CN115177565A

CN115177565A - Whitening and skin-brightening composition and application thereof in cosmetics

Info

Publication number: CN115177565A
Application number: CN202210895898.8A
Authority: CN
Inventors: 许锐林; 李楚忠; 陈庆生
Original assignee: Guangzhou Huanya Cosmetic Science and Technology Co Ltd
Current assignee: Guangzhou Huanya Cosmetic Science and Technology Co Ltd
Priority date: 2022-07-27
Filing date: 2022-07-27
Publication date: 2022-10-14
Anticipated expiration: 2042-07-27
Also published as: CN115177565B

Abstract

The invention discloses a whitening and skin-brightening composition and application thereof in cosmetics; according to the invention, through researching the skin blackening principle and the whitening action mechanism, three components of the black willow bark extract, the palm leaf tree extract and the vegetarian square flower extract with corresponding effects are screened from a plurality of plant components, and through a plurality of research experiments, scientific and reasonable compounding proportion, synergistic effect and complementation are obtained, and the whitening route has the effects of resisting sunlight oxidative damage, inhibiting tyrosinase activity and transfer and reducing pigmentation, and plays an inhibiting role in various aspects of skin blackening, so that the aims of comprehensive, efficient and safe whitening are fulfilled. The composition is mild and non-irritant, the tyrosinase inhibition rate and the hydroxyl radical removal rate are respectively more than 85% and 82%, and the prepared whitening cream has an obvious pigment reduction effect and has good whitening and skin brightening effects; can be further made into cosmetic lotion, emulsion, cream, facial mask, spray, essence, facial cleanser, sunscreen cream, etc.

Description

Whitening and skin-brightening composition and application thereof in cosmetics

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a whitening and skin-brightening composition and application thereof in cosmetics.

Background

With the aging, the skin is inevitably aged, and problems such as dryness, looseness, darkness and the like occur, and external factors such as sunlight irradiation and environmental pollution also increase the problems. With the increase of the consumption level of people and the enhancement of the safety and health consciousness, functional cosmetics such as whitening, anti-aging, repairing and the like are increasingly sought after. In the increasingly competitive market of functional cosmetics, it is desired to take a place and think about developing more powerful, comprehensive and mild cosmetics. Most of the traditional whitening products achieve whitening through a mode of inhibiting tyrosinase activity, although the traditional whitening products have certain effects, the traditional whitening products have single path, are easy to repeat and have long action time, and the conventional whitening agents have the risk of stimulating the skin if the dosage of the conventional whitening agents is large. To achieve the actual whitening, it is necessary to consider the aspects of preventing oxidative damage due to sunlight irradiation, removing already-produced pigments, and the like, and to prevent and repair only in each way of causing skin blackening, so that the original whitening can be achieved only by inhibiting the activity of tyrosinase. Therefore, it is a matter of great interest to develop mild products with better and more comprehensive whitening effects.

Disclosure of Invention

The invention aims to provide a mild and efficient whitening and skin-brightening composition, which is different from the traditional whitening, and realizes comprehensive, efficient and mild whitening effects through multiple ways of resisting photodamage, inhibiting tyrosinase activity and transfer and reducing pigmentation by selecting and compounding natural plant extracts.

The technical scheme adopted by the invention is as follows:

in a first aspect of the invention, there is provided a composition comprising the following ingredients: black willow bark extract, palm leaf tree extract and vegetarian square flower extract.

The black willow bark extract is from black willow, contains abundant tannic acid and salicylic acid, belongs to natural salicylic acid, has the same effect as other synthetic acids, but does not generate any side effect, such as: and (4) irritation. The skin care product has the effects of enhancing cell activity and improving cell regeneration, and can be used for peeling off and renewing skin cutin and reducing skin pigmentation, so that the effects of brightening and whitening are realized.

The palm leaf tree extract is rich in xylose and galactooligosaccharide in palmate red skin algae, and melanin generation is limited by inhibiting the activity of tyrosinase and the quantity of synthesized melanin; reducing synthesis of protein-anchored complexes essential for melanosome transport to keratinocytes; inhibit the synthesis of stem cell factor, control the pigmentation induced by light, and make the skin brighter and more delicate.

The extract of the jasminum grandiflorum is rich in jasmine flavonoids, protects the activity of catalase, helps to recover the content of glutathione in cells, can strengthen the function of an endogenous anti-free radical system of skin, resists light damage, effectively protects protein and DNA, reduces inflammation, reduces the amount of synthesized melanin, and realizes the effects of radically whitening and brightening the skin.

In some embodiments of the invention, the composition comprises, in mass percent: 1-20% of salix nigra bark extract, 0.1-10% of palm leaf tree extract and 0.1-10% of vegetarian square flower extract.

In some preferred embodiments of the present invention, the composition comprises, in mass percent: 1-10% of salix nigra bark extract, 1-10% of palm leaf tree extract and 1-10% of vegetarian square flower extract.

In some more preferred embodiments of the present invention, the composition comprises, in mass percent: 5-10% of salix nigra bark extract, 1-5% of palm leaf tree extract and 1-5% of vegetarian square flower extract.

In some embodiments of the present invention, the whitening and skin-lightening composition is prepared by: mixing the Salix nigra bark extract, the palm leaf tree extract and the vegetarian square flower extract uniformly to obtain the composition.

In a second aspect of the invention, there is provided the use of a composition according to the first aspect of the invention in the manufacture of a product.

In some embodiments of the invention, the product functions as at least one of (1) to (6):

(1) Whitening;

(2) Lightening the skin color;

(3) Inhibiting tyrosinase activity and transfer;

(4) Reducing pigmentation;

(5) Sunlight oxidation damage is resisted;

(6) Improve the hydroxyl radical clearance rate.

In some embodiments of the invention, the product is a cosmetic product.

In some embodiments of the present invention, the cosmetic includes, but is not limited to, lotions, milks, creams, masks, sprays, serums, facial cleansers.

In a third aspect of the invention, there is provided a product comprising a composition according to the first aspect of the invention.

In some embodiments of the invention, the composition is present in the product in an amount of 0.1 to 10% by weight.

In some preferred embodiments of the invention, the composition is present in the product in an amount of 0.5 to 5% by weight.

In some embodiments of the invention, the product further comprises an adjuvant or carrier.

In some embodiments of the invention, the adjuvant comprises at least one of a humectant, an emulsifier, an emollient, a preservative, an antioxidant, a fragrance, a thickener, a chelating agent, a solubilizer, a pH adjuster.

In some embodiments of the invention, the moisturizer comprises: at least one of glycerol, butanediol, propylene glycol, 1,3-propanediol, 1,2-pentanediol, sodium hyaluronate, 1,2-hexanediol.

In some embodiments of the invention, the emulsifier comprises: PEG-100 stearate, glyceryl stearate, PEG-40 stearate, polysorbate-60, methylglucose hemistearate, polysorbate-20, C20-22 alcohol phosphate, C12-20 alkyl glucoside, arachidonol glucoside, polyglyceryl-3 methyl glucose distearate, lecithin, hydrogenated lecithin, ceteareth-12, steareth-2, steareth-21, PEG-20 methyl glucose hemistearate, sorbitan sesquioleate, lauryl PEG/PPG-18/18 methicone, lauryl PEG-9 dimethiconoethyl dimethicone, PEG-10 dimethicone, dimethicone PEG-10/15 crosspolymer, cetearyl olivate, sorbitan olivate, PEG-30 dipolyhydroxystearate, cetyl PEG/PPG-10/1 dimethicone, polyglyceryl-2 dipolyhydroxystearate, polyglyceryl-3 diisostearate, cetearyl glucoside, sodium stearyl glutamate, potassium cetyl phosphate.

In some embodiments of the invention, the emollient comprises: jojoba seed oil, polydimethylsiloxane, hydrogenated polyisobutylene, cyclopentadimethylsiloxane, shea butter, C12-15 alcohol benzoate, isohexadecane, octyl methicone, phenyl trimethicone, dioctyl carbonate, pentaerythritol tetrakis (ethyl hexanoate), coconut oil, squalane, olive oil, meadowfoam seed oil, propyl heptyl caprylate, tri (ethyl hexanoate), caprylic/capric triglyceride, isononyl isononanoate.

