CN115161192A - Automatic sampling device and air microorganism sampling method - Google Patents
Automatic sampling device and air microorganism sampling method Download PDFInfo
- Publication number
- CN115161192A CN115161192A CN202211086438.7A CN202211086438A CN115161192A CN 115161192 A CN115161192 A CN 115161192A CN 202211086438 A CN202211086438 A CN 202211086438A CN 115161192 A CN115161192 A CN 115161192A
- Authority
- CN
- China
- Prior art keywords
- sampling
- magnetic rod
- pipe
- sleeve
- air
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/06—Magnetic means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
Abstract
The invention discloses an automatic sampling device and an air microorganism sampling method, and the automatic sampling device comprises sampling pipes, magnetic rod sleeves, magnetic rods, a sampling head and a positioning disk, wherein the positioning disk is rotationally connected to a supporting shaft and driven by a rotary driving device to rotate; the magnetic rod is driven to lift by the first lifting structure, a magnetic rod sleeve locking device is arranged on one side of the top of the magnetic rod, and after the magnetic rod extends into the magnetic rod sleeve, the magnetic rod sleeve can be locked on the magnetic rod through the magnetic rod sleeve locking device; the sampling head is driven to lift by the second lifting structure, an air exhaust channel and an air inlet channel are arranged in the sampling head, the air exhaust channel is connected with an air exhaust device, and the air exhaust channel and the air inlet channel respectively correspond to an air outlet and an air inlet of the sampling tube. The invention can automatically complete the collection and nucleic acid extraction of air microorganisms, avoids the mixed bacteria pollution caused by complicated operation, and is beneficial to quickly complete the detection of the air microorganisms.
Description
Technical Field
The invention relates to the technical field of microorganism detection, in particular to an automatic sampling device and an air microorganism sampling method.
Background
Microorganisms in human, animal and plant bodies and soil can be dispersed in the air by droplets, dust, and the like, so that the air contains a certain kind and number of microorganisms. There are generally no pathogenic microorganisms in the air theoretically, but aerosols of pathogenic microorganisms are often suspended in the air near hospitals, veterinary hospitals and livestock houses, and healthy people or animals are often infected by inhalation. Air contaminated by pathogenic microorganisms can often be a source or vehicle of contamination, causing epidemic disease. Therefore, the detection of airborne microorganisms is of great significance for the prevention and control of infectious diseases and the hygiene supervision and protection of the environment.
The air sampler is an important tool in the air microorganism detection process, and has a variety of types, wherein the impact air sampler collects microorganism particles in air in a sampling liquid in a jet air flow mode. In the sampling process, after the sampling liquid is added into the sampler, air suction power is started, air enters from an air inlet of the sampler, microbial particles in the air impact the sampling liquid of the sampler, and the microbial particles are captured due to the adhesion of the liquid.
Before detecting air microorganism, need extract the nucleic acid of microorganism in advance, current air microorganism gathers and nucleic acid extraction mainly relies on manual operation, and air microorganism sample gathers and nucleic acid extraction needs the different instrument and equipment of manual operation to accomplish, complex operation, and it is long to consume time, and technical requirement is high.
Disclosure of Invention
The invention aims to provide an automatic sampling device and an air microorganism sampling method, which are used for solving the problems in the prior art, can automatically complete the collection and nucleic acid extraction of air microorganisms, do not need manual operation, have low technical requirements, avoid the pollution of mixed bacteria caused by complicated operation, are convenient and quick, are favorable for quickly completing the detection of the air microorganisms and quicken the detection process of the air microorganisms.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides an automatic sampling device which comprises sampling tubes, magnetic rod sleeves, magnetic rods, a sampling head and a positioning disc, wherein the positioning disc is rotatably connected to a supporting shaft and driven to rotate by a rotary driving device; the magnetic rod is positioned above the sampling pipes and driven to lift by a first lifting structure, a magnetic rod sleeve locking device connected to the first lifting structure is arranged on one side of the top of the magnetic rod, the magnetic rod and the magnetic rod sleeve locking device can be driven to lift synchronously by the first lifting structure, each sampling pipe can rotate to a position right below the magnetic rod, and after the magnetic rod descends and extends into the opposite magnetic rod sleeve, the magnetic rod sleeve can be locked on the magnetic rod by the magnetic rod sleeve locking device; the sampling head is located the sampling pipe top to by the drive of second elevation structure lift, be equipped with bleed passage and inlet channel in the sampling head, bleed passage upper end is connected air exhaust device, each the sampling pipe homoenergetic rotates to under the sampling head, bleed passage with the inlet channel lower extreme respectively with just right the gas outlet and the air inlet at sampling pipe top are corresponding.
Preferably, the support shaft is rotatably connected with a support ring through a bearing, the lower end of the support ring is fixedly connected to the positioning disc, a plurality of longitudinal sliding grooves are formed in the peripheral surface of the support ring, the lower ends of the sliding grooves are respectively provided with a fixing clamping groove, each sampling pipe is uniformly distributed, and the sampling pipes are adjacent to each other and fixedly connected to each other to form a sampling pipe ring, the sampling pipe ring is sleeved on the support ring, fixing buckles corresponding to the sliding grooves are arranged on the inner side surface of the sampling pipe ring respectively, and the fixing buckles slide into the sliding grooves to be clamped and fixed to the sampling pipe ring in the fixing clamping grooves.
Preferably, a damping shaft is fixedly arranged on a bulge at the top end of the supporting shaft, damping bulges are arranged on two opposite sides of the periphery of the damping shaft, and the damping bulges are fixed on the damping shaft and supported at the upper end of the supporting ring.
