CN205687906U - A kind of PCR quick detection kit - Google Patents
A kind of PCR quick detection kit Download PDFInfo
- Publication number
- CN205687906U CN205687906U CN201620606290.9U CN201620606290U CN205687906U CN 205687906 U CN205687906 U CN 205687906U CN 201620606290 U CN201620606290 U CN 201620606290U CN 205687906 U CN205687906 U CN 205687906U
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- CN
- China
- Prior art keywords
- container
- pcr
- supporting plate
- detection kit
- centrifuge tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model discloses a kind of PCR quick detection kit, the operating procedure being a simplified reagent preparation will be solved the technical problem that, save the time, decrease the probability of operational error.The inner chamber of this utility model box body is provided with horizontally disposed supporting plate, supporting plate is provided with several spaced centrifugal pores, it is placed with the centrifuge tube equipped with DNA extraction liquid in centrifugal pore, the mouth of pipe of centrifuge tube is provided with flexible connection and the container equipped with prefabricated PCR detectable, the lower surface of container is provided with container hole, and container is provided with extrusion mechanism.Compared with prior art, greatly simplify the operating procedure of reagent preparation, save the time, decrease the probability of operational error, greatly reduce the requirement to operator's technical merit simultaneously.The PCR being particularly well-suited to sample in chain of evidence extracts, it is possible to shorten test in laboratory time, it is often more important that can reduce the probability that sample lost efficacy, have good market application foreground.
Description
Technical field
This utility model relates to a kind of field of bioexperiment technology, particularly a kind of PCR quick detection kit.
Background technology
At present, in diagnostic test, test kit is requisite detection instrument.But, the test kit used at present leads to
Being often to be sub-packed in different reagent bottle by the agent formulations that detection is relevant, tester needs substep to specifications difference to be tried
Reagent in agent bottle takes out to concentrate and loads in PCR pipe, just can carry out PCR amplification, complex operation step, and detection ratio is relatively time-consuming, and
Operator is easily generated maloperation；Again, existing PCR detection kit the most only comprises the detectable needed for PCR reaction, no
Including the reagent of extraction template, need additionally to buy the DNA extraction kit of processing template, add testing cost；Meanwhile, existing
The hole having test kit to deposit Reagent Tube cannot regulate, it is impossible to meets the use demand of different size Reagent Tube.
Summary of the invention
The purpose of this utility model is to provide a kind of PCR quick detection kit, will solve the technical problem that and be a simplified
The operating procedure of reagent preparation, saves the time, decreases the probability of operational error.
For solving the problems referred to above, this utility model realizes by the following technical solutions: a kind of PCR quick detection kit,
Including box body, being provided with horizontally disposed supporting plate in the inner chamber of described box body, box body inner chamber top is located at by described supporting plate, on supporting plate
It is provided with several spaced centrifugal pores, in centrifugal pore, is placed with the centrifuge tube equipped with DNA extraction liquid, at centrifuge tube
The mouth of pipe is provided with flexible connection and the container equipped with prefabricated PCR detectable, and the lower surface of container is provided with container hole, described
Container is provided with and prefabricated PCR detectable is added the intraluminal extrusion mechanism of centrifuge tube.
Extrusion mechanism described in the utility model includes the piston being located in container, is provided with and pushes away on the upper surface of piston
Bar, push rod stretches out from the push-rod hole being located at container upper end face.
At least two thickness appearance less than container it is provided with in push-rod hole described in the utility model and between the bar wall of push rod
The connecting portion of wall thickness, constitutes dotted line hole between adjacent two connecting portions.
The middle part of supporting plate described in the utility model is provided with cavity.
Being provided with at least one fixed mechanism in centrifugal pore described in the utility model, this fixed mechanism includes setting centrifuge tube
The groove of on periphery wall one week annular, be located in supporting plate cavity be positioned at the push pedal at centrifugal pore, described push pedal is provided with and pushes away
Arm, is provided with back seat in the rear end of push arm, and push arm hole from the hole wall being located at centrifugal pore, the front end of described push arm is stretched into centrifugal
In pore, being provided with stretching spring between back seat and the cavity wall of supporting plate, one end of stretching spring is fixed on the internal chamber wall of supporting plate
On, the other end is fixed on back seat.
