CN115202025A - 具有倾斜照明的显微镜系统 - Google Patents

具有倾斜照明的显微镜系统 Download PDF

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Publication number
CN115202025A
CN115202025A CN202210382469.0A CN202210382469A CN115202025A CN 115202025 A CN115202025 A CN 115202025A CN 202210382469 A CN202210382469 A CN 202210382469A CN 115202025 A CN115202025 A CN 115202025A
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CN
China
Prior art keywords
plane
lens
objective lens
sample
light
Prior art date
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Pending
Application number
CN202210382469.0A
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English (en)
Chinese (zh)
Inventor
J·奥雷奥普洛斯
P·辛克莱
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Andor Technology Co ltd
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Andor Technology Co ltd
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Publication of CN115202025A publication Critical patent/CN115202025A/zh
Pending legal-status Critical Current

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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/082—Condensers for incident illumination only
    • G02B21/084—Condensers for incident illumination only having annular illumination around the objective
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/10—Condensers affording dark-field illumination
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/02—Objectives
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/082—Condensers for incident illumination only
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/12—Condensers affording bright-field illumination
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/12—Condensers affording bright-field illumination
    • G02B21/125—Condensers affording bright-field illumination affording both dark- and bright-field illumination
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/0001—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings specially adapted for lighting devices or systems
    • G02B6/0005—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings specially adapted for lighting devices or systems the light guides being of the fibre type
    • G02B6/0006—Coupling light into the fibre
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/56—Optics using evanescent waves, i.e. inhomogeneous waves

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Microscoopes, Condenser (AREA)
CN202210382469.0A 2021-04-13 2022-04-13 具有倾斜照明的显微镜系统 Pending CN115202025A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB2105232.9A GB2605793B (en) 2021-04-13 2021-04-13 Microscope system with oblique illumination
GB2105232.9 2021-04-13

Publications (1)

Publication Number Publication Date
CN115202025A true CN115202025A (zh) 2022-10-18

Family

ID=75949391

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210382469.0A Pending CN115202025A (zh) 2021-04-13 2022-04-13 具有倾斜照明的显微镜系统

Country Status (5)

Country Link
US (1) US20220326498A1 (https=)
EP (1) EP4075181A1 (https=)
JP (1) JP2022162999A (https=)
CN (1) CN115202025A (https=)
GB (1) GB2605793B (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116679432A (zh) * 2023-04-11 2023-09-01 南方科技大学 一种物镜后焦面成像系统及方法

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023057348A1 (en) * 2021-10-05 2023-04-13 Leica Microsystems Cms Gmbh Sample carrier and method for imaging a sample
CN114324436A (zh) * 2021-12-23 2022-04-12 江苏大学 一种微纳结构的激光加工与实时原位高分辨观测装置
US12422661B2 (en) * 2023-01-27 2025-09-23 POSTECH Research and Business Development Foundation Photographing apparatus and photographing method
CN116400558A (zh) * 2023-03-20 2023-07-07 中国科学院上海光学精密机械研究所 基于波长-时间映射的超快成像装置
DE102024127806A1 (de) 2024-09-25 2026-03-26 Carl Zeiss Microscopy Gmbh Mikroskop und verfahren zur mikroskopie
CN119438158B (zh) * 2024-11-11 2025-11-18 湖北大学 一种基于物镜光轴偏离显微镜主光轴实现超分辨光学显微成像的方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110134519A1 (en) * 2009-12-08 2011-06-09 Spectral Applied Research Inc. Imaging Distal End of Multimode Fiber
US20150049376A1 (en) * 2012-04-20 2015-02-19 Hamamatsu Photonics K.K. Beam-shaping device
US20150131142A1 (en) * 2012-04-20 2015-05-14 Hamamatsu Photonics K.K. Beam expander
EP2977810A1 (de) * 2014-07-22 2016-01-27 Leica Microsystems CMS GmbH Verfahren und vorrichtung zum mikroskopischen untersuchen einer probe
WO2020224882A1 (en) * 2019-05-06 2020-11-12 Asml Netherlands B.V. Dark field microscope

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7433563B2 (en) * 2005-05-25 2008-10-07 University Of Vermont And State Agricultural College Optical fiber microscopy launch system and method
DE102008034137A1 (de) * 2007-09-28 2009-04-02 Carl Zeiss Microlmaging Gmbh Mikroskop und Verfahren zum Betreiben eines Mikroskops
DE102009037366A1 (de) * 2009-08-13 2011-02-17 Carl Zeiss Microlmaging Gmbh Mikroskop, insbesondere zur Messung von Totalreflexions-Fluoreszenz, und Betriebsverfahren für ein solches
US9500847B2 (en) * 2012-10-12 2016-11-22 Spectral Applied Research Inc. Total internal reflectance fluorescence (TIRF) microscopy across multiple wavelengths simultaneously
JP6540823B2 (ja) * 2015-11-27 2019-07-10 株式会社ニコン 顕微鏡、観察方法、及び制御プログラム
JP7112686B2 (ja) * 2018-07-18 2022-08-04 株式会社エビデント 標本観察装置
DE102018009056A1 (de) * 2018-11-12 2020-05-14 Carl Zeiss Microscopy Gmbh Beschleunigte Verfahren und Vorrichtungen für die dreidimensionale Mikroskopie mit strukturierter Beleuchtung
GB2585072B (en) * 2019-06-27 2024-01-24 Andor Tech Limited Radiation Delivery apparatus for microscope systems

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110134519A1 (en) * 2009-12-08 2011-06-09 Spectral Applied Research Inc. Imaging Distal End of Multimode Fiber
US20150049376A1 (en) * 2012-04-20 2015-02-19 Hamamatsu Photonics K.K. Beam-shaping device
US20150131142A1 (en) * 2012-04-20 2015-05-14 Hamamatsu Photonics K.K. Beam expander
EP2977810A1 (de) * 2014-07-22 2016-01-27 Leica Microsystems CMS GmbH Verfahren und vorrichtung zum mikroskopischen untersuchen einer probe
WO2020224882A1 (en) * 2019-05-06 2020-11-12 Asml Netherlands B.V. Dark field microscope

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116679432A (zh) * 2023-04-11 2023-09-01 南方科技大学 一种物镜后焦面成像系统及方法

Also Published As

Publication number Publication date
JP2022162999A (ja) 2022-10-25
GB2605793B (en) 2025-04-30
US20220326498A1 (en) 2022-10-13
GB202105232D0 (en) 2021-05-26
GB2605793A (en) 2022-10-19
EP4075181A1 (en) 2022-10-19

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