CN115316248A - Culture method and application of Pinus thunbergii water culture seedlings - Google Patents
Culture method and application of Pinus thunbergii water culture seedlings Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/002—Culture media for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Cell Biology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Wood Science & Technology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to the technical field of plant cultivation, and discloses a cultivation method of water-cultured seedlings of black pines and application thereof. The method comprises the following steps: accelerating germination of the treated black pine seeds, selecting bud seedlings with good root systems to culture in an open tissue culture medium, and obtaining the real-time development state of the root systems; selecting bud seedlings with well developed root systems, transplanting the bud seedlings on a black pine seedling substrate to culture black pine seedlings, and inoculating blue-green algae on the black pine seedling substrate after culturing for a period of time; after suspension culture, removing part of the black pine seedling matrix to carry out hydroponic seedling root culture, and carrying out needle leaf formation and growth needle leaf formation culture on hydroponic seedlings by utilizing a black pine hydroponic liquid fertilizer. The invention fills the blank of the water culture technology of the Pinus thunbergii in China, does not use expensive culture solution for water culture seedlings, only uses tap water, and saves the seedling culture cost. The complex aeration device is not needed in the culture process, and the cost and the energy consumption of the seedling raising equipment are also saved.
Description
Technical Field
The invention belongs to the technical field of plant cultivation, and particularly relates to a culture method and application of a water culture seedling of Pinus thunbergii.
Background
Hydroponics, also known as hydroponics (solution culture), is a method of cultivating plants in a solution containing all or part of the nutrient elements. Hydroponic nursery stocks are common in the market at present, play a role in indoor decoration and removal of decoration pollutants, and have the characteristics of mobility, small occupied space and good management. Common hydroponic plants include herbs or vines such as Epipremnum aureum (Epipremnum aureum) and Chlorophytum comosum (Chlorophytum comosum), while the hydroponics of woody plants is less, and pine hydroponics is not reported successfully. The existing common hydroponic seedlings are generally not resistant to strong light and high temperature and have little effect on balcony greening. Pine is generally a strong positive tree species, so the pine seedling water culture can fill the gap and is suitable for balcony greening. Meanwhile, terpene secondary metabolites released in the growth process of the pine seedlings are also bacteriocins, so that the pine seedling water culture seedlings are not easy to suffer from plant diseases and insect pests, and meanwhile, the bacteriocins can reduce indoor flora and are beneficial to human health, so that the pine seedling water culture has wide market prospect. The pine seedling water culture also has great scientific research value, is suitable for developing visual dynamic research on the root system of the pine seedling, can carry out scientific research on the root system in a nondestructive mode, and is favorable for discussing the balance relationship between different nutrient solution components and plant growth. In addition, the cultivated moisture-resistant pine seedlings can also provide improved seeds for low-wetland afforestation.
Pinus thunbergii (Pinus thunbergii) is a plant of Pinaceae, pinus. Also known as white spruce, evergreen arbors, native to the southern coast of japan and korea. The black pine is a common garden tree species, can be used for greening roads, districts, squares and the like, has good greening effect, and has long disease resistance and life cycle. Pinus thunbergii is also commonly used in coastal windbreak forest, so the method has certain significance for the cultivation of good seeds with water and moisture resistance. At present, the culture research of the water culture seedling of the Pinus nigra has not been reported.
Although water culture of massoniana (p. Massoniana) has been tried, water culture of pine only stays in the experimental stage and cannot be used for production due to bottleneck problems of slow rooting speed, difficult bud induction and the like. The successful water culture of banksana (p. Banksiana) is reported abroad, but it adopts a complicated aeration device, and the device is not suitable for the water culture seedling culture of families.
The water molecule is composed of an O 2- And two H-groups formed by H-O bonds, which are not symmetrical, and which form a non-0 dipole moment (dipole moment) that imparts polarity to the water molecules, into which the various ions are dissolved, and which partially dissolve oxygen. The dissolved oxygen amount of water is 8.54 to 9.15mg/L, that is, 266.9 to 285.9. Mu. Mol/L, and such concentration does not restrict respiration because Km value of oxygen is less than 1. Mu. Mol/L in the reaction catalyzed by cytochrome C oxidase, so that it is possible to use water as a substrate for cultivating plants. The liquid phase diffusion rate of oxygen is very slow, 3 x 106 times slower than in air, which is the main limiting factor. Research shows that the root end respiration amount of the black pine root system is 2.62 mu L/(h.mg), and the water culture medium is difficult to meet the requirement. The elongation of the roots is affected by the oxygen content of the substrate, the rate of elongation of the roots is increased when the oxygen content is larger in a certain range, and the elongation of the hydroponic roots is difficult because the oxygen content of water is extremely small.
