CN115300596A - Microecological extract for preventing and treating thyroid nodules and preparation method thereof - Google Patents

Microecological extract for preventing and treating thyroid nodules and preparation method thereof Download PDF

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Publication number
CN115300596A
CN115300596A CN202210945538.4A CN202210945538A CN115300596A CN 115300596 A CN115300596 A CN 115300596A CN 202210945538 A CN202210945538 A CN 202210945538A CN 115300596 A CN115300596 A CN 115300596A
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parts
microecological
extract
lactobacillus
radix
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郝冰娜
蔡睿
赵乙木
赵环
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Shenzhen New Target Biotechnology Co ltd
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Shenzhen New Target Biotechnology Co ltd
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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    • A61K31/20—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
    • A61K31/202—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids having three or more double bonds, e.g. linolenic
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Abstract

The invention discloses a microecological extract for preventing and treating thyroid nodules, which is prepared from the following components in parts by weight: 10-20 parts of ternate buttercup root, 20-30 parts of selfheal, 10-20 parts of curcuma zedoary, 10-20 parts of thunberg fritillary bulb, 10-20 parts of radix bupleuri, 10-20 parts of radix curcumae, 10-20 parts of pericarpium citri reticulatae viride, 30-50 parts of raw oyster, 10-20 parts of pinellia ternate, 20-40 parts of astragalus membranaceus, 10-20 parts of dried orange peel, 5-15 parts of fructus aurantii, 10-15 parts of poria cocos, 8-12 parts of rhizoma cyperi, 8-12 parts of platycodon grandiflorum, 8-12 parts of liquorice, 8-12 parts of elecampane, 8-12 parts of allium macrostemon, 8-12 parts of radix ophiopogonis, 8-12 parts of cowherb seed, 8-12 parts of garden burnet, 8-12 parts of radix glehniae, 8-12 parts of herb tea, 8-12 parts of perilla seed, 8-12 parts of uncaria, 8-12 parts of polygala tenuifolia, 0.1 part of OMEGA-3, 0.001 part of B-carotene, 0.05 part of quercetin and 0.00005 part of organic selenium. The invention also discloses a preparation method of the microecological extract for preventing and treating thyroid nodules. The invention has the advantages of higher extraction efficiency, low toxic component, high human body absorptivity, good treatment effect, obvious treatment effect and less side effect.

Description

Microecological extract for preventing and treating thyroid nodules and preparation method thereof
Technical Field
The invention belongs to the technical field of microecological extracts, and particularly relates to a microecological extract for preventing and treating thyroid nodules and a preparation method thereof.
Background
Thyroid nodule is a mass with one or more abnormal tissue structures in the thyroid gland caused by various reasons, is a scattered pathological change caused by the abnormal growth of thyroid cells in local parts, can move up and down along with swallowing action and the thyroid gland, and is a common disease and frequently encountered disease. Although thyroid nodules do not pose a life safety threat directly, they stress surrounding tissues: oppression on the recurrent laryngeal nerve leads to paralysis of the vocal cords and hoarseness; oppression on the trachea causes chest distress and dyspnea; oppresses the esophagus, causing the pharynx to have a feeling of obstruction and even dysphagia; the blood vessels are pressed, which causes difficult blood backflow and makes the face of the patient bluish purple and swollen, and the superficial veins of the neck and chest dilate. And thyroid nodule has cancerization tendency, can cause endocrine disturbance, and can also cause muscular atrophy, relaxation and weakness to cause thyroid crisis and the like.
Thyroid nodules have 4 cases: 1. the single node is substantive, the single thyroid node of the nuclide scanning thermojunction is single, and the canceration probability is low. The cold nodule usually requires surgery. The single node with rapid development and hard texture may be malignant and should be operated early. 2. The difference in the incidence of single-shot multiple-cancerous changes is not large, and it is considered that the cytological diagnosis of malignant or suspected malignant patients should be operated. 3. The palpable small nodules, which are the most common at present, are the palpable thyroid nodules found small by physical examination, ultrasound, CT, magnetic resonance. If the depth is less than 1.5cm, the puncture is needed only if the depth is more than 1.5cm, and the judgment is made according to pathological examination. 4. Thyroid cancer is easy to occur when radiation treatment is carried out on a radiation nodule and the head and the neck, and if the nodule appears on the thyroid gland 5-30 years after radiation, the thyroid gland should be punctured immediately for pathological examination.
At present, the clinical treatment measures for thyroid nodules include TSH inhibition treatment, 131-I treatment, percutaneous absolute alcohol injection (PEI) under ultrasonic guidance, percutaneous laser ablation, radio frequency ablation, surgical operations and the like, but still some thyroid nodule patients cannot be effectively controlled, and the side effects are large. Although the traditional Chinese herbal medicines have small side effect, the traditional Chinese herbal medicines have more impurities, are inconvenient to take and have low extraction efficiency, and the curative effect and the application are influenced.
Therefore, the research on the microecological extract for preventing and treating thyroid nodules and the preparation method thereof are particularly necessary.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide the microecological extract for preventing and treating thyroid nodules and the preparation method thereof.
One of the purposes of the invention is realized by adopting the following technical scheme:
a microecological extract for preventing and treating thyroid nodule is prepared from the following components: radix ranunculi ternati, selfheal, curcuma zedoary, thunberg fritillary bulb, radix bupleuri, radix curcumae, pericarpium citri reticulatae viride, raw oyster, pinellia ternate, astragalus membranaceus, pericarpium citri reticulatae, fructus aurantii, poria cocos, rhizoma cyperi, platycodon grandiflorum, liquorice, elecampane, allium macrostemon, radix ophiopogonis, cowherb seed, garden burnet, radix glehniae, royal tea, perilla seed, uncaria, polygala tenuifolia, OMEGA-3, B-carotene, quercetin and organic selenium.
In the invention, a further preferable scheme is that the adhesive is prepared from the following components in parts by weight: 10-20 parts of ternate buttercup root, 20-30 parts of selfheal, 10-20 parts of curcuma zedoary, 10-20 parts of thunberg fritillary bulb, 10-20 parts of radix bupleuri, 10-20 parts of radix curcumae, 10-20 parts of pericarpium citri reticulatae viride, 30-50 parts of raw oyster, 10-20 parts of pinellia ternate, 20-40 parts of astragalus membranaceus, 10-20 parts of dried orange peel, 5-15 parts of fructus aurantii, 10-15 parts of poria cocos, 8-12 parts of rhizoma cyperi, 8-12 parts of platycodon grandiflorum, 8-12 parts of liquorice, 8-12 parts of elecampane, 8-12 parts of allium macrostemon, 8-12 parts of radix ophiopogonis, 8-12 parts of cowherb seed, 8-12 parts of garden burnet, 8-12 parts of radix glehniae, 8-12 parts of herb tea, 8-12 parts of perilla seed, 8-12 parts of uncaria, 8-12 parts of polygala tenuifolia, 0.1 part of OMEGA-3, 0.001 part of B-carotene, 0.05 part of quercetin and 0.00005 part of organic selenium.
In the invention, a further preferable scheme is that the adhesive is prepared from the following components in parts by weight: 15 parts of radix ranunculi ternati, 25 parts of selfheal, 15 parts of curcuma zedoary, 15 parts of thunberg fritillary bulb, 15 parts of radix bupleuri, 15 parts of radix curcumae, 15 parts of pericarpium citri reticulatae viride, 40 parts of raw oyster, 15 parts of pinellia ternate, 30 parts of radix astragali, 15 parts of pericarpium citri reticulatae, 10 parts of fructus aurantii, 12 parts of poria cocos, 10 parts of rhizoma cyperi, 10 parts of platycodon grandiflorum, 10 parts of liquorice, 10 parts of elecampane, 10 parts of allium macrostemon, 10 parts of radix ophiopogonis, 10 parts of cowherb seed, 10 parts of garden burnet, 10 parts of radix glehniae, 10 parts of king tea, 10 parts of perilla seed, 10 parts of uncaria, 10 parts of polygala tenuifolia, 0.1 part of OMEGA-3, 0.001 part of B-carotene, 0.05 part of quercetin and 0.00005 part of organic selenium.
A preparation method of a microecological extract for preventing and treating thyroid nodules comprises the following steps:
step 1) taking the components according to a formula, mixing, sterilizing and crushing to obtain a raw material mixture, and adding pepsin into the raw material mixture for enzymolysis treatment to obtain an enzymolysis raw material mixture;
step 2) carrying out microbial ecological extraction treatment on the raw material mixture obtained in the step 1 by using microorganisms, wherein the extraction treatment specifically comprises carrying out primary fermentation on the raw material mixture subjected to enzymolysis under an acidic condition, wherein the primary fermentation temperature is 35-38 ℃, the primary fermentation time is 15-25 days, and the pH value of the primary fermentation is 2-7; performing static fermentation and chelation on the raw material mixture subjected to acid fermentation at normal temperature, wherein the static fermentation and chelation time is 10-25 days, and the pH value is 2-5; then filtering, removing filter residues, and collecting the micro-ecological extracting solution;
and 3) sterilizing the microecological extract, and then curing to obtain the microecological extract.
In the present invention, it is further preferable that the method further comprises, after the step 3, a step 4: adding food acceptable auxiliary materials into the microecological extract obtained in the step 3.
In the present invention, it is further preferable that step 4 is followed by step 5: heating the microecological extract treated in the step (4) until the auxiliary materials are dissolved, and then sterilizing; the adjuvant is soluble in water.
In the present invention, it is further preferred that the amount of pepsin in step 1 is 0.5 to 3% by weight of the raw material mixture.
In the present invention, it is further preferable that the aging treatment in the step 3 specifically comprises: and (3) placing the microecological extracting solution into a sterilized curing barrel for sealed curing preservation at room temperature.
In the present invention, it is further preferable that the microorganism obtained by the primary fermentation in step 2 comprises the following strains in parts by weight based on the total weight of the components: 1 part of lactobacillus casei, 1 part of lactobacillus rhamnosus, 1 part of lactobacillus acidophilus, 1 part of bifidobacterium adolescentis, 1 part of bifidobacterium longum, 0.1 part of lactobacillus reuteri, 0.5 part of lactobacillus crispatus, 0.5 part of lactobacillus paracasei subspecies paracasei, 0.5 part of lactobacillus helveticus, 0.5 part of lactobacillus fermentum, 0.5 part of streptococcus thermophilus, 0.5 part of lactobacillus gasseri, 0.5 part of lactobacillus delbrueckii subspecies bulgaricus, 0.4 part of lactobacillus plantarum, 0.1 part of bifidobacterium lactis, 0.1 part of bifidobacterium bifidum, 0.1 part of bifidobacterium infantis, 0.1 part of bifidobacterium breve, 0.1 part of lactobacillus johnsonii, 0.1 part of lactobacillus salivarius, 0.1 part of lactobacillus subspecies lactis, 0.1 part of lactococcus lactis subspecies cremorium, 0.1 part of lactococcus lactis diacetyl subspecies lactis, and 0.1 part of leuconostoc mesenteroides.
In the present invention, it is further preferable that the microorganism in the step 2 further includes a microorganism for degrading an organophosphorus pesticide.
Compared with the prior art, the invention has the beneficial effects that:
the microecological extract consists of ternate buttercup root, selfheal, curcuma zedoary, thunberg fritillary bulb, radix bupleuri, radix curcumae, pericarpium citri reticulatae viride, raw oyster, pinellia ternate, astragalus membranaceus, dried tangerine peel, fructus aurantii, poria cocos, rhizoma cyperi, platycodon grandiflorum, liquorice, elecampane, allium macrostemon, radix ophiopogonis, cowherb seed, garden burnet, radix glehniae, king tea, perilla seed, uncaria, polygala tenuifolia, OMEGA-3, B-carotene, quercetin and organic selenium. Among the components, radix ranunculi ternati, selfheal, allium macrostemon and pinellia ternate can remove blood stasis, eliminate stasis and remove scrofula and subcutaneous nodule; radix bupleuri, radix curcumae, fructus aurantii, pericarpium citri reticulatae viride, elecampane, rhizoma cyperi and cowherb seed, and uncaria can calm the liver, activate stagnation and relieve depression; oyster and astragalus root. Polygala root, radix ophiopogonis, ophiopogon root, OMEGA-3, B-carotene, quercetin and organic selenium, can eliminate inflammatory factors, resist free radicals and oxidation, are fermented and extracted to generate postbiotic and synbiotic factors, increase nutrition, regulate metabolism and improve immunity, are coordinated, supplemented and synergized, so that the microecological extract has the comprehensive treatment effect of treating thyroid nodules, improving microenvironment of thyroid tissues and conditioning metabolic imbalance caused by the thyroid nodules, and has the advantages of remarkable treatment effect and less side effect. In addition, results of network molecular pharmacology analysis demonstrated that the prescription-related compound can intervene in the risk of tumorigenesis of a nodule by modulating tumor-related genes (AKT 1; AKT2; AURKA; CDK2; CDK7; FGFR1; FNTA; HSP90AA1; LTA4H; NFKB2; PIK3CG; PTPN6; RPS6KB1; SGK1; VDAC2; AKT1; AKT2; CCND1; COPS5; CXCR4; CYP19A1; DNMT3B; EGFR; ESR1; ESR2: ESRRA: FNTA: FOS: HSP90AA1: LHCGR: MAP2K1: MDM 2K5: MMP2: NCOA 3. The metabolism is regulated by regulating related genes (ABCC 8; ACACACB; ADRB3; CEBPB; CPT1A; CPT1B; CRHR1; CRHR2; DPP4; ESRRA; GHRL; HCRT; HSD11B1; MAPK9; MC1R; MC3R; MC4R; MLNR; NPY2R; NPY5R; NR1I3; PPARA; PPARD; PPARG; THRB). Depressive states are improved by regulation of (ADORA 2A; ADORA3; ADRB2; CREB1; CRHR1; DRD2; GLRA1; HTR1A; HTR2A; IDO1; MAOA; NR1I2; NTSR1; OPRL1; SCNN1A; SLC52A2; SLC6A2; SMPD4; TACR 2) -associated genes. The risk of cardiovascular and cerebrovascular diseases caused by thyroid nodules is interfered by regulating and controlling (ACE; ALOX15; ALOX15B; F2R; HMOX1; KCNA5; PDE3A; PDE5A; ROCK1, ABCA1; ACE2; ADRB1; AGTR1; BDKRB2; CACNA1B; CACNA1G; CACNA1I; CDK2; CES1; CYBB; ECE1; EDN1; EPHX2; ESR1; ESR2; F10; HMGCR; HMOX1; MTTP; NCF1; NOS3; PDE1A; PDE1B; PDE1C; PTGS1; RELA; related genes). The probiotic composition has high content of active beneficial ingredients and increases the system conditioning function, and the active ingredients of the traditional Chinese medicines, such as crude polysaccharide, polyphenol, saponin, total flavone, triterpenoid quinone compounds, total polypeptide and the like, can be increased by 5-30 times compared with the active ingredients of various traditional Chinese medicines in the process of utilizing the traditional Chinese medicine ingredients as nutrient substances for growth, reproduction and metabolism.
2. The traditional Chinese medicine probiotics fermentation combination degrades macromolecular substances which cannot be absorbed by human bodies into active micromolecules which can be absorbed by human bodies, and the micromolecule substances can be rapidly absorbed into blood through alimentary canals and can be combined with cell proteins of the organisms through blood brain barriers, so that the absorption and utilization of the organisms to effective components are improved, and the absorption rate can be improved by times
3. In the microecological combined fermentation and extraction process, except for the increase of small molecular effective components in the conventional effective traditional Chinese medicine, microecological active metabolites such as vitamins, enzymes, proteins, organic acids, short-chain fatty acids, cell polysaccharides and the like are generated, and also immune active substances such as lipoteichoic acid, teichoic acid, peptidoglycan, cell surface protein, cell membrane protein, extracellular polysaccharide and the like are generated. The nutrition metabolism balance regulating function of the traditional Chinese medicine compound is increased, and the conditioning effects in multiple aspects such as anti-inflammation, intestinal barrier maintenance, immune metabolism regulation, cardiovascular diseases, antibiosis, liver protection, antioxidation and the like are increased. The Chinese medicinal composition really realizes the acceleration of the curative effect of the Chinese medicament, reduces the toxicity and enhances the effect, improves the mouthfeel, is favorable for releasing the active ingredients of the Chinese medicament from cells, and can extract and utilize the active ingredients of the Chinese medicament to the maximum extent; the probiotics can change own metabolic pathway in the special environment of the traditional Chinese medicine, so that more abundant metazoan and synbiotics effective metabolites are generated, and more active ingredients are formed;
4. the probiotic composition disclosed by the invention can degrade toxic components in the traditional Chinese medicine, such as organophosphorus pesticide, heavy metal, plasticizer and the like, or chelate toxic active ingredients in the traditional Chinese medicine, so that the toxic and side effects of the traditional Chinese medicine are reduced;
5. the established process avoids the damage of active ingredients, saves the resources of the traditional Chinese medicine, adopts the micro-ecological extraction process, is beneficial to the release of all micromolecule active ingredients, is not limited by the extraction conditions of fat solubility, alcohol solubility, water solubility and the like, and simultaneously avoids the damage of the traditional Chinese medicine to the active ingredients in the processes of decoction, boiling, steaming, refining and the like; and greatly saves the resources of the traditional Chinese medicine.
In addition, the preparation method is easy to operate and convenient for industrial production.
Detailed Description
The present invention is further described below with reference to specific embodiments, and it should be noted that, without conflict, any combination between the embodiments described below or between the technical features may form a new embodiment. Except as specifically noted, the materials and equipment used in this example are commercially available.
A microecological extract for preventing and treating thyroid nodules is prepared from the following components in parts by weight: 10-20 parts of radix ranunculi ternati, 20-30 parts of selfheal, 10-20 parts of curcuma zedoary, 10-20 parts of thunberg fritillary bulb, 10-20 parts of radix bupleuri, 10-20 parts of radix curcumae, 10-20 parts of pericarpium citri reticulatae viride, 30-50 parts of raw oyster, 10-20 parts of pinellia ternate, 20-40 parts of radix astragali, 10-20 parts of pericarpium citri reticulatae, 5-15 parts of fructus aurantii, 10-15 parts of poria cocos, 8-12 parts of rhizoma cyperi, 8-12 parts of platycodon grandiflorum, 8-12 parts of liquorice, 8-12 parts of elecampane, 8-12 parts of allium macrostemon, 8-12 parts of radix ophiopogonis, 8-12 parts of cowherb seed, 8-12 parts of garden burnet, 8-12 parts of radix glehniae king, 8-12 parts of herb tea, 8-12 parts of perilla seed, 8-12 parts of uncaria, 8-12 parts of polygala tenuifolia, 0.1 part of OMEGA-3, 0.001 part of B-carotene, 0.05 part of quercetin and 0.00005 part of organic selenium.
As a preferred embodiment, the microecological extract is prepared from the following components in parts by weight: 15 parts of radix ranunculi ternati, 25 parts of selfheal, 15 parts of curcuma zedoary, 15 parts of thunberg fritillary bulb, 15 parts of radix bupleuri, 15 parts of radix curcumae, 15 parts of pericarpium citri reticulatae viride, 40 parts of raw oyster, 15 parts of pinellia ternate, 30 parts of radix astragali, 15 parts of pericarpium citri reticulatae, 10 parts of fructus aurantii, 12 parts of poria cocos, 10 parts of rhizoma cyperi, 10 parts of platycodon grandiflorum, 10 parts of liquorice, 10 parts of elecampane, 10 parts of allium macrostemon, 10 parts of radix ophiopogonis, 10 parts of cowherb seed, 10 parts of garden burnet, 10 parts of radix glehniae, 10 parts of king tea, 10 parts of perilla seed, 10 parts of uncaria, 10 parts of polygala tenuifolia, 0.1 part of OMEGA-3, 0.001 part of B-carotene, 0.05 part of quercetin and 0.00005 part of organic selenium.
A preparation method of a microecological extract for preventing and treating thyroid nodules comprises the following steps:
step 1) taking the components according to a formula, mixing, sterilizing and crushing to obtain a raw material mixture, and adding pepsin into the raw material mixture for enzymolysis treatment to obtain an enzymolysis raw material mixture;
step 2) carrying out microbial ecological extraction treatment on the raw material mixture obtained in the step 1 by using microorganisms, wherein the extraction treatment specifically comprises the step of carrying out primary fermentation on the raw material mixture subjected to enzymolysis under an acidic condition, wherein the primary fermentation temperature is 35-38 ℃, the primary fermentation time is 15-25 days, and the primary fermentation pH value is 2-7; performing static fermentation and chelation on the raw material mixture subjected to acid fermentation at normal temperature, wherein the static fermentation and chelation time is 10-25 days, and the pH value is 2-5; then, filtering, removing filter residues, and collecting the micro-ecological extracting solution;
and 3) sterilizing the microecological extract, and then curing to obtain the microecological extract.
In the present invention, it is further preferable that the aging treatment in the step 3 specifically comprises: and (3) placing the microecological extracting solution into a sterilized curing barrel for sealed curing storage, wherein the temperature for sealed curing storage is room temperature.
In the present invention, it is further preferable that the microorganism in step 2 further includes a microorganism for degrading an organophosphorus pesticide.
As a preferred embodiment, step 4 is further included after step 3: adding food acceptable auxiliary materials into the microecological extract obtained in the step 3. In order to be more convenient for consumers to eat, auxiliary materials acceptable for food can be added into the microecological extract obtained in the step 3 to prepare different product dosage forms so as to enrich the mouthfeel of the product and facilitate storage and transportation, and specifically, a sweetening agent and the like can be added according to the needs or the preference of consumers, or the microecological extract is further prepared into oral liquid, tablets and the like; for example, step 4 is followed by step 5: heating the microecological extract treated in the step (4) until the auxiliary materials are dissolved, and then sterilizing; the adjuvants are soluble in water, and can be sweetener (such as granulated sugar, sucralose, mogroside), etc.
The sterilization treatment is not particularly limited, and for the sake of facilitating industrial operation, ozone sterilization treatment may be employed for 4 to 8min.
For filtering the microorganism extract, a 100-mesh sieve can be selected for filtering; for the sterilization treatment of the microorganism extract in step 3, pasteurization can be carried out, such as sterilization at 85 + -5 deg.C for 30-35 min; after the microbial extracting solution is treated in the steps 4 and 5, the treatment such as sterilization, filling, packaging and the like can be continuously carried out for facilitating the storage and transportation of the product; in order to inspect and monitor the quality of the product, the physical and chemical indexes of the product, such as total acid, crude polysaccharide, free amino acid, short-chain fatty acid, microorganism and the like, can be detected.
In order to enhance the flavor of the microbial extract of the present invention, a ripening treatment may be performed, and the ripening treatment in the step 3 is specifically: placing the micro-ecological extracting solution into a sterilized curing barrel for sealed curing storage, wherein the temperature for sealed curing storage is room temperature; by aging, fructo-oligosaccharide in the microorganism extractive solution can be aged to generate aromatic hydrocarbon, which can improve flavor and aromatic hydrocarbon effect.
As a preferred embodiment, in order to increase the active ingredient content of the microbial extract, the availability for human body and reduce the content of harmful substances, the primary fermented microbe in step 2 comprises the following strains in parts by weight according to the sum of the weight of each component: 1 part of lactobacillus casei, 1 part of lactobacillus rhamnosus, 1 part of lactobacillus acidophilus, 1 part of bifidobacterium adolescentis, 1 part of bifidobacterium longum, 0.1 part of lactobacillus reuteri, 0.5 part of lactobacillus crispatus, 0.5 part of lactobacillus paracasei subspecies paracasei, 0.5 part of lactobacillus helveticus, 0.5 part of lactobacillus fermentum, 0.5 part of streptococcus thermophilus, 0.5 part of lactobacillus gasseri, 0.5 part of lactobacillus delbrueckii subspecies bulgaricus, 0.4 part of lactobacillus plantarum, 0.1 part of bifidobacterium lactis, 0.1 part of bifidobacterium bifidum, 0.1 part of bifidobacterium infantis, 0.1 part of bifidobacterium breve, 0.1 part of lactobacillus johnsonii, 0.1 part of lactobacillus salivarius, 0.1 part of lactobacillus subspecies lactis, 0.1 part of lactococcus lactis subspecies cremorium, 0.1 part of lactococcus lactis diacetyl subspecies lactis, and 0.1 part of leuconostoc mesenteroides.
In order to further reduce the content of harmful substances in the microecological extract of the present invention, the microorganisms in step 2 further include microorganisms for degrading organophosphorus pesticides; as the microorganism for degrading the organophosphorus pesticide, bacteria, fungi and algae can be selected, and correspondingly such as:
chlorpyrifos is degraded, and the following bacteria can be selected: bacillus subtilis inaquosum strain n KCTC13429, bacillus cereus ATCC 14579Bacillus safensis F0-36b, sph ingomonas sp, pseudomonas nitroreducens PS-2, pseudomonas fluoroscenc, brucella melitensis, bacillus subtilis, bacillus cereus, klebsiella sp, serratia sp, pseudomonas aeruginosa, pseudomonas sp, agrobacterium sp, bacillus C2A1, the following fungi can be selected: asp ergillus terreus, trichoderma harzianum, penicillium brevicompactum, trichoderma sp, optionally algae Synechocystis PUPCCC 64;
the phoxim can be degraded by the following bacteria: plouiomonas sp.X-1, delftia sp.XS P-1, bacillus subtilis Bs-15, stenotrophoromonas sp.G1, ochrobactum s p.DDV-1; the following fungi may be selected: paecilomyces lailicinus NH-PL-03, trichoode rma atroviride;
degrading trichlorfon by using bacteria Rhodobacter sphaeroides and degrading triazophos by using bacteria Stenotrophomonas sp.G1 and Bacillus sp.TAP-1;
in a preferred embodiment, the microecological extract can be further added with 0.66g of fermentation stock solution, wherein the fermentation stock solution is the microecological extract obtained in the step 2 or the microecological extract obtained in the step 3.
Specifically, in the microecological extraction process of the present invention, the above-listed microorganisms may be selected based on the need, one or a combination of two or more of them may be selected, or other microorganisms not listed may be selected based on the need to be combined with the above-listed microorganisms.
Example 1
A microecological extract for preventing and treating thyroid nodules is prepared from the following components in parts by weight: 15g of ternate buttercup root, 25g of selfheal, 15g of curcuma zedoary, 15g of thunberg fritillary bulb, 15g of radix bupleuri, 15g of radix curcumae, 15g of green tangerine peel, 40g of raw oyster, 15g of pinellia ternate, 30g of astragalus membranaceus, 15g of dried orange peel, 10g of fructus aurantii, 12g of poria cocos, 10g of rhizoma cyperi, 10g of platycodon grandiflorum, 10g of liquorice, 10g of elecampane, 10g of allium macrostemon, 10g of radix ophiopogonis, 10g of cowherb seed, 10g of garden burnet, 10g of radix glehniae, 10g of king tea, 10g of perilla seed, 10g of uncaria, 10g of polygala tenuifolia, 0.1g of omega-3, 0.001g of B-carotene, 0.05g of quercetin and 0.00005g of organic selenium;
a preparation method of a microecological extract for preventing and treating thyroid nodules comprises the following steps:
step 1) taking the components according to a formula, mixing, sterilizing and crushing to obtain a raw material mixture, and adding pepsin into the raw material mixture for enzymolysis to obtain an enzymolysis raw material mixture; the amount of pepsin is 0.5-3 ‰ of the weight of the raw material mixture.
Step 2) carrying out microbial ecological extraction treatment on the raw material mixture obtained in the step 1 by using microorganisms, wherein the extraction treatment specifically comprises carrying out primary fermentation on the raw material mixture subjected to enzymolysis under an acidic condition, wherein the primary fermentation temperature is 35-38 ℃, the primary fermentation time is 15-25 days, and the pH value of the primary fermentation is 2-7; performing static fermentation and chelation on the raw material mixture subjected to acid fermentation at normal temperature, wherein the static fermentation and chelation time is 10-25 days, and the pH value is 2-5; then, filtering, removing filter residues, and collecting the micro-ecological extracting solution;
and 3) sterilizing the microecological extract, and then curing to obtain the microecological extract.
The microorganism for one-time fermentation in the step 2 comprises the following strains in percentage by weight of the sum of the weight of the components: 1 part of lactobacillus casei, 1 part of lactobacillus rhamnosus, 1 part of lactobacillus acidophilus, 1 part of bifidobacterium adolescentis, 1 part of bifidobacterium longum, 0.1 part of lactobacillus reuteri, 0.5 part of lactobacillus crispatus, 0.5 part of lactobacillus paracasei subspecies paracasei, 0.5 part of lactobacillus helveticus, 0.5 part of lactobacillus fermentum, 0.5 part of streptococcus thermophilus, 0.5 part of lactobacillus gasseri, 0.5 part of lactobacillus delbrueckii subspecies bulgaricus, 0.4 part of lactobacillus plantarum, 0.1 part of bifidobacterium lactis, 0.1 part of bifidobacterium bifidum, 0.1 part of bifidobacterium infantis, 0.1 part of bifidobacterium breve, 0.1 part of lactobacillus johnsonii, 0.1 part of lactobacillus salivarius, 0.1 part of lactobacillus subspecies lactis, 0.1 part of lactococcus lactis subspecies cremorium, 0.1 part of lactococcus lactis diacetyl subspecies lactis, and 0.1 part of leuconostoc mesenteroides.
When the microecological extract is orally administered, it is diluted 5-10 times with water.
Example 2
A microecological extract for preventing and treating thyroid nodules is prepared from the following components in parts by weight: 10g of ternate buttercup root, 20g of selfheal, 10g of curcuma zedoary, 10g of thunberg fritillary bulb, 10g of radix bupleuri, 10g of radix curcumae, 10g of green tangerine peel, 30g of raw oyster, 10g of pinellia ternate, 20g of astragalus membranaceus, 10g of dried orange peel, 5g of fructus aurantii, 10g of poria cocos, 8g of rhizoma cyperi, 8g of platycodon grandiflorum, 8g of liquorice, 8g of elecampane, 8g of allium macrostemon, 8g of radix ophiopogonis, 8g of cowherb seed, 8g of garden burnet, 8g of radix glehniae, 8g of king tea, 8g of perilla seed, 8g of uncaria, 8g of polygala tenuifolia, 8g of omega-3.1g of B-carotene, 0.001g of quercetin and 0.00005g of organic selenium;
the preparation method is the same as that of example 1.
When the microecological extract is taken orally, it needs to be diluted 5-10 times with water.
Example 3
A microecological extract for preventing and treating thyroid nodules is prepared from the following components in parts by weight: 20g of ternate buttercup root, 30g of selfheal, 20g of curcuma zedoary, 20g of thunberg fritillary bulb, 20g of radix bupleuri, 20g of radix curcumae, 20g of green tangerine peel, 50g of raw oyster, 20g of pinellia ternate, 40g of astragalus membranaceus, 20g of dried orange peel, 15g of fructus aurantii, 15g of poria cocos, 12g of rhizoma cyperi, 12g of platycodon grandiflorum, 12g of liquorice, 12g of elecampane, 12g of allium macrostemon, 12g of radix ophiopogonis, 12g of cowherb seed, 12g of garden burnet, 12g of radix glehniae, 12g of king tea, 12g of perilla seed, 12g of uncaria, 12g of polygala tenuifolia, 0.1g of omega-3, 0.001g of B-carotene, 0.05g of quercetin and 0.00005g of organic selenium;
the preparation method is the same as that of example 1.
When the microecological extract is taken orally, it needs to be diluted 5-10 times with water.
Table 1: example 1 table of data for active ingredient content tests
Figure BDA0003787222720000131
Experimental example 1
The microecological extract for treating thyroid nodules of example 1 was taken, and the contents of crude polysaccharides, total flavonoids, polyphenols, total saponins, superoxide dismutase and harmful substances, heavy metal lead, were measured and compared with the control group.
Comparative example 1
Taking the components according to the microecological components and the proportion thereof in the embodiment 1, mixing, sterilizing, and then carrying out superfine grinding to obtain a ground raw material mixture; and adding water, decocting for 3 times, decocting for 30min each time, collecting the filtrate obtained by 3 times of decoction through filtration, and combining the filtrates to obtain the filtrate of comparative example 1 (namely the extract of comparative example 1).
The microecological formulation according to example 1 was taken in an amount of 2g each, and the extracts of example 1 and comparative example 1 were obtained by the methods of example 1 and comparative example 1, respectively, 2g of the extracts were concentrated to the same weight (the extract of comparative example 1 was concentrated to the same weight based on the microecological extract of example 1), and the extracts were then tested for the content of active ingredient, respectively, with the test results data shown in the following table:
table 2: table of data for testing the contents of active ingredients in example 1 and comparative example 1
Figure BDA0003787222720000141
As can be seen from the data in table 2, compared with the conventional decoction process of comparative example 1, the microecological extraction of the present invention can release active ingredients better, so that the microecological extract of the present invention has higher nutritional active ingredients, and has better effect of treating thyroid nodules.
The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention should not be limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.

Claims (10)

1. A microecological extract for preventing and treating thyroid nodules is characterized by being prepared from the following components: radix ranunculi ternati, selfheal, curcuma zedoary, thunberg fritillary bulb, radix bupleuri, radix curcumae, pericarpium citri reticulatae viride, raw oyster, pinellia ternate, astragalus mongholicus, pericarpium citri reticulatae, fructus aurantii, poria cocos, rhizoma cyperi, platycodon grandiflorum, liquorice, elecampane, allium macrostemon, radix ophiopogonis, cowherb seed, garden burnet, radix glehniae, royal tea, perilla seed, uncaria, polygala tenuifolia, OMEGA-3, B-carotene, quercetin and organic selenium.
2. The microecological extract for the treatment of thyroid nodules according to claim 1, which is prepared from the following components by weight: 10-20 parts of radix ranunculi ternati, 20-30 parts of selfheal, 10-20 parts of curcuma zedoary, 10-20 parts of thunberg fritillary bulb, 10-20 parts of radix bupleuri, 10-20 parts of radix curcumae, 10-20 parts of pericarpium citri reticulatae viride, 30-50 parts of raw oyster, 10-20 parts of pinellia ternate, 20-40 parts of radix astragali, 10-20 parts of pericarpium citri reticulatae, 5-15 parts of fructus aurantii, 10-15 parts of poria cocos, 8-12 parts of rhizoma cyperi, 8-12 parts of platycodon grandiflorum, 8-12 parts of liquorice, 8-12 parts of elecampane, 8-12 parts of allium macrostemon, 8-12 parts of radix ophiopogonis, 8-12 parts of cowherb seed, 8-12 parts of garden burnet, 8-12 parts of radix glehniae king, 8-12 parts of herb tea, 8-12 parts of perilla seed, 8-12 parts of uncaria, 8-12 parts of polygala tenuifolia, 0.1 part of OMEGA-3, 0.001 part of B-carotene, 0.05 part of quercetin and 0.00005 part of organic selenium.
