CN107576767A - A kind of research method of polygonum aubertii Henry analgesic and anti-inflammatory effects - Google Patents
A kind of research method of polygonum aubertii Henry analgesic and anti-inflammatory effects Download PDFInfo
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Abstract
The invention discloses a kind of research method of polygonum aubertii Henry analgesic and anti-inflammatory effects, this method comprises the following steps：Step 1, the influence to thermostimulation induced mice pain；Step 2, the influence to chemical stimulation induced mice pain；Step 3, mice caused by dimethylbenzene xylene ear swelling test；Step 4, egg cause pedal swelling experiment；Step 5, statistical method.The present invention lays a good foundation for the research of polygonum aubertii Henry analgesic and anti-inflammatory effects.
Description
Technical field
The invention belongs to pharmaceutical technology field, is related to a kind of research method of polygonum aubertii Henry analgesic and anti-inflammatory effects.
Background technology
Polygonum aubertii Henry Polygonum aubertii Henry are perennial fruticuli.Stem length reaches several meters, closely upright or winding,
Surface reddish violet, it is hairless, it is solid, it is near wooden.Leafage gives birth to, abundant, and blade is avette or wealthy triangle, long 1~5 centimetre, wide by 0.5~
3 centimetres, top point, the nearly heart-shaped or flechette-type of base portion, leaf margin is wavy, and two sides is hairless；Petiole is thin and delicate, long 0.5~2.5 centimetre；Flower
Sequence is coniform, axillary or basidixed, hairiness on floral axis；Bract film quality, brown, funnel-form, the anxious point in top, the interior flower of tool 2~8 per bud；
Bennet is thin, long 3~4 millimeters, bottom tool joint；The drastic crack of perianth 5, baby pink or white, 3 larger, the backs in tapel outside
Have wing, increase during fruit, base portion is downward；Profile is in obovate during perianth fruit, and stamen 8 is shorter than perianth, and filigree middle and lower part is wider, base
Portion has pubescence；Style is extremely short, column cap head.Achene is avette, has 3 ribs, dark brown is close by little particle, micro- glossy, is wrapped in and harbors
In perianth.7~August of florescence, 8~September of fruiting period.
In the prior art, the relevant report on polygonum aubertii Henry analgesic and anti-inflammatory effects is not found.
The content of the invention
It is an object of the invention to provide a kind of research method of polygonum aubertii Henry analgesic and anti-inflammatory effects.
Its concrete technical scheme is：
A kind of research method of polygonum aubertii Henry analgesic and anti-inflammatory effects, comprises the following steps：
Step 1, the influence to thermostimulation induced mice pain
(1) regulating thermostatic
Intelligent 55.0 ± 0.5 DEG C of hot-plate instrument design temperature, preheat 10min.
(2) qualified mouse is screened
(3) medicine effect is observed
Step 2, the influence to chemical stimulation induced mice pain
Take healthy mice 110, body weight 18~22g, every group 10, random point 11 groups, blank control (physiological saline) group,
Positive control (aspirin) group, the high, medium and low dosage group of polygonum aubertii Henry.According to medicinal material 2~6g of usual amounts, this experiment is orally to give birth to
Dose 6g calculates according to the reduction formula of experimental animal and people's dosage as adult's (60kg) maximum consumption per day, obtains little Bai
The dosage (1.2g/Kg) of mouse.If this is middle dose group, plus-minus sets up high dose and low dosage separately in this data.That is high dose
Group (2.4g/Kg), middle dose group (1.2g/Kg), low dose group (0.6g/Kg).Positive controls are administered by 0.4g/Kg, blank
Control group physiological saline gavage.
1 time a day, after continuous gavage 7d, then 0.2mL0.6% acetum is injected intraperitoneally, observes and records small in 15min
Writhing response (belly indent, the stretching, extension hind leg, buttocks are raised) number of mouse simultaneously calculates each cell mean and suppresses percentage.
