CN115282087A - Nanometer gold oil peptide capable of permeating to dermis layer and preparation method and application thereof - Google Patents
Nanometer gold oil peptide capable of permeating to dermis layer and preparation method and application thereof Download PDFInfo
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- CN115282087A CN115282087A CN202210942147.7A CN202210942147A CN115282087A CN 115282087 A CN115282087 A CN 115282087A CN 202210942147 A CN202210942147 A CN 202210942147A CN 115282087 A CN115282087 A CN 115282087A
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- gold oil
- nano gold
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- 239000013589 supplement Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 229960001727 tretinoin Drugs 0.000 description 1
- 229940099259 vaseline Drugs 0.000 description 1
- 235000019871 vegetable fat Nutrition 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 230000037373 wrinkle formation Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/06—Emulsions
- A61K8/068—Microemulsions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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Abstract
The invention relates to the technical field of cosmetics, in particular to a nano gold oil peptide capable of permeating to a dermis layer, a preparation method and application thereof, wherein the preparation method comprises the steps of compounding raw materials of the nano gold oil peptide, and treating the raw materials by adopting a high-pressure microjet homogenization method to obtain the nano gold oil peptide; wherein the nano gold oil peptide comprises the following raw materials in parts by weight: 30-50 parts of adductor seed oil, 30-50 parts of synthetic esters or alkanes, 0.1-1.0 part of small molecular peptide, 0.1-0.5 part of dimethyl methoxy chromanol, 0.04-0.06 part of ceramide NP, 1-5 parts of humectant, 10-20 parts of polyglycerol emulsifier and 1.9-2.1 parts of additive. The nano gold oil peptide in the form of nano liposome is prepared from the composition with specific components and proportions by a high-pressure micro-jet homogenization method, has the effects of efficient permeation, water solubility, good compatibility, nutrition supplementation, moisture preservation promotion, barrier repair, oxidation resistance, free radical resistance and the like, and can play the effects of tightening and wrinkle resistance in all directions.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to a nano gold oil peptide capable of permeating into a dermis layer, and a preparation method and application thereof.
Background
With the increasing reliance on 'nourishing skin with oil' and 'oil + peptide' innovative skin care, natural vegetable oil becomes an upgrade of essence, and the demand of anti-aging products is increased along with the aging of the population. Aging is an irresistible natural process of life, skin aging is the result of the combined action of internal and external causes, and laxity and wrinkles are the signs of the appearance of skin aging. With the age, the moisture in the skin is reduced, the collagen fiber becomes thin, the elasticity of the elastin is reduced, the collagen and the elastin in the dermis are interacted to form a regular network structure and are gradually disintegrated, and finally, the generation of wrinkles is caused.
In view of the wrinkle formation principle and process, anti-wrinkle active ingredients commonly used in cosmetics mainly include active ingredients that promote skin moisturization and repair skin barrier function. The vegetable oil contains polyunsaturated fatty acids which are indispensable to human bodies, the fatty acids have the functions of keeping moisture, moistening skin, resisting free radicals and improving the antioxidant capacity of the skin, meanwhile, most of the vegetable oil contains natural active substances, the texture is light, the fluidity is good, the vegetable oil is suitable for various types of skin, and the development of safe and mild efficacy cosmetics by utilizing the vegetable oil is a hotspot and a trend in the current cosmetic industry.
The Indian fruit is a plant growing in the Amazon river basin, is well known by local people as early as thousands of years, and can completely retain the precious biological activity, trace elements and the like of the Mei Guo by adopting a hydraulic cold pressing method. In addition, the semen Plukenetia volubilis contains natural minerals and natural vitamins, and the total content of vitamin E in the oil of Plukenetia volubilis can reach 190mg/100g, and is a very good natural antioxidant, which is rare for other vegetable oils and fats.
In the prior art, the crabapple oil is usually compounded with small molecular peptides, chromanol, ceramide NP and the like, and the antioxidant cosmetics are prepared by adopting a direct mixing mode. However, the cosmetics prepared by the method generally have low solubility of small molecular peptides, ceramide NP and the like, cannot be uniformly dispersed in a water system, and further, the adductor oil cannot be well fused with the small molecular peptides, the ceramide NP and the like, but exists in a suspension or emulsion state, so that the compounding effect is poor. Therefore, the mixture obtained by the traditional mixing mode has the limitation of use, can be dissolved only in an oily system, and is difficult to apply in an aqueous system.
