CN108095103A - A kind of Cordyceps militaris oral liquid and preparation method thereof - Google Patents
A kind of Cordyceps militaris oral liquid and preparation method thereof Download PDFInfo
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- CN108095103A CN108095103A CN201810068801.XA CN201810068801A CN108095103A CN 108095103 A CN108095103 A CN 108095103A CN 201810068801 A CN201810068801 A CN 201810068801A CN 108095103 A CN108095103 A CN 108095103A
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- cordyceps militaris
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- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 60
- 239000007788 liquid Substances 0.000 title claims abstract description 34
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 27
- 239000000706 filtrate Substances 0.000 claims abstract description 25
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000012141 concentrate Substances 0.000 claims abstract description 18
- 239000011780 sodium chloride Substances 0.000 claims abstract description 14
- 230000036039 immunity Effects 0.000 claims abstract description 8
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims description 18
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 16
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- 238000000855 fermentation Methods 0.000 claims description 8
- 230000004151 fermentation Effects 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 8
- 239000008103 glucose Substances 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 8
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 8
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 7
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 7
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 7
- 238000011534 incubation Methods 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 4
- 108010080698 Peptones Proteins 0.000 claims description 4
- 244000061456 Solanum tuberosum Species 0.000 claims description 4
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 4
- 229940041514 candida albicans extract Drugs 0.000 claims description 4
- 238000000386 microscopy Methods 0.000 claims description 4
- 238000009928 pasteurization Methods 0.000 claims description 4
- 235000019319 peptone Nutrition 0.000 claims description 4
- 230000008719 thickening Effects 0.000 claims description 4
- 239000012138 yeast extract Substances 0.000 claims description 4
- 239000003814 drug Substances 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 2
- 230000002708 enhancing effect Effects 0.000 claims description 2
- 235000013402 health food Nutrition 0.000 claims description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 1
- SEGLCEQVOFDUPX-UHFFFAOYSA-N di-(2-ethylhexyl)phosphoric acid Chemical compound CCCCC(CC)COP(O)(=O)OCC(CC)CCCC SEGLCEQVOFDUPX-UHFFFAOYSA-N 0.000 claims 1
- 229910052739 hydrogen Inorganic materials 0.000 claims 1
- 239000001257 hydrogen Substances 0.000 claims 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims 1
- 238000009630 liquid culture Methods 0.000 claims 1
- 239000011591 potassium Substances 0.000 claims 1
- 229910052700 potassium Inorganic materials 0.000 claims 1
- 239000004615 ingredient Substances 0.000 abstract description 4
- 235000019605 sweet taste sensations Nutrition 0.000 abstract description 2
- 238000006467 substitution reaction Methods 0.000 abstract 1
- 229960002743 glutamine Drugs 0.000 description 17
- 239000000047 product Substances 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- 241000382353 Pupa Species 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 description 3
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 description 3
- 230000035622 drinking Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 230000000474 nursing effect Effects 0.000 description 3
- 235000014347 soups Nutrition 0.000 description 3
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 210000002540 macrophage Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 210000001541 thymus gland Anatomy 0.000 description 2
- 241000235349 Ascomycota Species 0.000 description 1
- 241001480006 Clavicipitaceae Species 0.000 description 1
- 241000190633 Cordyceps Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 108010002352 Interleukin-1 Proteins 0.000 description 1
- 229930182816 L-glutamine Natural products 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 1
- 240000006698 Spigelia anthelmia Species 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003260 anti-sepsis Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 208000013116 chronic cough Diseases 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 210000000629 knee joint Anatomy 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 230000011278 mitosis Effects 0.000 description 1
- 206010029410 night sweats Diseases 0.000 description 1
- 230000036565 night sweats Effects 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000000242 pagocytic effect Effects 0.000 description 1
- 230000024241 parasitism Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000009923 sugaring Methods 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/40—Table salts; Dietetic salt substitutes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3571—Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of Cordyceps militaris oral liquids and preparation method thereof, which is mainly made of the component of following parts by weight：70~90 parts by weight of Cordyceps militaris filtrate concentrate, 1~5 parts by weight of glutamine, 0.2~0.5 parts by weight of sodium chloride.Prescription of the present invention is simple, definite ingredients, by Cordyceps militaris and the reasonable compatibility of glutamine, plays an important role of good strengthen immunity.And the present invention is suitble to a wide range of crowd to take with the sweet taste of fresh, salty, fragrant mouthfeel substitution conventional oral liquid.And prepare simple, suitable popularization and application.
Description
Technical field
The invention belongs to field of health care products, and in particular to a kind of Cordyceps militaris oral liquid that can enhance body immunity and its system
Preparation Method.
Background technology
Cordyceps militaris is Ascomycota, meat seat mesh, Clavicipitaceae, the type sepecies of Cordyceps.Scientific name is Cordyceps
Militaris also known as northern Cordyceps militaris, have area to be also known as the cordyceps sinensis that leaps up, the generally Cordceps militaris live body worm pupa culture calls pupa worm
Grass, the two are actually fungi of the same race.The worldwide distribution of Cordyceps militaris, natural resources quantity are seldom.Nineteen fifty, Germany scientist
Cunningham observes not perishable by the insect tissue of Cordyceps militaris parasitism, and then therefrom isolates a kind of antibacterial material,
3'-Deoxyadenosine is named as cordycepin.By stroma (i.e. careless part, also known as fructification) and sclerotium (i.e. the corpse part of insect)
Complex dimerous.The pupa of more infection lepidopterous insects.The traditional Chinese medical science thinks that cordycepic acid (mannitol) enters lung kidney two warp, both
Energy tonifying lung is cloudy, and energy kidney-replenishing, cures mainly kidney deficiency, and impotence and seminal emission, soreness of waist and knee joint, eak after being ill, chronic cough is weak, phthisical cough phlegm blood, from
Sweat night sweat etc. is a kind of unique Chinese medicine that can balance, adjust negative and positive simultaneously.
Glutamine, scientific name 2- amino -4- carbamyl butyric acid, be glutamic acid amide.L-Glutamine is protein
Coded amino acid in synthesis, mammal nonessential amino acid.Glutamine has important immunoregulation effect, it is leaching
Necessary to bar thin intracrine, multiplication and its function maintain.As the precursor and main energy sources of Nucleic acid, glutamine
It can promote lymphocyte, the mitosis of macrophage and differentiation and proliferation, increase generation and the phosphorus of cytokine TNF, IL-1 etc.
The mRNA synthesis of fat.The total number of lymphocytes of urgent patient, T lymphocytes can substantially be increased and follow by providing exogenous glutamine
The ratio of CD4/CD8 in ring enhances the immune function of body.
There are many health products of strengthen immunity currently on the market, but the guarantor of the strengthen immunity compounded with fungi and amino acid
Strong product do not have temporarily, and dosage form is mostly sweet taste for the health products of oral liquid, based on sugaring, honey or sweetener, monotonous taste.
The content of the invention
According to the above deficiency, it is contemplated that disclose that a kind of prescription is simple, definite ingredients, stability are good, taste is fresh fragrant
Cordyceps militaris oral liquid and preparation method thereof.
The purpose of the present invention is what is be accomplished by the following way：
A kind of Cordyceps militaris oral liquid, the oral liquid are mixed by the component of following parts by weight：
70~90 parts by weight of Cordyceps militaris filtrate concentrate, 1~5 parts by weight of glutamine, 0.2~0.5 parts by weight of sodium chloride.
It is preferred that above-mentioned oral liquid is mixed by the component of following parts by weight：
75~85 parts by weight of Cordyceps militaris filtrate concentrate, 2~4 parts by weight of glutamine, 0.2~0.5 parts by weight of sodium chloride.
Most preferably above-mentioned oral liquid is mixed by the component of following parts by weight：
80 parts by weight of Cordyceps militaris filtrate concentrate, 3 parts by weight of glutamine, 0.4 parts by weight of sodium chloride.
The preparation method of above-mentioned Cordyceps militaris oral liquid comprises the following steps：Cordyceps militaris filtrate concentrate is added into glutamine
And sodium chloride, stir and evenly mix, pasteurization to get.Above-mentioned Cordyceps militaris filtrate concentrate is to train Cordyceps militaris spawn deep layer liquid
The zymotic fluid obtained after supporting, isolated Cordyceps militaris filtrate, filtrate are concentrated into proportion as 1.1-1.4 by cryogenic vacuum, obtain
Cordyceps militaris filtrate concentrate.
The Cordyceps militaris spawn deep layer liquid incubation step is：Cordyceps militaris spawn activates, and 2~3 rings is taken to be inoculated in shaking flask
Culture medium is cultivated 4~5 days, 27~28 DEG C of cultivation temperature, and culture medium composition is：Potato 12%~15%, glucose 2%~
4%, peptone 0.5%~1%, potassium dihydrogen phosphate 0.1%~0.2%, magnesium sulfate 0.1%~0.2%, shake-flask seed is inoculated in
In fermentation tank, fermentation tank culture medium composition is：Glucose 2%~4%, yeast extract 1%~3%, potassium dihydrogen phosphate 0.1%
~0.2%, magnesium sulfate 0.1%~0.2%.27~28 DEG C of cultivation temperature, incubation time 5~6 days, zymotic fluid color are in the colour of loess
Color, sticky, microscopy has spore, without miscellaneous bacteria, and pH value is gone up to 3~4.0, and residual sugar drops to 1.0%, and reduced sugar drops to 0.5%.
The commercially available channel purchase of cordyceps militaris link bacterial strain, such as from the Chinese Academy of Sciences, Culture Collection obtains.
The cryogenic vacuum concentration condition is 70~80 DEG C of thickening temperature, and 0.07~0.08MPa of vacuum degree is concentrated into ratio
Weight is 1.1~1.2.
Above-mentioned Cordyceps militaris oral liquid can be applied in the health food or drug for preparing enhancing immunity of organisms.
Compared with the prior art, beneficial effects of the present invention：
1. prescription of the present invention is simple, definite ingredients, by Cordyceps militaris and the reasonable compatibility of glutamine, can be very good
To the effect of strengthen immunity；
2. the present invention retains the active ingredient in Cordyceps militaris filtrate well, without complicated extraction process, while greatly
It is big to reduce energy consumption, reduce alcohol consumption；
3. the present invention does not add any preservative, containing cordycepin ingredient in Cordyceps militaris, content 1.0-3.0mg/g, together
When can also play good antisepsis, the shelf-life of product is made to reach 24 months or more；
The sour-sweet mouthfeel of oral liquid 4. this product is broken the normal procedure, with the fresh of the fragrance of Cordyceps militaris filtrate uniqueness and glutamine
Taste combines, then is aided with sodium chloride, and sodium chloride can not only provide a small amount of saline taste, and can make the fragrance and glutamine of Cordyceps militaris
Delicate flavour it is more prominent and strong, make entire oral liquid that a kind of fragrant, fresh, salty mouthfeel be presented, just as the soup product for drinking nourishing,
It is easier to be esthetically acceptable to the consumers.
Specific embodiment：
It is further illustrated the present invention below by way of specific embodiment.But the detail of embodiment is only used for explaining this hair
It is bright, it should not be construed as limited overall technical solution.
Embodiment 1：
Cordyceps militaris spawn activates, and 2~3 rings is taken to be inoculated on Shake flask medium, cultivates 4 days, 27 DEG C of cultivation temperature, culture medium
It forms and is：Potato 15%, glucose 2.5%, peptone 0.8%, potassium dihydrogen phosphate 0.15%, magnesium sulfate 0.15%.Shaking flask
Seed is inoculated in fermentation tank, and fermentation tank culture medium composition is：Glucose 3%, yeast extract 2%, potassium dihydrogen phosphate
0.2%, magnesium sulfate 0.15%.27 DEG C of cultivation temperature, incubation time 5 days, for zymotic fluid color in khaki, sticky, microscopy has spore
Son, without miscellaneous bacteria, pH value is gone up to 4.0, and residual sugar drops to 1.0%, and reduced sugar, which drops to 0.5%, to carry out putting tank processing.
Above-mentioned zymotic fluid is taken into supernatant, dual-effect concentrator concentration, 70 DEG C of thickening temperature, vacuum degree through plate-frame filtering
0.08MPa is concentrated into 60 DEG C of proportions as 1.2 to get Cordyceps militaris filtrate concentrate.
80 parts by weight of Cordyceps militaris filtrate concentrate, 3 parts by weight of glutamine, 0.4 parts by weight of sodium chloride are taken, are stirred and evenly mixed,
Pasteurization, 10min are filled into bottle to get Cordyceps militaris oral liquid.
The oral liquid mouthfeel is fragrant, fresh, just as the soup product for drinking nourishing, 24 months shelf-lifves of product.
Embodiment 2：
Cordyceps militaris spawn Conventional activation takes 2~3 rings to be inoculated on Shake flask medium, cultivates 4 days, 28 DEG C of cultivation temperature, training
Supporting base composition is：Potato 13%, glucose 3%, peptone 0.6%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.2%.Shaking flask
Seed is inoculated in fermentation tank, and fermentation tank culture medium composition is：Glucose 4%, yeast extract 3%, potassium dihydrogen phosphate
0.1%, magnesium sulfate 0.1%.28 DEG C of cultivation temperature, incubation time 6 days, for zymotic fluid color in khaki, sticky, microscopy has spore
Son, without miscellaneous bacteria, pH value is gone up to 4.0, and residual sugar drops to 1.0%, and reduced sugar, which drops to 0.5%, to carry out putting tank processing.
Above-mentioned zymotic fluid is taken into supernatant, dual-effect concentrator concentration, 80 DEG C of thickening temperature, vacuum degree through plate-frame filtering
0.07MPa is concentrated into proportion as 1.15 to get Cordyceps militaris filtrate concentrate.
75 parts by weight of Cordyceps militaris filtrate concentrate, 4 parts by weight of glutamine, 0.2 parts by weight of sodium chloride are taken, are stirred and evenly mixed,
Pasteurization, 10min are filled into bottle to get Cordyceps militaris oral liquid.
