CN115279498A - Biochemical reaction device and application thereof - Google Patents
Biochemical reaction device and application thereof Download PDFInfo
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- CN115279498A CN115279498A CN202080098299.2A CN202080098299A CN115279498A CN 115279498 A CN115279498 A CN 115279498A CN 202080098299 A CN202080098299 A CN 202080098299A CN 115279498 A CN115279498 A CN 115279498A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
Abstract
A biochemical reaction apparatus (100) includes a reaction container (10) and a partition (30). The reaction vessel (10) comprises a first end wall (11) and a second end wall (13) which are oppositely arranged, and a side wall (15) connecting the first end wall (11) and the second end wall (13), wherein the first end wall (11), the second end wall (13) and the side wall (15) enclose a sealed cavity (16). The partition part (30) is arranged on the side wall (15) and divides the sealed cavity (16) into a first reaction cavity (161) and a second reaction cavity (163) which are isolated from each other, the first reaction cavity (161) is used for containing a first reaction reagent, and the second reaction cavity (163) is used for containing a second reaction reagent. Wherein the partition (30) can be opened to mix the second reactive agent and the first reactive agent. A CRISPR technology based detection system comprising the biochemical reaction device (100) is also provided.
Description
The invention relates to the field of biochemical reaction devices, in particular to a biochemical reaction device capable of sealing a reaction device and preventing products from polluting and application thereof.
The application of the CRISPR system in the academic world is very wide, the CRISPR system mainly focuses on the gene editing function, and in recent years, with the continuous development of the technology and the development of a novel CRISPR system, application products utilizing the technology emerge in succession. At present, application products developed by the CRISPR system using Cas9, cas12, cas13, or Cas14 are mainly used. Among them, CRISPR systems using Cas12, cas13, or Cas14 are useful for pathogen detection. During detection, cas12, cas13 or Cas14 binds to a specific gRNA to form a complex, and under the guidance of the gRNA, a target nucleic acid sequence can be specifically recognized (Cas 12 mainly recognizes double-stranded DNA, cas13 mainly recognizes RNA, and Cas14 mainly recognizes single-stranded DNA), and is cleaved, and the recognition of the target nucleic acid sequence also triggers the nonspecific cleavage of surrounding single-stranded DNA or RNA.
When the existing detection system based on the CRISPR technology carries out pathogen detection, firstly, a target DNA fragment to be detected needs to be amplified and enriched, and then an amplification product is added into the CRISPR detection system for signal detection; wherein the amplification reaction and the CRISPR detection reaction are performed in separate test tubes (reaction systems), respectively. When a single separated test tube is used for carrying out multi-step reaction for detection, product molecules are exposed out of the test tube in the process of transferring a reaction product from one test tube to another test tube, so that the product molecules are easy to pollute the working environment, and the subsequent sample detection has false positive reaction.
Disclosure of Invention
In view of the above, it is necessary to provide a biochemical reaction apparatus and its application capable of preventing the product from being polluted.
An embodiment of the present invention provides a biochemical reaction apparatus including a reaction container and a partition. The reaction vessel comprises a first end wall, a second end wall and a side wall, wherein the first end wall and the second end wall are arranged oppositely, the side wall is connected with the first end wall and the second end wall, and the first end wall, the second end wall and the side wall enclose a sealed cavity. The partition portion is arranged on the side wall and divides the sealed cavity into a first reaction cavity and a second reaction cavity which are mutually isolated, the first reaction cavity is used for containing a first reaction reagent, and the second reaction cavity is used for containing a second reaction reagent. Wherein the partition is openable to mix the second reactive agent and the first reactive agent.
In one embodiment, the biochemical reaction apparatus further includes a driving member movably disposed on the reaction container and including a first end and a second end disposed opposite to each other, the first end is connected to the partition, the second end is exposed outside the reaction container, and the driving member is configured to drive the partition to open.
In one embodiment, the sidewall has a first through hole, the second end passes through the first through hole and is exposed outside the reaction container, and the driving member is configured to pull the partition portion to move toward the sidewall to tear the partition portion.
In one embodiment, the first end wall or the second end wall is provided with a second through hole, the second end passes through the second through hole and is exposed outside the reaction container, one end of the partition portion is fixedly arranged on the side wall, the other end of the partition portion is movably arranged on the side wall, and the driving member is used for pushing the partition portion to move towards the corresponding second end wall or the first end wall so as to open the partition portion.
In one embodiment, the partition portion includes a protruding portion protruding toward the first end wall or the second end wall, the protruding portion is provided with an accommodating cavity and a through hole which are communicated with each other, the accommodating cavity and the through hole are communicated with the first reaction cavity and the second reaction cavity together, and the through hole enables the first reaction reagent or the second reaction reagent accommodated in the accommodating cavity not to flow out of the through hole when no external pressure is applied.
In one embodiment, the biochemical reaction apparatus further includes a driving member movably disposed on the reaction container and capable of pressing the first reaction reagent or the second reaction reagent accommodated in the accommodating cavity to flow out of the through hole.
In one embodiment, the driving member includes a driving portion and a partition plate, the driving portion is movably disposed on the first end wall or the second end wall, and the partition plate is slidably connected to the side wall and divides the first reaction chamber or the second reaction chamber into a first portion and a second portion that are isolated from each other.
In one embodiment, the reaction vessel comprises a resilient portion capable of being deformed to force the first or second reactive agent contained in the holding chamber to flow out of the through hole.
In one embodiment, the reaction container includes a first reaction portion and a second reaction portion, the first reaction portion defines a first cavity, the second reaction portion defines a second cavity, and the second reaction portion is detachably mounted on the first reaction portion such that the second cavity and the first cavity form a sealed cavity together.
An embodiment of the present invention further provides a detection system based on CRISPR technology, including any one of the above biochemical reaction apparatuses, a first reaction reagent, and a second reaction reagent. The first reaction reagent is contained in the first reaction cavity and is used for enabling a sample to be detected to generate amplification enrichment reaction. The second reaction reagent is contained in the second reaction cavity and is used for enabling the amplification enriched product to perform CRISPR detection reaction.
When the biochemical reaction device provided by the invention is used, the cover opening operation is not needed, and the whole biochemical reaction process is carried out in the sealed cavity of the reaction container, so that the pollution of reaction products to the external working environment is avoided, and the false positive reaction is prevented.
FIG. 1 is a schematic structural diagram of a biochemical reaction apparatus according to a first embodiment of the present invention.
FIG. 2 is a schematic structural diagram of a biochemical reaction apparatus according to a second embodiment of the present invention.
FIG. 3 is a schematic structural diagram of a biochemical reaction apparatus according to a third embodiment of the present invention.
FIG. 4 is a schematic structural diagram of a biochemical reaction apparatus according to a fourth embodiment of the present invention.
FIG. 5 is a schematic structural diagram of a biochemical reaction apparatus according to a fifth embodiment of the present invention.
Description of the main elements
Sealed chamber 16
First through hole 151
Driving member 50
Projecting part 32
Through hole 323
The following detailed description will further illustrate the invention in conjunction with the above-described figures.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention and the scope of the present invention is therefore not limited to the specific embodiments disclosed below.
Referring to fig. 1, fig. 1 is a schematic structural diagram of a biochemical reaction apparatus 100 according to a first embodiment of the invention. The biochemical reaction apparatus 100 includes a reaction container 10 and a partition 30. The reaction vessel 10 includes a first end wall 11 and a second end wall 13 disposed opposite to each other, and a side wall 15 connecting the first end wall 11 and the second end wall 13. The first end wall 11, the second end wall 13 and the side wall 15 enclose a sealed chamber 16. The sealed chamber 16 is not in communication with the external working environment. The partition 30 is disposed on the sidewall 15 and divides the sealed chamber 16 into a first reaction chamber 161 and a second reaction chamber 163 which are isolated from each other. The first reaction chamber 161 is used for containing a first reaction reagent, and the first reaction reagent is a reactant capable of generating a first biochemical reaction; the second reaction chamber 163 is used for containing a second reaction reagent, which is a reactant capable of performing a second biochemical reaction with a reaction product obtained from the first biochemical reaction. The partition 30 can be opened to communicate the first reaction chamber 161 and the second reaction chamber 163, so that the reaction product in the first reaction chamber 161 and the reactant in the second reaction chamber 163 are mixed to generate a second biochemical reaction. The whole biochemical reaction process is carried out in the sealed cavity 16 of the reaction vessel 10, thereby avoiding the pollution of the reaction product to the external working environment and preventing false positive reaction.
The periphery of the partition 30 is fixedly attached to the side wall 15. The sidewall 15 is formed with a first through hole 151, and the first through hole 151 is disposed adjacent to one side of the partition 30. The biochemical reaction apparatus 100 further includes a driving member 50 movably disposed on the sidewall 15, wherein the driving member 50 is used for driving the partition 30 to open. The driving member 50 includes a first end 51 and a second end 53 disposed opposite to each other. The first end 51 is connected to a side of the partition 30 away from the first through hole 151, and the second end 53 is exposed out of the sidewall 15 through the first through hole 151. When the driving member 50 is pulled, the partition 30 moves toward the side wall 15 along with the driving member 50 to tear the partition 30, so that the partition 30 is opened, so that the first reactive agent of the first reaction chamber 161 and the second reactive agent of the second reaction chamber 163 can be mixed. In this embodiment, the partition 30 is a film, and the driving member 50 is a pull rod.
Optionally, a sealing member (not shown) is disposed between the first through hole 151 and the driving member 50 to seal the sealing cavity 16.
The reaction vessel 10 includes a first reaction part 12 and a second reaction part 14. The first reaction part 12 comprises a bottom wall and a side wall surrounding the bottom wall, wherein the bottom wall and the side wall surround to form a first cavity. The second reaction part 14 includes a bottom wall and a side wall surrounding the bottom wall, wherein the bottom wall and the side wall surround to form a second cavity. The side wall of the second reaction portion 14 is detachably mounted on the side wall of the first reaction portion 12, so that the first cavity and the second cavity together form the sealed cavity 16. The side wall of the first reaction part 12 and the side wall of the second reaction part 14 together constitute the side wall 15 of the reaction vessel 10, the bottom wall of the first reaction part 12 serves as the first end wall 11 of the reaction vessel 10, and the bottom wall of the second reaction part 14 serves as the second end wall 13 of the reaction vessel. In this embodiment, the first reaction part 12 is a test tube, and the second reaction part 14 is a cap.
In the present embodiment, the side wall of the second reaction part 14 is screwed to the side wall of the first reaction part 12. In other embodiments, the second reaction portion 14 may be detachably connected to the first reaction portion 12 by other connecting means, such as an interference fit.
Optionally, a sealing member (not shown) may be further disposed between the sidewall of the second reaction portion 14 and the sidewall of the first reaction portion 12, for sealing the sealing cavity 16.
In the present embodiment, the periphery of the partition 30 is fixedly connected to the sidewall of the second reaction portion 14; the second reaction chamber 163 is located in the second cavity of the second reaction part 14, and the first reaction chamber 161 is located in the first cavity of the first reaction part 12 and a part of the second cavity of the second reaction part 14; the driving member 50 and the first through hole 151 are located at a side of the partition 30 away from the first reaction part 12. When the driving member 50 is pulled, the second reactive agent in the second reaction chamber 163 flows into the first reaction chamber 161 to be mixed with the first reactive agent. It is understood that in other embodiments, the first reaction chamber 161 can be used for containing a second reaction reagent, the second reaction chamber 163 can be used for containing a first reaction reagent, and when the driving member 50 is pulled, the first reaction reagent can flow into the second reaction reagent for mixing.
The biochemical reaction apparatus 100 is suitable for a biochemical reaction system having two or more reaction steps, and in which a reaction product obtained in one reaction step is mixed with a reactant in a subsequent step to perform a reaction; the device can also be applied to biochemical reaction systems which need to be opened and additionally added with reagents. The biochemical reaction apparatus 100 can be applied to an RNA reverse transcription system or a detection system based on CRISPR technology. In the present embodiment, the biochemical reaction apparatus 100 is exemplified by being applied to a detection system based on CRISPR technology.
The detection system based on the CRISPR technique comprises the biochemical reaction device 100, a first reaction reagent and a second reaction reagent. The first reaction reagent is pre-disposed in the first reaction chamber 161, and is used for performing amplification and enrichment reaction on the DNA fragment to be detected. The first reaction reagent can adopt any reagent which can amplify and enrich the DNA fragments. The second reaction reagent is pre-disposed in the second reaction chamber 163 for performing a CRISPR detection reaction on the amplified enriched product. The second reaction reagent can adopt any reagent required for carrying out CRISPR detection reaction.
After the amplification and enrichment reaction between the first reaction reagent in the first reaction chamber 161 and the DNA to be detected occurs, the driving member 50 pulls the separating portion 30 to move toward the side wall 15, so that the separating portion 30 is torn in a direction perpendicular to the side wall 15, and the second reaction reagent in the second reaction chamber 163 flows into the first reaction chamber 161 to be mixed with the amplification and enrichment product. In this embodiment, the second reaction reagent is added to the amplification-enriched product, so that the amplification-enrichment cycle time can be greatly shortened, and the overall detection time can be shortened.
Referring to fig. 2, fig. 2 is a schematic structural diagram of a biochemical reaction apparatus 100 according to a second embodiment of the invention. The biochemical reaction apparatus 100 of the present embodiment is different from the biochemical reaction apparatus 100 of the first embodiment in that the driving member 50 is movably disposed on the second end wall 13, and one end of the partition 30 is movably disposed on the side wall 15, so that the driving member 50 pushes the partition 30 to open toward the first end wall 11, so as to change the opening manner of the partition 30. It will be appreciated that in other embodiments, the driving member 50 may also be movably disposed on the first end wall 11, and the driving member 50 can push one end of the partition 30 to be opened toward the second end wall 13.
In this embodiment, the second end wall 13 is opened with a second through hole (not shown), and the second end 53 of the driving member 50 passes through the second through hole and extends out of the reaction vessel 10. In this embodiment, the driving member 50 is a key assembly. One end of the partition 30 is fixedly connected to the side wall 15, and the other end of the partition 30 is movably connected to the side wall 15, so that when the driving member 50 pushes the partition 30 to move toward the first end wall 11, one end of the partition 30 opens toward the first end wall 11, and thus the reaction product in the first reaction chamber 161 and the reaction product in the second reaction chamber 163 are mixed to generate a second biochemical reaction.
When the biochemical reaction apparatus 100 of the present embodiment is applied to a detection system based on CRISPR technology, after the amplification and enrichment reaction between the first reaction reagent in the first reaction chamber 161 and the DNA to be detected occurs, the driving member 50 pushes the separating part 30 to move towards the first end wall 11, so that the end of the separating part 30 movably connected with the side wall 15 is opened towards the first end wall 11, and the second reaction reagent in the second reaction chamber 163 flows into the first reaction chamber 161 to be mixed with the amplification and enrichment product.
Referring to fig. 3, fig. 3 is a schematic structural diagram of a biochemical reaction apparatus 100 according to a third embodiment of the invention. The biochemical reaction apparatus 100 of the present embodiment is different from the biochemical reaction apparatus 100 of the second embodiment in that the partition 30 is not opened by the driving of the driving member 50 but opened by the pressure variation of one side of the partition 30.
Specifically, the reaction vessel 10 includes an elastic portion that can be deformed to increase the pressure on the side of the partition 30, thereby forcing the end of the partition 30 that is movably connected to the side wall 15 to open toward the first end wall 11. The elastic portion may be located at least one of the side wall 15, the first end wall 11, and the second end wall 13 of the reaction vessel 10. In the present embodiment, the second reaction part 14 is made of an elastic material, and the entire second reaction part 14 is an elastic portion of the reaction vessel 10. When the second reaction part 14 is compressively deformed, the pressure of the partition 30 at the side of the second reaction chamber 163 is increased, thereby forcing the end of the partition 30 movably connected with the sidewall 15 to be opened toward the first end wall 11. It is understood that, in other embodiments, the first reaction part 12 may be made of an elastic material, the first reaction part 12 is integrally formed as an elastic part of the reaction vessel 10, and when the first reaction part 12 is compressed and deformed, the pressure of the partition 30 on the side of the first reaction chamber 161 is increased, so that the end of the partition 30 movably connected with the side wall 15 is pressed to be opened toward the second end wall 13.
When the biochemical reaction apparatus 100 of the present embodiment is applied to the detection system based on CRISPR technology, after the first reaction reagent and the DNA to be detected in the first reaction chamber 161 undergo amplification and enrichment reaction, the first reaction portion 12 is compressed and deformed, so as to increase the pressure of the separation portion 30 at one side of the first reaction chamber 161, thereby pressing the end of the separation portion 30 movably connected to the side wall 15 to open toward the second end wall 13, so that the second reaction reagent in the second reaction chamber 163 flows into the first reaction chamber 161 and mixes with the amplification and enrichment product.
Referring to fig. 4, fig. 4 is a schematic structural diagram of a biochemical reaction apparatus 100 according to a fourth embodiment of the invention. The biochemical reaction apparatus 100 according to the present embodiment is different from the biochemical reaction apparatus 100 according to the second embodiment in that the partition 30 has a different structure and the driving member 50 and the partition 30 have a different connection relationship.
The partition 30 includes a protrusion 32 formed to protrude toward the first end wall 11. The protruding portion 32 is sequentially provided with an accommodating cavity 321 and a through hole 323 along a direction perpendicular to the first end wall 11, and an outlet (not shown) is provided at an end of the through hole 323 departing from the accommodating cavity 321. The through hole 323 is communicated with the accommodating cavity 321, and the accommodating cavity 321 and the through hole 323 are communicated with the second reaction cavity 163 and the first reaction cavity 161 together. The through hole 323 can prevent the second reactive agent contained in the containing cavity 321 from flowing out of the through hole 323 when the second reactive agent is not subjected to the external pressure applied by the driving member 50. In the present invention, when the second reactive agent in the accommodating chamber 321 cannot flow out of the through hole 323, the partition 30 is in an unopened state; when the second reactive agent in the receiving chamber 321 can flow out from the through hole 323, the partition 30 is in an open state.
In this embodiment, the accommodating cavity 321 is substantially in the shape of a truncated inverted cone, the through hole 323 is substantially in the shape of a cylinder, and the diameter of the through hole 323 is smaller than or equal to the minimum diameter of the accommodating cavity 321. In this embodiment, when the second reactive agent in the accommodating chamber 321 is only acted by the pressure of the air in the first reaction chamber 161 and the second reaction chamber 163, and is not acted by the external pressure, it does not flow out of the through hole 323 under the action of the air pressure. That is, when the second reactive agent contained in the containing chamber 321 is not subjected to the external pressure applied by the driving member 50, the pressure applied by the air in the first reaction chamber 161 to the second reactive agent should be greater than the pressure applied by the air in the second reaction chamber 163 to the second reactive agent, so that the second reactive agent does not flow out of the through hole 323.
The drive member 50 includes a drive portion 52 and a spacer 54 connected to each other. The driving part 52 is movably installed in the second through hole and partially extends out of the reaction vessel 10. The partition plate 54 is slidably coupled to the sidewall 15 and partitions the second reaction chamber 163 into a first portion and a second portion isolated from each other. The second part is used for containing the second reaction reagent. When the driving part 52 pushes the partition plate 54 to slide along the side wall 15, the volume of the first portion in the second reaction chamber 163 increases, and the volume of the second portion in the second reaction chamber 163 decreases, so that the second reactive agent flows out from the through hole 323 to the first reaction chamber 161 under the external pressure to be mixed with the first reactive agent.
It is understood that, in other embodiments, the protrusion 32 may also be formed to protrude toward the second end wall 13, and the driving portion 52 is movably disposed on the first end wall 11 and can push the partition plate 54 to press the first reaction reagent in the first reaction chamber 161 to flow out through the through hole 323 to the second reaction chamber 163 to be mixed with the second reaction reagent.
When the biochemical reaction apparatus 100 of the present embodiment is applied to the detection system based on CRISPR technology, after the amplification and enrichment reaction between the first reaction reagent in the first reaction chamber 161 and the DNA to be detected occurs, the driving portion 52 pushes the partition plate 54 to press the second reaction reagent in the second reaction chamber 163 to flow out through the through hole 323 to the first reaction chamber 161 to be mixed with the amplification and enrichment product.
Referring to fig. 5, fig. 5 is a schematic structural diagram of a biochemical reaction apparatus 100 according to a fifth embodiment of the invention. The biochemical reaction apparatus 100 of the present embodiment is different from the biochemical reaction apparatus 100 provided in the fourth embodiment in that the partition 30 is not opened by the driving of the driving member 50, but is opened by the pressure variation of one side of the partition 30.
Specifically, the reaction vessel 10 includes an elastic portion that is deformable to increase a pressure of a side of the second reactive agent received in the receiving chamber 321, thereby forcing the second reactive agent to flow out of the through hole 323 into the first reaction chamber 161 to be mixed with the first reactive agent. In the present embodiment, the second reaction part 14 is made of an elastic material, and when the second reaction part 14 is compressively deformed, the pressure of the second reaction agent side received in the receiving chamber 321 is increased, thereby pressing the second reaction agent to flow out of the through hole 323 to open the partition 30.
It is understood that, in other embodiments, the protrusion 32 may be formed to protrude toward the second end wall 13, the first reaction part 12 may be made of an elastic material, and when the first reaction part 12 is compressively deformed, the pressure of the side of the first reaction reagent accommodated in the accommodating chamber 321 is increased, thereby forcing the first reaction reagent to flow out of the through hole 323 to open the partition 30. In the present embodiment, the first reaction part 12 and the second reaction part 14 are both test tubes.
When the biochemical reaction apparatus 100 of the present embodiment is applied to a detection system based on CRISPR technology, after the first reaction reagent in the first reaction chamber 161 and the DNA to be detected undergo amplification and enrichment reactions, the second reaction portion 14 is compressed and deformed to press the second reaction reagent in the second reaction chamber 163 to flow out through the through hole 323 to the first reaction chamber 161 to be mixed with the amplification and enrichment product.
The foregoing embodiments are merely illustrative of the principles of this invention and are not to be construed as limiting, various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
- A biochemical reaction apparatus comprising:the reaction container comprises a first end wall, a second end wall and a side wall, wherein the first end wall and the second end wall are arranged oppositely, the side wall is connected with the first end wall and the second end wall, and the first end wall, the second end wall and the side wall enclose a sealed cavity; andthe partition part is arranged on the side wall and divides the sealed cavity into a first reaction cavity and a second reaction cavity which are mutually isolated, the first reaction cavity is used for containing a first reaction reagent, and the second reaction cavity is used for containing a second reaction reagent;wherein the partition is openable to mix the second reactive agent and the first reactive agent.
- The biochemical reaction device according to claim 1, further comprising a driving member movably disposed on the reaction container and including a first end and a second end disposed opposite to each other, wherein the first end is connected to the partition, the second end is exposed outside the reaction container, and the driving member is configured to drive the partition to open.
- The biochemical reaction device according to claim 2, wherein the sidewall has a first through hole, the second end is exposed outside the reaction container through the first through hole, and the driving member is configured to pull the partition portion toward the sidewall to tear the partition portion.
- The biochemical reaction device according to claim 2, wherein the first end wall or the second end wall has a second through hole, the second end passes through the second through hole and is exposed outside the reaction container, one end of the partition is fixedly disposed on the side wall, the other end of the partition is movably disposed on the side wall, and the driving member is configured to push the partition to move toward the corresponding second end wall or the first end wall to open the partition.
- The biochemical reaction device according to claim 1, wherein the partition portion includes a protrusion portion protruding toward the first end wall or the second end wall, the protrusion portion defines a receiving chamber and a through hole communicating with each other, the receiving chamber and the through hole communicate with the first reaction chamber and the second reaction chamber, and the through hole enables the first reaction reagent or the second reaction reagent received in the receiving chamber not to flow out of the through hole when no external pressure is applied.
- The biochemical reaction device according to claim 5, further comprising a driving member movably disposed on the reaction container and capable of pressing the first reaction reagent or the second reaction reagent contained in the containing cavity to flow out of the through hole.
- The biochemical reaction device according to claim 6, wherein the driving member comprises a driving portion movably disposed on the first end wall or the second end wall, and a partition plate slidably connected to the side wall and dividing the first reaction chamber or the second reaction chamber into a first portion and a second portion isolated from each other.
- The biochemical reaction device according to claim 5, wherein the reaction vessel includes an elastic portion that is deformable to press the first reactive agent or the second reactive agent contained in the containing chamber to flow out of the through hole.
- The biochemical reaction device according to claim 1, wherein the reaction container comprises a first reaction portion and a second reaction portion, the first reaction portion defines a first cavity, the second reaction portion defines a second cavity, and the second reaction portion is detachably mounted on the first reaction portion such that the second cavity and the first cavity together form a sealed cavity.
- A CRISPR technology based detection system comprising:the biochemical reaction device according to any one of claims 1 to 9;the first reaction reagent is accommodated in the first reaction cavity and is used for enabling a sample to be detected to generate amplification enrichment reaction; andand the second reaction reagent is contained in the second reaction cavity and is used for enabling the amplification-enriched product to perform CRISPR detection reaction.
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