CN114774263A - Automatic detection device - Google Patents
Automatic detection device Download PDFInfo
- Publication number
- CN114774263A CN114774263A CN202210707654.2A CN202210707654A CN114774263A CN 114774263 A CN114774263 A CN 114774263A CN 202210707654 A CN202210707654 A CN 202210707654A CN 114774263 A CN114774263 A CN 114774263A
- Authority
- CN
- China
- Prior art keywords
- chip
- magnetic needle
- needle sleeve
- magnetic
- station
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Computer Hardware Design (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides an automatic detection device, which relates to the technical field of microorganism detection equipment and comprises the following components: the device comprises a magnetic bead adsorption transfer mechanism, a chip storage mechanism, a centrifugal heating mechanism, a sampling bottle and a control unit; the sampling bottle is used for containing magnetic beads adsorbed with nucleic acid, a plurality of chips used for detecting microorganisms are stored in the chip storage mechanism, the magnetic bead adsorption and transfer mechanism is used for adsorbing and transferring the magnetic beads in the sampling bottle to a sample adding port of the chip in the chip storage mechanism, reaction liquid is added in the chip in advance, the magnetic bead adsorption and transfer mechanism can elute the nucleic acid from the magnetic beads in the reaction liquid at the sample adding port of the chip, the chip transfer mechanism is used for transferring the chip containing the reaction liquid and the nucleic acid to the centrifugal heating mechanism, and the centrifugal heating mechanism is used for sequentially centrifuging and heating the chip; the scheme provided by the invention reduces the labor cost and reduces the risk of pollution detection reaction.
Description
Technical Field
The invention relates to the technical field of microorganism detection equipment, in particular to an automatic detection device.
Background
The air microorganism detection is significant for infectious disease prevention and control and environmental hygiene supervision and protection, the air microorganism detection needs to collect an air microorganism sample, a lysis solution is used as a sampling solution, the nucleic acid adsorption magnetic beads are placed in the lysis solution for air microorganism sample collection, and the collected air microorganism sample can be lysed in the sampling process and the nucleic acid released by microorganism sample lysis is adsorbed on the nucleic acid adsorption magnetic beads. The micro-fluidic chip is widely applied in the field of microbial detection, however, the existing chip needs to be subjected to multiple steps when used for detecting microbial nucleic acid, and the steps are all carried out manually, so that the labor cost is increased, and the risk of pollution detection reaction is increased.
Disclosure of Invention
The invention aims to provide an automatic detection device, which solves the problems in the prior art, reduces the labor cost and reduces the risk of nucleic acid pollution.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides an automatic detection device, comprising: the device comprises a magnetic bead adsorption transfer mechanism, a chip storage mechanism, a centrifugal heating mechanism, a sampling bottle and a control unit; the control unit controls the magnetic bead adsorption and transfer mechanism, the centrifugal heating mechanism and the chip transfer mechanism to work;
the sampling bottle is used for containing magnetic beads with adsorbed nucleic acid, a plurality of chips used for detecting microorganisms are stored in the chip storage mechanism, the magnetic beads are adsorbed and transferred to the magnetic beads in the sampling bottle, the sample port of the chip is stored in the chip storage mechanism, a reaction liquid is added in the chip in advance, the magnetic beads are adsorbed and transferred to the reaction liquid at the sample port of the chip, the magnetic beads are eluted by the reaction liquid, the chip transfer mechanism is used for transferring the chip containing the reaction liquid and the nucleic acid to the centrifugal heating mechanism, and the centrifugal heating mechanism is used for sequentially centrifuging and heating the chip.
Preferably, the chip storage mechanism comprises a storage cavity and two first limiting plates, the top of the storage cavity is open, an elastic support member is arranged in the storage cavity, the first limiting plate is fixedly arranged above the top of the storage cavity, the distance between the first limiting plate and the top of the storage cavity is not less than the thickness value of one chip, a plurality of chips are stacked between the elastic support member and the first limiting plate, the elastic support member is in a compression state, the sample injection port of each chip faces upwards, the inner side wall of the storage cavity is used for limiting the horizontal movement freedom degree of the chips in the storage cavity, the two first limiting plates are respectively pressed on two opposite sides of the chips, an interval extending along a first linear direction is arranged between the two first limiting plates, one side of an area between the top of the storage cavity and the first limiting plate is a chip outlet, and a push strip vertical to the first straight line is fixedly arranged on the upper surface of the chip.
Preferably, be provided with the detection station in the centrifugal heating mechanism, the detection station is located the mechanism is stored to the chip is equipped with one side of chip export, chip transfer mechanism includes push pedal and push pedal drive arrangement, push pedal drive arrangement is used for the drive the push pedal is followed first straight line removes, the default position of push pedal is located the storage chamber is kept away from one side of detection station, the push pedal is followed first straight line can promote the top during the direction of detection station removes push away the strip on the chip and will the chip pushes away to on the detection station.
Preferably, chip transfer mechanism still includes the fixed plate, the vertical setting of fixed plate just the fixed plate with push pedal drive arrangement's drive end fixed connection, the top of push pedal rotate connect in the bottom of fixed plate, the push pedal orientation the one side of detecting the station is the front, and the another side is the back, the back side of push pedal is provided with prevents the flitch, just prevent flitch fixed connection in on the fixed plate, prevent that the flitch is used for the restriction the push pedal rotates to the back side, the push pedal can rotate to the front side when receiving the effort to the front side.
Preferably, the magnetic bead adsorption and transfer mechanism comprises a magnetic needle driving device, a magnetic needle sleeve storage device and a magnetic needle sleeve waste barrel; the sampling bottle top is uncovered, magnetism needle sleeve pipe strorage device is used for depositing the magnetism needle sleeve pipe, magnetism needle drive arrangement can drive the magnetism needle and get into magnetism needle sleeve pipe strorage device cover is established the magnetism needle sleeve pipe, and the drive the magnetism needle and the magnetism needle sleeve pipe stretches into adsorb in the sampling bottle the magnetic bead, then the drive the magnetism needle sleeve pipe removes to the sample addition mouth department of chip is right nucleic acid on the magnetic bead elutes, drives at last the magnetism needle sleeve pipe removes to in the abandonment bucket of magnetism needle sleeve pipe will the magnetism needle sleeve pipe is abandoned.