In some embodiments of the invention, the preservative comprises: at least one of methyl hydroxybenzoate, propyl hydroxybenzoate, phenoxyethanol, sodium benzoate, and potassium sorbate.

In some embodiments of the invention, the antioxidant comprises: at least one of p-hydroxyacetophenone and tocopherol acetate.

In some embodiments of the invention, the thickener comprises: at least one of disteardimonium hectorite, beeswax, ozokerite, stearyl alcohol, cetyl alcohol, cetearyl alcohol, behenyl alcohol, stearic acid, behenic acid, hectorite, xanthan gum, gum arabic, hydroxyethyl cellulose, acrylic acid/C10-30 alkanol acrylate crosspolymer, hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, polyacrylate crosspolymer-6, sodium polyacrylate, carbomer, ammonium acryloyldimethyl taurate/VP copolymer.

In some embodiments of the invention, the chelating agent comprises: disodium EDTA.

In some embodiments of the present invention, the solubilizing agent comprises: at least one of PEG-60 hydrogenated castor oil, PEG-40 hydrogenated castor oil, PPG-26-Butaneth-26.

In some embodiments of the invention, the pH adjusting agent comprises: at least one of aminomethyl propanol, arginine, citric acid, sodium citrate, and triethanolamine.

The invention has the beneficial effects that:

according to the invention, through researching the skin blackening principle and the whitening action mechanism, three components of reasonable black willow bark extract, palm leaf tree extract and vegetarian square flower extract with corresponding effects are screened from a plurality of plant components, and through a plurality of research tests, scientific and reasonable compounding proportion, synergistic effect and complementation are obtained, so that the whitening route of three key whitening routes of resisting sunlight oxidative damage, inhibiting tyrosinase activity and transfer and reducing pigmentation is achieved, the inhibiting effect is exerted on all aspects of skin blackening, and the whitening purpose of comprehensive, high efficiency and safety is realized. The composition is mild and non-irritant, the tyrosinase inhibition rate and the hydroxyl radical clearance rate are respectively more than 85% and 82%, and the prepared whitening cream has an obvious pigment reduction effect and has good whitening and skin brightening effects; can be further made into cosmetic lotion, emulsion, cream, facial mask, spray, essence, facial cleanser, sunscreen cream, etc.

Detailed Description

The concept and technical effects of the present invention will be clearly and completely described below in conjunction with the embodiments to fully understand the objects, features and effects of the present invention. It is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and those skilled in the art can obtain other embodiments without inventive effort based on the embodiments of the present invention, and all embodiments are within the protection scope of the present invention.

The raw materials, reagents or apparatus used in the following examples, in which the bark extract of black willow is derived from Active concentrates, LLC, are either conventionally commercially available or may be obtained by methods known in the art, unless otherwise specified; the palm leaf tree extract is from Guangzhou Norgnonia Biotechnology GmbH; the extract of maigre's flowers is from SILAB s.a., but is not intended to limit the present invention, and examples of the extract of maigre's flowers include black willow bark, palm leaf tree, and maigre's flowers, which are obtained by purchasing the extract from regular suppliers of raw materials, or from raw materials purchased from conventional commercial sources, and then subjecting the extract to aqueous or alcoholic extraction.

Example 1

The composition comprises the following components in percentage by mass: 8% of salix nigra bark extract, 3% of palm leaf tree extract, 3% of vegetarian square flower extract and the balance of deionized water.

Example 2

The composition comprises the following components in percentage by mass: 5% of salix nigra bark extract, 5% of palm leaf tree extract, 5% of vegetarian square flower extract and the balance of deionized water.

Example 3

The composition comprises the following components in percentage by mass: 10% of salix nigra bark extract, 1% of palm leaf tree extract, 1% of vegetarian square flower extract and the balance of deionized water.

Example 4

The composition comprises the following components in percentage by mass: 5% of salix nigra bark extract, 5% of palm leaf tree extract, 1% of vegetarian square flower extract and the balance of deionized water.

Example 5

The composition comprises the following components in percentage by mass: 5% of salix nigra bark extract, 1% of palm leaf tree extract, 5% of vegetarian square flower extract and the balance of deionized water.

Comparative example 1

And setting a combination form of which the composition ratio is not in the protection range to verify the reasonability of the composition ratio. The composition comprises the following components in percentage by mass: 0.5% of salix nigra bark extract, 12% of palm leaf tree extract, 12% of vegetarian square flower extract and the balance of deionized water.

Comparative example 2

And setting a combination form of which the composition ratio is not in the protection range to verify the reasonability of the composition ratio. The composition comprises the following components in percentage by mass: 25% of black willow bark extract, 0.05% of palm leaf tree extract, 0.05% of vegetarian square flower extract and the balance of deionized water.

Comparative example 3

The combination form of removing a certain plant component of the composition is set to verify the reasonability of the composition combination. The composition comprises the following components in percentage by mass: palm leaf tree extract 3%, vegetarian square flower extract 3%, and the balance of deionized water.

Comparative example 4

The combination form of removing a certain plant component of the composition is set to verify the reasonability of the composition combination. The composition comprises the following components in percentage by mass: 8% of black willow bark extract, 3% of vegetarian square flower extract and the balance of deionized water.

Comparative example 5

The combination form of removing a certain plant component of the composition is set to verify the reasonability of the composition combination. The composition comprises the following components in percentage by mass: 8% of black willow bark extract, 3% of palm leaf tree extract and the balance of deionized water.

Comparative example 6

The combination form of a certain plant component of the composition is reserved to verify the reasonability of the combination of the composition. The composition comprises the following components in percentage by mass: 8% of black willow bark extract and the balance of deionized water.

Comparative example 7

The combination form of a certain plant component of the composition is reserved to verify the reasonability of the combination of the composition. The composition comprises the following components in percentage by mass: palm leaf tree extract 3%, and the balance of deionized water.

Comparative example 8

The combination form of reserving a certain plant component of the composition is set to verify the reasonability of the composition combination. The composition comprises the following components in percentage by mass: 3% of the extract of the vegetarian square flower and the balance of deionized water.

Comparative example 9

The rationality of the composition is verified by replacing any one of the plant components in the composition with a common whitening plant component (licorice root extract). The composition comprises the following components in percentage by mass: 8% of licorice root extract, 3% of palm leaf tree extract, 3% of vegetarian square flower extract and the balance of deionized water.

Comparative example 10

The rationality of the composition is verified by replacing any one of the plant components in the composition with a common whitening plant component (licorice root extract). The composition comprises the following components in percentage by mass: 8% of black willow bark extract, 3% of licorice root extract, 3% of vegetarian square flower extract and the balance of deionized water.

Comparative example 11

The rationality of the composition is verified by replacing any one of the plant components in the composition with a common whitening plant component (licorice root extract). The composition comprises the following components in percentage by mass: 8% of black willow bark extract, 3% of palm leaf tree extract, 3% of licorice root extract and the balance of deionized water.

Example 6

The composition of example 1 was used to prepare a whitening lotion, and the formulation was as shown in table 1.

TABLE 1

The preparation method comprises the following steps:

(1) Sequentially adding the phase A raw materials into a stirring pot, heating to 60 ℃, homogenizing, stirring and dissolving uniformly;

(2) Heating the phase B raw material to 60 ℃, dissolving and transparent, adding the phase B raw material into a stirring pot, uniformly dispersing, and cooling to 40 ℃;

(3) Adding the C-phase raw material into a stirring pot, stirring and dispersing uniformly;

(4) And (3) uniformly dispersing the phase D raw material, adding the dispersed phase D raw material into a stirring pot, uniformly dispersing, and cooling to below 37 ℃ to obtain the whitening toning lotion.

Example 7

The composition of example 1 was used to prepare a whitening mask, the formulation of which is shown in table 2.

TABLE 2

The preparation method comprises the following steps:

(2) Adding the phase B raw material into a stirring pot, and homogenizing and dispersing uniformly;

(3) Heating the C-phase raw material to 60 ℃, dissolving and transparent, adding the C-phase raw material into a stirring pot, uniformly dispersing, and cooling to 40 ℃;

(3) Adding the D-phase raw material into a stirring pot, stirring and dispersing uniformly;

(4) And (3) uniformly dispersing the phase E raw material, adding the mixture into a stirring pot, uniformly dispersing, and cooling to below 37 ℃ to obtain the whitening mask.

Example 8

The composition of example 1 was used to prepare a whitening emulsion having the formulation shown in table 3.

TABLE 3

The preparation method comprises the following steps:

(1) Sequentially adding the phase A raw materials into an emulsifying pot, heating to 80 ℃, homogenizing, stirring and dissolving uniformly;

(2) Sequentially adding the phase B raw materials into an oil phase pot, heating to 80 ℃, and uniformly stirring and dispersing;

(3) Adding the phase B raw material into an emulsifying pot, homogenizing and emulsifying, uniformly stirring, and cooling to 60 ℃;

(4) Adding the C-phase raw material into an emulsifying pot, and homogenizing and dispersing uniformly;

(5) Heating the D-phase raw material to 60 ℃, dissolving and transparent, adding the D-phase raw material into an emulsifying pot, dispersing uniformly, and cooling to 40 ℃;

(6) And (3) uniformly dispersing the phase E raw material, adding the E raw material into a stirring pot, uniformly dispersing, and cooling to below 37 ℃ to obtain the whitening emulsion.

Example 9

The composition of example 1 was used to prepare a whitening cream, the formulation is shown in table 4.

TABLE 4

The preparation method comprises the following steps:

(4) Adding the C-phase raw material into an emulsifying pot, and homogenizing and uniformly dispersing;

(6) And (3) uniformly dispersing the phase E raw material, adding the E raw material into a stirring pot, uniformly dispersing, and cooling to below 37 ℃ to obtain the whitening cream.

Comparative example 12

A blank control cream without the whitening composition of example 1 was prepared, and the proportions of the remaining ingredients and the preparation method were kept the same as in example 9.

Test example 1

Erythrocyte hemolysis assay:

the ocular stimulatory effect of the samples was assessed by an erythrocyte hemolysis experiment. Taking 500 mu L of 2% erythrocyte suspension and 500 mu L of sample solution with different concentrations (diluted by PBS) for equal volume mixing, simultaneously taking equal volume mixing of physiological saline and 2% erythrocyte suspension as a negative group, taking equal volume mixing of 1% SDS solution and 2% erythrocyte suspension as a positive group, arranging three groups in parallel for each group, placing in a 37 ℃ biochemical incubator for standing culture for 3h, taking 10 mu L of supernatant and 90 mu L of physiological saline to carry out absorbance value determination at 410, 540 and 575nm in a 96-well plate.

Hemolysis rate = (a) _{Sample set 410nm} -A _{Negative control group 410nm} )/(A _{Positive control group 410nm} -A _{Negative control group 410nm} )×100％

Protein Denaturation Index (DI) = (a) _{Sample set 575nm} -A _{Sample set 540nm} )/(A _{Positive control group 575nm} -A _{Positive control group 540nm} )×100％

Determining a regression equation from the hemolysis rate plot, and calculating the sample mass concentration HC at which the test sample causes 50% hemolysis of the red blood cells ₅₀ (mg/L) and DI value at 1% volume fraction of sample. Calculate the L/D value of the sample from the HC50 value and the DI value: L/D = HC ₅₀ and/DI. The degree of irritation of the test samples was graded according to the grading standards for erythrocyte hemolysis test of the European Alternatives validation center (ECVAM). As shown in table 5.

TABLE 5

L/D	Grading
		﹥100	Has no irritation
10﹤L/D≤100	Micro-stimulation property
		1﹤L/D≤10	Mild irritation
0.1﹤L/D≤1	Moderate irritation
		L/D≤0.1	Severe irritation

The results of the erythrolysis experiments of the whitening and skin-lightening compositions prepared in examples 1 to 5 and the compositions of comparative examples 1 to 11 are shown in table 6.

TABLE 6

Sample name	HC ₅₀ (mg/L)	DI(％)	L/D	Evaluation of
					Example 1	33901.4	78.45	432.14	Has no irritation
Example 2	28799.7	76.83	374.85	Has no irritation
					Example 3	18579.0	80.61	230.48	Has no irritation
Example 4	22662.4	73.18	309.68	Has no irritation
					Example 5	15626.9	83.45	187.26	Has no irritation
Comparative example 1	21091.7	89.47	235.74	Has no irritation
					Comparative example 2	6168.3	94.36	65.37	Micro-stimulation property
Comparative example 3	8713.0	88.26	98.72	Micro-stimulation property
					Comparative example 4	7230.0	85.74	84.32	Micro-stimulation property
Comparative example 5	8449.0	80.96	104.36	Has no irritation
					Comparative example 6	12333.9	78.64	156.84	Has no irritation
Comparative example 7	5751.0	79.16	72.65	Micro-stimulation property
					Comparative example 8	7939.2	87.10	91.15	Micro-stimulation property
Comparative example 9	1290.9	82.38	15.67	Micro-stimulation property
					Comparative example 10	5513.2	92.69	59.48	Micro-stimulation property
Comparative example 11	3444.4	93.65	36.78	Micro-stimulation property

As can be seen from the results of the erythrocyte hemolysis test in table 6, the skin whitening and brightening compositions prepared in examples 1 to 5 of the present invention are non-irritating, while the compositions in comparative examples except 1, 5 and 6 are non-irritating and micro-irritating. The whitening and skin-brightening composition has the characteristics of being mild and non-irritant compared with other composition forms.

Test example 2

Tyrosinase activity inhibition test:

adding samples according to the table 7, taking L-tyrosine as a reaction substrate, setting four groups of sample solutions shown in the table 7, respectively adding PBS buffer solution, 0.5 g/L-tyrosine solution and samples with different proportions, setting 3 parallel samples for each sample, placing the samples in a water bath at 37 ℃ for constant temperature for 10min, adding 100U/mL tyrosinase solution, mixing uniformly, placing the samples in the water bath at 37 ℃ for reaction for 10min, quickly transferring the samples into a cuvette, and measuring the absorbance value A at 475nm ₁ 、A ₂ 、A ₃ 、A ₄ . The inhibition rate of the tyrosinase activity by the sample was calculated according to the following formula.

In vitro tyrosinase activity inhibition rate = (1- (A) ₄ -A ₃ )/(A ₂ -A ₁ ))*100％。

TABLE 7

	Blank control/mL	blank/mL	Sample control/mL	sample/mL
					Sample (I)	--	--	0.8	0.8
PBS(pH＝6.8)	1.8	1.4	1.0	0.6
					L-tyrosine	0.8	0.8	0.8	0.8
Tyrosinase enzyme	--	0.4	--	0.4
					Total amount of	2.6	2.6	2.6	2.6

The whitening and skin-lightening compositions of examples 1 to 5 and the compositions of comparative examples 1 to 11 were used to determine the inhibition rate of tyrosinase activity, and the results are shown in table 8.

TABLE 8

Sample (I)	Tyrosinase inhibition/%)
		Example 1	94.45
Example 2	90.51
		Example 3	92.15
Example 4	88.26
		Example 5	85.69
Comparative example 1	72.56
		Comparative example 2	79.32
Comparative example 3	67.52
		Comparative example 4	54.96
Comparative example 5	63.04
		Comparative example 6	43.42
Comparative example 7	53.15
		Comparative example 8	62.85
Comparative example 9	70.59
		Comparative example 10	76.37
Comparative example 11	80.46

As can be seen from the data in Table 8, the tyrosinase inhibition rates of the skin whitening and brightening compositions of examples 1-5 are all above 85%, which indicates that the compositions have good tyrosinase inhibition rates, and the tyrosinase inhibition rates of the compositions in the comparative examples are kept at a lower level no matter the compositions are out of the content range (comparative examples 1 and 2) or do not contain the ingredients (comparative examples 3-8) completely disclosed by the invention or the compositions replace any plant ingredient of the compositions by a conventional whitening ingredient (licorice root extract), which indicates that the compositions disclosed by the invention are scientific and reasonable in matching and proportioning.

