KR102545583B1 - Method for producing double-coating red ginseng granule with enhanced ginsenoside content and improved preservability - Google Patents

Abstract

The present invention relates to a method for producing double-coated red ginseng granules with enhanced ginsenoside content and preservation.
The double-coated red ginseng granules prepared according to the present invention have increased amounts of ginsenosides Rb1, Rg1 and Rg3, ginsenoside F2 and ginsenoside compound K, and improved preservation and enteric properties. In addition, the double-coated red ginseng granules have a high absorption rate of ginsenoside in the body, are easy to carry, and are easy to consume in a fixed amount, so they are very useful for improving the health of modern people.

Description

Method for producing double-coating red ginseng granule with enhanced ginsenoside content and improved preservability}

The present invention relates to a method for producing double-coated red ginseng granules with enhanced ginsenoside content and preservation.

Saponin is known as an effective component of ginseng and red ginseng. Saponin of ginseng and red ginseng is a type of glycoside and has a unique chemical structure different from saponin found in other plants, and its efficacy is also very different. Saponins of ginseng and red ginseng are called 'ginsenosides', meaning ginseng glycosides, to distinguish them from saponins of other plant origin.

With the recent development of analytical technology, the chemical structures of about 30 ginsenosides have been identified so far. This is far more than the 14 types of Western ginseng and 15 types of Chinese ginseng. Ginsenosides are based on glycosides with a dammarane backbone, and depending on the type and number of sugars attached to R1, R2, and R3 positions in their chemical structure, ginsenosides such as diols (PPD) and triols (PPT) classified as a side.

During processing, when high pressure is applied to these ginsenosides, enzymes are added, or heating is performed, some of the sugars are removed or new double bonds are formed, resulting in unique ginsenosides. Therefore, products containing various types of ginsenosides are being released according to the processing method of ginseng.

In order for ginseng saponin to be absorbed into the body, diol-type ginsenosides are 20(S)-protopanaxadiol 20-O-β-D-glucopyranoside (20(S)-protopanaxadiol 20-O-β-D- glucopyranoside, FGM 1, hereinafter referred to as 'Compound K'), and triol-based ginsenosides are decomposed into 20(S)-protopanaxatriol (20(S)-protopanaxatriol, FGM 4), It exerts its efficacy as it is absorbed, but only a small amount is converted by heat, acid, pressure, digestive enzymes, etc., and is finally absorbed into the body.

Recent studies have shown that gut microbes are required for the conversion of ginseng saponins into these end products. In order for saponin taken orally to be absorbed, it must be decomposed by intestinal microorganisms. Privotella oris is a representative bacterium that metabolizes saponin, and various beneficial bacteria such as lactic acid bacteria are involved in saponin metabolism.

In a study estimated by examining human consumption among the general population, 30% of normal people did not have any bacteria that could metabolize saponin, and although there was a difference in metabolic ability among the remaining 70%, both diol and triol saponins were normally absorbed. It turns out that only a few people can metabolize it. In particular, it has been reported that the rate of saponin metabolism is further reduced because the intestinal flora is not stable when continuous anticancer treatment or drugs are taken.

In this regard, it is highly desirable to develop a product that anyone can benefit from by completing fermentation with microorganisms from the outside and converting the active ingredients of ginseng or red ginseng into a form that can be absorbed regardless of the individual's metabolic function. can see.

On the other hand, far-infrared rays have a wavelength of about 3 to 1,000 μm, and are used as a method of heating and non-heating, and are applied to food ripening and flavor enhancement. Far-infrared rays are biologically active and have the property of evenly transferring heat to the center of a material (Inoue etal., 1989, Int. J. Biom. 33: 145-150), and due to this property, the heating time is shortened. By doing so, effects such as energy saving can be brought about, and loss of nutrients or physiologically active substances due to thermal decomposition can be optimized. Natural antioxidants have polymers such as polyphenol, tocopherol, and flavonoid, and it has been reported that far-infrared ray treatment liberates them into small molecules (Niwa etal., 1986, Inflammation. 10: 79-91). It has been reported that high-molecular polyphenols are liberated and antioxidant activity is increased (Lee et al., 2003, J. Agric. Food Chem. 51: 4400-4403).

Recently, as interest in health has increased, intake of various health functional foods such as ginseng and red ginseng, such as ginseng and red ginseng tea products, which have effects such as immunity enhancement and rejuvenation, has increased. However, in the case of existing commercially available red ginseng granules, the sugar content is very high and red ginseng components are added in small amounts, and during the process of manufacturing red ginseng granules, not only the content of ginsenosides decreases, but also the solubility is low, so it is always hot water during the drinking process. It had to be dissolved in, and there was the inconvenience of having to stir for a long time to dissolve.

As a method for improving this inconvenience, Korean Patent Registration No. 10-1603171 discloses a method for producing concentrated red ginseng granules with increased ginsenoside content. and ginsenoside compound K is not contained, and has a disadvantage of low preservability.

In the process of research to develop red ginseng granules that solve the above problems, the present inventors irradiated far-infrared rays in the process of first coating the red ginseng concentrate seed powder with the fermented red ginseng concentrate fermented by using enzymes and lactic acid bacteria, and red ginseng. The present invention was completed by specifically confirming that, in the case of secondary coating with a mixed solution of concentrate and natural polysaccharide, the content of ginsenosides including ginsenoside compound K was increased, and red ginseng granules with improved preservation and solubility were produced. did

KR 10-1603171 B

An object to be solved by the present invention is to provide a method for producing red ginseng granules with enhanced ginsenoside content and preservation.

In order to achieve the above object, the present invention provides (1) spray-drying red ginseng concentrate to prepare red ginseng concentrate seed powder; (2) preparing a first coating solution by mixing red ginseng concentrate, enzyme, dead lactic acid bacteria, live lactic acid bacteria, and purified water; (3) Put the red ginseng concentrate seed powder prepared in step (1) at the bottom of the fluidized bed coating granulator, and then inject heated air, and apply far-infrared rays at the top of the fluidized bed coating granulator. Range of 2 to 14 ㎛ shaping into granules by spraying the primary coating solution of step (2) through a spray nozzle at the bottom of the fluidized bed coating granulator while irradiating far-infrared rays emitting wavelengths of; (4) preparing a secondary coating solution by mixing red ginseng concentrate, natural polysaccharide, and purified water; And (5) the secondary coating solution of step (4) is injected through the spray nozzle at the bottom of the fluidized bed coating granulator while introducing heated air after introducing the granules prepared in step (3) to the bottom of the fluidized bed coating granulator. It provides a method for producing double-coated red ginseng granules with enhanced ginsenoside content and preservation, including the step of spraying and molding into double-coated granules.

According to one embodiment of the present invention, the red ginseng concentrate in step (1) may be irradiated with far-infrared rays emitting a wavelength in the range of 2 to 14 μm before or after spray drying.

According to one embodiment of the present invention, the enzyme in step (2) may be a complex enzyme in which protease and alpha-amylase are mixed in a weight ratio of 1:1-4.

According to one embodiment of the present invention, the lactic acid bacteria in step (2) may be one or more lactic acid bacteria selected from among Lactobacillus, Bifidobacterium and Streptococcus.

According to one embodiment of the present invention, the lactic acid bacteria are Lactobacillus plantarum , Lactobacillus rhamnosus , Bifidobacterium lactis , Bifidobacterium longum ) And Streptococcus thermophilus ( Streptococcus thermophilus ) It may be one or more lactic acid bacteria selected from.

