CN115282192A - Composition with anti-inflammatory and analgesic effects, experiment and preparation method - Google Patents
Composition with anti-inflammatory and analgesic effects, experiment and preparation method Download PDFInfo
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- 230000003110 anti-inflammatory effect Effects 0.000 title claims abstract description 45
- 239000000203 mixture Substances 0.000 title claims abstract description 36
- 230000001760 anti-analgesic effect Effects 0.000 title claims abstract description 20
- 238000002474 experimental method Methods 0.000 title claims abstract description 13
- 238000002360 preparation method Methods 0.000 title abstract description 16
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 66
- 239000000341 volatile oil Substances 0.000 claims description 52
- 241000699670 Mus sp. Species 0.000 claims description 44
- 238000012360 testing method Methods 0.000 claims description 37
- 241000699666 Mus <mouse, genus> Species 0.000 claims description 30
- 239000013641 positive control Substances 0.000 claims description 30
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 claims description 28
- 206010014025 Ear swelling Diseases 0.000 claims description 27
- 230000000202 analgesic effect Effects 0.000 claims description 27
- 230000005764 inhibitory process Effects 0.000 claims description 25
- 230000000694 effects Effects 0.000 claims description 16
- 210000005069 ears Anatomy 0.000 claims description 15
- 239000003814 drug Substances 0.000 claims description 13
- 240000001307 Myosotis scorpioides Species 0.000 claims description 12
- 239000008096 xylene Substances 0.000 claims description 12
- 230000008961 swelling Effects 0.000 claims description 10
- 241000125175 Angelica Species 0.000 claims description 9
- 235000001287 Guettarda speciosa Nutrition 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 9
- 230000002579 anti-swelling effect Effects 0.000 claims description 8
- 229940079593 drug Drugs 0.000 claims description 8
- 241000213006 Angelica dahurica Species 0.000 claims description 7
- 206010061218 Inflammation Diseases 0.000 claims description 7
- 230000036592 analgesia Effects 0.000 claims description 7
- CGIGDMFJXJATDK-UHFFFAOYSA-N indomethacin Chemical compound CC1=C(CC(O)=O)C2=CC(OC)=CC=C2N1C(=O)C1=CC=C(Cl)C=C1 CGIGDMFJXJATDK-UHFFFAOYSA-N 0.000 claims description 7
- 230000004054 inflammatory process Effects 0.000 claims description 7
- 238000004080 punching Methods 0.000 claims description 7
- 230000002401 inhibitory effect Effects 0.000 claims description 6
- BSYNRYMUTXBXSQ-UHFFFAOYSA-N Aspirin Chemical compound CC(=O)OC1=CC=CC=C1C(O)=O BSYNRYMUTXBXSQ-UHFFFAOYSA-N 0.000 claims description 5
- 229960001138 acetylsalicylic acid Drugs 0.000 claims description 5
- 239000003153 chemical reaction reagent Substances 0.000 claims description 5
- 239000007924 injection Substances 0.000 claims description 5
- 238000002347 injection Methods 0.000 claims description 5
- 239000002199 base oil Substances 0.000 claims description 4
- 229960000905 indomethacin Drugs 0.000 claims description 4
- 210000000683 abdominal cavity Anatomy 0.000 claims description 3
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 238000005520 cutting process Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 230000036407 pain Effects 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 230000003467 diminishing effect Effects 0.000 claims description 2
- 229920002310 Welan gum Polymers 0.000 claims 2
- 241000893536 Epimedium Species 0.000 claims 1
- 235000018905 epimedium Nutrition 0.000 claims 1
- 241000218158 Clematis Species 0.000 description 4
- 238000012449 Kunming mouse Methods 0.000 description 3
- URLKBWYHVLBVBO-UHFFFAOYSA-N Para-Xylene Chemical group CC1=CC=C(C)C=C1 URLKBWYHVLBVBO-UHFFFAOYSA-N 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000035622 drinking Effects 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- KKADPXVIOXHVKN-UHFFFAOYSA-N 4-hydroxyphenylpyruvic acid Chemical compound OC(=O)C(=O)CC1=CC=C(O)C=C1 KKADPXVIOXHVKN-UHFFFAOYSA-N 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 241001428407 Ceramium Species 0.000 description 1
- 241000903946 Clematidis Species 0.000 description 1
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- 238000001514 detection method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 210000003141 lower extremity Anatomy 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 230000008855 peristalsis Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
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- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
- A61K36/716—Clematis (leather flower)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/0004—Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions
- A61K49/0008—Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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Abstract
The invention discloses a composition with anti-inflammatory and analgesic effects, and an experiment and preparation method thereof.
Description
Technical Field
The invention relates to the technical field of anti-inflammatory analgesic drugs and experiments, in particular to a composition with anti-inflammatory analgesic effects, an experiment and a preparation method.
Background
Aiming at the existing essential oil products, health care is mainly performed, an essential oil composition with anti-inflammatory and analgesic effects is not provided, and an experimental preparation method applicable to the preparation and research process is not provided, but the essential oil products are very convenient in the using process. Therefore, a composition with the effects of diminishing inflammation and easing pain, an experiment and a preparation method are provided.
Disclosure of Invention
The invention aims to solve the defects in the prior art, and provides a composition with anti-inflammatory and analgesic effects, an experiment and a preparation method.
In order to achieve the purpose, the invention adopts the following technical scheme:
a composition with anti-inflammatory and analgesic effects is in the form of liquid essential oil, and comprises radix Angelicae Dahuricae essential oil and Ceramium wilsonii essential oil.
An experimental method of a composition with anti-inflammatory and analgesic effects comprises a dimethylbenzene induced mouse ear swelling anti-inflammatory test and a mouse acetic acid writhing analgesic test.
The reagent type used in the xylene-induced mouse ear swelling anti-inflammatory test comprises angelica essential oil.
The reagent used in the acetic acid writhing analgesic test of the mouse comprises the Wixianling essential oil.
Further, the anti-inflammatory test for the mouse ear swelling caused by xylene comprises the following specific steps:
a1, dividing mice into a model group, a positive control group and a single group (1) to (9) according to a randomization principle, wherein each group comprises 10 mice, the positive control group and the single group (1) to (9) are transdermally administered for 2 times at an interval of 2h, the administration type of the positive control group is 1mg/ml indomethacin, the administration types of the group (1) to (9) are essential oil or other medicines with anti-inflammatory and swelling inhibition effects, and the group (4) is angelica essential oil.
And A2, after the last administration for 1 hour, taking the left ear of the mouse as a self control, uniformly coating 40 mu L of dimethylbenzene on the front side and the back side of the right ear of the mouse to cause inflammation, wherein each side is about 20 mu L, killing the mouse by dislocation of cervical vertebra after 30min, cutting off two ears of the mouse, punching the middle of the auricle of two ears by using an automatic ear puncher with the diameter of 8mm, punching the ear at the same position, punching the ear, weighing, and calculating the swelling degree of the ears and the inhibition rate of the medicament.
Ear swelling degree of mouse:
inhibition rate of mouse ear swelling:
and A3, judging whether the essential oil component of the angelica dahurica contained in the composition has stronger anti-inflammatory and swelling inhibition effects or not according to the inhibition rate data.
Further, the concrete steps of the mouse acetic acid writhing analgesia test are as follows:
b1, dividing mice into a model group, a positive control group and a single group (1) to (9) according to a randomization principle, wherein each group comprises 10 mice, the positive control group and the single group (1) to (9) are transdermally administered for 2 times at intervals of 2h, the administration type of the positive control group is aspirin with the concentration of 10mg/ml, the administration types of the group (1) to (9) are all essential oil or other medicines with an analgesic effect, and the group (7) is the Wenxianling essential oil.
And B2, after 30min of the last administration, except the positive control group, injecting 0.6% acetic acid solution into the abdominal cavity of each group of mice to obtain 0.1mL/10g, recording the times of writhing within 20min after the acetic acid injection, the number of mice generating writhing, the time (namely latency time) of first writhing generation, and calculating the percentage of analgesia.
And B3, judging whether the clematis essential oil component contained in the composition has a strong analgesic and inhibitory effect according to the analgesic percentage data.
A preparation method of a composition with anti-inflammatory and analgesic effects comprises mixing the essential oil of radix Angelicae Dahuricae and radix Clematidis, and adding base oil into the mixture to obtain the composition with anti-inflammatory and analgesic effects.
The invention provides a composition with anti-inflammatory and analgesic effects, an experiment and a preparation method, and the composition has the following beneficial effects: the composition in the scheme has the anti-inflammatory effect and the strong analgesic effect, avoids the phenomenon that a plurality of medicines are smeared in the traditional anti-inflammatory and analgesic treatment process, and is favorable for popularization and use.
Drawings
FIG. 1 is a bar graph comparing the effect of inhibition of ear swelling in mice by paraxylene according to the present invention;
FIG. 2 is a bar graph comparing the effect of various groups of the present invention on the writhing inhibition rate of acetic acid-induced mice.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, are used in the orientations and positional relationships indicated in the drawings, which are based on the orientations and positional relationships indicated in the drawings, and are used for convenience of description and simplicity of description, but do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
Example 1
A composition with anti-inflammatory and analgesic effects is in a liquid essential oil state, and comprises radix angelicae essential oil and clematis root essential oil.
Example 2
An experimental method of a composition with anti-inflammatory and analgesic effects comprises xylene-induced ear swelling anti-inflammatory test and acetic acid writhing analgesic test; the reagent type used in the xylene-induced mouse ear swelling anti-inflammatory test comprises angelica essential oil, and the xylene-induced mouse ear swelling anti-inflammatory test comprises the following specific steps:
a1, dividing mice into a model group, a positive control group and a single group from (1) to (9) according to a randomization principle, wherein each group comprises 10 mice, the positive control group and the single group from (1) to (9) are transdermally administered for 2 times at an interval of 2h, the administration type of the positive control group is 1mg/ml indomethacin, the administration types of the group from (1) to (9) are all essential oil or other medicines with anti-inflammatory and swelling inhibition effects, and the group (4) is angelica essential oil.
And A2, after the last administration for 1 hour, taking the left ear of the mouse as a self control, uniformly coating 40 mu L of dimethylbenzene on the front side and the back side of the right ear of the mouse to cause inflammation, wherein each side is about 20 mu L, killing the mouse by dislocation of cervical vertebra after 30min, cutting off two ears of the mouse, punching the middle of the auricle of two ears by using an automatic ear puncher with the diameter of 8mm, punching the ear at the same position, punching the ear, weighing, and calculating the swelling degree of the ears and the inhibition rate of the medicament.
Ear swelling degree of mouse:
inhibition of ear swelling in mice:
and A3, judging whether the essential oil component of the angelica dahurica contained in the composition has stronger anti-inflammatory and swelling inhibition effects or not according to the inhibition rate data.
Test example 1
Based on the above example 2, referring to fig. 1, the anti-inflammatory test for ear swelling caused by xylene comprises the following specific steps:
preparation of test materials
9 essential oils or other medicines with antiinflammatory and swelling inhibiting effects, including radix Angelicae Dahuricae essential oil; xylene, kunming mouse, SPF grade, weight 18-22 g, male. One thousandth electronic balance, a micropipettor, an ultralow temperature refrigerator, a plurality of 1ml syringes, 2ml syringes and 5ml syringes, and an automatic lug puncher.
Dosage design
The anti-inflammatory test is carried out by using mice, the preparation is applied by percutaneous smearing, the maximum dosage is carried out according to the administration area of 3cm multiplied by 3cm after the hair of the mice is removed, and the administration volume is about 0.5ml. In this test, the formulations involved were all administered transdermally at the same dose. The positive control is indometacin 1mg/ml.
Grouping and testing
The test adopts a self-control method, mice are divided into a model control group, a positive control group and single groups (1) to (9) according to a randomization principle, 10 mice in each group are administrated transdermally for 2 times at intervals of 2h, and (4) groups are angelica essential oil.
Establishing a dimethylbenzene-induced mouse ear swelling inflammation model: mice were fasted for 12h before the test, with unlimited drinking. After the last administration for 1h, the left ear of the mouse is taken as a self control, the front and back sides of the right ear of the mouse are uniformly coated with 40 mu L of dimethylbenzene for causing inflammation, each side is about 20 mu L, the mouse is killed by dislocation of cervical vertebra after 30min, the ears of the mouse are cut off, the same position in the middle of auricles of two ears is punched by an automatic ear puncher with the diameter of 8mm, the ears are punched, weighed, and the swelling degree of the ears and the inhibition rate of the medicine are calculated.
Ear swelling degree of mouse:
inhibition of ear swelling in mice:
test results
The results of the different groups of the influence on the mouse ear swelling rate are shown in table 1 and figure 1, after 2 times of continuous administration, after the mouse right ear is subjected to dimethylbenzene to cause ear swelling for 30min, the ears of the right ear of each group of mice are obviously red and swollen, the left ear and the right ear are cut and punched to obtain the ear pieces with the diameters of 8mm, and the weight difference between the right ear and the left ear, namely the swelling degree, is calculated. After administration, each group had a certain inhibitory effect. Wherein the positive control group significantly inhibited ear swelling (p < 0.05); (4) the group had a strong effect of inhibiting ear swelling.
