CN115261200A

CN115261200A - Sampling device of nucleic acid extraction instrument and use method thereof

Info

Publication number: CN115261200A
Application number: CN202210895753.8A
Authority: CN
Inventors: 徐子凯; 龙智勇
Original assignee: Jinan Xinen Medical Technology Co ltd
Current assignee: Jinan Xinen Medical Technology Co ltd
Priority date: 2022-07-27
Filing date: 2022-07-27
Publication date: 2022-11-01

Abstract

The invention discloses a sampling device of a nucleic acid extraction instrument and a using method thereof, and the sampling device comprises a first fixing plate, a first one-way valve, a second fixing plate, a grab handle, an electric push rod, a liquid storage plate, a separation bin and a second one-way valve, wherein the first fixing plate and the second fixing plate are respectively arranged on two sides of the lower end of the liquid storage plate, the electric push rod is arranged on the upper surface of the liquid storage plate, and the grab handle is arranged on one side of the liquid storage plate, which is away from the first fixing plate; the first check valve of first fixed plate inside embedding installation, first fixed plate lower surface is provided with the first straw corresponding with first check valve, the second fixed plate deviates from first fixed plate one end lower surface and is provided with the second straw, the second check valve that the equidistance distributes is installed in second fixed plate one end embedding, stroke storehouse and partition bin have been seted up respectively to the liquid storage inboard. The invention can transfer samples in the nucleic acid sample tubes to the notches of the sample processing plate for the nucleic acid extractor in batches, thereby accelerating the sampling efficiency of the samples.

Description

Sampling device of nucleic acid extraction instrument and use method thereof

Technical Field

The invention relates to the technical field of nucleic acid sampling, in particular to a sampling device of a nucleic acid extraction instrument and a using method thereof.

Background

The nucleic acid extractor is an instrument which automatically finishes the work of extracting the nucleic acid of a sample by using a matched nucleic acid extracting reagent. The method is widely applied to various fields of disease control centers, clinical disease diagnosis, blood transfusion safety, forensic medicine identification, environmental microorganism detection, food safety detection, animal husbandry, molecular biology research and the like.

Before nucleic acid extraction, the staff need be with the sample transfer of the nucleic acid sample pipe to the specific storage tube that the nucleic acid extraction appearance used, and the bore of the storage tube that the nucleic acid extraction appearance used is littleer, and distributes more densely, and traditional mode of operation is that the staff absorbs the nucleic acid sample one by one and transfers to the compartment of storage tube in, and the effect of transferring and extracting the sample can receive the influence.

Through massive search, the prior art is found, and the publication number is as follows: CN114517144A discloses a nucleic acid extraction apparatus and a nucleic acid extraction method, which can improve the automation degree and is beneficial to reducing the cost. The nucleic acid extractor comprises a bracket, a containing table, a first position sensor assembly, a camera, a label identification device and a controller; the accommodating table is used for accommodating the deep hole plate; a sample label is arranged on the side surface of the deep hole plate and used for indicating information of a sample associated with the deep hole plate; the first position sensor assembly is used for sensing whether the deep hole plate is accommodated in place or not; when the deep hole plate is accommodated in place, the sample label is aligned with a light inlet of the camera, and the camera is used for collecting images containing the sample label; the label identification device is used for receiving the image and identifying a sample label in the image; the controller is used for receiving the identification result, determining the category of the sample based on the identification result, and acquiring a target nucleic acid extraction program corresponding to the category of the sample from the stored multiple nucleic acid extraction programs, wherein the target nucleic acid extraction program is used for realizing the nucleic acid extraction of the sample.

In summary, before nucleic acid extraction is performed by the conventional nucleic acid extraction instrument, samples in a nucleic acid sample tube need to be sampled, and the samples are placed in a dense grid-shaped compartment dedicated to the nucleic acid extraction instrument, so as to perform an efficient nucleic acid detection effect.

Disclosure of Invention

The present invention is directed to a sampling device of a nucleic acid extraction apparatus and a method for using the same, which solves the above problems of the prior art.