According to one embodiment of the present invention, the lactic acid bacteria killed in step (2) are Lactobacillus plantarum , Lactobacillus rhamnosus , Bifidobacterium animalis lactis ( Bifidobacterium animalis ssp) lactis ), Bifidobacterium longum, and Streptococcus thermophilus.

According to one embodiment of the present invention, the first coating solution in step (2) is a mixture of red ginseng concentrate, enzyme, dead lactic acid bacteria and live lactic acid bacteria in a weight ratio of 100: 5-10: 1-3: 1-3 it could be

According to one embodiment of the present invention, between steps (2) and (3), (2-1) stirring the first coating solution at 20 to 30 ° C. for 1 to 20 hours at 10 to 50 rpm and fermenting; may be further performed.

According to one embodiment of the present invention, the fluidized bed coating granulator in the steps (3) and (5) has an intake air temperature of 60 to 90 ° C, a fluidized powder temperature of 40 to 50 ° C, and a spray air pressure for fluidization of 2.8 to 3.2 kg/cm 2 , and the input rate of the first coating solution or the second coating solution may be 1 to 200 g/min.

According to one embodiment of the present invention, the natural polysaccharide in step (4) is guar gum, xanthan gum, gum arabic, cellulose gum, tragacanth gum, rosy bean gum, karaya gum, ghatti gum, tara gum, and konjac gum It may be two or more types of gums selected from among.

According to one embodiment of the present invention, the natural polysaccharide may be a mixture of gellan gum and gum arabic in a weight ratio of 1:1-3.

According to one embodiment of the present invention, the double-coated red ginseng granules have a total of ginsenosides Rb1, Rg1, and Rg3 of at least 7.0 mg/g or more, a content of ginsenoside F2 of at least 5.0 mg/g or more, and ginsenoside F2 of at least 5.0 mg/g. The content of the senoside compound K may be at least 4.0 mg/g or more.

In addition, the present invention is a double-coated red ginseng granule prepared according to the above method, the total of ginsenosides Rb1, Rg1 and Rg3, ginsenoside F2 and ginsenoside compound K content is increased, and preservation and solubility are improved. to provide.

The double-coated red ginseng granules prepared according to the present invention have increased amounts of ginsenosides Rb1, Rg1 and Rg3, ginsenoside F2 and ginsenoside compound K, and improved preservation and enteric properties. In addition, the double-coated red ginseng granules have a high absorption rate of ginsenoside in the body, are easy to carry, and are easy to consume in a fixed amount, so they are very useful for improving the health of modern people.

Hereinafter, the present invention will be described in detail.

Throughout the specification, when a certain component is said to "include", it means that it may further include other components rather than excluding other components.

In the present specification, "ginsenoside index" can be expressed as ① the content of Compound K, or ② the sum of Rg1, Rb1, and Rg3 (Rg1+Rb1+Rg3), which indicates the production efficiency of Compound K and/or red ginseng granules can be an indicator of the quality of The red ginseng granules of the present invention in which the above indicators exceed a certain level have a high sum of Rg1, Rb1 and Rg3 and a high content of compound K, so the quality is very excellent.

The present invention comprises the steps of (1) preparing red ginseng concentrate seed powder by spray-drying red ginseng concentrate; (2) preparing a first coating solution by mixing red ginseng concentrate, enzyme, dead lactic acid bacteria, live lactic acid bacteria, and purified water; (3) After putting the red ginseng concentrate seed powder prepared in step (1) at the bottom of the fluidized bed coating granulator, inject heated air, and through a far-infrared ray irradiation device at the top of the fluidized bed coating granulator Wavelength in the range of 2 to 14 μm shaping into granules by spraying the primary coating solution of step (2) through a spray nozzle at the bottom of the fluidized bed coating granulator while irradiating far-infrared rays that emit; (4) preparing a secondary coating solution by mixing red ginseng concentrate, natural polysaccharide, and purified water; And (5) the secondary coating solution of step (4) is injected through the spray nozzle at the bottom of the fluidized bed coating granulator while introducing heated air after introducing the granules prepared in step (3) to the bottom of the fluidized bed coating granulator. It relates to a method for producing double-coated red ginseng granules with enhanced ginsenoside content and preservation, including the step of spraying and molding into double-coated granules.

In the following examples, the double-coated red ginseng granules prepared according to the present invention have higher ginsenoside indicators (sum of Rg1, Rb1, and Rg3, and higher content of ginsenoside compound K) than the control red ginseng granules prepared only with red ginseng concentrate. ) was excellent, had low hygroscopicity, and even when stored for a long time in a high-temperature, high-humidity environment, it was specifically confirmed that the reduction rate of the active ingredient content was low, and the preservability was improved. In addition, it was confirmed in detail that the double-coated red ginseng granules prepared according to the present invention are easy to drink, and the taste and smell are further improved because the solubility is further enhanced compared to the control red ginseng granules prepared only with red ginseng concentrate.

"Red ginseng (Panax ginseng C.A. Meyer)" used in the present invention means a product prepared by heating ginseng through steam or sun drying, preferably steam.

In the present specification, the term "ginseng" includes Panax ginseng, P. quiquefolius, P. notoginseng, P. japonicus, P. trifolium, and P. pseudoginseng), Vietnamese ginseng (P. vietnamensis) and American ginseng (Panax quinquefolium), but are not limited thereto. Preferably, the ginseng used in the present invention is Korean ginseng (Panax ginseng).

In addition, "ginseng" used in the present invention includes all ginseng processed in various forms, such as fresh ginseng, misam, white ginseng, taeguk ginseng, black ginseng, dehydrated ginseng, enzyme-treated ginseng, fermented ginseng, Sampi and ginseng gourd are excluded, and it is desirable that they are more than 4 years old.

The term "red ginseng concentrate" as used herein refers to a liquid obtained by concentrating red ginseng, using a commercially available one or a method known in the art, for example, a red ginseng juice obtained by extracting red ginseng, or obtained by extracting red ginseng. It can be prepared and used according to the method of concentrating red ginseng extract. At this time, it is preferable that the concentration of the red ginseng concentrate is 50 to 70 brix, preferably 60 to 70 brix.

As a preferred embodiment, the red ginseng concentrate is extracted by adding red ginseng to purified water in an amount of 7 to 20 times the weight of red ginseng and heating at 70 to 120 ° C, preferably 70 to 90 ° C, so that the volume of the first purified water is 5 to 50%. After that, it can be obtained by filtration and concentration.

In step (1) of the method for producing double-coated red ginseng granules of the present invention, red ginseng concentrate is spray-dried to prepare red ginseng concentrate seed powder.

When the red ginseng concentrate is powdered by spray drying in the process of drying, the yield of the powder is 30 to 40% by weight compared to the solid content of the red ginseng concentrate, which is significantly higher than the yield obtained at the laboratory level, which is suitable for mass production. .

As described above, the optimal conditions for spray drying the red ginseng concentrate were found to be conditions of an inlet temperature of 200 ± 10 ° C, an outlet temperature of 95 ± 5 ° C, and a spray pressure of 80 to 85 bar.

The red ginseng concentrate seed powder prepared in this way was obtained in a high yield of 85 to 90%, and was prepared with a uniform particle size.