Through the above analysis, the problems and defects of the prior art are as follows:
(1) The success rate of water culture by directly using annual black pine seedlings in a nursery in the prior art is low, and the root systems of a plurality of tree species stop growing when the oxygen concentration is lower than 2 percent, so the root systems of the pine seedlings in the nursery can not adapt to the liquid matrix environment. The survival and development of the pine seedling root system often need a substrate with good air permeability, and the air permeability of the water culture substrate is difficult to meet the requirement.
(2) The prior art is suitable for the water culture of the black pine seedling method and has low transplanting survival rate.
(3) The components of the liquid fertilizer for hydroponics of the black pine in the prior art promote the formation and poor growth effect of needle leaves of hydroponics seedlings.
(4) The water culture of the Pinus thunbergii in the prior art is high in cost, and a complex ventilation device is needed in the culture process, so that the seedling raising equipment cost and the energy consumption are high.
Disclosure of Invention
In order to overcome the problems in the related art, the disclosed embodiment of the invention provides a culture method of a water culture seedling of black pine and application thereof.
The technical scheme is as follows: a cultivation method of water culture seedlings of Pinus thunbergii comprises the following steps:
s1, performing open tissue culture: accelerating germination of the treated Pinus thunbergii seeds, selecting bud seedlings with the growth quantity of more than 2cm for culturing in an open tissue culture medium, and obtaining the real-time development state of a root system;
s2, performing suspension culture: selecting the bud seedlings with well developed root systems in the step S1, namely, the main roots grow for more than 2cm, 1-2 lateral roots appear, and continuing to perform black pine seedling suspension culture on a matrix, wherein the matrix contains agar and cane sugar in a ratio of 6:1, applying activated carbon and naphthylacetic acid, inoculating blue-green algae on a black pine seedling substrate after culturing for 1 month, and improving the conductivity in a suspension liquid in the black pine seedling culture, wherein the conductivity before inoculation is 1403.7 +/-2.3 mu s/cm, and the conductivity after inoculation is 2403.7 +/-1.2 mu s/cm;
s3, carrying out water culture: after suspension culture in the step S2, removing part of the black pine seedling matrix to perform water culture seedling root culture, and performing leaf bud formation and conifer growth culture of the water culture seedling by using a liquid fertilizer for black pine water culture.
In one embodiment, in step S1, the processing method of the black pine seed includes: with KMnO 4 Treating with 0.2% for 2h, and then accelerating germination with warm water for 24 h. Firstly, culturing seeds in a filter paper culture dish to promote the seeds to germinate, and then transferring the seeds into a culture bottle.
In one embodiment, the flasks are sterilized with 70% ethanol for 30 seconds before being transferred, sterilized with NaClO2% solution for 15min, rinsed and transferred.
In one embodiment, in step S1, the open tissue culture medium is 3-9 g/L agar, 0.5% -1.5% activated carbon, 0.5-1.5 g sugar/L and 1-3 mg/LNAA. Preferably, the open tissue culture medium matrix is 6g/L agar, 1% active carbon, 1g sugar/L and 2mg/LNAA.
In one embodiment, in step S1, selecting a well rooted seedling to be cultured in an open tissue culture medium further includes:
placing the culture bottle for 24-48 h, placing the culture bottle into an illumination incubator, wherein the temperature is 20 ℃, the illumination intensity is 600-700lux, the illumination time is 14h/d, and the red light accounts for 13.6%.
In one embodiment, in step S2, blue-green algae is inoculated on the black pine seedling matrix culture medium for 1-2 months;
after blue-green algae is inoculated on the black pine seedling substrate, water is injected to suspend the black pine seedling substrate culture medium, a certain root system suspension space is maintained, and air bags around the root system are removed.
In one embodiment, in step S3, after 5-6 roots with the length of more than 2cm grow out at the lower part of the culture medium and the water interface, the culture medium is taken out together with seedlings, most of the culture medium is removed, and only part of the matrix below the root neck is reserved; transplanting the black pine subjected to suspension culture into a hydroponic bottle for further elongation of hydroponic seedling roots, and applying a liquid fertilizer to promote the formation and growth of needle leaves when 1-3 roots grow to more than 5 cm; 1-3ml each time.
