the tremella polysaccharide has a heavy sticky feeling, and after a large amount of research, the applicant finds that the composition which is formed by selecting ganoderma lucidum spore oil and ganoderma lucidum fermentation liquor as active ingredients, a moisturizing factor and a gelling agent in a specific ratio has excellent anti-aging effect, and has good water-tightness, moisture retention and good moisture retention effects, and is fresh and non-greasy in texture and good in air permeability.
The invention also provides application of the ganoderma lucidum fermentation liquid composition in anti-aging and moisturizing skin care products.
Has the advantages that:
(1) According to the invention, the composition which is composed of the ganoderma lucidum spore oil and the ganoderma lucidum fermentation liquor as active ingredients, the moisturizing factor and the gelling agent in a specific ratio has excellent anti-aging effect, and also has good effects of moisturizing, water locking and moisturizing, and meanwhile, the texture is fresh and cool, and the composition is not greasy and has good air permeability;
(2) According to the invention, fatty acid substances in the ganoderma lucidum spore oil and high-content isoflavone aglycone in the ganoderma lucidum fermentation liquor in a specific proportion are adopted to generate a good synergistic effect, so that the ganoderma lucidum spore oil has good functions of resisting oxidation and removing free radicals;
(3) The gelling agent PEG-150 distearate used in the system can form a three-dimensional hydration network, and the montmorillonite swells along with the progress of hydration and is crosslinked into a thickening structure through the attraction of positive and negative charges in the system, so that the stability of the composition is improved, meanwhile, sodium hyaluronate and small molecular alcohol are added into the system and can easily permeate into the cuticle of the skin, and the hydroxyl on a sugar unit in the polysaccharide compound can also form the three-dimensional hydration network structure through the action of water molecules, so that the moisturizing factor can be locked in the cuticle of the skin for a long time, and the long-acting moisturizing effect is achieved.
Detailed Description
Example 1
The ganoderma lucidum fermentation liquid composition comprises, by weight, 5 parts of active ingredients, 0.65 part of a gelling agent, 6 parts of a moisturizing factor and 56 parts of water, wherein the active ingredients comprise ganoderma lucidum spore oil and ganoderma lucidum fermentation liquid, and the weight ratio of the active ingredients to the ganoderma lucidum fermentation liquid is 0.3:1.
the moisturizing factor is a mixture of sodium hyaluronate, polysaccharide compound and micromolecular alcohol, and the weight ratio is 1:1:0.7.
the molecular weight of the sodium hyaluronate is 100-180 ten thousand, and the sodium hyaluronate is purchased from western safe Olympic Biotechnology Limited company.
The polysaccharide compound is beta glucan and trehalose, and the weight ratio of the beta glucan to the trehalose is 0.75:1.
the micromolecular alcohol is a mixture of glycerol and sorbitol, and the weight ratio of the micromolecular alcohol to the glycerol is 1:1.
the gelling agent is montmorillonite and PEG-150 distearate, the weight ratio is 0.75:1.
the ganoderma spore oil is purchased from Feng Baokang source biotechnology limited in salt city.
The preparation of the ganoderma lucidum fermentation liquor comprises the following steps:
(1) Culturing the slant strain of Tibetan Ganoderma at 25-26 deg.C in culture medium for 25 days to obtain cultured Ganoderma strain;
(2) Keeping the temperature at 25-26 ℃, adding a ganoderma lucidum strain culture medium without agar into a triangular flask, then carrying out high-temperature sterilization at 121 ℃ for 30min, inoculating the ganoderma lucidum strain cultured in the step (1) into the strain culture medium in the triangular flask, then placing the strain culture medium on an oscillator for culturing for 6 days, controlling the oscillation speed at 150rpm and the temperature at 25-26 ℃ to obtain shaking table seeds;
(3) Seed tank culture: maintaining the temperature at 25-26 deg.C, sucking shake culture seeds into 300L seeding tank culture medium under negative pressure, culturing for 4 days with stirring speed of 120r/min and ventilation rate of 0.5vvm;
(4) Culturing in a propagation tank: maintaining the temperature at 25-26 deg.C, sucking the culture in seeding tank into 1.5t propagation tank under negative pressure, culturing for 3 days with stirring speed of 120r/min and ventilation amount of 0.5vvm;
(5) Culturing in a fermentation tank: keeping the temperature at 25-26 ℃, sucking the culture in the propagation tank into a fermentation tank of 7t under negative pressure, culturing for 3 days, stirring at 120r/min, and ventilating at 0.5vvm;
(6) And (3) coarsely filtering the substances in the fermentation tank by using an ultrafiltration membrane to obtain the ganoderma lucidum fermentation liquor.