TABLE 1 Effect of xylene on ear swelling in mice
Conclusion
As can be seen from the swelling inhibition rate of mouse ear swelling, compared with the positive control group, the (1), (2), (4) and (8) have stronger swelling inhibition effects, wherein the swelling inhibition rate of the (4) is the highest, so the angelica essential oil of the group (4) is selected as one of the preparation components of the composition.
Example 3
An experimental method of a composition with anti-inflammatory and analgesic effects comprises a dimethylbenzene induced mouse ear swelling anti-inflammatory test and a mouse acetic acid writhing analgesic test, wherein the mouse acetic acid writhing analgesic test specifically comprises the following steps:
b1, dividing mice into a model group, a positive control group and a single group (1) to (9) according to a randomization principle, wherein each group comprises 10 mice, the positive control group and the single group (1) to (9) are transdermally administered for 2 times at intervals of 2h, the administration type of the positive control group is aspirin with the concentration of 10mg/ml, the administration types of the group (1) to (9) are all essential oil or other medicines with an analgesic effect, and the group (7) is the Wenxianling essential oil.
And B2, after 30min of the last administration, except the positive control group, injecting 0.6% acetic acid solution 0.1mL/10g into the abdominal cavity of the mice of other groups, recording the times of writhing within 20min after acetic acid injection, counting the number of the mice with writhing, the time (namely the latency time) for the first writhing, and calculating the analgesic percentage.
And B3, judging whether the Weixianling essential oil component contained in the composition has a strong analgesic and inhibitory effect or not according to the analgesic percentage data.
Test example 2
Based on the above example 3, referring to fig. 2, the anti-inflammatory test for ear swelling caused by xylene comprises the following specific steps:
preparation of test materials
9 kinds of essential oil or other medicine with analgesic effect, including Weixianling essential oil; acetic acid solution (prepared into 0.6% acetic acid solution before use), aspirin (prepared into solution before use), kunming mouse (SPF grade, weight 18-22 g, half each), one thousandth electronic balance, micropipettor, and a plurality of injectors of 1ml, 2ml and 5ml
Dosage design
The test adopts mice to carry out analgesia test, the preparation adopts percutaneous smearing administration, the maximum dosage is administered according to the administration area of 3cm multiplied by 3cm after the hair of the mice is removed, and the administration volume is about 0.5ml. In this experiment, the formulations involved were all administered transdermally at the same dose. The positive control is aspirin 10mg/ml.
Grouping and testing
Animal grouping: dividing mice into a model control group, a positive control group and single groups (1) to (9) according to a randomization principle, wherein each group contains 10 mice, and transdermal administration is carried out for 2 times at an interval of 2h, and the group (7) contains the Weixianling essential oil.
Establishing an acetic acid induced mouse pain model: mice were fasted for 12h before the test, with unlimited drinking. Healthy male Kunming mice are subjected to intraperitoneal injection of 0.6% acetic acid solution 0.1mL/10g after 30min of the last administration except for a control group, the times of writhing within 20min after acetic acid injection are recorded, the number of mice generating writhing is counted, the time (namely latency time) of the first writhing is taken, and the percentage of analgesia is calculated.
Detection indexes are as follows: the number of writhing of mice within 20min after acetic acid injection was recorded.
Percent analgesia was calculated based on the recorded writhing number.
Test results
The influence of each component on the writhing response of the mice caused by acetic acid is shown in the table 2 of figure 2, the writhing response of the mice (abdomen contraction and concave, hind limb involuntary extension, hip elevation and peristalsis) occurs 2min after the model group, the writhing occurrence time of the model group is short, the writhing frequency is high, and the model group shows that the model formation is successful. Compared with a model group, the mice have different writhing reaction conditions after the administration of the positive control group and different administration groups, wherein the latency time of the positive control group and the (7) group for writhing reaction is obviously prolonged, the writhing reaction is generated after 15min, the writhing reaction of the other groups is quicker, the writhing times are more, and the mice also have certain analgesic effect compared with the model group. Overall, each component had a certain analgesic effect, but group (7) was the best.
TABLE 2 Effect of analgesia by the acetate writhing method in mice
Conclusion
As can be seen from the writhing inhibition rate of the mice, the positive control group has the best analgesic effect, but each single component also has a certain analgesic inhibition effect, and the analgesic inhibition rate effect is the best (7), so the weixianling essential oil in the group (7) is selected as one of the preparation components of the composition.
Example 4:
with reference to the above examples and experimental examples, a method for preparing a composition having anti-inflammatory and analgesic effects, aiming at the analgesic effect of the clematis root essential oil component and the anti-inflammatory and swelling effect of the dahurian angelica root essential oil component, the clematis root essential oil component and the dahurian angelica root essential oil component are mixed, and the base oil is added to the mixed solution to obtain the composition having anti-inflammatory and analgesic effects.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (5)
1. The composition with the effects of diminishing inflammation and easing pain is characterized by being in a liquid essential oil state and comprising angelica essential oil and welan essential oil.
2. The method of claim 1, comprising an anti-inflammatory test for swelling of ear in mice by xylene and an analgesic test for writhing in acetic acid in mice;
the reagent type used in the xylene-induced mouse ear swelling anti-inflammatory test comprises angelica essential oil;
the reagent type used in the mouse acetic acid writhing pain-relieving test comprises the welan epimedium essential oil.
3. The method for testing the composition with anti-inflammatory and analgesic effects as claimed in claim 2, wherein the xylene-induced ear swelling anti-inflammatory test of the mouse comprises the following steps:
a1, dividing mice into a model group, a positive control group and a single group (1) to (9) according to a randomization principle, wherein each group comprises 10 mice, the positive control group and the single group (1) to (9) are transdermally administered for 2 times at an interval of 2h, the administration type of the positive control group is 1mg/ml indomethacin, the administration types of the group (1) to (9) are essential oil or other medicines with anti-inflammatory and swelling inhibition effects, and the group (4) is angelica essential oil;
a2, after the last administration for 1 hour, taking the left ear of the mouse as a self control, uniformly coating 40 mu L of dimethylbenzene on the front side and the back side of the right ear of the mouse to cause inflammation, wherein each side is about 20 mu L, killing the mouse by dislocation of cervical vertebra after 30min, cutting off two ears of the mouse, using an automatic ear puncher with the diameter of 8mm to punch a hole in the same position in the middle of auricles of two ears, punching the ears, weighing, and calculating the swelling degree of the ears and the inhibition rate of the medicament;
ear swelling degree of mouse:
inhibition rate of mouse ear swelling:
and A3, judging whether the essential oil component of the angelica dahurica contained in the composition has stronger anti-inflammatory and swelling inhibition effects or not according to the inhibition rate data.
4. The experimental method of the composition with anti-inflammatory and analgesic effects as claimed in claim 2, wherein the concrete steps of the mouse acetic writhing analgesic test are as follows:
b1, dividing mice into a model group, a positive control group and a single group from (1) to (9) according to a randomization principle, wherein each group comprises 10 mice, the positive control group and the single group from (1) to (9) are transdermally administered for 2 times at an interval of 2h, the administration type of the positive control group is aspirin with the concentration of 10mg/ml, the administration types of the group from (1) to (9) are all essential oil or other medicines with an analgesic effect, and the group (7) is the Wenxianling essential oil;
b2, after 30min of the last administration, except the positive control group, injecting 0.6% acetic acid solution 0.1mL/10g into the abdominal cavity of the mice of other groups, recording the times of writhing within 20min after the acetic acid injection, counting the number of the mice generating writhing, the time (namely the latency time) of the first writhing, and calculating the percentage of analgesia;
and B3, judging whether the Weixianling essential oil component contained in the composition has a strong analgesic and inhibitory effect or not according to the analgesic percentage data.
5. The method for preparing a composition with anti-inflammatory and analgesic effects as claimed in any one of claims 2 to 4, wherein the composition with anti-inflammatory and analgesic effects is obtained by mixing the essential oil of Weixianling and the essential oil of Angelica dahurica with the base oil, and adding the base oil to the mixture, in order to inhibit the analgesic effect of the essential oil of Weixianling and the anti-inflammatory and swelling effect of the essential oil of Angelica dahurica.
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Citations (4)
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CN104928035A (en) * | 2015-05-26 | 2015-09-23 | 南京泽朗医药科技有限公司 | Extraction method for volatile oil of clematis root |
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CN112957441A (en) * | 2021-03-03 | 2021-06-15 | 董明姣 | A topical medicinal film for dispelling pathogenic wind, removing dampness, warming and dredging channels and collaterals, promoting blood circulation and relieving pain, and its preparation method |
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