Preferably, the magnetic needle can be inserted into the magnetic needle sleeve, one end of the magnetic needle extending into the magnetic needle sleeve is a bottom end, the other end of the magnetic needle is a top end, a first protrusion extending along the circumferential direction is arranged on the outer wall of the magnetic needle close to the top end, a groove extending along the circumferential direction is arranged on the inner wall of the magnetic needle sleeve close to the top end, the magnetic needle sleeve is made of an elastic material, the first protrusion can be clamped in the groove when the magnetic needle is inserted into the magnetic needle sleeve, and a limit ring extending away from the magnetic needle sleeve is fixedly sleeved on the outer wall of the magnetic needle sleeve;
magnetic needle sleeve pipe strorage device possesses a plurality of magnetic needle sleeve pipes and deposits the station, it is open-topped cavity to deposit the station, one vertically placed in depositing the station the magnetic needle sleeve pipe, just the top opening of magnetic needle sleeve pipe is up, magnetic needle drive arrangement can drive the bottom of magnetic needle vertically stretch into in the magnetic needle sleeve pipe until first protruding joint in the recess.
Preferably, the magnetic bead adsorption transfer mechanism further comprises a magnetic needle sleeve storage device driving device and a magnetic needle sleeve waste barrel driving device; the magnetic needle sleeve abandoning barrel is internally provided with at least one abandoning station which is a cavity with an opening at the top, the magnetic needle driving device can drive the magnetic needle sleeved with the magnetic needle sleeve to move to the position right above the abandoning station and drive the magnetic needle to drive a limiting ring on the magnetic needle sleeve to enter the abandoning station, and a second limiting plate is fixedly arranged at a part of an opening of the abandoning station;
when the spacing ring on the magnetic needle sleeve is located abandon the station, magnetic needle sleeve pipe abandonment bucket drive arrangement can drive magnetic needle sleeve pipe abandonment bucket removes extremely the second limiting plate is in directly over the spacing ring, at this moment, magnetic needle drive arrangement drive the magnetic needle court when abandoning the station and moving outward, the second limiting plate prevents the spacing ring makes the magnetic needle sleeve pipe is followed drop on the magnetic needle.
Preferably, the plurality of storage stations are uniformly distributed around a first circumference, the magnetic needle cannula storage device driving device can drive the magnetic needle cannula storage device to rotate around a first vertical axis, and the first vertical axis passes through the circle center of the first circumference;
a plurality of discarding stations are arranged in the magnetic needle sleeve waste barrel and are arranged along a second circumference, the discarding stations extend along the second circumference, the magnetic needle sleeve waste barrel driving device can drive the magnetic needle sleeve waste barrel to rotate around a second vertical shaft, and the second vertical shaft passes through the circle center of the second circumference;
sampling bottle with the chip stores the mechanism top the orthographic projection of sample loading port of chip on a horizontal plane is located the third circumference, arbitrary one abandon the station and arbitrary one deposit the station and be in orthographic projection on the horizontal plane also can be located on the third circumference, magnetic needle drive arrangement can pass through the rotating electrical machines drive the magnetic needle around the center pin of third circumference rotates.
Preferably, the disposal station comprises a limit cavity and an insertion cavity, the insertion cavity and the limit cavity both extend along the second circumference, the inserting cavity and the limiting cavity are sequentially distributed along the second circumference, the inserting cavity is communicated with the limiting cavity, two opposite side walls of the limiting cavity are respectively provided with a limiting groove, the two limiting grooves respectively extend along a fourth circumference and a fifth circumference, the fourth circumference and the fifth circumference are concentric with the second circumference, the stop collar is capable of extending into the insertion cavity, when on the magnetic needle sleeve pipe the spacing ring with the spacing groove is right-time, rotate around the vertical axle of second magnetic needle sleeve pipe abandonment bucket can make the magnetic needle sleeve pipe gets into spacing chamber and the spacing ring gets into the spacing inslot, directly over the spacing groove the chamber wall structure in spacing chamber is promptly the second limiting plate.
Preferably, the centrifugal heating mechanism comprises a centrifugal disc, a centrifugal disc driving device, a heating plate, a first conductive piece, a second conductive piece driving device and a centrifugal disc position adjusting device, the control unit controls the centrifugal disc driving device, the second conductive piece driving device and the centrifugal disc position adjusting device to work, a detection station for testing a chip is arranged on the centrifugal disc, the heating plate is embedded and fixed in the centrifugal disc, the first conductive piece is arranged on the side edge of the centrifugal disc and is electrically connected with the heating plate, the centrifugal disc driving device is used for driving the centrifugal disc to carry out centrifugal treatment on the chip, and the heating plate is used for heating the chip in the detection station; the default position of the second conductive piece is far away from the first conductive piece, the second conductive piece driving device can drive the second conductive piece to a first station, the centrifugal disc position adjusting device can adjust the position of the centrifugal disc to enable the first conductive piece to be located at a second station, the second conductive piece located at the first station is in contact with the first conductive piece located at the second station, and the second conductive piece is electrically connected with a power supply.
Preferably, the system also comprises a detector and a detector driving device, wherein the detector is used for detecting the reaction result of the chip and transmitting the detection result to the man-machine interaction terminal, and the detector driving device can drive the detector to rotate to the position right above the detection station.
