CN115252502A

CN115252502A - Preparation method of hibiscus mutabilis extract liposome and liposome thereof

Info

Publication number: CN115252502A
Application number: CN202211037722.5A
Authority: CN
Inventors: 王希丽; 张亚芬; 黄陈旸; 萧自智; 高媛媛; 刘浩; 欧高珍; 周贵和
Original assignee: Juwenlee Fujian Cosmetics Co ltd
Current assignee: Juwenlee Fujian Cosmetics Co ltd
Priority date: 2022-08-26
Filing date: 2022-08-26
Publication date: 2022-11-01
Anticipated expiration: 2042-08-26
Also published as: CN115252502B

Abstract

The present disclosure relates to a preparation method of liposome of Hibiscus mutabilis extract and the liposome thereof, which comprises the following steps: mixing and heating to dissolve 0.05-15% of the Hibiscus makinoi extract and membrane material with 10-50% of solvent by mass to obtain a lipid phase, wherein the mass ratio of the Hibiscus makinoi extract to the membrane material is 1: (0.2-5.5); mixing 1-6% by mass of pentanediol, 0.1-0.4% by mass of octyl glycol, 0.05-0.5% by mass of thickening agent and the balance of water, heating and dissolving to obtain a water phase; and mixing the lipid phase and the water phase, and homogenizing in a homogenizer to obtain the hibiscus mutabilis extract liposome. The method is simple and convenient to operate, the hibiscus extract liposome is prepared by wrapping the hibiscus extract by adopting a mechanical dispersion method, the water solubility of the hibiscus extract is improved, the antioxidant activity of the hibiscus extract is kept, and the hibiscus extract liposome has the characteristics of safety, slow release, long acting and the like; in addition, the used raw materials are low in cost and easy to produce on a large scale, and the practicability of the hibiscus mutabilis extract in cosmetics is improved.

Description

Preparation method of hibiscus mutabilis extract liposome and liposome thereof

Technical Field

The disclosure relates to the technical field of cosmetics, in particular to a preparation method of hibiscus mutabilis extract liposome and the liposome thereof.

Background

Plant extracts have a wide range of applications in cosmetics, often as skin conditioners, skin protectants, antioxidants, etc. 8972 kinds of cosmetic raw materials are used in China, and more than 3000 kinds of plant extracts exist in the 8972 kinds of cosmetic raw materials. In the cosmetic market, plant components are mostly added into cosmetics such as water, essence, cream and the like.

Hibiscus makinoi, also known as dog-head Hibiscus makinoi, qianbei beauty and demon flower, is produced in the tropical region of Asia, in the southwest of China, hainan, guangxi, etc. Patent CN105535115B shows that the active ingredients of Hibiscus makinoi mainly comprise lignans, polyphenols, phenylpropanoids, terpenoids and the like, and the compounds have the effects of resisting inflammation, oxidation, cancer and virus, improving immunity and the like. However, the solubility of the compounds in water is very low, most of the components can only be dissolved in a high-concentration organic reagent, and the water solubility is poor, so that the efficacy of the compounds is difficult to effectively exert; high concentrations of organic agents can easily cause skin irritation and even skin damage. In the field of cosmetics, the hibiscus makinoi extract is usually prepared into products such as cream emulsion and the like by adding an emulsifier, but the use of the emulsifier can also increase the risk of cytotoxicity and cause certain damage to human skin barriers. Therefore, the property of Hibiscus makinoi extract that is difficult to dissolve in water greatly limits its application in cosmetics.

The above is only for the purpose of assisting understanding of the technical solution of the present invention, and does not represent an admission that the above is the prior art.

Disclosure of Invention

In order to overcome the problems in the related art, the present disclosure provides a method for preparing liposome of hibiscus mutabilis extract.