The oral liquid mouthfeel is fragrant, fresh, micro- salty, just as the soup product for drinking nourishing, 24 months shelf-lifves of product.
The present invention is further described below by specific test example：
This experiment is divided into 4 groups, and every group of 20 mouse, wherein blank group are basic forage feed, are raised based on 1 group of experiment
Material+embodiment 1 is formulated (2mL) nursing, and 2 groups of experiment is basic feed+Cordyceps militaris filtrate concentrate (according to 1 method institute of embodiment
The Cordyceps militaris filtrate concentrate preparation process stated obtains) (5mL) nursing, 3 groups of experiment is basic feed+glutamine aqueous solution
(3.6%) (2mL) is fed, and nursing time detects its thymus index, lymphopoiesis situation and macrophage after being 20 days, 20 days
Cytophagic index, specific data are shown in Table 1.
Table 1
| Group | Size of animal (only) | Thymus index | Optical density difference | Phagocytic index |
| Blank group | 20 | 14.34±1.23 | 0.0528±0.0176 | 21.67±6.53 |
| Test 1 group | 20 | 30.56±3.28 | 0.3758±0.0352 | 33.57±7.22 |
| Test 2 groups | 20 | 20.67±2.84 | 0.1539±0.0278 | 26.59±5.49 |
| Test 3 groups | 20 | 19.21±1.97 | 0.1481±0.0304 | 24.37±6.28 |
It follows that the present invention plays an important role of to increase immunity of organisms, and there is collaboration to increase for glutamine and Cordyceps militaris
Effect acts on.
Claims (8)
1. a kind of Cordyceps militaris oral liquid, it is characterised in that the oral liquid is mixed by the component of following parts by weight：
70~90 parts by weight of Cordyceps militaris filtrate concentrate, 1~5 parts by weight of glutamine, 0.2~0.5 parts by weight of sodium chloride.
2. Cordyceps militaris oral liquid according to claim 1, it is characterised in that the oral liquid is by the component of following parts by weight
It is mixed：
75~85 parts by weight of Cordyceps militaris filtrate concentrate, 2~4 parts by weight of glutamine, 0.2~0.5 parts by weight of sodium chloride.
3. Cordyceps militaris oral liquid according to claim 1, it is characterised in that the oral liquid is by the component of following parts by weight
It is mixed：
80 parts by weight of Cordyceps militaris filtrate concentrate, 3 parts by weight of glutamine, 0.4 parts by weight of sodium chloride.
4. the preparation method of the Cordyceps militaris oral liquid described in a kind of claim 1,2 or 3, it is characterised in that this method includes following
Step：Cordyceps militaris filtrate concentrate is added into glutamine and sodium chloride, is stirred and evenly mixed, pasteurization to get.
5. the preparation method of Cordyceps militaris oral liquid according to claim 4, it is characterised in that the Cordyceps militaris filtrate is dense
Contracting liquid is the zymotic fluid that will be obtained after Cordyceps militaris spawn deep layer liquid culture, and isolated Cordyceps militaris filtrate, filtrate is by low temperature
Proportion is concentrated in vacuo to as 1.1-1.4, obtains Cordyceps militaris filtrate concentrate.
6. the preparation method of Cordyceps militaris oral liquid according to claim 5, it is characterised in that the Cordyceps militaris spawn is deep
Layer liquid incubation step be：Cordyceps militaris spawn activates, and 2~3 rings is taken to be inoculated in Shake flask medium, cultivates 4~5 days, cultivation temperature
27~28 DEG C, culture medium, which forms, is：Potato 12%~15%, glucose 2%~4%, peptone 0.5%~1%, di(2-ethylhexyl)phosphate
Hydrogen potassium 0.1%~0.2%, magnesium sulfate 0.1%~0.2%, shake-flask seed is inoculated in fermentation tank, fermentation tank culture medium composition
For：Glucose 2%~4%, yeast extract 1%~3%, potassium dihydrogen phosphate 0.1%~0.2%, magnesium sulfate 0.1%~
0.2%.27~28 DEG C of cultivation temperature, incubation time 5~6 days, for zymotic fluid color in khaki, sticky, microscopy has spore, without miscellaneous
Bacterium, pH value are gone up to 3~4.0, and residual sugar drops to 1.0%, and reduced sugar drops to 0.5%.
7. the preparation method of Cordyceps militaris oral liquid according to claim 5, it is characterised in that the cryogenic vacuum concentration
Condition is 70~80 DEG C, 0.07~0.08MPa of vacuum degree of thickening temperature, is concentrated into proportion as 1.1~1.2.
8. the Cordyceps militaris oral liquid described in claim 1,2 or 3 is in the health food or drug for preparing enhancing immunity of organisms
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