Step 3, mice caused by dimethylbenzene xylene ear swelling test
Healthy mice 110,18~22g of body weight is taken, is randomly divided into 11 groups, every group 10, blank control (physiological saline)
Group, positive control (aspirin) group, the high, medium and low dosage group of polygonum aubertii Henry.According to medicinal material 2~6g of usual amounts, this experiment is with oral
Crude drug amount 6g is calculated according to the reduction formula of experimental animal and people's dosage, obtained small as adult's (60kg) maximum consumption per day
The dosage (1.2g/Kg) of white mouse.If this is middle dose group, plus-minus sets up high dose and low dosage separately in this data.I.e. high agent
Amount group (2.4g/Kg), middle dose group (1.2g/Kg), low dose group (0.6g/Kg) blank control group gavage physiological saline；It is positive
Control group is administered by 0.4g/Kg dosage.
1 time/d of gastric infusion, continuous gavage 7d, after every group of mouse continuous gavage is administered 1 week, after last dose 1h, in each
Mouse auris dextra is two-sided to be coated with dimethylbenzene 0.1ml/ only, and left ear is not applied as control, is put to death after 1h, and it is wide to cut left and right helix, uses diameter
Auricle is taken respectively at left and right ear same position for 3mm card punch, is weighed, using the ear weight difference of left and right two as swelling, is calculated swollen
Swollen rate and swelling inhibiting rate.
Step 4, egg cause pedal swelling experiment
Healthy SD rat 72, body weight 180g-220g is taken, is randomly divided into 12 groups, every group 6, blank control (physiology salt
Water) group, model group, positive control (aspirin) group, the high, medium and low dosage group of polygonum aubertii Henry.Foundation medicinal material 2~6g of usual amounts, this
Experiment is using oral crude drug amount 6g as adult's (60kg) maximum consumption per day, according to experimental animal and the reduction formula meter of people's dosage
Calculate, obtain the dosage (0.6g/Kg) of rat).If this is middle dose group, plus-minus sets up high dose and low dose separately in this data
Amount.That is high dose group (1.2g/Kg), middle dose group (0.6g/Kg), low dose group (0.3g/Kg).Blank control group gavage physiology
Salt solution；Positive controls are administered by 2mg/10g dosage.
1 times/day, continuous gavage 7d of gastric infusion, measured before last dose and recorded each with rat's foot volume measuring instrument
The left back sufficient volume of mouse.After last dose 30min, except blank group, egg is subcutaneously injected in rat right hind leg vola pedis respectively in remaining each group
Clear 0.1mL/ only causes inflammation.Then, in 1h, 2h, 3h, 4h, 5h, 6h with being measured with rat's foot volume measuring instrument and record right metapedes
Volume, so that the difference of vola pedis volume is swelling before and after inflammation, and calculates each cell mean and suppress percentage.
Step 5, statistical method
Experimental data withRepresent, analyzed using the statistical softwares of spss 16.0, t is examined between carrying out group.
Further, in step 1, the qualified mouse of screening is specially：
Healthy female mouse is taken, 18~22g of body weight, 1 is placed on hot-plate instrument every time, to place hot-plate instrument to there is mouse
(s) is the pain threshold of the mouse the time required to licking metapedes.Metapedes is licked in 5s or just after 30s or the mouse of jump abandons, select conjunction
Lattice mouse 110, every group 10, it is randomly divided into 11 groups, blank control (physiological saline) group, positive control (aspirin) group,
The high, medium and low dosage group of polygonum aubertii Henry.Normal pain threshold before being administered after packet using 2 pain threshold average values as the mouse, and remember
Record.
Further, in step 1, the observation medicine effect
According to medicinal material 2~6g of usual amounts, this experiment using oral crude drug amount 6g as (60kg) maximum consumption per day of being grown up, according to
Experimental animal and the reduction formula of people's dosage calculate, and obtain the dosage (1.2g/Kg) of small white mouse.If this is middle dose group,
Plus-minus sets up high dose and low dosage separately in this data.That is high dose group (2.4g/Kg), middle dose group (1.2g/Kg), low dosage
Group (0.6g/Kg).Positive controls are administered by 0.4g/Kg, blank control group physiological saline gavage.
By above-mentioned 1 time/d of dosage gastric infusion, continuous gavage 7d, 15 after last dose, 30,45, the 60min measure threshold of pain
Value, if the pain threshold of mouse is calculated more than 60s, its pain threshold with 60s after administration.
Compared with prior art, beneficial effects of the present invention：
The study result show that：
1st, hot plate method result：Significantly increased in the pain threshold of 30min, 45min and 60min polygonum aubertii Henry each group, with sky
White control group more has significant difference, prompts former plant to have obvious analgesic activity to mice pain caused by thermostimulation.
As dosage increases it can be seen from polygonum aubertii Henry senior middle school low dose group, pain threshold is increase tendency.
2nd, writhing method result：The writhing number of aspirin group and polygonum aubertii Henry each group significantly reduces, with blank control group
More there is significant difference, former plant has obvious analgesic activity to pain caused by chemical stimulation.Polygonum aubertii Henry middle dose group
Inhibiting rate reaches highest 30.9%, in each dosage group of polygonum aubertii Henry, middle dose group inhibiting rate highest.
3rd, mice caused by dimethylbenzene xylene ear swelling test result：Aspirin group and polygonum aubertii Henry each group can be to small white mouse auricles
Swelling plays inhibitory action, polygonum aubertii Henry high dose group and middle dose group and significant difference more be present with blank control group, says
The small white mouse auricle edema of bright polygonum aubertii Henry paraxylene induction has obvious antiinflammatory action.And antiinflammatory action and dosage are in positive
Close.
4th, influence result of the polygonum aubertii Henry to rat toes swelling caused by egg white：Aspirin group and Radix Polygoni aubertii extract each group
Inhibitory action can be played to rat toes swelling, polygonum aubertii Henry high dose group is more deposited since 120min with blank control group
In significant difference, the rat toes swelling that display polygonum aubertii Henry is induced egg white has obvious antiinflammatory action.Each senior middle school of polygonum aubertii Henry
Low dose group result, antiinflammatory action and dosage positive correlation.
Embodiment
Technical scheme is described in more detail with reference to specific embodiment.
1 analgesic activity is studied
1.1 material
1.1.2 medicine
Polygonum aubertii Henry stem extraction freeze-dried powder.Aspirin effervescent tablet (AstraZeneca pharmaceutical Co. Ltd, lot number
1401102).Glacial acetic acid (analysis is pure), physiological saline.
1.1.3 instrument
1.2 method
1.2.1 to the influence of thermostimulation induced mice pain
(1) regulating thermostatic
Intelligent hot-plate instrument design temperature (55.0 ± 0.5) DEG C, preheat 10min.
(2) qualified mouse is screened
Healthy female mouse is taken, 18~22g of body weight, 1 is placed on hot-plate instrument every time, to place hot-plate instrument to there is mouse
(s) is the pain threshold of the mouse the time required to licking metapedes.Metapedes is licked in 5s or just after 30s or the mouse of jump abandons, select conjunction
Lattice mouse 110, every group 10, it is randomly divided into 11 groups, blank control (physiological saline) group, positive control (aspirin) group,
The high, medium and low dosage group of polygonum aubertii Henry.Normal pain threshold before being administered after packet using 2 pain threshold average values as the mouse, and remember
Record.
(3) medicine effect is observed
According to medicinal material 2~6g of usual amounts, this experiment using oral crude drug amount 6g as (60kg) maximum consumption per day of being grown up, according to
Experimental animal and the reduction formula of people's dosage calculate, and obtain the dosage (1.2g/Kg) of small white mouse.If this is middle dose group,
Plus-minus sets up high dose and low dosage separately in this data.That is high dose group (2.4g/Kg), middle dose group (1.2g/Kg), low dosage
Group (0.6g/Kg).Positive controls are administered by 0.4g/Kg, blank control group physiological saline gavage.
By above-mentioned 1 time/d of dosage gastric infusion, continuous gavage 7d, 15 after last dose, 30,45, the 60min measure threshold of pain
Value, if the pain threshold of mouse is calculated more than 60s, its pain threshold with 60s after administration.