The red ginseng concentrate may be irradiated with far-infrared rays emitting a wavelength in the range of 2 to 14 μm for 1 to 2 hours before or after spray drying. The red ginseng concentrate irradiated with far-infrared rays as described above is at least one selected from Rg1, Rd1, Rg3, Re, Rf, Rh1, Rh2, Rb2, Rb3, and Rd, preferably Rg1, Rd1, Rg3, Re, and Rf, compared to before far-infrared irradiation. The content of one or more ginsenosides selected from among increases. However, when the wavelength or time is out of the above range during the far-infrared irradiation process, the ginsenoside content may rather decrease.

In addition, in step (2) of the method for producing double-coated red ginseng granules of the present invention, a first coating solution is prepared by mixing red ginseng concentrate, enzyme, dead lactic acid bacteria, live lactic acid bacteria, and purified water.

The primary coating solution is a fermented red ginseng concentrate, which is subjected to complex fermentation using lactic acid bacteria and enzymes to change the composition of ginsenosides in the red ginseng concentrate through bioconversion. Specifically, the total of ginsenosides Rb1, Rg1 and Rg3, ginsenoside F2 and ginsenoside compound K content are increased in the composition of ginsenosides through the complex fermentation.

The enzyme is two or more complex enzymes selected from protease, alpha-glucosidase, beta-glucosidase, alpha-amylase, beta-amylase, glucoamylase, isoamylase, polygalacturonase, cellulase and hemicellulase It is preferable, and it is more preferable that it is a complex enzyme of protease and alpha-amylase. In addition, the protease and alpha-amylase are mixed in a weight ratio of 1: 1-4, preferably 1: 2-3, more preferably 1: 2.25-2.75. If the ratio of the complex enzyme is out of the above range, there is a concern that the enzyme activity may be inhibited or the enzyme may be deactivated.

The lactic acid bacteria are not particularly limited, but are preferably one or more lactic acid bacteria selected from the genus Lactobacillus, the genus Bifidobacterium and the genus Streptococcus, Lactobacillus plantarum , Lactobacillus rhamnosus ( Lactobacillus rhamnosus ), Bifidobacterium animalis ssp. lactis , Bifidobacterium longum ( Bifidobacterium longum ), and Streptococcus thermophilus ( Streptococcus thermophilus ). More preferably, it includes all strains of Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium lactis and Streptococcus thermophilus, more preferably Lactobacillus plantarum CBG-C21 (accession number: KACC92083P), Lactobacillus rhamnosus CBG-C14 (Accession number: KACC 91981P), Bifidobacterium Animalis lactis CBG-C10 (Accession number: KACC 15464P), Bifidobacterium longum CBG-C11 (Accession number: KTCT 11979P) and Stratococcus thermophilus CBG-C19 strain (accession number: KACC 91986P).

The lactic acid bacteria are 10 ⁶ to 10 ¹² CFU / ml, preferably 10 ⁸ to 10 ¹¹ CFU / ml, more preferably 10 ⁸ to 10 ¹⁰ CFU / ml, more preferably 10 ⁹ to 10 ¹⁰ CFU / ml It can be added concentrated.

In addition, the dead lactic acid bacteria body is not particularly limited, and any dead lactic acid bacteria body can be used, and may be prepared by killing lactic acid bacteria by heat treatment at a temperature of 80 ° C. or higher. Specifically, the lactic acid bacteria dead cells are Lactobacillus plantarum , Lactobacillus rhamnosus , Bifidobacterium lactis , Bifidobacterium longum , and Streptococcus It may be a dead cell body of one or more lactic acid bacteria selected from Streptococcus thermophilus . More preferably, it may be dead cells of lactic acid bacteria including all of Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium lactis and Streptococcus thermophilus strains, more preferably Lactobacillus plantarum CBG -C21 (Accession number: KACC92083P), Lactobacillus rhamnosus CBG-C14 (Accession number: KACC 91981P), Bifidobacterium Animalis lactis CBG-C10 (Accession number: KACC 15464P), Bifidobacterium longum CBG- It is a dead cell body of lactic acid bacteria including both C11 (accession number: KTCT 11979P) and Stratococcus thermophilus CBG-C19 strain (accession number: KACC 91986P). Further, the lactic acid bacteria dead cells preferably further include 10 to 30 parts by weight of Enterococcus faecalis dead cells based on 100 parts by weight of the lactic acid bacteria dead cells in terms of increasing the ginsenoside content.

The lactic acid bacteria dead cells are 10 ⁶ to 10 ¹² CFU / ml, preferably 10 ⁸ to 10 ¹¹ CFU / ml, more preferably 10 ⁸ to 10 ¹⁰ CFU / ml, more preferably 10 ⁹ to 10 ¹⁰ CFU / It can be added concentrated to ml.

The primary coating solution may be a mixture of red ginseng concentrate, enzyme, dead lactic acid bacteria and live lactic acid bacteria in a weight ratio of 100: 5-10: 1-3: 1-3. When the weight ratio of red ginseng concentrate, enzyme, dead lactic acid bacteria, and live lactic acid bacteria is within the above range, the bioconversion rate of converting the high molecular weight ginsenoside contained in the red ginseng concentrate into low molecular weight ginsenoside with high digestion and absorption rate in the human body increases to the maximum. In addition, it shows an excellent effect in terms of sensory. If the weight ratio of the red ginseng concentrate, enzyme, dead lactic acid bacteria, and live lactic acid bacteria is less than the lower limit, the bioconversion rate to low molecular weight ginsenoside may be significantly lowered, and if it exceeds the upper limit, abnormal fermentation occurs, There may be off-flavor and taste, and the bioconversion rate and the growth rate of lactic acid bacteria may rather be lowered.

After mixing the red ginseng concentrate, enzyme, dead lactic acid bacteria and live lactic acid bacteria, purified water may be added to adjust the concentration of the mixed solution to 10-30 brix, preferably 16-24 brix.

On the other hand, between steps (2) and (3), (2-1) the primary coating solution is stirred at 20 to 30 ° C. for 1 to 20 hours, preferably at 10 to 50 rpm for 5 to 15 hours. and fermenting; may be further performed.

In addition, in step (3) of the method for producing double-coated red ginseng granules of the present invention, the red ginseng concentrate seed powder prepared in step (1) is put at the bottom of the fluidized bed coating granulator, and the far-infrared ray irradiation device at the top of the fluidized bed coating granulator While irradiating far-infrared rays emitting wavelengths in the range of 2 to 14 μm through a spray nozzle at the bottom of the fluidized bed coating granulator, the first coating solution of step (2) is sprayed to form granules.

As described above, the red ginseng granules prepared while irradiating far-infrared rays emitting wavelengths in the range of 2 to 14 μm in the fluidized bed coating granulator were compared to before irradiation with far-infrared rays, Rg1, Rd1, Rg3, Re, Rf, Rh1, Rh2, Rb2, Rb3 and The content of at least one ginsenoside selected from Rd, preferably at least one selected from Rg1, Rd1, Rg3, Re and Rf is further increased. However, when the wavelength is out of the above range during the far-infrared irradiation process, the ginsenoside content may rather decrease.

The fluidized bed coating granulator has an intake air temperature of 60 to 90 ° C, a fluidization powder temperature of 40 to 50 ° C, a spray air pressure for fluidization of 2.8 to 3.2 kg / cm 2, and a first coating solution or a second coating solution is introduced. The rate may be 1 to 200 g/min, preferably 80 to 120 g/min. The operating time of the fluidized bed coating granulator may vary depending on the input amount of raw materials, but since it takes 1 to 20 hours, preferably 5 to 15 hours, the first coating solution loaded into the fluidized bed coating granulator is loaded into the fluidized bed coating granulator. In this state, the red ginseng concentrate contained in the primary coating solution undergoes complex fermentation due to enzymes, dead lactic acid bacteria, and live lactic acid bacteria, and bioconversion of the polymer ginsenosides contained in the red ginseng concentrate occurs.

When the red ginseng concentrate seed powder is granulated under the above conditions, the granules are well granulated, the properties of the granules are uniform, the solubility of the granules is improved, the yield of the granules is high, and the preservation of the granules is increased. Above all, when granulation is performed under the above conditions, the content of low molecular weight ginsenosides such as ginsenoside compound K increases, and the ginsenoside index becomes more excellent.

In step (4) of the method for producing double-coated red ginseng granules of the present invention, a secondary coating solution is prepared by mixing red ginseng concentrate, natural polysaccharide, and purified water.

The natural polysaccharide may be at least two gums selected from guar gum, xanthan gum, gum arabic, cellulose gum, tragacanth gum, rogers bean gum, karaya gum, ghatti gum, tara gum, and konjac gum. Preferably, the natural polysaccharide may be gellan gum and gum arabic, more preferably a mixture of gellan gum and gum arabic in a weight ratio of 1:1-3, more preferably 1:1.5-2.5. there is. When the weight ratio of gellan gum and gum arabic is within the above range, the yield of granules is remarkably increased, the preservation of red ginseng granules is markedly increased, and excellent sensory effects are exhibited. When the weight ratio of gum arabic to gellan gum is less than the lower limit, the effect of increasing the preservation of red ginseng granules is insignificant, and when it exceeds the upper limit, the yield of granules may be rather low.

The natural polysaccharide may be mixed with water to form an aqueous solution, and may be mixed in an amount of 1 to 10 parts by weight, preferably 3 to 7 parts by weight, in dry weight, based on 100 parts by weight of the red ginseng concentrate.

After mixing the red ginseng concentrate and the natural polysaccharide, purified water may be added to adjust the concentration of the mixed solution to 10-30 brix, preferably 16-24 brix.

In step (5) of the method for producing double-coated red ginseng granules of the present invention, the granules prepared in step (3) are injected into the bottom of the fluidized bed coating granulator, and heated air is introduced into the spray nozzle at the bottom of the fluidized bed coating granulator. The second coating solution of step (4) is sprayed through and molded into double-coated granules.

The fluidized bed coating granulator has an intake air temperature of 60 to 90 ° C, a fluidization powder temperature of 40 to 50 ° C, a spray air pressure for fluidization of 2.8 to 3.2 kg / cm 2, and a first coating solution or a second coating solution is introduced. The rate may be 1 to 200 g/min, preferably 80 to 120 g/min.

When the granules prepared in step (3) are granulated into double-coated granules under the above conditions, the granules are well granulated, the properties of the granules become uniform, the solubility of the granules is improved, the yield of the granules is high, and the preservation of the granules is improved. It rises even higher.

The double-coated red ginseng granules prepared according to the present invention include red ginseng concentrate seed powder, a primary coating layer in which ginsenoside index in red ginseng concentrate is further improved by complex fermentation of red ginseng concentrate with enzymes, lactic acid bacteria and dead lactic acid bacteria, and red ginseng concentrate and red ginseng concentrate. It is composed of a secondary coating layer composed of a mixed solution of natural polysaccharides, and the total amount of ginsenosides Rb1, Rg1 and Rg3, the content of ginsenoside F2 and ginsenoside compound K is increased, and preservation and solubility are improved.

Specifically, in the double-coated red ginseng granules according to the present invention, the sum of ginsenosides Rb1, Rg1 and Rg3 is at least 7.0 mg/g or more, preferably 7.0 to 15.0 mg/g, more preferably 9.0 to 13.0 mg/g. The content of ginsenoside F2 is at least 5.0 mg/g or more, preferably 5.0 to 10.0 mg/g, more preferably 6.0 to 8.0 mg/g, and the content of ginsenoside compound K is at least 4.0 mg/g. g or more, preferably 4.0 to 7.0 mg/g, more preferably 4.5 to 6.5 mg/g.

In addition, the present invention relates to double-coated red ginseng granules prepared according to the above method, in which the total amount of ginsenosides Rb1, Rg1 and Rg3, ginsenoside F2 and ginsenoside compound K content are increased, and preservation and solubility are improved. will be.

Regarding the content of active ingredients in the red ginseng granules, look at the red ginseng item and indicator component content of "Standards and Specifications for Health Functional Foods" (MFDS Notification No. 2016-143, 2016.12.21.), "Ginsenoside Rg1 , 2.5-34 mg/g of Rb1 and Rg3" is described as the standard value. In addition, if you look at the "requirements of the final product" of this document, functionally, it meets the requirements of "enhancement of immunity, improvement of fatigue, improvement of blood flow/memory through inhibition of platelet aggregation, antioxidant, and can help menopausal women's health" In addition, looking at the details of the document, in order to help "enhance immunity and improve fatigue", the content of the index component (Rg1 + Rb1 + Rg3) is 3-80 mg/g, "platelet aggregation" It is written that the sum of the index components should be 2.4-80 mg/g to help "improvement of blood flow/memory through inhibition, and antioxidant".

As described in the following examples, the double-coated red ginseng granules prepared according to the present invention have a compound K content of at least 4.0 mg/g or more and the sum of the index components (Rg1, Rb1, and Rg3) at least 7.0 mg/g. Therefore, it has a very good ginsenoside composition that can help improve immunity, improve fatigue, improve blood flow, improve memory, and antioxidant.

Hereinafter, the present invention will be described in detail by examples, but the present invention is not limited by the following examples.

<Example>

Preparation Example 1: Preparation of fermented red ginseng concentrate (first coating solution)

(a) Red ginseng (processed 4-year-old ginseng) was added to purified water in an amount 10 times the weight of red ginseng, heated to 90 ° C, extracted to make 20% of the volume of the initial purified water, and then filtered with filter paper to obtain a red ginseng extract with a concentration of 60 brix. After concentrating as much as possible, sterilization was performed at 121 ° C. for 15 minutes to obtain a red ginseng concentrate.

(b) Lactobacillus plantarum CBG-C21 (accession number: KACC92083P), Lactobacillus rhamnosus CBG-C14 (accession number: KACC 91981P), Bifidobacterium animalis lactis CBG-C10 (accession number: KACC 91981P) in 10 mL of MRS medium Accession number: KACC 15464P), Bifidobacterium longum CBG-C11 (accession number: KTCT 11979P), and Stratococcus thermophilus CBG-C19 strain (accession number: KACC 91986P) 5 kinds of lactic acid strains were inoculated in the same amount. After mixing and fermentation at 37 ℃ for 48 hours to obtain a lactic acid bacteria pre-culture (1 × 10 ⁹ CFU / ㎖).

(c) A portion of the lactic acid bacteria pre-culture was centrifuged at 3000 rpm for 10 minutes to remove the medium of the supernatant. Here, 0.75% physiological saline was added to suspend the lactic acid bacteria strain, and then centrifuged at 3000 rpm for 10 minutes to remove the saline. This method was repeated three times to completely remove medium components, resuspend at a concentration of 1×10 ⁹ CFU/ml, and then heat at 95° C. for 30 minutes to obtain dead lactic acid bacteria.

(d) Red ginseng concentrate prepared in step (a), enzyme (protease: alpha-amylase = 1: 2.5 (w/w)), lactic acid bacteria pre-culture solution prepared above, and killed lactic acid bacteria cells prepared above 100: 7: 1.5: 1.5 were mixed in a weight ratio of In addition, purified water was added to the mixture to adjust the concentration to 20 brix to prepare a fermented red ginseng concentrate (first coating solution).

(e) The fermented red ginseng concentrate (first coating solution) was fermented at 26 ° C. for 10 hours while stirring at 30 rpm.

Preparation Example 2: Preparation of fermented red ginseng concentrate (first coating solution) - Addition of dead cells of Enterococcus faecalis

It was carried out in the same manner as in Preparation Example 1, but in step (c), 20 parts by weight of dead Enterococcus faecalis was further added to 100 parts by weight of dead lactic acid bacteria of Preparation Example 1 to obtain dead lactic acid bacteria.

The Enterococcus faecalis dead cells were obtained by applying the method of steps (b) and (c) of Preparation Example 1.

Preparation Example 3: Preparation of fermented red ginseng concentrate (first coating solution) - using a single enzyme (alpha-amylase)

It was carried out in the same manner as in Preparation Example 1, but in step (d), a fermented red ginseng concentrate was prepared using a single enzyme (alpha-amylase) instead of a complex enzyme.

Preparation Example 4: Preparation of fermented red ginseng concentrate (first coating solution) - using a single enzyme (protease)

It was carried out in the same manner as in Preparation Example 1, but in step (d), a fermented red ginseng concentrate was prepared using a single enzyme (protease) instead of a complex enzyme.

Preparation Example 5: Preparation of fermented red ginseng concentrate (primary coating solution) - using a single lactic acid bacteria (Lactobacillus plantarum)

It is carried out in the same manner as in Preparation Example 1, but instead of inoculating 5 types of lactic acid bacteria in step (b), only Lactobacillus plantarum CBG-C21 (accession number: KACC92083P) is obtained by inoculating only red ginseng concentrate fermented product using a pre-culture of lactic acid bacteria. was manufactured.

Preparation Example 6: Preparation of fermented red ginseng concentrate (primary coating solution) - using a single lactic acid bacteria (Lactobacillus rhamnosus)

It is carried out in the same manner as in Preparation Example 1, but instead of inoculating 5 types of lactic acid bacteria in step (b), only Lactobacillus rhamnosus CBG-C14 (accession number: KACC 91981P) is obtained. was manufactured.

Preparation Example 7: Preparation of fermented red ginseng concentrate (first coating solution) - using a single lactic acid bacteria (Bifidobacterium animalis lactis)

It was carried out in the same manner as in Preparation Example 1, but instead of inoculating 5 types of lactic acid bacteria in step (b), using a pre-culture of lactic acid bacteria obtained by inoculating only Bifidobacterium Animalis lactis CBG-C10 (accession number: KACC 15464P) A fermented product of red ginseng concentrate was prepared.

Preparation Example 8: Preparation of fermented red ginseng concentrate (first coating solution) - using a single lactic acid bacteria (Bifidobacterium longum)

It was carried out in the same manner as in Preparation Example 1, but instead of inoculating 5 types of lactic acid bacteria in step (b), only Bifidobacterium longum CBG-C11 (accession number: KTCT 11979P) was inoculated and fermented red ginseng concentrate using the pre-cultured lactic acid bacteria obtained. water was prepared.

Preparation Example 9: Preparation of fermented red ginseng concentrate (primary coating solution) - using a single lactic acid bacteria (Streptococcus thermophilus)

It was carried out in the same manner as in Preparation Example 1, but instead of inoculating 5 types of lactic acid bacteria in step (b), red ginseng A concentrate fermented product was prepared.

Comparative Preparation Example 1: Red ginseng concentrate only

A red ginseng concentrate was obtained in the same manner as in step (a) of Preparation Example 1.

Comparative Preparation Example 2: Preparation of fermented red ginseng concentrate (first coating solution) - enzyme omitted

It was carried out in the same manner as in Preparation Example 1, but the enzyme was omitted in step (d) and a fermented red ginseng concentrate was prepared.

Comparative Preparation Example 3: Preparation of fermented red ginseng concentrate (primary coating solution) - lactic acid bacteria pre-culture solution omitted

It was carried out in the same manner as in Preparation Example 1, but the lactic acid bacteria pre-culture solution was omitted in step (d) and a fermented product of red ginseng concentrate was prepared.

Comparative Preparation Example 4: Preparation of fermented red ginseng concentrate (primary coating solution) - Omit dead cells of lactic acid bacteria

It was carried out in the same manner as in Preparation Example 1, but in step (d), the dead cells of lactic acid bacteria were omitted, and a fermented product of red ginseng concentrate was prepared.

Preliminary test example: measurement of ginsenoside content

Analytical reagents were water (HPLC grade, JT Baker, USA) and acetonitrile (HPLC grade, acetonitrile; JT Baker), and standard products were ginsenosides Rg1, Rg3, Rb1 (HWI ANALYTIK GmbH, Germany); F2 and Compound K were used.

HPLC-UVD (High Performance Liquid Chromatography-Ultraviolet Detector) used Ultimate 3000 series (Thermo, USA).

About 2 g of samples of Preparation Example and Comparative Preparation Example were taken in a 50 ml flask, filled with distilled water up to the mark, completely dissolved, and filtered through a syringe filter (0.45 μm).

Ginsenoside was analyzed by high performance liquid chromatography (HPLC). A Capcell pak C18 column (Shiseido, 5 μm, 250 × 4.6 mm) was used as the column, and acetonitrile and tertiary distilled water were used as the mobile phase. The sample injection amount was 10 μl, the flow rate was 1.0 ml/min, the temperature was 30° C., and the UV detector was measured at 203 nm under the mobile phase conditions shown in Table 1 below.

time (minutes) A: water B: Acetonitrile 0 80 20 5 80 20 20 77 23 25 70 30 30 60 40 35 50 50 60 15 85 65 15 85 66 80 20 75 80 20

The quantitative value of the sample was calculated by the following formula after substituting the peak area of the measured value into the calibration curve (y = ax + b) formula, and is shown in Table 2 below. In addition, the calibration curve was calculated by the least squares method by applying the concentration on the x-axis and the peak area on the y-axis.

Quantitative value (mg/g) = C x (a x b)/S x 1/1,000

(C: Concentration of individual ginsenosides in the test solution (μg/ml),

a: total amount of test solution (ml), b: dilution factor

S: sampling amount (g), 1/1,000: unit conversion factor)

division Ginsenoside content (mg/g) Rg1+Rb1+Rg3 Re Rf F2 compound K Preparation Example 1 10.44±0.23 3.12±0.11 1.12±0.05 6.13±0.15 4.77±0.19 Preparation Example 2 10.26±0.64 3.27±0.07 1.17±0.13 6.29±0.26 5.14±0.32 Preparation Example 3 10.13±0.19 2.56±0.06 0.63±0.03 4.12±0.06 3.26±0.18 Production Example 4 10.05±0.08 2.51±0.07 0.57±0.02 3.59±0.09 3.02±0.25 Preparation Example 5 9.64±0.17 2.95±0.16 0.78±0.05 4.64±0.21 3.16±0.14 Preparation Example 6 9.56±0.41 2.87±0.15 0.65±0.02 4.41±0.22 3.05±0.07 Preparation Example 7 9.13±0.38 2.79±0.24 0.59±0.03 4.07±0.15 2.96±0.19 Preparation Example 8 9.06±0.24 2.73±0.18 0.57±0.05 2.16±0.13 2.69±0.21 Preparation Example 9 9.27±0.13 2.64±0.09 0.60±0.06 3.05±0.06 2.57±0.08 Comparative Preparation Example 1 8.17±0.23 2.41±0.07 0.40±0.03 N.D. N.D. Comparative Preparation Example 2 9.96±0.31 2.84±0.18 0.96±0.05 1.03±0.10 2.13±0.06 Comparative Preparation Example 3 8.23±0.16 2.59±0.25 0.54±0.04 7.24±0.07 1.67±0.12 Comparative Preparation Example 4 8.02±0.11 2.67±0.14 0.83±0.02 0.92±0.03 2.06±0.07

Looking at Table 2, the fermented product of red ginseng concentrate according to Preparation Example has a higher total of ginsenosides Rg1 + Rb1 + Rg3 and a higher content of ginsenoside F2 and compound K than Comparative Preparation Example. It can be confirmed that it is more excellent and the bioavailability is higher.

Example 1: Preparation of double-coated red ginseng granules

(1) The red ginseng concentrate prepared in step (a) of Preparation Example 1 was irradiated with far infrared rays having a wavelength of 10 μm for 90 minutes using a far infrared dryer (Korea Energy Technology, Seoul, Korea).

After putting the red ginseng concentrate into a spray dryer under the conditions of an inlet temperature of 200 ± 10 ° C, an outlet temperature of 95 ± 5 ° C, a chamber pressure of -4 mmHg, and a spray pressure of 80 to 85 bar Red ginseng concentrate seed powder was obtained by spray drying.

(2) The first coating solution was prepared in the same manner as (b), (c) and (d) of Preparation Example 1.

(3) After putting the red ginseng concentrate seed powder prepared in step (1) at the bottom of the fluidized bed coating granulator, heated air was introduced, and far-infrared rays of 10 μm wavelength were irradiated through the far-infrared ray irradiation device at the top of the fluidized bed coating granulator While doing so, the first coating solution of step (2) was sprayed through the spray nozzle at the bottom of the fluidized bed coating granulator to form granules. At this time, the fluidized bed coating granulator has an intake air temperature of 80 ° C, a red ginseng concentrate seed powder temperature of 45 ° C, a spray air pressure for fluidization of 3.0 kg / cm 2, and an input rate of the first coating solution of 90 g / min. set up

(4) A natural polysaccharide aqueous solution was prepared by mixing gellan gum and gum arabic in a weight ratio of 1:2 and then adding purified water.

After mixing 5 parts by weight of the dry weight of the natural polysaccharide aqueous solution with 100 parts by weight of the red ginseng concentrate prepared in step (a) of Preparation Example 1, the concentration of the mixed solution was adjusted to 20 brix to prepare a secondary coating solution. .

(5) After putting the granules prepared in step (3) at the bottom of the fluidized bed coating granulator, while introducing heated air, spray the secondary coating solution of step (4) through the spray nozzle at the bottom of the fluidized bed coating granulator. and molded into double-coated granules. At this time, the fluidized bed coating granulator had an intake air temperature of 80 ° C, a granule temperature of 45 ° C, a spraying air pressure for fluidization of 3.0 kg / cm 2, and an input rate of the secondary coating solution at 90 g / min. .

Example 2: Preparation of double-coated red ginseng granules - addition of fermentation process

It is carried out in the same manner as in Example 1, but between steps (2) and (3), (2-1) fermenting the first coating solution at 25 ° C. for 10 hours with stirring at 25 rpm; As a result, double-coated red ginseng granules were prepared.

Examples 3 to 10: Preparation of double-coated red ginseng granules

It was carried out in the same manner as in Example 1, but in the process of preparing the first coating solution in step (2), double-coated red ginseng granules were prepared by applying the methods of Preparation Examples 2 to 9 instead of Preparation Example 1, respectively.

division Example of application when preparing a primary coating solution Example 3 Preparation Example 2 Example 4 Preparation Example 3 Example 5 Production Example 4 Example 6 Preparation Example 5 Example 7 Preparation Example 6 Example 8 Preparation Example 7 Example 9 Preparation Example 8 Example 10 Preparation Example 9

Example 11: Preparation of double-coated red ginseng granules - gum arabic only

In the same manner as in Example 1, in step (4), an aqueous solution of natural polysaccharide was prepared using only gum arabic instead of gellan gum and gum arabic, and then red ginseng granules were prepared.

Example 12: Preparation of double-coated red ginseng granules - gellan gum only

In the same manner as in Example 1, in step (4), a natural polysaccharide aqueous solution was prepared using only gellan gum instead of gellan gum and gum arabic, and then red ginseng granules were prepared.

Example 13: Preparation of double-coated red ginseng granules - Omission of far-infrared radiation in step (1)

It was carried out in the same manner as in Example 1, except for the far-infrared irradiation in step (1), and double-coated red ginseng granules were prepared.

Control: Preparation of red ginseng granules

(1) Red ginseng concentrate seed powder was prepared in the same manner as in step (1) of Example 1.

(2) Red ginseng granules were prepared in the same manner as in step (2) of Example 1, but red ginseng granules were prepared using red ginseng concentrate instead of the first coating solution.

Comparative Example 1: Preparation of red ginseng granules - far-infrared radiation omitted

Red ginseng granules were prepared in the same manner as in Example 1, except that far-infrared radiation was omitted in both steps (1) and (3).

Comparative Example 2: Preparation of red ginseng granules - 1st coating omitted

Red ginseng granules were prepared in the same manner as in Example 1, except that the primary coating process of steps (2) and (3) was omitted.

Comparative Example 3: Preparation of red ginseng granules - 2nd coating omitted

Red ginseng granules were prepared in the same manner as in Example 1, except that the secondary coating process of steps (4) and (5) was omitted.

Comparison 4: Preparation of red ginseng granules - omitting enzymes

It was carried out in the same manner as in Example 1, but in the process of preparing the first coating solution in step (2), the method of Comparative Preparation Example 2 was applied instead of Preparation Example 1 to prepare double-coated red ginseng granules.

Comparative Example 5: Preparation of red ginseng granules - lactic acid bacteria pre-culture solution omitted

It was carried out in the same manner as in Example 1, but in the process of preparing the first coating solution in step (2), the method of Comparative Preparation Example 3 was applied instead of Preparation Example 1 to prepare double-coated red ginseng granules.

Comparative Example 6: Preparation of red ginseng granules - Omit dead cells of lactic acid bacteria

It was carried out in the same manner as Example 1, but in the process of preparing the first coating solution in step (2), the method of Comparative Preparation Example 4 was applied instead of Preparation Example 1 to prepare double-coated red ginseng granules.

< Test Example>

statistical processing

All statistical analyzes were performed using SPSS version 18.0 (IBM, Chicago, IL, USA). A one-way ANOVA with Duncan's post-hoc test was used to determine the difference (p <0.05) in the mean value between the experimental groups. Each of the following experiments was repeated at least three times, and each experimental data was expressed as mean ± standard deviation (SD).

Test Example 1: Measurement of ginsenoside content of red ginseng granules

The ginsenoside content of the red ginseng granules according to the above Examples and Comparative Examples was measured in the same manner as in the preliminary test examples and is shown in Table 4 below.

division Ginsenoside content (mg/g) Rg1+Rb1+Rg3 Re Rf F2 compound K control group 11.12±0.17 3.23±0.14 0.38±0.03 N.D. N.D. Example 1 11.49±0.33 4.03±0.26 1.25±0.05 5.52±0.25 4.78±0.23 Example 2 11.15±0.07 4.18±0.30 1.31±0.09 5.60±0.21 5.12±0.21 Example 3 11.26±0.15 3.93±0.23 1.32±0.12 5.64±0.43 4.97±0.09 Example 4 11.18±0.13 3.90±0.21 1.16±0.10 3.51±0.31 2.86±0.05 Example 5 11.14±0.09 3.67±0.17 1.13±0.04 3.04±0.18 2.79±0.14 Example 6 10.86±0.18 3.61±0.13 0.84±0.03 3.96±0.23 4.37±0.20 Example 7 10.64±0.29 3.54±0.28 0.80±0.06 3.77±0.13 4.30±0.09 Example 8 10.59±0.35 3.48±0.23 0.77±0.02 3.55±0.29 4.24±0.13 Example 9 10.60±0.30 3.57±0.11 0.73±0.03 1.69±0.15 4.20±0.17 Example 10 10.53±0.17 3.60±0.05 0.75±0.04 2.51±0.10 4.21±0.09 Example 11 10.84±0.14 3.87±0.07 1.18±0.05 5.39±0.06 4.73±0.28 Example 12 11.20±0.10 3.57±0.10 1.12±0.05 5.31±0.15 4.70±0.23 Example 13 10.52±0.05 3.67±0.14 1.17±0.01 5.37±0.33 4.22±0.16 Comparative Example 1 9.49±0.33 3.03±0.26 1.05±0.04 5.02±0.23 3.79±0.21 Comparative Example 2 10.45±0.07 3.34±0.18 0.63±0.03 1.26±0.09 N.D. Comparative Example 3 11.34±0.18 3.89±0.09 1.16±0.07 5.09±0.13 4.65±0.19 Comparative Example 4 11.06±0.08 3.77±0.26 1.09±0.08 2.95±0.11 5.59±0.08 Comparative Example 5 10.32±0.19 3.96±0.20 1.14±0.05 5.29±0.14 1.38±0.04 Comparative Example 6 11.12±0.21 3.37±0.18 0.71±0.03 1.56±0.12 4.02±0.18

As shown in Table 4, the red ginseng granules according to the examples have a higher total of ginsenosides Rg1 + Rb1 + Rg3 and a higher content of ginsenoside F2 and compound K than those of the comparative examples. It can be confirmed that it is more excellent and the bioavailability is higher.

Test Example 2: Measurement of enteric properties of red ginseng granules - solubility in acidic solution

Put 100 g of water at 25 ° C in a transparent container, adjust the pH to 3.0 with 1N hydrochloric acid solution, put 5 g of each red ginseng granule according to Examples and Comparative Examples, close the lid, and place it on an up-and-down vibrator to 170 The time until the red ginseng granules were completely dissolved was measured under the conditions of rpm and vertical vibration width of 7 cm. The measurement results are shown in Table 5 below.

division Time required for complete dissolution (seconds) control group 26.4 Example 1 41.2 Example 2 48.6 Example 3 49.5 Example 4 43.2 Example 5 48.3 Example 6 43.2 Example 7 48.6 Example 8 42.1 Example 9 48.3 Example 10 42.1 Example 11 41.3 Example 12 40.4 Example 13 41.1 Comparative Example 1 41.2 Comparative Example 2 32.2 Comparative Example 3 29.5

As shown in Table 5, it was confirmed that the double-coated red ginseng granules according to the examples showed an enteric effect by increasing the dissolution time in acidic solution conditions such as gastric juice by double coating. That is, it is believed that the double-coated red ginseng granules according to the present invention can safely pass through an acidic stomach and maintain the activity of enzymes and lactic acid bacteria up to the intestine when ingested.

Test Example 3: Measurement of hygroscopicity of red ginseng granules

In general, it is known that the hygroscopicity of granules is closely related to storage stability, and when the hygroscopicity is high, lumps and hardening are likely to occur and storage stability is low.

200 g of red ginseng granules according to Examples and Comparative Examples were placed in a 500 mL beaker, stored for 7 days using a humidity chamber adjusted to a temperature of 40 ° C and a relative humidity of 80%, and the degree of weight increase was measured as follows. Table 6 shows.

division weight (g) 0 days 3 days later 7 days later control group 200 21.13 22.09 Example 1 200 20.10 20.12 Example 2 200 20.09 20.10 Example 3 200 20.10 20.12 Example 4 200 21.05 21.98 Example 5 200 21.00 21.93 Example 6 200 20.86 21.89 Example 11 200 20.54 21.06 Example 12 200 20.68 21.37 Example 13 200 20.10 20.11 Comparative Example 1 200 20.10 20.13 Comparative Example 2 200 20.16 20.57 Comparative Example 3 200 22.91 25.83

As shown in Table 6, it can be seen that the red ginseng granules according to the examples have relatively low hygroscopicity compared to the comparative example without double coating.

Test Example 4: Accelerated test stability test - active ingredient safety evaluation

The red ginseng granules according to the above Examples and Comparative Examples were stored at 40 ° C and 70% relative humidity for 40 days, and the ginsenoside content and the number of lactic acid bacteria were measured, and the content of active ingredients (ginsenoside content and The number of lactic acid bacteria) was calculated as a relative ratio to the active ingredient content on the initial storage day (day 0) and is shown in Table 7 below.

division Total ginsenoside content
(% of 0 days) Lactobacillus survival rate (%) control group 0 days 100 - 20 days 96.12 - 40 days 90.67 - Example 1 0 days 100 100 20 days 99.80 85.4 40 days 99.62 68.5 Example 2 0 days 100 100 20 days 99.93 94.7 40 days 99.80 75.6 Example 3 0 days 100 100 20 days 99.85 87.3 40 days 99.66 70.3 Example 4 0 days 100 100 20 days 98.91 83.6 40 days 97.66 63.4 Example 5 0 days 100 100 20 days 98.59 82.4 40 days 97.25 61.7 Example 6 0 days 100 100 20 days 96.91 86.6 40 days 92.43 59.4 Example 11 0 days 100 100 20 days 97.39 83.9 40 days 94.58 58.3 Example 12 0 days 100 100 20 days 96.53 77.4 40 days 93.09 53.6 Comparative Example 1 0 days 100 100 20 days 99.78 83.4 40 days 99.59 66.5 Comparative Example 2 0 days 100 100 20 days 95.87 81.9 40 days 91.63 60.5 Comparative Example 3 0 days 100 100 20 days 95.16 76.3 40 days 91.54 51.6 Comparative Example 4 0 days 100 100 20 days 94.05 85.2 40 days 91.68 62.7 Comparative Example 5 0 days 100 - 20 days 94.23 - 40 days 91.86 - Comparative Example 6 0 days 100 100 20 days 96.31 85.4 40 days 95.54 62.5

As shown in Table 7, the double-coated red ginseng granules according to the embodiment of the present invention showed a small decrease in the total ginsenoside content and the number of viable lactic acid bacteria over the storage period in a high temperature and high humidity environment compared to the control group consisting only of red ginseng concentrate. Able to know.

Test Example 5: Antioxidation test

After dissolving red ginseng granules according to Examples and Comparative Examples in distilled water, test solutions diluted in various concentrations were prepared.

100 μL of the test solution diluted in various concentrations and 100 μL of 0.15 mM DPPH (2,2-diphenyl-1-picrylhydrazyl) solution were added to a 96-well plate, mixed, reacted at room temperature for 30 minutes, and absorbance was measured at a wavelength of 570 nm. . The resulting value showed the free radical removal activity compared to the control group to which no sample was added, and ascorbic acid was used as a positive control group. The DPPH radical scavenging activity was calculated by the formula below, and the SC ₅₀ value was shown in Table 8 below by calculating the concentration at which the radical scavenging activity was 50% in the calibration curve for the radical scavenging activity for each concentration.

[Equation 1]

DPPH radical scavenging activity (%) = (1-A/B) x 100

(A: absorbance of sample-added area, B: absorbance of untreated area)

division SC ₅₀ (mg/mL) control group 1.20±0.02 Example 1 1.03±0.05 Example 2 1.00±0.03 Example 3 1.03±0.01 Example 11 1.07±0.10 Example 12 1.17±0.06 Example 13 1.06±0.02 Comparative Example 1 1.08±0.01 Comparative Example 2 1.22±0.03 Comparative Example 3 1.16±0.02

As shown in Table 8, it can be confirmed that the double-coated red ginseng granules of the present invention maintain their antioxidant capacity at the level of the existing red ginseng concentrate even though the composition of saponin is changed compared to the control group consisting only of the red ginseng concentrate.

Test Example 6: Sensory evaluation

Sensory evaluation of the red ginseng granules according to the above Examples and Comparative Examples was performed.

Specifically, for sensory evaluation, taste, smell, and overall acceptability were evaluated when 1.5 g of red ginseng granules were dissolved in 100 mL of water and consumed.

A panel of 50 men and women with more than 3 years of experience in sensory testing in the food-related field was formed, and the preference test was conducted according to the 9-point scale method (minimum 1 point, maximum 9 points), and statistical processing was performed using the SPSS 12.0 statistical program. was used and significance was verified at the significance level p<0.05 by a multiple range test (DMR-test; Duncan multiple range test). The above evaluation results are shown in Table 9 below.

division taste smell overall sign control group 5.8 5.7 5.8 Example 1 7.3 6.9 7.1 Example 2 7.7 7.3 7.5 Example 3 7.3 7.0 7.2 Example 4 6.7 6.6 6.6 Example 5 6.6 6.5 6.5 Example 6 6.7 6.5 6.6 Example 7 6.5 6.3 6.4 Example 8 6.0 5.9 5.9 Example 9 6.1 6.3 6.2 Example 10 5.9 5.9 5.8 Example 11 7.1 6.6 6.7 Example 12 6.9 6.5 6.7 Example 13 7.2 6.8 7.0 Comparative Example 1 7.2 6.8 7.0 Comparative Example 2 6.2 6.1 6.1 Comparative Example 3 6.9 6.8 6.8 Comparative Example 4 7.0 6.7 6.9 Comparative Example 5 6.5 6.2 6.3 Comparative Example 6 7.1 6.5 6.8

Looking at Table 9, it was confirmed that the red ginseng granules of Example had better overall acceptability than those of Comparative Example.

Although the present invention has been described in terms of the preferred embodiments mentioned above, various modifications and variations are possible without departing from the spirit and scope of the invention. The appended claims also cover such modifications and variations as fall within the subject matter of this invention.

Claims (13)

(1) preparing red ginseng concentrate seed powder by spray-drying red ginseng concentrate;
(2) preparing a first coating solution by mixing red ginseng concentrate, protease and alpha-amylase at a weight ratio of 1: 1-4, lactic acid bacteria, dead lactic acid bacteria, and purified water;
(2-1) fermenting the primary coating solution;
(3) After putting the red ginseng concentrate seed powder prepared in step (1) at the bottom of the fluidized bed coating granulator, inject heated air, and through a far-infrared ray irradiation device at the top of the fluidized bed coating granulator Wavelength in the range of 2 to 14 μm shaping into granules by spraying the primary coating solution fermented in step (2-1) through a spray nozzle at the bottom of the fluidized bed coating granulator while irradiating far-infrared rays that emit;
(4) preparing a secondary coating solution by mixing red ginseng concentrate, natural polysaccharide, and purified water; and
(5) After putting the granules prepared in step (3) at the bottom of the fluidized bed coating granulator, while introducing heated air, spray the secondary coating solution of step (4) through the spray nozzle at the bottom of the fluidized bed coating granulator. Method for producing double-coated red ginseng granules with increased preservation of the total amount of ginsenosides Rb1, Rg1 and Rg3, ginsenoside F2 and ginsenoside compound K content, including the step of molding into double-coated granules. . According to claim 1,
Method for producing double-coated red ginseng granules, characterized in that the red ginseng concentrate in step (1) is irradiated with far-infrared rays emitting wavelengths in the range of 2 to 14 μm before or after spray drying. delete According to claim 1,
The method for producing double-coated red ginseng granules, characterized in that the lactic acid bacteria in step (2) is one or more lactic acid bacteria selected from among Lactobacillus, Bifidobacterium and Streptococcus. According to claim 4,
The lactic acid bacteria are Lactobacillus plantarum , Lactobacillus rhamnosus , Bifidobacterium animalis ssp. lactis , Bifidobacterium longum , and Streptococcus A method for producing double-coated red ginseng granules, characterized in that at least one lactic acid bacteria selected from Streptococcus thermophilus . According to claim 1,
The lactic acid bacteria killed in step (2) are Lactobacillus plantarum , Lactobacillus rhamnosus , Bifidobacterium animalis ssp. lactis , Bifidobacterium longum ( Bifidobacterium longum ) and Streptococcus thermophilus ( Streptococcus thermophilus ) A method for producing double-coated red ginseng granules, characterized in that dead cells of one or more types of lactic acid bacteria selected from, and dead cells of Enterococcus faecalis. According to claim 1,
In the first coating solution of step (2), red ginseng concentrate, enzyme, dead lactic acid bacteria, and live lactic acid bacteria are mixed in a weight ratio of 100: 5-10: 1-3: 1-3 Preparation of double-coated red ginseng granules method. According to claim 1,
The fermentation in step (2-1) is a method for producing double-coated red ginseng granules, characterized in that the first coating solution is stirred at 20 to 30 ° C. for 1 to 20 hours at 10 to 50 rpm. According to claim 1,
The fluidized bed coating granulator in steps (3) and (5)
The intake air temperature is 60 to 90 ℃, the fluidizing powder temperature is 40 to 50 ℃, the spray air pressure for fluidization is 2.8 to 3.2 kg / ㎠, and the input rate of the first coating solution or the second coating solution is 1 to 200 Method for producing double-coated red ginseng granules, characterized in that g / min. According to claim 1,
The natural polysaccharide in step (4) is at least two gums selected from guar gum, xanthan gum, gum arabic, cellulose gum, tragacanth gum, rogers bean gum, karaya gum, ghatti gum, tara gum and konjac gum Manufacturing method of double-coated red ginseng granules. According to claim 10,
The natural polysaccharide is a method for producing double-coated red ginseng granules, characterized in that gellan gum and gum arabic are mixed in a weight ratio of 1: 1-3. According to claim 1,
The double-coated red ginseng granules have a total content of ginsenosides Rb1, Rg1 and Rg3 of at least 7.0 mg/g, a content of ginsenoside F2 of at least 5.0 mg/g, and a content of ginsenoside compound K of at least 4.0 mg/g. Method for producing double-coated red ginseng granules, characterized in that mg / g or more. It is prepared according to the method of any one of claims 1, 2, 4 to 12, and the total of ginsenosides Rb1, Rg1 and Rg3, ginsenoside F2 and ginsenoside compound K content is increased, , Double-coated red ginseng granules with improved preservation.