CN115252472A

CN115252472A - Antimicrobial essential oil compositions, products containing the same and methods of making the same

Info

Publication number: CN115252472A
Application number: CN202210059971.8A
Authority: CN
Inventors: 贾振华
Original assignee: Shijiazhuang Yiling Pharmaceutical Co Ltd
Current assignee: Shijiazhuang Yiling Pharmaceutical Co Ltd
Priority date: 2021-04-30
Filing date: 2022-01-19
Publication date: 2022-11-01

Abstract

The invention discloses an antimicrobial essential oil composition, which comprises: carvacrol; thymol; one or more selected from lemon oil, sweet orange oil, citrus aurantium peel oil, lime oil and pomelo peel oil; one or more selected from the group consisting of peppermint oil, spearmint oil, peppermint oil and peppermint oil; one or more selected from laurel leaf oil and marjoram oil; one or more selected from patchouli oil, sandalwood oil and rosemary oil; one or more selected from geranium oil, pine needle oil and cedar wood oil; and optionally one or more selected from perilla oil, chrysanthemum morifolium oil and vitex negundo oil. The invention also discloses a disinfection product, a daily necessity and a food containing the antimicrobial essential oil composition, and a preparation method of the antimicrobial essential oil composition.

Description

Antimicrobial essential oil compositions, products containing the same, and methods of making the same

Technical Field

The present application relates generally to essential oil compositions, and more particularly to plant essential oil compositions having antimicrobial functionality. The application also relates to products comprising the antimicrobial essential oil composition, and methods of making the antimicrobial essential oil composition.

Background

Many highly pathogenic microbial pathogens cause rapidly-onset and epidemic infectious diseases. Such infectious diseases can spread even in a short time across provinces, countries, or all over the world, i.e., rapidly develop into "pandemics".

During a pandemic period, killing is an important work for epidemic prevention and control. At present, synthetic chemical-based disinfection products (such as ethanol, peracetic acid, chlorine-containing disinfectants, and the like), ultraviolet rays, heat, and the like are commonly used worldwide to inactivate pathogenic microorganisms. However, such disinfection measures have the risks of inconvenient application, limited use, or easy secondary harm and pollution to human bodies and the environment.

Essential oil products based on natural plants have traditionally been used for the treatment of infectious diseases and the control of epidemics for thousands of years. The antimicrobial activity of part of plant essential oils has been demonstrated by a number of studies. The plant essential oil product is harmless to human bodies and environment-friendly while inactivating pathogenic microorganisms, and accords with the trend of green, environment-friendly and sustainable development.

Thus, there is a need to provide plant essential oil products with antimicrobial activity.

We have surprisingly found that by using a combination of a plurality of single essential oils, in combination with specific antimicrobial active ingredients, a biocidal product can be obtained which has good antimicrobial properties and which is harmless to the human body and environmentally friendly.

Disclosure of Invention

To solve the above problems, in an embodiment of the present application, there is provided an antimicrobial essential oil composition, wherein components of the essential oil composition are derived from natural plants, and the combination of these components provides the resulting essential oil composition with good antimicrobial properties.

Embodiments of the present application provide antimicrobial essential oil compositions. The antimicrobial essential oil composition comprises, based on the total weight of the composition: (1) 0.001-80% of one or more selected from carvacrol and thymol; (2) 0.001-99.9% of one or more selected from lemon oil, sweet orange oil, bitter orange peel oil, lime oil and shaddock peel oil; (3) 0.001-99.9% of one or more selected from the group consisting of peppermint oil, spearmint oil, peppermint oil and peppermint oil; (4) 0.001-10% of one or more selected from laurel leaf oil and sweet origanum oil; (5) 0.001-5% of one or more selected from patchouli oil, sandalwood oil and rosemary oil; (6) 0.001-20% of one or more selected from geranium oil, pine needle oil and cedar wood oil; and (7) optionally, 0.001-20% of one or more selected from perilla oil, chrysanthemum morifolium oil and vitex leaf oil.