1.2.2 to the influence of chemical stimulation induced mice pain
Take healthy mice 110, body weight 18~22g, every group 10, random point 11 groups, blank control (physiological saline) group,
Positive control (aspirin) group, the high, medium and low dosage group of polygonum aubertii Henry.According to medicinal material 2~6g of usual amounts, this experiment is orally to give birth to
Dose 6g calculates according to the reduction formula of experimental animal and people's dosage as adult's (60kg) maximum consumption per day, obtains little Bai
The dosage (1.2g/Kg) of mouse.If this is middle dose group, plus-minus sets up high dose and low dosage separately in this data.That is high dose
Group (2.4g/Kg), middle dose group (1.2g/Kg), low dose group (0.6g/Kg).Positive controls are administered by 0.4g/Kg, blank
Control group physiological saline gavage.
1 time a day, after continuous gavage 7d, then 0.2mL0.6% acetum is injected intraperitoneally, observes and records small in 15min
Writhing response (belly indent, the stretching, extension hind leg, buttocks are raised) number of mouse simultaneously calculates each cell mean and suppresses percentage.
1.2.3 statistical method
Experimental data withRepresent, analyzed using the statistical softwares of spss 16.0, t is examined between carrying out group.
1.3 result
1.3.1 hot plate method result
Influence of the plant of table 1 to hot plate method in mice pain threshold
Note：Compared with blank control group, * P<0.05, * * P<0.01.
As shown in Table 1, significantly increased in the pain threshold of 30min, 45min and 60min polygonum aubertii Henry each group, with blank pair
More there is significant difference according to group, prompt former plant to have obvious analgesic activity to mice pain caused by thermostimulation.
As dosage increases it can be seen from polygonum aubertii Henry senior middle school low dose group, pain threshold is increase tendency.
Upon administration during 15min, in addition to positive controls pain threshold significantly increases, remaining each group pain threshold does not have
Too big change；Upon administration during 30min, positive controls pain threshold increasing option, and other each administration groups and blank control group
Compare, pain threshold significantly increases；Upon administration during 45min, positive controls pain threshold is begun to decline, and each administration group pain threshold
Lasting increase；When 60min is administered, positive controls pain threshold is without significant difference compared with blank control group, and remaining is given
Occur slightly declining when medicine group pain threshold is compared with 45min, but the still significant difference compared with blank control group.
1.3.2 writhing method result
The influence of the plant Dichlorodiphenyl Acetate induced mice writhing response of table 2
Note：Compared with blank control group, * P<0.05, * * P<0.01.
As shown in Table 2, the writhing number of aspirin group and polygonum aubertii Henry each group significantly reduces, compared with blank control group
There is significant difference, former plant has obvious analgesic activity to pain caused by chemical stimulation.
Polygonum aubertii Henry middle dose group inhibiting rate reaches highest 30.9%, and in each dosage group of polygonum aubertii Henry, middle dose group inhibiting rate is most
It is high.
2 plant anti-inflammatories are studied
According to《Herbal pharmacology research methodology》In requirement to heat-clearing medicine research method, dimethylbenzene is used in this experiment
Rat toes swelling inflammatory model caused by the small white mouse auricle edema inflammatory model and egg white of induction carries out body to Plant crude extract
Interior anti-inflammatory activity experiment.
2.1 material
2.1.1 animal
KM small white mouses, SPF levels, body weight (20 ± 2) g are purchased from Hunan SJA Laboratory Animal Co. , Ltd, and animal is closed
Lattice card number：SCXK (Hunan) 2013-0004), raise in Qinghai ethnic university pharmacological evaluation room, 22~26 DEG C of environment temperature, relatively
Humidity 55%~65%.SD rats, 180~200g of body weight, it is purchased from Hunan SJA Laboratory Animal Co. , Ltd, licensing
Number：SCXK (Hunan) 2013-0004), raise in Qinghai ethnic university pharmacological evaluation room, 22~26 DEG C of environment temperature, relative humidity
55%~65%.Animal drinking pure water animal drinking pure water, feed are that experimental mouse maintains particulate material, are purchased from Hunan Si Laike
Scape reaches experimental animal Co., Ltd, production card number：SCXK (Hunan) 2014-0002), adapt to environment and tested after one week, tested
Water 12h is can't help in preceding fasting.
2.1.2 medicine
Polygonum aubertii Henry stem extraction freeze-dried powder.Aspirin effervescent tablet (AstraZeneca pharmaceutical Co. Ltd, lot number
1401102).Dimethylbenzene (analysis is pure), physiological saline.Egg white (is purchased from Xining business section Jia Zhai markets).
2.1.3 instrument
2.2 method
2.2.1 mice caused by dimethylbenzene xylene ear swelling test
Healthy mice 110,18~22g of body weight is taken, is randomly divided into 11 groups, every group 10, blank control (physiological saline)
Group, positive control (aspirin) group, the high, medium and low dosage group of polygonum aubertii Henry.According to medicinal material 2~6g of usual amounts, this experiment is with oral
Crude drug amount 6g is calculated according to the reduction formula of experimental animal and people's dosage, obtained small as adult's (60kg) maximum consumption per day
The dosage (1.2g/Kg) of white mouse.If this is middle dose group, plus-minus sets up high dose and low dosage separately in this data.I.e. high agent
Amount group (2.4g/Kg), middle dose group (1.2g/Kg), low dose group (0.6g/Kg) blank control group gavage physiological saline；It is positive
Control group is administered by 0.4g/Kg dosage.
1 time/d of gastric infusion, continuous gavage 7d, after every group of mouse continuous gavage is administered 1 week, after last dose 1h, in each
Mouse auris dextra is two-sided to be coated with dimethylbenzene 0.1ml/ only, and left ear is not applied as control, is put to death after 1h, and it is wide to cut left and right helix, uses diameter
Auricle is taken respectively at left and right ear same position for 3mm card punch, is weighed, using the ear weight difference of left and right two as swelling, is calculated swollen
Swollen rate and swelling inhibiting rate.
2.2.2 egg causes pedal swelling experiment
Healthy SD rat 72, body weight 180g-220g is taken, is randomly divided into 12 groups, every group 6, blank control (physiology salt
Water) group, model group, positive control (aspirin) group, the high, medium and low dosage group of polygonum aubertii Henry.Foundation medicinal material 2~6g of usual amounts, this
Experiment is using oral crude drug amount 6g as adult's (60kg) maximum consumption per day, according to experimental animal and the reduction formula meter of people's dosage
Calculate, obtain the dosage (0.6g/Kg) of rat).If this is middle dose group, plus-minus sets up high dose and low dose separately in this data
Amount.That is high dose group (1.2g/Kg), middle dose group (0.6g/Kg), low dose group (0.3g/Kg).Blank control group gavage physiology
Salt solution；Positive controls are administered by 2mg/10g dosage.
1 times/day, continuous gavage 7d of gastric infusion, measured before last dose and recorded each with rat's foot volume measuring instrument
The left back sufficient volume of mouse.After last dose 30min, except blank group, egg is subcutaneously injected in rat right hind leg vola pedis respectively in remaining each group
Clear 0.1mL/ only causes inflammation.Then, in 1h, 2h, 3h, 4h, 5h, 6h with being measured with rat's foot volume measuring instrument and record right metapedes
Volume, so that the difference of vola pedis volume is swelling before and after inflammation, and calculates each cell mean and suppress percentage.
2.2.3 statistical method
Experimental data withRepresent, using the statistical softwares of spss 16.0, the t compare between group is examined.
2.3 result
2.3.1 mice caused by dimethylbenzene xylene ear swelling test
The influence of the small white mouse auricle edema of the paraxylene of table 3 induction
Note：Compared with blank control group, * P<0.05, * * P<0.01.
As shown in Table 3, aspirin group and polygonum aubertii Henry each group can play inhibitory action, wooden rattan to small white mouse auricle edema
More there is significant difference in knotweed high dose group and middle dose group, illustrate the induction of polygonum aubertii Henry paraxylene with blank control group
Small white mouse auricle edema has obvious antiinflammatory action.And antiinflammatory action is proportionate with dosage.
2.3.2 influence of the polygonum aubertii Henry to rat toes swelling caused by egg white
Influence of the polygonum aubertii Henry of table 4 to rat toes swelling caused by egg white
Note：Compared with blank control group, * P<0.05, * * P<0.01.
4 it can know that aspirin group and Radix Polygoni aubertii extract each group can play inhibitory action to rat toes swelling by table,
More there is significant difference in polygonum aubertii Henry high dose group, display polygonum aubertii Henry is lured egg white since 120min with blank control group
The rat toes swelling led has obvious antiinflammatory action.Each senior middle school's low dose group result of polygonum aubertii Henry, antiinflammatory action and dosage positive
Close.
The foregoing is only a preferred embodiment of the present invention, protection scope of the present invention not limited to this, any ripe
Those skilled in the art are known in the technical scope of present disclosure, the letter for the technical scheme that can be become apparent to
Altered or equivalence replacement are each fallen within protection scope of the present invention.
Claims (3)
1. a kind of research method of polygonum aubertii Henry analgesic and anti-inflammatory effects, it is characterised in that comprise the following steps：
Step 1, the influence to thermostimulation induced mice pain
(1) regulating thermostatic
Intelligent 55.0 ± 0.5 DEG C of hot-plate instrument design temperature, preheat 10min；
(2) qualified mouse is screened
(3) medicine effect is observed
Step 2, the influence to chemical stimulation induced mice pain
Healthy mice 110 is taken, 18~22g of body weight, every group 10, random point 11 groups, blank control physiological saline group is positive right
According to aspirin group, the high, medium and low dosage group of polygonum aubertii Henry；According to medicinal material 2~6g of usual amounts, this experiment is made with oral crude drug amount 6g
For the maximum consumption per day of 60kg adults, calculated according to the reduction formula of experimental animal and people's dosage, obtain the dosage of small white mouse
1.2g/Kg；If this is middle dose group, plus-minus sets up high dose and low dosage separately in this data；That is high dose group 2.4g/Kg, in
Dosage group 1.2g/Kg, low dose group 0.6g/Kg；Positive controls are administered by 0.4g/Kg, blank control group physiological saline gavage；
1 time a day, after continuous gavage 7d, then 0.2mL0.6% acetum is injected intraperitoneally, observes and records mouse in 15min
Writhing response belly indent, stretching, extension hind leg, buttocks raise number and calculate each cell mean and suppress percentage；
Step 3, mice caused by dimethylbenzene xylene ear swelling test
Healthy mice 110,18~22g of body weight is taken, is randomly divided into 11 groups, every group 10, blank control physiological saline group is positive
Compare aspirin group, the high, medium and low dosage group of polygonum aubertii Henry；According to medicinal material 2~6g of usual amounts, this experiment is with oral crude drug amount 6g
As adult's 60kg maximum consumption per days, calculated according to the reduction formula of experimental animal and people's dosage, obtain the medication of small white mouse
Measure 1.2g/Kg；If this is middle dose group, plus-minus sets up high dose and low dosage separately in this data；I.e. high dose group 2.4g/Kg,
Middle dose group 1.2g/Kg, low dose group 0.6g/Kg blank control group gavage physiological saline；Positive controls press 0.4g/Kg dosage
Administration；
1 time/d of gastric infusion, continuous gavage 7d, it is right in each mouse after last dose 1h after every group of mouse continuous gavage is administered 1 week
Ear is two-sided to be coated with dimethylbenzene 0.1ml/ only, and left ear is not applied as control, is put to death after 1h, and it is wide to cut left and right helix, with a diameter of 3mm
Card punch take auricle respectively at left and right ear same position, weigh, using the ear weight difference of left and right two as swelling, calculate swelling rate and
Swelling inhibiting rate；
Step 4, egg cause pedal swelling experiment
Healthy SD rat 72, body weight 180g-220g is taken, is randomly divided into 12 groups, every group 6, blank control physiological saline group, mould
Type group, positive control aspirin group, the high, medium and low dosage group of polygonum aubertii Henry；According to medicinal material 2~6g of usual amounts, this experiment is with oral
Crude drug amount 6g calculates according to the reduction formula of experimental animal and people's dosage as the maximum consumption per day of 60kg adults, obtains rat
Medication 0.6g/Kg；If this is middle dose group, plus-minus sets up high dose and low dosage separately in this data；That is high dose group 1.2g/
Kg, middle dose group 0.6g/Kg, low dose group 0.3g/Kg；Blank control group gavage physiological saline；Positive controls press 2mg/10g
Dosage is administered；
1 times/day, continuous gavage 7d of gastric infusion, is measured with rat's foot volume measuring instrument before last dose and to record each mouse left
Metapedes volume；After last dose 30min, except blank group, egg is subcutaneously injected in rat right hind leg vola pedis respectively in remaining each group
0.1mL/ only causes inflammation；Then, held in 1h, 2h, 3h, 4h, 5h, 6h with being measured with rat's foot volume measuring instrument and record right metapedes
Product, so that the difference of vola pedis volume is swelling before and after inflammation, and calculates each cell mean and suppress percentage；
Step 5, statistical method
Experimental data withRepresent, analyzed using the statistical softwares of spss 16.0, t is examined between carrying out group.
2. the research method of polygonum aubertii Henry analgesic and anti-inflammatory effects according to claim 1, it is characterised in that described in step 1
Screening qualified mouse is specially：
Healthy female mouse is taken, 18~22g of body weight, 1 is placed on hot-plate instrument every time, to place hot-plate instrument to occurring after mouse licks
S is the pain threshold of the mouse the time required to foot；Metapedes is licked in 5s or just after 30s or the mouse of jump abandons, select qualified mouse
110, every group 10, it is randomly divided into 11 groups, blank control physiological saline group, positive control aspirin group, polygonum aubertii Henry is high,
In, low dose group；Normal pain threshold before being administered after packet using 2 pain threshold average values as the mouse, and record.
3. the research method of polygonum aubertii Henry analgesic and anti-inflammatory effects according to claim 1, it is characterised in that described in step 1
Observe medicine effect
According to medicinal material 2~6g of usual amounts, this experiment is grown up maximum consumption per day using oral crude drug amount 6g as 60kg, is moved according to testing
Thing and the reduction formula of people's dosage calculate, and obtain the dosage 1.2g/Kg of small white mouse；If this is middle dose group, in this data
Upper plus-minus sets up high dose and low dosage separately；That is high dose group 2.4g/Kg, middle dose group 1.2g/Kg, low dose group 0.6g/Kg；Sun
Property control group by 0.4g/Kg be administered, blank control group physiological saline gavage；
By above-mentioned 1 time/d of dosage gastric infusion, continuous gavage 7d, 15 after last dose, 30,45,60min measure pain thresholds, if
The pain threshold of mouse is calculated more than 60s, its pain threshold with 60s after administration.
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| CN108553453A (en) * | 2018-06-06 | 2018-09-21 | 广西大学 | Spirulina extract anti-inflammatory effect studies the structure and application method of animal model |
| CN110353689A (en) * | 2019-07-19 | 2019-10-22 | 淄博职业学院 | A kind of writhing assay monitoring device |
| CN115282192A (en) * | 2022-09-13 | 2022-11-04 | 四川大学华西医院 | Composition with anti-inflammatory and analgesic effects, experiment and preparation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108553453A (en) * | 2018-06-06 | 2018-09-21 | 广西大学 | Spirulina extract anti-inflammatory effect studies the structure and application method of animal model |
| CN110353689A (en) * | 2019-07-19 | 2019-10-22 | 淄博职业学院 | A kind of writhing assay monitoring device |
| CN115282192A (en) * | 2022-09-13 | 2022-11-04 | 四川大学华西医院 | Composition with anti-inflammatory and analgesic effects, experiment and preparation method |
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