CN108553453A - Spirulina extract anti-inflammatory effect studies the structure and application method of animal model - Google Patents
Spirulina extract anti-inflammatory effect studies the structure and application method of animal model Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/01—Hydrocarbons
- A61K31/015—Hydrocarbons carbocyclic
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/748—Cyanobacteria, i.e. blue-green bacteria or blue-green algae, e.g. spirulina
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/0004—Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions
- A61K49/0008—Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
Abstract
The invention discloses the construction methods that a kind of spirulina extract anti-inflammatory effect studies animal model, i.e., the verification model of the acute swelling of mouse ear is established using dimethylbenzene as proinflammatory agent.Pass through IL 2, IL 6,1 β of IL, TNF α, IFN γ contents in detection model mice serum, record mice organs index, thymus gland, spleen histopathologic slide are made, the changes in microstructure of thymus gland and spleen from terms of tectology evaluates the antiphlogistic effects of spirulina extract.Research shows that, spirulina extract can promote the IL 2 of mouse, 6 levels of IL to a certain extent, and 1 β of IL for reducing mouse are horizontal, and being restored to normal level to TNF α, the IFN γ of mouse has certain positive effect, to inhibit relevant pathology damage process, reduce inflammation damage.Therefore, it can be utilized for the deep level development of spirulina using the present invention and clinical application provides foundation.
Description
Technical field
The invention belongs to spirulina development technique fields more particularly to a kind of spirulina extract anti-inflammatory effect to study animal
The structure and application method of model.
Background technology
Inflammation is a kind of defensive reaction of the animal body to pessimal stimulation, and sometimes, inflammatory reaction is to have for body
Benefit, but it is more when be harmful.Inflammatory reaction is the basis of many disease developments, with inflammatory reaction into
Row, many diseases start to occur, cause to damage to body.Therefore, anti-inflammatory effect is the emphasis side of many drug developments and research
One of to.Glucocorticoid is a kind of hormone medicine with powerful and rapid anti-inflammatory effect by acth secretion,
But there are the adverse reactions such as insanity, the immune, allergic rash of inhibition for glucocorticoid.In recent years, with Chinese medicine preparation
Further research and development, Chinese medicine preparation are also able to find and apply in the effect of anti-inflammatory aspect.The antioxidant activity of phycocyanin
Research is always the hot spot of its pharmacology activity research, many studies have shown that it is no matter in vivo or inside and outside, in spirulina extract
The phycocyanin contained can inhibit liposome peroxidation to react, and have antioxidation biology activity, the function of reducing inflammation.Spiral shell
Rotation algae extract has anti-inflammatory, anti-oxidant, enhancing because its abundant nutrition and good medical health effect receive significant attention
Be immunized and other effects, there is good research and development foreground.
Invention content
The technical problem to be solved in the present invention is to provide the structures that a kind of spirulina extract anti-inflammatory effect studies animal model
It builds and application method, solid reference is provided for the clinical development and application of spirulina extract anti-inflammatory effect.
In order to solve the above technical problems, the present invention uses following technical scheme：
Spirulina extract anti-inflammatory effect studies the construction method of animal model, and mouse ear is established by proinflammatory agent of dimethylbenzene
The verification model of acute swelling.
Above-mentioned construction method uniformly applies the dimethylbenzene for spreading 0.05mL with 1mL syringes on the left the ear portion two sides of mouse, waits for
There is ear swelling in mouse to obtain the final product.
The application method of spirulina extract anti-inflammatory effect research animal model obtained by above-mentioned construction method, including following step
Suddenly：
<1>Animal feeding and processing method
After the 70 cleaning grade Kunming mouse conventinal breedings 3 days for being 18-22g by average weight, it is randomly divided into 7 groups, it is female
Male fifty-fifty, setting blank control group, positive controls, 0.05% spirulina extract addition group, 0.1% spirulina extract add
Add group, 0.2% spirulina extract addition group, 0.3% spirulina extract addition group, 0.4% spirulina extract addition group,
It is used in conjunction 5 days；It respectively weighs before mouse medication and cause are scorching primary；
<2>Cause the foundation of scorching model；
<3>Observe influence of the spirulina extract to mice organs index；
<4>Detect IL-2, IL-6, IL-1 β, TNF-α, IFN-γ content.
Step<2>It is carried out by following operation：After latter hour is administered in last time, the left the ear portion two sides of mouse is noted with 1mL
Emitter uniformly applies the dimethylbenzene for spreading 0.05mL, waits for peacefully 20min, then uses the card punch of 6mm in mouse or so ear same position
Auricle is laid, is weighed rapidly, ear swelling degree, ear swelling rate and the swelling inhibiting rate of mouse are calculated.
Ear swelling degree, ear swelling rate and swelling inhibiting rate calculate as follows respectively：
Mice ear degree=left ear quality-auris dextra quality；
Ear swelling rate=(left ear quality-auris dextra quality)/auris dextra quality；
Swelling inhibiting rate=(blank group be averaged swelling-medication group be averaged swelling)/blank group be averaged swelling ×
100%.
Step<3>It is carried out by following operation：After causing inflammation and removing mouse auricle, mouse is dissected, visually observes its each tissue
Organ wins mouse liver, kidney and thymus gland and weighs, and records and calculates its each organ index.
Organ index calculates as follows：Organ index=each Organ weight/mouse weight.
Step<4>It is carried out by following operation：IL-2, IL-6, IL-1 β, TNF-α, IFN-γ content in mice serum are detected,
Thymus gland, spleen histopathologic slide are made, the changes in microstructure of thymus gland and spleen from terms of tectology evaluates spiral shell
Revolve the antiphlogistic effects of algae extract.
The problem of lacking effective animal model for spirulina extract anti-inflammatory effect, inventor establishes a kind of spirulina
Extract anti-inflammatory effect studies the construction method of animal model, i.e., establishes testing for the acute swelling of mouse ear by proinflammatory agent of dimethylbenzene
Model of a syndrome.The anti-inflammatory activity that spirulina extract can be inquired into using the model, to it, anti-inflammatory effect is studied in Mice Body.
IL-2, IL-6, IL-1 β, TNF-α, IFN-γ content specifically in detection model mice serum record mice organs index, system
Make thymus gland, spleen histopathologic slide, the changes in microstructure of thymus gland and spleen from terms of tectology evaluates spiral
The antiphlogistic effects of algae extract.Studies have shown that spirulina extract can promote IL-2, IL-6 water of mouse to a certain extent
Flat, the IL-1 β for reducing mouse are horizontal, and being restored to normal level to TNF-α, the IFN-γ of mouse has certain positive effect,
To inhibit relevant pathology damage process, reduce inflammation damage.Therefore, can be the deep level development of spirulina using the present invention
Using and clinical application provide foundation.
Description of the drawings
Fig. 1 is influence block diagram of the spirulina extract to mice ear inhibiting rate.
Fig. 2 is influence block diagram of the spirulina extract to mouse liver index.
Fig. 3 is influence block diagram of the spirulina extract to mouse kidney index.
Fig. 4 is influence block diagram of the spirulina extract to mouse spleen/thymus index.
Fig. 5 is influence block diagram of the spirulina extract to immunosuppressed mice and normal mouse IL-2 levels.
Fig. 6 is influence block diagram of the spirulina extract to immunosuppressed mice and normal mouse IL-6 levels.
Fig. 7 is influence block diagram of the spirulina extract to immunosuppressed mice and normal mouse IL-1 β levels.
Fig. 8 is influence block diagram of the spirulina extract to immunosuppressed mice and normal mouse TNF-α level.
Fig. 9 is influence block diagram of the spirulina extract to immunosuppressed mice and normal mouse INF- γ levels.
Figure 10 is influence block diagram of the spirulina extract to immunosuppressed mice and normal mouse index and spleen index.
Figure 11 is influence block diagram of the spirulina extract to immunosuppressed mice and normal mouse thymus index.
Figure 12 is blank control group spleen section figure (HE is dyed, × 20).
Figure 13 is blank control group spleen section figure (HE is dyed, × 40).
Figure 14 is immunosupress group spleen section figure (HE is dyed, × 20).
Figure 15 is immunosupress group spleen section figure (HE is dyed, × 40).
Figure 16 is 0.1% spirulina extract group spleen section figure (HE is dyed, × 20).
Figure 17 is 0.1% spirulina extract group spleen section figure (HE is dyed, × 40).
Figure 18 is 0.2% spirulina extract group spleen section figure (HE is dyed, × 20).
Figure 19 is 0.2% spirulina extract group spleen section figure (HE is dyed, × 40).
Figure 20 is 0.3% spirulina extract group spleen section figure (HE is dyed, × 20).
Figure 21 is 0.3% spirulina extract group spleen section figure (HE is dyed, × 40).
Figure 22 is blank control group thymus section figure (HE is dyed, × 10).
Figure 23 is immunosupress group thymus section figure (HE is dyed, × 10).
Figure 24 is 0.1% spirulina extract group thymus section figure (HE is dyed, × 10).
Figure 25 is 0.2% spirulina extract group thymus section figure (HE is dyed, × 10).
Figure 26 is 0.3% spirulina extract group thymus section figure (HE is dyed, × 10).
Specific implementation mode
It further illustrates the present invention and how to implement by the following examples.Wherein, spirulina extract powder (could be brief
Description preparation method provides preparation method source) it is provided by the North Sea bio tech ltd Sheng Bada, aquatic organism research institute of Chinese Academy of Sciences prison
System.
Spirulina extract powder preparation method is as follows：Spirulina powder is weighed, sodium pyrophosphate solution broken wall is added；Adjust algae powder liquid
PH=10,80 DEG C of water-bath 8h adjust algae powder liquid pH=5, and papain is added, after 50 DEG C act on 1.5h, by enzyme-deactivating, finally
In 80 DEG C of water-bath 3h；95% ethyl alcohol, precipitates overnight is added in centrifugation, supernatant concentration, and supernatant is abandoned in centrifugation, precipitates anhydrous second
Alcohol, acetone, ether washing, it is dry, it grinds to get spirulina extract powder.
1 animal feeding of embodiment and processing method
After the 70 cleaning grade Kunming mouse conventinal breedings 3 days for being 18-22g by average weight, 7 groups are randomly divided into, often
Group 10, half male and half female, setting blank control group, positive controls, spirulina extract addition group (0.05%, 0.1%,
0.2%, 0.3%, 0.4%).Specific grouping and processing scheme are shown in Table 1.
Table 1 is grouped and processing scheme
Embodiment 2 causes the foundation of scorching model
It respectively weighs before mouse medication and cause are scorching primary.After latter hour is administered in last time, to the left the ear portion of mouse two
0.05 milliliter of dimethylbenzene is spread in face with the uniform painting of 1mL syringes, waits for peacefully 20min.Then use the card punch of 6mm in mouse or so
Ear same position lays auricle, weighs rapidly.Calculate ear swelling degree, ear swelling rate and the swelling inhibiting rate of mouse.Each calculating side
Method is as follows：
Mice ear degree=left ear quality-auris dextra quality；
Ear swelling rate=(left ear quality-auris dextra quality)/auris dextra quality；
Swelling inhibiting rate=(blank group be averaged swelling-medication group be averaged swelling)/blank group be averaged swelling ×
100%.
Influence of the spirulina extract to ear swelling test mice ear swelling situation the results are shown in Table 2, Fig. 1.
2 spirulina extract of table influences result to ear swelling test mice ear swelling
As shown in Table 2 and Fig. 1, the ear swelling degree of each dose helical algae extract addition group mouse and its ear swelling rate are with sky
White control group comparing difference is not notable, and 0.3% dose helical algae extract addition group ear swelling inhibiting rate is higher than positive controls.
The result shows that ear skin eye sees each group difference and does not have conspicuousness after medication.Spirulina extract addition is raised
Expect that experimental group increasing with additive capacity, ear swelling inhibiting rate have apparent rising, is added in 0.3% spirulina extract
Group reaches peak, and 5.488% far more than dexamethasone positive controls reaches 19.43%.Spirulina extract is herein
The ability of powerful anti-mouse ear swelling is shown in experiment, wherein the ear swelling suppression of 0.3% spirulina extract addition group
Rate processed is even better than the effect of positive control drug.