According to a first aspect of the embodiments of the present disclosure, there is provided a method for preparing liposome of hibiscus mutabilis extract, comprising the following steps: mixing and heating to dissolve 0.05-15% of the Hibiscus makinoi extract and membrane material and 10-50% of solvent by mass to obtain a lipid phase, wherein the mass ratio of the Hibiscus makinoi extract to the membrane material is 1: (0.2-5.5%); mixing 1-6% by mass of pentanediol, 0.1-0.4% by mass of octaethylene glycol, 0.05-0.5% by mass of thickener and the balance of water, heating and dissolving to obtain a water phase; and mixing the lipid phase and the water phase, and homogenizing in a homogenizer to obtain the hibiscus mutabilis extract liposome.

In one embodiment, the homogenizer comprises an atmospheric homogenizer and a high pressure homogenizer; the rotation speed of the normal pressure homogenizer is 10000-14000rpm; the pressure value of the high-pressure homogenizer is not lower than 90MPa; mixing the lipid phase and the water phase, homogenizing in the normal pressure homogenizer for 1-5min, and homogenizing in the high pressure homogenizer for 10-20min to obtain Hibiscus Mutabilis extract liposome.

In one embodiment, the extraction site of the hibiscus makinoi extract comprises one or more of roots, stems, leaves, flowers and fruits of hibiscus makinoi.

In one embodiment, the extract part of hibiscus mutabilis is the root and/or stem of hibiscus mutabilis.

In one embodiment, the membrane material comprises phospholipid or phospholipid and cholesterol, and the mass fraction of the cholesterol is 0-1%; the phospholipid comprises one or more of hydrogenated lecithin, egg yolk lecithin, soybean lecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylethanolamine-polyethylene glycol 2000.

In one embodiment, the solvent comprises one of butanediol, ethanol.

In one embodiment, the thickener comprises one or more of polyacrylate crosspolymer-6, xanthan gum, ammonium acryloyldimethyltaurate/VP copolymer.

In one embodiment, 0.05-15% of the hibiscus sabdariffa extract, the hydrogenated lecithin and 10-50% of butanediol are mixed and heated to be dissolved to obtain a lipid phase, wherein the mass ratio of the hibiscus sabdariffa extract to the hydrogenated lecithin is 1:2; mixing 1-6% by mass of pentanediol, 0.1-0.4% by mass of octaethylene glycol, 0.1-0.5% by mass of polyacrylate cross-linked polymer-6 and the balance of water, heating and dissolving to obtain a water phase; mixing the lipid phase and the water phase, homogenizing in a normal pressure homogenizer with a rotation speed of 12000rpm for 3min, and homogenizing in a high pressure homogenizer with a pressure value of 90MPa for 10-20min to obtain Hibiscus Mutabilis extract liposome.

In one embodiment, 2% by mass of the hibiscus makinoi extract, the hydrogenated lecithin and 20% by mass of butanediol are mixed and heated to be dissolved, so as to obtain a lipid phase, wherein the mass ratio of the hibiscus makinoi extract to the hydrogenated lecithin is 1:2; mixing pentanediol accounting for 4% of mass fraction, octyl glycol accounting for 0.2% of mass fraction, polyacrylate cross-linked polymer-6 accounting for 0.2% of mass fraction and the balance of water, heating and dissolving to obtain a water phase; mixing the lipid phase and the water phase, homogenizing in a normal pressure homogenizer with a rotation speed of 12000rpm for 3min, and homogenizing in a high pressure homogenizer with a pressure value of 90MPa for 10-20min to obtain Hibiscus Mutabilis extract liposome.

According to a second aspect of the embodiments of the present disclosure, there is provided a liposome of hibiscus makinoi extract, including: the liposome is prepared by the preparation method of the hibiscus mutabilis extract liposome provided by the embodiment of the disclosure.

