CN115176803A

CN115176803A - Ips typographus attractant and application thereof, attracting core and preparation method and application thereof

Info

Publication number: CN115176803A
Application number: CN202210915918.3A
Authority: CN
Inventors: 于春梅; 韩富忠; 方加兴; 孔祥波; 刘福; 邓梅; 张苏芳; 王晓婷; 孙万桂
Original assignee: Institute Of Forest Ecological Environment And Nature Conservation Chinese Academy Of Forestry Sciences; Qinghai Provincial Forest Disease And Insect Pest Control And Quarantine Station Qinghai Provincial Wildlife Epidemic Disease Monitoring Station
Current assignee: Institute Of Forest Ecological Environment And Nature Conservation Chinese Academy Of Forestry Sciences; Qinghai Provincial Forest Disease And Insect Pest Control And Quarantine Station Qinghai Provincial Wildlife Epidemic Disease Monitoring Station
Priority date: 2022-08-01
Filing date: 2022-08-01
Publication date: 2022-10-14
Anticipated expiration: 2042-08-01
Also published as: CN115176803B

Abstract

The invention provides an ips typographus attractant and application thereof, an attracting core and a preparation method and application thereof, and belongs to the technical field of green prevention and control of ips typographus. The attractant comprises the following components in parts by weight: 10 parts of 2-methyl-3-butene-2-alcohol, 0.02 part of (+) -cis-verbenol, 0.02 part of (+) -trans-verbenol and 4 parts of R- (+) -alpha-pinene. The attractant provided by the invention can attract ips typographus, has the advantages of strong specificity, excellent attracting effect, long lasting period, environmental friendliness and the like, is suitable for monitoring and controlling the population of ips typographus, and is not influenced by topography and topography.

Description

Ips typographus attractant and application thereof, attracting core and preparation method and application thereof

Technical Field

The invention belongs to the technical field of green prevention and control of ips typographus, and particularly relates to an ips typographus attractant and application thereof, an attracting core and a preparation method and application thereof.

Background

Ips typographus l is the most harmful trunk borer in spruce forest, mainly damaging the phloem, causing the host plant to die in a short time. Spruce ips are distributed in Jilin, heilongjiang, inner Mongolia, qinghai and Xinjiang in China, and can damage spruce species such as red bark spruce Picea koraniensis Nakai, fish scale spruce jezoensis var. A large number of researches show that the global temperature rise shortens the development calendar period of ips typographus, increases the annual generation number, obviously expands the population and aggravates the damage to the ecosystem of the spruce forest.

At present, the control of ips typographus pests is mainly dependent on their aggregation pheromones. The ips typographus aggregata pheromone has successfully realized the monitoring and control of the ips typographus, and the aggrecan pheromone component is a binary compound combination of 2-methyl-3-buten-2-ol and (-cis) -verbenol identified by Sweden. A series of attractant products are derived by taking the two components as the core, and play a key role in controlling ips typographus. The ips typographus gathering pheromone attracting core is introduced in China after 2000 years, and at present, although the application of the ips typographus attractant is advanced to a certain extent, the ips typographus attractant is improved on the basis of the two core components.

In the process of the ips typographus invading the host, the invasion behavior is influenced by the host volatile matter and the pheromone generated by the host itself. Ips bark beetles metabolize α -pinene released from host plants into a series of monoterpenoids or ketones, such as S- (-cis-verbenol, (-trans) -verbenone, (+) -cis-verbenol, (+) -trans-verbenol, and (+) -verbenone. However, in the research and application of bark beetle attractant or repellent, only the components of negative isomer are focused, for example, S- (-cis-verbenol is the aggregation pheromone component of various bark beetles such as spruce bark beetle, photobuttock bark beetle Ips nitidus Eggers; (-trans-verbenol is the main component of aggregation pheromone of ips nitdus, dendroctonus johnsoniae Hopkins; the (-verbenone) is an antagonist of ips typographus aggregation pheromone. The (+) -cis-verbenol, (+) -trans-verbenol, (+) -verbenone of the orthoisomer are important metabolites of ips typographus, but the biological functions are not reported. Therefore, for ips typographus, no positive isomer pheromone attractant product is reported at present.