Embodiment 3 observes influence of the spirulina extract to mice organs index
After causing inflammation and removing mouse auricle, mouse is dissected, its each histoorgan is visually observed, wins mouse liver, kidney
And thymus gland is weighed, and is recorded and is calculated its each organ index.Computational methods are as follows：
Organ index=each Organ weight/mouse weight.
Influence result of 3 spirulina extract of table to mice organs index
Note：" * * " is indicated compared with blank control group, and difference is extremely significantly (P ＜ 0.01).Similarly hereinafter.
As shown in table 3 and Fig. 2-Fig. 4, positive controls liver index is compared with blank control group, and difference pole significantly rises
(P ＜ 0.01), each dose helical algae extract addition group be not notable with blank control group difference.
The result shows that the spirulina extract addition liver index of feed, renal index, index and spleen index, thymus index with
Blank control group is compared to there are no significant difference.Dexamethasone compares liver index as the control group of positive drug with blank group
It extremely significantly increases (P ＜ 0.01), spleen and thymus index and is remarkably decreased (P ＜ 0.01).Spleen and thymus gland are animal body weights
Immune organ is wanted, and under the action of dexamethasone, mouse thymus and the equal conspicuousness atrophy of spleen affect the immune work(of body
Energy.At the same time, dexamethasone improves mouse liver index, it is shown that dexamethasone can cause hepatomegaly, be brought to liver negative
Face is rung.Dexamethasone belongs to glucocorticoid medicine, has stronger anti-inflammatory and immunosuppressive effect, but long-term a large amount of
Using may result in some adverse reactions, in this experimental study, dexamethasone causes mouse liver enlargement, spleen and thymus gland
Atrophy.And spirulina extract antiphlogistic effects compared with dexamethasone are more preferable, have no adverse reaction, to the liver kidney and thymus gland of mouse
Spleen is substantially without influence.
Embodiment 4 detects IL-2, IL-6, IL-1 β, TNF-α, IFN-γ content
80 SPF grades of Kunming mouses are randomly divided into 8 groups, every group 10, half male and half female.It is carried out after mouse adapts to 3d
Formal experiment.Each group processing method is as shown in table 4.
4 each group processing method of table
Period observes sooner or later the diet of mouse and cerebration situation twice daily.In experiment the 8th day, i.e., after the last administration
Next day weighs to all mouse.Eyeball is taken a blood sample, and serum is centrifuged after static, and -80 DEG C of packing freezes spare.According to
ELISA kit illustrates to detect IL-2, IL-6, IL-1 β, TNF-α, IFN-γ content in serum.
Influence result of 5 spirulina extract of table to immunosuppressed mice and normal mouse IL-2, IL-6, IL-1 β level
As shown in table 5 and Fig. 5-Fig. 7, each group IL-2, IL-6, IL-1 β level do not have with blank control group compared to difference
It is significant.
IL-2 can both promote lymphopoiesis, enhance body's immunity, and energy restricted T cells react and enhance machine
The immune tolerance of body, therefore can be used for treating tumour and infectious diseases and autoimmune disease.The result shows that positive controls
Serum IL-2 level be slightly less than blank control group, and spirulina extract addition group has promotion by a small margin, reaches normal
Level illustrates that spirulina extract promotes the recovery of immunosuppressed mice IL-2 to a certain extent, can promote the IL- of normal mouse
2 is horizontal.
IL-6 is a kind of multi-functional inflammatory cytokine, is the key element in inflammatory mediator network, in inflammatory reaction
In play double regulation control.As a kind of anti-inflammatory cytokines, IL-6 can balance inflammatory cytokine or earlier damage
Effect.IL-6 can protect cell in experimental lung damage test by inhibiting macrophage to generate IL-1 and tumor necrosis factor
In the damage that is subject to.For IL-6 with inflammation and anti-inflammatory two-way regulating function is caused, effect is close with the content in tissue
It is relevant.The result shows that the IL-6 levels of positive controls are less than blank control group, regardless of being immunosuppressed mice or just
Its IL-6 level of the spirulina extract addition group of normal mouse feeding is close to blank control group.But may due to medication cycle compared with
Short, function and effect play slowly, and variation is not notable.This research illustrates that spirulina extract can promote to exempt to a certain extent
Epidemic disease inhibits the IL-6 of mouse to be restored to normal level.