Preferably, the periphery of the positioning plate is provided with deep grooves and shallow grooves which are arranged in a crossed manner and have the same number, and the deep grooves and the shallow grooves are uniformly distributed on the periphery of the positioning plate; the rotary driving device comprises a stirring piece and a rotary driving motor for driving the stirring piece to rotate, the stirring piece comprises a shallow groove stirring piece, a deep groove stirring piece and a connecting disc, the shallow groove stirring piece and the deep groove stirring piece are respectively connected to two ends of the connecting disc, the shallow groove stirring piece is matched with the shallow groove, the deep groove stirring piece is matched with the deep groove, when the stirring piece stirs the positioning disc, the deep groove stirring piece enters the deep groove, the shallow groove stirring piece enters the shallow groove, the positioning disc rotates one distance between the shallow groove and the deep groove every time the stirring piece rotates one circle, the number of sampling tubes is equal to the number of the shallow groove or the deep groove, and each sampling tube corresponds to one shallow groove and one deep groove.
Preferably, the shallow slot shifting member is a sector cylinder, the shallow slot is an arc-shaped slot with the radius equal to that of the sector cylinder, the deep slot shifting member is a cylinder, and the width of the deep slot is equal to the diameter of the cylinder.
Preferably, the upper end of the sampling pipe is provided with an air inlet pipe and an air outlet pipe, the pipe orifice at the top of the air inlet pipe is an air inlet of the sampling pipe, the pipe orifice at the top of the air outlet pipe is an air outlet of the sampling pipe, the lower end inside the sampling pipe is provided with an inclined plane opposite to the air inlet pipe, and the lower end of the air inlet pipe extends into the sampling pipe to the position above the inclined plane; the bar magnet sleeve pipe is including the sleeve pipe handle, spacing ring, sleeve pipe pole portion and the sleeve pipe head portion that from top to bottom connect gradually, the sleeve pipe handle top is equipped with the casing mouth, through the casing mouth can with the bar magnet inserts in the bar magnet sleeve, the spacing ring with the department of meeting periphery of sleeve pipe handle is equipped with the sampling tube lid, sampling tube lid lower extreme is equipped with the sealing portion, through the outlet duct can with the sleeve pipe head portion sleeve pipe pole portion with the spacing ring inserts in the sampling tube, make the sleeve pipe head stretch into to bottom in the sampling tube, and can pass through the spacing ring with the outlet duct cooperation is connected and is realized sealedly, works as the spacing ring with the outlet duct cooperation is connected when realizing sealedly, the sealing portion shutoff simultaneously the intake pipe top.
Preferably, be close to on the sleeve handle one side of sealing part is equipped with the casing groove, bar magnet sleeve pipe locking device includes the electromagnetic lock, the electromagnetic lock is fixed in bar magnet top one side go up and down to construct, it is protruding to be equipped with the lock on the electromagnetic lock, through the electromagnetic lock can be controlled the protruding salient of lock in the electromagnetic lock or retraction in the electromagnetic lock, the bar magnet descends and stretches into just right the back in the bar magnet casing, the protruding salient of lock electromagnetic lock and card go into the casing groove, will the bar magnet sleeve pipe lock in on the bar magnet.
Preferably, the sealing includes sealing cylinder and inverted cone, sealing cylinder upper end fixed connection be in the sampling tube cap lower extreme, sealing cylinder lower extreme with the bottom surface of inverted cone is connected, sealing cylinder's diameter with the bottom surface diameter of inverted cone equals, and with the internal diameter of intake pipe equals, works as the spacing ring inserts when realizing sealedly in the outlet duct, the inverted cone with sealing cylinder inserts the shutoff in the intake pipe top.
Preferably, the outer diameters of the limiting ring, the sleeve rod part and the sleeve head part are sequentially reduced, the wall thicknesses of the limiting ring, the sleeve rod part and the sleeve head part are equal, and the sleeve handle, the limiting ring, the sleeve rod part and the sleeve head part are integrally formed; the inner diameter of the air inlet pipe is smaller than that of the air outlet pipe.
The invention also provides an air microorganism sampling method, which adopts the automatic sampling device to sample and comprises the following steps:
s1: the magnetic rod moves downwards into the magnetic rod sleeve, the magnetic rod sleeve is locked on the magnetic rod, and then the magnetic rod moves upwards to drive the magnetic rod sleeve to move out of the sampling tube, so that an air outlet and an air inlet of the sampling tube are exposed;
s2: the positioning disc rotates to enable the sampling pipe with the exposed air outlet and the exposed air inlet to rotate to be right below the sampling head, the sampling head descends to enable the air exhaust channel and the air inlet channel to be communicated with the air outlet and the air inlet of the sampling pipe respectively, and the air exhaust device is started to perform air sampling;
s3: after sampling is finished, the sampling head rises, and the positioning disc rotates the sampling pipe after sampling to be right below the magnetic rod;
s4: the magnetic rod moves downwards to drive the magnetic rod sleeve to extend into the sampling tube, so that the magnetic beads, which adsorb the air microorganism nucleic acid sample in the sampling tube, are adsorbed on the outer wall of the magnetic rod sleeve;
s5: the magnetic rod moves upwards to drive the magnetic rod sleeve to move out of the sampling tube, and the magnetic beads adsorbed with the air microorganism nucleic acid samples are eluted.