Therefore, in view of the above problems, the development of a novel nano gold oil peptide capable of permeating into the dermis layer, and a preparation method and application thereof are a technical problem to be solved by those skilled in the art.
Disclosure of Invention
The invention aims to provide a preparation method of nano gold oil peptide capable of permeating to a dermis layer, which has the advantages of simple operation, high bioavailability, wide application range and the like;
the second purpose of the invention is to provide a nano gold oil peptide which can permeate to the dermis, has the effects of high-efficiency permeation, water solubility, good compatibility, nutrition supplementation, moisture promotion, barrier repair, oxidation resistance, free radical resistance and the like, and can play the effects of tightening and wrinkle resistance in all directions.
The invention provides a preparation method of nano gold oil peptide capable of permeating to a dermis layer, which comprises the steps of compounding raw materials of the nano gold oil peptide, and treating by adopting a high-pressure microjet homogenization method to obtain the nano gold oil peptide;
wherein the nano gold oil peptide comprises the following raw materials in parts by weight: 30-50 parts of adductor seed oil, 30-50 parts of synthetic esters or alkanes, 0.1-1.0 part of small molecular peptide, 0.1-0.5 part of dimethyl methoxy chromanol, 0.04-0.06 part of ceramide NP, 1-5 parts of humectant, 10-20 parts of polyglycerol emulsifier, 1.9-2.1 parts of additive and 2-5 parts of water.
The nano gold oil peptide consists of a plurality of different raw materials, wherein:
adding the fruit seed oil: contains natural minerals and natural vitamins of human body, and the total content of vitamin E can reach 190mg/100g, and is a good natural antioxidant;
the micromolecular peptide and the dimethyl methoxy chromanol serve as main components for resisting oxidative damage, have great potential for removing free radicals and resisting oxidation, and can effectively solve the problems of pigmentation, skin aging and even canceration;
ceramide NP is used as barrier repair component, and can rapidly recover skin moisture retention and barrier function.
In addition, the nano gold oil peptide is prepared by using a high-pressure micro-jet homogenization method, so that the particle size of the preparation can be effectively reduced, and the preparation can reach a nano level. Therefore, an effective component delivery system in cosmetics is conveniently realized by using a micro-nano preparation-based effective component encapsulation mode, and the problems of instability, large peculiar smell, difficult compatibility, difficult skin absorption and the like in the application process of the effective components are solved.
According to the high-pressure micro-jet homogenizing method, under extremely high shearing impact force, smaller and more uniform particle size distribution is obtained, the delivery process of various active substances can be met, the using amount of expensive materials is effectively reduced, and the high-pressure micro-jet homogenizing method has better appearance, skin feel and penetrating absorbability. Research shows that the nano golden oil peptide can penetrate 15 layers of the epidermis layer (tested by a tape method), namely, the nano golden oil peptide can penetrate through the horny layer to the true cortex.
The synthetic esters mainly comprise any one or two of caprylic/capric triglyceride and cetyl ethyl hexanoate; the alkane mainly comprises any one or two of isomeric hexadecane and C9-12 alkane; the small molecule peptide comprises the following raw materials in percentage by weight: palmitoyl tripeptide-1.004-0.006 part, palmitoyl pentapeptide-4.004-0.006 part and acetyl octapeptide-3.1-1.0 part.
The additive specifically comprises the following raw materials in parts by weight: 0.04-0.06 part of phytosterol oleate, 2.0-4.0 parts of hydrogenated polyisobutene, 0.1-0.5 part of isosorbide dimethyl ether, 0.1-1.0 part of tocopherol and 0.08-0.2 part of rhizoma anemarrhenae root extract, wherein the content of the rhizoma anemarrhenae sapogenin in the rhizoma anemarrhenae root extract is 10 mu M-20 mu M;
the polyglycerol emulsifier comprises one or more of polyglycerol-2 caprate, polyglycerol-6 caprylate, polyglycerol-2 sesquioleate, polyglycerol-10 dioleate and polyglycerol-6 didecanate;
the humectant includes any one of glycerin, butylene glycol, and 1, 2-hexanediol.
