CN115349409B - Method for improving quality of cabbage seedling - Google Patents
Method for improving quality of cabbage seedling Download PDFInfo
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- CN115349409B CN115349409B CN202211078397.7A CN202211078397A CN115349409B CN 115349409 B CN115349409 B CN 115349409B CN 202211078397 A CN202211078397 A CN 202211078397A CN 115349409 B CN115349409 B CN 115349409B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/15—Leaf crops, e.g. lettuce or spinach
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
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- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Botany (AREA)
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- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention provides a method for improving the quality of cabbage seedlings, and belongs to the technical field of vegetable cultivation. By adopting the treatment method, when the sugar alcohol calcium solution is diluted 1000 times and the red-blue light ratio adopts red light to blue light=1:2 (R: B=1:2), the growth amount of the cabbage seedling vegetable can be obviously improved, when the sugar alcohol calcium solution is diluted 2000 times and the red-blue light ratio adopts red light to blue light=1:2 (R: B=1:2), the chlorophyll content of the cabbage seedling vegetable can be obviously improved, and when the sugar alcohol calcium solution is diluted 2000 times and the red-blue light ratio adopts red light to blue light=2:1 (R: B=2:1), the oxidation resistance of the cabbage seedling vegetable can be obviously improved. The invention can overcome the problems of complex and high cost of the improvement method of the cabbage seedling quality in the prior art, can also directionally improve the cabbage seedling quality according to different requirements, and has simple and convenient operation and lower cost.
Description
Technical Field
The invention relates to the technical field of vegetable cultivation, in particular to a method for improving the quality of cabbage seedlings.
Background
The flowering cabbage is also called as a flowering cabbage, a brassica of cruciferae, a first and second year-old herb. Is suitable for being sowed in warm areas in the south and is one of special vegetables in the south of China. Can be sowed all the year round, and the introduction and cultivation are carried out in all the places of the world now.
The seedling vegetable is a novel vegetable with the fastest development speed in recent years. The young seedlings are taken as edible objects, and generally refer to young seedlings which are harvested after plants grow to 3-5 leaves and the plant height is 10-15 cm. Because the seedling vegetable has short growth process and does not need to spray any medicament to prevent and treat diseases and insect pests, the seedling vegetable is favored by consumers in green, safe, comprehensive in nutrition and the like. The seedling vegetable can be produced all the year round, and can be cultivated in soil or soil. The substrate has the characteristics of good air permeability, strong buffering capacity, low investment, low cost and the like, and is commonly used in annual seedling vegetable production.
The related researches on the quality of the cabbage seedlings generally concentrate on the aspects of cultivation substrates, fertilizer application and the like, but the treatment means are complex, the cost is high, and the method is not suitable for industrial application.
Disclosure of Invention
The invention aims to provide a method for improving the quality of cabbage seedlings, which aims to solve the problems of complex and high cost of the method for improving the quality of cabbage seedlings in the prior art.
In order to achieve the above object, the present invention provides the following technical solutions:
a method for improving the quality of cabbage seedling vegetable adopts sugar alcohol calcium solution to soak the seeds of cabbage seedling vegetable, and after soaking, the seeds are cultivated for 2-6 days in a shading mode, and then cultivated under the condition of red and blue light irradiation;
the initial concentration of the sugar alcohol calcium solution is 200-250 g/L, and the dilution factor is 800-2200 times;
the illumination intensity ratio of the red light to the blue light in the red light to the blue light is 1.5-2.5:1.5-2.5.
Preferably, the sugar alcohol calcium solution is sorbitol chelated calcium solution.
Preferably, the seed soaking time is 3-5 hours.
Preferably, the duration of the red and blue light irradiation is 8-12 h/d.
Preferably, the total illumination intensity of the red and blue light is 50-80 mu mol.m -2 ·s -1 。
Preferably, the wavelength of the red light is 650-670 nm, and the wavelength of the blue light is 450-470 nm.
Preferably, when the growth amount of the cabbage seedling is increased, the dilution factor of the sugar alcohol calcium solution is 800-1200, and the illumination intensity ratio of red light to blue light in the red and blue light is 1:1.5-2.5.
Preferably, when the chlorophyll content of the cabbage is improved, the dilution factor of the sugar alcohol calcium solution is 1800-2200, and the illumination intensity ratio of red light to blue light in the red and blue light is 1:1.5-2.5.
Preferably, when the oxidation resistance of the cabbage is improved, the dilution factor of the sugar alcohol calcium solution is 1800-2200, and the illumination intensity ratio of red light to blue light in the red and blue light is 1.5-2.5:1.
The invention has the technical effects and advantages that:
the method for improving the quality of the cabbage seedlings can directionally improve the quality of the cabbage seedlings according to different requirements, and is simple and convenient to operate and low in cost. By adopting the treatment method, when the sugar alcohol calcium solution is diluted 1000 times and the red-blue light ratio adopts red light to blue light=1:2 (R: B=1:2), the growth amount of the cabbage seedling vegetable can be obviously improved, when the sugar alcohol calcium solution is diluted 2000 times and the red-blue light ratio adopts red light to blue light=1:2 (R: B=1:2), the chlorophyll content of the cabbage seedling vegetable can be obviously improved, and when the sugar alcohol calcium solution is diluted 2000 times and the red-blue light ratio adopts red light to blue light=2:1 (R: B=2:1), the oxidation resistance of the cabbage seedling vegetable can be obviously improved.
Detailed Description
The invention provides a method for improving the quality of cabbage seedling, which comprises the steps of soaking seeds of cabbage seedling with a sugar calcium alkoxide solution, shading and culturing for 2-6 days after soaking seeds, and then culturing under the condition of red and blue light irradiation; the sugar alcohol calcium solution is preferably a sorbitol chelate calcium solution, the initial concentration of the sorbitol chelate calcium solution is preferably 200-250 g/L, more preferably 220-230 g/L, the dilution factor of the sorbitol chelate calcium solution is 800-2200 times, preferably 1000-2000 times, and the pH of the sorbitol chelate calcium solution is preferably 6-7, more preferably 6.4-6.8; during the seed soaking process: the mass volume ratio of the cabbage seeds to the sugar alcohol calcium solution is preferably 1:8-12, the seed soaking time is preferably 3-5 h, and the seed soaking temperature is preferably 20-25 ℃; the invention is cultivated for 2 to 6 days, preferably 3 to 5 days in a shading way after seed soaking, the shading cultivation is preferably carried out in a cultivation disc, and 1 to 2 layers of gauze are preferably paved in the cultivation disc; culturing under red and blue light irradiation conditions after shading culturing, wherein the time length of the red and blue light irradiation is preferably 8-12 h/d, and more preferably 9-11 h/d; the total illumination intensity of the red and blue light is preferably 50-80 mu mol.m -2 ·s -1 More preferably 50 to 80. Mu. Mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the The wavelength of the red light is preferably 650-670 nm, the wavelength of the blue light is preferably 450-470 nm, and the illumination intensity ratio of the red light to the blue light in the red-blue light is 1.5-2.5:1.5-2.5, preferably 1.8-2.2:1.8-2.2; the invention has no special requirement on other conditions in the cultivation process, and can meet the requirement of the growth of the cabbage seedling.
In the invention, when the growth amount of the cabbage seedling is improved, the dilution factor of the sugar alcohol calcium solution is preferably 800-1200, more preferably 900-1100, and the illumination intensity ratio of red light to blue light in the red light and the blue light is preferably 1:1.5-2.5, more preferably 1:1.8-2.2; when the chlorophyll content of the cabbage is improved, the dilution factor of the sugar alcohol calcium solution is preferably 1800-2200 times, more preferably 1900-2100 times, and the illumination intensity ratio of red light to blue light in the red and blue light is preferably 1:1.5-2.5, more preferably 1:1.8-2.2; when the oxidation resistance of the cabbage is improved, the dilution factor of the sugar alcohol calcium solution is preferably 1800-2200 times, more preferably 1900-2100, and the illumination intensity ratio of red light to blue light in the red and blue light is preferably 1.5-2.5:1, more preferably 1.8-2.2:1.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Calcium sugar alkoxide (sorbitol chelate calcium, ((C) 6 H 14 O 6 ) 2 Ca 3 ) CaO:225g/L, pH 6.7) solution is diluted 1000 times, seed soaking is carried out for 4 hours at room temperature of 23 ℃ (the weight ratio of the solution to the seeds is 10:1), the seeds after seed soaking are evenly spread in a plastic culture dish paved with 1 layer of gauze, shading culture is carried out for 4 days, then, cultivation is carried out under the irradiation of red and blue light (red light LED 660+/-10 nm and blue light LED 460+/-10 nm), and the total light intensity is 60 mu mol.m -2 ·s -1 The ratio was red light to blue light=1:2 (R: b=1:2). Illumination per day 8: 00-18: 00 for 10 hours.
Example 2
Calcium sugar alkoxide (sorbitol chelate calcium, ((C) 6 H 14 O 6 ) 2 Ca 3 ) CaO:225g/L, pH 6.7) solution is diluted 2000 times, seed soaking is carried out at room temperature of 23 ℃ for 3h (the weight ratio of the solution to the seeds is 10:1), the seeds after seed soaking are evenly spread in a plastic culture dish paved with 1 layer of gauze, shading culture is carried out for 4 days, then, cultivation is carried out under the irradiation of red and blue light (red light LED 660+/-10 nm and blue light LED 460+/-10 nm), and the total light intensity is 60 mu mol.m -2 ·s -1 The ratio was red light to blue light=1:2 (R: b=1:2). Illumination per day 8: 00-18: 00 for 10 hours.
Example 3
Calcium sugar alkoxide (sorbitol chelate calcium, ((C) 6 H 14 O 6 ) 2 Ca 3 ) CaO:225g/L, pH 6.7) solution is diluted 2000 times, seed soaking is carried out at room temperature of 23 ℃ for 5h (the weight ratio of the solution to the seeds is 10:1), the seeds after seed soaking are evenly spread in a plastic culture dish paved with 1 layer of gauze, shading culture is carried out for 4 days, then, cultivation is carried out under the irradiation of red and blue light (red light LED 660+/-10 nm and blue light LED 460+/-10 nm), and the total light intensity is 60 mu mol.m -2 ·s -1 The ratio was red light to blue light=2:1 (R: b=2:1). Illumination per day 8: 00-18: 00 for 10 hours.
Comparative example 1
Other treatment modes are the same as in example 1, the calcium sugar alkoxide is replaced by equivalent clean water, and the ratio of red light to blue light is replaced by 1:1.
Comparative example 2
Other processing methods are the same as in example 1, and the ratio of red light to blue light is replaced by 2:1.
Experimental example 1 growth detection
The cultivation of the cabbage is carried out according to the methods of example 1, comparative example 1 and comparative example 2, the example 1 is used as an experimental group A, the comparative example 1 and comparative example 2 are used as a control group (1) and a control group (2), each group is repeated three times, and after cotyledons are completely unfolded (14 d), 10 plants of the detection growth indexes are randomly taken from each treated net frame, and the method is as follows:
plant height: measuring the distance from Miao Caizi leaves to roots by using a standard ruler after sampling;
fresh weight and dry weight: removing roots and seed coats after sampling, weighing the fresh weight of 10 seedling vegetables by an electronic balance, immediately split-charging according to the groups, drying in a baking oven at 70 ℃ for 7 hours, and weighing each group of dry weight by the electronic balance after cooling.
The results are shown in table 1 below:
TABLE 1 results of measurement of the growth index of the cabbage
From the detection results, the treatment method in the embodiment 1 can significantly improve the growth amount of the cabbage and the effect is better than that of the control group.
Experimental example 2 photosynthetic pigment content detection
The cultivation of the cabbage is carried out according to the methods of example 2, example 1 and example 3, example 2 is used as an experimental group B, example 1 and example 3 are used as a control group (3) and a control group (4), each group is repeated three times, and after cotyledons are completely unfolded (14 d), plants are randomly taken to detect the photosynthetic pigment content, and the method is as follows: 0.2 gram of leaf is weighed, soaked in 75% ethanol and subjected to darkness until the leaf turns white. The photosynthetic pigment content was then determined using the acetone-ethanol mixture method (Zhang Xianzheng. Plant chlorophyll content determination-acetone-ethanol mixture method [ J ]. Liaoning agriculture science, 1986 (03): 26-28.).
The results are shown in Table 2 below:
TABLE 2 detection results of photosynthetic pigment content of cabbage
From the detection results, the treatment method in the embodiment 2 can obviously improve the photosynthetic pigment content of the cabbage seedling, and the effect is better than that of the control group.
Experimental example 3 antioxidant Capacity test
The cultivation of the cabbage is carried out according to the method of example 3, comparative example 2, example 3 being experimental group C, comparative example 2 being control group (5), each group being repeated three times, after the cotyledons are completely spread (14 d), the plants are randomly taken to detect the polyphenol content, flavonoid content and FRAP antioxidant capacity, by the following method: polyphenol content measurement the flavonoid content was measured by Mashiba method (Ling Guanting. Antioxidant foods and health [ M ]. Beijing: chemical industry Press, 2004) using Fu Lin Fenfa (Tadolini B, juliano C, piu L, et al, resveratrol inhibition of lipid peroxidation [ J ]. Free Radical Research,2000, 33 (1): 105-114), and FRAP antioxidant capacity was measured by the Benzie method (Benzie I F, strain J.Ferric reduction/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration [ J ]. Methods in Enzymology,1999, 299 (1): 15-27).
The results are shown in Table 3 below:
TABLE 3 results of antioxidant capacity test of cabbage
From the detection results, the treatment method in the embodiment 3 can significantly improve the oxidation resistance of the cabbage and the effect is better than that of the control group.
According to the embodiment, by adopting the treatment method, when the sugar alcohol calcium solution is diluted 1000 times and the red-blue light ratio is red light and blue light=1:2 (R: B=1:2), the growth amount of the cabbage seedling vegetables can be remarkably improved, when the sugar alcohol calcium solution is diluted 2000 times and the red-blue light ratio is red light and blue light=1:2 (R: B=1:2), the chlorophyll content of the cabbage seedling vegetables can be remarkably improved, and when the sugar alcohol calcium solution is diluted 2000 times and the red-blue light ratio is red light and blue light=2:1 (R: B=2:1), the oxidation resistance of the cabbage seedling vegetables can be remarkably improved. The invention can directionally improve the quality of the cabbage seedling according to different requirements, and has simple and convenient operation and lower cost.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (2)
1. A method for improving the quality of cabbage seedling is characterized in that a sugar calcium alkoxide solution is adopted to soak seeds of the cabbage seedling, the seeds are subjected to shading cultivation for 2-6 days after soaking, and then cultivation is carried out under the condition of red and blue light irradiation;
the initial concentration of the sugar alcohol calcium solution is 200-250 g/L;
the illumination intensity ratio of red light to blue light in the red light to the blue light is 1.5-2.5:1.5-2.5;
when the growth amount of the cabbage seedling is improved, the dilution factor of the sugar alcohol calcium solution is 800-1200;
when the chlorophyll content of the cabbage is improved, the dilution factor of the sugar alcohol calcium solution is 1800-2200 times;
when the oxidation resistance of the cabbage is improved, the dilution factor of the calcium sugar alcohol solution is 1800-2200 times;
the sugar alcohol calcium solution is sorbitol chelated calcium solution;
the seed soaking time is 3-5 hours;
the duration of the red and blue light irradiation is 8-12 h/d;
the total illumination intensity of the red and blue light is 50-80 mu mol.m -2 ·s -1 。
2. The method for improving the quality of cabbage seedlings according to claim 1, wherein the wavelength of red light is 650-670 nm, and the wavelength of blue light is 450-470 nm.
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| CN108901790A (en) * | 2018-06-13 | 2018-11-30 | 华南农业大学 | A method of full artificial light vegetables nursery is controlled using day light summation |
| CN110235710A (en) * | 2019-06-17 | 2019-09-17 | 福建省中科生物股份有限公司 | A kind of plant factor's cultural method of three-coloured amaranth |
| CN110169317A (en) * | 2019-06-17 | 2019-08-27 | 福建省中科生物股份有限公司 | A kind of plant factor's cultural method of red celery |
| CN113248310A (en) * | 2021-05-28 | 2021-08-13 | 广西产研院生物制造技术研究所有限公司 | Polyglutamic acid, sugar alcohol calcium aqua and preparation method thereof |
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