3. The microecological extract for treating thyroid nodules according to claim 1, wherein the microecological extract comprises 15 parts of ternate buttercup root, 25 parts of prunella vulgaris, 15 parts of curcuma zedoary, 15 parts of thunberg fritillary bulb, 15 parts of radix bupleuri, 15 parts of curcuma aromatica, 15 parts of green tangerine peel, 40 parts of raw oyster shell, 15 parts of pinellia ternate, 30 parts of astragalus membranaceus, 15 parts of dried orange peel, 10 parts of fructus aurantii, 12 parts of poria cocos, 10 parts of rhizoma cyperi, 10 parts of platycodon grandiflorum, 10 parts of liquorice, 10 parts of elecampane, 10 parts of allium macrostemon, 10 parts of radix ophiopogonis, 10 parts of cowherb seed, 10 parts of garden burnet, 10 parts of radix glehniae, 10 parts of king tea, 10 parts of perilla seed, 10 parts of uncaria, 10 parts of polygala tenuifolia, 0.1 part of OMEGA-3, 0.001 part of B-carotene, 0.05 part of quercetin, and 0.00005 part of organic selenium.
4. A preparation method of a microecological extract for preventing and treating thyroid nodules comprises the following steps:
step 1) taking the components according to a formula, mixing, sterilizing and crushing to obtain a raw material mixture, and adding pepsin into the raw material mixture for enzymolysis treatment to obtain an enzymolysis raw material mixture;
step 2) carrying out microbial ecological extraction treatment on the raw material mixture obtained in the step 1 by using microorganisms, wherein the extraction treatment specifically comprises the step of carrying out primary fermentation on the raw material mixture subjected to enzymolysis under an acidic condition, wherein the primary fermentation temperature is 35-38 ℃, the primary fermentation time is 15-25 days, and the primary fermentation pH value is 2-7; performing static fermentation and chelation on the raw material mixture subjected to acid fermentation at normal temperature, wherein the static fermentation and chelation time is 10-25 days, and the pH value is 2-5; then, filtering, removing filter residues, and collecting the micro-ecological extracting solution;
and 3) sterilizing the microecological extract, and then curing to obtain the microecological extract.
5. The method of claim 4, further comprising the step of 4: adding food acceptable auxiliary materials into the microecological extract obtained in the step 3.
6. The method of preparing a microecological extract for the treatment of thyroid nodules according to claim 4, further comprising, after step 4, step 5: heating the microecological extract treated in the step 4 until the auxiliary materials are dissolved, and then sterilizing; the adjuvant is soluble in water.
7. The method of claim 4, wherein the amount of pepsin in step 1 is 0.5 to 3% by weight of the raw material mixture.
8. The method for preparing a microecological extract for the treatment of thyroid nodules according to claim 4, wherein the maturation process in step 3 comprises: and (3) placing the microecological extracting solution into a sterilized curing barrel for sealed curing storage, wherein the temperature for sealed curing storage is room temperature.
9. The method for preparing the microecological extract for the treatment of thyroid nodules according to claim 4, wherein the primary fermentation microorganism in step 2 comprises the following species in parts by weight based on the sum of the weight of each component: 1 part of lactobacillus casei, 1 part of lactobacillus rhamnosus, 1 part of lactobacillus acidophilus, 1 part of bifidobacterium adolescentis, 1 part of bifidobacterium longum, 0.1 part of lactobacillus reuteri, 0.5 part of lactobacillus crispatus, 0.5 part of lactobacillus paracasei subspecies paracasei, 0.5 part of lactobacillus helveticus, 0.5 part of lactobacillus fermentum, 0.5 part of streptococcus thermophilus, 0.5 part of lactobacillus gasseri, 0.5 part of lactobacillus delbrueckii subspecies bulgaricus, 0.4 part of lactobacillus plantarum, 0.1 part of bifidobacterium lactis, 0.1 part of bifidobacterium bifidum, 0.1 part of bifidobacterium infantis, 0.1 part of bifidobacterium breve, 0.1 part of lactobacillus johnsonii, 0.1 part of lactobacillus salivarius, 0.1 part of lactobacillus lactis subspecies lactococcus lactis, 0.1 part of lactococcus lactis subspecies lactis, 0.1 part of lactococcus lactis diacetyl subspecies lactis, and 0.1 part of leuconostoc mesenteroides.
10. The method of claim 4, wherein the microorganisms of step 2 further comprise microorganisms for degrading organophosphorus pesticides.
CN202210945538.4A 2022-08-08 2022-08-08 Microecological extract for preventing and treating thyroid nodules and preparation method thereof Withdrawn CN115300596A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116327885A (en) * 2023-05-29 2023-06-27 吉林华康药业股份有限公司 Pharmaceutical composition for preventing and treating thyroid nodule

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116327885A (en) * 2023-05-29 2023-06-27 吉林华康药业股份有限公司 Pharmaceutical composition for preventing and treating thyroid nodule
CN116327885B (en) * 2023-05-29 2023-07-25 吉林华康药业股份有限公司 Pharmaceutical composition for preventing and treating thyroid nodule

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