In another aspect, the antimicrobial essential oil composition comprises (1) 2-80% of one or more selected from carvacrol and thymol; (2) 2-50% of one or more selected from lemon oil, sweet orange oil, bitter orange peel oil, lime oil and shaddock peel oil; (3) 5-40% of one or more selected from peppermint oil, peppermint oil and peppermint oil; (4) 2-20% of one or more selected from laurel leaf oil and sweet origanum oil; (5) 0.1-5% of one or more selected from patchouli oil, sandalwood oil and rosemary oil; (6) 2-20% of one or more selected from geranium oil, pine needle oil and cedar wood oil; and (7) optionally, 2-15% of one or more selected from perilla oil, chrysanthemum morifolium oil and vitex leaf oil.

In one aspect, the antimicrobial essential oil composition further optionally comprises one or more selected from the group consisting of: (8) 0.0001-20% of honeysuckle oil; (9) 0.0001-20% of ligusticum oil; (10) 0.0001-20% of xanthium oil; and (11) 0.0001-20% of weeping forsythia oil.

In another aspect, the antimicrobial essential oil composition comprises: (1) 5% carvacrol and 5% thymol; (2) 35% of lemon oil; (3) 30% of an asian peppermint oil; (4) 10% bay leaf oil; (5) 0.5% of patchouli oil; (6) 5% geranium oil; and (7) 9.5% perilla oil.

In another aspect, the antimicrobial essential oil composition comprises: (1) 0.005% of carvacrol and 0.005% of thymol; (2) 0.1% lemon oil; (3) 99.58% peppermint oil asia; (4) 0.005% of bay leaf oil; (5) 0.25% of patchouli oil; (6) 0.005% geranium oil; and (7) 0.05% perilla oil.

In another aspect, the antimicrobial essential oil composition comprises: (1) 0.005% of carvacrol and 0.005% of thymol; (2) 99.5% lemon oil; (3) 0.25% peppermint oil asian; (4) 0.005% of bay leaf oil; (5) 0.18% of patchouli oil; (6) 0.005% geranium oil; and (7) 0.05% perilla oil.

In another aspect, the antimicrobial essential oil composition comprises: (1) 51.25% carvacrol; (2) 26.25% thymol; (3) 8.75% lemon oil; (4) 7.5% of Asian peppermint oil; (5) 2.5% of bay leaf oil; (6) 0.125% of patchouli oil; (7) 1.25% geranium oil; and (8) 2.375% perilla oil.

In yet another aspect, the antimicrobial essential oil composition comprises: (1) 25% carvacrol and 25% thymol; (2) 2% lemon oil; (3) 10% peppermint oil asia; (4) 20% of bay leaf oil; (5) 0.5% of patchouli oil; (6) 2% geranium oil; (7) 5% of honeysuckle flower oil; (9) 4% of ligusticum wallichii oil; (10) 0.3% of xanthium oil; and (11) 0.2% forsythia oil.

In yet another aspect, the microorganism is selected from the group consisting of bacteria, fungi, viruses, and combinations thereof.

In yet another aspect, the microorganism is a new coronavirus.

Embodiments of the present application also provide a product for reducing or killing microorganisms on one or more of a hard surface, a textile, skin, a mucous membrane, or hair, wherein the product comprises an antimicrobial essential oil composition as any one of described above, and optionally one or more adjuvants.

In one aspect, the product is one or more selected from the group consisting of a disinfecting product, a commodity, a protective product, and a food product. The commodity is one or more selected from the group consisting of cosmetics, home care products, and personal care products, and in another aspect, the commodity is one or more selected from the group consisting of cosmetics, home care products, and personal care products. The sterilizing product comprises one or more of a mask bead, an air freshener, an oral spray, a hand sanitizer, a surface cleaner and a detergent; the food is one or more of chewing gum, bead, gel candy, hard candy, soft candy, beverage, and dietary supplement. The daily necessities can also comprise one or more of a mask bead explosion mask, a civil mask and a medical mask.

Embodiments of the present application also provide a method of preparing the above antimicrobial essential oil composition, the method comprising: all ingredients of the antimicrobial essential oil composition were mixed homogeneously.

