CN116254179A - Magnetic bead method nucleic acid extraction instrument - Google Patents

Magnetic bead method nucleic acid extraction instrument Download PDF

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Publication number
CN116254179A
CN116254179A CN202310445984.3A CN202310445984A CN116254179A CN 116254179 A CN116254179 A CN 116254179A CN 202310445984 A CN202310445984 A CN 202310445984A CN 116254179 A CN116254179 A CN 116254179A
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plate
gear ring
instrument main
nucleic acid
main body
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Granted
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CN202310445984.3A
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Chinese (zh)
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CN116254179B (en
Inventor
郑志强
闫锦锦
匡志宏
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Hangzhou Maozhi Biotechnology Co ltd
Wuhu Maozhi Biotechnology Co ltd
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Hangzhou Maozhi Biotechnology Co ltd
Wuhu Maozhi Biotechnology Co ltd
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Priority to CN202310445984.3A priority Critical patent/CN116254179B/en
Publication of CN116254179A publication Critical patent/CN116254179A/en
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Publication of CN116254179B publication Critical patent/CN116254179B/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
    • A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
    • A61L2202/10—Apparatus features
    • A61L2202/15—Biocide distribution means, e.g. nozzles, pumps, manifolds, fans, baffles, sprayers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
    • A61L2202/20—Targets to be treated
    • A61L2202/23—Containers, e.g. vials, bottles, syringes, mail
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
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  • Physics & Mathematics (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)

Abstract

The invention relates to the field of nucleic acid extraction, in particular to a magnetic bead method nucleic acid extraction instrument, which comprises an instrument main body, wherein a base is arranged in the instrument main body, a limit frame is fixedly arranged on the base, a lifting track is arranged in the instrument main body, a lower frame and an upper frame are connected in a driving manner in the lifting track, a magnetic rod is arranged on the upper frame, a movable support is arranged in the instrument main body in a lifting manner, a cleaning sleeve is arranged on the movable support, a compression plate is arranged on the movable support, an additional box is fixedly connected to the instrument main body, a movable piece is movably arranged on the base, a rack is fixedly connected to the movable piece, a gear ring structure with a unidirectional transmission function is arranged in the additional box, a transmission shaft is arranged in the center of the gear ring structure, a knocking plate is connected to the transmission shaft, a disinfectant storage structure for pressing discharged water is arranged on the additional box, and a spray head is connected to the disinfectant storage structure; the invention can achieve the purpose of automatically cleaning the internal space of the extractor and avoiding the pollution of the extracted sample.

Description

Magnetic bead method nucleic acid extraction instrument
Technical Field
The invention relates to the technical field of nucleic acid extraction, in particular to a magnetic bead method nucleic acid extraction instrument.
Background
The magnetic bead method is to crack cell tissue sample with the cracking liquid, adsorb nucleic acid molecule to the surface of magnetic particle, retain protein and other impurity in solution, move the magnetic bead with nucleic acid into different reagent tank, stir and mix the liquid fast repeatedly, crack cell, adsorb nucleic acid, wash and elute to obtain pure nucleic acid.
The traditional magnetic bead method for extracting nucleic acid is performed manually, and the existing nucleic acid extractor can automatically extract nucleic acid according to the steps, so that the extraction efficiency is improved. At present, when a nucleic acid extraction instrument is used, careful disinfection and cleaning of the internal space of the instrument are required between experiments of each batch to avoid intermixing between samples. However, the existing nucleic acid extractor is inconvenient to clean, generally is sprayed with disinfection alcohol manually, and is wiped finely, so that dead angles are easy to clean due to carelessness of people, and sample pollution is caused.
Disclosure of Invention
The invention aims to provide a magnetic bead method nucleic acid extractor, so as to achieve the purposes of automatically cleaning the inner space of the extractor and avoiding the pollution of an extracted sample, and solve the problems in the prior art.
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides a magnetic bead method nucleic acid extraction appearance, includes the instrument main part, be provided with the base in the instrument main part, and fixed mounting has spacing frame on the base, be provided with the spring pin in the spacing frame, be provided with lifting rail in the instrument main part, and drive connection has lower frame and upper frame in the lifting rail, be provided with bar magnet cover fastener on the lower frame, and be provided with the bar magnet on the upper frame, elevating mounting has movable support in the instrument main part, and is provided with clean cover on the movable support, and fixedly connected with reset spring on the movable support, be provided with the pressure receiving plate on the movable support, and fixed connection has the additional case on the instrument main part, and the pressure receiving plate stretches into in the additional case, movable mounting has the moving part on the base, and fixedly connected with rack on the moving part, be provided with the ring gear structure that has unidirectional transmission function in the additional case, and the ring gear structure center is provided with the transmission shaft, be connected with the knocking board on the additional case and press out water storage structure, and the disinfection liquid storage structure is connected with the shower nozzle, be provided with the rotation mounting panel, and the top surface through the clamp plate, the clamp plate is connected with the rope through the fixed connection with the fixed connection rope on the fixed bearing, the top surface is connected with the clamp plate through the fixed connection.
Preferably, the base is arranged at the bottom of the instrument main body cavity, two deep hole plate grooves are formed in the limiting frame, the spring pins are located at two sides of the deep hole plate grooves, and the lifting rail is arranged on the side wall of the instrument main body cavity.
Preferably, the lower frame and the upper frame are arranged in parallel, the magnetic rod sleeve clamping piece is arranged at the bottom of the lower frame, the magnetic rods are vertically arranged at the bottom of the upper frame, the movable support is located at the magnetic rods, the cleaning sleeve is sleeved on the magnetic rods, and the reset spring is connected to the top of the cavity of the instrument body.
Preferably, a cavity is formed in the base, the movable piece is linearly and slidably mounted in the cavity in a limiting mode, the movable piece is arranged on the surface of the base in a protruding mode, the rack is horizontally arranged, and the rack extends into the additional box from the base.
Preferably, the gear ring structure comprises a gear ring which is arranged at the bottom of the additional box in a limiting rotation mode, the bottom of the gear ring is fixedly connected with a scroll spring, a push plate is rotatably arranged on the inner wall of the gear ring, a stop block is fixedly arranged on the inner wall of the gear ring, and an outer toothed plate is annularly arranged on the transmission shaft.
Preferably, the gear ring and the transmission shaft are concentrically arranged, one end of the spiral spring is connected with the gear ring, the other end of the spiral spring is connected with the inner wall of the additional box, the push plate is connected with the external gear plate, the stop block is in contact connection with one side of the push plate, and the gear ring is meshed with the rack.
Preferably, the disinfectant storage structure comprises a disinfectant tank arranged on the additional tank, a liquid outlet tank is arranged on the disinfectant tank, a pipeline is connected to the liquid outlet tank and communicated with the spray head, and a pressing pump is arranged on the liquid outlet tank.
Preferably, the spray head is arranged on the inner wall of the cavity of the instrument main body, and the knocking plate is positioned on the side face of the pressing pump.
Preferably, the mounting plate is located between the additional box and the instrument main body, and the rope loop is limited to rotate and is installed at the bottom of the mounting plate, the guide rod is vertically arranged on the top surface of the mounting plate, and the pull rope penetrates through the mounting plate and is connected to the bottom surface of the pressing plate.
Compared with the prior art, the invention has the beneficial effects that:
1. the nucleic acid extraction instrument can automatically finish the nucleic acid extraction work, is provided with the cleaning structure corresponding to the magnetic rods one by one, is also provided with the sterilizing liquid spraying structure, can mutually cooperate to finish the sterilization and cleaning in the instrument, can push the movable part when the deep hole plate is inserted into the limiting frame, drives the rack to move towards the direction of the additional box, can be connected with the gear ring structure when the rack stretches into the additional box, can store elastic potential energy in the gear ring structure, can provide power for the subsequent sterilization work, can extract the deep hole plate after the extraction work is finished, enables the elastic potential energy to be released, starts the sterilization, automatically performs the sterilization work without manual operation, and avoids adverse effects caused by manual negligence.