Disclosure of Invention

In view of the above, the invention aims to provide an ips typographus attractant and application thereof, an attracting core and a preparation method and application thereof.

In order to achieve the above object, the present invention provides the following technical solutions:

the invention provides an ips typographus attractant which comprises the following components in parts by weight:

10 parts of 2-methyl-3-butene-2-alcohol, 0.02 part of (+) -cis-verbenol, 0.02 part of (+) -trans-verbenol and 4 parts of R- (+) -alpha-pinene.

The invention also provides the application of the ips typographus attractant in the technical scheme in attracting ips typographus.

The invention also provides a lure core for luring ips typographus, which comprises a slow release carrier, a polyurethane sponge arranged in the slow release carrier and an attractant adsorbed on the polyurethane sponge; the attractant is the attractant in the technical scheme; the slow release carrier is a polyethylene plastic bag.

Preferably, in a single lure:

the content of 2-methyl-3-buten-2-ol was 10g;

the content of (+) -cis-verbenol is 20mg;

the content of (+) -trans-verbenol is 20mg;

the content of R- (+) -alpha-pinene was 4g.

Preferably, the content of the polyurethane sponge in the single lure is 3g.

Preferably, the polyethylene plastic bag has a thickness of 0.2mm, a length of 150mm and a width of 70mm.

The invention also provides a preparation method of the lure according to the technical scheme, which comprises the following steps:

and (3) placing the polyurethane sponge in a slow-release carrier, then dropwise adding the attractant on the polyurethane sponge, and sealing to obtain the lure core.

The invention also provides application of the trapping core in the technical scheme in trapping ips typographus.

Preferably, the application comprises: the lure core is hung in the forest, and the number of the lure core hung in each mu is 3-5.

The invention has the beneficial effects that:

the attractant provided by the invention can attract ips typographus, has the advantages of strong specificity, excellent attracting effect, long lasting period, environmental friendliness and the like, is suitable for monitoring and controlling the population of ips typographus, and is not influenced by topography and topography.

Drawings

FIG. 1 shows the results of the evaluation test of the biological activity of ips typographus in 5-6 months in 2019. Comparative example 1, comparative example 2, comparative example 3, comparative example 4, comparative example 5, comparative example 6, example 1 and comparative example 7 in this order from left to right;

FIG. 2 shows the results of the evaluation test of the dosage change of R- (+) -alpha-pinene in the attractant on the biological activity of ips typographus. Comparative example 8, example 1, comparative example 9 and comparative example 10 were sequentially provided from left to right;

FIG. 3 shows the result of the biological evaluation test of (+) -cis-verbenol in attractant on ips typographus. Comparative example 11, example 1, comparative example 12, comparative example 13 and comparative example 14 in this order from left to right;

FIG. 4 shows the result of the biological evaluation test of (+) -trans-verbenol in attractant on ips typographus. Comparative example 15, example 1, comparative example 16, comparative example 17 and comparative example 18 in this order from left to right;

FIG. 5 shows the evaluation test results of the 2-methyl-3-buten-2-ol dosage change in the attractant on the biological activity of ips typographus. Comparative example 19, comparative example 20, example 1, comparative example 21, comparative example 22 and comparative example 23 in this order from left to right;

FIG. 6 shows the results of the evaluation test of the biological activity of the attractant and the aggregation pheromone attractant of the present invention on ips typographus. Example 1, comparative example 24 and comparative example 25 were provided in this order from left to right.

Detailed Description

The invention provides an attractant for ips typographus, which comprises the following components in parts by weight:

10 parts of 2-methyl-3-butene-2-alcohol, 0.02 part of (+) -cis-verbenol, 0.02 part of (+) -trans-verbenol and 4 parts of R- (+) -alpha-pinene. In the present invention, the source of the reagent is not particularly limited, and a commercially available product may be used. In the present invention, the 2-methyl-3-buten-2-ol (carbofuran, purity > 95%), (+) -cis-verbenol (carbofuran, purity > 95%), (+) -trans-verbenol (carbofuran, purity > 95%), R- (+) -alpha-pinene (avastin, purity > 95%).

In the present invention, the content of the polyurethane sponge in a single lure is preferably 5g. In the present invention, the polyethylene bag is preferably a 20s black polyethylene bag, and comprises 0.4% (W%) antioxidant 168, 0.6% (W%) dioctyl biphenyldicarboxylate, 99% (W%) low density polyethylene, 150mm in length, and 70mm in width. In the present invention, the content of 2-methyl-3-buten-2-ol in a single lure is preferably 10g; the content of (+) -cis-verbenol is preferably 20mg; the content of (+) -trans-verbenol is preferably 20mg; the content of R- (+) -alpha-pinene is preferably 4g. The invention has no special requirements on the sources of polyurethane sponge and polyethylene plastic bags, and only needs to use commercial products.

In the present invention, the preparation method of the lure preferably comprises: dissolving the (+) -cis-verbenol in 2-methyl-3-buten-2-ol in a desired amount, preferably at a concentration of 20 mg/10 g; the (+) -trans-verbenol is dissolved in 2-methyl-3-buten-2-ol in the required amount, preferably in a concentration of 20 mg/10 g; the R- (+) -alpha-pinene is dissolved in 2-methyl-3-buten-2-ol in the required amount, the concentration of which is preferably 4 g/10 g; the 4 components are mixed evenly and then directly dripped into polyurethane sponge in a 20s black polyethylene plastic bag carrier, and the lure is obtained after sealing.

The invention also provides the application of the lure core in the technical scheme in luring ips typographus.

The ips trapping core provided by the invention can be used for trapping and killing a large number of ips, population monitoring of ips, and the like. The ips trapping core provided by the invention is easy to process and produce, has low cost, and 4 components are stably released on the carrier respectively, the lasting period can be maintained at 40-50 days, and the requirement of trapping a large amount of ips in production can be met. The trapping core provided by the invention is an efficient, lasting and environment-friendly trapping core for ips typographus, is applied to the trapping, killing, preventing and controlling of a large number of ips typographus, and has remarkable effect. The ips typographus trapping core has wide industrialization prospect. In addition, the preparation method is simple and is particularly suitable for large-area popularization in production.

In the invention, the lure is preferably hung in a forest land during the application, and the hanging number per mu is preferably 3-5. In a particular embodiment of the invention, the lure is preferably fixed in the middle of the trap and the trap is then suspended from the forest. The trapper is mainly a cross-plate type bark beetle trapper, which is purchased from Beijing Zhongjie Sifang Biotechnology GmbH. The large plate and the small plate are crossed to form a cross shape (the height is 500mm, the width is 300 mm), and the large plate and the small plate are connected with the upper cover through a top hook and connected with the funnel through a bottom hook. The insect collecting bottle is directly connected with the lower edge of the funnel bell mouth. 1 trapping core is hung in the middle position of the cross plate type bark beetle trapper, which is 20cm away from the top end. The trap is hung in a broad, ventilated and sunny area in the forest, and the trap insect collecting bottle is preferably 50cm away from the ground. The traps are spaced at a distance of 50-100 m.

The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.

Example 1

The ips typographus attractant prepared in the embodiment adopts commercially available 2-methyl-3-buten-2-ol, and both the chemical purity and the isomeric purity of the ips typographus attractant are greater than 95%; (+) -cis-verbenol having both chemical and isomeric purity greater than 95%; (+) -trans-verbenol having both chemical and isomeric purity greater than 95%; the chemical purity and the isomeric purity of the R- (+) -alpha-pinene are both more than 95 percent.

Attractant: 10g of 2-methyl-3-buten-2-ol (MB), 20mg of (+) -cis-verbenol, 20mg of (+) -trans-verbenol, and 4g of R- (+) -alpha-pinene.

The (+) -cis-verbenol, trans-verbenol and R- (+) -alpha-pinene are sequentially and directly added into 2-methyl-3-buten-2-ol to prepare the attractant.

Attracting the core: the attractant was added to a polyurethane sponge weighing 5g and placed in a black polyethylene plastic bag of 0.2mm thickness.

The prepared lure is sealed and packaged by aluminum foil paper and is refrigerated at 4 ℃.

Example 1, comparative examples 1-6 were prepared by adding (+) -cis-verbenol, (+) -trans-verbenol and R- (+) - α -pinene directly to 2-methyl-3-buten-2-ol in certain amounts. Comparative example 7 a blank control was made with a trap (made of polyethylene plastic bag and polyurethane sponge only, without any ingredients) from which a blank lure was hung.

The application of the ips typographus attractant lure core prepared in the application example 1 and the comparative examples 1-7 to lure ips typographus comprises the following specific steps:

a) Manufacturing the trap: the trapper is mainly a cross-plate type bark beetle trapper, which is purchased from Beijing Zhongjie Sifang Biotechnology GmbH. The large plate and the small plate are crossed to form a cross shape (the height is 500mm, the width is 300 mm), and the large plate and the small plate are connected with the upper cover through the hook at the top and connected with the funnel through the hook at the bottom. The insect collecting bottle is directly connected with the bell mouth at the lower edge of the funnel.

b) Suspending the lure: the middle position of the cross plate type bark beetle trap, which is 20cm away from the top end, is hung with 1 lure.

c) The field setting of trapper: 3 replicates were set for each test treatment, traps were placed at the edge of the spruce forest and in open, sunny, ventilated areas, the intra-block trap spacing was approximately 30m, and the inter-block trap spacing was approximately 60m. Two 2m long bamboo poles with the diameter of 5cm are inserted into the soil layer at the depth of about 50cm, the distance between the two bamboo poles is about 35cm, then the trap is fixed between the two bamboo poles, and the height of the trap insect collecting bottle above the ground is about 50cm.

d) Data survey and trap emptying: 3 replicates were set for each treatment and the number of beetles trapped per treatment was the average of 3 traps. Observing the number of the bark beetles in the bark beetle collecting bottle once every 2 to 3 days according to weather conditions, placing the trapped bark beetles in a plastic box after counting, bringing the bark beetles out of forest, and carrying out centralized killing treatment.

The application of the composition is used for attracting ips typographus according to the specific using steps. The result of the blank control of the trap (comparative example 7) with the blank lure hung in 2019 from 17 th month 5 to 17 th month 6 is shown in figure 1. The distance between the traps is 30m, the traps are hung between 2 bamboo poles, and the distance between the trap bottles and the ground is about 50cm. The experiment was set up for 3 replicates per treatment, and the number of beetles trapped per treatment was the average of 3 traps. Observing the bark beetle number in the bark beetle collecting bottle every 2-3 days according to the weather condition.

Comparative example 1

The attractant is as follows: 10g of 2-methyl-3-buten-2-ol (MB), 20mg of (+) -cis-verbenol; the rest of the procedure was the same as in example 1, and the results are shown in FIG. 1.

Comparative example 2

The attractant is 10g of 2-methyl-3-butylene-2-alcohol (MB) and 20mg of (+) -trans-verbenol; the rest of the procedure was the same as in example 1, and the results are shown in FIG. 1.

Comparative example 3

The attractant is 1g of 2-methyl-3-butene-2-ol (MB), 4g of R- (+) -alpha-pinene; the rest of the procedure was the same as in example 1, and the results are shown in FIG. 1.

Comparative example 4

The attractant is 10g of 2-methyl-3-butene-2-ol (MB), 20mg of (+) -cis-verbena enol and 4g of R- (+) -alpha-pinene; the rest of the procedure was the same as in example 1, and the results are shown in FIG. 1.

Comparative example 5

The attractant is 10g of 2-methyl-3-butene-2-alcohol (MB), 20mg of (+) -trans-verbena enol and 4g of R- (+) -alpha-pinene; the rest of the procedure was the same as in example 1, and the results are shown in FIG. 1.

Comparative example 6

The attractant is 10g of 2-methyl-3-butene-2-ol (MB), 20mg of (+) -cis-verbenol and 20mg of (+) -trans-verbenol; the rest of the procedure was the same as in example 1, and the results are shown in FIG. 1.

Comparative example 7

No attractant but only a lure.

As can be seen from the insect-attracting effect analysis in fig. 1, the ips typographus attractant (example 1) has a very good attracting effect and the attracting amount is the largest. Compared with example 1, the attracting effect of the ips typographus attractant (comparative examples 1, 2, 4, 5 and 6) on ips typographus is poor. The control blanks of comparative example 3 and comparative example 7 had no attractant activity for ips typographus. The experiment shows that the mixture of the four substances of the 2-methyl-3-buten-2-ol, (+) -cis verbenol, (+) -trans verbenol and R- (+) -alpha-pinene has good attraction effect on ips typographus. The ips typographus attractant prepared in example 1 can be used for comprehensively controlling the damage of ips typographus in forests. The experimental conclusion confirms that only the mixture of the four substances of 2-methyl-3-buten-2-ol, (+) -cis verbenol, (+) -trans verbenol and R- (+) -alpha-pinene has very high biological activity and has great market application value, and the biological activity of the other two-component combination or the three-component combination is poor.

Comparative example 8

The attractant is as follows: 10g of 2-methyl-3-buten-2-ol (MB), 20mg of (+) -cis-verbenol, 20mg of (+) -trans-verbenol, 2g of R- (+) -alpha-pinene; the rest of the procedure was the same as in example 1, and the results are shown in FIG. 2.

Comparative example 9

The attractant is as follows: 10g of 2-methyl-3-buten-2-ol (MB), 20mg of (+) -cis-verbenol, 20mg of (+) -trans-verbenol, and 8g of R- (+) -alpha-pinene; the rest of the procedure was the same as in example 1, and the results are shown in FIG. 2.

Comparative example 10

No attractant, only a lure.

The woodland biological activity test of the spruce bark beetle treated in example 1 and comparative examples 8-10 was carried out in 2019 on the days 6, 10 and 7, 10 according to the specific implementation procedure in example 1. The specific results are shown in FIG. 2. The experiment was repeated 3 times per treatment setting, and the number of beetles trapped per treatment was the average of 3 traps. Observing the bark beetle number in the bark beetle collecting bottle every 2-3 days according to the weather condition. The collected bark beetles are brought out of the woodland and are intensively killed.

From the analysis of the insect-attracting effect in fig. 2, it can be seen that the number of the attracting ips typographus increases with the increase of the content of R- (+) - α -pinene. When the addition amount of the R- (+) -alpha-pinene is 4g and 8g, the trapping amount is much larger than 2g, and the trapping amount is the highest under 4g. In the practical application, the dosage of 4g is selected as the best in terms of the attracting effect of the attractant in the core-attracting forest, the lasting period and the economic use.

Comparative example 11

The attractant is as follows: 10g of 2-methyl-3-buten-2-ol (MB), 10mg of (+) -cis-verbenol, 20mg of (+) -trans-verbenol, and 4g of R- (+) -alpha-pinene; the rest of the procedure was the same as in example 1, and the results are shown in FIG. 3.

Comparative example 12

The attractant is as follows: 10g of 2-methyl-3-buten-2-ol (MB), 30mg of (+) -cis-verbenol, 20mg of (+) -trans-verbenol, and 4g of R- (+) -alpha-pinene; the rest of the procedure was the same as in example 1, and the results are shown in FIG. 3.

Comparative example 13

The attractant is as follows: 10g of 2-methyl-3-buten-2-ol (MB), 40mg of (+) -cis-verbenol, 20mg of (+) -trans-verbenol, and 4g of R- (+) -alpha-pinene; the rest of the procedure was the same as in example 1, and the results are shown in FIG. 3.

Comparative example 14

No attractant but only a lure.

The woodland biological activity test of the treatment of example 1 and comparative examples 11-14 was carried out on 6 months to 10 months and 10 days in 2019 according to the specific implementation procedure in example 1, and the specific results are shown in FIG. 3. The experiment was performed in 3 replicates per treatment setting, and the number of bark beetles trapped per treatment was the average of 3 traps. Observing the bark beetle number in the bark beetle collecting bottle every 2-3 days according to the weather condition. The collected bark beetles are intensively taken out of the woodland and destroyed.

From the analysis of the insect-attracting effect in fig. 3, under the condition that 10g of 2-methyl-3-butene-2-ol (MB), 20mg of (+) -trans-verbenol and 4g of R- (+) -alpha-pinene are fixed, the (+) -cis-verbenol shows the insect-attracting activity under the conditions of 10mg, 20mg, 30mg and 40mg of 4 testing doses. The biological activity difference is obvious under the test doses of 20mg, 30mg and 40mg compared with the 10mg dose, the biological activity difference is not obvious between 20mg and 40mg, and the trapping amount is higher under the 20mg dose. From the perspective of attractant core-luring and intercropping effects, lasting period and economic use, the optimal use dosage range of (+) -cis-verbenol is 20 mg-40 mg, and the optimal dosage is 20mg.

Comparative example 15

The attractant is as follows: 10g of 2-methyl-3-buten-2-ol (MB), 20mg of (+) -cis-verbenol, 10mg of (+) -trans-verbenol, and 4g of R- (+) -alpha-pinene; the rest of the procedure was the same as in example 1, and the results are shown in FIG. 4.

Comparative example 16

The attractant is as follows: 10g of 2-methyl-3-buten-2-ol (MB), 20mg of (+) -cis-verbenol, (+) -trans-verbenol 30mg, 4g of R- (+) -alpha-pinene; the rest of the procedure was the same as in example 1, and the results are shown in FIG. 4.

Comparative example 17

The attractant is as follows: 10g of 2-methyl-3-buten-2-ol (MB), 20mg of (+) -cis-verbenol, 40mg of (+) -trans-verbenol, 4g of R- (+) -alpha-pinene; the rest of the procedure was the same as in example 1, and the results are shown in FIG. 4.

Comparative example 18

No attractant, only a lure.

The specific implementation steps in example 1 are followed, and the forest biological activity test of the treatment of example 1 and comparative examples 15-18 is carried out in 2019 on 6, 10-7, 10 days, and the specific results are shown in FIG. 4. The experiment was performed in 3 replicates per treatment setting, and the number of bark beetles trapped per treatment was the average of 3 traps. Observing the bark beetle number in the bark beetle collecting bottle every 2-3 days according to the weather condition. The collected bark beetles are brought out of the woodland and are intensively killed.

From the analysis of the insect-attracting effect in fig. 4, it can be seen that, in the case where the test doses of 2-methyl-3-buten-2-ol (MB) 10g, (+) -trans-verbenol 20mg and R- (+) - α -pinene 4g were fixed, the differences in biological activities of (+) -trans-verbenol at 20mg, 30mg and 40mg of the 3 test doses were not significant, but were all significantly higher than the amount trapped at the 10mg dose, and the amount trapped at the 20mg dose was the highest. From the perspective of attractant core-luring and intercropping effects, duration and economic use of the attractant, the optimal dosage range of the (+) -trans-verbenol should be between 20mg and 40mg, and the optimal dosage is 20mg.

Comparative example 19

The attractant is as follows: 1g of 2-methyl-3-buten-2-ol (MB), 20mg of (+) -cis-verbenol, 20mg of (+) -trans-verbenol, and 4g of R- (+) -alpha-pinene; the rest of the procedure was the same as in example 1, and the results are shown in FIG. 5.

Comparative example 20

The attractant is as follows: 5g of 2-methyl-3-buten-2-ol (MB), 20mg of (+) -cis-verbenol, 20mg of (+) -trans-verbenol, and 4g of R- (+) -alpha-pinene; the rest of the procedure was the same as in example 1, and the results are shown in FIG. 5.

Comparative example 21

The attractant is as follows: 15g of 2-methyl-3-buten-2-ol (MB), 20mg of (+) -cis-verbenol, 20mg of (+) -trans-verbenol, and 4g of R- (+) -alpha-pinene; the rest of the procedure was the same as in example 1, and the results are shown in FIG. 5.

Comparative example 22

The attractant is as follows: 20g of 2-methyl-3-buten-2-ol (MB), 20mg of (+) -cis-verbenol, 20mg of (+) -trans-verbenol, and 4g of R- (+) -alpha-pinene; the rest of the procedure was the same as in example 1, and the results are shown in FIG. 5.

Comparative example 23

No attractant, only a lure.

The woodland biological activity test of the treatment of example 1 and comparative examples 19-23 was carried out on 6 months 15 to 7 months 15 days 2019 according to the specific implementation procedure of example 1, and the specific results are shown in FIG. 5. The experiment was performed in 3 replicates per treatment setting, and the number of bark beetles trapped per treatment was the average of 3 traps. Observing the number of the bark beetles in the bark beetle collecting bottle every 2-3 days according to weather conditions, putting the collected bark beetles into a plastic box to be taken out of the forest land after counting, and carrying out centralized killing treatment.