In one aspect, the mixing is conducted at ambient temperature and pressure.

The application method also provides a method for preparing the mask from the antimicrobial essential oil composition, which comprises the steps of preparing the essential oil composition into essential oil master batches or essential oil microcapsules, and then preparing the mask according to a conventional method.

Detailed Description

For purposes of the following detailed description, it is to be understood that the application may assume various alternative variations and step sequences, except where expressly specified to the contrary. Moreover, other than in any operating examples, or where otherwise indicated, all numbers expressing, for example, quantities of ingredients used in the specification and claims are to be understood as being modified in all instances by the term "about". Accordingly, unless indicated to the contrary, the numerical parameters set forth in the following specification and attached claims are approximations that may vary depending upon the desired properties to be obtained by the present application. At the very least, and not as an attempt to limit the application of the doctrine of equivalents to the scope of the claims, each numerical parameter should at least be construed in light of the number of reported significant digits and by applying ordinary rounding techniques.

Notwithstanding that the numerical ranges and parameters setting forth the broad scope of the application are approximations, the numerical values set forth in the specific examples are reported as precisely as possible. Any numerical value, however, inherently contains certain errors necessarily resulting from the standard deviation found in their respective testing measurements.

Moreover, it should be understood that any numerical range recited herein is intended to include all sub-ranges subsumed therein. For example, a range of "1 to 10" is intended to include all sub-ranges between (and including) the recited minimum value of 1 and the recited maximum value of 10, i.e., having a minimum value equal to or greater than 1 and a maximum value of equal to or less than 10.

In this application, the use of the singular includes the plural and plural encompasses singular, unless expressly stated otherwise. In addition, in this application, the use of "or" means "and/or" unless explicitly stated otherwise, even though "and/or" may be explicitly used in some cases. In addition, in this application, the use of "a" or "an" means "at least one" unless explicitly stated otherwise. For example, "a" polymer, "a" composition, and the like refer to one or more of any of these items.

In one embodiment of the present application, an antimicrobial essential oil composition is provided. As used herein, the term "antimicrobial" means that the product used has the activity of reducing/killing microorganisms. In the context of the present application, "microorganism" may include bacteria, fungi, viruses, mycoplasma, and the like, such as, but not limited to, coronaviruses (e.g., SARS-CoV (SARS virus), MERS-CoV (middle east respiratory syndrome virus), SARS-CoV-2 (neocoronavirus), and the like), hepatitis viruses (e.g., hepatitis a, b, c), influenza viruses (e.g., influenza a, b, and avian influenza viruses, and the like), staphylococcus aureus, pneumobacillus, typhoid, candida, molds, mycoplasma pneumoniae, and the like. In a preferred aspect, the virus in the context of the present application may be a new coronavirus.

As used herein, the term "plant essential oil" refers to the generic term for volatile aroma-containing substances obtained from the processing and extraction of natural aromatic plants. Generally, plant essential oils are volatile aromatic substances extracted from parts of plants such as flowers, leaves, roots, seeds, fruits, barks, resins, and hearts by steam distillation, cold pressing, fat absorption, or solvent extraction. Plant essential oils often have specific biological activities, and in particular, some plant essential oils have good antimicrobial activities, such as antibacterial, antifungal and/or antiviral activities, and can be used as effective environmentally friendly antimicrobial agents. The plant essential oil can be divided into single component essential oil and compound essential oil. Wherein, compared with single essential oil, the compound essential oil contains a plurality of effective essential oil components, and can realize significantly better biological activity, such as antimicrobial activity, through the synergistic effect of the components. Compound essential oils are preferably used in the present application.