2. The gear ring structure can drive the transmission shaft to rotate unidirectionally, when the transmission shaft rotates, the disinfection solution storage structure is driven to output disinfection solution in the instrument main body, after the deep hole plate and samples in the deep hole plate are taken out, the instrument main body cavity is disinfected automatically, so that the samples are prevented from being polluted, the next extraction work is prevented from being influenced, meanwhile, when the transmission shaft rotates, the transmission shaft is connected with the rope ring through the unidirectional bearing, the magnetic rod cleaning structure can be driven, the cleaning of the magnetic rod is completed by matching with the disinfection solution, the reset of the cleaning structure does not influence the transmission shaft due to the existence of the unidirectional bearing, the gear ring structure and the disinfection solution storage structure are prevented from being influenced, and the whole disinfection work is conducted automatically through the linked transmission structure, so that the operation is quite convenient.
Drawings
FIG. 1 is a first schematic view of the overall structure of the apparatus of the present invention.
Fig. 2 is a second schematic view of the overall structure of the apparatus of the present invention.
FIG. 3 is a schematic view of the internal structure of the apparatus of the present invention.
Fig. 4 is a schematic view of a base structure of the present invention.
Fig. 5 is a schematic view of the magnetic bar mounting structure of the present invention.
Fig. 6 is a schematic view of the structure of the magnetic rod and the cleaning sleeve of the present invention.
Fig. 7 is a schematic view of the structure of the additional tank of the present invention.
Fig. 8 is a first schematic view of the structure of the transmission part of the present invention.
Fig. 9 is a second schematic view of the structure of the transmission part of the present invention.
Fig. 10 is a schematic view of the structure of the transmission shaft of the present invention.
Fig. 11 is a schematic view of the structure of the ring gear of the present invention.
In the figure: the device comprises an instrument main body 1, a base 2, a limit frame 3, a spring pin 4, a lifting track 5, a lower frame 6, a magnetic rod sleeve clamping piece 7, an upper frame 8, a magnetic rod 9, a movable support 10, a cleaning sleeve 11, a return spring 12, a pressure receiving plate 13, an additional box 14, a movable piece 15, a rack 16, a gear ring 17, a spiral spring 18, a push plate 19, a stop block 20, a transmission shaft 21, an external gear plate 22, a knocking plate 23, a disinfectant tank 24, a liquid outlet groove 25, a pipeline 26, a spray head 27, a pressing pump 28, a mounting plate 29, a rope ring 30, a one-way bearing 31, a pull rope 32, a guide rod 33 and a pressure plate 34.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and detailed description, wherein it is to be understood that, under the precondition of no conflict, new embodiments can be formed by any combination of the embodiments or technical features described below, and it should be understood that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1 to 11, the present invention provides a technical solution: the utility model provides a magnetic bead method nucleic acid extraction appearance, including instrument main part 1, be provided with base 2 in the instrument main part 1, and fixed mounting has spacing frame 3 on the base 2, be provided with spring pin 4 in the spacing frame 3, be provided with elevating track 5 in the instrument main part 1, and drive connection has lower frame 6 and upper frame 8 in the elevating track 5, be provided with bar magnet cover fastener 7 on the lower frame 6, and be provided with bar magnet 9 on the upper frame 8, install movable support 10 in the instrument main part 1, and be provided with clean cover 11 on the movable support 10, and fixedly connected with reset spring 12 on the movable support 10, be provided with the clamp plate 13 on the movable support 10, and fixedly connected with additional case 14 on the instrument main part 1, and the clamp plate 13 stretches into in the additional case 14, movable mounting has movable member 15 on the base 2, and fixedly connected with rack 16 on the movable member 15, be provided with the ring gear structure with unidirectional transmission function in the additional case 14, and the ring gear structure center is provided with transmission shaft 21, be connected with on the transmission shaft 21 and strike plate 23, be provided with the disinfection liquid storage structure of pressing out water in the additional case 14, and the clamp plate 29 is connected with the clamp plate 32 through the clamp plate 29, and the clamp plate 30 is connected with the clamp plate 32 in the fixed connection 13, the top surface is connected with the clamp plate 32 through the clamp plate 29, the clamp plate is connected with the clamp plate 30 in the fixed connection 32, the clamp plate is connected with the clamp plate is fixed to the clamp plate is 30 in the clamp plate is fixed to the clamp plate is connected with the clamp plate is 30.
The base 2 is arranged at the bottom of the cavity of the instrument main body 1, two deep hole plate grooves are formed in the limiting frame 3, the spring pins 4 are located on two sides of the deep hole plate grooves, and the lifting rail 5 is arranged on the side wall of the cavity of the instrument main body 1.
The nucleic acid extraction instrument can automatically finish the nucleic acid extraction work, when in use, a deep hole plate filled with a sample and magnetic beads is arranged on the base 2, the deep hole plate is fixed through the limiting frame 3 with the spring pin, the magnetic rod sleeve is arranged through the magnetic rod sleeve clamping piece 7, the lifting track 5 can drive the lower frame 6 and the upper frame 8 to move, and the magnetic rod sleeve and the magnetic rod 9 on the lifting track can be sent into the deep hole plate to perform the magnetic bead extraction work.
The lower frame 6 and the upper frame 8 are arranged in parallel, the magnetic rod sleeve clamping piece 7 is arranged at the bottom of the lower frame 6, the magnetic rods 9 are vertically arranged at the bottom of the upper frame 8, the movable support 10 is located at the magnetic rods 9, the cleaning sleeve 11 is sleeved on the magnetic rods 9, and the reset spring 12 is connected to the top of the cavity of the instrument main body 1.
The invention is provided with the cleaning sleeves 11 aiming at the magnetic rods 9, the cleaning sleeves 11 are in one-to-one correspondence with the magnetic rods 9, and the cleaning sleeves 11 are arranged on the movable support 10, so that the disinfection and cleaning work of the magnetic rods 9 can be completed through the up-and-down movement of the movable support 10.
The base 2 is provided with a cavity, the movable piece 15 is arranged in the cavity in a linear limiting sliding manner, the movable piece 15 is arranged on the surface of the base 2 in a protruding manner, the rack 16 is horizontally arranged, and the rack 16 extends into the additional box 14 from the base 2.
The sterilization and cleaning of the cavity in the instrument body 1 is carried out by means of the installation of the deep hole plate, and when the deep hole plate is inserted into the limit frame 3, the movable piece 15 can be pushed to drive the rack 16 to move towards the additional box 14.
The gear ring structure comprises a gear ring 17 which is arranged at the bottom of the additional box 14 in a limiting rotation manner, the bottom of the gear ring 17 is fixedly connected with a scroll spring 18, a push plate 19 is rotatably arranged on the inner wall of the gear ring 17, a stop block 20 is fixedly arranged on the inner wall of the gear ring 17, and an outer toothed plate 22 is annularly arranged on a transmission shaft 21.
When the rack 16 extends into the additional tank 14, it can be connected with the gear ring structure, and the elastic potential energy is accumulated in the gear ring structure to provide power for the subsequent disinfection work, and after the extraction work is completed, the deep hole plate can be extracted, so that the elastic potential energy is released, and disinfection is started.
The gear ring 17 and the transmission shaft 21 are concentrically arranged, one end of the spiral spring 18 is connected with the gear ring 17, the other end is connected with the inner wall of the additional box 14, the push plate 19 is connected with the outer gear plate 22, the stop 20 is in contact connection with one side of the push plate 19, and the gear ring 17 is meshed with the rack 16.
When the rack 16 moves, the rack can be meshed with the gear ring 17 to drive the gear ring 17 to rotate, so as to push the spiral spring 18 to wind the spiral spring 18 to accumulate elastic potential energy, when the gear ring 17 rotates towards the side, the push plate 19 cannot be blocked by the stop block 20 to rotate, the push plate 19 cannot push the outer toothed plate 22, all the transmission shafts 21 cannot rotate, otherwise, when the deep hole plate is taken out and the spiral spring 18 releases potential energy, the gear ring 17 can be reversely rotated, and then the transmission shafts 21 can be driven to rotate.
The disinfectant storage structure comprises a disinfectant tank 24 arranged on the additional tank 14, a liquid outlet tank 25 is arranged on the disinfectant tank 24, a pipeline 26 is connected to the liquid outlet tank 25, the pipeline 26 is communicated with a spray head 27, and a pressing pump 28 is arranged on the liquid outlet tank 25.
When the transmission shaft 21 rotates, the pressing drive of the sterilizing liquid storage structure can be performed, so that the sterilizing liquid storage structure outputs sterilizing liquid in the instrument main body 1, and the sterilization of the cavity of the instrument main body 1 is automatically performed after the deep hole plate and the sample therein are taken out.
The shower head 27 is provided on the inner wall of the cavity of the instrument body 1, and the striking plate 23 is located on the side of the pressing pump 28.
The rotation of the transmission shaft 21 can drive the knocking plate 23 to rotate, the knocking plate 23 repeatedly collides on the pressing pump 28, disinfectant is pumped into the pipeline 26 from the disinfectant tank 24 and the liquid outlet groove 25 through the pressing pump 28, and is sprayed into the cavity of the instrument main body 1 from the spray head 27 connected with the pipeline 26, so that the cavity space is disinfected, and the sample is prevented from being polluted, so that the next extraction work is not influenced.
The mounting plate 29 is located between the additional box 14 and the instrument main body 1, and the rope loop 30 is installed at the bottom of the mounting plate 29 in a limiting rotation mode, the guide rod 33 is vertically arranged on the top surface of the mounting plate 29, and the pull rope 32 penetrates through the mounting plate 29 and is connected to the bottom surface of the pressing plate 34.
Meanwhile, when the transmission shaft 21 rotates, as the transmission shaft 21 is connected with the rope loop 30 through the one-way bearing 31, the transmission shaft 21 can drive the rope loop 30 to rotate, and when the rope loop 30 rotates, the pull rope 32 can be wound, the pressure generated by the pressure plate 34 is pulled by the pull rope 32 to push down the pressure plate 13, so that the movable support 10 overcomes the elasticity of the return spring 12 to move downwards, the cleaning sleeve 11 on the movable support can move, the cleaning sleeve can be matched with the disinfectant to finish the cleaning of the magnetic rod 9, after one-time cleaning is finished, the return spring 12 can reset and move upwards the movable support 10, the pressure plate 34 can also reset upwards, and the rope loop 30 is driven to rotate reversely through the pull rope 32, but when the rope loop 30 rotates reversely, the existence of the one-way bearing 31 can not influence the transmission shaft 21, and the transmission shaft 21 cannot rotate to cause the influence on the gear ring structure and the disinfectant storage structure.
The invention is used when in use: firstly, the nucleic acid extraction instrument of the invention can automatically complete the nucleic acid extraction work, when in use, a deep hole plate filled with samples and magnetic beads is arranged on a base 2, the deep hole plate is fixed through a limit frame 3 with a spring pin, a magnetic rod sleeve is arranged through a magnetic rod sleeve clamping piece 7, a lifting rail 5 can drive a lower frame 6 and an upper frame 8 to move, the magnetic rod sleeve and a magnetic rod 9 on the lifting rail 5 are sent into the deep hole plate to carry out the magnetic bead extraction work, the invention is provided with a cleaning sleeve 11 aiming at the magnetic rod 9, the cleaning sleeve 11 corresponds to the magnetic rod 9 one by one, the cleaning sleeve 11 is arranged on a movable bracket 10, the disinfection cleaning work of the magnetic rod 9 can be completed through the up-down movement of the movable bracket 10, the disinfection cleaning work of the cavity in the instrument main body 1 is carried out by means of the installation of a deep hole plate, when the deep hole plate is inserted into the limit frame 3, the movable piece 15 can be pushed, when the rack 16 is driven to move towards the direction of the additional box 14, the rack 16 can be connected with a gear ring structure to accumulate elastic potential energy in the gear ring structure to provide power for subsequent disinfection, after the extraction work is completed, the deep hole plate can be pulled out to release the elastic potential energy to start disinfection, when the rack 16 moves, the rack 16 can be meshed with the gear ring 17 to drive the gear ring 17 to rotate so as to push the scroll spring 18 to accumulate the elastic potential energy, when the gear ring 17 rotates towards the side, the push plate 19 can not be blocked by the stop block 20 to rotate, the push plate 19 can not push the outer toothed plate 22, all the transmission shafts 21 can not rotate, otherwise, when the deep hole plate is taken out and the scroll spring 18 releases potential energy, the gear ring 17 can reversely rotate so as to drive the transmission shafts 21 to rotate, when the transmission shaft 21 rotates, the pressing driving of the disinfectant storage structure can be carried out, so that the disinfectant storage structure outputs disinfectant in the instrument main body 1, the disinfection of the cavity of the instrument main body 1 is automatically carried out after the deep hole plate and samples in the deep hole plate are taken out, the rotation of the transmission shaft 21 can drive the knocking plate 23 to rotate, the knocking plate 23 repeatedly impacts on the pressing pump 28, the disinfectant is pumped into the pipeline 26 from the disinfectant tank 24 and the liquid outlet groove 25 through the pressing pump 28, the spray head 27 connected with the pipeline 26 sprays into the cavity of the instrument main body 1 to disinfect the cavity space, the samples are prevented from being polluted, the next extraction work is not influenced, meanwhile, when the transmission shaft 21 rotates, the transmission shaft 21 is connected with the rope ring 30 through the one-way bearing 31, the transmission shaft 21 can drive the loop 30 to rotate, and when the loop 30 rotates, the stay cord 32 can be wound, the pressure produced by the stay cord 32 pulls the pressing plate 34, the pressing plate 34 presses down the pressing plate 13, so that the movable support 10 overcomes the elasticity of the return spring 12 and moves downwards, the cleaning sleeve 11 on the movable support can move, the cleaning of the magnetic rod 9 is completed by matching with the disinfectant, the return spring 12 can reset and move upwards the movable support 10 after one-time cleaning is completed, the pressing plate 34 can also move upwards and reset, the loop 30 is driven to rotate reversely through the stay cord 32, but when the loop 30 rotates reversely, the transmission shaft 21 cannot be influenced due to the existence of the unidirectional bearing 31, and the transmission shaft 21 cannot rotate to influence the gear ring structure and the disinfectant storage structure.
The above embodiments are only preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, but various modifications can be made by those skilled in the art without inventive effort from the above concepts, and all modifications are within the scope of the present invention.

Claims (9)

1. The utility model provides a magnetic bead method nucleic acid extraction appearance, includes instrument main part (1), its characterized in that: the utility model discloses a single-direction impact type vibration-damping device, which is characterized in that a base (2) is arranged in an instrument main body (1), a limit frame (3) is fixedly arranged on the base (2), a spring pin (4) is arranged in the limit frame (3), a lifting track (5) is arranged in the instrument main body (1), a lower frame (6) and an upper frame (8) are connected in a driving manner in the lifting track (5), a magnetic rod sleeve clamping piece (7) is arranged on the lower frame (6), a magnetic rod (9) is arranged on the upper frame (8), a movable support (10) is arranged in the instrument main body (1) in a lifting manner, a cleaning sleeve (11) is arranged on the movable support (10), a reset spring (12) is fixedly connected on the movable support (10), a pressure receiving plate (13) is arranged on the movable support, an additional box (14) is fixedly connected on the instrument main body (1), the pressure receiving plate (13) stretches into the additional box (14), a movable piece (15) is movably arranged on the base (2), a movable support (16) is fixedly connected with a gear ring (16), a driving shaft (21) is arranged in the additional box (14), a driving structure is arranged on the gear ring, a driving shaft (21) is connected with a driving structure, a driving shaft (23), be provided with the antiseptic solution storage structure of pressing out water on additional case (14), and antiseptic solution storage structure is connected with shower nozzle (27), be provided with mounting panel (29) in additional case (14), and install rope loop (30) on mounting panel (29) rotation, rope loop (30) are connected with transmission shaft (21) through one-way bearing (31), and fixedly connected with stay cord (32) on rope loop (30), have clamp plate (34) through guide bar (33) movable mounting on mounting panel (29), and stay cord (32) fixed connection is on clamp plate (34), clamp plate (34) press the top surface at clamp plate (13).