Compared with the prior art, the invention has the following technical effects:
the automatic sampling device and the air microorganism sampling method provided by the invention have the advantages that the first lifting structure can drive the magnetic rod and the magnetic rod sleeve locking device to synchronously lift, the magnetic rod sleeve can be locked on the magnetic rod or unlocked with the magnetic rod through the magnetic rod sleeve locking device, so that the magnetic rod sleeve can be conveniently drawn out of the sampling pipe or stretched into the sampling pipe, the second lifting structure can drive the sampling head to lift, so that the sampling head is matched with the sampling pipe to sample air microorganisms, the rotary driving device can drive the positioning disc to rotate, so that the sampling pipe is conveniently positioned, so that the sampling pipes are conveniently sampled for multiple times, the collection and the nucleic acid extraction of the air microorganisms of the device are realized through the control of related devices, the manual operation is not needed, the technical requirement is low, the pollution caused by complicated operation is avoided, the convenience and the rapidness are realized, the detection of the air microorganisms is convenient and the detection process of the air microorganisms is accelerated.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
Fig. 1 is a schematic perspective view of an automated sampling device provided in the present invention;
FIG. 2 is a sectional view of a connection structure of a sampling tube ring and a positioning plate according to the present invention;
FIG. 3 is a perspective view of the connection structure of the positioning plate, the rotary driving device and the support ring according to the present invention;
FIG. 4 is a cross-sectional view of a sampling tube ring of the present invention;
FIG. 5 is a partial cross-sectional view of a connection structure of a sampling head and a second elevation structure according to the present invention;
FIG. 6 is a perspective view of the first lifting structure, the magnetic rod and the connecting structure of the magnetic rod sleeve locking device according to the present invention;
FIG. 7 is an enlarged partial view of portion A of FIG. 6;
FIG. 8 is a perspective view of the combination of the magnetic rod sleeve and the sampling tube of the present invention;
FIG. 9 is a sectional view of the combination of the magnetic rod sheath and the sampling tube of FIG. 8;
FIG. 10 is a cross-sectional view of a sampling tube according to the present invention;
FIG. 11 is a schematic perspective view of the magnetic rod sleeve according to the present invention;
FIG. 12 is a schematic front view of the magnetic rod sleeve of FIG. 11;
FIG. 13 is a schematic diagram showing the comparison of the air flow in the sampling tube of the present invention with that in the conventional sampling tube during air sampling;
in the figure: 100-automatic sampling device, 1-sampling tube, 2-magnetic rod sleeve, 3-magnetic rod, 4-sampling head, 5-positioning disk, 6-supporting shaft, 7-rotary driving device, 8-first lifting structure, 9-magnetic rod sleeve locking device, 10-second lifting structure, 11-air pumping channel, 12-air inlet channel, 13-air outlet, 14-air inlet, 15-bearing, 16-supporting ring, 17-sliding groove, 18-fixed clamping groove, 19-sampling tube ring, 20-fixed buckle, 21-protrusion, 22-damping shaft, 23-damping protrusion, 24-deep groove, 25-shallow groove, 26-toggle piece, 27-rotary driving motor, 28-shallow groove toggle piece, 29-deep groove toggle piece, 30-connecting disk, 31-air inlet tube, 32-air outlet tube, 33-inclined plane, 34-sleeve handle, 35-limiting ring, 36-sleeve, 37-sleeve head, 38-sleeve opening, 39-sampling tube cover, 40-sealing part, 41-sleeve locking part, 41-locking groove, 42-cylindrical rod part, and 45-conical rod part.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention aims to provide an automatic sampling device and an air microorganism sampling method, which are used for solving the problems in the prior art, can automatically complete the collection and nucleic acid extraction of air microorganisms, do not need manual operation, have low technical requirements, avoid the pollution of sundry bacteria caused by complicated operation, are convenient and quick, are beneficial to quickly completing the detection of the air microorganisms and quickening the detection process of the air microorganisms.
In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description thereof.
As shown in fig. 1 to 13, the present embodiment provides an automatic sampling device 100, which includes a sampling tube 1, a magnetic rod sleeve 2, a magnetic rod 3, a sampling head 4, and a positioning disk 5, wherein the positioning disk 5 is rotatably connected to a support shaft 6 and is driven to rotate by a rotation driving device 7, a plurality of sampling tubes 1 are arranged on the positioning disk 5 along a circumferential direction, and one magnetic rod sleeve 2 is nested in each sampling tube 1; the magnetic rod 3 is positioned above the sampling pipes 1 and driven to lift by the first lifting structure 8, one side of the top of the magnetic rod 3 is provided with a magnetic rod sleeve locking device 9 connected to the first lifting structure 8, the magnetic rod 3 and the magnetic rod sleeve locking device 9 can be driven to lift synchronously by the first lifting structure 8, each sampling pipe 1 can rotate to a position right below the magnetic rod 3, and after the magnetic rod 3 descends and extends into the opposite magnetic rod sleeve 2, the magnetic rod sleeve 2 can be locked on the magnetic rod 3 by the magnetic rod sleeve locking device 9; sampling head 4 is located sampling pipe 1 top to by the drive of second elevation structure 10 lift, be equipped with bleed passage 11 and inlet channel 12 in the sampling head 4, bleed device is connected to bleed passage 11 upper end, each sampling pipe 1 homoenergetic rotates to sampling head 4 under, bleed passage 11 and inlet channel 12 lower extreme respectively with just corresponding sampling pipe 1 top gas outlet 13 and air inlet 14 corresponding.