In one aspect of the present application, an antimicrobial essential oil composition comprises:

(1) One or more selected from carvacrol and thymol, in an amount of 0.0001-99.99%, preferably 2-80%, more preferably 2-60%, and further preferably 6-16%;

(2) One or more selected from lemon oil, sweet orange oil, citrus aurantium peel oil, lime oil and grapefruit peel oil, in an amount of 0.0001 to 99.99%, preferably 2 to 80%, more preferably 2 to 50%, more preferably 10 to 50%, and still more preferably 32 to 38%;

(3) One or more selected from Mentha arvensis oil, mentha piperita oil, and Mentha piperita oil in an amount of 0.0001-99.99%, preferably 2-80%, more preferably 5-40%, further preferably 28-35%;

(4) One or more selected from laurel leaf oil and marjoram oil, in an amount of 0.0001-20%, preferably 2-20%, more preferably 2-18%, more preferably 2-15%, and further preferably 8-12%;

(5) One or more selected from patchouli oil, sandalwood oil and rosemary oil, in an amount of 0.0001-20%, preferably 0.1-18%, more preferably 0.1-5%, even more preferably 0.3-1%;

(6) One or more selected from geranium oil, pine needle oil and cedar wood oil, in an amount of 0.0001-20%, preferably 2-20%, more preferably 2-18%, even more preferably 3-8%,

the above percentages are based on the total weight of the composition.

In one aspect, the antiviral essential oil composition may further optionally comprise (7) one or more selected from perilla oil, chrysanthemum morifolium oil and vitex leaf oil in an amount of 0.0001 to 20%, preferably 2 to 18%, more preferably 2 to 15%, further preferably 8 to 12%, based on the total weight of the composition.

In another aspect, the antiviral essential oil composition may further optionally comprise at least one compound selected from (8) 0.0001-20% of honeysuckle oil; (9) 0.0001-20% of ligusticum wallichii oil; (10) 0.0001-20% of xanthium oil; and (11) 0.0001-20% of one or more of forsythia suspense oil, wherein the percentages are based on the total weight of the composition.

The various ingredients of the antimicrobial essential oil composition in the embodiments of the present application combine to achieve, through synergistic effects, superior antimicrobial properties. For example, when the essential oil composition of the present application is used for in vitro disinfection against new coronaviruses, the killing efficiency of the treatment for 3 and 30 minutes may be higher than 75%, e.g. higher than 80%, 85%, 90%, 95%, 98% or even close to 100%. Without wishing to be bound by any theory, applicants believe that the mechanism by which the essential oil composition kills new coronaviruses may be based, at least in part, on the following facts: the new coronavirus has an envelope structure, belongs to lipophilic (oleophylic) viruses, and is easy to adsorb and enrich by natural plant essential oil theoretically so as to be inactivated. The applicant researches and discovers that after the antiviral plant essential oil is contacted and mixed with the stock solution of the new coronavirus, the new coronavirus can be extracted from the stock solution, and then the new coronavirus can be inactivated quickly and efficiently. There is an active complementation and addition between the various essential oil components selected in this application, achieving unexpected synergy and significantly better antimicrobial properties than single essential oils.

In addition, other benefits may be realized by the essential oil compositions of the present application, including, but not limited to, partial components of the compositions that are capable of blocking the binding of neocoronaviruses to the ACE2 receptor of respiratory epithelial cells (i.e., achieving blocking/protection); some components can promote respiration and improve nasal obstruction caused by early viral upper respiratory tract infection or common cold (i.e. relieving effect). Moreover, the essential oil composition is prepared based on natural plant materials, has fragrant smell, and has the advantages of low irritation, low corrosivity, no toxicity or harm to human bodies, environmental friendliness and the like.

Also disclosed in another embodiment of the present application are products for the microbial disinfection of an environment or human, for example, for reducing or killing microorganisms on one or more of a hard surface, a textile, skin, mucous membrane, or hair. The product comprises the antimicrobial essential oil composition of the present application and optionally one or more adjuvants.

In the context of the present application, the term "hard surface" refers to environmental object surfaces other than textile surfaces and human body surfaces, including but not limited to ceilings, walls, floors/tiles, elevators, stairways, handrails, furniture, tableware, vehicles, etc. commonly used surfaces that are readily accessible in life; the term "textile" includes, but is not limited to, clothing, hats, towels, tablecloths, bedding, and the like; the term "skin" refers to the skin of a human or animal; the term "mucosal" refers to the oral, ocular or rectal mucosa of a human or animal; the term "hair" refers to human hair or body hair, or animal hair or feathers.

In one aspect, the products for microbial disinfection of the environment or human body described herein refer to products that are non-toxic, non-irritating, non-corrosive to the human body, environmentally friendly, and capable of reducing or killing harmful microorganisms in the environment or on the human body, including but not limited to disinfectant products, daily necessities, and foods. For example, the commodity may be a cosmetic, a home care product, and a personal care product, such as a mask, underwear, a pop-up, a tablecloth, and the like. Examples of products for microbial killing of the environment or human body may include, for example, but are not limited to, one or more of furniture cleaners, floor cleaners, kitchen cleaners, toilet cleaners, fabric detergents, disinfecting sprays, oral sprays, toothpastes, mouthwashes, body washes, shampoos, conditioners, skin care products, chewing gums.

In still further embodiments of the present application, there is also provided a method of preparing the antimicrobial essential oil composition as described above, comprising uniformly mixing all the ingredients of the antimicrobial essential oil composition. In one aspect, the mixing is conducted at ambient temperature and pressure.

The present application will be explained in further detail below with reference to examples. However, it will be understood by those skilled in the art that these examples are provided for illustrative purposes only and are not intended to limit the present application.

Examples

Embodiments of the present application will be described in detail below with reference to examples, but those skilled in the art will appreciate that the following examples are only illustrative of the present application and should not be construed as limiting the scope of the present application. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products commercially available. All amounts listed are described in parts by weight based on total weight unless otherwise indicated. This application is not to be construed as limited to the particular embodiments set forth herein.

Examples 1 to 8 and comparative examples 1 to 5

The components were mixed at normal temperature and pressure according to the formulations in tables 1-2 below to prepare the essential oil compositions of examples 1-13, wherein examples 1-5 were the essential oil compositions of the present application and examples 6-13 were the essential oil compositions of the comparative examples.

Test example 1

The essential oil composition of example 1 (labeled as food grade essential oil (FEO)) and the essential oil composition of example 3 (labeled as cosmetic grade essential oil (CEO)) were prepared separately.

Cytotoxicity of FEO and CEO and the effect of inactivating the New coronavirus (SARS-Cov-2) were tested according to the following test methods.

As can be seen from the above results, the essential oil compositions FEO and CEO of the present application have acceptable cytotoxicity and excellent killing effect of new coronavirus.

Test example 2

The essential oil compositions of the examples and comparative examples were evaluated at the cellular level for the effect against the novel coronavirus (SARS-CoV-2) according to the following manner.

Material

Cell: veroE6 cells.

Virus: SARS-CoV-2 (south African variant) with a titer of TCID₅₀ = 10^-5Per 100. Mu.L, using a viral titer of 100TCID₅₀。

Essential oil: the compound essential oil is diluted to 6 concentrations by a cell culture medium by adopting a multiple dilution method.

Method

Test for antiviral Properties of drug

(1) The tested drugs are:

(2) Sterile 96-well culture plate, 100. Mu.L of 1X 10 concentration per well⁵cells/mL VeroE6 cells, 5% CO at 37 ℃₂Culturing for 24 hours;

(3) Adding 100TCID into culture plate experimental group and virus control group₅₀Virus liquid 100 mul/well, 37 deg.C

5% CO₂Adsorbing for 2 hours by an incubator;

(4) After 2h, discarding the cell culture solution in the 96-hole culture plate; diluting the tested medicine into each concentration in table 1, wherein each concentration is 3 multiple holes, and adding the liquid medicine into each hole at 100 mu L;

(5) Simultaneously setting a cell control, a blank control (a solvent control), a virus control (a negative control) and a contrast medicine control;

(6) Cells at 37 ℃ 5% CO₂Incubating in an incubator for 3 days;

(7) Cytopathic effect (CPE) was observed under an optical microscope and the degree of cytopathic effect was recorded according to the following 6-point scale: "-" no lesions present; "±" means less than 10% cytopathic effect; "+" is about 25% of the cellular pathology; "+ +" indicates about 50% of cellular pathology; "+ + + +" is about 75% of the cells outThe existing pathological changes are as follows: "++++" indicates that more than 75% of the lesions are diseased. The half maximal effective concentration (IC) was calculated using the Reed-Muench method or GraphPad prism5.0₅₀). Judging the drug effect standard: concentrations that inhibit viral CPE by 50% are considered effective concentrations.

(8) The experimental conditions are as follows: the above experimental operations are all completed in a BSL-3 laboratory.

Results

1. MTS detection of compound essential oil cytotoxicity:

when the growth state of VeroE6 cells is the best, the cells are digested with 0.02% EDTA and 0.5% trypsin, the cells are adjusted to the required concentration with the growth medium, the cell medium is added into a 96-well cell culture plate at 100. Mu.L/well, and the cells are cultured in a 5% CO2 incubator at 37 ℃ until a dense cell monolayer is obtained. Diluting the compound essential oil with culture medium to different concentrations of 600 μ L/L, 300 μ L/L, 150 μ L/L, 75 μ L/L, 37.5 μ L/L, and 18.75 μ L/L respectively; add to the cell culture plate 100. Mu.L/well. Each dilution was performed in duplicate in 3 wells with normal cell controls. Culturing in a 5% CO2 incubator at 37 deg.C, and detecting cell activity by MTS method, with specific results shown in attached Table 1. Each group of compound essential oil has no toxicity to VeroE6 cells at 300 mu L/L, so that 300 mu L/L is selected as the highest experimental concentration in subsequent experiments.

2. CPE observation: after 72h of virus infection, the normal cell control group has no CPE, the virus control group has obvious CPE, the groups with different essential oil concentrations show different inhibition effects on the CPE, the half effective concentration (IC) is calculated by observing cytopathic effect (CPE) and recording the experimental result by adopting a Reed-Muench method or GraphPad prism5.0₅₀) The results are shown in Table 2. All the compound essential oils have antiviral effects, compared with comparative example 1, example 1>5>2>3>4>6>9 are all significantly better than comparative example 1.

Claims

1. An antimicrobial essential oil composition, characterized in that it comprises, based on the total weight of the composition:

(1) 0.001-80% of one or more selected from carvacrol and thymol;

(2) 0.001-99.9% of one or more selected from lemon oil, sweet orange oil, bitter orange peel oil, white lemon oil and shaddock peel oil;

(3) 0.001-99.9% of one or more selected from the group consisting of peppermint oil, spearmint oil, peppermint oil and peppermint oil;

(4) 0.001-10% of one or more selected from laurel leaf oil and sweet origanum oil;

(5) 0.001-5% of one or more selected from patchouli oil, sandalwood oil and rosemary oil;

(6) 0.001-20% of one or more selected from geranium oil, pine needle oil and cedar wood oil; and

(7) Optionally, 0.001-20% of one or more selected from perilla oil, chrysanthemum morifolium oil and vitex leaf oil.

2. An antimicrobial essential oil composition, characterized in that it comprises, based on the total weight of the composition:

(1) 2-80% of one or more selected from carvacrol and thymol;

(2) 2-50% of one or more selected from lemon oil, sweet orange oil, bitter orange peel oil, lime oil and shaddock peel oil;

(3) 5-40% of one or more selected from peppermint oil, peppermint oil and peppermint oil;

(4) 2-20% of one or more selected from laurel leaf oil and sweet marjoram oil;

(5) 0.1-5% of one or more selected from patchouli oil, sandalwood oil and rosemary oil;

(6) 2-20% of one or more selected from geranium oil, pine needle oil and cedar wood oil; and

(7) Optionally, 2-15% of one or more selected from perilla oil, chrysanthemum morifolium oil and vitex leaf oil.