Wherein, the phytosterol oleate is a vaseline component and is used as a skin conditioner in the nanometer gold oil peptide;
the hydrogenated polyisobutene has the cosmetic characteristics of natural squalane, is moist and not greasy, can keep moisture and is smooth, and has strong permeability, good thermal stability and good storage stability;
the dimethyl isosorbide anhydride is mainly used as a solvent and a viscosity reducer, can dissolve free fat in the skin to penetrate through a skin membrane, and further enhances the conveying capacity of other components in a cosmetic formula; in addition, the dissolving effect of the skin whitening cream can enable the skin whitening cream to effectively act on skin disease sources, and further enhance the skin whitening and oxidation resistance.
The rhizoma anemarrhenae extract has certain inhibiting and killing effects on various virus and bacteria, has anti-inflammation effect, and can be used for preventing and treating dermatitis.
In addition, in order to further improve the antioxidant effect of the nano gold oil peptide, the used vegetable oil contains 1-2 parts of sesame seed extract besides the seed oil of the Indian fruit.
Preferably, in the technical scheme, when the high-pressure micro-jet flow is homogenized, the pressure is controlled to be 20-300MPa, the temperature is controlled to be 25-35 ℃, and the homogenizing time is 4-6min.
Preferably, the technical scheme specifically comprises the following steps:
s1, mixing and stirring water, a humectant, palmitoyl tripeptide-1, palmitoyl pentapeptide-4 and acetyl octapeptide-3 until the mixture is dissolved and transparent to obtain a phase A mixed solution;
s2, mixing and stirring the rest components until the rest components are dissolved to obtain a B-phase mixed solution;
s3, adding the A-phase mixed solution into the B-phase mixed solution, and continuously stirring to obtain an emulsion;
s4, carrying out high-pressure micro-jet homogenization treatment on the emulsion obtained in the step S3 to obtain nano gold oil peptide;
wherein, the steps S1 and S2 have no requirement of the sequence.
In the present invention, in order to improve the preparation efficiency of the B-phase mixed solution, the remaining components may be mixed and heated to 75-85 ℃, and then stirred and dissolved to obtain the B-phase mixed solution, but the temperature of the present invention is not particularly limited.
Preferably, in step S3, after the temperature of the phase B mixed solution is reduced to room temperature, the phase a mixed solution is added to the phase B mixed solution.
The invention also discloses the nano gold oil peptide prepared by the preparation method, and the nano gold oil peptide also belongs to the protection scope of the invention.
Preferably, in the technical scheme, the nano gold oil peptide is nano emulsion with the particle size of 100-1000 nm.
In addition, the application of the nanometer gold oil peptide prepared by the preparation method in preparing skin care products also belongs to the protection scope of the invention.
The preparation method of the nanometer gold oil peptide capable of permeating into the dermis at least has the following technical effects:
1. in order to better exert the anti-wrinkle effect of the plant seed oil of the crataegus pinnatifida bunge, the micromolecular peptide, the ceramide NP and the like, the nano gold oil peptide in the form of nano liposome is prepared from the composition with specific components and proportions by a high-pressure microjet homogenization method, can permeate into the true cortex, has the effects of high-efficiency permeation, water solubility, good compatibility, nutrition supplementation, moisture promotion, barrier repair, oxidation resistance, free radical resistance and the like, and can comprehensively exert the effects of tightening and anti-wrinkle;
2. according to the nano lipid oil peptide prepared by the high-pressure micro-jet homogenization method, the interfacial tension between the oil phase and the water phase becomes very small, so that the obtained emulsion particles are uniformly dispersed, the nano emulsion is characterized by high stability and good absorbability. Therefore, the preparation method has the advantages of simple operation, high bioavailability, wide application range and the like, obviously increases the solubility of vegetable oil, ceramide NP and the like in water, can add small molecular peptides into oily products, and has potential market application value;
3. the nano gold oil peptide comprises components such as crabapple seed oil, small molecular peptide, dimethyl methoxy chromanol, ceramide NP, an emulsifier, an additive and the like, wherein the total content of vitamin E in the crabapple oil can reach 190mg/100g, and the nano gold oil peptide is a very good natural antioxidant; the micromolecular peptide and the dimethyl methoxy chromanol serve as main components for resisting oxidative damage, have great potential for removing free radicals and resisting oxidation, and can effectively solve the problems of pigmentation, skin aging and even canceration; and the ceramide NP is used as a barrier repair component and can quickly restore the functions of skin moisture retention and barrier. The invention adopts a high-pressure micro-jet homogeneous preparation method, and can prepare the Indian fruit seed oil, the small molecular peptide, the ceramide NP, the emulsifier and the like into a transparent solution system with low viscosity, thermodynamic stability and the grain diameter of 100-1000nm according to a proper proportion.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a particle size distribution diagram of the nano-gold oil peptide prepared in example 3 of the present invention.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an", and "the" include plural forms as well, unless the context clearly indicates otherwise, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of the stated features, steps, operations, devices, components, and/or combinations thereof.