When the sampling device is used, the magnetic rod 3 is driven to descend by the first lifting structure 8 and extend into the magnetic rod sleeve 2, the magnetic rod sleeve 2 is locked on the magnetic rod 3 by the magnetic rod sleeve locking device 9, then the magnetic rod 3 is driven to ascend by the first lifting structure 8, the magnetic rod sleeve 2 is moved out of the sampling tube 1, the air outlet 13 and the air inlet 14 at the top of the sampling tube 1 are exposed, then the positioning disc 5 is driven to rotate by the rotation driving device 7, the sampling tube 1 with the air port 13 and the air inlet 14 exposed is rotated to be under the sampling head 4, the sampling head 4 is driven to descend by the second lifting structure 10, the air exhaust channel 11 and the air inlet channel 12 are respectively communicated with the air outlet 13 and the air inlet 14 of the sampling tube 1, then the air exhaust device is started to sample air, wherein the vacuum pump can be selected as the air exhaust device, and the upper end of the air exhaust channel 11 is connected with the vacuum pump by a pipeline; after sampling is finished, the sampling head 4 is driven to ascend through the second lifting structure 10, the positioning disc 5 is driven to rotate through the rotation driving device 7, and the sampling pipe 1 after sampling is rotated to be right below the magnetic rod 3; the magnetic rod 3 is driven to move downwards through the first lifting structure 8, the magnetic rod sleeve 2 is extended into the sampling tube 1, and magnetic beads which are used for adsorbing air microorganism nucleic acid samples in the sampling tube 1 are adsorbed to the outer wall of the magnetic rod sleeve 2; then, the magnetic rod 3 is driven to move upwards through the first lifting structure 8, the magnetic rod sleeve 2 is removed from the sampling tube 1, and then the magnetic beads with the air microorganism nucleic acid samples are eluted. The collection of this device air microorganism all realizes through controlling of relevant device with nucleic acid extraction, need not manual operation, and technical requirement is low, has avoided the miscellaneous fungus pollution that loaded down with trivial details operation introduced, and convenient and fast is favorable to accomplishing air microorganism's detection fast for air microorganism's detection process. In the device, the rotary driving device 7, the first lifting structure 8, the magnetic rod sleeve locking device 9, the second lifting structure 10 and the air extracting device are all connected with a human-computer interaction terminal through a central controller, and automatic collection and nucleic acid extraction of air microorganisms by the device can be realized through the terminal. The used components such as the sampling tube 1, the magnetic rod sleeve 2 and the like of the device are provided with a plurality of components, so that the frequency of manually replacing consumables is reduced, and the system can realize multiple times of air sampling and nucleic acid extraction without manual intervention. The first lifting structure 8 and the second lifting structure 10 may be implemented by conventional lifting driving devices, which are not described herein.
In this embodiment, the support shaft 6 is rotatably connected with a support ring 16 through a bearing 15, the lower end of the support ring 16 is fixedly connected to the positioning plate 5, a plurality of longitudinal sliding grooves 17 are formed in the outer peripheral surface of the support ring 16, the lower end of each sliding groove 17 is provided with a fixing clamping groove 18, each sampling tube 1 is uniformly distributed and is fixedly connected with the adjacent sliding groove to form a sampling tube ring 19, the sampling tube ring 19 is sleeved on the support ring 16, fixing buckles 20 corresponding to the sliding grooves 17 are respectively arranged on the inner side surface of the sampling tube ring 19, and the fixing buckles 20 slide into the fixing clamping grooves 18 along the sliding grooves 17 to clamp and fix the sampling tube ring 19 on the support ring 16. Wherein, each vertical spout 17 evenly distributed is on the support ring 16 outer peripheral face, is convenient for be fixed in the support ring 16 with the 19 joint of sampling tube ring through fixed buckle 20 on, is convenient for carry out the dismouting. The magnetic rod 3 and the sampling head 4 are respectively positioned above two opposite sides of the sampling pipe ring 19, the sampling head 4 descends to the sampling pipe opening to be combined with the sampling pipe 1 in a closed mode, and the lower end of the sampling head 4 can be provided with a rubber pad to enable the combination to be more closed.
In this embodiment, the protrusion 21 at the top end of the support shaft 6 is fixedly provided with a damping shaft 22, two opposite sides of the periphery of the damping shaft 22 are provided with damping protrusions 23, and the damping protrusions 23 are fixed on the damping shaft 22 and supported at the upper end of the support ring 16, so as to stabilize the rotation of the support ring 16 and the positioning plate 5.
In this embodiment, the periphery of the positioning plate 5 is provided with the deep grooves 24 and the shallow grooves 25 which are arranged in a crossed manner and have the same number, and each deep groove 24 and each shallow groove 25 are uniformly distributed on the periphery of the positioning plate 5; the rotary driving device 7 comprises a toggle piece 26 and a rotary driving motor 27 for driving the toggle piece 26 to rotate, the toggle piece 26 comprises a shallow slot toggle piece 28, a deep slot toggle piece 29 and a connecting disc 30, the shallow slot toggle piece 28 and the deep slot toggle piece 29 are respectively connected to two ends of the connecting disc, the shallow slot toggle piece 28 is matched with a shallow slot 25, the deep slot toggle piece 29 is matched with a deep slot 24, when the toggle piece 26 toggles the positioning disc 5, the deep slot toggle piece 29 enters the deep slot 24, the shallow slot toggle piece 28 enters the shallow slot 25, each toggle piece 26 rotates for one circle, the positioning disc 5 rotates for the distance of one shallow slot 25 and one deep slot 24, the number of sampling tubes 1 is equal to the number of the shallow slots 25 or the deep slots 24, and each sampling tube 1 respectively corresponds to one shallow slot 25 and one deep slot 24. Stir piece 26 through the drive of rotation driving motor 27 and rotate, stir piece 26 and drive positioning disk 5 rotatory, thereby it rotates to drive sampling pipe 1, fix a position sampling pipe 1, because the quantity of sampling pipe 1 equals the quantity of shallow slot 25 or deep trouth 24, each sampling pipe 1 is corresponding with a shallow slot 25 and a deep trouth 24 respectively, stir every rotatory round of piece 26, the distance of a shallow slot 25 of positioning disk 5 rotation and a deep trouth 24, therefore, stir every rotatory round of piece 26, the distance of sampling pipe 1 of sampling pipe ring 19 rotation on the positioning disk 5.
