CN115316551A - Laying hen feed containing fermented brewer's grains and preparation method and application thereof - Google Patents

Laying hen feed containing fermented brewer's grains and preparation method and application thereof Download PDF

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Publication number
CN115316551A
CN115316551A CN202211040789.4A CN202211040789A CN115316551A CN 115316551 A CN115316551 A CN 115316551A CN 202211040789 A CN202211040789 A CN 202211040789A CN 115316551 A CN115316551 A CN 115316551A
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grains
corn
fermented brewer
feed containing
brewer
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张家才
刘兆金
王江水
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GUANGDONG HAID GROUP CO Ltd
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GUANGDONG HAID GROUP CO Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/70Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75Feeding-stuffs specially adapted for particular animals for birds for poultry
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A23K10/38Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/105Aliphatic or alicyclic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/158Fatty acids; Fats; Products containing oils or fats
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/189Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/26Compounds containing phosphorus
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

The invention relates to a layer feed containing fermented brewer's grains, a preparation method and an application thereof, wherein the raw materials comprise, by weight, 40-55% of corn, 6-10% of wheat, 15-20% of soybean meal, 4-7% of dried whole corn distillers grains, 2-4% of sunflower seed meal, 9-10% of stone powder, 0.1-1% of vegetable oil, 2-3% of fermented brewer's grains, 1-2% of slurry-sprayed corn husks, 1-2% of calcium hydrogen phosphate and 1-2% of additives. The laying hen fed by the laying hen feed has the advantages of improved immunity, improved intestinal digestion, remarkably increased weight of produced eggs and reduced feed-egg ratio.