In order to achieve the purpose, the invention provides the following technical scheme: a sampling device of a nucleic acid extraction instrument and a using method thereof comprise a first fixing plate, a first one-way valve, a second fixing plate, a grab handle, an electric push rod, a liquid storage plate, a separation bin and a second one-way valve, wherein the first fixing plate and the second fixing plate are respectively arranged on two sides of the lower end of the liquid storage plate, the electric push rod is arranged on the upper surface of the liquid storage plate, and the grab handle is arranged on one side of the liquid storage plate, which is away from the first fixing plate;

the installation of the inside embedding of first fixed plate is the first check valve that the rectangular array distributes, first fixed plate lower surface is provided with the first straw corresponding with first check valve, the second fixed plate deviates from first fixed plate one end lower surface and is provided with the second straw that is linear arrangement, the second check valve that the equidistance distributes is installed in second fixed plate one end embedding, stroke storehouse and partition storehouse have been seted up respectively to the stock solution inboard.

Preferably, the upper end and the lower end of the first one-way valve respectively penetrate through the upper surface and the lower surface of the first fixing plate, and the upper end of the first suction pipe is inserted into the outer wall of the lower end of the first one-way valve;

the partition bin is provided with a connecting nozzle which penetrates through the outer wall of the liquid storage plate in the middle, and a hose is connected between the upper end of the first one-way valve and the connecting nozzle.

Preferably, the second fixing plate is internally provided with uniformly distributed communicating holes, and two ends of each communicating hole are respectively communicated with the upper end of the second suction pipe and one end of the second one-way valve;

a liquid discharge hole is formed in the inner wall of one side of the bottom end of the partition bin, and one end of the second one-way valve is inserted into the liquid discharge hole.

Preferably, the buckle that the equidistance symmetric distribution all is provided with in reservoir plate lower extreme both sides, and the draw-in groove with buckle looks adaptation is all seted up on the relative one end surface of first fixed plate and second fixed plate.

Preferably, the lower end of the electric push rod penetrates through the stroke bin, the lower end of the electric push rod is connected with a synchronizing rod, pistons distributed at equal intervals are arranged at the lower end of the synchronizing rod, and the lower ends of the pistons are all slidably inserted into the separation bin.

Preferably, the surface of one side of the liquid storage plate is provided with first fixed blocks which are symmetrically distributed, and the surface of one side of the grab handle is provided with second fixed blocks which are symmetrically distributed;

the first fixing block and the second fixing block are mutually embedded, and a bolt is inserted at the connecting position.

Preferably, one end of the grab handle is connected with a power line, and a button is embedded and installed on the lower surface of the grab handle;

the electric push rod is characterized in that a socket is arranged on the upper surface of the grab handle, a flat cable is connected to the bottom end of the electric push rod, and a plug matched with the socket is arranged at the tail end of the flat cable.

Preferably, the power line is mutually communicated with circuits between the button and the socket and used for supplying power to the electric push rod, and the button is used for controlling the bidirectional stroke of the electric push rod.

Preferably, the first suction pipes, the second suction pipes and the separation bin are the same in number, the one-way flow direction of the first one-way valve is from the first suction pipes to the separation bin, and the one-way flow direction of the second one-way valve is from the separation bin to the second suction pipes.

Preferably, the using method comprises the following steps:

s1: the medical staff places the nucleic acid sample pipe support under the first fixing plate, inserts the first suction tube into the nucleic acid sample pipe, controls the electric push rod to contract through the button, raises the piston along the interior of the separation bin, enables the interior of the separation bin to form negative pressure, and enables the sample in the nucleic acid sample pipe to enter the separation bin through the first one-way valve and the hose;

s2: medical personnel inhale the inside back of compartment with the sample in the nucleic acid sample pipe, correspond second straw and sample analysis pipe, and medical personnel presses the button, and control electric putter descends the synchronizing bar, drives the piston and pushes down along the compartment is inside, with the inside high pressure that forms of compartment, with the sample through second check valve and intercommunicating pore injection second straw.

Compared with the prior art, the invention has the beneficial effects that: the specification of the first fixing plate, the second fixing plate and the liquid storage plate is selected according to the number of the sample tubes stored in the nucleic acid sample rack, the number of the first suction tubes, the number of the second suction tubes and the number of the separation bins can be consistent with the number of the sample tubes in the nucleic acid sample rack, in the actual operation process, the samples can be sucked into the separation bins in sequence through the one-way circulation effect of the first check valve and the second check valve, and the samples are injected into the grid grooves in batches through the second suction tubes, so that the problem that the gap between the grid grooves and the caliber and the gap between the nucleic acid sample tubes are different, the sampling and the transferring of the nucleic acid samples can be carried out only by adopting the suction tubes one by one in the process of sampling and transferring the nucleic acid samples, and the efficiency of sampling and transferring the nucleic acid samples is effectively improved.

Drawings

FIG. 1 is a schematic front view of the present invention;

fig. 2 is a schematic cross-sectional view of the first fixing plate and the second fixing plate of the present invention;

FIG. 3 is a schematic cross-sectional view of a liquid storage plate according to the present invention;

FIG. 4 is a schematic diagram of a top view distribution structure of the hose and the communicating tube according to the present invention;

FIG. 5 is a schematic sectional view of a liquid storage plate according to the present invention;

FIG. 6 is a schematic cross-sectional view of the bottom of the liquid storage plate of the present invention.

In the figure: 1. a first fixing plate; 2. a first straw; 3. a first check valve; 4. a second fixing plate; 5. a second straw; 6. a communicating hole; 7. a power line; 8. a button; 9. a handle; 10. an electric push rod; 11. separating bins; 12. a liquid storage plate; 13. a hose; 14. a connecting nozzle; 15. a second check valve; 16. a stroke bin; 17. a synchronization lever; 18. a piston; 19. a first fixed block; 20. a second fixed block; 21. a bolt; 22. a socket; 23. a plug; 24. arranging wires; 25. a liquid discharge hole; 26. a card slot; 27. and (5) buckling.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front", "rear", "both ends", "one end", "the other end", and the like indicate orientations or positional relationships based on orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be configured in a specific orientation, and operate, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless explicitly stated or limited otherwise, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, such as "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to fig. 1 to 6, the present invention provides six embodiments:

example one

The utility model provides a nucleic acid draws instrument sampling device, including first fixed plate 1, first check valve 3, second fixed plate 4, grab handle 9, electric putter 10, stock solution board 12, separate storehouse 11 and second check valve 15, stock solution board 12 is inside to have seted up stroke storehouse 16 and separate storehouse 11 respectively, stock solution board 12 lower extreme both sides are provided with first fixed plate 1 and second fixed plate 4 respectively, stock solution board 12 lower extreme both sides all are provided with the buckle 27 of equidistance symmetric distribution, and first fixed plate 1 and the relative one end surface of second fixed plate 4 all offer with the draw-in groove 26 of buckle 27 looks adaptation.

First fixed plate 1 and second fixed plate 4 respectively through draw-in groove 26 and the buckle 27 of stock solution board 12 lower extreme to combining, can be with first fixed plate 1 and second fixed plate 4 stable installation in stock solution board 12 both sides, do benefit to follow-up use and dismantlement.

Example two:

the upper surface of the liquid storage plate 12 is provided with an electric push rod 10, a grab handle 9 is arranged on one side, deviating from the first fixing plate 1, of the liquid storage plate 12, the lower end of the electric push rod 10 penetrates through the interior of the stroke bin 16, the lower end of the electric push rod 10 is connected with a synchronizing rod 17, the lower end of the synchronizing rod 17 is provided with pistons 18 distributed at equal intervals, and the lower ends of the pistons 18 are all slidably inserted into the interior of the separation bin 11.

The surface of one side of the liquid storage plate 12 is provided with first fixed blocks 19 which are symmetrically distributed, the surface of one side of the grab handle 9 is provided with second fixed blocks 20 which are symmetrically distributed, the first fixed blocks 19 and the second fixed blocks 20 are mutually embedded, and the connection position is inserted with a bolt 21. The bolt 21 can connect the first fixing block 19 and the second fixing block 20, so as to facilitate the installation and the removal of the handle 9 and the liquid storage plate 12.