The invention also aims to provide application of the culture method of the hydroponic culture seedlings of the Pinus thunbergii to culture the hydroponic seedlings of the scindapsus aureus.
The invention also aims to provide application of the culture method of the hydroponic seedlings of the black pines in the culture of the hydroponic seedlings of the chlorophytum comosum.
The invention also aims to provide application of the culture method of the water culture seedling of the Pinus thunbergii to culture the water culture seedling of the Pinus massoniana.
By combining all the technical schemes, the invention has the advantages and positive effects that:
first, aiming at the technical problems existing in the prior art and the difficulty in solving the problems, the technical problems to be solved by the technical scheme of the present invention are closely combined with the technical scheme to be protected and the results and data in the research and development process, and some creative technical effects brought after the problems are solved are analyzed in detail and deeply. The specific description is as follows:
the key point of the invention is that the pine seedling suitable for family water culture is cultured by the aquatic mutagenesis technology instead of developing a complex water culture device, and the pine seedling can be cultured and survived in water culture without a special device like a scindapsus aureus. The method mainly utilizes the plasticity of plants to gradually induce the plants to generate aquatic roots, so that pine seedlings are florified, and technical support is provided for the pine tree water culture seedlings to market.
Secondly, considering the technical solution as a whole or from the perspective of products, the technical effects and advantages of the technical solution to be protected by the present invention are specifically described as follows:
the invention provides a set of aquatic mutagenesis technology aiming at the characteristic that the black pine root system has higher oxygen demand and is difficult to be cultured in water, and the technology comprises three technical links of open tissue culture, suspension culture, water culture and the like, thereby forming black pine seedlings suitable for water culture.
The invention adopts an open tissue culture mode to develop the aquatic mutagenesis process of the black pine seedlings, and conducts a plurality of experiments on screening a culture formula suitable for open tissue culture to find out the optimal culture medium formula, namely 6g/L agar, 1% active carbon, 1g sugar/L and 2mg/LNAA, so that the transplanting survival rate reaches about 90%. The experiment is carried out in the illumination incubator, has set up the three horizontally orthogonal experiments of four factors totally, has set up the proportion of different agar and sucrose, and add and not add the contrast experiment of active carbon and NAA, and every group experiment is 10 times repeated, and the experiment is carried out under strict control environment, has guaranteed the uniformity of experiment environmental condition.
In the suspension culture stage, the invention provides a symbiotic system of blue-green algae and pine seedlings, which gradually improves the conductivity in the suspension and promotes the elongation growth of the roots of the pine seedlings. The experiment is carried out at the natural temperature and the illumination of an indoor balcony, the substrate moisture is gradually increased through periodic moisture management such as water injection and water drainage, a water root mutagenesis environment is formed, the contrast experiment of inoculated blue-green algae and non-inoculated blue-green algae is set on the basis, and each group of experiment is repeated for 10 times.
The invention provides a liquid fertilizer formula suitable for hydroponics of black pine, provides the optimal fertilizing time and fertilizing amount, and promotes the formation and growth of needles of hydroponic seedlings. The experiment compares three liquid fertilizer formula comparison experiments by taking tap water as a comparison, the liquid fertilizer formula consists of three parts, namely mineral nutrition, sugar and bactericide (NaClO), and each group of experiments is repeated for 10 times.
Third, as inventive supplementary proof of the claims of the present invention, there are several important aspects as follows:
(1) The expected income and commercial value after the technical scheme of the invention is converted are as follows: the method can cultivate the water culture seedlings of the Pinus thunbergii suitable for greening the balcony of the family, fills the blank of the market in the field, has larger expected income and has higher commercial value.
(2) The technical scheme of the invention fills the technical blank in the industry at home and abroad: the invention fills the blank of the water culture technology of the Pinus thunbergii in China, does not use expensive culture solution for water culture seedlings, only uses tap water, and saves the seedling culture cost. The complex aeration device is not needed in the culture process, and the cost and the energy consumption of the seedling raising equipment are also saved.
