CN115363205A - Composition with immunity enhancing and anti-inflammatory functions and preparation method thereof - Google Patents

Composition with immunity enhancing and anti-inflammatory functions and preparation method thereof Download PDF

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CN115363205A
CN115363205A CN202210952876.0A CN202210952876A CN115363205A CN 115363205 A CN115363205 A CN 115363205A CN 202210952876 A CN202210952876 A CN 202210952876A CN 115363205 A CN115363205 A CN 115363205A
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powder
parts
aloe
composition
royal jelly
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喻勤
余宗盛
汤酿
谢岚
韦建明
袁方
陆少敏
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Perfect Guangdong Commodity Co Ltd
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Perfect Guangdong Commodity Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
    • A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention discloses a composition with functions of enhancing immunity and resisting inflammation and a preparation method thereof, and relates to the technical field of compositions. The invention provides a composition with immunity enhancing and anti-inflammatory functions, which comprises the following components: aloe powder, lyophilized royal jelly powder, wall-broken rape pollen and turmeric; wherein, the weight ratio of the aloe powder, the royal jelly freeze-dried powder, the wall-broken rape pollen and the turmeric is as follows: aloe powder: lyophilized royal jelly powder: wall breaking of rape pollen: turmeric = (10-30): (20-40): (20-40): (10-30). The composition with the immunity enhancing and anti-inflammatory functions is safe and suitable for long-term use, raw materials of the composition with the immunity enhancing and anti-inflammatory functions are mutually cooperated, and the prepared product has good immunity enhancing and anti-inflammatory effects.

Description

Composition with immunity enhancing and anti-inflammatory functions and preparation method thereof
Technical Field
The invention relates to the technical field of compositions, in particular to a composition with functions of enhancing immunity and resisting inflammation and a preparation method thereof.
Background
Immunity is the body's own defense mechanism, and is the body's ability to recognize and destroy any foreign body (virus, bacteria, etc.) that invades from the outside, to treat aged, damaged, dead, denatured self cells, and to recognize and treat mutant cells and virus-infected cells in the body. Inflammation refers to a series of defense reaction processes of recognition, elimination and repair of infectious or non-infectious injury factors generated by living tissues with a vascular system, and acute inflammation is mainly manifested by red, swelling, heat, pain and dysfunction. According to the cause of inflammation, the inflammation is divided into infectious inflammation and aseptic inflammation.
Aloe is a perennial fleshy herbaceous plant of the genus Aloe of the family Liliaceae, and is in various kinds, about 500 kinds, and can be medicinal aloe, edible aloe and ornamental aloe according to its use. Aloe is rich in various bioactive substances, including anthraquinone compounds, saccharide compounds, amino acids, organic acids, vitamins, steroid compounds, enzyme compounds, inorganic substances, etc., wherein Aloe polysaccharide is the main bioactive component of Aloe gel. The aloe species which can be used as the raw material of health food are aloe vera and aloe ferox according to relevant regulations of health food in China. Royal jelly is secretion of pharyngeal gland of young worker bee (nanny bee) of larva cultured in honeycomb, and is slurry substance for feeding bee larva and queen bee. The royal jelly contains glucose, lipid, protein, mineral, vitamins, aspartic acid, phosphorus compounds, sterol, gel, nucleic acid, trace components, acetylcholine, etc. The royal jelly generally contains 65-68% of water, 11-14% of protein, 15-17% of carbohydrate, 6% of lipid and 3% of other substances. The lyophilized royal jelly powder is prepared from fresh royal jelly by filtering, lyophilizing, pulverizing, etc., and can well retain the nutritional components therein by lyophilization. The wall-broken rape pollen is prepared by drying rape pollen, pulverizing, and breaking cell wall with air flow. Rape pollen is one kind of common bee pollen, and bee pollen is oblate particle prepared by collecting pollen from plant pistil, adding nectar and saliva, and mixing. Pollen not only contains nutrients such as protein, fat, sugar, trace elements and various vitamins which are usually needed by human body, but also contains a plurality of bioactive substances such as flavone, carotenoid, phospholipid, polyunsaturated fatty acid and the like, so the pollen is known as a 'micro nutrient bank'. The turmeric is prepared by extracting and refining rhizome of turmeric of Zingiberaceae with solvent, and the turmeric has bitter and pungent taste, warm property, liver and spleen channels, good pharmacological activity, oxidation resistance, various pathogenic microorganisms resistance, rheumatism resistance, tumor resistance, and immune system, cardiovascular system, digestive system and nervous system function improvement.
At present, most products on the market mainly have single effects of enhancing immunity or resisting inflammation, and few products have the effects of enhancing immunity and resisting inflammation.
Disclosure of Invention
Based on the above, the invention aims to overcome the defects of the prior art and provide a composition with the functions of enhancing immunity and resisting inflammation and a preparation method thereof. The composition with the immunity enhancing and anti-inflammatory functions is safe and suitable for long-term use, raw materials of the composition with the immunity enhancing and anti-inflammatory functions are mutually cooperated, and the prepared product has good immunity enhancing and anti-inflammatory effects.
In order to realize the purpose, the technical scheme adopted by the invention is as follows: a composition with immunity enhancing and anti-inflammatory functions comprises the following components: aloe powder, lyophilized royal jelly powder, wall-broken rape pollen and turmeric; wherein, the weight ratio of the aloe powder, the royal jelly freeze-dried powder, the wall-broken rape pollen and the turmeric is as follows: aloe powder: lyophilized royal jelly powder: wall breaking of rape pollen: turmeric = (10-30): (20-40): (20-40): (10-30).
The invention provides a composition comprising aloe powder, royal jelly freeze-dried powder, wall-broken rape pollen and turmeric, wherein aloe polysaccharide in the aloe powder, flavone in the wall-broken rape pollen, protein in the royal jelly freeze-dried powder and polyphenol substances in the turmeric in the composition exert multi-aspect regulating effects on an organism immune system by acting on immune organs (thymus and spleen), activating immune cells (lymphocytes, mononuclear phagocytes and leukocytes), regulating cytokine release, promoting antibody generation and the like, so that the composition plays a synergistic role and improves the whole immunity of the organism. Meanwhile, under the condition of improving the whole immunity of the organism, the composition plays a synergistic role, reduces the occurrence of oxidative stress reaction, inhibits inflammatory mediators and transcription factors, and plays a better anti-inflammatory role, thereby reducing the occurrence of chronic diseases.
The aloe powder, the royal jelly freeze-dried powder, the wall-broken rape pollen and the turmeric used in the invention can be purchased directly or prepared by adopting corresponding preparation methods.
Preferably, the composition with the functions of enhancing immunity and resisting inflammation comprises the following components in percentage by weight: aloe powder: lyophilized royal jelly powder: breaking cell walls of rape pollen: turmeric = (10-23): (27-40): (27-40): (10-23).
The inventor finds that when the proportion of the aloe powder, the royal jelly freeze-dried powder, the wall-broken rape pollen and the turmeric in the composition is changed, the effects of immunity and inflammation resistance are directly influenced. The effect of the gradual increase or decrease of the content of the single raw material on the effect of the composition is not linear, and the composition has better immunity enhancing and anti-inflammatory effects when the proportion is increased.
Preferably, the composition with the functions of enhancing immunity and resisting inflammation comprises the following components in parts by weight: aloe powder: lyophilized royal jelly powder: breaking cell walls of rape pollen: turmeric =15:35:35:15.
the inventor finds that when the proportion of the aloe powder, the royal jelly freeze-dried powder, the wall-broken rape pollen and the turmeric in the composition is 15:35:35:15, the composition has optimal effects of enhancing immunity and resisting inflammation.
Preferably, the aloe powder is aloe gel powder, and the mass percentage content of polysaccharide in the aloe gel powder is more than or equal to 80000mg/kg; in the aloe gel powder, the mass percentage of O-acetyl is more than or equal to 75000mg/kg calculated by acetylcholine chloride. The aloe gel powder selected by the invention meets the requirements of QB/T2489-2018 aloe products for food raw materials.
Preferably, the turmeric comprises curcumin, demethoxycurcumin and bisdemethoxycurcumin, the turmeric selected by the invention meets the requirement of GB 1886.60-2015 national standard for food safety-food additive-turmeric, and the turmeric has the following structural formula:
Figure BDA0003786762230000031
wherein R in curcumin 1 ＝R 2 ＝OCH 3 R in demethoxycurcumin 1 ＝OCH 3 ，R 2 = H, R in bisdemethoxycurcumin 1 ＝R 2 ＝H。
Preferably, the royal jelly freeze-dried powder selected by the invention meets the requirements of GB/T21532-2008 royal jelly freeze-dried powder; the rape pollen selected by the invention meets the requirements of GB 31636-2016 pollen which is a national standard for food safety.
In addition, another object of the present invention is to provide a method for preparing the composition having immunity-enhancing and anti-inflammatory functions, comprising the steps of: mixing Aloe powder, lac Regis Apis lyophilized powder, cell wall broken rape pollen and Curcuma rhizome uniformly to obtain the composition with immunity enhancing and antiinflammatory functions.
Still another object of the present invention is to provide the use of said composition in the preparation of an article or food having immune enhancing and anti-inflammatory functions.
Preferably, the article of manufacture comprises a composition having immune enhancing and anti-inflammatory functions.
Preferably, the product further comprises auxiliary materials, wherein the auxiliary materials are at least one of dietary fibers, compound nutrients, amino acids, fillers, acidity regulators, sweeteners, fruit and vegetable powder, essence and flow aids.
Preferably, the article is at least one of the following (a) to (i):
(a) The dietary fiber is at least one of fructo-oligosaccharide, xylo-oligosaccharide, galacto-oligosaccharide, isomalto-oligosaccharide, inulin and resistant dextrin;
(b) The compound nutrient is a mixture of nutrient and mineral; the nutrient is at least one of vitamin C, vitamin E, vitamin A and vitamin D; the mineral substance is at least one of calcium salt, magnesium salt, zinc salt, chromium salt and iron salt;
(c) The amino acid is at least one of taurine, alanine, arginine, leucine, isoleucine and valine;
(d) The filler is at least one of erythritol, fructose, maltitol, sorbitol, isomalt, xylitol and lactose;
(e) The sour agent is at least one of citric acid, malic acid, lactic acid, fumaric acid and tartaric acid;
(f) The sweetener is at least one of stevioside, mogroside, sucralose, aspartame and neotame;
(g) The fruit and vegetable powder is at least one of orange powder, lemon powder, aloe drying powder, cherry powder, apple powder and strawberry powder;
(h) The essence is at least one of orange essence, lemon essence, cherry essence, apple essence and strawberry essence;
(i) The glidant is at least one of silicon dioxide, magnesium stearate and stearic acid.
Preferably, the preparation is one of oral liquid, solid beverage, granules, capsules, tablets and powder.
The invention provides a product which comprises the following components in parts by weight: 1-15 parts of the composition, 5-30 parts of dietary fiber, 2-10 parts of compound nutrient, 2-10 parts of amino acid, 40-80 parts of filler, 0-5 parts of sour additive, 0-5 parts of sweetener, 3-20 parts of fruit and vegetable powder and 0.1-5 parts of essence.
Preferably, the product is powder and comprises the following components in parts by weight: 8-16 parts of fructo-oligosaccharide, 2-6 parts of taurine, 0.5-3 parts of vitamin C, 0.5-5 parts of royal jelly freeze-dried powder, 0.5-5 parts of wall-broken rape pollen, 0.5-3 parts of aloe dried powder, 0.5-5 parts of aloe gel powder, 1-5 parts of compound nutrient, 0.5-5 parts of turmeric, 30-50 parts of erythritol, 20-35 parts of fructose, 2-6 parts of orange powder, 0.5-3 parts of orange essence and 2-6 parts of lemon powder.
The invention provides a preparation method when the product is powder, which comprises the following steps:
(1) Weighing the components according to the proportion;
(2) Mixing wall-broken rape pollen, lyophilized royal jelly powder, aloe gel powder and Curcuma rhizome uniformly to obtain the composition;
(3) And (3) uniformly mixing the aloe dried powder, the vitamin C, the compound nutrient, the fructo-oligosaccharide, the taurine, the erythritol, the fructose, the orange powder, the orange essence, the lemon powder and the composition prepared in the step (2) to obtain the product.
Before the preparation method is carried out, the quality standard of the used raw materials needs to be checked, the raw materials are moved into a clean area after being checked to be qualified (the cleanliness of the production environment of the clean area is 10 ten thousand grade, organization production is arranged according to GMP requirements, the temperature of a workshop is less than or equal to 25 ℃, the relative humidity is less than or equal to 65 percent), the wall-broken rape pollen, the aloe gel powder and the fructo-oligosaccharides are sieved by a vibrating screen for 20 meshes for later use, and then the raw materials are weighed. Preferably, in the step (2), the specific way of uniformly mixing the wall-broken rape pollen, the royal jelly freeze-dried powder, the aloe gel powder and the turmeric is as follows: putting the above raw materials into a hopper, placing the hopper into an automatic lifting hopper mixer, and mixing at the rotation speed of 10-15rpm for 15-25min. Preferably, in the step (3), the specific manner of mixing uniformly is: feeding into a feeder with 20 mesh screen, feeding into a mixing hopper via a vacuum system, and starting a double-shaft paddle mixer at 35-45rpm for mixing for 140-160s.
Preferably, when the preparation formulation prepared by the invention is powder, the packaging specifications are two types: 5 g/bag or 145 g/bottle.
Preferably, the product is a tablet, comprising the following components in parts by weight: 15-30 parts of fructo-oligosaccharide, 5-10 parts of taurine, 1-5 parts of vitamin C, 0.5-5 parts of royal jelly freeze-dried powder, 0.5-5 parts of wall-broken rape pollen, 0.5-3 parts of aloe dried powder, 0.5-5 parts of aloe gel powder, 3-8 parts of compound nutrient, 0.5-5 parts of turmeric, 30-50 parts of sorbitol, 5-20 parts of isomaltitol, 1-3 parts of orange powder, 0.5-2 parts of orange essence, 1-3 parts of lemon powder, 0.1-1 part of magnesium stearate and 0.1-1 part of silicon dioxide.
The invention provides a preparation method of a tablet, which comprises the following steps:
(1) Weighing the components according to the proportion;
(2) Mixing wall-broken rape pollen, lyophilized royal jelly powder, aloe gel powder and Curcuma rhizome uniformly to obtain the composition;
(3) And (3) uniformly mixing the aloe dried powder, vitamin C, compound nutrients, fructo-oligosaccharide, taurine, orange powder, orange essence, lemon powder, magnesium stearate, silicon dioxide, sorbitol and isomaltitol to obtain the composition prepared in the step (2), and tabletting to obtain the product.
Before the preparation method is carried out, the quality standard of the used raw materials needs to be checked, the raw materials are moved into a clean area after being checked to be qualified (the cleanliness of the production environment of the clean area is 10 ten thousand grade, tissue production is arranged according to GMP requirements, the temperature of a workshop is less than or equal to 25 ℃, the relative humidity is less than or equal to 65%), vitamin C, orange powder and orange essence are crushed, aloe gel powder and fructo-oligosaccharide are sieved by a vibrating sieve with 20 meshes, and wall-broken rape pollen is sieved by a vibrating sieve with 40 meshes for standby, and then weighing is carried out. Preferably, in the step (2), the specific way of uniformly mixing the wall-broken rape pollen, the royal jelly freeze-dried powder, the aloe gel powder and the turmeric is as follows: putting the above raw materials into a hopper, placing the hopper into an automatic lifting hopper mixer, and mixing at the rotation speed of 10-15rpm for 15-25min. Preferably, in the step (3), the specific manner of mixing uniformly is as follows: feeding into a feeder with 20 mesh screen, feeding into a mixing hopper through a vacuum system, starting a double-shaft paddle mixer, rotating at 50-60rpm, and mixing for 140-160s.
Preferably, when the preparation formulation prepared by the invention is a tablet, the tablet is tabletted by a tablet machine, the tablet weight is controlled to be 1.0g, and the packaging specification adopts 90 tablets/bottle.
Compared with the prior art, the invention has the beneficial effects that: evaluation tests show that common components (such as wall-broken rape pollen, royal jelly freeze-dried powder, aloe gel powder and turmeric) are combined and screened, and aloe polysaccharide in the aloe powder, flavone in the wall-broken rape pollen, protein in the royal jelly freeze-dried powder and polyphenol substances in the turmeric in the composition exert various regulation effects on an organism immune system by acting on immune organs (thymus and spleen), activating immune cells (lymphocytes, mononuclear phagocytes and white cells), regulating the release of cell factors, promoting the generation of antibodies and the like, so that the synergistic effect is achieved, and the integral immunity of the organism is improved. Meanwhile, under the condition of improving the whole immunity of the organism, the composition plays a synergistic role, reduces the occurrence of oxidative stress reaction, inhibits inflammatory mediators and transcription factors, and plays a better anti-inflammatory role, thereby reducing the occurrence of chronic diseases. The composition provided by the invention has good immune and anti-inflammatory effects, and the functional application of the product is expanded.
Detailed Description
To better illustrate the objects, technical solutions and advantages of the present invention, the present invention will be further described with reference to specific examples.
In the examples, the experimental methods used were all conventional methods unless otherwise specified, and the materials, reagents and the like used were all commercially available; the raw materials used in the examples and comparative examples of the present invention were derived as follows:
aloe gel powder, purchased from wanlv bio-inc, yunnan; lyophilized royal jelly powder purchased from Tianchu honey source in Hangzhou province; broken wall rape pollen, purchased from Qinghai Kangpu Biotech GmbH; turmeric, purchased from india.
Examples 1 to 5 and comparative examples 1 to 6
Example 1
The composition of the present example comprises the following components: 21.43 parts of aloe gel powder, 28.57 parts of royal jelly freeze-dried powder, 28.57 parts of wall-broken rape pollen and 21.43 parts of turmeric.
The preparation method of the composition provided by the embodiment of the invention comprises the following steps: mixing Aloe gel powder, lac Regis Apis lyophilized powder, cell wall broken bird rape pollen and Curcuma rhizome uniformly to obtain the composition with immunity enhancing and antiinflammatory functions.
Example 2
The difference between this example and example 1 is only that the composition of this example has the following components: 10 parts of aloe gel powder, 40 parts of royal jelly freeze-dried powder, 40 parts of wall-broken rape pollen and 10 parts of turmeric.
Example 3
The difference between this example and example 1 is only that the composition of this example comprises the following components: 15 parts of aloe gel powder, 35 parts of royal jelly freeze-dried powder, 35 parts of wall-broken rape pollen and 15 parts of turmeric.
Example 4
The difference between this example and example 1 is only that the composition of this example comprises the following components: 25 parts of aloe gel powder, 25 parts of royal jelly freeze-dried powder, 25 parts of wall-broken rape pollen and 25 parts of turmeric.
Example 5
The difference between this example and example 1 is only that the composition of this example has the following components: 30 parts of aloe gel powder, 20 parts of royal jelly freeze-dried powder, 20 parts of wall-broken rape pollen and 30 parts of turmeric.
The raw materials and the weight parts of the concrete comparative examples 1-6 are selected as follows:
comparative example 1
This comparative example differs from example 1 only in that the composition comprises the following components: 5 parts of aloe gel powder, 15 parts of royal jelly freeze-dried powder, 50 parts of wall-broken rape pollen and 30 parts of turmeric.
Comparative example 2
This comparative example differs from example 1 only in that the composition comprises the following components: 40 parts of aloe gel powder, 10 parts of royal jelly freeze-dried powder, 10 parts of wall-broken rape pollen and 40 parts of turmeric.
Comparative example 3
This comparative example differs from example 1 only in that the composition comprises the following components: 5 parts of aloe gel powder, 15 parts of royal jelly freeze-dried powder, 15 parts of wall-broken rape pollen and 65 parts of turmeric.
Comparative example 4
This comparative example differs from example 1 only in that the composition comprises the following components: 30 parts of aloe gel powder, 20 parts of wall-broken rape pollen and 50 parts of turmeric.
Comparative example 5
This comparative example differs from example 1 only in that the composition comprises the following components: 15 parts of aloe gel powder, 35 parts of royal jelly freeze-dried powder and 50 parts of turmeric.
Comparative example 6
This comparative example differs from example 1 only in that the composition comprises the following components: 25 parts of royal jelly freeze-dried powder, 25 parts of broken rape pollen and 50 parts of turmeric.
Application example 1
The powder comprises the following components in percentage by mass: 15% of fructo-oligosaccharide, 3% of taurine, 1% of vitamin C, 2% of royal jelly freeze-dried powder, 2% of wall-broken rape pollen, 0.8% of whole-leaf aloe oven-dried powder, 2% of aloe gel powder, 4% of compound nutrient, 1% of turmeric, 37.2% of erythritol, 25% of fructose, 3% of orange powder, 1% of orange essence and 3% of lemon powder.
Application example 2
A tablet comprises the following components in percentage by mass: 20% of fructo-oligosaccharide, 5% of taurine, 1.5% of vitamin C, 3% of royal jelly freeze-dried powder, 2% of wall-broken rape pollen, 1% of aloe whole leaf dry powder, 2% of aloe gel powder, 6% of compound nutrient, 2% of turmeric, 40% of sorbitol, 12.8% of isomaltitol, 2% of orange powder, 0.5% of orange essence, 1% of lemon powder, 0.8% of magnesium stearate and 0.4% of silicon dioxide.
Effect verification
Test example 1 improved T cell reduction.
The experimental process comprises the following steps: 4dpf transgenic T cell red fluorescent zebra fish is randomly selected to be placed in a 6-well plate, and 30 zebra fish are processed in each well. The samples were separately administered in water, while a positive control (bailing capsule), a normal control group and a model control group were set, each well having a volume of 3mL. Except for a normal control group, the other experimental groups are injected with vinorelbine tartrate injection solution intravenously to establish a zebra fish T cell reduction model.
After treatment for 1 day at 28 ℃, 8 zebra fish are randomly selected from each experimental group and placed under a fluorescence microscope for photographing, the fluorescence intensity of the zebra fish T cells is analyzed, and the T cell reduction improving effect of the sample is evaluated according to the statistical analysis result of the index. Statistical treatment results are expressed as mean SE. Statistical analysis was performed using the independent T-test, with p <0.05 indicating that the differences were statistically significant.
And (3) test results: as shown in tables 1 and 2.
TABLE 1
Figure BDA0003786762230000091
TABLE 2
Figure BDA0003786762230000092
P <0.05, p <0.01, p <0.001, compared to model controls.
As can be seen from the above table, examples 1 to 5 have differences (p < 0.05) compared with the model control group, which indicates that the effect of improving T cell reduction is achieved, examples 1 and 2 have significant differences (p < 0.01) and better effect of improving T cell reduction compared with the model control group, and example 3 has very significant differences (p < 0.001) and best effect of improving T cell reduction compared with the model control group. Comparative examples 1-6 showed no significant difference from the model control group, indicating no improvement in T cell reduction function.
Test example 2 improved macrophage reduction.
The test process comprises the following steps: randomly selecting 3dpf transgenic macrophage green fluorescent strain zebra fish in a 6-well plate, and treating 30 zebra fish in each well. The samples were separately administered in water, while a positive control (bailing capsule), a normal control group and a model control group were set, each well having a volume of 3mL. Except for a normal control group, the other experimental groups are injected with vinorelbine tartrate injection in a vein to establish a zebra fish macrophage reduction disease model. The solution was changed once at 5 dpf.
After treatment for 4 days at 28 ℃, 5 zebra fish are randomly selected from each experimental group and placed under a fluorescence microscope for photographing, the fluorescence intensity of the zebra fish macrophages is analyzed, and the macrophage reduction efficacy of the sample is evaluated according to the statistical analysis result of the index. Statistical treatment results are expressed as mean SE. Statistical analysis was performed using the independent T-test, with p <0.05 indicating that the differences were statistically significant.
And (3) test results: as shown in tables 3 and 4.
TABLE 3
Figure BDA0003786762230000101
TABLE 4
Figure BDA0003786762230000102
P <0.05, p <0.01, p <0.001, compared to model controls.
As can be seen from the above table, examples 1 to 5 have differences (p < 0.05) compared with the model control group, which indicates that the function of improving macrophage reduction is provided, examples 1 and 2 have significant differences (p < 0.01) and better effect of improving macrophage reduction compared with the model control group, example 3 has very significant differences (p < 0.001) and optimal effect of improving macrophage reduction compared with the model control group, and comparative examples 1 to 6 have no significant difference compared with the model control group, which indicates that the function of improving macrophage reduction is not provided.
Test example 3 reduced the amount of NO secretion.
The test mechanism is as follows: macrophages play a mediating role in the inflammatory response process, and activated proinflammatory factors such as TNF-alpha, proinflammatory mediators NO and the like can induce and strengthen local inflammatory response. NO is mainly produced by nitric oxide synthase catalysis, when macrophages are stimulated by LPS and other substances, the expression level of iNOS is increased, the generation of a large amount of inducible NO is catalyzed in a short time, and the increase of the level of NO can enable cells to release active oxygen intermediates and inflammatory cytokines and further lead to the large-amount synthesis of NO. Lipopolysaccharide LPS induced RAW264.7 mouse macrophage model anti-inflammatory test is widely applied to screening whether a test object has anti-inflammatory effect. Therefore, NO can be used as an index for evaluating the anti-inflammatory efficacy of the test substance.
By 1.5X 10 5 Inoculation of cells into 24-well plates at an inoculation density of one/mL, 800. Mu.L cell suspension per well, incubator (37 ℃, 5% CO) 2 ) And incubated overnight. The test set up blank control group, positive control group (dexamethasone) and sample group. When the cell plating rate in the 24-well plate reaches 50% -60%, the administration is carried out in groups, the dose per well is 800 μ L, each group is provided with 3 multiple wells, the culture box (37 ℃, 5% CO) 2 ) The cultivation was continued for 2h. After 2h of incubation, LPS working solution prepared from the corresponding test substance working solution was added to the dosed well plate according to the group design, the well plate was shaken from side to mix the drug in the well plate to a final concentration of 1. Mu.g/mL LPS, and the incubator (37 ℃, 5% CO) 2 ) The culture was continued for 24h. Transferring cell supernatant of each well into a new 96-well plate (50 muL/well), adding 50 muL of Griess reagent I, then 50 muL of Griess reagent II, after reaction of each well is completed, uniformly mixing, measuring a light absorption value on an enzyme labeling instrument at 540nm, and calculating corresponding NO concentration on a standard curve according to the light absorption value of a sample.
And evaluating the anti-inflammatory efficacy of the sample to be tested by detecting the secretion condition of the inflammatory factor NO. When statistical analysis is performed by the t-test method.
And (3) test results: as shown in tables 5 and 6.
TABLE 5
Figure BDA0003786762230000111
TABLE 6
Figure BDA0003786762230000112
Comparison of sample group with LPS group (model group) P <0.05, P < 0.01.
As can be seen from the above table, the data of examples 1 to 5 are different in comparison with the model control group, which indicates that the effect of reducing the amount of NO secretion is achieved, examples 1, 2 and 3 are significantly different and have better effect of reducing the amount of NO secretion in comparison with the model control group, and comparative examples 1 to 6 are not significantly different and do not have the effect in comparison with the model control group.
Test example 4 reduced TNF-. Alpha.secretion.
Test standards: macrophages play a mediating role in the inflammatory response, and activated proinflammatory factors such as TNF-alpha, proinflammatory mediators NO and the like can induce and strengthen local inflammatory response. The TNF-alpha released in a proper amount can mediate immune response and has important regulation effect on body metabolism and the like, and a large amount of TNF-alpha released can cause an amplification reaction of inflammation. Lipopolysaccharide LPS induced RAW264.7 mouse macrophage model anti-inflammatory test is widely applied to screening whether a test object has anti-inflammatory effect. Therefore, TNF-alpha can be used as an index for evaluating the anti-inflammatory efficacy of the test substance.
Cells were seeded at a seeding density of 1.5X 105/mL into 24-well plates at 800. Mu.L cell suspension per well, incubator (37 ℃, 5% CO) 2 ) And incubated overnight. The test set up blank control group, positive control group (dexamethasone) and sample group. When the cell plating rate in the 24-well plate reached 50% -60%, the administration was carried out in groups, the dose per well was 800. Mu.L, each group was provided with 3 multiple wells, and the incubator (37 ℃, 5% CO) 2 ) The cultivation was continued for 2h. After 2h of incubation, LPS working solution prepared from the corresponding test substance working solution was added to the dosed well plate according to the group design, the well plate was shaken from side to mix the drug in the well plate to a final concentration of 1. Mu.g/mL LPS, and the incubator (37 ℃, 5% CO) 2 ) After further culturing for 24h, collecting cell culture supernatant in an EP tube, after collection, placing a sample for TNF-alpha content detection in a refrigerator at-80 ℃ for freezing storage, and executing a specific TNF-alpha test method according to the instruction of the kit.
And evaluating the anti-inflammatory efficacy of the sample to be tested by detecting the secretion condition of the inflammatory factor TNF-alpha. When statistical analysis is performed using the t-test method.
And (3) test results: as shown in table 7 and 8.
TABLE 7
Figure BDA0003786762230000121
TABLE 8
Figure BDA0003786762230000122
Figure BDA0003786762230000131
The sample group was compared to the LPS group (model group) × P <0.05, and × P < 0.01.
As can be seen from the above table, the data of examples 1 to 5 are different from those of the model control group, which indicates that the compound has the effect of reducing the TNF- α secretion (anti-inflammatory), examples 1, 2 and 3 have significant difference from the model control group, and the effect of reducing the TNF- α secretion is better, and comparative examples 1 to 6 have no significant difference from those of the model control group, and do not have the effect.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims (15)

1. A composition with immunity enhancing and anti-inflammatory functions is characterized by comprising the following components: aloe powder, lyophilized royal jelly powder, wall-broken rape pollen, and Curcuma rhizome; wherein, the weight ratio of the aloe powder, the royal jelly freeze-dried powder, the wall-broken rape pollen and the turmeric is as follows: aloe powder: lyophilized royal jelly powder: breaking cell walls of rape pollen: turmeric = (10-30): (20-40): (20-40): (10-30).
2. The composition with immunity enhancing and anti-inflammatory functions as claimed in claim 1, wherein the weight ratio of aloe powder, royal jelly freeze-dried powder, wall-broken rape pollen and turmeric is as follows: aloe powder: lyophilized royal jelly powder: wall breaking of rape pollen: turmeric = (10-23): (27-40): (27-40): (10-23).
3. The composition with immunity enhancing and anti-inflammatory effects of claim 2, wherein the weight ratio of aloe powder, lyophilized royal jelly powder, broken rape pollen and turmeric is as follows: aloe powder: lyophilized royal jelly powder: breaking cell walls of rape pollen: turmeric =15:35:35:15.
4. the composition with the functions of enhancing immunity and resisting inflammation as claimed in any one of claims 1-3, wherein the aloe powder is aloe gel powder, and the mass percentage content of polysaccharide in the aloe gel powder is more than or equal to 80000mg/kg; in the aloe gel powder, the mass percentage of O-acetyl is more than or equal to 75000mg/kg calculated by acetylcholine chloride.
5. A method for preparing the composition with immunity enhancing and anti-inflammatory functions as claimed in any one of claims 1 to 4, comprising the steps of: mixing Aloe powder, lac Regis Apis lyophilized powder, cell wall broken rape pollen and Curcuma rhizome uniformly to obtain the composition with immunity enhancing and antiinflammatory functions.
6. Use of the composition according to any one of claims 1 to 4 for the preparation of a preparation or food product having an immunostimulating and anti-inflammatory effect.
7. An article of manufacture comprising the composition of any one of claims 1-4 having an immune enhancing and anti-inflammatory effect.
8. The product of claim 7, wherein the components of the product further comprise an adjuvant, and the adjuvant is at least one of dietary fiber, compound nutrient, amino acid, filler, sour agent, sweetener, fruit and vegetable powder, essence, and glidant.
9. The article of claim 8, wherein at least one of (a) - (i) below:
(a) The dietary fiber is at least one of fructo-oligosaccharide, xylo-oligosaccharide, galacto-oligosaccharide, isomalto-oligosaccharide, inulin and resistant dextrin;
(b) The compound nutrient is a mixture of nutrient and mineral; the nutrient is at least one of vitamin C, vitamin E, vitamin A and vitamin D; the mineral substance is at least one of calcium salt, magnesium salt, zinc salt, chromium salt and iron salt;
(c) The amino acid is at least one of taurine, alanine, arginine, leucine, isoleucine and valine;
(d) The filler is at least one of erythritol, fructose, maltitol, sorbitol, isomalt, xylitol and lactose;
(e) The sour agent is at least one of citric acid, malic acid, lactic acid, fumaric acid and tartaric acid;
(f) The sweetener is at least one of stevioside, mogroside, sucralose, aspartame and neotame;
(g) The fruit and vegetable powder is at least one of orange powder, lemon powder, aloe drying powder, cherry powder, apple powder and strawberry powder;
(h) The essence is at least one of orange essence, lemon essence, cherry essence, apple essence and strawberry essence;
(i) The glidant is at least one of silicon dioxide, magnesium stearate and stearic acid.
10. The preparation according to any one of claims 7 to 9, wherein the preparation is in the form of one of oral liquid, solid beverage, granules, capsules, tablets and powders.
11. The product is characterized by comprising the following components in parts by weight: 1-15 parts of the composition of any one of claims 1-5, 5-30 parts of dietary fiber, 2-10 parts of compound nutrient, 2-10 parts of amino acid, 40-80 parts of filler, 0-5 parts of sour agent, 0-5 parts of sweetener, 3-20 parts of fruit and vegetable powder and 0.1-5 parts of essence.
12. The article of claim 11, wherein the article is a powder comprising the following components in parts by weight: 8-16 parts of fructo-oligosaccharide, 2-6 parts of taurine, 0.5-3 parts of vitamin C, 0.5-5 parts of royal jelly freeze-dried powder, 0.5-5 parts of wall-broken rape pollen, 0.5-3 parts of aloe dried powder, 0.5-5 parts of aloe gel powder, 1-5 parts of compound nutrient, 0.5-5 parts of turmeric, 30-50 parts of erythritol, 20-35 parts of fructose, 2-6 parts of orange powder, 0.5-3 parts of orange essence and 2-6 parts of lemon powder.
13. A method of making the article of claim 12, comprising the steps of:
(1) Weighing the components according to the proportion;
(2) Mixing wall-broken rape pollen, lyophilized royal jelly powder, aloe gel powder and Curcuma rhizome uniformly to obtain the composition;
(3) And (3) uniformly mixing the aloe dried powder, vitamin C, compound nutrients, fructo-oligosaccharide, taurine, erythritol, fructose, orange powder, orange essence, lemon powder and the composition prepared in the step (2) to obtain the product.
14. The article of claim 11, wherein the article is a tablet comprising the following components in parts by weight: 15-30 parts of fructo-oligosaccharide, 5-10 parts of taurine, 1-5 parts of vitamin C, 0.5-5 parts of royal jelly freeze-dried powder, 0.5-5 parts of wall-broken rape pollen, 0.5-3 parts of aloe dried powder, 0.5-5 parts of aloe gel powder, 3-8 parts of compound nutrient, 0.5-5 parts of turmeric, 30-50 parts of sorbitol, 5-20 parts of isomaltitol, 1-3 parts of orange powder, 0.5-2 parts of orange essence, 1-3 parts of lemon powder, 0.1-1 part of magnesium stearate and 0.1-1 part of silicon dioxide.
15. A method of making the article of claim 14, comprising the steps of:
(1) Weighing the components according to the proportion;
(2) Mixing wall-broken rape pollen, lyophilized royal jelly powder, aloe gel powder and Curcuma rhizome uniformly to obtain the composition;
(3) And (3) uniformly mixing the royal jelly freeze-dried powder, the aloe dried powder, the vitamin C, the compound nutrient, the fructo-oligosaccharide, the taurine, the orange powder, the orange essence, the lemon powder, the magnesium stearate, the silicon dioxide, the sorbitol and the isomaltitol, and the composition prepared in the step (2), and tabletting to obtain the product.
CN202210952876.0A 2022-08-08 2022-08-08 Composition with immunity enhancing and anti-inflammatory functions and preparation method thereof Pending CN115363205A (en)

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Application publication date: 20221122