In this embodiment, the shallow slot shifting member 28 is a sector column, the shallow slot 25 is an arc-shaped slot with a radius equal to that of the sector column, the deep slot shifting member 29 is a cylinder, and the width of the deep slot 24 is equal to the diameter of the cylinder.
In this embodiment, the upper end of the sampling tube 1 is provided with an air inlet tube 31 and an air outlet tube 32, the orifice at the top of the air inlet tube 31 is an air inlet 14 of the sampling tube 1, the orifice at the top of the air outlet tube 32 is an air outlet 13 of the sampling tube 1, the lower end of the interior of the sampling tube 1 is provided with an inclined plane 33 opposite to the air inlet tube 31, and the lower end of the air inlet tube 31 extends into the sampling tube 1 to the upper part of the inclined plane 33; bar magnet sleeve 2 is including the sleeve pipe handle 34 that from top to bottom connects gradually, the spacing ring 35, sleeve pipe pole portion 36 and sleeve pipe head portion 37, sleeve pipe handle 34 top is equipped with casing mouth 38, can insert bar magnet 3 in the bar magnet sleeve 2 through casing mouth 38, the department periphery that meets of spacing ring 35 and sleeve pipe handle 34 is equipped with sampling tube cap 39, sampling tube cap 39 lower extreme is equipped with sealing portion 40, can be with sleeve pipe head portion 37 through outlet duct 32, sleeve pipe pole portion 36 and spacing ring 35 insert in the sampling pipe 1, make sleeve pipe head portion 37 stretch into to the bottom in the sampling pipe 1, and can be connected with outlet duct 32 cooperation through spacing ring 35 and realize sealedly, when spacing ring 35 is connected with outlet duct 32 cooperation and realizes sealedly, sealing portion 40 blocks up the intake pipe 31 top simultaneously. Compared with the conventional sampling tube, the liquid at the bottom of the sampling tube directly splashes out of the air outlet pipe 32 (shown in fig. 13B) after being impacted by air entering from the air inlet pipe 31, due to the arrangement of the inclined surface 33 in the sampling tube 1, after the air enters the sampling tube 1 from the air inlet pipe 31, airflow forms vortex at the bottom of the sampling tube 1 (shown in fig. 13A), and the airflow can drive the liquid to flow, so that the liquid at the bottom of the sampling tube 1 is prevented from directly splashing out of the air outlet pipe 32 after being impacted by the air entering from the air inlet pipe 31, the loss of the sampling liquid in the sampling process is reduced, and the sampling rate is improved; the magnetic rod sleeve 2 and the sampling tube 1 can be combined, the magnetic rod sleeve 2 is inserted into the sampling tube 1, the storage space of the magnetic rod sleeve 2 and the sampling tube 1 is reduced, the storage and transportation cost is reduced, after the magnetic rod sleeve 2 is inserted into the sampling tube 1, the inner cavity of the sampling tube 1 can be sealed, impurities are prevented from entering, the sampling tube cover 39 covers the air inlet 14 and the air outlet 13 of the sampling tube 1, the sampling tube 1 is sealed and protected, and the interior of the sampling tube 1 is prevented from being polluted; sampling pipe 1 and bar magnet sleeve pipe 2 simple structure, low in production cost can regard as disposable consumptive material cover to use, avoids the cross contamination that used repeatedly arouses.
In this embodiment, a sleeve groove 41 is arranged on one side of the sleeve handle 34 close to the sealing portion 40, the magnetic rod sleeve locking device 9 includes an electromagnetic lock 42, the electromagnetic lock 42 is fixed on the first lifting structure 8 on one side of the top of the magnetic rod 3, a lock protrusion 43 is arranged on the electromagnetic lock 42, the lock protrusion 43 can be controlled to protrude out of the electromagnetic lock 42 or retract into the electromagnetic lock 42 through the electromagnetic lock 42, after the magnetic rod 3 descends and extends into the opposite magnetic rod sleeve 2, the lock protrusion 43 protrudes out of the electromagnetic lock 42 and is clamped into the sleeve groove 41, and the magnetic rod sleeve 2 is locked on the magnetic rod 3. In the power-off state, the lock projection 43 protrudes out of the electromagnetic lock 42, and in the power-on state, the lock projection 43 retracts into the electromagnetic lock 42; in a default state, the electromagnetic lock 42 is in a power-off state, before sampling, the electromagnetic lock 42 is powered on, the magnetic rod 3 descends and extends into the magnetic rod sleeve 2, after the magnetic rod 3 extends into the bottom of the magnetic rod sleeve 2, the electromagnetic lock 42 is powered off, the lock protrusion 43 protrudes out of the electromagnetic lock 42 and is clamped into the sleeve groove 41 of the magnetic rod sleeve 2, and therefore the magnetic rod sleeve 2 is fixed on the magnetic rod 3, and the magnetic rod sleeve 2 moves along with the magnetic rod 3; when the magnetic rod sleeve 2 is used up, the first lifting structure 8 stretches the magnetic rod sleeve 2 into the original sampling tube 1, the electromagnetic lock 42 is electrified, the lock protrusion 43 retracts into the electromagnetic lock 42, the first lifting structure 8 drives the magnetic rod 3 to ascend, and the magnetic rod sleeve 2 is left in the original sampling tube 1.
In this embodiment, the sealing portion 40 includes a sealing cylinder 44 and an inverted cone 45, the upper end of the sealing cylinder 44 is fixedly connected to the lower end of the sampling tube cover 39, the lower end of the sealing cylinder 44 is connected to the bottom surface of the inverted cone 45, the diameter of the sealing cylinder 44 is equal to the diameter of the bottom surface of the inverted cone 45 and equal to the inner diameter of the air inlet tube 31, when the limiting ring 35 is inserted into the air outlet tube 32 to achieve sealing, the inverted cone 45 and the sealing cylinder 44 are inserted into the air inlet tube 31 to block the top of the air inlet tube 31. The inverted cone 45 has a guiding function, so that the sealing cylinder 44 can be smoothly inserted into the air inlet pipe 31 to realize sealing.