3. The antimicrobial essential oil composition according to claim 1, characterized in that the antimicrobial essential oil composition further optionally comprises one or more selected from the group consisting of:

(8) 0.0001-20% of honeysuckle flower oil;

(9) 0.0001-20% of rhizoma Ligustici Chuanxiong oil;

(10) 0.0001-20% of fructus Xanthii oil; and

(11) 0.0001-20% of weeping forsythia oil.

4. Antimicrobial essential oil composition according to claim 1, characterized in that it comprises:

(1) 5% carvacrol and 5% thymol;

(2) 35% lemon oil;

(3) 30% asian dementholized oil;

(4) 10% bay leaf oil;

(5) 0.5% of patchouli oil;

(6) 5% geranium oil; and

(7) 9.5% of perilla oil.

5. Antimicrobial essential oil composition according to claim 1, characterized in that it comprises:

(1) 0.005% carvacrol and 0.005% thymol;

(2) 0.1% lemon oil;

(3) 99.58% asian peppermint oil;

(4) 0.005% bay leaf oil;

(5) 0.25% patchouli oil;

(6) 0.005% geranium oil; and

(7) Perilla oil 0.05%.

6. Antimicrobial essential oil composition according to claim 1, characterized in that it comprises:

(1) 0.005% carvacrol and 0.005% thymol;

(2) 99.5% lemon oil;

(3) 0.25% peppermint oil asia;

(4) 0.005% bay leaf oil;

(5) 0.18% patchouli oil;

(6) 0.005% geranium oil; and

(7) Perilla oil 0.05%.

7. Antimicrobial essential oil composition according to claim 1, characterized in that it comprises:

(1) 51.25% carvacrol;

(2) 26.25% thymol;

(3) 8.75% lemon oil;

(4) 7.5% peppermint oil asia;

(5) 2.5% bay leaf oil;

(6) 0.125% patchouli oil;

(7) 1.25% geranium oil; and

(8) 2.375% perilla oil.

8. The antimicrobial essential oil composition of claim 3, characterized in that the antimicrobial essential oil composition comprises:

(1) 25% carvacrol and 25% thymol;

(2) 2% lemon oil;

(3) 10% peppermint oil asia;

(4) 20% bay leaf oil;

(5) 0.5% patchouli oil;

(6) 2% geranium oil;

(7) 5% of honeysuckle oil;

(9) 4% of ligusticum oil;

(10) 0.3% xanthium oil; and

(11) 0.2% of weeping forsythia oil.

9. Antimicrobial essential oil composition according to any one of claims 1 to 8, characterized in that the microorganism is selected from the group consisting of bacteria, fungi, viruses and combinations thereof.

10. Antimicrobial essential oil composition according to any one of claims 1 to 8, characterized in that the microorganism is a neocoronavirus.

11. A product for reducing or killing microorganisms on one or more of a hard surface, a textile, skin, a mucous membrane or hair, characterized in that the product comprises the antimicrobial essential oil composition according to any one of claims 1-8 and optionally one or more adjuvants.

12. The product of claim 11, characterized in that the product is one or more selected from the group consisting of a disinfectant product, a commodity, a protective product, and a food product.

13. The product of claim 12, wherein the consumer product is one or more selected from the group consisting of a cosmetic product, a household care product, and a personal care product, wherein the consumer product comprises one or more of a mask pop, an air freshener, a mouth spray, a hand sanitizer, an aerogel, a bacteriostatic spray, a sanitary wipe, a surface cleaner, and a detergent; the food is one or more of chewing gum, popping beads, gel candy, hard candy, soft candy, beverage and dietary supplement; the protective articles comprise one or more of a mask bead explosion mask, a civil mask, a medical mask, protective gloves and protective clothes.

14. A process for preparing the antimicrobial essential oil composition of any one of claims 1-8, comprising: all ingredients of the antimicrobial essential oil composition are mixed homogeneously.

15. The method of claim 14, characterized in that the mixing is carried out at ambient temperature and pressure.

16. A method of manufacturing a mask according to claim 13 comprising preparing the antimicrobial essential oil composition of any one of claims 1 to 8 into essential oil concentrate or essential oil microcapsule, and manufacturing the mask according to a conventional method.