IL-1 β are a kind of important proinflammatory cytokine, many pathologic processes of wide participation, such as the destruction of body tissue, water
Swollen formation etc..The result shows that the immunosuppressed mice of feeding spirulina extract addition feed, IL-1 β levels are omited
Less than blank control group and positive controls, and feed the IL-1 β water steadyings of the normal mouse of spirulina extract addition feed
It is fixed, it is close with blank control group.But since medication cycle is shorter, function and effect play slowly, and variation is not notable.This experiment
The IL-1 β levels of immunosuppressed mice can be reduced by illustrating spirulina extract to a certain extent, to inhibit relevant pathology
Damage process.
Influence result of 6 spirulina extract of table to immunosuppressed mice and normal mouse TNF-α and INF- γ levels
As shown in table 6 and Fig. 8-Fig. 9, each group TNF-α is compared difference with blank control group with INF- γ levels and is not had
Conspicuousness.
TNF-α is a kind of general biological activity cell factor generated by multinuclear macrophage.It can keep tumour cell bad
Extremely, the immune defence line of body is formed, while can also the pathologic-physiological reactions such as transmitting inflammation process, tissue damage, shock.As a result table
Bright spirulina extract is restored to normal level to the TNF-α level of immunosuppressed mice has certain effect.
IFN-γ is a kind of antiviral and antitumor and carries out immunoregulatory cell factor, mainly by T cell and NK cells
It is generated after activation, it is strong to the immunoloregulation function of body.The result shows that positive controls level is less than blank control group, and
The immunosuppressed mice IFN-γ level of feeding spirulina extract addition feed is restored to normal level, and wherein spirulina carries
Take 0.1% dosage of object and 0.2% dosage of spirulina extract even a little higher than blank control group.This explanation, spirulina extract
To the IFN-γ of immunosuppressed mice be restored to normal level have the function of it is certain positive.
Embodiment 5
After the last administration, cervical dislocation puts to death mouse, takes out thymus gland and spleen is weighed, and calculate organ index.By thymus gland,
Spleen is immersed in formalin fixer and fixes 7d, does histotomy, observes the histological change of thymus gland and spleen.
Influence of 7 spirulina extract of table to immunosuppressed mice and normal mouse organ index
As shown in table 7 and Figure 10-Figure 11, the spleen of cyclophosphamide positive controls and each dosage medication group of cyclophosphamide refers to
Number declines, extremely significantly (P ＜ 0.01) compared to difference with blank control group, wherein 0.2% dose helical algae extract addition group pair
The index and spleen index of immunosupress and normal mouse has a certain upgrade effect, but difference is not notable.
Observation each group spleen histopathologic slide can see：Blank control group (Figure 12, Figure 13) spleen white pulp, red pulp knot
Structure is clear, acini lienalis structural integrity, lymphocyte comparatively dense；The white red pulp boundary of immunosupress group (Figure 14, Figure 15) is unintelligible, spleen
Minibody structure is fuzzy, and atrophy, also can be observed lymphocyte and desmacyte degeneration necrosis, lymphocyte are sparse；Medication group
Phase (Figure 16-Figure 21) is compared with positive controls, and acini lienalis structure is more complete, lymphocyte comparatively dense, while with drug agent
The increase of amount, macrophage also increase therewith.This illustrates that spirulina extract can enhance the reparation work(of immunosuppressed mice spleen
Can, pick up anti-immunity inhibiting effect with certain.
Observation each group thymus gland histopathologic slide can see：Blank group (Figure 22) skin medullary substance is high-visible, medullary substance area chest
Gland minibody structure is clear；The boundary of positive controls (Figure 23) skin medullary substance is fuzzy, and lymphocyte is reduced, and Hassal's body substantially reduces；
Medication group (Figure 24-Figure 26) corresponding positive control group, the boundary of skin medullary substance start to be apparent from, and lymphocyte starts to increase, thymus gland
Corpusculum starts to increase, while the increase of visible macrophage.This explanation, spirulina extract damage the thymus gland of immunosuppressed mice
Wound has certain repair function, has certain curative effect to picking up anti-immunity inhibiting effect.
Conclusion：The present invention demonstrates spirulina extract anti-inflammatory efficacy by building animal model, can be that spirulina extracts
Establish good Research foundation in the clinical application direction of object.
Claims (8)
1. a kind of construction method of spirulina extract anti-inflammatory effect research animal model, it is characterised in that scorching to cause with dimethylbenzene
The verification model of the acute swelling of mouse ear is established in agent.
2. the construction method of spirulina extract anti-inflammatory effect research animal model according to claim 1, feature exist
In uniformly applying the dimethylbenzene for spreading 0.05mL with 1mL syringes on the left the ear portion two sides of mouse, wait for that mouse ear swelling occurs to obtain the final product.
3. the application method of spirulina extract anti-inflammatory effect research animal model obtained by construction method described in claim 1,
It is characterized in that including the following steps：
<1>Animal feeding and processing method
After the 70 cleaning grade Kunming mouse conventinal breedings 3 days for being 18-22g by average weight, 7 groups are randomly divided into, male and female are each
Half, setting blank control group, positive controls, 0.05% spirulina extract addition group, the addition of 0.1% spirulina extract
Group, 0.2% spirulina extract addition group, 0.3% spirulina extract addition group, 0.4% spirulina extract addition group, even
With 5 days；It respectively weighs before mouse medication and cause are scorching primary；
<2>Cause the foundation of scorching model；
<3>Observe influence of the spirulina extract to mice organs index；
<4>Detect IL-2, IL-6, IL-1 β, TNF-α, IFN-γ content.
4. the application method of spirulina extract anti-inflammatory effect research animal model according to claim 1, feature exist
In step<2>It is carried out by following operation：After latter hour is administered in last time, the left the ear portion two sides of mouse is equal with 1mL syringes
It is even to apply the dimethylbenzene for spreading 0.05mL, 20min is waited for peacefully, then lays ear in mouse or so ear same position with the card punch of 6mm
Piece is weighed rapidly, and ear swelling degree, ear swelling rate and the swelling inhibiting rate of mouse are calculated.
5. the application method of spirulina extract anti-inflammatory effect research animal model according to claim 1, feature exist
It is calculated as follows respectively in the ear swelling degree, ear swelling rate and swelling inhibiting rate：
Mice ear degree=left ear quality-auris dextra quality；
Ear swelling rate=(left ear quality-auris dextra quality)/auris dextra quality；
Swelling inhibiting rate=(blank group be averaged swelling-medication group be averaged swelling)/blank group is averaged swelling × 100%.
6. the application method of spirulina extract anti-inflammatory effect research animal model according to claim 1, feature exist
In step<3>It is carried out by following operation：After causing inflammation and removing mouse auricle, mouse is dissected, its each histoorgan is visually observed, plucks
It takes mouse liver, kidney and thymus gland to weigh, record and calculates its each organ index.
7. the application method of spirulina extract anti-inflammatory effect research animal model according to claim 1, feature exist
It is calculated as follows in the organ index：Organ index=each Organ weight/mouse weight.
8. the application method of spirulina extract anti-inflammatory effect research animal model according to claim 1, feature exist
In step<4>It is carried out by following operation：IL-2, IL-6, IL-1 β, TNF-α, IFN-γ content in mice serum are detected, chest is made
Gland, spleen histopathologic slide, the changes in microstructure of thymus gland and spleen from terms of tectology, evaluation spirulina carry
Take the antiphlogistic effects of object.
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Cited By (1)
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| CN115666610A (en) * | 2020-05-19 | 2023-01-31 | 瓦克萨科技有限公司 | Photosynthetic controlled spirulina extracts for the treatment of cytokine storm syndrome |
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Application publication date: 20180921 |