In this embodiment, the outer diameters of the limiting ring 35, the sleeve rod portion 36 and the sleeve head portion 37 are sequentially reduced, so that the magnetic rod sleeve 2 can be conveniently inserted into the sampling tube 1, the wall thicknesses of the limiting ring 35, the sleeve rod portion 36 and the sleeve head portion 37 are equal, and the sleeve handle 34, the limiting ring 35, the sleeve rod portion 36 and the sleeve head portion 37 are integrally formed and manufactured, so that the overall structural strength is ensured, and the manufacturing is convenient; the internal diameter of intake pipe 31 is less than the internal diameter of outlet duct 32 to the velocity of flow is bigger when making air get into sampling tube 1 from intake pipe 31, with the impact dynamics of increase air to sampling liquid in sampling tube 1, improves the sampling rate.
An air microorganism sampling method adopts the automatic sampling device 100 to perform sampling, and comprises the following steps:
s1: the magnetic rod 3 moves downwards into the magnetic rod sleeve 2, the magnetic rod sleeve 2 is locked on the magnetic rod 3, and then the magnetic rod 3 moves upwards to drive the magnetic rod sleeve 2 to move out of the sampling tube 1, so that the air outlet 13 and the air inlet 14 of the sampling tube 1 are exposed;
s2: the positioning disc 5 rotates, the sampling tube 1 with the air port 13 and the air inlet 14 exposed out is rotated to be right below the sampling head 4, the sampling head 4 descends, the air exhaust channel 11 and the air inlet channel 12 are respectively communicated with the air outlet 13 and the air inlet 14 of the sampling tube 1, and the air exhaust device is started to perform air sampling;
s3: after sampling is finished, the sampling head 4 rises, and the positioning disc 5 rotates the sampling pipe 1 which finishes sampling to be right below the magnetic rod 3;
s4: the magnetic rod 3 moves downwards to drive the magnetic rod sleeve 2 to extend into the sampling tube 1, so that the magnetic beads which adsorb the air microorganism nucleic acid sample in the sampling tube 1 are adsorbed on the outer wall of the magnetic rod sleeve 2;
s5: the magnetic rod 3 moves upwards to drive the magnetic rod sleeve 2 to move out of the sampling tube 1, and magnetic beads adsorbed with air microorganism nucleic acid samples are eluted.
In the step S2, a lysis binding solution and magnetic beads are pre-stored in each sampling tube 1, and when air sampling is performed, the collected microbial sample is lysed under the action of the lysis binding solution to release nucleic acid, and the released nucleic acid can be bound to the magnetic beads, so that the effect of extracting nucleic acid while sampling is achieved.
In step S4, the cannula head 37 is a magnetic bead adsorption area, and the magnetic beads having the air microbial nucleic acid sample adsorbed thereon are adsorbed on the outer wall of the cannula head 37.
The principle and the implementation mode of the invention are explained by applying a specific example, and the description of the embodiment is only used for helping to understand the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In view of the above, the present disclosure should not be construed as limiting the invention.
Claims (10)
1. An automated sampling device, comprising: the sampling device comprises sampling tubes, magnetic rod sleeves, magnetic rods, a sampling head and a positioning disc, wherein the positioning disc is rotatably connected to a supporting shaft and driven to rotate by a rotary driving device; the magnetic rod is positioned above the sampling pipes and driven to lift by a first lifting structure, a magnetic rod sleeve locking device connected to the first lifting structure is arranged on one side of the top of the magnetic rod, the magnetic rod and the magnetic rod sleeve locking device can be driven to lift synchronously by the first lifting structure, each sampling pipe can rotate to the position right below the magnetic rod, and after the magnetic rod descends and extends into the opposite magnetic rod sleeve, the magnetic rod sleeve can be locked on the magnetic rod by the magnetic rod sleeve locking device; the sampling head is located the sampling pipe top to by the drive of second elevation structure lift, be equipped with bleed passage and inlet channel in the sampling head, bleed passage upper end is connected air exhaust device, each the sampling pipe homoenergetic rotates to under the sampling head, bleed passage with the inlet channel lower extreme respectively with just right the gas outlet and the air inlet at sampling pipe top are corresponding.
2. The automated sampling device of claim 1, wherein: the utility model discloses a sampling tube, including back shaft, support ring, sampling pipe ring cover, sampling pipe ring internal side, fixed buckle, bearing, support shaft, sampling pipe ring cover, sampling pipe ring internal side is equipped with respectively with each the fixed buckle that the spout corresponds, fixed buckle follows the spout slides in will in the fixed slot sampling pipe ring joint is fixed in on the support ring.
3. The automated sampling device of claim 1, wherein: the supporting device is characterized in that a damping shaft is fixedly arranged on the protrusion at the top end of the supporting shaft, damping protrusions are arranged on two opposite sides of the periphery of the damping shaft, and the damping protrusions are fixed on the damping shaft and supported at the upper end of the supporting ring.
4. The automated sampling device of claim 1, wherein: deep grooves and shallow grooves which are arranged in a crossed mode and are the same in number are formed in the periphery of the positioning disc, and the deep grooves and the shallow grooves are uniformly distributed in the periphery of the positioning disc; the rotary driving device comprises a stirring piece and a driving piece, wherein the stirring piece comprises a shallow groove stirring piece, a deep groove stirring piece and a connecting disc, the shallow groove stirring piece and the deep groove stirring piece are connected to two ends of the connecting disc respectively, the shallow groove stirring piece is matched with the shallow groove, the deep groove stirring piece is matched with the deep groove, when the stirring piece stirs the positioning disc, the deep groove stirring piece enters the deep groove, the shallow groove stirring piece enters the shallow groove, each stirring piece rotates for one circle, the positioning disc rotates for one shallow groove and one distance of the deep groove, the number of sampling pipes is equal to the number of the shallow groove or the deep groove, and each sampling pipe is corresponding to the shallow groove and the deep groove.