Compared with the prior art, the invention achieves the following technical effects:
the automatic detection device provided by the invention controls the magnetic bead adsorption and transfer mechanism, the centrifugal heating mechanism and the chip transfer mechanism to work through the control unit, and does not need manual magnetic bead transfer, elution and chip transfer, so that the labor cost is reduced, and the risk of pollution detection reaction is reduced.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an automatic inspection apparatus provided in the present invention (a chip transfer mechanism is not shown);
FIG. 2 is a schematic structural view of a magnetic needle;
FIG. 3 is a schematic structural view of a magnetic needle cannula;
FIG. 4 is a schematic view of a configuration in which two magnetic needle cannulae are placed in a magnetic needle cannula storage device;
FIGS. 5 to 7 are schematic structural views of the magnetic needle cannula discarded in the magnetic needle cannula discarding barrel in three states;
FIG. 8 is a schematic structural view of a centrifugal heating mechanism;
FIG. 9 is a top view of FIG. 8;
FIG. 10 is a schematic diagram of a chip;
FIG. 11 is an external view of the chip storage mechanism without a liquid filling structure;
FIG. 12 is an external view of the chip storage mechanism with a chip disposed therein and the liquid adding structure in a liquid adding state;
FIG. 13 is a schematic view of a plurality of chips and an elastic support;
FIG. 14 is a schematic structural view of a push plate;
FIG. 15 is another side view of the automated inspection device provided in the present invention (showing the chip transfer mechanism);
in the figure: 1-sampling bottle, 2-centrifugal heating mechanism, 21-detection station, 22-centrifugal disc, 221-arc-shaped protrusion, 23-adjusting gear, 24-second conductive piece, 25-linear slide rail, 26-first conductive piece, 27-first photoelectric sensor, 28-centrifugal disc driving device, 29-second photoelectric sensor, 201-garbage can, 202-shading sheet, 3-magnetic needle sleeve abandon barrel, 31-abandon station, 311-insertion cavity, 312-limit cavity, 313-limit groove, 314-second limit plate, 4-magnetic needle sleeve storing device, 41-storing station, 5-magnetic needle, 51-first protrusion, 6-magnetic needle driving device, 7-chip storing mechanism, 71-storing cavity, 72-a first limit plate, 73-a steering engine plate, 74-a liquid feeding pipe, 75-a reaction liquid storage bottle, 76-a peristaltic pump, 77-an elastic support member, 771-a support plate, 772-a spring, 8-a chip transfer mechanism, 81-a push plate, 82-a fixing plate, 83-an anti-rotation plate, 9-a magnetic needle sleeve, 91-a limiting ring, 92-a groove, 10-a chip, 101-a reaction chamber, 102-a sample adding port, 103-a pushing strip, 104-a limiting strip, 11-a driving device body, 12-a sliding block and 13-a rack.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention aims to provide an automatic detection device, which solves the problems in the prior art, reduces the labor cost and reduces the risk of pollution detection reaction.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
The present invention provides an automatic detection device, as shown in fig. 1 to 15, comprising: the device comprises a magnetic bead adsorption transfer mechanism, a chip transfer mechanism 8, a chip storage mechanism 7, a centrifugal heating mechanism 2, a sampling bottle 1 and a control unit; the control unit controls the magnetic bead adsorption transfer mechanism, the centrifugal heating mechanism 2 and the chip transfer mechanism 8 to work;
The automatic detection device provided by the invention controls the magnetic bead adsorption and transfer mechanism, the centrifugal heating mechanism 2 and the chip transfer mechanism 8 to work through the control unit, and does not need to manually transfer and elute magnetic beads and transfer the chip 10, so that the labor cost is reduced, and the risk of pollution detection reaction is reduced.
When the device is put into operation, the control unit controls the structure to sequentially perform the following operations: controlling the magnetic bead adsorption and transfer mechanism to adsorb the magnetic beads, transferring the magnetic beads to the sample addition port 102 of the chip 10, eluting nucleic acid on the magnetic beads at the sample addition port 102, transferring the chip 10 to the centrifugal heating mechanism 2 by the chip transfer mechanism 8, and sequentially centrifuging and heating the chip by the centrifugal heating mechanism 2.
Further, the chip storage mechanism 7 includes a storage cavity 71 and two first limiting plates 72, the top of the storage cavity 71 is open, an elastic support 77 is disposed in the storage cavity 71, the first limiting plate 72 is fixedly disposed above the top of the storage cavity 71, a distance between the first limiting plate 72 and the top of the storage cavity 71 is not less than a thickness value of one chip 10, a plurality of chips 10 are stacked between the elastic support 77 and the first limiting plate 72, the elastic support 77 is in a compressed state, the sample addition port 102 of each chip 10 faces upward, the inner side wall of the storage cavity 71 is used for limiting the horizontal movement freedom of the chips 10 in the storage cavity 71, the two first limiting plates 72 are respectively pressed on two opposite sides of the chip 10, an interval extending along a first linear direction is formed between the two first limiting plates 72, one side of an area between the top of the storage cavity 71 and the first limiting plate 72 is a chip outlet, the upper surface of the chip 10 is fixedly provided with a push strip 103 perpendicular to the first straight line, the topmost chip 10 in the chip storage mechanism 7 can be pushed to the detection station 21 along the first straight line by the push plate 81, and after the topmost chip 10 is moved out, the plurality of stored chips 10 are lifted integrally under the elastic action of the elastic support member 77 and supplemented below the first limit plate 72, so that the next action can be performed.
Further, the elastic supporting member 77 includes a spring 772 and a supporting plate 771, the supporting plate 771 and the bottom surface of the storage cavity 71 are both horizontally disposed, the spring 772 is disposed between the supporting plate 771 and the bottom surface of the storage cavity 71, and a plurality of chips 10 are stacked between the supporting plate 771 and the first limiting plate 72.
Further, in order to add the reaction solution into the chip 10, the chip storage mechanism 7 further includes a liquid adding structure, and the liquid adding structure is used for adding the reaction solution into the sample addition port 102 of the topmost chip 10.
Further, the liquid adding structure comprises a reaction liquid storage bottle 75, a peristaltic pump 76, a base, a liquid adding pipe 74, a steering engine and a steering engine plate 73, wherein the base is fixedly arranged on one side of the storage cavity 71, the steering engine is fixed on the base, the steering engine plate 73 is fixed on the steering engine, and the steering engine can drive the steering engine plate 73 to rotate along a horizontal shaft; one end of the liquid feeding pipe 74 is connected with a reaction liquid storage bottle 75, the other end extends to the rudder plate 73, the tail end of the reaction solution storage bottle 75 is inserted and fixed on the rudder plate 73, the peristaltic pump 76 is used for pumping the reaction solution in the reaction solution storage bottle 75 to the sample addition port 102, the rudder plate 73 is in a vertical state as a default, when the steering engine plate 73 rotates 90 degrees to the position above the chip 10, the outlet of the liquid feeding pipe 74 is positioned right above the sample feeding port 102 of the topmost chip 10, when liquid needs to be fed, the steering engine controls the steering engine plate 73 to rotate 90 degrees to the position above the chip 10, at the moment, the outlet of the liquid feeding pipe 74 is positioned right above the sample feeding port 102 of the topmost chip 10, the peristaltic pump 76 is started, the peristaltic pump 76 pumps the liquid in the reaction liquid storage bottle 75 into the chip 10, after the liquid adding process is completed, the peristaltic pump 76 stops working, and the steering engine controls the rudder plate 73 to return to a default position (namely, a vertical state) so as to prevent the rudder plate 73 from blocking the push plate 81 to push the chip 10.
Further, the centrifugal heating mechanism 2 is provided with a detection station 21, the detection station 21 is located on one side of the chip storage mechanism 7 provided with a chip outlet, the chip transfer mechanism 8 comprises a push plate 81 and a push plate driving device, the push plate driving device is used for driving the push plate 81 to move along a first straight line, a default position of the push plate 81 is located on one side of the storage cavity 71 away from the detection station 21, the push plate 81 can push the push strip 103 on the topmost chip 10 and push the chip 10 to the detection station 21 when moving along the first straight line towards the detection station 21, the chip transfer mechanism 8 is used for transferring the chip 10 in the chip storage mechanism 7 to the detection station 21, and the push plate driving device drives the push plate 81 to push the chip 10 towards the detection station 21, and the process does not need manual intervention, so that the chip transfer mechanism 8 in the scheme provided by the invention achieves the purpose of automatically transferring the chip 10, so as to realize the automatic detection of the microorganisms and reduce the risk of pollution detection reaction.
Further, the chip transfer mechanism 8 further includes a fixing plate 82, the fixing plate 82 is vertically disposed, the fixing plate 82 is fixedly connected to the driving end of the push plate driving device, the top end of the push plate 81 is rotatably connected to the bottom end of the fixing plate 82, and the push plate 81 is rotatably connected to the fixing plate 82 through a connecting shaft, one surface of the push plate 81 facing the detection station 21 is a front surface, and the other surface of the push plate 81 is a back surface, a rotation preventing plate 83 is disposed on the back surface of the push plate 81, and the rotation preventing plate 83 is fixedly connected to the fixing plate 82, the rotation preventing plate 83 is used for preventing the push plate 81 from rotating to the back surface side, when the push plate 81 receives an acting force to the front surface side, so that when the push plate 81 pushes the chip 10 to move forward along the front surface side direction, the push plate 81 cannot rotate in an opposite direction under the restriction of the rotation preventing plate 83; when the pushing plate 81 finishes the operation of pushing the chip 10 and returns to the default position along the original route, when the pushing plate 81 encounters an obstacle (e.g., the chip 10 in the chip storage mechanism 7), it can be pushed by the obstacle and rotate along the direction of the front side with the connecting shaft as the axis, smoothly pass through the obstacle and return to the default position; wherein, the bottom edge of the back side of the push plate 81 is chamfered, so that the push plate 81 can pass through the obstacle more easily.
Further, the magnetic bead adsorption transfer mechanism comprises a magnetic needle driving device 6, a magnetic needle sleeve storage device 4 and a magnetic needle sleeve waste barrel 3; 1 top of sampling bottle is uncovered, magnetic needle sleeve pipe strorage device 4 is used for depositing magnetic needle sleeve pipe 9, magnetic needle drive arrangement 6 can drive magnetic needle 5 and get into 4 covers of magnetic needle sleeve pipe strorage device and establish magnetic needle sleeve pipe 9, and drive magnetic needle 5 and magnetic needle sleeve pipe 9 and stretch into absorption magnetic bead in the sampling bottle 1, then drive magnetic needle sleeve pipe 9 and remove the nucleic acid elution to the magnetic bead of sample addition mouth 102 department of chip 10, drive magnetic needle sleeve pipe 9 at last and remove and abandon magnetic needle sleeve pipe 9 in magnetic needle sleeve pipe abandonment bucket 3.
Further, the magnetic needle 5 can be inserted into the magnetic needle sleeve 9, one end of the magnetic needle 5, which extends into the magnetic needle sleeve 9, is a bottom end, the other end is a top end, a first protrusion 51, preferably two protrusions, which extends in the circumferential direction, is arranged on the outer wall of the magnetic needle 5, which is close to the top end, the inner wall of the magnetic needle sleeve 9, which is close to the top end, is provided with a groove 92, which extends in the circumferential direction, and the two protrusions are also arranged, the magnetic needle sleeve 9 is made of an elastic material, the magnetic needle sleeve 9 can slightly elastically deform when stressed, when the magnetic needle 5 is inserted into the magnetic needle sleeve 9, the first protrusion 51 can be clamped into the groove 92, and the outer wall of the magnetic needle sleeve 9 is further fixedly sleeved with a limit ring 91, which extends in a direction away from the magnetic needle sleeve 9; the limit ring 91 is arranged at the top end of the magnetic needle sleeve 9, the limit ring 91 and the magnetic needle sleeve 9 are integrally formed, and the magnetic beads can be adsorbed after the magnetic needle sleeve 9 is sleeved on the magnetic needle 5, so that the structure meets the aim of inserting and fixing the magnetic needle sleeve 9 on the magnetic needle 5;
magnetic needle sleeve pipe strorage device 4 possesses a plurality of magnetic needle sleeve pipes and deposits station 41, it is open-topped cavity to deposit station 41, one deposits in station 41 and vertically places a magnetic needle sleeve pipe 9, and magnetic needle sleeve pipe 9's top end opening up, magnetic needle drive arrangement 6 is arranged in the bottom of drive magnetic needle 5 is vertical to stretch into in magnetic needle sleeve pipe 9 until first protruding 51 joint in recess 92, above-mentioned process need not manual intervention, so that realize carrying out automated inspection to the microorganism, and reduced the risk of pollution detection reaction.
Further, the magnetic bead adsorption and transfer mechanism also comprises a magnetic needle sleeve storage device driving device and a magnetic needle sleeve waste barrel driving device; at least one discarding station 31 is arranged in the magnetic needle sleeve discarding barrel 3, the discarding station 31 is a cavity with an opening at the top, the magnetic needle driving device 6 can drive the magnetic needle 5 sleeved with the magnetic needle sleeve 9 to move right above the discarding station 31 and drive the magnetic needle 5 to drive the limiting ring 91 on the magnetic needle sleeve 9 to enter the discarding station 31, and a second limiting plate 314 is fixedly arranged at a part of an opening of the discarding station 31;
when spacing ring 91 on the magnetic needle sleeve 9 is located abandoning station 31, magnetic needle sleeve abandonment bucket drive arrangement can drive magnetic needle sleeve abandonment bucket 3 and remove to second limiting plate 314 and be in directly over the spacing ring 91, and at this moment, when magnetic needle drive arrangement 6 drive magnetic needle 5 removed outside abandoning station 31, second limiting plate 314 prevented spacing ring 91 and made magnetic needle sleeve 9 drop from magnetic needle 5, and then realized abandoning magnetic needle sleeve 9's purpose automatically.
Further, the plurality of storage stations 41 are uniformly distributed around the first circumference, the magnetic needle cannula storage device driving device can drive the magnetic needle cannula storage device 4 to rotate around a first vertical axis, and the first vertical axis passes through the circle center of the first circumference;
a plurality of discarding stations 31 are arranged in the magnetic needle sleeve waste barrel 3, the plurality of discarding stations 31 are arranged along a second circumference, the discarding stations 31 extend along the second circumference, the magnetic needle sleeve waste barrel driving device can drive the magnetic needle sleeve waste barrel 3 to rotate around a second vertical shaft, and the second vertical shaft passes through the circle center of the second circumference;
sampling bottle 1 and chip store mechanism 7 overhead chip 10's the orthographic projection of sample loading port 102 on a horizontal plane and be located the third circumference, arbitrary abandoning station 31 and arbitrary one deposit station 41 orthographic projection on the horizontal plane also can be located the third circumference, magnetic needle drive arrangement 6 can drive magnetic needle 5 through the rotating electrical machines and rotate around the center pin of third circumference, the radius of rotation of magnetic needle equals the radius of third circumference, the structure of magnetic needle drive arrangement 6 has been simplified to the above-mentioned structure, magnetic needle drive arrangement 6 only need possess the rotatory and function that goes up and down of drive magnetic needle, with simple structure alright realize the cover of magnetic needle sleeve pipe 9 and establish, the absorption of magnetic bead, the elution of nucleic acid and the process of abandoning of magnetic needle sleeve pipe 9.
Further, abandon station 31 and include spacing chamber 312 and insert chamber 311, insert chamber 311 and spacing chamber 312 and all extend along the second circumference, and insert chamber 311 and spacing chamber 312 and lay along the second circumference in proper order, insert chamber 311 and spacing chamber 312 and link up, a spacing groove 313 has all been seted up on two relative lateral walls in spacing chamber 312, two spacing grooves 313 extend along fourth circumference and fifth circumference respectively, fourth circumference and fifth circumference are concentric with the second circumference, spacing ring 91 can stretch into and insert in the chamber 311, spacing ring 91 on magnetic needle sleeve 9 is right-timing with spacing groove 313, it can make magnetic needle sleeve 9 get into spacing chamber 312 and spacing ring 91 get into in the spacing groove 313 to rotate magnetic needle sleeve abandonment bucket 3 around the vertical axle of second, the chamber wall structure of spacing chamber 312 directly over spacing groove 313 is second limiting plate 314.
Further, the centrifugal heating mechanism 2 includes a centrifugal tray 22, a centrifugal tray driving device 28, a heating plate, a first conductive member 26, a second conductive member 24, a second conductive member driving device, and a centrifugal tray position adjusting device, the control unit controls the centrifugal tray driving device 28, the second conductive member driving device, and the centrifugal tray position adjusting device to operate, the centrifugal tray 22 is provided with a detection station 21 for testing the chip 10, the heating plate is embedded and fixed in the centrifugal tray 22, the first conductive member 26 is arranged on a side edge of the centrifugal tray 22, the first conductive member 26 is electrically connected with the heating plate, the centrifugal tray driving device 28 is used for driving the centrifugal tray 22 to perform centrifugal processing on the chip 10, and the heating plate is used for heating the chip 10 in the detection station 21; the default position of the second conductive member 24 is far away from the first conductive member 26, the driving device of the second conductive member 24 can drive the second conductive member 24 to the first working position, the position adjusting device of the centrifugal disc 22 can adjust the position of the centrifugal disc 22 so that the first conductive member 26 is located at the second working position, the second conductive member 24 at the first working position is in contact with the first conductive member 26 at the second working position, and the second conductive member 24 is electrically connected with a power supply.
The centrifugal heating mechanism 2 can carry out centrifugal treatment and heating treatment on the chip 10 in sequence, so that the intermediate transfer link is omitted, and the processes are automatically carried out under the control of the control unit, therefore, the risk of pollution detection reaction is reduced, and the labor cost is reduced.
Further, the centrifugal disc 22 position adjusting device includes an adjusting gear 23 and an adjusting gear driving motor, the centrifugal disc 22 is circular, a fixed gear is fixedly arranged below the centrifugal disc 22, the fixed gear and the centrifugal disc 22 are coaxially arranged, the fixed gear and the adjusting gear 23 can be meshed with each other, the adjusting gear driving motor is fixedly arranged on the second conductive member driving device, the adjusting gear 23 is fixedly arranged on a driving shaft of the adjusting gear driving motor, and when the second conductive member driving device drives the second conductive member 24 to the first station, the adjusting gear 23 can be just meshed with the fixed gear; the adjusting gear driving motor can drive the adjusting gear 23 to rotate and drive the centrifugal disc 22 to rotate until the first conductive piece 26 rotates to the second station and contacts with the second conductive piece 24 at the first station. The first conductive member 26 and the second conductive member 24 are contacted to achieve the effect of mutual conduction, and the centrifugal plate 22 is driven to rotate by using the gear transmission principle with better reliability.
Further, the outer fringe of centrifugal tray 22 is fixed and is provided with light shield 202, the outside of centrifugal tray 22 is fixed and is provided with first photoelectric sensor 27, first photoelectric sensor 27's response end is provided with the cell body towards centrifugal tray 22, light shield 202 can move to in the cell body and be sensed in the cell body, light shield 202 moves to first photoelectric sensor 27 department and is responded to by first photoelectric sensor 27 then, first electrically conductive piece 26 just in time is located the second station, first photoelectric sensor 27 and the control unit communication connection, when first photoelectric sensor 27 senses light shield 202, the control unit controls adjusting gear 23 driving motor stop work promptly, the effect that automatic control adjusting gear 23 driving motor stopped starting and stopping has been realized to above-mentioned design.
Further, the second conductive member driving device includes a sliding block 12 and a driving device body 11, the sliding block 12 is connected to the frame 13 in a sliding manner along a direction away from and close to the centrifugal disk 22, a sliding rail matched with the sliding block 12 is a linear sliding rail 25, and the second conductive member 24 and the adjustment gear driving motor are both fixedly arranged on the sliding block 12.
Further, a garbage can 201 is further arranged on one side of the detection station 21, the garbage can 201 is used for containing the waste chips 10, the detection station 21 is of a rectangular structure, three surrounding edges are fixedly arranged on the peripheral side of the detection station 21, and a chip inlet and a chip outlet are arranged on the side without the surrounding edges; the top of the two oppositely arranged surrounding edges is also provided with a top plate which is used for limiting the freedom degree of the upward movement of the chip 10, the arrangement can improve the rotation stability of the chip 10 in the centrifugal process and prevent the chip 10 from being thrown out in the centrifugal process; the surrounding edge at one side opposite to the inlet and the outlet of the chip is not provided with a top plate, and the height of the side wall plate is lower than that of the chip 10, so that the pushing plate can smoothly push the chip 10 to move through the surrounding plate;
the centrifugal heating mechanism 2 further comprises a second photoelectric sensor 29 fixed on the peripheral side of the centrifugal disc 22, and when the second photoelectric sensor 29 senses the light shielding sheet 202, the inlet and outlet of the chip are opposite to the garbage bin 201; the push plate driving device can drive the push plate 81 to push the chip 10 out of the chip inlet and outlet and drop the chip into the garbage can 201, the second photoelectric sensor 29 is in communication connection with the control unit, after the test is finished, the adjusting gear driving motor drives the adjusting gear 23 to drive the centrifugal disc 22 to rotate, and when the second photoelectric sensor 29 senses the shading sheet 202, the control unit controls the adjusting gear driving motor to stop driving; at this time, the chip inlet and outlet is right opposite to the trash can 201, and the control unit controls the push plate driving device to drive the push plate 81 to push the chip 10 out of the chip inlet and outlet and drop the chip into the trash can 201, so as to further improve the automation, wherein the distance between the first photoelectric sensor 27 and the second photoelectric sensor 29 from the center of the centrifugal disk is greater than the distance between the first conductive member 26 and the center of the centrifugal disk, so that the first conductive member 26 is prevented from moving into the groove of the first photoelectric sensor 27 or the second photoelectric sensor 29 and being sensed during the rotation of the centrifugal disk.
Through the positioning of the first photoelectric sensor 27, the inlet and the outlet of the chip can also be opposite to the chip storage mechanism 7, so that the chip 10 can be conveniently pushed into the detection station 21 from the chip storage mechanism 7; the second conductive member 24 is electrically connected to a power source, but the power source is in a default state of off; when the chip 10 is pushed from the chip storage mechanism 7 to the detection station 21, although the first conductive member 26 is located at the second station, the second conductive member 24 is located at the first station, and the first conductive member 26 is in contact with the second conductive member 24, the power supply is in a closed state, and the heating plate does not start heating; when the chip 10 is centrifuged at the inspection station 21 and the first conductive member 26 is driven again to contact the second conductive member 24, the control unit controls the power connected to the second conductive member 24 to be turned on, so that the heating plate is heated.
The structure of the chip 10 is shown in fig. 10, each reaction chamber 101 is located on the periphery of the sample addition port 102 and is communicated with the sample addition port 102 through a flow channel, under the action of centrifugal force, the reaction liquid at the sample addition port 102 can flow into each reaction chamber 101 on the periphery, the upper surface of the chip 10 is provided with a push strip 103 and two limit strips 104, the upper surfaces of the push strip 103 and the two limit strips 104 are located on the same horizontal plane, the push strip 103 and the two limit strips 104 respectively correspond to three surrounding edges on the periphery of the detection station 21, when the chip is located at the detection station, the push strip 103 is in contact with the surrounding edge without a top plate at the top, the two limit strips 104 are in contact with the two top plates respectively, and the chip edges outside the two limit strips 104 are located below the two top plates respectively, the above arrangement makes the center of gravity of the chip 10 deviate from the center position of the chip 10, the side with the push strip 103 is deviated to one side, through the eccentric arrangement of the center of the chip 10 and the surrounding edges and the top plates, the chips 10 can be prevented from being thrown out in the centrifugal process, the stability of the detection process is improved, and meanwhile, the two limiting strips 104 can enable a plurality of chips 10 to be horizontally stacked in the chip storage mechanism 7;
with respect to the pusher 81, the height of the lowermost end of the pusher 81 is lower than the height of the top of the pushing bar 103 of the uppermost chip 10 in the chip storage mechanism 7 and higher than the height of the surrounding edge, so as not to affect the pusher 81 to push the chip 10 on the inspection station 21 into the trash can 201.
Further, the first conductive element 26 is a conductive strip, the outer edge of the conductive strip is arc-shaped, and the second conductive element 24 is a conductive rod.
Furthermore, an arc-shaped protrusion 221 is fixedly disposed on a side of the centrifugal plate 22, the conductive sheet is disposed on an outermost edge of the arc-shaped protrusion 221, and the conductive sheet can elastically stretch in a horizontal direction and in a direction away from and close to the arc-shaped protrusion 221, so as to enhance flexibility of contact and separation between the first conductive member 26 and the second conductive member 24.
The centrifugal heating method of the centrifugal heating mechanism 2 includes:
the method comprises the following steps: the control unit controls the centrifugal disc driving device 28 to drive the centrifugal disc 22 to carry out centrifugal treatment on the chip 10, so that the mixed solution of the reaction solution and the nucleic acid at the sample adding port 102 flows into the reaction chamber 101, and then the centrifugation is stopped;
step two: the control unit controls the second conductive member driving device to drive the second conductive member 24 to move to the first station;
step three: the centrifugal disc position adjusting device adjusts the position of the centrifugal disc 22 so that the first conductive piece 26 is located at the second working position;
step four: the power source energizes the heater plate through the second conductive member 24 and the first conductive member 26, which heats the chip 10.
Further, the detection device comprises a detector and a detector driving device, wherein the detector is used for detecting the reaction result of the chip 10 and transmitting the detection result to the human-computer interaction terminal, and the detector driving device can drive the detector to rotate right above the detection station 21.
The specific working flow of the whole device is as follows:
and 10, repeating the steps 1-9 when the next detection is needed.
The principle and the implementation mode of the invention are explained by applying a specific example, and the description of the embodiment is only used for helping to understand the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In view of the above, the present disclosure should not be construed as limiting the invention.
Claims (11)
1. An automatic detection device, its characterized in that: the method comprises the following steps: the device comprises a magnetic bead adsorption transfer mechanism, a chip storage mechanism, a centrifugal heating mechanism, a sampling bottle and a control unit; the control unit controls the magnetic bead adsorption and transfer mechanism, the centrifugal heating mechanism and the chip transfer mechanism to work;
the sampling bottle is used for containing magnetic beads with adsorbed nucleic acid, a plurality of chips used for detecting microorganisms are stored in the chip storage mechanism, the magnetic beads are adsorbed and transferred to the magnetic beads in the sampling bottle, the sample port of the chip is stored in the chip storage mechanism, a reaction liquid is added in the chip in advance, the magnetic beads are adsorbed and transferred to the reaction liquid at the sample port of the chip, the magnetic beads are eluted by the reaction liquid, the chip transfer mechanism is used for transferring the chip containing the reaction liquid and the nucleic acid to the centrifugal heating mechanism, and the centrifugal heating mechanism is used for sequentially centrifuging and heating the chip.
2. The automatic detection device according to claim 1, characterized in that: the chip storage mechanism comprises a storage cavity and two first limiting plates, the top of the storage cavity is open, an elastic supporting piece is arranged in the storage cavity, the first limiting plate is fixedly arranged above the top of the top opening of the storage cavity, the distance between the first limiting plate and the top opening of the storage cavity is not less than the thickness value of one chip, a plurality of chips are stacked between the elastic supporting piece and the first limiting plate, the elastic supporting piece is in a compression state, a sample adding port of each chip faces upwards, the inner side wall of the storage cavity is used for limiting the horizontal movement freedom degree of the chips in the storage cavity, the two first limiting plates are respectively pressed on two opposite sides of the chips, an interval extending along a first straight line direction is arranged between the two first limiting plates, one side of a region between the top opening of the storage cavity and the first limiting plate is a chip outlet, and a push strip vertical to the first straight line is fixedly arranged on the upper surface of the chip.
3. The automatic detection device according to claim 2, characterized in that: be provided with detection station in the centrifugal heating mechanism, detection station is located the chip is stored the mechanism and is equipped with one side of chip export, chip shifts the mechanism and includes push pedal and push pedal drive arrangement, push pedal drive arrangement is used for the drive the push pedal is followed first linear motion, the acquiescence position of push pedal is located it keeps away from to store the chamber one side of detection station, the push pedal is followed first linear motion can promote the top during the direction of detection station removes push away the strip on the chip and will the chip pushes away to on the detection station.
4. The automatic detection device according to claim 3, characterized in that: chip shifts mechanism still includes the fixed plate, the vertical setting of fixed plate just the fixed plate with push pedal drive arrangement's drive end fixed connection, the top of push pedal rotate connect in the bottom of fixed plate, the push pedal orientation the one side of detection station is the front, and the another side is the back, the back side of push pedal is provided with prevents the flitch, just prevent flitch fixed connection in on the fixed plate, prevent that the flitch is used for the restriction the push pedal rotates to the back side, the push pedal can rotate to the front side when receiving the effort to the front side.
5. The automatic detection device according to claim 3, characterized in that: the magnetic bead adsorption transfer mechanism comprises a magnetic needle driving device, a magnetic needle sleeve storage device and a magnetic needle sleeve waste barrel; the sampling bottle top is uncovered, magnetism needle sleeve pipe strorage device is used for depositing the magnetism needle sleeve pipe, magnetism needle drive arrangement can drive the magnetism needle gets into magnetism needle sleeve pipe strorage device cover is established the magnetism needle sleeve pipe, and the drive the magnetism needle and the magnetism needle sleeve pipe stretches into adsorb in the sampling bottle the magnetic bead, then the drive magnetism needle sleeve pipe removes to the sample loading port department of chip is right nucleic acid on the magnetic bead elutes, drives at last the magnetism needle sleeve pipe removes to in the abandonment bucket of magnetism needle sleeve pipe will the magnetism needle sleeve pipe is abandoned.
6. The automatic detection device according to claim 5, characterized in that: the magnetic needle can be inserted into the magnetic needle sleeve, one end of the magnetic needle, which extends into the magnetic needle sleeve, is a bottom end, the other end of the magnetic needle is a top end, a first protrusion extending along the circumferential direction is arranged on the outer wall of the magnetic needle, which is close to the top end, a groove extending along the circumferential direction is arranged on the inner wall of the magnetic needle sleeve, which is close to the top end, the magnetic needle sleeve is made of an elastic material, when the magnetic needle is inserted into the magnetic needle sleeve, the first protrusion can be clamped in the groove, and a limit ring extending in the direction away from the magnetic needle sleeve is fixedly sleeved on the outer wall of the magnetic needle sleeve;
the magnetic needle sleeve storage device is provided with a plurality of magnetic needle sleeve storage stations which are cavities with top openings, one of the cavities is vertically placed in each storage station, the top opening of each magnetic needle sleeve faces upwards, and the magnetic needle driving device can drive the bottom end of each magnetic needle to vertically extend into the magnetic needle sleeves until the first bulges are clamped in the grooves.
7. The automatic detection device according to claim 6, characterized in that: the magnetic bead adsorption transfer mechanism also comprises a magnetic needle sleeve storage device driving device and a magnetic needle sleeve waste barrel driving device; the magnetic needle sleeve abandoning barrel is internally provided with at least one abandoning station which is a cavity with an opening at the top, the magnetic needle driving device can drive the magnetic needle sleeved with the magnetic needle sleeve to move to the position right above the abandoning station and drive the magnetic needle to drive a limiting ring on the magnetic needle sleeve to enter the abandoning station, and a second limiting plate is fixedly arranged at a part of an opening of the abandoning station;
the limiting ring on the magnetic needle sleeve is located when abandoning the station, magnetic needle sleeve abandonment bucket drive arrangement can drive magnetic needle sleeve abandonment bucket removes extremely the second limiting plate is in directly over the limiting ring, at this moment, the drive of magnetic needle drive arrangement the magnetic needle court when abandoning the station and moving outward, the second limiting plate prevents the limiting ring makes the magnetic needle sleeve pipe is followed drop on the magnetic needle.
8. The automatic detection device according to claim 7, characterized in that: the plurality of storage stations are uniformly distributed around a first circumference, the magnetic needle sleeve storage device driving device can drive the magnetic needle sleeve storage device to rotate around a first vertical shaft, and the first vertical shaft passes through the circle center of the first circumference;
a plurality of discarding stations are arranged in the magnetic needle sleeve waste barrel and are distributed along a second circumference, the discarding stations extend along the second circumference, the magnetic needle sleeve waste barrel driving device can drive the magnetic needle sleeve waste barrel to rotate around a second vertical shaft, and the second vertical shaft passes through the circle center of the second circumference;
the sampling bottle with the chip stores the mechanism top the orthographic projection of the sample loading port of chip on a horizontal plane is located the third circumference, arbitrary abandon the station and arbitrary deposit the station and be in orthographic projection on the horizontal plane also can be located on the third circumference, magnetic needle drive arrangement can pass through the rotating electrical machines drive the magnetic needle around the center pin of third circumference rotates.
9. The automatic detection device according to claim 8, characterized in that: the disposal station comprises a limiting cavity and an insertion cavity, the insertion cavity and the limiting cavity both extend along the second circumference, the inserting cavity and the limiting cavity are sequentially distributed along the second circumference, the inserting cavity is communicated with the limiting cavity, two opposite side walls of the limiting cavity are respectively provided with a limiting groove, the two limiting grooves respectively extend along a fourth circumference and a fifth circumference, the fourth circumference and the fifth circumference are concentric with the second circumference, the stop collar is capable of extending into the insertion cavity, when on the magnetic needle sleeve pipe the spacing ring with the spacing groove is right-time, rotate around the vertical axle of second magnetic needle sleeve pipe abandonment bucket can make the magnetic needle sleeve pipe gets into spacing chamber and the spacing ring gets into the spacing inslot, directly over the spacing groove the chamber wall structure in spacing chamber is promptly the second limiting plate.
10. The automatic detection device according to claim 5, characterized in that: the centrifugal heating mechanism comprises a centrifugal disc, a centrifugal disc driving device, a heating plate, a first conductive piece, a second conductive piece driving device and a centrifugal disc position adjusting device, the control unit controls the centrifugal disc driving device, the second conductive piece driving device and the centrifugal disc position adjusting device to work, a detection station for testing a chip is arranged on the centrifugal disc, the heating plate is embedded and fixed in the centrifugal disc, the first conductive piece is arranged on the side edge of the centrifugal disc and is electrically connected with the heating plate, the centrifugal disc driving device is used for driving the centrifugal disc to carry out centrifugal treatment on the chip, and the heating plate is used for heating the chip in the detection station; the default position of the second conductive piece is far away from the first conductive piece, the second conductive piece driving device can drive the second conductive piece to a first station, the centrifugal disc position adjusting device can adjust the position of the centrifugal disc so that the first conductive piece is located at a second station, the second conductive piece located at the first station is in contact with the first conductive piece located at the second station, and the second conductive piece is electrically connected with a power supply.
11. The automatic detection device according to claim 1, characterized in that: the detection device is used for detecting the reaction result of the chip and transmitting the detection result to the human-computer interaction terminal, and the detector driving device can drive the detector to rotate right above the detection station.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210707654.2A CN114774263B (en) | 2022-06-22 | 2022-06-22 | Automatic detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210707654.2A CN114774263B (en) | 2022-06-22 | 2022-06-22 | Automatic detection device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114774263A true CN114774263A (en) | 2022-07-22 |
| CN114774263B CN114774263B (en) | 2022-09-09 |
Family
ID=82421765
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210707654.2A Active CN114774263B (en) | 2022-06-22 | 2022-06-22 | Automatic detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114774263B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115161188A (en) * | 2022-09-07 | 2022-10-11 | 至美时代生物智能科技(北京)有限公司 | Air microorganism detection system and method |
| CN115161192A (en) * | 2022-09-07 | 2022-10-11 | 至美时代生物智能科技(北京)有限公司 | Automatic sampling device and air microorganism sampling method |
| CN115161173A (en) * | 2022-09-07 | 2022-10-11 | 至美时代生物智能科技(北京)有限公司 | Magnetic rod sleeve, sampling pipe structure and air microorganism sampling method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103923163A (en) * | 2013-01-15 | 2014-07-16 | 北京金麦格生物技术有限公司 | Workbench system and the method for bioactive substance extractor |
| CN107102131A (en) * | 2017-07-03 | 2017-08-29 | 沈阳微流控生物科技有限公司 | A kind of full-automatic micro-fluidic chip fluorescence immunoassay detecting system and its detection method |
| CN109856417A (en) * | 2019-04-04 | 2019-06-07 | 安图实验仪器(郑州)有限公司 | New type auto micro sample-adding device |
| US20210322980A1 (en) * | 2020-04-18 | 2021-10-21 | Capitalbio Corporation | Whole-process biological detection device |
| CN114364811A (en) * | 2019-09-20 | 2022-04-15 | 美国西门子医学诊断股份有限公司 | Sample preparation apparatus with PCR chip and multi-well plate |
-
2022
- 2022-06-22 CN CN202210707654.2A patent/CN114774263B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103923163A (en) * | 2013-01-15 | 2014-07-16 | 北京金麦格生物技术有限公司 | Workbench system and the method for bioactive substance extractor |
| CN107102131A (en) * | 2017-07-03 | 2017-08-29 | 沈阳微流控生物科技有限公司 | A kind of full-automatic micro-fluidic chip fluorescence immunoassay detecting system and its detection method |
| CN109856417A (en) * | 2019-04-04 | 2019-06-07 | 安图实验仪器(郑州)有限公司 | New type auto micro sample-adding device |
| CN114364811A (en) * | 2019-09-20 | 2022-04-15 | 美国西门子医学诊断股份有限公司 | Sample preparation apparatus with PCR chip and multi-well plate |
| US20210322980A1 (en) * | 2020-04-18 | 2021-10-21 | Capitalbio Corporation | Whole-process biological detection device |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115161188A (en) * | 2022-09-07 | 2022-10-11 | 至美时代生物智能科技(北京)有限公司 | Air microorganism detection system and method |
| CN115161192A (en) * | 2022-09-07 | 2022-10-11 | 至美时代生物智能科技(北京)有限公司 | Automatic sampling device and air microorganism sampling method |
| CN115161173A (en) * | 2022-09-07 | 2022-10-11 | 至美时代生物智能科技(北京)有限公司 | Magnetic rod sleeve, sampling pipe structure and air microorganism sampling method |
| CN115161173B (en) * | 2022-09-07 | 2022-12-20 | 至美时代生物智能科技(北京)有限公司 | Magnetic rod sleeve, sampling pipe structure and air microorganism sampling method |
| CN115161192B (en) * | 2022-09-07 | 2022-12-20 | 至美时代生物智能科技(北京)有限公司 | Automatic sampling device and air microorganism sampling method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114774263B (en) | 2022-09-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN114774263B (en) | Automatic detection device | |
| CN114774265B (en) | Automatic air microorganism detection system | |
| CN114774264B (en) | Automatic air microorganism detection system | |
| CN101726618B (en) | Clamping equipment and cover opener | |
| CN110501514B (en) | Automatic detector and automatic detection system | |
| CN105081768A (en) | Automatic dropping bottle assembling machine | |
| CN111495696B (en) | Inductance point gum machine | |
| CN108544228A (en) | A kind of roller bearing automatic assembling machine | |
| CN211043416U (en) | Automatic detector and automatic detection system | |
| CN113371990B (en) | Automatic cutting and extracting system for carbon absorption tube for air quality detection | |
| CN209157587U (en) | Tee tube Full-automatic assembling machine | |
| CN110305773B (en) | Nucleic acid extraction device and extraction method | |
| CN114574349B (en) | Equipment for adapting to card box | |
| WO2023011585A1 (en) | Cartridge for handling samples in nucleic acid detection, cartridge handling assembly and method of handling samples in nucleic acid detection | |
| CN210593561U (en) | Garbage containing device | |
| CN108008140B (en) | In-vitro diagnosis analysis system | |
| CN217931599U (en) | Online full automated inspection equipment of ampoule | |
| CN117823505B (en) | Optical glass electric heating lens bonding equipment and production process | |
| CN217628391U (en) | Equipment that adaptation card box used | |
| CN113089552B (en) | Lifting device of garbage sweeper and using method thereof | |
| CN217717795U (en) | Sample sucking module of immunoassay detection equipment | |
| CN215794849U (en) | Novel aseptic bag full-automatic capper | |
| CN218567393U (en) | Reaction cup and sample transfer device | |
| CN114425688B (en) | Full-automatic lamp holder locking closure machine | |
| CN217717185U (en) | Blood sample diluting device for immunoassay detection equipment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |