CN115364132A - Preparation method of angelica sinensis extract - Google Patents
Preparation method of angelica sinensis extract Download PDFInfo
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- CN115364132A CN115364132A CN202211207735.2A CN202211207735A CN115364132A CN 115364132 A CN115364132 A CN 115364132A CN 202211207735 A CN202211207735 A CN 202211207735A CN 115364132 A CN115364132 A CN 115364132A
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- 241000382455 Angelica sinensis Species 0.000 title claims abstract description 36
- 239000000284 extract Substances 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 241000125175 Angelica Species 0.000 claims abstract description 69
- 235000001287 Guettarda speciosa Nutrition 0.000 claims abstract description 69
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 65
- 229960000583 acetic acid Drugs 0.000 claims abstract description 29
- 239000012362 glacial acetic acid Substances 0.000 claims abstract description 22
- 238000002137 ultrasound extraction Methods 0.000 claims abstract description 20
- 238000010438 heat treatment Methods 0.000 claims abstract description 19
- 238000002156 mixing Methods 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000000706 filtrate Substances 0.000 claims abstract description 13
- 238000001035 drying Methods 0.000 claims abstract description 11
- 238000000605 extraction Methods 0.000 claims abstract description 10
- 210000004911 serous fluid Anatomy 0.000 claims abstract description 10
- 238000001914 filtration Methods 0.000 claims abstract description 9
- 239000002904 solvent Substances 0.000 claims abstract description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 24
- 239000002736 nonionic surfactant Substances 0.000 claims description 10
- 108090000790 Enzymes Proteins 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 9
- 229940088598 enzyme Drugs 0.000 claims description 9
- 108010059892 Cellulase Proteins 0.000 claims description 8
- 229940106157 cellulase Drugs 0.000 claims description 8
- 108010059820 Polygalacturonase Proteins 0.000 claims description 6
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 6
- 229920000053 polysorbate 80 Polymers 0.000 claims description 6
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 4
- 229920001030 Polyethylene Glycol 4000 Polymers 0.000 claims description 4
- 239000004480 active ingredient Substances 0.000 abstract description 15
- 210000004027 cell Anatomy 0.000 abstract description 9
- 210000002421 cell wall Anatomy 0.000 abstract description 7
- 239000002994 raw material Substances 0.000 abstract description 5
- 238000009792 diffusion process Methods 0.000 abstract description 3
- 230000000149 penetrating effect Effects 0.000 abstract description 3
- 230000035699 permeability Effects 0.000 abstract description 3
- 239000003814 drug Substances 0.000 abstract description 2
- 239000002002 slurry Substances 0.000 description 7
- KSEBMYQBYZTDHS-HWKANZROSA-M (E)-Ferulic acid Natural products COC1=CC(\C=C\C([O-])=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-M 0.000 description 6
- IQVQXVFMNOFTMU-FLIBITNWSA-N (Z)-ligustilide Chemical compound C1CC=CC2=C1C(=C/CCC)/OC2=O IQVQXVFMNOFTMU-FLIBITNWSA-N 0.000 description 6
- IQVQXVFMNOFTMU-DHZHZOJOSA-N Z-ligustilide Natural products C1CC=CC2=C1C(=C/CCC)\OC2=O IQVQXVFMNOFTMU-DHZHZOJOSA-N 0.000 description 6
- KSEBMYQBYZTDHS-HWKANZROSA-N ferulic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-N 0.000 description 6
- 229940114124 ferulic acid Drugs 0.000 description 6
- KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 description 6
- 235000001785 ferulic acid Nutrition 0.000 description 6
- QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 description 6
- 238000004537 pulping Methods 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 4
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 239000000341 volatile oil Substances 0.000 description 3
- 201000000736 Amenorrhea Diseases 0.000 description 2
- 206010001928 Amenorrhoea Diseases 0.000 description 2
- 206010007247 Carbuncle Diseases 0.000 description 2
- 206010010774 Constipation Diseases 0.000 description 2
- 208000005171 Dysmenorrhea Diseases 0.000 description 2
- 206010013935 Dysmenorrhoea Diseases 0.000 description 2
- 231100000540 amenorrhea Toxicity 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 230000005906 menstruation Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 2
- 230000008736 traumatic injury Effects 0.000 description 2
- 208000004998 Abdominal Pain Diseases 0.000 description 1
- 241000208173 Apiaceae Species 0.000 description 1
- 208000006820 Arthralgia Diseases 0.000 description 1
- 208000037093 Menstruation Disturbances Diseases 0.000 description 1