Description

Laying hen feed containing fermented brewer's grains and preparation method and application thereof
Technical Field
The invention relates to the technical field of laying hen feeds, and particularly relates to a laying hen feed containing fermented brewer's grains, and a preparation method and application thereof.
Background
The poultry breeding in the breeding industry is an important component, the breeding speed is high, the return of laying hens is rich, the consumption of feed is relatively low, and therefore the poultry breeding is popular among farmers. At present, with the popularization of artificial feeding, a plurality of laying hen feeds appear, but the nutrient components in the existing laying hen feed are not reasonable, the growth of the laying hen is unfavorable, and in the season of temperature mutation, such as spring and autumn season change, the laying hen often has watery diarrhea, the digestion and absorption of the laying hen on the feed are influenced, the feed conversion rate is reduced, and meanwhile, the laying hen is reduced due to the lack of nutrition.
Brewer's spent grain is a major by-product of the beer industry, accounting for 85% of the beer industry waste and by-products. The annual brewer's grain yield of China is over 1000 million tons, and the brewer's grain yield is mainly used in the feed industry and is also used in the food industry. The brewer's grain is rich in crude protein, balanced with amino acid, rich in dietary fiber, various in phenolic compounds, high in activity and the like. As a protein feed raw material, the brewer's grains have the defects of low protein content and high crude fiber content compared with the common protein raw materials. In order to alleviate this disadvantage, brewery mash may be fermented to obtain fermented brewery mash. The fermented brewer's grains are applied to the laying hen feed to improve the situation.
Disclosure of Invention
The invention provides a laying hen feed containing fermented brewer grains and a preparation method and application thereof, wherein the laying hen feed can improve the reproductive performance of laying hens, improve the immunity of the laying hens and increase the digestive capacity of intestinal tracts, reduce the feed cost and improve the net breeding income.
The scheme for solving the technical problems is as follows: a laying hen feed containing fermented brewer's grains comprises, by weight, 40-55% of corn, 6-10% of wheat, 15-20% of soybean meal, 4-7% of dried whole corn grains (corn DDGS), 2-4% of sunflower seed meal, 9-10% of stone powder, 0.1-1% of vegetable oil, 2-3% of fermented brewer's grains, 1-2% of spray corn bran, 1-2% of calcium hydrogen phosphate and 1-2% of additives.
Preferably, the ingredients of the additive comprise 17.9% L-lysine sulfate, 10.7% DL-methionine, 21.4% sodium chloride, 4.3% choline chloride, 1.1% phytase, 1.4% betaine, 7.1% mineral element premix, 1.1% vitamin premix, 6.4% L-threonine, 5.7% sodium humate, 22.9% rice hull flour.
Preferably, the content of crude protein in the soybean meal is 43wt%.
Preferably, the purity of the L-lysine sulfate is 70%, and the purity of the choline chloride is 60%.
Preferably, the raw materials comprise, by weight, 45-50% of corn, 8-9% of wheat, 18-19% of soybean meal, 5-6% of corn DDGS, 2.8-3.3% of sunflower seed meal, 9-9.5% of stone powder, 0.2-0.5% of vegetable oil, 2.0-2.5% of fermented brewer's grains, 1.2-1.8% of gunite corn husks, 1-1.5% of calcium hydrogen phosphate and 1-2% of additives.
Preferably, the raw materials comprise 48.7 percent of corn, 8.4 percent of wheat, 18.5 percent of soybean meal, 6.0 percent of corn DDGS, 3.0 percent of sunflower seed meal, 9.2 percent of stone powder, 0.3 percent of vegetable oil, 2.0 percent of fermented brewer grains, 1.5 percent of spraying corn husks, 1 percent of calcium hydrophosphate and 1.4 percent of additives in percentage by weight; wherein the ingredients of the additive consist of 17.9% L-lysine sulphate, 10.7% DL-methionine, 21.4% sodium chloride, 4.3% choline chloride, 1.1% phytase, 1.4% betaine, 7.1% mineral element premix, 1.1% vitamin premix, 6.4% L-threonine, 5.7% sodium humate, 22.9% rice hull flour.
The preparation method of the laying hen feed containing the fermented brewer grains comprises the following steps: weighing the raw materials according to the weight ratio for later use; uniformly mixing calcium hydrogen phosphate and additives, and uniformly mixing with other raw materials to obtain the laying hen feed containing the fermented brewer's grains.
The layer feed containing the fermented brewer's grains is applied to layer breeding.
The invention has the beneficial effects that: the brewer's grain is rich in crude protein, balanced with amino acid, rich in dietary fiber, various in phenolic compounds and high in activity, can improve reproductive performance of female livestock and poultry when being applied to livestock and poultry feed, promotes balance of animal intestinal microbial population, reduces feed cost, does not influence laying rate of laying hens, obviously increases egg weight, reduces feed-egg ratio and increases intestinal digestive enzyme activity and immunocompetence of the laying hens after being applied to the laying hens fed with the feed prepared by introducing the fermented brewer's grain into a laying hen feed formula.
The foregoing is a summary of the present invention, and in order to provide a clear understanding of the technical means of the present invention and to be implemented in accordance with the present specification, the following is a preferred embodiment of the present invention and is described in detail below. Specific embodiments of the present invention are given by the following examples and their details.
Detailed Description
The principles and features of this invention are described below in conjunction with embodiments, which are included to explain the invention and not to limit the scope of the invention.
Example 1
A laying hen feed containing fermented brewer's grains comprises the following raw material components in a formula shown in a table 1:
TABLE 1 example 1 feed formula for laying hens
Figure BDA0003820243200000031
Figure BDA0003820243200000041
The layer feed prepared according to the formula is marked as a treatment group; and (3) preparing the laying hen feed by using the same amount of the sprayed corn bran instead of the fermented brewer grains according to the formula, and marking as a control group. And (5) carrying out a comparative test of the influence of the two groups of layer feed on the production performance of the layer.
(1) Design of animal experiment
1920 harland brown laying hens aged 34 weeks were selected and randomly divided into 2 groups of 4 groups of 240 chickens per group, with the initial laying rate of the test laying hens being 92.1%. The experimental pre-feeding period is 1 week, the positive feeding period is 4 weeks, the laying hens freely feed and drink water, and the light intensity is 16 hours and the darkness is 8 hours every day.
(2) Laying hen productivity
Taking the group as a unit, recording the feed intake, egg weight and egg laying number of the laying hens every day, and counting the residual feed amount every week. And calculating the average feed intake, average laying rate, average egg weight and feed-egg ratio of the laying hens every two weeks by taking the groups as units. The production performance data of the layers are shown in table 2. During the test period of 1-2 weeks, the egg weight of the laying hens in the treatment group is obviously increased, and the feed-egg ratio is obviously reduced.
TABLE 2 egg-laying hen production Performance
Figure BDA0003820243200000042
Figure BDA0003820243200000051
Note: the difference of the shoulder mark letters of the same row data represents significant difference (P < 0.05); the same row data without shoulder-marked letters or with the same shoulder-marked letters indicate no significant difference (P < 0.05), the same below.
(3) Detecting serum immune globulin and endotoxin of laying hen
After the experiment, 2 laying hens are randomly selected by each group, blood is collected from the wing veins, the blood is centrifuged at low temperature, and upper serum is taken and stored at low temperature for later use. And respectively detecting the content of IgA, igG, igM and ET in the serum by using an enzyme-linked immunosorbent assay. Data on serum immunoglobulin (IgA, igM, igG) and Endotoxin (ET) levels in layers are shown in table 3. The serum IgG content of the laying hens in the treated group is obviously higher than that of the control group, and the ET content is slightly lower than that of the control group.
TABLE 3 detection results of serum immunoglobulin and endotoxin of layer chicken
Figure BDA0003820243200000052
(4) Detection of chicken intestinal digestive enzyme activity
Randomly selecting 2 laying hens for each group, slaughtering, putting duodenal chyme into a freezing storage tube, quickly freezing in liquid nitrogen, and transferring to a-80 ℃ ultra-low temperature refrigerator for storage for later use. During detection, the chyme is taken out of a refrigerator at the temperature of-80 ℃, unfreezed on ice, and then centrifuged at the temperature of 4 ℃ to obtain supernatant, wherein the supernatant is used for detecting activities of chyme alpha-amylase, lipase and chymotrypsin. The activity data of the duodenal chyme digestive enzyme of the laying hens are shown in a table 4. The activities of alpha-amylase and lipase in duodenal chyme of the laying hens in the treatment group are obviously higher than those of the control group, and the chymotrypsin is slightly higher than that of the control group.
TABLE 4 detection results of intestinal digestive enzyme activity of egg-laying hens
Figure BDA0003820243200000061
As can be seen by comparing the control group test with the treatment group test, the immunity of the laying hens fed by the laying hen feed is improved, the intestinal digestion capability of the laying hens is improved, the weight of produced eggs is remarkably increased, and the feed-egg ratio is reduced.
Examples 2 to 4
The raw material component formulation of the layer chicken feed containing fermented brewer's grains prepared in examples 2-4 is shown in table 4:
table 4 examples 2-4 contents (wt%) of raw materials for layer chicken feed
Figure BDA0003820243200000062
Figure BDA0003820243200000071
The animal design test, the laying hen production performance, the test of serum immunoglobulin and endotoxin of laying hen, and the test of intestinal digestive enzyme activity of chicken related to the laying hen feed prepared in examples 2-4 are the same as those in example 1.
The laying performance of the laying hens in examples 2-4 is shown in table 5, and during the period of 1-2 weeks of the test, the egg weight of the laying hens in the treatment group is obviously increased, the feed-egg ratio is obviously reduced, but the feed-egg ratio is slightly higher than that of the laying hens in example 1.
TABLE 5 egg hen production Performance of examples 2-4
Figure BDA0003820243200000072
In examples 2-4, the serum immunoglobulin (IgA, igM, igG) and Endotoxin (ET) levels of the layers are shown in table 6, the IgG content in the layer serum of the treated group is significantly higher than that in the control group, the ET content is slightly lower than that in the control group, and the immunoglobulin level in the layer serum is slightly lower than that in example 1.
TABLE 6 EXAMPLES 2-4 detection results of serum immunoglobulin and endotoxin of egg-laying hens
Figure BDA0003820243200000081
The activity data of the chicken duodenal chyme digestive enzymes in examples 2 to 4 are shown in Table 7. In examples 2-4, the activities of alpha-amylase, lipase and chymotrypsin in duodenal chyme of the laying hens are slightly higher than those of a control group, and compared with the activity levels of the alpha-amylase, the lipase and the chymotrypsin in the laying hens in example 1, the activities of the alpha-amylase, the lipase and the chymotrypsin are slightly lower.
TABLE 7 EXAMPLE 2-4 detection results of intestinal digestive enzyme Activity in egg-laying hens
Figure BDA0003820243200000082
The foregoing is illustrative of the preferred embodiments of the present invention, and is not to be construed as limiting the invention in any way; as will be readily apparent to those skilled in the art from the disclosure herein, the present invention may be practiced without these specific details; however, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the scope of the invention as defined by the appended claims; meanwhile, any changes, modifications, and evolutions of the equivalent changes of the above embodiments according to the actual techniques of the present invention are still within the protection scope of the technical solution of the present invention.

Claims (8)

1. The laying hen feed containing fermented brewer's grains is characterized by comprising, by weight, 40-55% of corn, 6-10% of wheat, 15-20% of soybean meal, 4-7% of dried whole corn grains, 2-4% of sunflower seed meal, 9-10% of stone powder, 0.1-1% of vegetable oil, 2-3% of fermented brewer's grains, 1-2% of slurry-sprayed corn bran, 1-2% of calcium hydrogen phosphate and 1-2% of additives.
2. The fermented brewer's grain-containing layer feed according to claim 1, wherein the ingredients of said additive consist of 17.9% L-lysine sulfate, 10.7% DL-methionine, 21.4% sodium chloride, 4.3% choline chloride, 1.1% phytase, 1.4% betaine, 7.1% mineral element premix, 1.1% vitamin premix, 6.4% L-threonine, 5.7% sodium humate, 22.9% rice husk powder.
3. The layer chicken feed containing fermented brewer's grains according to claim 1, wherein the crude protein content in the soybean meal is 43wt%.
4. The layer chicken feed containing fermented brewer's grains according to claim 2, wherein the purity of said L-lysine sulfate is 70% and the purity of said choline chloride is 60%.
5. The layer feed containing fermented brewer's grains according to claim 1, wherein the raw materials comprise, by weight, 45-50% of corn, 8-9% of wheat, 18-19% of soybean meal, 5-6% of dried whole corn grains, 2.8-3.3% of sunflower seed meal, 9-9.5% of stone powder, 0.2-0.5% of vegetable oil, 2.0-2.5% of fermented brewer's grains, 1.2-1.8% of slurry-sprayed corn husks, 1-1.5% of calcium hydrogen phosphate, and 1-2% of additives.
6. The layer feed containing fermented brewer's grains according to claim 1, wherein its raw materials comprise 48.7% corn, 8.4% wheat, 18.5% soybean meal, 6.0% corn dried whole stillage, 3.0% sunflower seed meal, 9.2% stone powder, 0.3% vegetable oil, 2.0% fermented brewer's grains, 1.5% jet corn bran, 1% calcium hydrogen phosphate, 1.4% additives; wherein the ingredients of the additive consist of 17.9% L-lysine sulphate, 10.7% DL-methionine, 21.4% sodium chloride, 4.3% choline chloride, 1.1% phytase, 1.4% betaine, 7.1% mineral element premix, 1.1% vitamin premix, 6.4% L-threonine, 5.7% sodium humate, 22.9% rice hull flour.
7. The process for producing layer chicken feed containing fermented brewer's spent grain according to any one of claims 1-6, wherein it comprises the steps of:
1) Weighing the raw materials according to the weight ratio for later use;
2) Firstly, uniformly mixing calcium hydrophosphate and an additive, then adding other raw materials, and uniformly mixing to obtain the laying hen feed containing the fermented brewer's grains.
8. Use of the layer feed containing fermented brewer's spent grains according to any one of claims 1-6 for breeding layers.
CN202211040789.4A 2022-08-29 2022-08-29 Laying hen feed containing fermented brewer's grains and preparation method and application thereof Pending CN115316551A (en)

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CN103931908A (en) * 2013-01-23 2014-07-23 四川铁骑力士实业有限公司 Forage for improving later-period production performance of laying hens and eggshell quality
CN104782886A (en) * 2015-05-11 2015-07-22 唐人神集团股份有限公司 Formula feed for laying hens during egg-laying peak period and preparation method of formula feed
CN110150501A (en) * 2019-06-25 2019-08-23 安徽笑果农牧产业科技有限公司 A kind of high-yield egg chicken feed under heat stress
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Publication number Priority date Publication date Assignee Title
CN1759715A (en) * 2005-11-21 2006-04-19 中国食品发酵工业研究院 Direct placing on production of brewery mash and production method
CN103931908A (en) * 2013-01-23 2014-07-23 四川铁骑力士实业有限公司 Forage for improving later-period production performance of laying hens and eggshell quality
CN104782886A (en) * 2015-05-11 2015-07-22 唐人神集团股份有限公司 Formula feed for laying hens during egg-laying peak period and preparation method of formula feed
CN110150501A (en) * 2019-06-25 2019-08-23 安徽笑果农牧产业科技有限公司 A kind of high-yield egg chicken feed under heat stress
CN113229398A (en) * 2021-04-26 2021-08-10 华中农业大学 Compound feed additive based on brewer's grains fermentation and preparation method and use method thereof

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