2. The nucleic acid extractor according to claim 1, wherein: the base (2) is arranged at the bottom of the cavity of the instrument main body (1), two deep hole plate grooves are formed in the limiting frame (3), the spring pins (4) are located on two sides of the deep hole plate grooves, and the lifting rail (5) is arranged on the side wall of the cavity of the instrument main body (1).
3. The nucleic acid extractor according to claim 1, wherein: the utility model discloses a magnetic instrument, including lower frame (6), upper frame (8), bar magnet cover fastener (7), movable support (10) are located bar magnet (9), and clean cover (11) cup joint on bar magnet (9), reset spring (12) are connected at the top of instrument main part (1) cavity, and lower frame (6) and upper frame (8) parallel arrangement, and bar magnet cover fastener (7) set up in the bottom of lower frame (6), the bottom of upper frame (8) is arranged vertically to bar magnet (9), and movable support (10) are located bar magnet (9).
4. The nucleic acid extractor according to claim 1, wherein: the base (2) is internally provided with a cavity, the movable piece (15) is linearly and slidably arranged in the cavity in a limiting mode, the movable piece (15) is arranged on the surface of the base (2) in a protruding mode, the rack (16) is horizontally arranged, and the rack (16) extends into the additional box (14) from the base (2).
5. The nucleic acid extractor according to claim 1, wherein: the gear ring structure comprises a gear ring (17) which is arranged at the bottom of the additional box (14) in a limiting rotation mode, a spiral spring (18) is fixedly connected to the bottom of the gear ring (17), a push plate (19) is rotatably arranged on the inner wall of the gear ring (17), a stop block (20) is fixedly arranged on the inner wall of the gear ring (17), and an outer toothed plate (22) is annularly arranged on the transmission shaft (21).
6. The nucleic acid extractor according to claim 5, wherein: the gear ring (17) and the transmission shaft (21) are concentrically arranged, one end of the spiral spring (18) is connected with the gear ring (17), the other end of the spiral spring is connected with the inner wall of the additional box (14), the push plate (19) is connected with the outer gear plate (22), the stop block (20) is in contact connection with one side of the push plate (19), and the gear ring (17) is meshed with the rack (16).
7. The nucleic acid extractor according to claim 1, wherein: the disinfectant storage structure comprises a disinfectant tank (24) arranged on the additional tank (14), a liquid outlet tank (25) is arranged on the disinfectant tank (24), a pipeline (26) is connected to the liquid outlet tank (25), the pipeline (26) is communicated with a spray head (27), and a pressing pump (28) is arranged on the liquid outlet tank (25).
8. The nucleic acid extractor according to claim 7, wherein: the spray head (27) is arranged on the inner wall of the cavity of the instrument body (1), and the knocking plate (23) is positioned on the side face of the pressing pump (28).
9. The nucleic acid extractor according to claim 1, wherein: the mounting plate (29) is located between the additional box (14) and the instrument main body (1), the rope loop (30) is mounted at the bottom of the mounting plate (29) in a limiting rotation mode, the guide rod (33) is vertically arranged on the top surface of the mounting plate (29), and the pull rope (32) penetrates through the mounting plate (29) to be connected to the bottom surface of the pressing plate (34).
CN202310445984.3A 2023-04-24 2023-04-24 Magnetic bead method nucleic acid extraction instrument Active CN116254179B (en)

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