- 206010027339 Menstruation irregular Diseases 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 206010033557 Palpitations Diseases 0.000 description 1
- 208000025747 Rheumatic disease Diseases 0.000 description 1
- 208000012886 Vertigo Diseases 0.000 description 1
- PGLIMMMHQDNVRS-YZQQHVNFSA-N [(e)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enyl] (e)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoate Chemical compound C1=C(O)C(OC)=CC(\C=C\COC(=O)\C=C\C=2C=C(OC)C(O)=CC=2)=C1 PGLIMMMHQDNVRS-YZQQHVNFSA-N 0.000 description 1
- 239000009188 angelicae sinensis extract Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- PGLIMMMHQDNVRS-UHFFFAOYSA-N coniferyl ferulate Natural products C1=C(O)C(OC)=CC(C=CCOC(=O)C=CC=2C=C(OC)C(O)=CC=2)=C1 PGLIMMMHQDNVRS-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000000552 rheumatic effect Effects 0.000 description 1
- 239000001384 succinic acid Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 231100000889 vertigo Toxicity 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a preparation method of an angelica sinensis extract, and relates to the technical field of traditional Chinese medicine extraction. The preparation method of the angelica sinensis extract comprises the following steps: mixing fresh angelica with water, and performing tissue disruption to obtain angelica serous fluid; mixing the angelica sinensis serous fluid with glacial acetic acid, then carrying out steam heating, enzymolysis, ultrasonic extraction and filtration, and concentrating and drying the filtrate to obtain the angelica sinensis extract. The invention takes fresh angelica as raw material, omits the step of drying angelica, reduces the loss of effective components and saves time; glacial acetic acid is utilized to destroy the cell wall of the angelica as much as possible, the permeability inside and outside the cell is increased, and then the active ingredients inside the angelica cell reach the surface as much as possible by means of the penetrating power of steam heating; the enzymolysis can make the cell wall of the angelica more fragile and accelerate the diffusion of the active ingredients of the angelica; ultrasonic extraction can promote most of the active ingredients in the angelica cells to be dissolved in the solvent, so that the active ingredients of the angelica can be extracted more fully.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicine extraction, and particularly relates to a preparation method of an angelica sinensis extract.
Background
Angelica sinensis, a root of Angelica sinensis (oliv.) Diels, belonging to the family Umbelliferae, has the effects of tonifying blood, promoting blood circulation, regulating menstruation, relieving pain, and loosening bowel to relieve constipation. It is commonly indicated for blood deficiency and sallow complexion, vertigo and palpitation, irregular menstruation, amenorrhea and dysmenorrhea, deficiency cold and abdominal pain, rheumatic arthralgia, traumatic injury, carbuncle, deep-rooted carbuncle, pyocutaneous disease, constipation due to intestinal dryness. The wine angelica sinensis can activate blood and promote menstruation. Can be used for treating amenorrhea, dysmenorrhea, rheumatalgia, and traumatic injury.
The chemical components of the angelica are mainly divided into volatile oil and water-soluble components. The radix Angelicae sinensis volatile oil contains ligustilide as main ingredient, and the water soluble components include ferulic acid, coniferyl ferulate, and succinic acid. Due to the difference in chemical structures between the volatile oil and the water-soluble components of Angelica sinensis, separate extraction is required for extracting the active components of Angelica sinensis. This greatly increases the cost of studying the pharmacological activity of the more comprehensive active ingredient of angelica. Therefore, it is highly desirable to invent a preparation method for comprehensively extracting the active ingredients from angelica.
Disclosure of Invention
The invention aims to provide a preparation method of an angelica sinensis extract, which can obviously improve the content of effective components in angelica sinensis.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a preparation method of an angelica sinensis extract, which comprises the following steps: mixing fresh angelica with water, and performing tissue disruption to obtain angelica serous fluid; mixing the angelica sinensis serous fluid with glacial acetic acid, then carrying out steam heating, enzymolysis, ultrasonic extraction and filtration, and concentrating and drying the filtrate to obtain the angelica sinensis extract.
Preferably, the weight volume ratio of the fresh angelica to the water is 1-4g/mL.
Preferably, the weight volume ratio of the fresh angelica to the glacial acetic acid is 1-3.
Preferably, the steam heating temperature is 100-120 ℃ and the time is 5-30min.
Preferably, the enzyme for enzymolysis comprises one or two of cellulase and pectinase; the addition amount of the enzyme is 1-3% of the weight of the fresh angelica.
Preferably, the enzymolysis temperature is 45-55 ℃ and the time is 20-30min.
Preferably, a nonionic surfactant is added in the enzymolysis process; the nonionic surfactant comprises tween 80 or PEG-4000.
Preferably, the weight ratio of the nonionic surfactant to the enzyme is 1 (5-10).
Preferably, the solvent for ultrasonic extraction is an ethanol solution; the mass volume fraction of the ethanol solution is 70-95%; the volume of the ethanol solution is 3-6 times of that of the glacial acetic acid.
Preferably, the power of the ultrasonic extraction is 25-40kHz, the temperature is 30-50 ℃, the extraction times are 1-4 times, and each time of extraction is 10-20min.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a preparation method of an angelica sinensis extract, which comprises the following steps: mixing fresh angelica with water, and performing tissue disruption to obtain angelica serous fluid; mixing the angelica sinensis serous fluid with glacial acetic acid, then carrying out steam heating, enzymolysis, ultrasonic extraction and filtration, and concentrating and drying the filtrate to obtain the angelica sinensis extract. The invention takes fresh angelica as raw material, omits the step of drying angelica, reduces the loss of effective components and saves time; glacial acetic acid is utilized to destroy the cell wall of the angelica as much as possible, the permeability inside and outside the cell is increased, and then the active ingredients inside the angelica cell reach the surface as much as possible by means of the penetrating power of steam heating; the enzymolysis can make the cell wall of the angelica more fragile and accelerate the diffusion of the active ingredients of the angelica; ultrasonic extraction can promote most of the active ingredients in the angelica cells to be dissolved in the solvent, so that the active ingredients of the angelica can be extracted more fully.
Detailed Description
The invention provides a preparation method of an angelica sinensis extract, which comprises the following steps: mixing fresh angelica with water, and performing tissue disruption to obtain angelica serous fluid; mixing the angelica sinensis serous fluid with glacial acetic acid, then carrying out steam heating, enzymolysis, ultrasonic extraction and filtration, and concentrating and drying the filtrate to obtain the angelica sinensis extract.
In the present invention, the fresh angelica is preferably a 2-year-old angelica root; the fresh angelica is collected in Gansu Min county; the weight volume ratio of the fresh angelica to the water is preferably 1-4g/mL, more preferably 1. The invention adopts the fresh angelica with high water content as the raw material, and water is added in the step, so that the pulping effect of the fresh angelica can be better ensured; in addition, the invention takes fresh angelica as raw material, omits the step of drying the angelica and reduces the loss of effective components.
In the invention, the weight volume ratio of the fresh angelica to the glacial acetic acid is 1-3, more preferably 1g/mL; the steam heating temperature is preferably 100-120 deg.C, more preferably 110 deg.C, and time is preferably 5-30min, more preferably 20min. The invention utilizes glacial acetic acid to destroy the cell wall of the angelica as much as possible, increases the permeability inside and outside the cell, and leads the active ingredients inside the angelica cell to reach the surface as much as possible by means of the penetrating power of steam heating, thereby improving the extraction of the active ingredients of the angelica.
In the present invention, the enzyme for enzymolysis preferably comprises one or two of cellulase and pectinase, more preferably cellulase and pectinase, wherein the weight ratio of cellulase to pectinase is 2; the addition amount of the enzyme is preferably 1-3% of the weight of fresh angelica, and more preferably 2%; the temperature of the enzymolysis is preferably 45-55 ℃, more preferably 50 ℃, and the time is preferably 20-30min, more preferably 25min; preferably, a nonionic surfactant is added in the enzymolysis process; the non-ionic surfactant preferably comprises tween 80 or PEG-4000, more preferably tween 80; the weight ratio of the nonionic surfactant to the enzyme is preferably 1 (5-10), more preferably 1. The enzymolysis step can carry out enzymolysis on the cell wall of the angelica, so that the effective components reaching the cell wall can accelerate the diffusion of the active components of the angelica; the nonionic surfactant in the enzymolysis step can obviously improve the enzymolysis speed and save time.
In the present invention, the solvent for the ultrasonic extraction is preferably an ethanol solution; the mass volume fraction of the ethanol solution is preferably 70-95%, and more preferably 80%; the volume of the ethanol solution is preferably 3-6 times, and more preferably 4 times of that of glacial acetic acid; the power of the ultrasonic extraction is preferably 25-40kHz, more preferably 30kHz, the temperature is preferably 30-50 ℃, more preferably 40 ℃, the extraction times are preferably 1-4 times, more preferably 2 times, and each time is preferably 10-20min, more preferably 15min. The ultrasonic extraction of the invention can promote most active ingredients in the angelica cells to be dissolved in the solvent, so that the extraction of the active ingredients of the angelica is more sufficient.
In the present invention, unless otherwise specified, all the raw material components are commercially available products well known to those skilled in the art.
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
(1) Pulping: adding 2 times of water solution into 1kg of 2-year-old fresh angelica for tissue disruption to obtain angelica slurry;
(2) Heating glacial acetic acid by steam: adding 500mL glacial acetic acid into the radix Angelicae sinensis slurry, and steam heating at 110 deg.C for 20min to obtain radix Angelicae sinensis acetic acid vapor;
(3) Enzymolysis: cooling the angelica acetic acid vapor to room temperature, mixing with 20g of cellulase, 10g of pectinase and 3.75g of Tween 80, and performing enzymolysis at 50 ℃ for 25min to obtain an angelica zymolyte;
(4) Ultrasonic extraction: extracting the radix Angelicae sinensis zymolyte with 2000mL 80% ethanol solution at 30kHz and 40 deg.C for 15min for 2 times, filtering, mixing filtrates, filtering, and mixing filtrates to obtain radix Angelicae sinensis ultrasonic filtrate;
(5) Concentrating and drying to obtain the angelica extract.
Example 2
(1) Pulping: adding 3 times of water solution into 1kg of 2-year-old fresh angelica for tissue disruption to obtain angelica slurry;
(2) Heating glacial acetic acid by steam: adding 1000mL glacial acetic acid into the radix Angelicae sinensis slurry, and steam heating at 100 deg.C for 30min to obtain radix Angelicae sinensis acetic acid vapor;
(3) Enzymolysis: cooling the angelica acetic acid vapor to room temperature, mixing with 30g of cellulase and 6g of PEG-4000, and performing enzymolysis at 45 ℃ for 30min to obtain an angelica zymolyte;
(4) Ultrasonic extraction: extracting radix Angelicae sinensis zymolyte with 3000mL 95% ethanol solution at 25kHz and 50 deg.C for 10min for 4 times, filtering, and mixing filtrates to obtain radix Angelicae sinensis ultrasonic filtrate;
(5) Concentrating, and drying to obtain radix Angelicae sinensis extract.
Example 3
(1) Pulping: adding 1kg of water solution in an amount which is 1 time that of the fresh angelica for 2 years into the fresh angelica for tissue disruption to obtain angelica slurry;
(2) Heating glacial acetic acid by steam: adding 250mL glacial acetic acid into the radix Angelicae sinensis slurry, and steam heating at 120 deg.C for 5min to obtain radix Angelicae sinensis acetic acid vapor;
(3) Enzymolysis: cooling the acetic acid vapor of radix Angelicae sinensis to room temperature, mixing with 10g cellulase and 1g tween 80, and performing enzymolysis at 55 deg.C for 20min to obtain radix Angelicae sinensis zymolyte;
(4) Ultrasonic extraction: extracting radix Angelicae sinensis zymolyte with 1500mL 70% ethanol solution at 40kHz and 30 deg.C for 20min, and filtering to obtain radix Angelicae sinensis ultrasonic filtrate;
(5) And concentrating and drying the angelica ultrasonic filtrate to obtain the angelica extract.
Comparative example 1
The specific implementation manner is the same as that of example 1, except that step (2) is not provided, and the angelica slurry of step (1) is directly subjected to an enzymolysis step.
Comparative example 2
The specific implementation manner is the same as that of example 1, except that the angelica acetic acid vapor obtained in step (2) is directly subjected to ultrasonic extraction without the step (3).
Comparative example 3
The specific implementation manner is the same as that of example 1, except that the step (4) is omitted, and the angelica sinensis zymolyte obtained in the step (3) is filtered, and then the filtrate is concentrated and dried to obtain the angelica sinensis extract.
Experimental example 1
HPLC (compare contents of ferulic acid, ligustilide and total polysaccharide in Angelica sinensis of different origins, pages 37-38) is used to simultaneously detect the contents of ligustilide and ferulic acid in the Angelica sinensis extracts obtained in examples 1-3 and comparative examples 1-3, and specific results are shown in Table 1.
TABLE 1 content of ligustilide and ferulic acid in different Angelica sinensis extracts
As can be seen from the data in table 1, the content of ligustilide and ferulic acid in the angelica sinensis extract can be significantly increased by using the angelica sinensis extract obtained by the preparation method of the present invention, compared to the angelica sinensis extract without the steps of glacial acetic acid steam heating, enzymolysis, and ultrasonic extraction.
The invention carries out the steps of glacial acetic acid steam heating, enzymolysis and ultrasonic extraction in sequence after pulping the fresh angelica, and the steps are closely connected, thereby having synergistic effect, leading the active ingredients of the angelica to be more fully extracted and improving the content of ligustilide and ferulic acid in the angelica extract.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. The preparation method of the angelica sinensis extract is characterized by comprising the following steps:
mixing fresh angelica with water, and performing tissue disruption to obtain angelica serous fluid;
mixing the angelica sinensis serous fluid with glacial acetic acid, then carrying out steam heating, enzymolysis, ultrasonic extraction and filtration, and concentrating and drying the filtrate to obtain the angelica sinensis extract.
2. The preparation method according to claim 1, wherein the weight volume ratio of the fresh angelica sinensis to the water is 1-4g/mL.
3. The preparation method according to claim 1, wherein the weight-to-volume ratio of the fresh angelica sinensis to the glacial acetic acid is 1-3.
4. The method of claim 1, wherein the steam heating is performed at a temperature of 100 to 120 ℃ for 5 to 30min.
5. The preparation method according to claim 1, wherein the enzyme for enzymolysis comprises one or two of cellulase and pectinase; the addition amount of the enzyme is 1-3% of the weight of the fresh angelica.
6. The preparation method of claim 5, wherein the enzymolysis temperature is 45-55 deg.C, and the time is 20-30min.
7. The preparation method according to claim 5, wherein a nonionic surfactant is added during the enzymolysis; the nonionic surfactant comprises tween 80 or PEG-4000.
8. The method according to claim 5 or 7, wherein the weight ratio of the nonionic surfactant to the enzyme is 1 (5-10).
9. The method of claim 1, wherein the solvent for ultrasonic extraction is an ethanol solution; the mass volume fraction of the ethanol solution is 70-95%; the volume of the ethanol solution is 3-6 times of that of the glacial acetic acid.
10. The method of claim 9, wherein the power of the ultrasonic extraction is 25-40kHz, the temperature is 30-50 ℃, the number of extractions is 1-4, and each extraction is 10-20min.
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Citations (2)
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| CN102381962A (en) * | 2011-10-19 | 2012-03-21 | 兰州理工大学 | Extraction method of effective components of Chinese angelica |
| CN109867598A (en) * | 2017-12-01 | 2019-06-11 | 北京国康本草物种生物科学技术研究院有限公司 | Radix Angelicae Sinensis extraction process of effective component and its extract |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102381962A (en) * | 2011-10-19 | 2012-03-21 | 兰州理工大学 | Extraction method of effective components of Chinese angelica |
| CN109867598A (en) * | 2017-12-01 | 2019-06-11 | 北京国康本草物种生物科学技术研究院有限公司 | Radix Angelicae Sinensis extraction process of effective component and its extract |
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