Test example 3

Hydroxyl radical scavenging effect test:

the amounts of the solutions added were as shown in Table 9, and 9mmoL/L of FeSO was added to the cuvette in the order mentioned ₄ 9mmoL/L ethanol-salicylic acid, then adding a proper amount of deionized water, and finally adding 8.8mmoL/L H ₂ O ₂ Then shaking up, heating in water bath at 37 ℃ for 15 minutes, taking out, and measuring the absorbance A of the mixture ₀ 。A ₀ During measurement, the reference solution is a system without adding hydrogen peroxide. The absorbance A was measured as described above by adding each solution as shown in Table 9, each set being arranged in three parallel groups _X 、A _X0 。A _X 、A _X0 During measurement, the reference solution is deionized water.

TABLE 9

Calculating the formula: hydroxyl radical clearance (%) = a ₀ -(A _X -A _X0 )/A ₀ *100% of them being A ₀ Absorbance of blank control; a. The _X Adding the light absorption value of the sample; a. The _X0 Without adding a color-developing agent H ₂ O ₂ Background absorbance of the solution.

The whitening and skin-lightening compositions of examples 1 to 5 and the compositions of comparative examples 1 to 11 were used to measure the removal rate of hydroxy radicals, and the results are shown in table 10.

Watch 10

Sample (I)	Hydroxy radical clearance/%)
		Example 1	92.16
Example 2	90.26
		Example 3	91.63
Example 4	88.46
		Example 5	82.06
Comparative example 1	65.87
		Comparative example 2	75.24
Comparative example 3	68.34
		Comparative example 4	59.21
Comparative example 5	73.64
		Comparative example 6	70.31
Comparative example 7	69.31
		Comparative example 8	76.25
Comparative example 9	80.36
		Comparative example 10	82.41
Comparative example 11	76.29

As can be seen from the data in table 10, the hydroxyl radical removal rates of the whitening and skin-brightening compositions of examples 1 to 5 are all above 82%, which indicates that the whitening and skin-brightening compositions have very good hydroxyl radical removal rates, while the hydroxyl radical removal rates of the comparative examples are not substantially higher than those of the examples except for comparative example 10, which indicates that the whitening and skin-brightening compositions used in the examples have synergistic and synergistic effects, and therefore, the whitening and skin-brightening compositions of the invention have better effects under the compositions and ratios.

Test example 4

Human body whitening test:

in order to eliminate other interferences and verify the whitening effect of the composition, the applicant selects 10 volunteers with healthy skin and no skin care product allergy history, and takes the left and right half-face areas as the tested parts, and applies whitening cream of example 9 on one side and blank cream of comparative example 12 on the other side. It is applied 1 times in the morning and at night for 4 weeks, and is prevented from washing off. The melanin content in the skin was examined with a skin melanin tester before use and after every 1 week, respectively. The results are shown in Table 11.

TABLE 11

As can be seen from the results in table 11, the whitening cream of example 9 has better depigmenting ability, and the melanin content of the facial skin is generally reduced by about 20 after the cream is used by consumers for four weeks, while the melanin content of the blank cream of comparative example 12 fluctuates from the initial value under the same conditions, and the change is not obvious. It is demonstrated that the whitening and skin-lightening composition prepared in example 1 has a significant effect of reducing pigmentation.

In conclusion, the whitening and skin-brightening composition provided by the invention has the synergistic complementation and synergy effects through scientific and reasonable matching. The composition has the characteristics of mildness and no stimulation, and the tyrosinase inhibition effect and the hydroxyl radical removal effect of the composition are obviously superior to those of a single or incomplete component or a comparative composition which is not in a protection content range or replaces components. The whitening cream is added into a cream matrix to test the whitening effect of a human body, and the result shows that the whitening cream has good capability of reducing pigmentation compared with a blank cream.

The present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art. Furthermore, the embodiments of the present invention and features of the embodiments may be combined with each other without conflict.

Claims

1. A composition, comprising the following ingredients: black willow bark extract, palm leaf tree extract and vegetarian square flower extract.

2. The composition according to claim 1, wherein the whitening and skin-lightening composition comprises, in mass percent: 1-20% of salix nigra bark extract, 0.1-10% of palm leaf tree extract and 0.1-10% of vegetarian square flower extract.

3. The composition according to claim 1, wherein the whitening and skin-lightening composition comprises, in mass percent: 1-10% of salix nigra bark extract, 1-10% of palm leaf tree extract and 1-10% of vegetarian square flower extract.

4. Use of a composition according to any one of claims 1 to 3 for the preparation of a product; preferably, the function of the product is at least one of (1) to (6):

(1) Whitening;

(2) Lightening the skin color;

(3) Inhibiting tyrosinase activity and transfer;

(4) Reducing pigmentation;

(5) Sunlight oxidation damage is resisted;

(6) Improve the hydroxyl radical clearance rate.

5. The use according to claim 4, wherein the product is a cosmetic product, preferably the cosmetic product comprises but is not limited to lotions, milks, creams, masks, sprays, essences, facial washes, sunscreens.

6. A product comprising a composition according to any one of claims 1 to 3.

7. The product according to claim 6, characterized in that the composition is present in the product in a mass percentage of 0.1 to 10%.

8. The product of claim 7, further comprising an adjuvant or a carrier.

9. The product of claim 8, wherein the adjuvant comprises at least one of a moisturizer, an emulsifier, an emollient, a preservative, an antioxidant, a fragrance, a thickener, a chelating agent, a solubilizer, and a pH adjuster.

10. The product of claim 9,

the humectant includes: at least one of glycerol, butanediol, propylene glycol, 1,3-propanediol, 1,2-pentanediol, sodium hyaluronate, 1,2-hexanediol;

the emulsifier comprises: PEG-100 stearate, glyceryl stearate, PEG-40 stearate, polysorbate-60, methylglucose hemistearate, polysorbate-20, C20-22 alcohol phosphate, C12-20 alkyl glucoside, arachidonol glucoside, polyglyceryl-3 methyl glucose distearate, lecithin, hydrogenated lecithin, ceteareth-12, steareth-2, steareth-21, PEG-20 methyl glucose hemistearate, sorbitan sesquioleate, lauryl PEG/PPG-18/18 methicone, lauryl PEG-9 dimethiconoethyl dimethicone, PEG-10 dimethicone, dimethicone PEG-10/15 crosspolymer, cetearyl olivate, sorbitan olivate, PEG-30 dipolyhydroxystearate, cetyl PEG/PPG-10/1 dimethicone, polyglyceryl-2 dipolyhydroxystearate, polyglyceryl-3 diisostearate, cetearyl glucoside, sodium stearyl glutamate, potassium cetyl phosphate;

the emollient includes: at least one of jojoba seed oil, polydimethylsiloxane, hydrogenated polyisobutylene, cyclopentadimethylsiloxane, shea butter, C12-15 alcohol benzoate, isohexadecane, octyl methicone, phenyl trimethicone, dioctyl carbonate, pentaerythritol tetrakis (ethyl hexanoate), coconut oil, squalane, olive oil, meadowfoam seed oil, propyl heptyl caprylate, tri (ethyl hexanoate), caprylic/capric triglyceride, isononyl isononanoate;

the preservative comprises: at least one of methyl hydroxybenzoate, propyl hydroxybenzoate, phenoxyethanol, sodium benzoate, and potassium sorbate;

the antioxidant comprises: at least one of p-hydroxyacetophenone and tocopherol acetate;

the thickener comprises: at least one of hectorite of distearyl dimonium, beeswax, ceresin, stearyl alcohol, cetyl alcohol, cetostearyl alcohol, behenyl alcohol, stearic acid, behenic acid, hectorite, xanthan gum, acacia gum, hydroxyethyl cellulose, acrylic acid (ester)/C10-30 alkanol acrylate crosspolymer, hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, polyacrylate crosspolymer-6, sodium polyacrylate, carbomer, ammonium acryloyldimethyl taurate/VP copolymer;

the chelating agent comprises: disodium EDTA;

the solubilizer comprises: at least one of PEG-60 hydrogenated castor oil, PEG-40 hydrogenated castor oil, PPG-26-Butaneth-26;

the pH regulator comprises: at least one of aminomethyl propanol, arginine, citric acid, sodium citrate, and triethanolamine.