The cross sectional shape of groove described in the utility model is arc.
Fixed mechanism described in the utility model is provided with four.
Being provided with sealing ring between centrifuge tube described in the utility model and container, sealing ring is fixedly connected on the outer wall of container
On.
This utility model compared with prior art, arranges a flexible connection on the centrifuge tube equipped with DNA extraction liquid
Equipped with the container of prefabricated PCR detectable, greatly simplify the operating procedure of reagent preparation, save the time, decrease
The probability of operational error, greatly reduces the requirement to operator's technical merit simultaneously.Owing to increasing template extraction reagent, no
Reduced by only testing cost, provide maximum convenience for tester, owing to adding fixed mechanism in centrifugal pore, therefore can realize
One test kit is applicable to depositing of plurality of specifications test tube so that the use of test kit is more convenient, is particularly well-suited to chain of evidence
The PCR of middle sample extracts, it is possible to shorten test in laboratory time, it is often more important that can reduce the probability that sample lost efficacy,
There is good market application foreground.
Accompanying drawing explanation
Fig. 1 is the structural representation of this utility model test kit.
Fig. 2 is the attachment structure between this utility model centrifuge tube and container.
Fig. 3 is the attachment structure schematic diagram between this utility model push rod and push-rod hole.
Fig. 4 is the structural representation of this utility model fixed mechanism.
Fig. 5 is the attachment structure schematic diagram between this utility model centrifuge tube and centrifugal pore.
Detailed description of the invention
With embodiment, this utility model is described in further detail below in conjunction with the accompanying drawings.
As it is shown in figure 1, PCR quick detection kit of the present utility model includes box body 1, being provided with on box body 1 can be by
The lid 18 of the uncovered closing of box body 1, lid 18 realizes mutually fastening by the buckle structure of prior art with box body 1；
Being provided with horizontally disposed supporting plate 2 in the inner chamber of this box body 1, box body 1 inner chamber top is located at by described supporting plate 2, supporting plate 2
The luminal border of edge and box body 1 is combined closely, and is provided with several spaced centrifugal pores 3 on supporting plate 2, centrifugal
It is placed with the centrifuge tube 4 equipped with DNA extraction liquid 25 in pore 3, DNA extraction liquid 25 there was added magnesium ion, and then ensure that
The stability of pre-filled reagent inner reaction tube reagent storage, the general 0.2ml of DNA extraction liquid 25.
The middle part of described supporting plate 2 is provided with cavity, and supporting plate 2 is made up of upper cover 21 and base plate 22.
As in figure 2 it is shown, be provided with flexible connection and the appearance equipped with prefabricated PCR detectable 26 on the mouth of pipe of centrifuge tube 4
Device 5, in concrete connected mode is the mouth of pipe that the lower end of container 5 is inserted centrifuge tube 4, container 5 is cylindrical, its shape of cross section
And size is suitable with the orifice configuration of centrifuge tube 4 and size, the lower surface of container 5 is provided with container hole 6, container hole 6
On can be provided with extension tube 20, it is simple to adding in centrifuge tube 4 by prefabricated PCR detectable 26, container 5 is provided with to be examined prefabricated PCR
Test agent 26 adds the intraluminal extrusion mechanism of centrifuge tube 4, and extrusion mechanism includes the piston 7 being located in container 5, at piston 7
Upper surface is provided with push rod 8, and push rod 8 stretches out from the push-rod hole 9 being located at container 5 upper surface, and under resting state, piston 7 is positioned at
The cavity volume top of container 5, is provided with the pusher plate 19 facilitating finger push in the upper end of push rod 8, during use, promotes push rod 8, makes to live
Plug 7 moves bottom the cavity volume of container 5, is compressed by the air in container 5, drives the prefabricated PCR detectable being contained in container 5
26 get in centrifuge tube 4, mix with DNA extraction liquid 25, are extracted by container 5 after injection from centrifuge tube 4, so letter
Change operation, and the weight of test kit has also been minimized.
As it is shown on figure 3, be provided with at least two thickness container less than container 5 in push-rod hole 9 and between the bar wall of push rod 8
The connecting portion 10 of wall thickness, constitutes dotted line hole 11, constitutes between connecting portion 10 and dotted line hole 11 between adjacent two connecting portions 10
The strip breaking mechanism that can quickly fracture, so ensure that push rod 8 will not be because of collision by prefabricated PCR detection examination in transportation
Agent 26 is clamp-oned in centrifuge tube 4, and connecting portion 10 is preferably 4.
As shown in Figure 4 and Figure 5, being provided with at least one fixed mechanism in centrifugal pore 3, this fixed mechanism includes setting centrifuge tube
The groove 12 of on 4 periphery walls one week annular, in supporting plate 2 cavity, is positioned at centrifugal pore 3 and is provided with push pedal 13, and push pedal 13 sets
There is push arm 14, be provided with back seat 15, the front end of described push arm 14 pushing away from the hole wall being located at centrifugal pore 3 in the rear end of push arm 14
Arm aperture 27 stretches in centrifugal pore 3, is provided with stretching spring 16 between the cavity wall of back seat 15 and supporting plate 2, stretching spring 16
In the first draw-in groove 23 that one end is fixed on the internal chamber wall of supporting plate 2, in the second draw-in groove 24 that the other end is fixed on back seat 15, quiet
Only under state, centrifuge tube 4 is placed in centrifugal pore 3, when centrifuge tube 4 puts into centrifugal pore 3, and the outboard tube of centrifuge tube 4
Push arm 14 is pushed in push arm hole 27 by wall so that stretching spring 16 stretches；When groove 12 arrives push arm 14 location, push away
Push arm 14 is pushed in groove 12 because of the reset of stretching spring 16 by arm 14, it is achieved be fixed on by centrifuge tube 4 in centrifugal pore 3,
Therefore the centrifuge tube 4 that can be suitable for different-diameter uses.
The cross sectional shape of this utility model further groove 12 is arc；Fixed mechanism is preferably provided with four, is divided into two groups, often group
Two, often two fixed mechanism one_to_one corresponding respectively of group are arranged on the both sides that centrifugal pore 3 is relative.
In order to improve the sealing problem of centrifuge tube 4 and container 5 junction, between described centrifuge tube 4 and container 5, it is provided with sealing
Circle 17, sealing ring 17 is fixedly connected on the outer wall of container 5, when the prefabricated PCR detectable 26 in container 5 is clamp-oned completely.
This utility model has without early stage first by test agent mixing, but uses instead as directly by prefabricated PCR inspection
Test agent fill, in container, greatly simplify the operating procedure of reagent preparation, saves the time, decrease operational error
Probability, require to be greatly reduced to the technical merit of operator.Add the reagent of DNA extraction simultaneously, not only reduce
The expense of detection, also provides maximum facility for tester.
Claims (8)
1. a PCR quick detection kit, including box body (1), it is characterised in that: the inner chamber of described box body 1 is provided with level
The supporting plate (2) arranged, described supporting plate (2) is located at box body (1) inner chamber top, is provided with several spaced on supporting plate (2)
Centrifugal pore (3), is placed with the centrifuge tube (4) equipped with DNA extraction liquid (25), at the mouth of pipe of centrifuge tube (4) in centrifugal pore (3)
Being provided with flexible connection and the container (5) equipped with prefabricated PCR detectable (26), the lower surface of container (5) is provided with container
Hole (6), described container (5) is provided with and prefabricated PCR detectable (26) is added centrifuge tube (4) intraluminal extrusion mechanism.
A kind of PCR quick detection kit the most according to claim 1, it is characterised in that: described extrusion mechanism includes setting
Piston (7) in container (5), is provided with push rod (8) on the upper surface of piston (7), and push rod (8) is from being located at container (5) upper end
The push-rod hole (9) in face stretches out.
A kind of PCR quick detection kit the most according to claim 2, it is characterised in that: in described push-rod hole (9) with push away
At least two thickness connecting portion (10) less than the container wall thickness of container (5), adjacent two companies it are provided with between the bar wall of bar (8)
Connect and between portion (10), constitute dotted line hole (11).
A kind of PCR quick detection kit the most according to claim 1, it is characterised in that: the middle part of described supporting plate (2) sets
There is cavity.
A kind of PCR quick detection kit the most according to claim 4, it is characterised in that: set in described centrifugal pore (3)
Having at least one fixed mechanism, this fixed mechanism includes the groove (12) of one week that sets on centrifuge tube (4) periphery wall annular, is located at
The push pedal (13) being positioned at centrifugal pore (3) place in supporting plate (2) cavity, described push pedal (13) is provided with push arm (14), at push arm
(14) rear end is provided with back seat (15), push arm hole from the hole wall being located at centrifugal pore (3), the front end of described push arm (14)
(27) stretch in centrifugal pore (3), between the cavity wall of back seat (15) and supporting plate (2), be provided with stretching spring (16), stretch bullet
One end of spring (16) is fixed on the internal chamber wall of supporting plate (2), and the other end is fixed on back seat (15).
A kind of PCR quick detection kit the most according to claim 5, it is characterised in that: the cross section of described groove (12)
It is shaped as arc.
A kind of PCR quick detection kit the most according to claim 6, it is characterised in that: described fixed mechanism is provided with four
Individual.
8. according to a kind of PCR quick detection kit described in claim 1 or 7, it is characterised in that: described centrifuge tube (4) with
Being provided with sealing ring (17) between container (5), sealing ring (17) is fixedly connected on the outer wall of container (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620606290.9U CN205687906U (en) | 2016-06-20 | 2016-06-20 | A kind of PCR quick detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620606290.9U CN205687906U (en) | 2016-06-20 | 2016-06-20 | A kind of PCR quick detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205687906U true CN205687906U (en) | 2016-11-16 |
Family
ID=57415805
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620606290.9U Expired - Fee Related CN205687906U (en) | 2016-06-20 | 2016-06-20 | A kind of PCR quick detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205687906U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111077328A (en) * | 2019-12-31 | 2020-04-28 | 绿城农科检测技术有限公司 | Quick detection device capable of being simultaneously suitable for implementing test paper detection and reagent detection |
| CN115279498A (en) * | 2020-07-17 | 2022-11-01 | 深圳华大智造科技股份有限公司 | Biochemical reaction device and application thereof |
-
2016
- 2016-06-20 CN CN201620606290.9U patent/CN205687906U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111077328A (en) * | 2019-12-31 | 2020-04-28 | 绿城农科检测技术有限公司 | Quick detection device capable of being simultaneously suitable for implementing test paper detection and reagent detection |
| CN115279498A (en) * | 2020-07-17 | 2022-11-01 | 深圳华大智造科技股份有限公司 | Biochemical reaction device and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information |
Inventor after: Fu Wei Inventor after: Zhu Shuifang Inventor after: Li Xinshi Inventor after: Wei Shuang Inventor after: Ma Xinhua Inventor after: Wang Chenguang Inventor after: Zhu Pengyu Inventor after: Long Yang Inventor after: Yuan Junjie Inventor after: Lin Chungui Inventor before: Fu Wei Inventor before: Wei Shuang Inventor before: Ma Xinhua Inventor before: Wang Chenguang Inventor before: Zhu Pengyu Inventor before: Long Yang Inventor before: Yuan Junjie Inventor before: Lin Chungui Inventor before: Zhu Shuifang |
|
| COR | Change of bibliographic data | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161116 Termination date: 20180620 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |