CN105816489A - Preparation method of durio zibethinus murr shell extract and application of durio zibethinus murr shell extract - Google Patents
Preparation method of durio zibethinus murr shell extract and application of durio zibethinus murr shell extract Download PDFInfo
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- CN105816489A CN105816489A CN201610242051.4A CN201610242051A CN105816489A CN 105816489 A CN105816489 A CN 105816489A CN 201610242051 A CN201610242051 A CN 201610242051A CN 105816489 A CN105816489 A CN 105816489A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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Abstract
The invention discloses a preparation method of durio zibethinus murr shell extract. The method comprises the following steps: (1), taking white flesh endocarp of durian fruit skin, cleaning, cutting and drying the white flesh endocarp to constant weight; (2), crushing; (3), adding powder obtained in the step (2) into an ethyl alcohol extractant according to certain ratio of material to solution; (4), performing reflux extraction on a thermostat water bath; (5), performing filtration under reduced pressure through a Buchner funnel, combining filtrate, performing concentration through a rotary evaporator, drying, thereby obtaining the durio zibethinus murr shell extract. The invention further discloses application of the durio zibethinus murr shell extract to preparation of anti-inflammatory, cough suppressant and analgesic drugs, and the preparation of the drugs adopts capsules, tablets, granules, sirup, oral preparation and injection.
Description
Technical field
The present invention relates to field of medicaments, be specifically related to the preparation method and applications of a kind of durian shell extract.
Background technology
Durio Zibethinus murr, also known as Fructus nephelii topengii, is the fruit of dietotherapeutic, and rich in abundant mineral and trace unit number, many human bodies have plurality of health care functions.Modern study shows that durian shell has the multiple nutritional labeling useful to human body, rich in the trace element such as calcium, ferrum and several amino acids, has certain medical value.Southeast Asia and China South China resident commonly use durian shell Baoshang, decoct water etc., reach that pathogenic fire reducing solution is stagnant, blood yiqi, moisten the effects such as yin nourishing, but at present for the most rare report of pharmacologically active of durian shell.
Summary of the invention
The preparation method and applications of a kind of durian shell extract are it is an object of the present invention to provide.
The preparation method of a kind of durian shell extract, comprises the following steps:
(1) take the white capsule endothelium in durian pericarp, be chopped into sheet after cleaning up, put in the drying baker of 90-110 DEG C and dry to constant weight；
(2), after skin graft adds pulverizer pulverizing in the white capsule that will dry, cross 100 ~ 300 mesh sieves and be prepared as powder；
(3) powder step (2) prepared adds concentration by certain solid-liquid ratio is in 20%-80% ethanol extraction agent；
(4) reflux, extract, on thermostat water bath, refluxes 2-3 time, every time backflow 30-60 minute；
(5) use buchner funnel filtration under diminished pressure, merging filtrate, concentrate by rotary evaporator, be drying to obtain durian shell extract.
Preferably, the solid-liquid ratio of the extractant added in step (3) can be 1:20,1:40,1:40,1:50, and when wherein solid-liquid ratio is 1:50, extracted amount is optimal.
Preferably, in step (3), the optium concentration of ethanol is 80%.
Preferably, in step (4), optimal reflow's cycle is 3 times, and optimal return time is 30 minutes.
Another object of the present invention is to provide a kind of durian shell extract utilizing described method to prepare.
A further object of the present invention is the application providing described a kind of durian shell extract in preparation antiinflammatory, cough-relieving, analgesic.
The dosage form of described medicine is capsule, tablet, granule, syrup, oral formulations and injection.
A kind of durian shell method for preparing extractive of the present invention is simple, in the aspects such as anti-various active chronic inflammations, cough-relieving, analgesia all well effect, develops the medical value of durian shell.
Detailed description of the invention
Below in conjunction with concrete condition, the detailed description of the invention of the present invention is described in detail.
Embodiment 1
The preparation method of a kind of durian shell extract, comprises the following steps:
(1) take the white capsule endothelium in durian pericarp, be chopped into sheet after cleaning up, put in the drying baker of 100 DEG C and dry to constant weight；
(2), after skin graft adds pulverizer pulverizing in the white capsule that will dry, cross 300 mesh sieves and be prepared as powder；
(3) by step (2) prepare powder by solid-liquid ratio be 1:50 add concentration be in 80% ethanol extraction agent；
(4) reflux, extract, on thermostat water bath, refluxes 3 times, every time backflow 30 minutes；
(5) use buchner funnel filtration under diminished pressure, merging filtrate, concentrate by rotary evaporator, be drying to obtain durian shell extract.
Embodiment 2
A kind of research Oleum Tiglii induced mice ear swelling experiment of durian shell extract antiinflammatory action.
Take mice 50, be randomly divided into 5 groups, often group 10, i.e. Normal group (distilled water 10mg.kg-1), positive drug control group (dexamethasone, 1mg.kg-1), extract low dose group (100mg.kg-1), dosage group (300mg.kg in extract-1), extract high dose group (900mg.kg-1).Each treated animal gastric infusion every day 1 time, successive administration 5 days, last is administered latter 1 hour, by 3g.L-1The appropriate uniform application of Oleum Tiglii acetone soln, at mouse right ear edge middle body, makes mice produce ear and causes inflammation.After causing inflammation, 30min is administered once again, mice breaks after 4h cervical vertebra put to death, cut two ears, lay round auricle at same position respectively with diameter 8mm card punch, weigh mouse right ear weight using its difference as inflammatory swelling degree with analytical balance, and calculate swelling inhibition percentage.Result is as shown in table 1.
Table 1, the experimental result that Oleum Tiglii induced mice ear swelling is affected by extract
| Group | Dosage/mg.kg-1 | The ear method of double differences is different/mg | Suppression ratio/% |
| Normal group | 10 | 23.6±2.40 | 0.00 |
| Positive drug control group | 1 | 10.1±2.32 | 57.20 |
| Extract low dose group | 100 | 20.9±2.30 | 11.44 |
| Dosage group in extract | 300 | 20.0±3.32 | 15.25 |
| Extract high dose group | 900 | 19.3±2.42 | 18.22 |
Compared with blank group, extract middle and high dosage group has certain suppression Oleum Tiglii to cause the effect of mice ear.
Embodiment 3
The research ammonia of a kind of durian shell extract antitussive action causes mice and causes to cough experiment.
Take mice 50, be randomly divided into 5 groups, often group 10, i.e. Normal group (distilled water 10mg.kg-1), positive drug control group (codeine, 20mg.kg-1), extract low dose group (100mg.kg-1), dosage group (300mg.kg in extract-1), extract high dose group (900mg.kg-1).Each treated animal gastric infusion every day 1 time, successive administration 3 days, last is administered latter 1 hour, by mice by being only placed in 5L beaker bell jar, constant-pressure atomization strong aqua ammonia, observes the incubation period (time from starting to be sprayed to cause mice cough for the first time) of mouse cough, and records mouse cough number of times (typical case's cough action: contraction of abdominal muscle or contracting breast in 2min, magnify mouth simultaneously, cough sound).Result is as shown in table 2.
Table 2, extract causes mice and causes to cough the experimental result of reaction impact ammonia
| Group | Dosage/mg.kg-1 | Incubation period/s | Cough number of times/n |
| Normal group | 10 | 55.7±10.6 | 13.4±2.9 |
| Positive drug control group | 20 | 67.1±16.3 | 9.6±3.7 |
| Extract low dose group | 100 | 52.7±13.4 | 9.7±3.5 |
| Dosage group in extract | 300 | 68.9±14.5 | 9.8±3.4 |
| Extract high dose group | 900 | 83.1±22.1 | 7.8±4.0 |
Compared with blank group, extract can extend the incubation period of the mouse cough that ammonia causes to a certain extent.
Embodiment 4
The research acetic acid of a kind of durian shell extract analgesic activity causes mouse writhing reaction experiment.
Take mice 50, be randomly divided into 5 groups, often group 10, i.e. Normal group (distilled water 10mg.kg-1), positive drug control group (aspirin, 200mg.kg-1), extract low dose group (100mg.kg-1), dosage group (300mg.kg in extract-1), extract high dose group (900mg.kg-1).Each treated animal gastric infusion every day 1 time, successive administration 3 days, last is administered latter 1 hour, only inject 0.7% acetum 0.2ml/ to respectively each mouse peritoneal, observe the incubation period (causing the mice writhing time for the first time from starting to be expelled to) of mouse writhing, and in recording 15min mouse writhing number of times (abdominal part indent, stretches hind leg, buttocks is raised), and calculate analgesia percentage rate.Result is as shown in table 2.
Table 2, extract Dichlorodiphenyl Acetate causes the experimental result of mouse writhing reaction impact
| Group | Dosage/mg.kg-1 | Incubation period/s | Writhing number of times/n | Suppression ratio/% |
| Normal group | 10 | 212.3±32.5 | 36.9±10.33 | 0.00 |
| Positive drug control group | 200 | 850.2±123.1 | 2.2±1.88 | 94.01 |
| Extract low dose group | 100 | 220.5±36.6 | 38.0±12.9 | -3.02 |
| Dosage group in extract | 300 | 250.7±45.8 | 30.9±10.8 | 16.81 |
| Extract high dose group | 900 | 363.7±117.0 | 24.1±8.5 | 34.73 |
Compared with blank group, extract can extend acetic acid to a certain extent and cause the incubation period of mouse writhing reaction.
Claims (7)
1. the preparation method of a durian shell extract, it is characterised in that comprise the following steps:
(1) take the white capsule endothelium in durian pericarp, be chopped into sheet after cleaning up, put in the drying baker of 90-110 DEG C and dry to constant weight；
(2), after skin graft adds pulverizer pulverizing in the white capsule that will dry, cross 100 ~ 300 mesh sieves and be prepared as powder；
(3) powder step (2) prepared adds concentration by certain solid-liquid ratio is in 20%-80% ethanol extraction agent；
(4) reflux, extract, on thermostat water bath, refluxes 2-3 time, every time backflow 30-60 minute；
(5) use buchner funnel filtration under diminished pressure, merging filtrate, concentrate by rotary evaporator, be drying to obtain durian shell extract.
The preparation method of a kind of durian shell extract the most according to claim 1, it is characterised in that the solid-liquid ratio of the extractant added in step (3) can be 1:20,1:40,1:40,1:50, and when wherein solid-liquid ratio is 1:50, extracted amount is optimal.
The preparation method of a kind of durian shell extract the most according to claim 1, it is characterised in that in step (3), the optium concentration of ethanol is 80%.
The preparation method of a kind of durian shell extract the most according to claim 1, it is characterised in that in step (4), optimal reflow's cycle is 3 times, and optimal return time is 30 minutes.
5. a kind of durian shell extract prepared according to method any one of claim 1-4.
A kind of durian shell extract (extract) the most according to claim 5 application in preparation antiinflammatory, cough-relieving, analgesic.
Application the most according to claim 6, it is characterised in that the dosage form of described medicine is capsule, tablet, granule, syrup, oral formulations and injection.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610242051.4A CN105816489A (en) | 2016-04-19 | 2016-04-19 | Preparation method of durio zibethinus murr shell extract and application of durio zibethinus murr shell extract |
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|---|---|---|---|
| CN201610242051.4A CN105816489A (en) | 2016-04-19 | 2016-04-19 | Preparation method of durio zibethinus murr shell extract and application of durio zibethinus murr shell extract |
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| CN105816489A true CN105816489A (en) | 2016-08-03 |
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| CN201610242051.4A Withdrawn CN105816489A (en) | 2016-04-19 | 2016-04-19 | Preparation method of durio zibethinus murr shell extract and application of durio zibethinus murr shell extract |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110934271A (en) * | 2019-05-23 | 2020-03-31 | 合肥中科前方生物科技发展有限公司 | Durian peel and pulp crisp chips and making method thereof |
| CN112022885A (en) * | 2020-05-09 | 2020-12-04 | 扬州工业职业技术学院 | Durian shell extracting solution, preparation method thereof and antibacterial mask |
| CN114391650A (en) * | 2022-02-09 | 2022-04-26 | 麦迪尔(厦门)生物科技有限公司 | Durian shell full-utilization method, durian shell powder prepared by using durian shell full-utilization method and application of durian shell powder |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1631412A (en) * | 2004-12-28 | 2005-06-29 | 罗忠 | Medicinal extractive with favorable inflammation eliminating and pain easing function and its formulation |
| CN101244095A (en) * | 2007-06-07 | 2008-08-20 | 东莞市竟恒流通研究所 | New purpose of durio zibethinus L. shell extract |
-
2016
- 2016-04-19 CN CN201610242051.4A patent/CN105816489A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1631412A (en) * | 2004-12-28 | 2005-06-29 | 罗忠 | Medicinal extractive with favorable inflammation eliminating and pain easing function and its formulation |
| CN101244095A (en) * | 2007-06-07 | 2008-08-20 | 东莞市竟恒流通研究所 | New purpose of durio zibethinus L. shell extract |
Non-Patent Citations (1)
| Title |
|---|
| 吴敏芝: "榴莲壳提取的制备及其抗炎镇痛作用的研究", 《医药卫生科技辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110934271A (en) * | 2019-05-23 | 2020-03-31 | 合肥中科前方生物科技发展有限公司 | Durian peel and pulp crisp chips and making method thereof |
| CN112022885A (en) * | 2020-05-09 | 2020-12-04 | 扬州工业职业技术学院 | Durian shell extracting solution, preparation method thereof and antibacterial mask |
| CN114391650A (en) * | 2022-02-09 | 2022-04-26 | 麦迪尔(厦门)生物科技有限公司 | Durian shell full-utilization method, durian shell powder prepared by using durian shell full-utilization method and application of durian shell powder |
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Application publication date: 20160803 |