5. The automated sampling device of claim 4, wherein: the shallow groove poking piece is a fan-shaped cylinder, the shallow groove is an arc-shaped groove with the radius equal to that of the fan-shaped cylinder, the deep groove poking piece is a cylinder, and the width of the deep groove is equal to the diameter of the cylinder.
6. The automated sampling device of claim 1, wherein: the upper end of the sampling pipe is provided with an air inlet pipe and an air outlet pipe, the pipe orifice at the top of the air inlet pipe is an air inlet of the sampling pipe, the pipe orifice at the top of the air outlet pipe is an air outlet of the sampling pipe, the lower end inside the sampling pipe is provided with an inclined plane opposite to the air inlet pipe, and the lower end of the air inlet pipe extends into the sampling pipe to be above the inclined plane; the bar magnet sleeve pipe is including sleeve handle, spacing ring, sleeve pipe pole portion and the sleeve pipe head portion that from top to bottom connects gradually, the sleeve pipe handle top is equipped with the casing mouth, through the casing mouth can with the bar magnet inserts in the bar magnet sleeve, the spacing ring with the department of meeting periphery of sleeve handle is equipped with the sampling tube lid, sampling tube lid lower extreme is equipped with the sealing, through the outlet duct can with the sleeve pipe head portion sleeve pipe pole portion with the spacing ring inserts in the sampling tube, make the sleeve pipe head portion stretch into to bottom in the sampling tube, and can pass through the spacing ring with the outlet duct cooperation is connected and is realized sealedly, works as the spacing ring with the outlet duct cooperation is connected when realizing sealedly, the sealing blocks simultaneously the intake pipe top.
7. The automated sampling device of claim 6, wherein: be close to on the sleeve handle one side of sealing part is equipped with the casing groove, bar magnet sleeve pipe locking device includes the electromagnetic lock, the electromagnetic lock is fixed in bar magnet top one side on the first elevation structure, it is protruding to be equipped with the lock on the electromagnetic lock, through the electromagnetic lock can be controlled the protruding salient of lock electromagnetic lock or retraction in the electromagnetic lock, the bar magnet descends and stretches into just right the intraductal back of bar magnet sleeve, the protruding salient of lock electromagnetic lock and card go into the casing groove will the bar magnet sleeve pipe lock in on the bar magnet.
8. The automated sampling device of claim 6, wherein: the sealing part includes seal cylinder and inverted cone, seal cylinder upper end fixed connection be in sampling tube cap lower extreme, the seal cylinder lower extreme with the bottom surface of inverted cone is connected, the diameter of seal cylinder with the bottom surface diameter of inverted cone equals, and with the internal diameter of intake pipe equals, works as the spacing ring inserts when realizing sealed in the outlet duct, the inverted cone with the seal cylinder inserts the shutoff in the intake pipe top.
9. The automated sampling device of claim 6, wherein: the outer diameters of the limiting ring, the sleeve rod part and the sleeve head part are sequentially reduced, the wall thicknesses of the limiting ring, the sleeve rod part and the sleeve head part are equal, and the sleeve handle, the limiting ring, the sleeve rod part and the sleeve head part are integrally formed; the inner diameter of the air inlet pipe is smaller than that of the air outlet pipe.
10. An air microorganism sampling method is characterized in that: sampling by using the automatic sampling device of any one of claims 1 to 9, comprising the following steps:
s1: the magnetic rod moves downwards into the magnetic rod sleeve, the magnetic rod sleeve is locked on the magnetic rod, and then the magnetic rod moves upwards to drive the magnetic rod sleeve to move out of the sampling tube, so that an air outlet and an air inlet of the sampling tube are exposed;
s2: the positioning disc rotates to enable the sampling pipe with the exposed air outlet and the exposed air inlet to rotate to be right below the sampling head, the sampling head descends to enable the air exhaust channel and the air inlet channel to be communicated with the air outlet and the air inlet of the sampling pipe respectively, and the air exhaust device is started to sample air;
s3: after sampling is finished, the sampling head rises, and the positioning disc rotates the sampling pipe after sampling to be right below the magnetic rod;
s4: the magnetic rod moves downwards to drive the magnetic rod sleeve to extend into the sampling tube, so that magnetic beads of the air microorganism nucleic acid sample adsorbed in the sampling tube are adsorbed to the outer wall of the magnetic rod sleeve;
s5: the magnetic rod moves upwards to drive the magnetic rod sleeve to move out of the sampling tube, and the magnetic beads adsorbed with the air microorganism nucleic acid samples are eluted.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211086438.7A CN115161192B (en) | 2022-09-07 | 2022-09-07 | Automatic sampling device and air microorganism sampling method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211086438.7A CN115161192B (en) | 2022-09-07 | 2022-09-07 | Automatic sampling device and air microorganism sampling method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115161192A true CN115161192A (en) | 2022-10-11 |
| CN115161192B CN115161192B (en) | 2022-12-20 |
Family
ID=83481598
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211086438.7A Active CN115161192B (en) | 2022-09-07 | 2022-09-07 | Automatic sampling device and air microorganism sampling method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115161192B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116376684A (en) * | 2022-12-12 | 2023-07-04 | 烟台市农业综合执法支队 | Microorganism aerosol sampling device for livestock and veterinary |
| CN116445266A (en) * | 2023-06-14 | 2023-07-18 | 至美时代生物智能科技(北京)有限公司 | Automatic sampling and detecting device for air microorganisms |
| CN116445267A (en) * | 2023-06-14 | 2023-07-18 | 至美时代生物智能科技(北京)有限公司 | Air microorganism sampling device |
| CN116751663A (en) * | 2023-08-22 | 2023-09-15 | 内蒙古农业大学 | Microorganism collection system |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013005809A (en) * | 2012-09-05 | 2013-01-10 | Hitachi Plant Technologies Ltd | Detection object collection implement, and method for using the same |
| CN106640355A (en) * | 2016-10-19 | 2017-05-10 | 宁波大叶园林设备股份有限公司 | Improved four-stroke engine with overhead camshaft driven by toothed belt and poking plate |
| CN114606111A (en) * | 2021-12-27 | 2022-06-10 | 宁波爱基因科技有限公司 | Nucleic acid extraction tube structure, magnetic rod sleeve and nucleic acid extraction method |
| CN114774264A (en) * | 2022-06-22 | 2022-07-22 | 至美时代生物智能科技(北京)有限公司 | Automatic air microorganism detection system |
| CN114774263A (en) * | 2022-06-22 | 2022-07-22 | 至美时代生物智能科技(北京)有限公司 | Automatic detection device |
-
2022
- 2022-09-07 CN CN202211086438.7A patent/CN115161192B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013005809A (en) * | 2012-09-05 | 2013-01-10 | Hitachi Plant Technologies Ltd | Detection object collection implement, and method for using the same |
| CN106640355A (en) * | 2016-10-19 | 2017-05-10 | 宁波大叶园林设备股份有限公司 | Improved four-stroke engine with overhead camshaft driven by toothed belt and poking plate |
| CN114606111A (en) * | 2021-12-27 | 2022-06-10 | 宁波爱基因科技有限公司 | Nucleic acid extraction tube structure, magnetic rod sleeve and nucleic acid extraction method |
| CN114774264A (en) * | 2022-06-22 | 2022-07-22 | 至美时代生物智能科技(北京)有限公司 | Automatic air microorganism detection system |
| CN114774263A (en) * | 2022-06-22 | 2022-07-22 | 至美时代生物智能科技(北京)有限公司 | Automatic detection device |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116376684A (en) * | 2022-12-12 | 2023-07-04 | 烟台市农业综合执法支队 | Microorganism aerosol sampling device for livestock and veterinary |
| CN116376684B (en) * | 2022-12-12 | 2023-10-20 | 烟台市农业综合执法支队 | Microorganism aerosol sampling device for livestock and veterinary |
| CN116445266A (en) * | 2023-06-14 | 2023-07-18 | 至美时代生物智能科技(北京)有限公司 | Automatic sampling and detecting device for air microorganisms |
| CN116445267A (en) * | 2023-06-14 | 2023-07-18 | 至美时代生物智能科技(北京)有限公司 | Air microorganism sampling device |
| CN116445266B (en) * | 2023-06-14 | 2023-09-12 | 至美时代生物智能科技(北京)有限公司 | Automatic sampling and detecting device for air microorganisms |
| CN116445267B (en) * | 2023-06-14 | 2023-09-12 | 至美时代生物智能科技(北京)有限公司 | Air microorganism sampling device |
| CN116751663A (en) * | 2023-08-22 | 2023-09-15 | 内蒙古农业大学 | Microorganism collection system |
| CN116751663B (en) * | 2023-08-22 | 2024-01-16 | 鄂尔多斯市达迩科技服务有限公司 | Microorganism collection system |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115161192B (en) | 2022-12-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN115161192B (en) | Automatic sampling device and air microorganism sampling method | |
| CN115161188B (en) | Air microorganism detection system and method | |
| WO2015176548A1 (en) | Integrated processing mechanism for uniformly-mixing and filtering sample | |
| CN109370896A (en) | Cartridge is used in a kind of closed four-way multiple fluorescence PCR detection | |
| CN114045216B (en) | Air sampling bottle, air sampling system and air sampling method | |
| CN114032173B (en) | Closed air sampling bottle | |
| CN109012355B (en) | Stirring pond with adjustable be applicable to different volume sewage | |
| CN209652295U (en) | A kind of nucleic acid on-site rapidly extracting pipe | |
| CN109456880B (en) | On-site rapid nucleic acid extraction tube and use method thereof | |
| CN114774270A (en) | Automatic sampling device and sampling method | |
| CN104923395B (en) | For separating and shifting the electromagnetism electromotion integrated apparatus of magnetic-particle | |
| CN114350511A (en) | Cassette and method for rapidly detecting nucleic acid | |
| CN111378574B (en) | Nucleic acid extraction and amplification device | |
| US20230038927A1 (en) | Enricher, enrichement system, sample manufacturing system, and sample detection system | |
| CN115141739A (en) | Air microorganism sampling and detection integrated device and sampling and detection method | |
| CN206986177U (en) | A kind of integrated purification of nucleic acid extractor | |
| CN210113275U (en) | Anti-pollution nucleic acid extraction system | |
| CN115161173B (en) | Magnetic rod sleeve, sampling pipe structure and air microorganism sampling method | |
| CN217149199U (en) | Automatic sampling device | |
| CN105441318A (en) | Nucleic acid product extraction device configured with barrier | |
| CN210945545U (en) | High-flux micro-fluidic rapid nucleic acid extraction and detection device | |
| CN206232701U (en) | Magnetic frame with liquid suction function | |
| CN207435462U (en) | A kind of hermetic multiple fluorescent PCR detection cartridge | |
| CN217677535U (en) | Microorganism acquisition and detection device | |
| CN220364542U (en) | Nucleic acid extraction device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |