CN115349409A - Method for improving quality of cabbage seedlings - Google Patents
Method for improving quality of cabbage seedlings Download PDFInfo
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- CN115349409A CN115349409A CN202211078397.7A CN202211078397A CN115349409A CN 115349409 A CN115349409 A CN 115349409A CN 202211078397 A CN202211078397 A CN 202211078397A CN 115349409 A CN115349409 A CN 115349409A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/15—Leaf crops, e.g. lettuce or spinach
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Soil Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention provides a method for improving the quality of cabbage seedlings, and belongs to the technical field of vegetable cultivation. By adopting the treatment method, the growth amount of the cabbage seedling can be remarkably improved when the sugar alcohol calcium solution is diluted by 1000 times and red and blue light is red light =1:2 (R: B = 1:2) in a red and blue light ratio, the chlorophyll content of the cabbage seedling can be remarkably improved when the sugar alcohol calcium solution is diluted by 2000 times and the red light is red light =1:2 (R: B = 1:2) in a red and blue light ratio, and the antioxidant capacity of the cabbage seedling can be remarkably improved when the sugar alcohol calcium solution is diluted by 2000 times and the red and blue light is red light =2:1 (R: B = 2:1) in a red and blue light ratio. The method can solve the problems of complex method for improving the quality of the cabbage seedling in the prior art and high cost, can directionally improve the quality of the cabbage seedling according to different requirements, and is simple and convenient to operate and low in cost.
Description
Technical Field
The invention relates to the technical field of vegetable cultivation, in particular to a method for improving the quality of cabbage seedlings.
Background
The flowering cabbage is also called a brassica, brassica of cruciferae, and a primary and secondary herbaceous plant. Is suitable for being sowed in warm areas in the south and is one of special vegetables in the south of China. Can be sowed all the year round, and is cultivated all over the world.
Miao vegetable is a novel vegetable which develops the fastest in recent years. The young seedlings are taken as edible objects, generally, the young seedlings are harvested when the plants grow to 3-5 leaves and the plant height is 10-15 cm. As the seedling vegetable growth process is short, and no medicament is required to be sprayed to prevent and control plant diseases and insect pests, the seedling vegetable is favored by consumers with green safety, comprehensive nutrition and the like. The seedling vegetable can be produced all the year round, and can be cultivated without soil or with soil. The substrate has the characteristics of good air permeability, strong buffering capacity, low investment, low cost and the like, and is generally adopted in annual seedling vegetable production.
The research on the quality of the cabbage seedlings generally focuses on the aspects of culture substrates, fertilizer application and the like, but the processing means is complex, the cost is high, and the method is not suitable for industrial application.
Disclosure of Invention
The invention aims to provide a method for improving the quality of a cabbage seedling, and aims to solve the problems of complexity and high cost of the method for improving the quality of the cabbage seedling in the prior art.
In order to achieve the above object, the present invention provides the following technical solutions:
a method for improving the quality of the cabbage seedling comprises the steps of soaking the cabbage seedling seeds in sugar alcohol calcium solution, culturing the soaked seeds in shade for 2-6 days, and then culturing the seeds under the condition of red and blue light irradiation;
the initial concentration of the sugar alcohol calcium solution is 200-250 g/L, and the dilution multiple is 800-2200 times;
the illumination intensity ratio of red light to blue light in the red light and the blue light is 1.5-2.5.
Preferably, the sugar alcohol calcium solution is a sorbitol chelated calcium solution.
Preferably, the seed soaking time is 3-5 h.
Preferably, the duration of the red and blue light irradiation is 8-12 h/d.
Preferably, the total illumination intensity of the red light and the blue light is 50-80 mu mol.m -2 ·s -1 。
Preferably, the wavelength of the red light is 650 to 670nm, and the wavelength of the blue light is 450 to 470nm.
Preferably, when the growth amount of the cabbage seedling is increased, the dilution multiple of the sugar alcohol calcium solution is 800-1200, and the illumination intensity ratio of red light to blue light in the red and blue light is 1.5-2.5.
Preferably, when the chlorophyll content of the cabbage seedling is increased, the dilution multiple of the sugar alcohol calcium solution is 1800-2200 times, and the illumination intensity ratio of red light to blue light in the red and blue light is 1.5-2.5.
Preferably, when the antioxidant capacity of the cabbage seedling is improved, the dilution multiple of the sugar alcohol calcium solution is 1800-2200 times, and the illumination intensity ratio of red light to blue light in the red and blue light is 1.5-2.5.
The invention has the technical effects and advantages that:
the method for improving the quality of the cabbage seedling can directionally improve the quality of the cabbage seedling according to different requirements, and is simple and convenient to operate and low in cost. By adopting the treatment method, the growth amount of the cabbage seedling can be remarkably improved when the sugar alcohol calcium solution is diluted by 1000 times and red and blue light is red light =1:2 (R: B = 1:2) in a red and blue light ratio, the chlorophyll content of the cabbage seedling can be remarkably improved when the sugar alcohol calcium solution is diluted by 2000 times and the red light is red light =1:2 (R: B = 1:2) in a red and blue light ratio, and the antioxidant capacity of the cabbage seedling can be remarkably improved when the sugar alcohol calcium solution is diluted by 2000 times and the red and blue light is red light =2:1 (R: B = 2:1) in a red and blue light ratio.
Detailed Description
The invention provides a method for improving the quality of cabbage seedling, which comprises the steps of soaking cabbage seedling seeds in sugar alcohol calcium solution, culturing in shade for 2-6 days after seed soaking, and then culturing under the condition of red and blue light irradiation; the sugar alcohol calcium solution is preferably sorbitol calcium chelate solution, the initial concentration of the sorbitol calcium chelate solution is preferably 200-250 g/L, and more preferably 220-230 g/L, the dilution multiple of the sorbitol calcium chelate solution is 800-2200 times, and preferably 1000-2000 times, and the pH of the sorbitol calcium chelate solution is preferably 6-7, and more preferably 6.4-6.8; in the seed soaking process: the mass-volume ratio of the cabbage seedling seeds to the sugar alcohol calcium solution is preferably 1:8-12, the seed soaking time is preferably 3-5 h, and the seed soaking temperature is preferablyIs 20 to 25 ℃; the light-shading cultivation is preferably carried out in a cultivation disc after seed soaking for 2-6 days, preferably 3-5 days, and 1-2 layers of gauze are preferably paved in the cultivation disc; culturing under the condition of red and blue light irradiation after shading culture, wherein the duration of the red and blue light irradiation is preferably 8-12 h/d, and more preferably 9-11 h/d; the total illumination intensity of the red light and the blue light is preferably 50-80 mu mol.m -2 ·s -1 More preferably 50 to 80. Mu. Mol/m -2 ·s -1 (ii) a The wavelength of the red light is preferably 650-670 nm, the wavelength of the blue light is preferably 450-470 nm, and the illumination intensity ratio of the red light to the blue light in the red light and the blue light is 1.5-2.5, preferably 1.8-2.2; the invention has no special requirements on other conditions in the cultivation process and can meet the requirement of the growth of the cabbage seedling.
In the invention, when the growth amount of the cabbage seedling is increased, the dilution multiple of the sugar alcohol calcium solution is preferably 800-1200, more preferably 900-1100, and the illumination intensity ratio of red light to blue light in the red light and the blue light is preferably 1.5-2.5, more preferably 1.8-2.2; when the chlorophyll content of the cabbage seedling vegetable is increased, the dilution multiple of the sugar alcohol calcium solution is preferably 1800-2200 times, more preferably 1900-2100 times, and the illumination intensity ratio of red light to blue light in the red light and the blue light is preferably 1.5-2.5, more preferably 1.8-2.2; when the antioxidant capacity of the cabbage seedling is improved, the dilution multiple of the sugar alcohol calcium solution is preferably 1800-2200 times, more preferably 1900-2100, and the illumination intensity ratio of red light to blue light in the red light and the blue light is preferably 1.5-2.5, more preferably 1.8-2.2.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Calcium alditol (sorbitol chelated calcium, ((C) 6 H 14 O 6 ) 2 Ca 3 ) CaO:225g/L, pH 6.7) solution, soaking seeds for 4h at room temperature 23 ℃ (the weight ratio of the solution to the seeds is 10Spreading in plastic culture dish with 1 layer of gauze, culturing in shade for 4 days, and culturing under irradiation of red light and blue light (red light LED 660 + -10 nm, blue light LED 460 + -10 nm) with total light intensity of 60 μmol · m -2 ·s -1 Red light to blue light =1:2 (R: B = 1:2) was used for the ratio. Illumination 8 per day: 00 to 18:00, 10h in total.
Example 2
Calcium alditol (sorbitol chelated calcium, ((C) 6 H 14 O 6 ) 2 Ca 3 ) CaO:225g/L, pH 6.7), soaking seeds for 3h at room temperature 23 ℃ (the weight ratio of the solution to the seeds is 10 -2 ·s -1 Red light =1:2 (R: B = 1:2) was used in the ratio. Illumination 8 per day: 00 to 18:00, 10h in total.
Example 3
Calcium alditol (sorbitol chelated calcium, ((C) 6 H 14 O 6 ) 2 Ca 3 ) CaO:225g/L, pH 6.7), soaking seeds for 5h at room temperature 23 ℃ (the weight ratio of the solution to the seeds is 10 -2 ·s -1 Red light to blue light =2:1 (R: B = 2:1) was used for the ratio. Illumination 8 per day: 00 to 18:00, for 10h.
Comparative example 1
The other treatment method is the same as that of the example 1, the sugar alcohol calcium is replaced by clean water with the same amount, and the red and blue light proportion is replaced by 1:1.
Comparative example 2
The other processing method was the same as that of example 1 except that 2:1 was used instead of the red and blue light ratio.
Experimental example 1 measurement of growth amount
The cultivation of the cabbage seedling is carried out according to the methods of example 1, comparative example 1 and comparative example 2, the example 1 is used as an experimental group A, the comparative examples 1 and 2 are used as a control group (1) and a control group (2), each group is repeated three times, after cotyledons are completely unfolded (14 d), 10 plants are randomly selected from each treated net frame to detect the growth amount index, and the method is as follows:
plant height: measuring the distance from the seedling vegetable leaf to the root by using a standard ruler after sampling;
fresh weight and dry weight: after sampling, removing roots and seed coats, weighing the fresh weight of 10 seedling vegetables by using an electronic balance, then immediately subpackaging according to groups, drying for 7 hours in a drying oven at 70 ℃, cooling, and weighing each group of dry weight by using the electronic balance.
The results are shown in table 1 below:
TABLE 1 index of growth of cabbage seedling
According to the detection result, the treatment method in the embodiment 1 can obviously improve the growth amount of the cabbage seedling, and the effect is better than that of the control group.
Experimental example 2 photosynthetic pigment content measurement
The cultivation of the cabbage seedling is carried out according to the methods of example 2, example 1 and example 3, the example 2 is used as an experimental group B, the examples 1 and example 3 are used as a control group (3) and a control group (4), each group is repeated three times, and after cotyledons are completely unfolded (14 d), plants are randomly selected to detect the photosynthetic pigment content by the following method: weighing 0.2 Ag blade, soaking in 75% ethanol, and dark treating until the blade becomes white. Then the content of photosynthetic pigments was determined by acetone-ethanol mixing method (Zhang Xianzheng. Content determination of phytochlorophyll-acetone-ethanol mixed solution method [ J ]. Liaoning agricultural science, 1986 (03): 26-28.).
The results are shown in table 2 below:
TABLE 2 photosynthetic pigment content test results for cabbage seedling
According to the detection result, the processing method in the embodiment 2 can obviously improve the content of photosynthetic pigments of the cabbage seedling vegetable, and the effect is superior to that of the control group.
Experimental example 3 measurement of antioxidant ability
The cultivation of cabbage seedling is carried out according to the methods of example 3 and comparative example 2, the method of example 3 is used as an experimental group C, the method of comparative example 2 is used as a control group (5), each group is repeated three times, and after cotyledons are completely unfolded (14 d), plants are randomly selected to detect the polyphenol content, the flavonoid content and the FRAP antioxidant capacity, and the method comprises the following steps: polyphenol content was determined by the Folin phenol method (Tadolin B, juliano C, piu L, et al. Reverse inhibition of lipid oxidation [ J ]. Free radial Research,2000, 33 (1): 105-114.), by the Mashiba method for determining flavonoid content (Ling Guanting. Antioxidant food and health [ M ]. Beijing: chemical industry Press, 2004), by the Benzie method for determining FRAP antioxidant capacity (Benzie I F, strain J. Ferrite reduction/antioxidant power assay: direct measure assay of total antioxidant activity of biological fluids and modified polypeptides for biological activity assay of biological activity and process of biological activity, J. Biological assay of biological activity and biological activity, 27. J. 15. Method of et al. (1999).
The results are shown in table 3 below:
TABLE 3 measurement results of antioxidant ability of cabbage seedling
According to the detection result, the processing method in the embodiment 3 can obviously improve the oxidation resistance of the cabbage seedling, and the effect is superior to that of a control group.
From the above examples, it can be seen that, by using the treatment method of the present invention, the growth amount of the cabbage seedling can be significantly increased when the sugar alcohol calcium solution is diluted 1000 times, and the red-blue light ratio is red light =1:2 (R: B = 1:2), the chlorophyll content of the cabbage seedling can be significantly increased when the sugar alcohol calcium solution is diluted 2000 times, and the red-blue light ratio is red light: blue light =1:2 (R: B = 1:2), and the antioxidant capacity of the cabbage seedling can be significantly increased when the sugar alcohol calcium solution is diluted 2000 times, and the red-blue light ratio is red light: blue light =2:1 (R: B = 2:1). The invention can directionally improve the quality of the cabbage seedling aiming at different requirements, and has simple and convenient operation and lower cost.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (9)
1. A method for improving the quality of cabbage seedling is characterized in that sugar alcohol calcium solution is adopted to soak seeds of cabbage seedling, the seeds are cultivated for 2 to 6 days in a shading mode after being soaked, and then cultivation is carried out under the condition of red and blue light irradiation;
the initial concentration of the sugar alcohol calcium solution is 200-250 g/L, and the dilution multiple is 800-2200 times;
the illumination intensity ratio of red light to blue light in the red and blue light is 1.5-2.5.
2. The method for improving the quality of cabbage seedlings as claimed in claim 1, wherein the sugar alcohol calcium solution is a sorbitol chelated calcium solution.
3. The method for improving the quality of the cabbage seedlings as claimed in claim 2, wherein the seed soaking time is 3-5 hours.
4. The method for improving quality of cabbage seedlings according to claim 3, wherein the red and blue light irradiation is performed for 8 to 12 hours/d.
5. The method for improving the quality of cabbage seedlings as claimed in claim 4, wherein the total illumination intensity of the red light and the blue light is 50-80 μmol-m -2 ·s -1 。
6. The method for improving the quality of cabbage seedlings as claimed in claim 5, wherein the wavelength of the red light is 650-670 nm, and the wavelength of the blue light is 450-470 nm.
7. The method as claimed in any one of claims 1 to 6, wherein when the growth of the cabbage seedling is promoted, the dilution ratio of the sugar alcohol calcium solution is 800-1200, and the illumination intensity ratio of red light to blue light in the red and blue light is 1.5-2.5.
8. The method according to any one of claims 1 to 6, wherein when the chlorophyll content of the cabbage seedling is increased, the dilution factor of the sugar alcohol calcium solution is 1800-2200 times, and the illumination intensity ratio of red light to blue light in the red-blue light is 1.5-2.5.
9. The method as claimed in any one of claims 1 to 6, wherein when the antioxidant capacity of the cabbage seedling is improved, the dilution multiple of the sugar alcohol calcium solution is 1800-2200 times, and the illumination intensity ratio of red light to blue light in the red and blue light is 1.5-2.5.
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| CN107371735A (en) * | 2017-08-23 | 2017-11-24 | 华南农业大学 | It is a kind of to couple the method for carrying out vegetables production with nutrient solution using LED illumination, time |
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