Wherein the Tibetan Ganoderma lucidum I strain is a Ganoderma lucidum strain which is cultivated by Shanghai standing grain to health science and technology development Limited company and Tibet autonomous region agricultural academy of sciences.
The Ganoderma strain in culture the formula of the culture medium comprises: 2g of peptone, 20g of glucose, 1.4g of phosphate compound, 0.5g of magnesium sulfate heptahydrate, 10mg of vitamin B, 18g of agar and 1000ml of water.
The phosphate compound comprises dipotassium hydrogen phosphate and potassium dihydrogen phosphate, and the weight ratio of the phosphate compound to the potassium dihydrogen phosphate is 1:0.4.
the protein peptide is purchased from Sichuan Baoxin Biotechnology Limited.
The Agar is purchased from Hangzhou jasmine barium special biological science and technology limited company and is named as Agar powder Agar.
The formula of the seeding tank culture medium comprises, by mass, 2% of corn starch, 1.5% of glucose, 0.1% of potassium dihydrogen phosphate, 0.05% of magnesium sulfate, 0.2% of yeast powder, 0.05% of soybean oil and the balance of water.
The yeast powder is Angel yeast powder YP600.
The culture medium formula of the propagation tank is the same as that of the seed tank.
The formula of the culture medium cultured in the fermentation tank is the same as that of the culture medium cultured in the propagation tank.
The composition can be used for preparing anti-aging and moisturizing skin care products.
Example 2
The ganoderma lucidum fermentation liquid composition comprises, by weight, 3 parts of active ingredients, 0.3 part of a gelling agent, 4 parts of a moisturizing factor and 45 parts of water, wherein the active ingredients comprise ganoderma lucidum spore oil and ganoderma lucidum fermentation liquid, and the weight ratio of the active ingredients to the ganoderma lucidum fermentation liquid is 0.2:1.
the moisturizing factor is a mixture of sodium hyaluronate, polysaccharide compound and micromolecular alcohol, and the weight ratio is 1:0.8:0.5.
the molecular weight of the sodium hyaluronate is 100-180 ten thousand, and the sodium hyaluronate is purchased from western safe Olympic Biotechnology Limited company.
The polysaccharide compound is beta glucan and trehalose, and the weight ratio of the beta glucan to the trehalose is 0.5:1.
the micromolecular alcohol is a mixture of glycerol and sorbitol, and the weight ratio of the micromolecular alcohol to the glycerol is 1:0.8.
the gelling agent is montmorillonite and PEG-150 distearate, and the weight ratio is 0.6:1.
the ganoderma spore oil is purchased from Feng Baokang source biotechnology limited in salt city.
The specific preparation method of the ganoderma lucidum fermentation liquid is the same as that of the example 1.
The composition can be used for preparing anti-aging and moisturizing skin care products.
Example 3
The ganoderma lucidum fermentation liquid composition comprises 7 parts of active ingredients, 1 part of gelling agent, 8 parts of moisturizing factor and 70 parts of water by weight, wherein the active ingredients comprise ganoderma lucidum spore oil and ganoderma lucidum fermentation liquid, and the weight ratio is 0.4:1.
the moisturizing factor is a mixture of sodium hyaluronate, polysaccharide compound and micromolecular alcohol, and the weight ratio is 1:1.2:0.9.
the molecular weight of the sodium hyaluronate is 100-180 ten thousand, and the sodium hyaluronate is purchased from western safe Olympic Biotechnology Limited company.
The polysaccharide compound is beta glucan and trehalose, and the weight ratio of the beta glucan to the trehalose is 1:1.
the micromolecular alcohol is a mixture of glycerol and sorbitol, and the weight ratio of the micromolecular alcohol to the sorbitol is 1:1.2.
the gelling agent is montmorillonite and PEG-150 distearate, and the weight ratio is 0.9:1.
the ganoderma spore oil is purchased from Feng Baokang source biotechnology limited in salt city.
The specific preparation method of the ganoderma lucidum fermentation liquor is the same as that in the embodiment 1.
The composition can be used for preparing anti-aging and moisturizing skin care products.
Example 4
The embodiment of the present invention provides a ganoderma lucidum fermentation broth composition, which is the same as in embodiment 1, except that the polysaccharide compound is tremella polysaccharide.
Example 5
This example provides a Ganoderma lucidum fermentation broth composition, in accordance with the example 1, except that the gelling agent does not include PEG-150 distearate.
Performance testing
1. Evaluation of sticky feeling
Randomly selecting 10 subjects, respectively smearing the compositions prepared in examples 1-5 on the backs of hands, patting lightly to absorb, and scoring the sticky feeling; wherein the refreshing, non-sticky and slightly sticky notes are divided into 4 to 5 points, the slight sticky notes are divided into 2 to 3 points, the sticky notes are divided into 0 to 1 point obviously, the total scoring of 10 persons is summarized, and the results are recorded in the table 1.
TABLE 1
| Numbering | Evaluation of sticky feeling |
| Example 1 | 46 minutes |
| Example 2 | 40 minutes |
| Example 3 | 42 points of |
| Example 4 | 35 minutes (min.) |
| Example 5 | 38 points (B) |
Claims (10)
1. The ganoderma lucidum fermentation liquid composition is characterized by comprising 3-7 parts of active ingredients, 0.3-1 part of gelling agent, 4-8 parts of moisturizing factor and 45-70 parts of water in parts by weight, wherein the active ingredients comprise ganoderma lucidum spore oil and/or ganoderma lucidum fermentation liquid.
2. The ganoderma lucidum fermentation broth composition according to claim 1, wherein the weight ratio of ganoderma lucidum spore oil to ganoderma lucidum fermentation broth is (0.2-0.4): 1.
3. the ganoderma lucidum fermentation broth composition of claim 2, wherein the weight ratio of ganoderma lucidum spore oil to ganoderma lucidum fermentation broth is 0.3:1.
4. the ganoderma lucidum fermentation liquor composition as claimed in claim 1, wherein the moisturizing factor comprises at least one of sodium hyaluronate, polysaccharide compounds and small molecular alcohol.
5. The ganoderma lucidum fermentation broth composition according to claim 4, wherein the molecular weight of the sodium hyaluronate is 100-180 ten thousand.
6. The ganoderma lucidum fermentation broth composition according to claim 4, wherein the small molecule alcohol comprises at least one of glycerol, butylene glycol, propylene glycol, and sorbitol.
7. A fermented Ganoderma lucidum solution composition according to any of claims 1-6, wherein the polysaccharide compounds comprise beta-glucans and/or trehalose.
8. The ganoderma lucidum fermentation broth composition as claimed in claim 7, wherein the weight ratio of beta glucan to trehalose is (0.5-1): 1.
9. the ganoderma lucidum fermentation broth composition of claim 1, wherein the gelling agent comprises at least one of guar gum, sodium chloride, montmorillonite, PEG-150 distearate.
10. Use of the ganoderma lucidum fermentation broth composition according to any one of claims 1-9 in an anti-aging, moisturizing skin care product.
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