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Each 1 part in the following examples and comparative examples means 10g.
Example 1
The preparation method of the nanometer gold oil peptide comprises the following steps;
s11, preparing A-phase mixed solution
Composition of phase A mixture: 2 parts of water, 5 parts of glycerol, 0.004 part of palmitoyl tripeptide, 0.004 part of palmitoyl pentapeptide and 0.2 part of acetyl octapeptide.
Stirring the mixture of the components until the components are dissolved and transparent to obtain an A-phase mixed solution;
s12, preparing a B phase mixed solution
Composition of the B phase mixture: 30 parts of crassula indica seed oil, 0.1 part of dimethyl methoxy chromanol, 0.04 part of ceramide NP, 10 parts of polyglycerol-2 decanoate, 0.04 part of phytosterol oleate, 2.0 parts of hydrogenated polyisobutene, 0.2 part of isosorbide dimethyl ether, 0.1 part of tocopherol, 0.08 part of rhizoma anemarrhenae root extract (the content of the timosaponin is 20 mu M), 1 part of sesame seed extract and 30 parts of caprylic/capric triglyceride.
Heating the mixture of the components to 75 ℃, and stirring until the components are dissolved to obtain a B-phase mixed solution;
s13, preparing emulsion
After the temperature of the B-phase mixed solution is reduced to room temperature, adding the A-phase mixed solution into the B-phase mixed solution, and continuously stirring to obtain an emulsion;
s14, preparing nano gold oil peptide
Injecting the obtained emulsion into a high-pressure micro-jet homogenizer through a booster pump, and simultaneously controlling the pressure to be 100MPa, the temperature to be 25 ℃ and the homogenizing time to be 6min to obtain the nano-gold oil peptide.
Example 2
The preparation method of the nano gold oil peptide comprises the following steps;
s21, preparing A-phase mixed solution
Composition of phase A mixture: 5 parts of water, 5 parts of butanediol, 0.006 part of palmitoyl tripeptide, 0.006 part of palmitoyl pentapeptide and 1.0 part of acetyl octapeptide.
Stirring the mixture of the components until the components are dissolved and transparent to obtain an A-phase mixed solution;
s22, preparing a B-phase mixed solution
Composition of the B phase mixture: 50 parts of crabapple seed oil, 0.5 part of dimethyl methoxy chromanol, 0.06 part of ceramide NP, 20 parts of polyglycerol-2 sesquioleate, 0.06 part of phytosterol oleate, 4.0 parts of hydrogenated polyisobutene, 0.5 part of isosorbide dimethyl ether, 1.0 part of tocopherol, 0.2 part of rhizoma anemarrhenae root extract (the content of the rhizoma anemarrhenae sapogenin is 20 mu M), 2 parts of sesame seed extract and 35 parts of isomeric hexadecane.
Heating the mixture of the components to 85 ℃, and stirring until the components are dissolved to obtain a B-phase mixed solution;
s23, preparing emulsion
After the temperature of the mixed solution of the phase B is reduced to room temperature, adding the mixed solution of the phase A into the mixed solution of the phase B, and continuously stirring to obtain emulsion;
s24, preparing nano gold oil peptide
And injecting the obtained emulsion into a high-pressure micro-jet homogenizer through a booster pump, and simultaneously controlling the pressure to be 300MPa, the temperature to be 35 ℃ and the homogenizing time to be 4min to obtain the nano golden oil peptide.
Example 3
The preparation method of the nano gold oil peptide comprises the following steps;
s31, preparing A phase mixed solution
Composition of phase A mixture: 5 parts of water, 2 parts of glycerol, 2 parts of 1, 2-hexanediol, 0.005 part of palmitoyl tripeptide-1, 0.005 part of palmitoyl pentapeptide-4 and 0.5 part of acetyl octapeptide-3.
Stirring the mixture of the components until the components are dissolved and transparent to obtain A-phase mixed liquor;
s32, preparing a B-phase mixed solution
Composition of the B phase mixture: 40 parts of crabapple seed oil, 0.3 part of dimethyl methoxy chromanol, 0.05 part of ceramide NP, 3 parts of polyglycerol-6 caprylate, 5 parts of polyglycerol-2 sesquioleate, 0.05 part of phytosterol oleate, 3.0 parts of hydrogenated polyisobutene, 0.3 part of isosorbide dimethyl ether, 0.5 part of tocopherol, 0.1 part of rhizoma anemarrhenae root extract (the content of the timosaponin is 20 mu M), 1.5 parts of sesame seed extract and 30 parts of cetyl ethyl hexanoate.
Heating the mixture of the components to 80 ℃, and stirring until the components are dissolved to obtain a B-phase mixed solution;
s33, preparing emulsion
After the temperature of the mixed solution of the phase B is reduced to room temperature, adding the mixed solution of the phase A into the mixed solution of the phase B, and continuously stirring to obtain emulsion;
s34, preparation of nano gold oil peptide
And injecting the obtained emulsion into a high-pressure micro-jet homogenizer through a booster pump, and simultaneously controlling the pressure to be 150MPa, the temperature to be 30 ℃ and the homogenizing time to be 5min to obtain the nano gold oil peptide.
Comparative example 1
Composition of phase A mixture: 5 parts of water, 15 parts of glycerol, 0.005 part of wheat peptide and 0.005 part of oat peptide.
The rest is substantially the same as in example 3.
Comparative example 2
In the comparative example, the mixed solution of phase B was not added with the extract of kojiki root and dimethylmethoxychromanol.
Otherwise, it is substantially the same as in example 3.
Comparative example 3
The procedure was substantially the same as in example 3, except that the apparatus used for homogenization was different.
In this comparative example, an intermittent high-shear dispersion emulsifying homogenizer was used, and the homogenizing conditions were as follows: the temperature is 30 deg.C, and the homogenizing time is 10min.
Test example 1 penetration efficacy test
The penetration rate of the cosmetic added with 0.5 percent of nano gold oil peptide is tested by Raman spectrum by taking nicotinamide as a marker.
Table 1 penetration efficacy test results
| Content (%) | Permeability (%) | |
| 2 |
2 | 13.4 |
| Example 1 | 0.8 | 28.6 |
| Example 2 | 1.5 | 33.5 |
| Example 3 | 1.0 | 27.5 |
| Comparative example 1 | 0.7 | 15.2 |
| Comparative example 2 | 0.8 | 17.9 |
| Comparative example 3 | 0.5 | 10.6 |
As can be seen from Table 1, the permeability of the nano gold oil peptide prepared in the embodiments 1-3 of the invention can reach as high as 33.5%.
Test example 2 moisturizing efficacy test
The moisturizing efficacy of the samples was evaluated by instrumental testing comparing the change in moisture of the skin before and after use of the samples.
The test method comprises the following steps:
and (3) moisturizing: for blank control, the forearm was selected as the test site and the moisture content of the skin was measured before and after 1 hour of sample application.
Blank control: no treatment and no smearing of the sample
Table 2 moisturizing efficacy test results
As shown in table 2, the nano gold oil peptides prepared in examples 1 to 3 of the present invention have excellent moisturizing effect, and have significant technical effect compared to those of comparative examples 1 to 3.
Test example 3 antioxidant efficacy test
By testing the nano-gold oil peptide on DPPH free radical and ABTS + Scavenging ability of free radicals, testing the correspondingThe antioxidant efficacy, the test results are shown in table 3.
Method for testing DPPH radicals:
positive controls consisted of boscalid (50 ppm), tretinoin (50 ppm), acetyl hexapeptide (50 ppm), and complex peptides (acetyl hexapeptide-8, tripeptide-1 and pentapeptide-3, 50 ppm) added to a 96-well plate at 2 x 10 -4 Adding 100 μ L of DPPH ethanol solution at mol/L, adding 100 μ L of solution to be tested, shaking, standing at room temperature in dark for 30min, and measuring absorbance A at 517nm t Measuring background absorbance value A by using ethanol instead of DPPH solution r Measuring absorbance A of the reference solution by using ultrapure water instead of the sample solution 0 The same sample was tested 3 times and the clearance was calculated according to the following formula.
DPPH radical clearance (%) = {1- (a) t -A r )/A 0 }*100
ABTS + The free radical test method is as follows:
using vitamin C as positive control, 5ml of 7mmol/LABTS and 88. Mu.L of 140mmol/LK were added 2 S 2 O 8 Mixing, standing overnight at room temperature in dark (12 h) to form ABTS + Free radical stock solution, and diluted to ABTS with Absolute ethanol at 0.7 Absorbance before use + Working liquid (734 nm wavelength). When the assay was performed, 100. Mu. LABTS was added to a 96-well plate + Adding 100 μ L of the working solution, shaking, and measuring absorbance A at 734nm after 10min t By replacing ABTS with ethanol + Background absorbance value A measured by working solution r Measuring absorbance A of the reference solution by using ultrapure water instead of the sample solution 0 The same sample was tested 3 times, and the clearance was calculated according to the following equation.
ABTS + Radical clearance (%) = {1- (a) t -A r )/A 0 }*100
TABLE 3 scavenging ability (%)
| Sample (I) | DPPH clearance rate | ABTS + Clearance rate |
| Vitreous chromogen | 66.1 | 62.7 |
| Vitamin A alcohol | 75.6 | 74.4 |
| Acetyl hexapeptide | 87.5 | 86.4 |
| Complex peptides | 91.4 | 92.7 |
| Example 1 | 93.4 | 93.9 |
| Example 2 | 94.3 | 95.1 |
| Example 3 | 93.9 | 94.2 |
| Comparative example 1 | 74.3 | 70.8 |
| Comparative example 2 | 85.5 | 84.2 |
| Comparative example 3 | 69.3 | 70.3 |
As can be seen from Table 3, compared with the antioxidant products with the same particle size in the prior art, the nano gold oil peptides prepared in the embodiments 1 to 3 of the present invention have excellent antioxidant performance. In contrast, in comparative examples 1 to 3, the oxidation resistance was reduced to various degrees depending on the A phase, B phase or shear emulsification method.
Test example 4 test for adipocyte Activity
The proliferation efficacy of the nano-gold oil peptide on adipocytes was tested by testing different concentrations of the nano-gold oil peptide of example 3 on adipocyte precursor cells (PGM-2) and adipocytes (3T 3-L1), and the activity on adipocytes was tested, and the test results are shown in Table 4.
The test method comprises the following steps: adipose precursor cells (PGM-2) and adipose cells (3T 3-L1) are used as research objects, the experimental group is nano gold oil peptide containing 0.1%, 0.2%, 0.5%, 1.0%, 1.5%, 2.0%, and the experimental group and the blank control group are respectively cultured by high-sugar DMEM medium containing 0.1% volume fraction of fetal calf serum.
The main observation indexes are as follows: the growth, proliferation and differentiation of 3T3-L1 preadipocytes in each group were observed under an inverted microscope and recorded by photography. And when the bottom of the bottle is full of cells and meets the monolayer confluence, measuring the proliferation rate of the fat content in the 3T3-L1 preadipocytes by using an oil red O staining extraction method.
As a result: (1) observing the 3T3-L1 preadipocytes before differentiation in a fusiform manner under an inverted microscope, wherein lipid drops do not exist in cytoplasm; the differentiated 3T3-L1 preadipocytes are circular, a large number of lipid droplets are cut in cytoplasm, and the lipid droplets gather around the nucleus and are dyed to be orange-red by oil red O to form a ring-like shape; (2) the results of the tetramethylazoazolate colorimetric method and the oil red O staining extraction method show that the differentiation rate of 3T3-L1 preadipocytes and the proliferation rate of intracellular fat content in different concentrations are higher than those of a blank control group (P < 0.05), and the proliferation and differentiation effects on the 3T3-L1 preadipocytes are most obvious (P < 0.05).
TABLE 4 proliferation assay data of Nanogold oil peptides on adipocyte precursor cells (PGM-2) and adipocytes (3T 3-L1)
As can be seen from table 4, compared with the control example, the nano gold oil peptide prepared in example 3 of the present invention has an obvious effect of promoting proliferation and differentiation of 3T3-L1 preadipocytes, so that the nano gold oil peptide has an excellent effect of plumping adipocytes, and has a good effect of plumping fat on relaxed, face-broken and deep-wrinkled skin. However, the promoting effect is not proportional to the concentration, but is not increased after a certain dosage is reached, wherein the oil peptide with the concentration of 1.0% promotes the differentiation rate of the fat precursor cells (PGM-2) and the proliferation of the fat cells (3T 3-L1) to the maximum.
In conclusion, the nano gold oil peptide can permeate to the dermis, has the effects of high-efficiency permeation, water solubility, good compatibility, nutrition supplement, moisture preservation promotion, barrier repair, oxidation resistance, free radical resistance and the like, and can play the effects of tightening and wrinkle resistance in all directions.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. A preparation method of nano gold oil peptide capable of permeating to a dermis layer is characterized in that raw materials of the nano gold oil peptide are compounded and treated by adopting a high-pressure microjet homogenization method to obtain the nano gold oil peptide;
wherein the nano gold oil peptide comprises the following raw materials in parts by weight: 30-50 parts of adductor seed oil, 30-50 parts of synthetic esters or alkanes, 0.1-1.0 part of small molecular peptide, 0.1-0.5 part of dimethyl methoxy chromanol, 0.04-0.06 part of ceramide NP, 1-5 parts of humectant, 10-20 parts of polyglycerol emulsifier, 1.9-2.1 parts of additive and 2-5 parts of water.
2. The method of claim 1, wherein the synthetic esters include any one or both of caprylic/capric triglyceride and cetyl ethylhexanoate;
the alkane comprises any one or two of isomeric hexadecane and C9-12 alkane;
the small molecule peptide comprises the following raw materials in parts by weight: palmitoyl tripeptide-1.004-0.006 part, palmitoyl pentapeptide-4.004-0.006 part and acetyl octapeptide-3.1-1.0 part.
3. The preparation method according to claim 1, wherein the additive comprises the following raw materials in parts by weight: 0.04-0.06 part of phytosterol oleate, 2.0-4.0 parts of hydrogenated polyisobutene, 0.1-0.5 part of isosorbide dimethyl ether, 0.1-1.0 part of tocopherol and 0.08-0.2 part of rhizoma anemarrhenae root extract;
the content of the timosaponin in the rhizoma anemarrhenae root extract is 10 mu M-20 mu M;
the polyglycerol emulsifier comprises one or more of polyglycerol-2 caprate, polyglycerol-6 caprylate, polyglycerol-2 sesquioleate, polyglycerol-10 dioleate and polyglycerol-6 didecanate;
the humectant comprises any one or more of glycerin, butylene glycol and 1, 2-hexanediol.
4. The method according to claim 1, further comprising 1 to 2 parts of sesame seed extract.
5. The preparation method according to claim 1, wherein the high-pressure micro-jet homogenization is carried out under a pressure of 20-300MPa, at a temperature of 25-35 ℃ and for a time of 4-6min.
6. The preparation method according to claim 2, characterized by comprising the following steps:
s1, mixing and stirring water, a humectant, palmitoyl tripeptide-1, palmitoyl pentapeptide-4 and acetyl octapeptide-3 until the mixture is dissolved and transparent to obtain a phase A mixed solution;
s2, mixing and stirring the rest components until the rest components are dissolved to obtain a B-phase mixed solution;
s3, adding the A-phase mixed solution into the B-phase mixed solution, and continuously stirring to obtain an emulsion;
s4, carrying out high-pressure microjet homogenization treatment on the emulsion obtained in the step S3 to obtain nano gold oil peptide;
wherein, the steps S1 and S2 have no requirement of the sequence.
7. The preparation method according to claim 6, wherein in step S2, the remaining components are mixed and heated to 75-85 ℃, and the mixture is stirred and dissolved to obtain a B-phase mixed solution.
8. The method according to claim 6, wherein in step S3, the phase A mixture is added to the phase B mixture after the phase B mixture is cooled to room temperature.
9. A nano gold oil peptide capable of permeating to a dermis layer, which is prepared according to the preparation method of any one of claims 1-8;
preferably, the nano golden oil peptide is nano emulsion with the particle size of 100-1000 nm.
10. Use of the nano gold oil peptide of claim 9 in the preparation of a skin care product, wherein the nano gold oil peptide can permeate into a dermis layer.
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