CN115340158B - Water quality improver for multi-line butterfly fish culture pond and preparation method thereof - Google Patents
Water quality improver for multi-line butterfly fish culture pond and preparation method thereof Download PDFInfo
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- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 29
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims abstract description 28
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- 241000382455 Angelica sinensis Species 0.000 claims abstract description 24
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- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims abstract description 22
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- 239000011734 sodium Substances 0.000 claims abstract description 21
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- 239000007788 liquid Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 5
- RJFAYQIBOAGBLC-BYPYZUCNSA-N Selenium-L-methionine Chemical compound C[Se]CC[C@H](N)C(O)=O RJFAYQIBOAGBLC-BYPYZUCNSA-N 0.000 claims description 3
- RJFAYQIBOAGBLC-UHFFFAOYSA-N Selenomethionine Natural products C[Se]CCC(N)C(O)=O RJFAYQIBOAGBLC-UHFFFAOYSA-N 0.000 claims description 3
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- 238000010298 pulverizing process Methods 0.000 claims description 3
- 229960002718 selenomethionine Drugs 0.000 claims description 3
- 229910052725 zinc Inorganic materials 0.000 claims description 3
- 239000011701 zinc Substances 0.000 claims description 3
- 241000698291 Rugosa Species 0.000 claims description 2
- 241000958507 Stropharia Species 0.000 claims description 2
- 238000000855 fermentation Methods 0.000 claims description 2
- 230000004151 fermentation Effects 0.000 claims description 2
- 238000010992 reflux Methods 0.000 claims description 2
- 238000003809 water extraction Methods 0.000 claims description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 8
- 229910052760 oxygen Inorganic materials 0.000 abstract description 8
- 239000001301 oxygen Substances 0.000 abstract description 8
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- 229940072205 lactobacillus plantarum Drugs 0.000 description 3
- 229910052709 silver Inorganic materials 0.000 description 3
- 239000004332 silver Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
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- 238000007796 conventional method Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- KMUONIBRACKNSN-UHFFFAOYSA-N potassium dichromate Chemical compound [K+].[K+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O KMUONIBRACKNSN-UHFFFAOYSA-N 0.000 description 2
- 241001061264 Astragalus Species 0.000 description 1
- 235000008734 Bergera koenigii Nutrition 0.000 description 1
- 239000004129 EU approved improving agent Substances 0.000 description 1
- 244000303040 Glycyrrhiza glabra Species 0.000 description 1
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 description 1
- 239000009636 Huang Qi Substances 0.000 description 1
- 240000001899 Murraya exotica Species 0.000 description 1
- 235000008766 Murraya exotica Nutrition 0.000 description 1
- 240000002393 Murraya koenigii Species 0.000 description 1
- 235000009696 Murraya paniculata Nutrition 0.000 description 1
- 241000276618 Perciformes Species 0.000 description 1
- 241001274202 Selenotoca Species 0.000 description 1
- 241001274979 Selenotoca multifasciata Species 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 235000006533 astragalus Nutrition 0.000 description 1
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- 238000002474 experimental method Methods 0.000 description 1
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Marine Sciences & Fisheries (AREA)
- Feed For Specific Animals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a water quality improver for a multi-line butterfly fish culture pond and a preparation method thereof, wherein the water quality improver comprises the following raw materials in parts by weight: 18-22 parts of sodium humate, 19-25 parts of angelica sinensis, 24-33 parts of pennisetum hydridum, 21-27 parts of geranium, 27-31 parts of lactobacillus rhamnosus, 15-20 parts of bacillus subtilis, 10-15 parts of schizochytrium limacinum powder, 3-5 parts of hexahydro beta-laurate, 12-15 parts of zinc selenomethionine, 17-22 parts of amino acid, 16-20 parts of sodium molybdate and 14-17 parts of hydroxy methionine iron. The water quality improver can effectively improve the water quality condition of a culture pond, inhibit the reproduction of harmful bacteria, improve the oxygen content in water, reduce the accumulation of harmful substances in culture and has stable water quality effect. But also can prevent or reduce the occurrence of the diseases of the multi-line butterfly fish, improve the immunity of the multi-line butterfly fish, promote the growth of the multi-line butterfly fish and improve the culture yield and quality of the multi-line butterfly fish.
Description
Technical Field
The invention relates to the field of multi-line butterfly fish culture, in particular to a water quality improver for a multi-line butterfly fish culture pond and a preparation method thereof.
Background
Silver drum fish, academic name multi-line butterfly fish (Selenotoca multifasciata), belonging to the order Perciformes the family of the the genus Chaptera (Selenotoca). The silver drum fish body is oblate and white in body color, and tens of black round spots are sparse on the abdomen at the two sides of the body surface, and the silver drum fish body is money-like and has unique morphology. However, the breeding process is easy to accumulate harmful substances, diseases are easy to occur, especially the incidence rate of streptococcicosis is high, the butterfly fish with the striae is easy to die, sometimes the incidence rate reaches more than 60%, and the yield and quality of the butterfly fish with the striae are seriously affected.
Disclosure of Invention
In view of the above, the present invention provides a water quality improver for a culture pond of multi-line butterfly fish and a preparation method thereof, which solves the above problems.
The technical scheme of the invention is realized as follows:
a water quality improver for a multi-line butterfly fish culture pond comprises the following raw materials in parts by weight: 18-22 parts of sodium humate, 19-25 parts of angelica sinensis, 24-33 parts of pennisetum hydridum, 21-27 parts of geranium, 27-31 parts of lactobacillus rhamnosus, 15-20 parts of bacillus subtilis, 10-15 parts of schizochytrium limacinum powder, 3-5 parts of hexahydro beta-laurate, 12-15 parts of zinc selenomethionine, 17-22 parts of amino acid, 16-20 parts of sodium molybdate and 14-17 parts of hydroxy methionine iron. Preferably, 20 parts of sodium humate, 22 parts of angelica sinensis, 28 parts of pennisetum hydridum, 25 parts of geranium, 29 parts of lactobacillus rhamnosus, 17 parts of bacillus subtilis, 13 parts of schizochytrium limacinum powder, 4 parts of hexahydro beta-laurate, 13 parts of zinc selenomethionine, 20 parts of amino acid, 18 parts of sodium molybdate and 15 parts of iron hydroxy methionine.
The preparation method of the water quality improver for the multi-line butterfly fish culture pond comprises the following steps:
(1) Pulverizing radix Angelicae sinensis, herba Phyllostachydis Henonis, and herba Erodii seu Geranii to obtain mixed Chinese medicinal powder, adding 5-10% v/v ethanol solution, and fermenting with lactobacillus rhamnosus and bacillus subtilis to obtain fermented Chinese medicinal mixture;
(2) Extracting the fermented traditional Chinese medicine in the step (1) with subcritical water, repeatedly extracting under reflux for 2-4 times, 10-20 minutes each time, and mixing the extracting solutions;
(3) Adding hexahydro beta-laurate, sodium humate, zinc selenomethionine, amino acid, sodium molybdate, iron hydroxy methionine, schizochytrium limacinum powder, the balance of lactobacillus rhamnosus and bacillus subtilis into the extracting solution in the step (2), and vibrating and mixing to obtain the water quality improver for the culture pond of the stropharia rugosa.
Further, in the step (1), the crushing is superfine crushing, and the particle size of the mixed traditional Chinese medicine powder is 100-200nm.
Further, in the step (1), the mass ratio of the Chinese angelica, the pennisetum hydridum, the geranium and the ethanol solution is 1:0.4-0.6.
Further, in the step (1), the fermentation time is 48-56h.
Further, in the step (2), the subcritical water extraction temperature is 138-140 ℃.
Further, in the step (2), the mass-volume ratio kg/L of the fermented traditional Chinese medicine to the water is 1:13-18.
Further, in the step (3), the shaking condition is shaking at 2000-2500rpm for 10-20min.
Compared with the prior art, the invention has the beneficial effects that:
aiming at the multi-line butterfly fish, the invention adopts angelica sinensis, pennisetum hydridum and geranium, combines selenomethionine zinc, sodium molybdate, hydroxy methionine iron, amino acid, hexahydro beta-laurate and the like, and combines a specific preparation process, so that the prepared water quality improver for the culture pond can not only effectively improve the water quality condition of the culture pond, inhibit the reproduction of harmful bacteria, improve the oxygen content in water, reduce the accumulation of harmful substances in the culture and has stable water quality effect. But also can prevent or reduce the occurrence of the diseases of the multi-line butterfly fish, improve the immunity of the multi-line butterfly fish, promote the growth of the multi-line butterfly fish and improve the culture yield and quality of the multi-line butterfly fish.
Detailed Description
In order to better understand the technical content of the present invention, the following provides specific examples to further illustrate the present invention.
The experimental methods used in the embodiment of the invention are conventional methods unless otherwise specified.
Materials, reagents, and the like used in the examples of the present invention are commercially available unless otherwise specified.
In the embodiment of the invention, the number of lactobacillus rhamnosus is Bio-67059, and the number of bacillus subtilis is Bio-58723.
Example 1
A water quality improver for a multi-line butterfly fish culture pond comprises the following raw materials in parts by weight: 18 parts of sodium humate, 19 parts of angelica, 33 parts of pennisetum hydridum, 21 parts of geranium, 27 parts of lactobacillus rhamnosus, 20 parts of bacillus subtilis, 10 parts of schizochytrium limacinum powder, 5 parts of hexahydro beta-laurate, 15 parts of zinc selenomethionine, 22 parts of amino acid, 20 parts of sodium molybdate and 14 parts of iron hydroxy methionine.
The preparation method of the water quality improver comprises the following steps:
(1) Taking Chinese angelica, pennisetum hydridum and geranium herb traditional Chinese medicinal materials, and superfine grinding the Chinese angelica, pennisetum hydridum and geranium herb traditional Chinese medicinal materials to 100-200nm to obtain mixed traditional Chinese medicinal powder, wherein the mass ratio of the mixed traditional Chinese medicinal powder to the feed liquid is 1:0.5 adding 8% v/v ethanol solution, and adding lactobacillus rhamnosus and bacillus subtilis in a third amount in the formula, and fermenting at 37+/-2 ℃ for 50 hours to obtain a fermented traditional Chinese medicine mixture;
(2) Placing the fermented traditional Chinese medicine mixture in the step (1) in subcritical water at 138-140 ℃ for extraction, wherein the mass volume ratio kg/L of the fermented traditional Chinese medicine and water is 1:15, repeatedly reflux-extracting for 3 times each for 15 minutes, and combining the extracting solutions;
(3) Adding hexahydro beta-laurate, sodium humate, zinc selenomethionine, amino acid, sodium molybdate, iron hydroxy methionine, schizochytrium limacinum powder, and the balance lactobacillus rhamnosus and bacillus subtilis into the extracting solution in the step (2), and vibrating for 15min under the condition of 2200rpm to obtain the water quality improver for the multi-line butterfly fish culture pond.
Example 2
A water quality improver for a multi-line butterfly fish culture pond comprises the following raw materials in parts by weight: 22 parts of sodium humate, 25 parts of angelica sinensis, 24 parts of pennisetum hydridum, 27 parts of geranium, 31 parts of lactobacillus rhamnosus, 15 parts of bacillus subtilis, 15 parts of schizochytrium limacinum powder, 3 parts of hexahydro beta-laurate, 12 parts of zinc selenomethionine, 17 parts of amino acid, 16 parts of sodium molybdate and 17 parts of iron hydroxy methionine.
The preparation method is identical to example 1, comprising the following steps:
(1) Taking Chinese angelica, pennisetum hydridum and geranium herb traditional Chinese medicinal materials, and superfine grinding the Chinese angelica, pennisetum hydridum and geranium herb traditional Chinese medicinal materials to 100-200nm to obtain mixed traditional Chinese medicinal powder, wherein the mass ratio of the mixed traditional Chinese medicinal powder to the feed liquid is 1:0.5 adding 8% v/v ethanol solution, and adding lactobacillus rhamnosus and bacillus subtilis in a third amount in the formula, and fermenting at 37+/-2 ℃ for 50 hours to obtain a fermented traditional Chinese medicine mixture;
(2) Placing the fermented traditional Chinese medicine mixture in the step (1) in subcritical water at 138-140 ℃ for extraction, wherein the mass volume ratio kg/L of the fermented traditional Chinese medicine and water is 1:15, repeatedly reflux-extracting for 3 times each for 15 minutes, and combining the extracting solutions;
(3) Adding hexahydro beta-laurate, sodium humate, zinc selenomethionine, amino acid, sodium molybdate, iron hydroxy methionine, schizochytrium limacinum powder, and the balance lactobacillus rhamnosus and bacillus subtilis into the extracting solution in the step (2), and vibrating for 15min under the condition of 2200rpm to obtain the water quality improver for the multi-line butterfly fish culture pond.
Example 3
A water quality improver for a multi-line butterfly fish culture pond comprises the following raw materials in parts by weight: 20 parts of sodium humate, 22 parts of angelica sinensis, 28 parts of pennisetum hydridum, 25 parts of geranium, 29 parts of lactobacillus rhamnosus, 17 parts of bacillus subtilis, 13 parts of schizochytrium limacinum powder, 4 parts of hexahydro beta-laurate, 13 parts of zinc selenomethionine, 20 parts of amino acid, 18 parts of sodium molybdate and 15 parts of iron hydroxy methionine.
The preparation method is identical to example 1, comprising the following steps:
(1) Taking Chinese angelica, pennisetum hydridum and geranium herb traditional Chinese medicinal materials, and superfine grinding the Chinese angelica, pennisetum hydridum and geranium herb traditional Chinese medicinal materials to 100-200nm to obtain mixed traditional Chinese medicinal powder, wherein the mass ratio of the mixed traditional Chinese medicinal powder to the feed liquid is 1:0.5 adding 8% v/v ethanol solution, and adding lactobacillus rhamnosus and bacillus subtilis in a third amount in the formula, and fermenting at 37+/-2 ℃ for 50 hours to obtain a fermented traditional Chinese medicine mixture;
(2) Placing the fermented traditional Chinese medicine mixture in the step (1) in subcritical water at 138-140 ℃ for extraction, wherein the mass volume ratio kg/L of the fermented traditional Chinese medicine and water is 1:15, repeatedly reflux-extracting for 3 times each for 15 minutes, and combining the extracting solutions;
(3) Adding hexahydro beta-laurate, sodium humate, zinc selenomethionine, amino acid, sodium molybdate, iron hydroxy methionine, schizochytrium limacinum powder, and the balance lactobacillus rhamnosus and bacillus subtilis into the extracting solution in the step (2), and vibrating for 15min under the condition of 2200rpm to obtain the water quality improver for the multi-line butterfly fish culture pond.
Example 4
A water quality improver for a multi-line butterfly fish culture pond, the formula of which is the same as that of example 3. The difference from example 3 is that the preparation method of the water quality improver comprises the following specific steps:
(1) Taking Chinese angelica, pennisetum hydridum and geranium herb traditional Chinese medicinal materials, and superfine grinding the Chinese angelica, pennisetum hydridum and geranium herb traditional Chinese medicinal materials to 100-200nm to obtain mixed traditional Chinese medicinal powder, wherein the mass ratio of the mixed traditional Chinese medicinal powder to the feed liquid is 1:0.4 adding 10% v/v ethanol solution, and adding lactobacillus rhamnosus and bacillus subtilis in a third amount in the formula, and fermenting at 37+/-2 ℃ for 48 hours to obtain a fermented traditional Chinese medicine mixture;
(2) Placing the fermented traditional Chinese medicine mixture in the step (1) in subcritical water at 138-140 ℃ for extraction, wherein the mass volume ratio kg/L of the fermented traditional Chinese medicine and water is 1:18, repeatedly reflux-extracting for 2 times each for 20 minutes, and combining the extracting solutions;
(3) Adding hexahydro beta-laurate, sodium humate, zinc selenomethionine, amino acid, sodium molybdate, iron hydroxy methionine, schizochytrium limacinum powder, and the balance lactobacillus rhamnosus and bacillus subtilis into the extracting solution in the step (2), and vibrating for 20min under the condition of 2000rpm to obtain the water quality improver for the multi-line butterfly fish culture pond.
Example 5
A water quality improver for a multi-line butterfly fish culture pond, the formula of which is the same as that of example 3. The difference from example 3 is that the preparation method of the water quality improver comprises the following specific steps:
(1) Taking Chinese angelica, pennisetum hydridum and geranium herb traditional Chinese medicinal materials, and superfine grinding the Chinese angelica, pennisetum hydridum and geranium herb traditional Chinese medicinal materials to 100-200nm to obtain mixed traditional Chinese medicinal powder, wherein the mass ratio of the mixed traditional Chinese medicinal powder to the feed liquid is 1:0.6 adding 5% v/v ethanol solution, and adding lactobacillus rhamnosus and bacillus subtilis in a third amount in the formula, and fermenting at 37+/-2 ℃ for 56 hours to obtain a fermented traditional Chinese medicine mixture;
(2) Placing the fermented traditional Chinese medicine mixture in the step (1) in subcritical water at 138-140 ℃ for extraction, wherein the mass volume ratio kg/L of the fermented traditional Chinese medicine and water is 1:13, repeatedly reflux-extracting for 4 times each for 10 minutes, and combining the extracting solutions;
(3) Adding hexahydro beta-laurate, sodium humate, zinc selenomethionine, amino acid, sodium molybdate, iron hydroxy methionine, schizochytrium limacinum powder, and the balance lactobacillus rhamnosus and bacillus subtilis into the extracting solution in the step (2), and vibrating for 10min under the condition of 2500rpm to obtain the water quality improver for the multi-line butterfly fish culture pond. Comparative example 1-A water quality improver for a culture pond of a Chaptera multiflora, differing from example 3 in that lactobacillus rhamnosus was replaced with an equivalent amount of lactobacillus plantarum (Bio-60181). The water quality improver comprises the following raw materials in parts by weight: 20 parts of sodium humate, 22 parts of angelica, 28 parts of pennisetum hydridum, 25 parts of geranium, 29 parts of lactobacillus plantarum, 17 parts of bacillus subtilis, 13 parts of schizochytrium limacinum powder, 4 parts of hexahydro beta-laurate, 13 parts of zinc selenomethionine, 20 parts of amino acid, 18 parts of sodium molybdate and 15 parts of iron hydroxy methionine.
Comparative example 2-A water quality improver for a culture pond of Murraya paniculata, which is different from example 3 in that radix Angelicae sinensis is replaced with equivalent amount of radix astragali, and pennisetum hydridum and herba Erodii seu Geranii are replaced with equivalent amount of Glycyrrhrizae radix. The weight portions of the components are 20 portions of sodium humate, 22 portions of astragalus, 53 portions of liquorice, 29 portions of lactobacillus plantarum, 17 portions of bacillus subtilis, 13 portions of schizochytrium limacinum powder, 4 portions of hexahydro beta-laurate, 13 portions of zinc selenomethionine, 20 portions of amino acid, 18 portions of sodium molybdate and 15 portions of hydroxy methionine iron.
Comparative example 3-A water quality improver for a culture pond of a multi-striated butterfly fish is different from example 3 in the ratio of raw materials. The water quality improver comprises the following raw materials in parts by weight: 20 parts of sodium humate, 15 parts of angelica sinensis, 20 parts of pennisetum hydridum, 15 parts of geranium, 29 parts of lactobacillus rhamnosus, 17 parts of bacillus subtilis, 13 parts of schizochytrium limacinum powder, 10 parts of hexahydro beta-laurate, 20 parts of zinc selenomethionine, 20 parts of amino acid, 25 parts of sodium molybdate and 20 parts of iron hydroxy methionine.
Comparative example 4-A water quality improver for a culture pond of a multi-striated butterfly fish is different from example 3 in the ratio of raw materials. The water quality improver comprises the following raw materials in parts by weight: 20 parts of sodium humate, 30 parts of angelica sinensis, 35 parts of pennisetum hydridum, 20 parts of geranium, 29 parts of lactobacillus rhamnosus, 17 parts of bacillus subtilis, 13 parts of schizochytrium limacinum powder, 5 parts of hexahydro beta-laurate, 10 parts of zinc selenomethionine, 20 parts of amino acid, 15 parts of sodium molybdate and 10 parts of iron hydroxy methionine.
Comparative example 5-A water quality improver for a culture pond of a multi-striated butterfly fish differs from example 3 in the ratio of raw materials. The water quality improver comprises the following raw materials in parts by weight: 20 parts of sodium humate, 20 parts of angelica sinensis, 20 parts of pennisetum hydridum, 20 parts of geranium, 20 parts of lactobacillus rhamnosus, 20 parts of bacillus subtilis, 20 parts of schizochytrium limacinum powder, 4 parts of hexahydro beta-laurate, 20 parts of zinc selenomethionine, 20 parts of amino acid, 20 parts of sodium molybdate and 20 parts of iron hydroxy methionine.
Comparative example 6-Water quality improver for a culture pond of Murray butterfly fish, which is different from example 3 in the preparation method, the specific steps are as follows: pulverizing Chinese angelica, pennisetum hydridum and geranium into 100 meshes to obtain mixed traditional Chinese medicine powder, and placing the mixed traditional Chinese medicine powder into water, wherein the mass volume ratio kg/L of the mixed traditional Chinese medicine powder to the water is 1:15, adding hexahydro beta-laurate, sodium humate, selenomethionine zinc, amino acid, sodium molybdate, hydroxy methionine iron, schizochytrium limacinum powder, lactobacillus rhamnosus and bacillus subtilis, and stirring and mixing to obtain the water quality improver.
Test example 1
Collecting the special culture pond wastewater of the multi-line butterfly fish, wherein the dissolved oxygen content of the water is 1.57mg/L, and the COD value is 241.5mg/L. Study groups 1 to 11 were set, and the water quality improvers of examples 1 to 5 and comparative examples 1 to 6 were used in correspondence with each other, with a dosage of 10g/L, 3 times of parallel tests per group, and a throughput of 10L per group. And detecting the water quality indexes after treatment, including dissolved oxygen, COD and the like. Wherein, the dissolved oxygen in water is measured by a portable dissolved oxygen meter. The COD of water quality adopts potassium dichromate as oxidant, and the chemical oxygen consumption is measured. The results are shown in Table 1 below:
| dissolved oxygen mg/L | CODmg/L | |
| Example 1 | 6.45 | 15.3 |
| Example 2 | 6.53 | 18.4 |
| Example 3 | 6.74 | 13.2 |
| Example 4 | 6.61 | 16.1 |
| Example 5 | 6.58 | 17.9 |
| Comparative example 1 | 6.06 | 34.5 |
| Comparative example 2 | 6.17 | 38.8 |
| Comparative example 3 | 6.26 | 36.8 |
| Comparative example 4 | 6.30 | 37.2 |
| Comparative example 5 | 5.27 | 31.3 |
| Comparative example 6 | 6.28 | 37.4 |
The results show that the water quality improving agents of examples 1 to 5 are better in water quality effect after treatment than those of comparative examples 1 to 6. The water quality improver effectively improves the dissolved oxygen content in water and reduces the COD value.
Test example 2
2021, 2 nd month, eastern city of Hainan provinceThe water quality treatment agent of examples 1-5 and comparative example 6 is respectively and correspondingly used in the water quality treatment agent of the examples 1-6, and each culture pond is 100m 2 The specification of the multi-line butterfly fish fries is 1-1.5cm, and the throwing density is 250 tails/m 3 The water level is about 1.0m, and on the basis of the conventional method, the water quality treating agent is periodically added in the cultivation process until the butterfly fish with the striae of the multiple is harvested, the water quality treating agent is added 1 time per month, and the adding amount of each time is 10g/L. After 3 months of cultivation, the morbidity and mortality of the Murraya koenigii are counted. The results are shown in Table 2 below:
the results show that the water quality modifier of the examples 1-5 is administered to effectively reduce the incidence rate of streptococcus mutans and obviously reduce the mortality rate of the streptococcus mutans.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Claims (8)
1. The water quality improver for the multi-line butterfly fish culture pond is characterized by comprising the following raw materials in parts by weight: 18-22 parts of sodium humate, 19-25 parts of angelica sinensis, 24-33 parts of pennisetum hydridum, 21-27 parts of geranium, 27-31 parts of lactobacillus rhamnosus, 15-20 parts of bacillus subtilis, 10-15 parts of schizochytrium limacinum powder, 3-5 parts of hexahydro beta-laurate, 12-15 parts of zinc selenomethionine, 17-22 parts of amino acid, 16-20 parts of sodium molybdate and 14-17 parts of hydroxy methionine iron; the preparation method of the water quality improver for the multi-line butterfly fish culture pond comprises the following steps:
(1) Pulverizing radix Angelicae sinensis, herba Phyllostachydis Henonis, and herba Erodii seu Geranii to obtain mixed Chinese medicinal powder, adding 5-10% v/v ethanol solution, and fermenting with lactobacillus rhamnosus and bacillus subtilis to obtain fermented Chinese medicinal mixture;
(2) Extracting the fermented traditional Chinese medicine mixture in the step (1) with subcritical water, repeatedly extracting under reflux for 2-4 times, 10-20 minutes each time, and mixing the extracting solutions;
(3) Adding hexahydro beta-laurate, sodium humate, selenomethionine zinc, amino acid, sodium molybdate, hydroxyl methionine iron, schizochytrium limacinum powder, the balance of lactobacillus rhamnosus and bacillus subtilis into the extracting solution in the step (2), and vibrating and mixing to obtain the water quality improver for the culture pond of the stropharia rugosa.
2. The water quality improver for the culture pond of the multi-line butterfly fish according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 20 parts of sodium humate, 22 parts of angelica sinensis, 28 parts of pennisetum hydridum, 25 parts of geranium, 29 parts of lactobacillus rhamnosus, 17 parts of bacillus subtilis, 13 parts of schizochytrium limacinum powder, 4 parts of hexahydro beta-laurate, 13 parts of zinc selenomethionine, 20 parts of amino acid, 18 parts of sodium molybdate and 15 parts of iron hydroxy methionine.
3. The water quality improver for a culture pond of multi-line butterfly fish according to claim 1, wherein in the step (1), the crushing is superfine crushing, and the size fraction of the mixed traditional Chinese medicine powder is 100-200nm.
4. The water quality improver for the culture pond of the multi-line butterfly fish according to claim 1, wherein in the step (1), the mass ratio of the feed liquid of the angelica sinensis, the pennisetum hydridum, the geranium and the ethanol solution is 1:0.4-0.6.
5. The water quality improver for a culture pond of multi-striated butterfly fish according to claim 1, wherein in the step (1), the fermentation time is 48-56h.
6. The water quality improver for a culture pond of multi-line butterfly fish according to claim 1, wherein in the step (2), the subcritical water extraction temperature is 138-140 ℃.
7. The water quality improver for a culture pond of multi-line butterfly fish according to claim 1, wherein in the step (2), the mass-volume ratio kg/L of the fermented traditional Chinese medicine mixture and subcritical water is 1:13-18.
8. The water quality improver for a culture pond of multi-line butterfly fish according to claim 1, wherein in the step (3), the shaking condition is shaking at 2000-2500rpm for 10-20min.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103980104A (en) * | 2014-02-19 | 2014-08-13 | 广州英赛特生物技术有限公司 | hexahydrocolupulone monoesters, inner complex salts or monoester salts, and application as animal feed additive |
| CN110304737A (en) * | 2019-07-09 | 2019-10-08 | 常德市万惠生物科技有限公司 | A kind of compound probiotic and preparation method thereof |
| CN110451656A (en) * | 2019-07-17 | 2019-11-15 | 常德市万惠生物科技有限公司 | A kind of improver of water quality and preparation method thereof |
| CN110627225A (en) * | 2019-09-30 | 2019-12-31 | 云南省渔业科学研究院（云南省渔业病害防治和质量检测中心） | Water quality improver for aquaculture |
| CN112056252A (en) * | 2020-09-21 | 2020-12-11 | 通威股份有限公司 | Efficient culture method for culturing three batches of penaeus vannamei boone in one year |
| WO2021127120A1 (en) * | 2019-12-18 | 2021-06-24 | BiOWiSH Technologies, Inc. | Compositions and methods for inhibition of pathogenic bacteria in aquaculture |
| CN114304441A (en) * | 2022-01-10 | 2022-04-12 | 长沙兴嘉生物工程股份有限公司 | Organic trace element compound bag for fish and preparation method and application thereof |
| CN114468161A (en) * | 2022-02-25 | 2022-05-13 | 北京九州大地生物技术集团股份有限公司 | Fattening pig feed for producing selenium-rich pork and preparation method and feeding method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2859797A4 (en) * | 2012-06-07 | 2015-12-30 | J&B Bio Inc | Additive composition for shrimp feed and a method for producing the same |
-
2022
- 2022-06-27 CN CN202210736605.1A patent/CN115340158B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103980104A (en) * | 2014-02-19 | 2014-08-13 | 广州英赛特生物技术有限公司 | hexahydrocolupulone monoesters, inner complex salts or monoester salts, and application as animal feed additive |
| CN110304737A (en) * | 2019-07-09 | 2019-10-08 | 常德市万惠生物科技有限公司 | A kind of compound probiotic and preparation method thereof |
| CN110451656A (en) * | 2019-07-17 | 2019-11-15 | 常德市万惠生物科技有限公司 | A kind of improver of water quality and preparation method thereof |
| CN110627225A (en) * | 2019-09-30 | 2019-12-31 | 云南省渔业科学研究院（云南省渔业病害防治和质量检测中心） | Water quality improver for aquaculture |
| WO2021127120A1 (en) * | 2019-12-18 | 2021-06-24 | BiOWiSH Technologies, Inc. | Compositions and methods for inhibition of pathogenic bacteria in aquaculture |
| CN112056252A (en) * | 2020-09-21 | 2020-12-11 | 通威股份有限公司 | Efficient culture method for culturing three batches of penaeus vannamei boone in one year |
| CN114304441A (en) * | 2022-01-10 | 2022-04-12 | 长沙兴嘉生物工程股份有限公司 | Organic trace element compound bag for fish and preparation method and application thereof |
| CN114468161A (en) * | 2022-02-25 | 2022-05-13 | 北京九州大地生物技术集团股份有限公司 | Fattening pig feed for producing selenium-rich pork and preparation method and feeding method thereof |
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