The technical scheme provided by the embodiment of the disclosure can have the following beneficial effects:

the preparation method of the hibiscus-manihot-extract liposome provided by the embodiment of the disclosure has a simple preparation process, and the hibiscus-manihot-extract is wrapped by a mechanical dispersion method, so that the water solubility of the hibiscus-manihot-extract is improved, the antioxidant activity of the hibiscus-manihot-extract is kept, and the hibiscus-manihot-extract liposome has the characteristics of safety, slow release, long acting and the like. The hibiscus sabdariffa extract liposome is prepared from the hibiscus sabdariffa extract by a mechanical dispersion method, so that the water solubility of the hibiscus sabdariffa extract is effectively improved. In-vitro experiments show that the hibiscus sabdariffa extract liposome has uniform particle size, high safety and strong antioxidant activity, and can be used as a raw material to be added into cosmetics. The method is simple and convenient to operate, the used raw materials are low in cost, large-scale production is easy to realize, and the method has a large application potential.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the disclosure.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present disclosure and together with the description, serve to explain the principles of the disclosure.

FIG. 1 shows a method for preparing liposomes from Hibiscus mutabilis extract according to a first embodiment.

Fig. 2 shows a method for preparing liposome of Hibiscus mutabilis extract according to a second embodiment.

Fig. 3 shows a method for preparing liposome of Hibiscus mutabilis extract according to a third embodiment.

FIG. 4 shows a fourth embodiment A method for preparing liposome of Hibiscus Mutabilis extract.

Fig. 5 shows a method for preparing liposome of Hibiscus mutabilis extract according to the fifth embodiment.

Fig. 6 shows a method for preparing liposome of Hibiscus mutabilis extract according to the sixth embodiment.

Fig. 7 shows a method for preparing liposome of Hibiscus mutabilis extract according to the seventh embodiment.

Fig. 8 shows a method for preparing liposome of Hibiscus mutabilis extract according to the eighth embodiment.

Fig. 9 shows a ninth embodiment of a method for preparing liposome of Hibiscus mutabilis extract.

Fig. 10 shows a method for preparing liposome of Hibiscus mutabilis extract according to the tenth embodiment.

FIG. 11 is a comparison of the appearance of liposomes of Hibiscus makinoi extracts prepared according to example 4-1, example 6-1 and example 10-1.

FIG. 12 is a morphological diagram of liposomes of Hibiscus makinoi extract prepared according to example 6-2.

FIG. 13 is a graph showing the results of the encapsulation efficiency measurements of the liposomes of Hibiscus makinoi extracts prepared according to examples 1-2, 2-2, 4-2, 6-2, 8-2 and 10-2.

Fig. 14 is a schematic diagram of the results of particle size detection of the liposome of hibiscus extract prepared according to the method for preparing liposome of hibiscus extract provided in example 6-2.

Fig. 15 is a schematic diagram showing the results of measuring the solubility of liposome of hibiscus extract in water, which is prepared according to the method for preparing liposome of hibiscus extract provided in example 6-2.

Fig. 16 is a schematic diagram of the detection result of the effect of the hibiscus extract liposome on NO release, which is prepared according to the preparation method of hibiscus extract liposome provided in example 6-2.

Detailed Description

Reference will now be made in detail to the exemplary embodiments, examples of which are illustrated in the accompanying drawings. When the following description refers to the accompanying drawings, like numbers in different drawings represent the same or similar elements unless otherwise indicated. The implementations described in the exemplary embodiments below are not intended to represent all implementations consistent with the present disclosure. Rather, they are merely examples of apparatus and methods consistent with certain aspects of the present disclosure, as detailed in the appended claims.

In order to solve the related technical problems, the embodiments of the present disclosure provide a method for preparing a liposome of hibiscus mutabilis extract and a liposome of hibiscus mutabilis extract. For convenience of explanation, the ratio of hibiscus sabdariffa extract to phospholipids is now named hibiscus sabdariffa extract liposome.

Examples

Extracting: refers to a solution obtained by soaking or mixing a substance to be extracted in a solvent. The extraction process can be carried out with the aid of the selection of suitable solvents, such as: water, ethanol/water mixture, methanol, propanol, butanol, chloroform, acetone or other organic solvent, and extracting by separation, percolation, decoction, countercurrent extraction, ultrasonic extraction, microwave extraction, or supercritical fluid extraction to obtain extract, and further evaporating and concentrating the extract to obtain extract and dried extract. In the disclosed embodiment, the extraction site of the hibiscus mutabilis extract comprises one or more of roots, stems, leaves, flowers and fruits of hibiscus mutabilis, and preferably roots and/or stems of hibiscus mutabilis. The extraction process is preferably carried out in an ethanol solvent, and ethanol with the mass fraction of 80% is preferably used for extraction.

The membrane material comprises phospholipid or phospholipid and cholesterol, and the mass fraction of the cholesterol is 0-1%; the phospholipid comprises one or more of hydrogenated lecithin, egg yolk lecithin, soybean lecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylethanolamine-polyethylene glycol 2000. The membrane material is preferably hydrogenated lecithin.

The solvent comprises one of butanediol and ethanol, and the solvent is preferably butanediol.

The thickener comprises one or more of polyacrylate cross-linked polymer-6, xanthan gum and acryloyl dimethyl ammonium taurate/VP copolymer, and preferably polyacrylate cross-linked polymer-6.

In the tables shown in FIGS. 1-10, A represents Hibiscus mutabilis extract; b represents membrane material, B1 represents phospholipid, B2 represents cholesterol, B11 represents hydrogenated lecithin, B12 represents egg yolk lecithin, B13 represents soybean lecithin, B14 represents phosphatidylcholine, B15 represents phosphatidylethanolamine, B16 represents phosphatidylglycerol, and B17 represents phosphatidylethanolamine-polyethylene glycol 2000; c represents a solvent, C1 represents butanediol, and C2 represents ethanol; d represents pentanediol; e represents Xin Gansuan; f represents a thickener, F1 represents polyacrylate cross-linked polymer-6, F2 represents xanthan gum, and F3 represents ammonium acryloyldimethyltaurate/VP copolymer.

Example 1

Preparation 1:0.2 Hibiscus manihot extract liposome, the specific embodiment is shown in figure 1:

examples 1 to 1

Preparation 1:0.2 preparation method of Hibiscus manihot extract liposome comprises the following steps: mixing and heating to dissolve 0.05% of the hibiscus makinoi extract, 0.01% of hydrogenated lecithin and 10% of butanediol to obtain a lipid phase, wherein the mass ratio of the hibiscus makinoi extract to the hydrogenated lecithin is 1:0.2. mixing 1% by mass of pentanediol, 0.1% by mass of octaethylene glycol, 0.05% by mass of xanthan gum and the balance of water, and heating to dissolve to obtain a water phase. Mixing the lipid phase and the water phase, and homogenizing in a normal pressure homogenizer with the rotation speed of 10000rpm for 5min to obtain a mixture of 1:0.2 Liposome of Hibiscus Mutabilis extract.

Examples 1 to 2

Preparation 1:0.2 preparation method of Hibiscus manihot extract liposome comprises the following steps: the preparation of lipid phase and water phase and normal pressure homogenization steps are the same as those in example 1-1, and then the mixture is homogenized by a high pressure homogenizer with a pressure value of 90MPa for 10-20min to obtain 1:0.2 Liposome of Hibiscus Mutabilis extract.

Examples 1 to 3

Preparation 1:0.2 preparation method of Hibiscus manihot extract liposome comprises the following steps: the lipid phase component contained 0.25% more cholesterol by mass than in example 1-1, and the rest of the procedure was the same as in example 1-1.

The results of comparing the performance status of the liposome of Hibiscus mutabilis extract obtained in example 1-1 with that obtained in example 1-2 are as follows:

1 prepared in examples 1-2: 0.2 liposomes of Hibiscus mutabilis extract to 1: the 0.2 Hibiscus mutabilis extract liposome is fine and smooth, has a smooth surface, relatively proper viscosity, less bubbles and relatively good appearance.

Comparing the example 1-1 with the example 1-3, the results of the comparison of the performance states of the hibiscus masufficiently extract liposome are as follows:

1 prepared in example 1-1: 0.2 liposomes of Hibiscus mutabilis extract to 1:0.2 the liposome of Hibiscus mutabilis extract has relatively good water solubility, relatively large particle size and relatively poor stability.

Example 2

Preparation 1:0.6 Liposome of Hibiscus Mutabilis extract, and its specific embodiment is shown in FIG. 2.

Example 3

Preparation 1: fig. 3 shows an embodiment of liposome of Hibiscus makinoi extract.

Example 4

Preparation 1: fig. 4 shows an embodiment of liposome of Hibiscus makinoi extract.

Example 5

Preparation 1: fig. 5 shows an embodiment of liposome of Hibiscus makinoi extract.

Example 6

Preparation 1: fig. 6 shows an embodiment of liposome of Hibiscus makinoi extract.

Example 7

Preparation 1: fig. 7 shows an embodiment of liposome of Hibiscus makinoi extract.

Example 8

Preparation 1:3.5 Liposome of Hibiscus Mutabilis extract, and its specific embodiment is shown in FIG. 8.

Example 9

Preparation 1:3.5 Liposome of Hibiscus Mutabilis extract, and its specific embodiment is shown in FIG. 9.

Example 10

Preparation 1:5 Hibiscus manihot Linn extract liposome, the concrete example is shown in figure 10.

FIG. 11 is a comparison of the appearance of liposomes of Hibiscus makinoi extracts prepared according to example 4-1, example 6-1 and example 10-1. FIG. 12 is a representation of liposomes of Hibiscus makinoi extract prepared according to example 6-2.

As shown in fig. 11, in the examples of the present disclosure, when comparing the expressions of the liposome of hibiscus extract prepared in examples 4-1 and 6-1 with those of example 10-1, the viscosity of hibiscus extract is relatively increased, the fluidity of hibiscus extract is relatively poor, and the surface unevenness of hibiscus extract is relatively increased as the mass ratio of hibiscus extract to phospholipid is increased. In addition, the liposome of Hibiscus makinoi extract prepared in example 4-1 has significantly more air bubbles than the liposomes of Hibiscus makinoi extract prepared in examples 6-1 and 10-1.

As shown in fig. 11 and 12, in the embodiment of the present disclosure, by comparing the appearance of the hibiscus extract liposome prepared in example 6-1 with that of example 6-2, the hibiscus extract liposome obtained by two-step homogenization in the normal pressure homogenizer and the high pressure homogenizer is more fine, relatively smooth in surface, suitable in viscosity, less in bubbles, and better in appearance.

In the embodiment of the disclosure, the liposome of the hibiscus extract prepared by the preparation method of the liposome of the hibiscus extract provided by the embodiment of the disclosure is subjected to the following entrapment rate detection, the hibiscus extract has poor water solubility, the free hibiscus extract is obtained by adopting a low-speed centrifugation method, and the entrapment rate is calculated by detecting the total polyphenol content in the free hibiscus extract and the hibiscus extract of the liposome thereof. The specific process is as follows:

taking 0.1g of 2 parts of hibiscus sabdariffa extract liposome, adding 2mL of 60% ethanol aqueous solution into 1 part of the hibiscus sabdariffa extract liposome, adding 2mL of water into the other 1 part of hibiscus sabdariffa extract liposome, fully mixing and dissolving, centrifuging the hibiscus sabdariffa extract liposome aqueous solution at 2000rpm for 10min, pouring out the supernatant hibiscus sabdariffa extract liposome aqueous solution to obtain a free hibiscus sabdariffa extract, re-suspending with 200 mu L of 60% ethanol aqueous solution, and detecting the total polyphenol content in the 2 parts of samples by adopting a forskolin phenol method. Envelope rate calculation formula: encapsulation ratio = (C2-C1 × 10)/C2 × 100%; in the formula: c1 is the total polyphenol concentration (mu g/mL) of the free hibiscus makinoi extract; c2 is total polyphenol concentration (mu g/mL) of the hibiscus sabdariffa extract in the liposome; 10 is the dilution multiple of the total polyphenol concentration of the free hibiscus makinoi extract. The calculation results are shown in fig. 13: it is known that the mass ratio of the hibiscus mutabilis extract to the hydrogenated lecithin is 1:2, the encapsulation efficiency is highest, and the encapsulation effect can be optimized by using a high-pressure homogenizer.

Now, taking example 6-2 as an example, the following reaction mixture was prepared for example 6-2, 1:2, carrying out the following detection on the hibiscus mutabilis extract liposome:

for 1:2, detecting the particle size of the hibiscus mutabilis extract liposome: the particle size of 1: the particle size of the hibiscus sabdariffa extract liposome is shown in fig. 14. Example 6-2 preparation of 1: the grain diameter range of the 2 Hibiscus mutabilis extract liposome is 0.5-2.0 μm, the area average diameter is 1.066 μm, and the volume average diameter is 1.336 μm.

Under the same conditions, the reaction was carried out by reacting 1:2 liposomes of Hibiscus makinoi extract were compared to 1:2 comparison of entrapment rates of Hibiscus majaeither extract liposomes shows that, in example 6-2, compared with example 6-9, liposomes prepared from hydrogenated lecithin had a uniform particle size and a better entrapment rate than liposomes prepared from soybean lecithin. Example 6-2 compared to examples 6-11, liposomes prepared with hydrogenated lecithin had higher saturation, no off-flavor, and better stability than liposomes prepared with egg yolk lecithin.

For 1:2, testing the solubility of the hibiscus mutabilis extract liposome in water: dissolving 0.2g of Hibiscus makinoi extract in 8g of water to prepare an aqueous solution of the Hibiscus makinoi extract with the mass fraction of 2%. Precisely weighing 0.01g of Hibiscus makinoi extract, dissolving in 9.99g of water, and preparing into an aqueous solution of Hibiscus makinoi extract with mass fraction of 0.1%. The mixture prepared in example 6-2 was taken as 1:2 dissolving 0.2g of hibiscus manihot extract liposome (the proportion of hibiscus manihot extract is 2%) in 8g of water to prepare an aqueous solution of hibiscus manihot extract liposome with the final mass fraction concentration of 0.1%. As shown in fig. 15, it was found that the hibiscus mutabilis extract was partially dissolved in water, but the residue was not dissolved, and was not dissolved even when the volume of water was increased. After the hibiscus makinoi extract is prepared into the liposome, the hibiscus makinoi extract can be uniformly dispersed in water and is a uniform yellow aqueous solution, and the problem that active ingredients in the hibiscus makinoi extract are difficult to dissolve in water is effectively solved.

For 1:2, detecting the oxidation resistance of the hibiscus mutabilis extract liposome: the method for detecting the ratio of the blank liposome (without the hibiscus makinoi extract, the rest steps are the same as the example 6-2) and the 1:2 antioxidant activity of the hibiscus sabdariffa extract liposome, expressed as DPPH clearance, and the detection results are shown in the following table.

Group of	DPPH clearance (%)
		Blank liposomes	15.56
1 prepared in example 6-2: 2 Hibiscus manihot extract liposome	60.49

It can be seen that, compared with the blank liposome, 1:2, the antioxidant activity of the hibiscus sabdariffa extract liposome is remarkably improved, which shows that the liposome structure effectively retains the antioxidant activity of the hibiscus sabdariffa extract.

For 1:2 detection of effect of hibiscus mutabilis extract liposome on NO release: the mixture of 1 prepared in example 6-2: 2 dissolving the liposome of the hibiscus sabdariffa extract by using sterile normal saline (the final concentration of the mass fraction of the hibiscus sabdariffa extract is 0.1 percent), and diluting the blank liposome by the same dilution times as 1:2, dissolving the hibiscus sabdariffa extract in DMSO, and diluting with PBS. First, blank liposomes, hibiscus mutabilis extract and 1: influence of 2 Hibiscus makinoi extract liposome on RAW264.7 cell viability.