(3) The technical scheme of the invention solves the technical problems which are always desired to be solved but are not successful: the existing common hydroponic seedlings are generally not resistant to strong light and high temperature, and have little effect on balcony greening with strong light and high temperature. The black pine is a strong positive tree species, and the water culture seedling can fill the blank and is more suitable for balcony greening. The terpene secondary metabolites released by the black pine hydroponic seedlings can reduce indoor flora and are beneficial to human health.
(4) The technical scheme of the invention overcomes the technical prejudice that: the pine is considered to be an aerobic tree species in the past and cannot grow in a water environment, so the pine seedling water culture technology is slow in progress.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present disclosure and together with the description, serve to explain the principles of the disclosure.
FIG. 1 is a flow chart of a method for cultivating hydroponic seedlings of Pinus thunbergii according to an embodiment of the present invention;
FIG. 2 (a) is a schematic diagram of a length of a sprout in a culture dish suitable for transplantation according to an embodiment of the present invention;
FIG. 2 (b) is a schematic diagram showing the growth amount of sprouts and the phenomenon of automatic decapping after 8 days of transplantation, according to an embodiment of the present invention;
FIG. 3 (a) is a diagram of a pine seedling as it is being incorporated into a suspension system as provided by an embodiment of the present invention;
FIG. 3 (b) is a picture of a layer of blue-green algae covered by the pine seedling matrix after 1-2 months provided by the embodiment of the invention;
FIG. 4 (a) is a plan view of a suspension system provided by an embodiment of the present invention;
FIG. 4 (b) is a bottom view of a suspension system provided by an embodiment of the present invention;
FIG. 5 (a) is a water-cultured seedling map of Pinus thunbergii provided by the embodiment of the invention;
FIG. 5 (b) is a diagram of a water root provided by an embodiment of the present invention.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, but rather should be construed as broadly as the present invention is capable of modification in various respects, all without departing from the spirit and scope of the present invention.
1. Illustrative examples are illustrated:
fig. 1 shows that the culture method of the water culture seedling of the Pinus thunbergii provided by the embodiment of the invention comprises the following steps:
s101, performing open tissue culture: accelerating germination of the treated Pinus thunbergii seeds, selecting bud seedlings with the growth quantity of more than 2cm for culturing in an open tissue culture medium, and obtaining the real-time development state of a root system;
s102, performing suspension culture: selecting the bud seedlings with well developed root systems in the step S1, namely, the main roots grow for more than 2cm, 1-2 lateral roots appear, and continuing to perform black pine seedling suspension culture on a matrix, wherein the matrix contains agar and cane sugar in a ratio of 6:1, applying activated carbon and naphthylacetic acid, inoculating blue-green algae on a black pine seedling substrate after culturing for 1 month, and improving the conductivity in a suspension liquid in the black pine seedling culture, wherein the conductivity before inoculation is 1403.7 +/-2.3 mu s/cm, and the conductivity after inoculation is 2403.7 +/-1.2 mu s/cm;
s103, carrying out water culture: after the suspension culture in the step S102, part of the black pine seedling matrix is removed to perform water culture seedling root culture, and leaf bud formation and conifer growth culture of the water culture seedling are performed by utilizing a liquid fertilizer for black pine water culture.
As a preferred embodiment of the present invention, in step S101, the method for treating the black pine seed includes: with KMnO 4 Treating with 0.2% for 2h, and then accelerating germination with warm water for 24 h. Seeds were first cultured in a filter paper petri dish to promote germination, and then transferred to a culture flask.
As a preferred embodiment of the invention, the culture flask is sterilized by 70% alcohol for 30s before being transferred into the culture flask, then sterilized by NaClO2% solution for 15min, washed and transferred into the culture flask.
As a preferred embodiment of the present invention, in step S101, the open tissue culture medium comprises agar 3-9 g/L, activated carbon 0.5-1.5%, sugar 0.5-1.5 g/L, and LNAA 1-3 mg/L. Preferably, the open tissue culture medium is 6g/L agar, 1% active carbon, 1g sugar/L and 2mg/L NAA.
As a preferred embodiment of the present invention, in step S101, selecting a rooted seedling to be cultured in an open tissue culture medium further includes:
placing the culture bottle for 24-48 h, placing the culture bottle into an illumination incubator, wherein the temperature is 20 ℃, the illumination intensity is 600-700lux, the illumination time is 14h/d, and the red light accounts for 13.6%.
As a preferred embodiment of the invention, in step S102, blue-green algae is inoculated on the black pine seedling substrate culture medium for 1-2 months;
after blue-green algae is inoculated on the black pine seedling substrate, water is injected to suspend the black pine seedling substrate culture medium, a certain root system suspension space is maintained, and air bags around the root system are removed.
As a preferred embodiment of the invention, in step S103, after 5-6 roots with a length of more than 2cm grow out at the lower part of the culture medium and the water interface, the culture medium is taken out together with seedlings, most of the culture medium is removed, and only part of the matrix below the root neck is reserved; transplanting the suspension cultured Pinus thunbergii into a hydroponic bottle for further extending the hydroponic seedling roots, and applying a liquid fertilizer to promote the formation and growth of needle leaves when 1-3 roots grow to more than 5 cm; 1-3ml each time.
2. The technical scheme of the invention is further described by combining the attached drawings and experimental effects.
The method for cultivating the water culture seedlings of the Pinus thunbergii provided by the embodiment of the invention comprises the following steps:
(1) Open tissue culture stage
Firstly, culturing bud seedlings by utilizing black pine seeds, and selecting a root system from the bud seedlings to enter a second stage. KMnO for seeds 4 Treating with 0.2% for 2h, and then accelerating germination with warm water for 24 h. Firstly, seeds are cultured in a filter paper culture dish to promote the germination, and the seeds can be transferred into a culture bottle when the seeds grow to be more than about 2 cm.
The cells were sterilized with 70% ethanol for 30s before transfer, then sterilized with NaClO2% solution for 15min, rinsed and transferred to a culture flask. Through contrast screening, the best culture medium, namely 6g/L agar, 1% active carbon, 1g sugar/L and 2mg/LNAA, is selected, and the formula can enable the transplanting survival rate to reach about 90% under the open type tissue culture condition. And (4) screwing the bottle cap, placing for 24-48 h, loosening the bottle cap, and placing into an illumination incubator. The temperature is 20 ℃, the illumination intensity is 600-700lux, the illumination time is 14h/d, and the red light accounts for 13.6 percent.
Among them, the growth process (Bar =1 cm) provided by the present invention was transferred from a culture dish (fig. 2 (a)) to a tissue culture flask (fig. 2 (b)). FIG. 2 (a) shows the length of a seedling suitable for transplantation, and FIG. 2 (b) shows the growth amount of a seedling and the phenomenon of automatic decapping 8 days after transplantation.
After 1 week, the seedlings grow cotyledons and are automatically uncapped (namely, the seed coats are removed, figure 2 (b)) in succession, and the seed coats are taken out by using tweezers. And (5) continuing to culture. Observing the development condition of the root system, selecting a better one, and entering the next culture stage.
(2) Suspension culture phase
Removing the bottle cap, opening the culture bottle, and continuously culturing at the natural temperature and illumination of the indoor balcony. Injecting water (tap water) to submerge the culture medium for 2-3mm every morning, and pouring off at night to keep the matrix ventilation at night.
In 1-2 months, blue-green algae (pine seedlings just brought into the suspension system (figure 3 (a)) and a layer of blue-green algae (figure 3 (b)) covered on the pine seedling matrix after 1-2 months) are inoculated on the culture medium, the separation of the culture medium and the culture bottle is accelerated by the blue-green algae, and then the suspension culture stage is carried out.
Then water is injected to suspend the culture medium, the suspension height is about 5mm (the suspension system is in a plan view (figure 4 (a)) and in a bottom view (figure 4 (b)), wherein (Bar =1 cm).
(3) Hydroponic culture stage
When 5-6 roots with the length of more than 2cm grow out at the lower part of the culture medium and the water interface, taking out the culture medium and seedlings together. Most of the medium was carefully removed, leaving only a small portion of the matrix below the root neck. The suspension cultured black pine is transferred into a hydroponic bottle (black pine hydroponic seedling (fig. 5 (a)) and water roots (fig. 5 (b), bar =5 mm). The culture solution in the hydroponic bottle is used with ordinary tap water, the hydroponic seedling is cultured with tap water for the first time without adding a nutrient solution, so that further elongation of the hydroponic seedling roots can be promoted, and when 1-3 roots grow to more than 5cm, a liquid fertilizer can be applied in order to promote needle leaf formation or growth.A liquid fertilizer formula is shown in table 1. 1-3ml is applied each time.
TABLE 1 optimized liquid fertilizer formulation
In the above embodiments, the descriptions of the respective embodiments have respective emphasis, and reference may be made to the related descriptions of other embodiments for parts that are not described or illustrated in a certain embodiment.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention, and the scope of the present invention should not be limited thereto, and any modifications, equivalents and improvements made by those skilled in the art within the technical scope of the present invention as disclosed in the present invention should be covered thereby.
Claims (10)
1. The cultivation method of the water culture seedlings of the black pines is characterized by comprising the following steps of:
s1, performing open tissue culture: accelerating germination of the treated black pine seeds, selecting bud seedlings with good root systems, and culturing the bud seedlings in an open tissue culture medium to obtain a real-time development state of the root systems;
s2, performing suspension culture: selecting the bud seedlings with well developed root systems in the step S1, inoculating blue-green algae on the black pine seedling substrate, and improving the internal conductivity of the suspension in the black pine seedling culture;
s3, carrying out water culture: after suspension culture in the step S2, removing part of the black pine seedling matrix to perform water culture seedling root culture, and performing leaf bud formation and conifer growth culture of the water culture seedling by using a liquid fertilizer for black pine water culture.
2. The method for cultivating hydroponic seedlings of Pinus thunbergii as claimed in claim 1, wherein in step S1, the method for treating Pinus thunbergii seeds comprises: with KMnO 4 Treating with 0.2% for 2h, accelerating germination with warm water for 24h, culturing seeds in a filter paper culture dish to promote germination, and transferring into a culture bottle.
3. The method for cultivating hydroponic seedlings of Pinus nigra as claimed in claim 2, wherein the cultivation bottle is sterilized with 70% ethanol for 30s before being transferred into the cultivation bottle, sterilized with NaClO2% solution for 15min, washed and transferred into the cultivation bottle.
4. The culture method of the Pinus thunbergii hydroponic seedlings according to claim 1, wherein in the step S1, the open type tissue culture medium comprises 3g/L to 9g/L of agar, 0.5 percent to 1.5 percent of activated carbon, 0.5g to 1.5g of sugar/L and 1mg/L to 3mg/LNAA.
5. The method for cultivating hydroponic seedlings of Pinus thunbergii according to claim 1, wherein in step S1, selecting the rooted seedlings to be cultivated in an open tissue culture medium further comprises: the culture bottle is placed for 24 h-48 h, and is placed in an illumination incubator, the temperature is 20 ℃, the illumination intensity is 600lux-700lux, the illumination time is 14h/d, and the red light accounts for 13.6%.
6. The method for cultivating Pinus thunbergii hydroponic seedlings according to claim 1, wherein in step S1, sprouts with a growth amount of more than 2cm are selected and cultured in an open tissue culture medium.
7. The culture method of the hydroponic seedling of the Pinus thunbergii according to claim 1, wherein in the step S2, blue-green algae is inoculated on the Pinus thunbergii seedling matrix culture medium for 1-2 months;
after blue-green algae is inoculated on the black pine seedling substrate, water is injected to suspend the black pine seedling substrate culture medium, a certain root system suspension space is maintained, and air bags around the root system are removed.
8. The method for cultivating hydroponic seedlings of Pinus nigra as claimed in claim 1, wherein in step S2, the seedlings with the growth amount of more than 2cm in step S1 are selected and have 1-2 lateral roots, the suspension culture of the Pinus nigra seedlings is continued on the substrate, and after 1 month of culture, blue-green algae is inoculated on the Pinus nigra seedling substrate.
9. The method for cultivating hydroponic seedlings of Pinus thunbergii according to claim 1, wherein in step S3, after 5-6 roots with a length of 2cm or more grow out at the interface between the lower part of the culture medium and the water, the culture medium is taken out together with the seedlings, most of the culture medium is removed, and only the part of the matrix below the root neck is retained; transplanting the suspension cultured Pinus thunbergii into a hydroponic bottle for further extending the hydroponic seedling roots, and applying a liquid fertilizer to promote the formation and growth of needle leaves when 1-3 roots grow to more than 5 cm; 1-3ml each time.
10. An application of the hydroponic culture method of Pinus thunbergii as claimed in any one of claims 1 to 7 in hydroponic culture of scindapsus aureus, chlorophytum comosum and Pinus massoniana.
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