From the insect attractant effect analysis of fig. 5, it can be seen that 2-methyl-3-buten-2-ol (MB) was tested at 5 test doses of 1g, 5g, 10g, 15g and 20g, on the basis that the test doses of (+) -cis-verbenol 20mg, (+) -trans-verbenol 20mg and R- (+) - α -pinene 4g were unchanged. With the addition of 2-methyl-3-buten-2-ol (MB), the trapping activity on ips typographus is increased. At doses of 10g, 15g and 20g, the differences in trap numbers were not significant, with the 10g dose being the best, significantly higher than the 1g and 5g doses. From the viewpoints of attractant core-luring and intercropping effects, duration and economic use, the recommended optimal dosage range of the 2-methyl-3-butene-2-ol is 10 g-15 g, and the optimal dosage is 10g.

Comparative example 24

The attractant is as follows: 10g of 2-methyl-3-buten-2-ol (MB), 40mg of S- (-cis-verbenol; the rest of the results are shown in FIG. 6, as in example 1.

Comparative example 25

No attractant but only a lure.

The specific results of the woodland bioactivity test of the treatment of example 1 and comparative examples 24 and 25 were carried out in 2019 on days 6, 15 to 7, 15 according to the specific implementation steps in example 1, and are shown in fig. 6. The experiment was performed in 3 replicates per treatment setting, and the number of bark beetles trapped per treatment was the average of 3 traps. Observing the number of the bark beetles in the bark beetle collecting bottle every 2-3 days according to weather conditions, taking the collected bark beetles out of the forest land after counting, and carrying out centralized killing treatment.

As can be seen from the analysis of the trapping effect in FIG. 6, the two-element composition attractant of aggregation pheromone composed of 10g of 2-methyl-3-buten-2-ol (MB), 40mg of S- (-cis-verbenol (S) -, and 10g of 2-methyl-3-buten-2-ol (MB), 20mg of (+) -cis-verbenol (S) - (+) -trans-verbenol (S) - (+) -cis-verbenol (S) -) and 4g of R- (+) -alpha-pinene (R) - (+) -alpha-pinene) all had good trapping effect on ips typographus spruce. However, the trapping activity of the composition consisting of 10g of 2-methyl-3-buten-2-ol (MB), 20mg of (+) -cis-verbenol, 20mg of (+) -trans-verbenol and 4g of R- (+) -alpha-pinene on ips typographus is better than that of the commonly used aggregation pheromone core trapping effect, and the trapping effect is improved by 2 times. The attractant can be used as a product for trapping ips typographus in a large quantity.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. The ips typographus attractant is characterized by comprising the following components in parts by weight:

10 parts of 2-methyl-3-butene-2-ol, 0.02 part of (+) -cis-verbenol, 0.02 part of (+) -trans-verbenol and 4 parts of R- (+) -alpha-pinene.

2. The use of the ips typographus attractant of claim 1 or 1 for attracting ips typographus.

3. A lure core for luring ips typographus is characterized by comprising a slow release carrier, a polyurethane sponge arranged in the slow release carrier and an attractant adsorbed on the polyurethane sponge; the attractant is the attractant of claim 1; the slow release carrier is a polyethylene plastic bag.

4. A lure according to claim 3, wherein the amount of 2-methyl-3-buten-2-ol in a single lure is 10g;

the content of (+) -cis-verbenol is 20mg;

the content of (+) -trans-verbenol is 20mg;

the content of R- (+) -alpha-pinene was 4g.

5. A lure according to claim 3, wherein the polyurethane sponge content of a single lure is 5g.

6. The lure according to claim 3, wherein the polyethylene plastic bag has a thickness of 0.2mm, a length of 150mm and a width of 70mm.

7. A method of making a lure according to any one of claims 3 to 6, characterised by the steps of:

8. The use of an attractant core as claimed in any one of claims 3 to 6 for attracting ips typographus.

9. The application according to claim 8, wherein the application comprises: the lure core is hung in the forest, and the number of the lure core hung in each mu is 3-5.