One end of the grab handle 9 is connected with a power cord 7, a button 8 is embedded in the lower surface of the grab handle 9, a socket 22 is arranged on the upper surface of the grab handle 9, a flat cable 24 is connected to the bottom end of the electric push rod 10, and a plug 23 matched with the socket 22 is arranged at the tail end of the flat cable 24. The power cord 7 is in circuit communication with the button 8 and the socket 22 for supplying power to the power putter 10, and the button 8 is used for controlling the bidirectional stroke of the power putter 10. The button 8 is of a three-gear control specification and corresponds to the pushing, stopping and retracting of the electric push rod 10 respectively, and the arrangement of the socket 22 can facilitate the connection between the electric push rod 10 corresponding to the upper ends of different liquid storage plates 12 and the button 8 and a power supply.

Example three:

the first check valve 3 that the inside embedding installation of first fixed plate 1 is rectangular array and distributes, 1 lower surface of first fixed plate is provided with the first straw 2 corresponding with first check valve 3, the upper and lower both ends of first check valve 3 run through the upper and lower surface of first fixed plate 1 respectively, and first straw 2 upper end is pegged graft in 3 lower extreme outer walls of first check valve, 11 intermediate position departments of compartment all imbed and install the connector 14 that runs through the outer wall of liquid storage board 12, be connected with hose 13 between 3 upper ends of first check valve and the connector 14, medical personnel arrange the nucleic acid sample pipe support in 1 below of first fixed plate, peg graft first straw 2 to in the nucleic acid sample pipe, through the shrink of button 8 control electric putter 10, with piston 18 along the inside rising of compartment 11, make the inside negative pressure that forms of compartment 11, get into the compartment 11 through first check valve 3 and hose 13 with the sample in the nucleic acid sample pipe.

Example four:

the lower surface of one end of a second fixing plate 4, which deviates from the first fixing plate 1, is provided with second suction pipes 5 which are linearly arranged, one end of the second fixing plate 4 is embedded with second check valves 15 which are equidistantly distributed, communication holes 6 which are uniformly distributed are formed in the second fixing plate 4, two ends of each communication hole 6 are respectively communicated with the upper end of each second suction pipe 5 and one end of each second check valve 15, a liquid discharge hole 25 is formed in the inner wall of one side of the bottom end of each separation bin 11, one end of each second check valve 15 is inserted into the liquid discharge hole 25, the number of the first suction pipes 2, the number of the second suction pipes 5 and the number of the separation bins 11 are the same, the unidirectional flow direction of the first check valves 3 is from the first suction pipes 2 to the separation bins 11, the unidirectional flow direction of the second check valves 15 is from the separation bins 11 to the second suction pipes 5, medical workers suck samples in the nucleic acid sample tubes into the separation bins 11 and then correspond to the second suction pipes 5, the sample analysis pipes through the second check valves 15, and press a button 8, an electric push rod 10 is controlled to descend, and drive a piston 18 to press down along the insides of the separation bins 11 to form high pressure in the separation bins 11, and inject the sample tubes into the second suction pipes through the second check valves 15 and the second check valves 15.

Example five:

a method for using a sampling device of a nucleic acid extraction instrument comprises the following steps:

s1: a medical worker places the nucleic acid sample tube rack below the first fixing plate 1, inserts the first suction tube 2 into the nucleic acid sample tube, controls the electric push rod 10 to contract through the button 8, raises the piston 18 along the inside of the separation bin 11, enables the inside of the separation bin 11 to form negative pressure, and enables a sample in the nucleic acid sample tube to enter the separation bin 11 through the first one-way valve 3 and the hose 13;

s2: after a medical worker sucks a sample in a nucleic acid sample tube into a separation bin 11, a second suction tube 5 corresponds to a sample analysis tube, the medical worker presses a button 8, an electric push rod 10 is controlled to descend a synchronous rod 17, a piston 18 is driven to press down along the interior of the separation bin 11, high pressure is formed in the interior of the separation bin 11, the sample is injected into the second suction tube 5 through a second one-way valve 15 and a communication hole 6, specifications of a first fixing plate 1, a second fixing plate 4 and a liquid storage plate 12 can be selected and matched according to the number of the sample tubes stored in a nucleic acid sample rack, the number of the first suction tube 2, the number of the second suction tube 5 and the number of the separation bin 11 can be consistent with the number of the sample tubes in the nucleic acid sample tube rack, in the actual operation process, the sample can be sucked into the separation bin 11 in sequence through the one-way circulation effect of the first one-way valve 3 and the second one-way valve 15, the sample can be injected into grid slots in batches through the second suction tube 5, the problem that the gap between the grid slots and the aperture and the gap between the nucleic acid sample tubes are different is solved, the sample transfer process, and the sample transfer efficiency is improved only by adopting the effective sample transfer process.

Example six:

the whole cleaning process of the sampling device comprises the following steps:

the worker respectively places the two ends of the first suction pipe 2 and the second suction pipe 5 in the two containers, the container where the first suction pipe 2 is located is filled with the sterilized alcohol, the power is switched on, the piston 18 is lifted along the inside of the partition bin 11 through the contraction of the electric push rod 10, negative pressure is formed inside the partition bin 11, and the sterilized alcohol enters the partition bin 11 through the first one-way valve 3 and the hose 13; and is injected into the second suction pipe 5 through the second check valve 15 and the communication hole 6, and the second suction pipe 5 is sprayed out and is reciprocated for 5 to 10 times, thereby realizing the internal sterilization and cleaning operation.

The working principle is as follows: a medical worker places the nucleic acid sample tube rack below the first fixing plate 1, inserts the first suction tube 2 into the nucleic acid sample tube, controls the electric push rod 10 to contract through the button 8, raises the piston 18 along the inside of the separation bin 11, enables the inside of the separation bin 11 to form negative pressure, and enables a sample in the nucleic acid sample tube to enter the separation bin 11 through the first one-way valve 3 and the hose 13; medical personnel inhale the sample in the nucleic acid sample pipe and divide 11 inside backs, correspond second straw 5 and sample analysis pipe, and medical personnel press the button 8, and control electric putter 10 descends synchronizing bar 17, drives piston 18 and pushes down along dividing 11 inside in storehouse, will divide 11 inside formation high pressures in storehouse, pours into the sample into second straw 5 through second check valve 15 and intercommunicating pore 6.

Can carry out the specification of apolegamy first fixed plate 1, second fixed plate 4 and stock solution board 12 according to the sample tube quantity of storage in the nucleic acid sample frame, can unify first straw 2, second straw 5 and the sample tube quantity in 11 quantity and the nucleic acid sample pipe support of compartment, in the actual operation process, through the one-way circulation effect of first check valve 3 and second check valve 15, can inhale the compartment 11 with the sample in proper order, and inject the sample into the grid groove in batches through second straw 5, it all has the difference to have solved the bore and the interval between the interval of grid groove and the nucleic acid sample pipe, carry out nucleic acid sample between the two and sample and the transfer in-process, only can adopt the straw to carry out the problem of taking a sample and transferring one by one, the efficiency of nucleic acid sample and transferring has effectively been improved.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. The utility model provides a nucleic acid extraction instrument sampling device, includes first fixed plate (1), first check valve (3), second fixed plate (4), grab handle (9), electric putter (10), stock solution board (12), separate storehouse (11) and second check valve (15), its characterized in that: a first fixing plate (1) and a second fixing plate (4) are respectively arranged on two sides of the lower end of the liquid storage plate (12), an electric push rod (10) is arranged on the upper surface of the liquid storage plate (12), and a grab handle (9) is arranged on one side, away from the first fixing plate (1), of the liquid storage plate (12);

first fixed plate (1) inside embedding installation is first check valve (3) that the rectangle array distributes, first fixed plate (1) lower surface is provided with first straw (2) corresponding with first check valve (3), second fixed plate (4) deviate from first fixed plate (1) one end lower surface and are provided with second straw (5) that are linear arrangement, second check valve (15) that the equidistance distributes are installed in second fixed plate (4) one end embedding, stroke storehouse (16) and separate storehouse (11) have been seted up respectively to liquid storage plate (12) inside.

2. The sampling device of claim 1, wherein: the upper end and the lower end of the first check valve (3) respectively penetrate through the upper surface and the lower surface of the first fixing plate (1), and the upper end of the first suction pipe (2) is inserted into the outer wall of the lower end of the first check valve (3);

divide compartment (11) intermediate position department all imbeds and installs connection mouth (14) that run through liquid storage plate (12) outer wall, be connected with hose (13) between first check valve (3) upper end and connection mouth (14).

3. The sampling device of claim 1, wherein: the second fixing plate (4) is internally provided with uniformly distributed communicating holes (6), and two ends of each communicating hole (6) are respectively communicated with the upper end of the second suction pipe (5) and one end of the second one-way valve (15);

a liquid discharge hole (25) is formed in the inner wall of one side of the bottom end of the partition bin (11), and one end of the second one-way valve (15) is inserted into the liquid discharge hole (25).

4. The sampling device of claim 1, wherein: liquid storage plate (12) lower extreme both sides all are provided with buckle (27) of equidistance symmetric distribution, and first fixed plate (1) and second fixed plate (4) relative one end surface all offer with draw-in groove (26) of buckle (27) looks adaptation.

5. The sampling device of claim 1, wherein: the lower end of the electric push rod (10) penetrates through the interior of the stroke bin (16), the lower end of the electric push rod (10) is connected with a synchronizing rod (17), pistons (18) distributed at equal intervals are arranged at the lower end of the synchronizing rod (17), and the lower ends of the pistons (18) are inserted into the separation bin (11) in a sliding mode.

6. The sampling device of claim 1, wherein: the surface of one side of the liquid storage plate (12) is provided with first fixing blocks (19) which are symmetrically distributed, and the surface of one side of the grab handle (9) is provided with second fixing blocks (20) which are symmetrically distributed;

the first fixing block (19) and the second fixing block (20) are mutually embedded, and a bolt (21) is inserted at the connecting position.

7. The sampling device of claim 1, wherein: one end of the grab handle (9) is connected with a power line (7), and a button (8) is embedded and installed on the lower surface of the grab handle (9);

the upper surface of the grab handle (9) is provided with a socket (22), the bottom end of the electric push rod (10) is connected with a flat cable (24), and the tail end of the flat cable (24) is provided with a plug (23) matched with the socket (22).

8. The sampling device of claim 7, wherein: the power cord (7) is mutually communicated with the button (8) and the socket (22) through circuits and is used for supplying power to the electric push rod (10), and the button (8) is used for controlling the bidirectional stroke of the electric push rod (10).

9. The sampling device of claim 1, wherein: the number of the first suction pipes (2), the number of the second suction pipes (5) and the number of the separation bins (11) are the same, the one-way flowing direction of the first one-way valve (3) is from the first suction pipes (2) to the separation bins (11), and the one-way flowing direction of the second one-way valve (15) is from the separation bins (11) to the second suction pipes (5).

10. The use method of a sampling device of a nucleic acid extraction apparatus as claimed in claims 1 to 9, wherein: the using method comprises the following steps:

s1: a medical worker places a nucleic acid sample tube support below a first fixing plate (1), inserts a first suction tube (2) into the nucleic acid sample tube, controls an electric push rod (10) to contract through a button (8), and raises a piston (18) along the inside of a separation bin (11) to form negative pressure inside the separation bin (11), so that a sample in the nucleic acid sample tube enters the separation bin (11) through a first one-way valve (3) and a hose (13);

s2: medical personnel inhale the sample in the nucleic acid sample pipe and divide storehouse (11) inside back, correspond second straw (5) and sample analysis pipe, and medical personnel press down button (8), and control electric putter (10) descend synchronizing bar (17), drive piston (18) and push down along dividing storehouse (11) inside, will divide storehouse (11) inside formation high pressure, pour into the sample in second straw (5) through second check valve (15) and intercommunicating pore (6).