JPS61194032A - Pulverized coriolus versicolor containing large amount of ganoderic acid as useful component, and production thereof - Google Patents

Abstract

PURPOSE:To provide a pulverized REISHI (Coriolus versicolor) containing a large amount of ganoderic acid. CONSTITUTION:REISHI [fungus belonging to Polyporaceae family, e.g. SEKISHI, KOKUSHI, HAKUSHI, OUSHI, SHISHI, etc. (all are kinds of REISHI classified by colors) is dried, cut in the form of mince, frozen at -10--80 deg.C, and crushed with a crusher such as ball mill in frozen state. The crushed product is taken out of the mill, returned to normal temperature, and sieved to obtain the powder of 10-200 mesh. The fraction left on a 10-mesh sieve is frozen again and crushed with a ball mill. The fraction of 10-200 mesh is sterilized at a low temperature (<=60 deg.C) to obtain the crushed REISHI containing a large amount of ganoderic acid of formula as an active component. The compound of formula effectively inhibits the activity of cyclooxynase which is an arachidonic acid metabolism enzyme. EFFECT:The sensible threshold of bitter taste with the tongue can be lowered, and the dose can be decreased.

Description

DETAILED DESCRIPTION OF THE INVENTION (Technical field to which the invention pertains) This invention relates to a crushed Ganoderic acid product containing a large amount of useful ingredients ganoderic acids and a method for producing the same, and particularly relates to a crushed Ganoderma product containing a large amount of useful ingredients ganoderic acids and a method for producing the same. To provide a crushed Reishi mushroom that has the particle size of mesh, is drinkable directly without adhesion of bacteria, and contains a large amount of useful ingredients ganoderic acids with a high intake rate of the active ingredients ganoderic acids, and a method for producing the same. .

(Prior Art and Its Disadvantages) In general, Ganoderma lucidum is widely known to have many useful medicinal ingredients such as polysaccharides, but on the other hand, Ganoderma lucidum is so viscous that it cannot be crushed with a normal pulverizer. Hot water extract (decoction)
The extract was applied in the form of spray-dried granules, etc.

Generally, polysaccharides and saponins are water-soluble, and the polysaccharides of Reishi mushrooms have long been well known as active ingredients, and it has been said that Reishi mushrooms should be taken after hot water extraction.

However, in practice, it has been empirically shown that the components that can be eluted through extraction are limited, and folklore has it that it is more effective to crush Reishi mushrooms and drink them by some means than to decoct them. It was said that

However, due to the unique logic of scholars who reject folklore as unscientific, and because the active ingredient was not found in the water-insoluble fraction, until now only the hot water extract of Reishi was used. It is something.

In addition, although Reishi mushrooms have useful medicinal properties, they generally have the disadvantage of being difficult to take or drink on a regular basis.

The reason for this is that Reishi mushrooms contain bitter glycosides, which are easily extracted by hot water.

In addition, as mentioned above, reishi has a high viscosity, so it cannot be crushed with a normal crusher, and even if it is crushed, it will be moxa-like (cotton-like).
The above-mentioned reason that it is a pulverized product and cannot be taken directly was also the basis for using only hot water extracts that can be boiled and drunk.

(Problem to be solved) However, this inventor has been researching Reishi mushrooms for many years, and in the process he has continued to conduct research to scientifically confirm the folklore, and in the process has not only revealed the effectiveness of Reishi mushrooms but also many of them. Free triterpenes (ganoderic acids), which are found in large amounts in sugars but are water-insoluble bitter components, are very effective.Unlike bitter glycosides, which are glycoside triterpenes, these free triterpenes (ganoderic acids) are non-water-soluble. Because it is a gender,
Rather, it is better to directly drink the crushed product because you can reliably ingest ganoderic acids along with polysaccharides, and the contact time between your tongue and bitter components or bitter glycosides during the day is shortened, so you can suppress bitterness, and furthermore, you can enjoy the effects of Reishi. For the first time, I discovered that I was able to demonstrate my abilities more effectively.

At the same time, the inventor believes that if Reishi is crushed, the bitter components and bitter glycosides will be present in the Reishi cells, and most of them will not come into direct contact with the tongue in the mouth, thus reducing the bitter taste of Reishi. We focused on the fact that the detection by the tongue can be reduced, and the negative effects such as the decomposition of the active ingredient by peptides and gastric acid in gastric fluid can be eliminated.

However, as mentioned above, reishi mushrooms cannot be ground with a regular grinder because of their high viscosity. Otherwise, none of the above drawbacks could be overcome.

(Solution Means) This invention was made to achieve the above-mentioned problems.
A crushed Reishi mushroom containing a large amount of the useful ingredient ganoderic acid (formula (1) below) with a particle size of 200 mesh and freeze-pulverized Reishi mushroom to a particle size of 10 to 200 mesh, and then freeze-pulverize this crushed product at a low temperature of 60°C or less. The present invention relates to a method for producing crushed Ganoderic acid containing a large amount of the useful ingredient ganoderic acids (there are 6 types as shown in the following formula (1)), which is sterilized by sterilization.

The reishi mushroom used in this invention is a mushroom that belongs to the family Monkeyaceae, and is generally called Mannentake, and its scientific name is GARODERMA lucidamum callusp.
MA LUCIDUMUM KARSP) and good red grass,
There are varieties such as black grass, white grass, young grass, and purple grass, and any of these known reishi mushrooms can be suitably used in the present invention.

To obtain such a granular pulverized product of Reishi, first dry the Reishi, cut the dried product into minced pieces, freeze the cut product at below -10°C, and process it in a ball mill etc. in this frozen state. Grind with a grinder.

In this invention, the freezing temperature ranges from -10°C to -18°C.
A temperature in the range of 0° C. can be adopted, and the dried Ganoderma lucidum does not need to be cut into minced pieces as described above to be subjected to pulverization, and in some cases, it may be pulverized as it is.

After this pulverization, the pulverized material is taken out, returned to room temperature, and then passed through a sieve, and only those with a mesh size of 10 to 200 are taken out.

Those that do not pass 10 mesh are frozen again and ground in a ball mill.

In this invention, the pulverizer is not limited to a ball mill, and other conventional mechanical pulverizers can be used.

Thereafter, only the crushed material having a particle size of 10 to 200 mesh is collected and sterilized at low temperature.

In this invention, the reason for sterilizing at low temperature is to avoid the active ingredients such as ganoderic acids from disappearing due to thermal decomposition as mentioned above, and the reason why sterilization is necessary is to prevent the active ingredients such as ganoderic acids from disappearing due to thermal decomposition. This pasteurization step is an essential step in this invention because it eliminates bacteria, insect eggs, and other substances that are undesirable for human consumption.

The sterilization method is not particularly limited, and commonly known methods such as ultraviolet sterilization and intermittent low-temperature heating sterilization can be employed.

This pulverized product can be made into a powder as it is, or it can be added with other commonly known excipients, fillers, additives, flavorings, sweeteners, etc., as well as other medicinal ingredients as required. It may be prepared in the form of powder, tablet, granule, pill, syrup, soft drink, etc.

(Effects of the Invention) As detailed above, the crushed reishi mushroom product and the method for producing the same according to the present invention are manufactured using reishi mushrooms containing a large amount of useful ingredient ganoderic acids (formula (l) above) having a particle size of at least 10 mesh to 200 mesh. A method for producing a crushed reishi product containing a large amount of useful ingredients ganoderic acid, which consists of freeze-pulverizing the crushed product and reishi to a particle size of 10 to 200 mesh, and sterilizing the crushed product at a low temperature of 60°C or lower. Because it is a substance, when reishi that has a high viscosity is crushed as in the past, it becomes moxa-like (cotton-like).
This eliminates the disadvantage of being unable to take it directly as a pulverized product, and it is possible to reliably make it into a granular pulverized product. can be taken out only in the body, and because it is a pulverized product, the amount of bitter glycosides that are water-soluble substances that are detected and come into contact with the tongue in the mouth is small (therefore, the degree of bitterness that is perceived by the tongue can be reduced, and the extract It also has the effect that the amount used can be as small as about 1/2 to 173 to achieve the same effect.

The effects of this invention will be explained in detail below by further illustrating test examples of this invention.

Test Examples 1 to 4 100 g of Ganoderma lucidum was cut into minced pieces (about 5 mm in size), frozen at -30° C., and the frozen raw material was subjected to a ball mill to obtain 80 mesh passes of powdered Ganoderma lucidum.

This pulverized material was sterilized by ultraviolet light for 5 minutes.

This granular material was made into an aqueous solution at the concentrations shown in Table 1.

Comparative Example An aqueous solution made from 100g of dried Reishi and extracted water of 11
An aqueous solution diluted 0 times was obtained.

(Comparative Example 1) Healthy drinking water having such a composition was sampled by 20 people, and Table 2 shows the proportion of those 20 people who felt bitterness and the proportion who did not.

In addition, the ○ mark indicates the number of people who feel bitterness, the Δ mark indicates slightly bitterness, and the X mark indicates the number of people who do not feel bitterness at all.

The results are summarized in Table 2.

1st Table 1 1 grain weight % 1 1 test example 112.01 1-215.01 1 ' 3 110.0 1 1 - 4 120.0 1 2nd table 1101Δ1×1 1 test example 110112181 1 #210113 + 71 1 〃4+1111181 1 Comparative Example 111911101 From these results, it can be seen that most people do not feel much bitterness from the crushed Reishi mushroom according to the present invention.

Test Examples 5 to 8 and Comparative Example 2 100 g of Ganoderma lucidum was first dried, and the dried product was ground into minced pieces (5
Cut into pieces (about the size of +a+) and heat at the temperature shown in Table 3.
Saved time.

Next, this frozen raw material was placed in a ball mill for 30 minutes, and the state of passage through the sieve was observed.

I got powdered Reishi.

This pulverized material was sterilized with ultraviolet light three times intermittently for 5 minutes except for Comparative Example 2.

Table 4 shows the maximum particle size distribution of this powder material having a particle size distribution of 30% or more and the number of bacteria (such as Escherichia coli).

(Margin below) 3rd Table 1 1 Temperature ℃ 1 1 Test example 51-801 1 61-301 1 71-101 1 810.01 1 Comparative example 2 120.0 1 4th Table 1 1 Maximum distribution particle size III Bacterial count 11 test examples 511
40~160 1 0 11 〃61 100~12
0 + 0 11 〃 7180~100
1 0 11 #8140-60101 1 Comparative Example 21 10 on 15X101 Analysis Test The solvent of the Reishi mushroom powder of Test Example 1 and the extract of Comparative Example 1 was distilled off under reduced pressure, and then the powder was freeze-dried to form a powder.

Using 500 g of this powder, it was temporarily extracted with acetone, the solvent was distilled off under reduced pressure, and then this extract was fractionated by silica gel column chromatography using chloroform-ethanol (10:1) as a developing solvent, and identified using a standard substance. , carried out qualitative and quantitative analysis of ganoderic acids.

As a result, triterpenes were obtained from Test Example 1 at a yield of 1χ.

In contrast, only 0.09χ could be isolated from Comparative Example 1.

In addition, after comparing ganoderic acids with standard products, 100-MHz'3C-NMR, 400-MHz-! H
-Identified by NMR and mass spectra. - As a result, in Test Example 1, the bitter component was ganoderin sour 85χ and the rest was glycosides, but in Comparative Example 1, all of the bitter components were triterpenes, which are glycosides.

The following test was conducted using the ganoderic acids obtained from Test Example 1.

Rat blood was collected from -star-King normal rats (2
50-350 g) with EDTA added as an anticoagulant (5.811 IM), the blood was
The supernatant (platelet-rich plasma) was further centrifuged at 1500×g for 10 minutes.

The precipitated platelets were treated with HEPfiS- containing 21 BGTAs.
Saline buffer (25d HEPES, 135mM
Nacj! Washed twice with pH 7,4) and suspended (2
~3 ■ protein/van).

The above-mentioned ganoderic acids were added to the washed platelet suspension at various concentrations shown in Table 5, and the mixture was pre-incubated at 37°C for 5 minutes.

Thereafter, [1-C]arachidonic acid (0.05 μCi) was added and incubated for 5 minutes.

The reaction is stopped with formic acid and the arachidone vinegar metabolite is I! to AC
Extracted with.

The extract was dried with N2 gas. Add a small amount of II to this! to
Add AC and perform silica gel thin layer chromatography (TL
To separate and quantify using C), developing solution: chloroform: methanol: acetic acid: water = 90:8:1:0.8 V/V)
.

Radioactive substances were detected by autoradiography, the spots were cut out, and the radioactivity was quantified using a liquid scintillation counter.

The results are summarized in Table 5.

(Margins below) 5 Table 1 1 Product (ratio χ with comparative example) 11
112-HETE ITXB low IHHT
11 1 ** 1100.0 1100.0
1100.0 11*I 101132.0 1
116.0 1112.0 11ga 11111 1no + 1001128.0 1101.0170.
0 11de 11111 1li I 5001164.5 166.4138.5
11+11 1 acid 110001214.5 133.2124.7
Class 11 +1111 *The unit of ganoderic acids indicates the concentration added to the washed platelet suspension (μg/10).

**Comparison example for concentration O As can be seen from this result, ganoderic acids, which are the bitter components of Reishi mushrooms, are active ingredients that effectively inhibit the activity of the arachidonic acid metabolizing enzyme cyclooxynase.
It can be said that the ground Ganoderma lucidum containing a large amount of useful ingredients ganoderic acid according to the present invention is extremely useful because it contains a large amount of water-insoluble ganoderic acid.

Claims (1)

[Scope of Claims] (1) A pulverized product of Reishi mushroom containing a large amount of useful ingredient ganoderic acids (formula (1) below) having a particle size of at least 10 mesh to 200 mesh. ▲There are mathematical formulas, chemical formulas, tables, etc.▼...(1) R1 R2 R3 R4 (1) = O ▲There are mathematical formulas, chemical formulas, tables, etc.▼ ▲There are mathematical formulas, chemical formulas, tables, etc.▼ H (2)▲ There are mathematical formulas, chemical formulas, tables, etc. ▼ ▲ There are mathematical formulas, chemical formulas, tables, etc. ▼ = O H (3) = O ▲ There are mathematical formulas, chemical formulas, tables, etc. ▼ ▲ There are mathematical formulas, chemical formulas, tables, etc. ▼ Me (4 ) = O ▲ There are mathematical formulas, chemical formulas, tables, etc. ▼ ▲ There are mathematical formulas, chemical formulas, tables, etc. ▼ Me (5) = O = O = O Me (6) ▲ There are mathematical formulas, chemical formulas, tables, etc. ▼ ▲ Mathematical formulas, There are chemical formulas, tables, etc. ▼ = O Me (2) Ganoderic acids (the above formula) are made by freeze-pulverizing Reishi to a particle size of 10 mesh to 200 mesh and sterilizing this crushed product at a low temperature of 60°C or less. A method for producing crushed Reishi mushrooms containing a large amount of (1)).