The cells were collected, and an appropriate sample concentration was selected based on the result of CCK-8, and LPS was added to a final concentration of 1. Mu.g/mL. And after the sample and the LPS are incubated for 24h, collecting cell supernatant, and detecting the content of NO by using an NO detection kit. The detection result is shown in fig. 16, compared with the blank group, after LPS acts on RAW264.7 cells, the cell release NO is obviously increased, which indicates that LPS can effectively stimulate macrophage inflammatory reaction. The blank liposome is not obviously different from an LPS group, the mountain lotus extract and the liposome prepared in the example 6-2 can obviously inhibit LPS-induced NO release of RAW264.7 cells, and meanwhile, the inhibition activity of the mountain lotus extract is higher than that of the liposome, which shows that the liposome structure can play a role in slow release and long acting.

In conclusion, the preparation method of the hibiscus mutabilis extract liposome provided by the embodiment of the disclosure is simple and convenient to operate, low in cost of used raw materials, easy for large-scale production, capable of improving the water solubility of the hibiscus mutabilis extract, and capable of keeping the antioxidant activity of the hibiscus mutabilis extract, and has great application potential. The hibiscus sabdariffa extract liposome prepared by the preparation method provided by the embodiment of the disclosure has the advantages of uniform particle size, high safety, slow release and long-acting effect, can be used as a raw material to be added into cosmetics, has great application potential, and is worthy of popularization.

It is understood that "a plurality" in this disclosure means two or more, and other words are analogous. "and/or" describes the association relationship of the associated object, indicating that there may be three relationships, for example, a and/or B, which may indicate: a exists alone, A and B exist simultaneously, and B exists alone. The character "/" generally indicates that the former and latter associated objects are in an "or" relationship. The singular forms "a", "an", and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise.

It will be further understood that the terms "first," "second," and the like are used to describe various information and that such information should not be limited by these terms. These terms are only used to distinguish one type of information from another and do not denote a particular order or importance. Indeed, the terms "first," "second," and the like are fully interchangeable. For example, first information may also be referred to as second information, and similarly, second information may also be referred to as first information, without departing from the scope of the present disclosure.

It will be further understood that the terms "central," "longitudinal," "lateral," "front," "rear," "upper," "lower," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in an orientation or positional relationship indicated in the drawings for convenience in describing the present embodiment and to simplify the description, but do not indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation.

It will be further understood that, unless otherwise specified, "connected" includes direct connections between the two without the presence of other elements, as well as indirect connections between the two with the presence of other elements.

It is further to be understood that while operations are depicted in the drawings in a particular order, this is not to be understood as requiring that such operations be performed in the particular order shown or in serial order, or that all illustrated operations be performed, to achieve desirable results. In certain environments, multitasking and parallel processing may be advantageous.

Other embodiments of the disclosure will be apparent to those skilled in the art from consideration of the specification and practice of the disclosure disclosed herein. This application is intended to cover any variations, uses, or adaptations of the disclosure following, in general, the principles of the disclosure and including such departures from the present disclosure as come within known or customary practice within the art to which the disclosure pertains. It is intended that the specification and examples be considered as exemplary only, with a true scope and spirit of the disclosure being indicated by the following claims.

It will be understood that the present disclosure is not limited to the precise arrangements described above and shown in the drawings and that various modifications and changes may be made without departing from the scope thereof. The scope of the present disclosure is limited only by the appended claims.

Claims

1. The preparation method of the hibiscus mutabilis extract liposome is characterized by comprising the following steps:

mixing and heating to dissolve 0.05-15% of the Hibiscus makinoi extract and membrane material and 10-50% of solvent by mass to obtain a lipid phase, wherein the mass ratio of the Hibiscus makinoi extract to the membrane material is 1: (0.2-5.5%);

mixing 1-6% by mass of pentanediol, 0.1-0.4% by mass of octaethylene glycol, 0.05-0.5% by mass of thickener and the balance of water, heating and dissolving to obtain a water phase;

and mixing the lipid phase and the water phase, and homogenizing in a homogenizer to obtain the hibiscus mutabilis extract liposome.

2. The method for preparing the liposome of Hibiscus mutabilis extract according to claim 1, wherein the liposome of Hibiscus mutabilis extract comprises:

the homogenizer comprises a normal pressure homogenizer and a high pressure homogenizer;

the rotation speed of the normal pressure homogenizer is 10000-14000rpm;

the pressure value of the high-pressure homogenizer is not lower than 90MPa;

mixing the lipid phase and the water phase, homogenizing in the normal pressure homogenizer for 1-5min, and homogenizing in the high pressure homogenizer for 10-20min to obtain Hibiscus Mutabilis extract liposome.

3. The method for preparing the liposome of Hibiscus mutabilis extract according to claim 1, wherein the liposome of Hibiscus mutabilis extract comprises:

the extract part of Hibiscus makinoi extract comprises one or more of root, stem, leaf, flower and fruit of Hibiscus makinoi.

4. The method for preparing the hibiscus sabdariffa extract liposome according to claim 3, wherein the liposome is prepared from the following components:

the extraction part of the Hibiscus makinoi extract is the root and/or stem of Hibiscus makinoi.

5. The method for preparing the liposome of Hibiscus mutabilis extract according to claim 1, wherein the liposome of Hibiscus mutabilis extract comprises:

the membrane material comprises phospholipid or phospholipid and cholesterol, and the mass fraction of the cholesterol is 0-1%;

the phospholipid comprises one or more of hydrogenated lecithin, egg yolk lecithin, soybean lecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylethanolamine-polyethylene glycol 2000.

6. The method for preparing the hibiscus sabdariffa extract liposome according to claim 1, wherein the hibiscus sabdariffa extract liposome is prepared from the following raw materials:

the solvent comprises one of butanediol and ethanol.

7. The method for preparing the liposome of Hibiscus mutabilis extract according to claim 1, wherein the liposome of Hibiscus mutabilis extract comprises:

the thickening agent comprises one or more of polyacrylate cross-linked polymer-6, xanthan gum and acryloyl dimethyl ammonium taurate/VP copolymer.

8. The method for preparing the hibiscus amurensis extract liposome according to claim 1, wherein the method comprises the following steps:

mixing and heating to dissolve 0.05-15% of the Hibiscus makinoi extract, hydrogenated lecithin and 10-50% of butanediol by mass fraction to obtain a lipid phase, wherein the mass ratio of the Hibiscus makinoi extract to the hydrogenated lecithin is 1:2;

mixing 1-6% by mass of pentanediol, 0.1-0.4% by mass of octaethylene glycol, 0.1-0.5% by mass of polyacrylate cross-linked polymer-6 and the balance of water, heating and dissolving to obtain a water phase;

mixing the lipid phase and the water phase, homogenizing in a normal pressure homogenizer at 12000rpm for 3min, and homogenizing in a high pressure homogenizer at a pressure value of 90MPa for 10-20min to obtain Hibiscus Mutabilis extract liposome.

9. The method for preparing the liposome of Hibiscus mutabilis extract according to claim 8, wherein the liposome of Hibiscus mutabilis extract comprises:

mixing and heating to dissolve 2% of the Hibiscus makinoi extract, hydrogenated lecithin and 20% of butanediol by mass fraction to obtain a lipid phase, wherein the mass ratio of the Hibiscus makinoi extract to the hydrogenated lecithin is 1:2;

mixing 4 mass percent of pentanediol, 0.2 mass percent of octyl glycol and 0.2 mass percent of polyacrylate cross-linked polymer-6 with the balance of water, heating and dissolving to obtain a water phase;

mixing the lipid phase and the water phase, homogenizing in a normal pressure homogenizer with a rotation speed of 12000rpm for 3min, and homogenizing in a high pressure homogenizer with a pressure value of 90MPa for 10-20min to obtain Hibiscus Mutabilis extract liposome.

10. The hibiscus mutabilis extract liposome is characterized by comprising the following components:

the liposome of Hibiscus mutabilis extract is prepared by the method of any one of claims 1 to 9.