CN115154373B

CN115154373B - Anti-aging composition and preparation method thereof

Info

Publication number: CN115154373B
Application number: CN202210938559.3A
Authority: CN
Inventors: 何梦莲; 刘天禧; 王景圆
Original assignee: Guangzhou Ximu Biotechnology Co ltd
Current assignee: Guangzhou Ximu Biotechnology Co ltd
Priority date: 2022-08-05
Filing date: 2022-08-05
Publication date: 2023-03-21
Anticipated expiration: 2042-08-05
Also published as: CN115154373A

Abstract

The application relates to the field of cosmetics, and particularly discloses an anti-aging composition which comprises the following components in percentage by mass: 80-90% of isosorbide dimethyl ether; 8-11% of hydroxy pinacolone retinoic acid ester; glycolipid 0.5-2%; 2-5% of olive fruit oil. The application also discloses a preparation method of the anti-aging composition, which comprises the following steps: s1: respectively weighing isosorbide dimethyl ether and hydroxy pinacolone retinoic acid ester according to a formula, adding the isosorbide dimethyl ether and the hydroxy pinacolone retinoic acid ester into a stirrer, heating to 65-70 ℃, and uniformly mixing to obtain a premixed solution; s2: cooling to 40-45 deg.C, adding glycolipid and olive fruit oil into the stirrer at a stirring rate of 250-350r/min, stirring, and filtering to obtain antiaging composition. The application has the effects that the anti-wrinkle raw material has high stability and does not have irritation to skin.

Description

Anti-aging composition and preparation method thereof

Technical Field

The application relates to the field of cosmetics, in particular to an anti-aging composition and a preparation method thereof.

Background

Vitamin a, the chemical name of which is retinol, is the earliest vitamin found. There are two kinds of vitamin A. One is retinol, which is the original form of vitamin a (present only in animal foods); the other is carotene, which is converted into vitamin A pre-formed material in vivo and can be taken in from plant and animal food; the vitamin A has 3 dosage units including USP unit, IU unit, RE unit, etc.

At present, retinoids are emerging materials for removing acne and resisting wrinkle in recent years, including retinol, retinoic acid, retinal, retinol ester and the like, and the retinoids can improve abnormal keratinization and sebum excretion of epithelial cells and are clinically used as drugs for removing acne and resisting wrinkle. Although retinol is an essential active substance for the human body and is converted into retinoic acid in vivo, retinol is poor in stability, easily oxidized, and has irritation to the skin; although retinoic acid has good stability, it is highly irritating and can only be used as a prescription; although retinal is more effective than retinol, retinal itself is less stable; retinol ester, although less irritating to the skin, has low activity. If the prior retinoids are added into skin care products, the requirement of people cannot be met. Thus, there is still room for improvement.

Disclosure of Invention

In order to enable the anti-wrinkle raw material to have high stability and no irritation to the skin, the application provides an anti-aging composition and a preparation method thereof.

In a first aspect, the present application provides an anti-aging composition, which adopts the following technical scheme:

an anti-aging composition comprises the following components in percentage by mass:

80-90% of isosorbide dimethyl ether;

8-11% of hydroxy pinacolone retinoic acid ester;

glycolipid 0.5-2%;

2-5% of olive fruit oil.

By adopting the technical scheme, the hydroxy pinacolone retinoic acid ester is a retinol derivative, can be directly combined with cell Retinoic Acid Receptors (RARs), has the functions of regulating metabolism of epidermis and stratum corneum, can resist aging, reduce seborrhea, fade epidermal pigments, prevent skin aging, prevent acne, whiten skin and lighten spots and the like; and, the irritation to the skin is very small.

Isosorbide dimethyl ether (DMI) is a colorless transparent liquid, is used as a solvent of hydroxy pinacoline retinoic acid ester, is beneficial to increasing the transfer of the hydroxy pinacoline retinoic acid ester on the surface layer of the skin, has the functions of helping osmosis, promoting skin absorption and having small irritation to the skin; moreover, the dimethyl isosorbide can reduce the viscosity of the system and is used as a viscosity control agent. Meanwhile, the dimethyl isosorbide can reduce the concentration of hydroxy pinacolone retinoic acid ester so as to achieve the best anti-aging effect.

Glycolipids belong to lipid compounds, which are widely present in various organisms, and glycolipids in nature can be classified into two major groups according to the kinds of alcohol groups in their components: glyceroglycolipids and glycosphingolipids. The glycolipid is a water-soluble lipid substance, has a structure similar to that of a substance forming the horny layer of the skin, can quickly permeate into the skin and combine with water in the horny layer to form a net structure, locks water and has a good moisturizing effect. Under the action of glycolipid, the hydroxy pinatone retinoic acid ester can penetrate into skin more quickly, so that a faster anti-wrinkle effect is achieved.

The olive fruit oil is a natural skin-moistening oil, has fragrant and elegant fragrance, can deeply nourish the skin, and has excellent ductility on the skin. The glycolipid belongs to an amphiphilic substance (namely, hydrophilic and lipophilic), and can wrap the olive fruit oil in a network structure, so that the olive fruit oil is better dispersed in the system.

According to the application, the hydroxy pinacolone retinoic acid ester, the isosorbide dimethyl ether, the glycolipid and the olive fruit oil are matched in a synergistic mode, so that the anti-aging composition has high stability and low irritation to skin, and the anti-aging composition can meet the requirements of people when being added into a skin care product.

Preferably, the anti-aging composition comprises the following components in percentage by mass:

84.5 to 85.5 percent of isosorbide dimethyl ether;

9.5 to 10.5 percent of hydroxy pinacolone retinoic acid ester;

glycolipid 0.5-1.5%;

olive fruit oil 3.5-4.5%.

By adopting the technical scheme, the anti-wrinkle effect of the anti-aging composition is better, and the irritation of the anti-aging composition to skin is further reduced.

Preferably, the preparation method of the olive fruit oil comprises the following steps:

inoculating the Candida utilis subjected to the secondary culture to a fermentation tank by using the inoculation amount of 8-10%, adding olive fruits to the fermentation tank, and fermenting at 25-30 ℃ for 30-35h; heating to 65-70 deg.C to inactivate Candida, centrifuging to obtain oil phase, and vacuum dewatering to obtain olive fruit oil.

By adopting the technical scheme, the olive fruit oil prepared by the method has higher activity, so that squalene rich in olive oil and fatty acid required by a human body can be quickly absorbed by the skin, and the elasticity and luster of the skin are effectively kept; meanwhile, the vitamin E, the vitamin K, the vitamin A, the vitamin D and the like contained in the olive fruit oil and the phenolic antioxidant substances have the effects of eliminating skin wrinkles, delaying senility, preventing skin chap and resisting ultraviolet rays. And, the irritation of hydroxyppinacolone retinoic acid ester to the skin is further reduced because olive fruit oil produces a substance against the irritation of hydroxyppinacolone retinoic acid ester during fermentation, thereby reducing the irritation of hydroxyppinacolone retinoic acid ester to the skin.

In a second aspect, the present application provides a method for preparing an anti-aging composition, which adopts the following technical scheme:

a method for preparing an anti-aging composition, comprising the following steps:

s1: respectively weighing isosorbide dimethyl ether and hydroxy pinacolone retinoic acid ester according to the formula, sequentially adding into a stirrer, heating to 65-70 ℃, and uniformly mixing to obtain a premixed solution;

s2: cooling to 40-45 deg.C, adding glycolipid and olive fruit oil into the stirrer at a stirring rate of 250-350r/min, stirring, and filtering to obtain antiaging composition.

By adopting the technical scheme, the anti-aging composition prepared by the method has high stability, and the glycolipid mainly comprises alcohol, fat and sugar, so that a system consisting of the glycolipid, the olive oil, the isosorbide dimethyl ether and the hydroxy pinacolone retinoic acid ester belongs to an anhydrous emulsifying system, the stability of an anhydrous emulsion is poorer than that of an aqueous emulsion, and the anti-aging composition prepared by the method has high stability, and the phenomenon of demulsification is not easy to occur in the high-speed stirring process of the anhydrous emulsion, so that the anti-wrinkle effect of the anti-aging composition is not easy to influence.

Preferably, the mixer includes the base and installs the churn on the base, the one end that the base was kept away from to the churn is equipped with the feed inlet and is used for the lid to close churn open-ended kettle cover, be equipped with the stirring rake on the kettle cover and be used for ordering about stirring rake pivoted motor, the stirring rake is including being fixed in the (mixing) shaft on the motor, the lateral wall of (mixing) shaft is equipped with a plurality of horizontal stirring leaves and a plurality of vertical stirring leaf, horizontal stirring leaf includes a plurality of blades, the one end that the (mixing) shaft was kept away from to the blade is equipped with a plurality of bristles, the one end that the (mixing) shaft was kept away from to the bristle is with the inside wall butt of churn.

Through adopting above-mentioned technical scheme, when hydroxyl pinacolone retinoic acid ester powder gets into the churn from the feed inlet in, because partial powder adheres easily on the inside wall of churn, the setting of brush hair can be brushed the powder that adheres on the churn inside wall and fall to in the isosorbide dimethyl ether solution to this makes isosorbide dimethyl ether and hydroxyl pinacolone retinoic acid ester powder mix ground more evenly.

Preferably, a plurality of circular rings are arranged on the blade and are uniformly distributed along the horizontal direction of the blade.

By adopting the technical scheme, the impact area of the anhydrous emulsion and the blades is reduced, and the impact force generated by the collision of the anhydrous emulsion and the blades during high-speed rotation is favorably reduced, so that the anhydrous emulsion is not easy to break emulsion, and the anti-wrinkle effect of the anti-aging composition is not easy to be influenced. In addition, in the process of high-speed stirring, the original motion track of the powder falling from the brush bristles through the plurality of circular rings can be changed, and the powder moves from one end close to the inner side wall of the stirring cylinder to the direction close to the stirring shaft, so that the powder can be fully and uniformly mixed in the horizontal direction.

Preferably, one end of the longitudinal stirring blade, which is far away from the stirring shaft, is obliquely arranged towards the bottom of the stirring barrel.

Through adopting above-mentioned technical scheme for the powder of churn bottom can upwards roll along the vertical stirring leaf of slope, makes the powder misce bene of churn bottom more fast with this, is favorable to improving stirring efficiency.

Preferably, a plurality of longitudinal stirring blades are staggered and uniformly distributed along the circumferential direction of the stirring shaft.

Through adopting above-mentioned technical scheme for the powder of churn bottom can mix more evenly in the depth direction.

Preferably, the stirring paddle is made of elastic materials.

Through adopting above-mentioned technical scheme, at the stirring in-process, liquid can constantly pat on the stirring rake, because the stirring rake has the cushioning effect for anhydrous emulsion is difficult to appear the phenomenon of breakdown of emulsion, and then makes the system more stable, makes the crease-resistant effect of antidetonation composition be difficult to receive the influence.

Preferably, the bristles are made of polytetrafluoroethylene material.

By adopting the technical scheme, because the surface tension of the polytetrafluoroethylene is very small and any substance is not easy to adhere to the polytetrafluoroethylene, the bristles made of the polytetrafluoroethylene material are not easy to adhere to the anhydrous emulsion, so that the anti-aging composition is not easy to lose quality in the preparation process, and the preparation of the anti-aging composition is not easy to be influenced.

In summary, the present application includes at least one of the following beneficial technical effects:

1. by adopting the synergistic cooperation of the hydroxy pinacolone retinoic acid ester, the isosorbide dimethyl ether, the glycolipid and the olive fruit oil, the anti-aging composition has higher stability and low irritation to skin, so that the anti-aging composition can meet the requirements of people when being added into a skin care product.

2. The olive fruit oil prepared by the method has high activity, so that squalene rich in olive oil and fatty acid required by a human body can be quickly absorbed by the skin, and the elasticity and luster of the skin are effectively kept; meanwhile, the vitamin E, K, A, D and the like contained in the olive fruit oil and the phenolic antioxidant substances have the effects of eliminating skin wrinkles, delaying senility, preventing skin chapping and resisting ultraviolet rays; and, since the olive fruit oil produces a substance against irritation of hydroxyppinacolone retinoic acid ester during fermentation, irritation of hydroxyppinacolone retinoic acid ester to skin is reduced.

3. Through the arrangement of the bristles, the powder adhered to the inner side wall of the stirring cylinder can be brushed down into the isosorbide dimethyl ether solution, so that the isosorbide dimethyl ether and the hydroxy pinacolone retinoic acid ester powder are mixed more uniformly; and the original movement track can be changed through a plurality of rings to the powder that the brush hair brush fell, moves from the one end that is close to the churn inside wall towards the direction that is close to the (mixing) shaft for the powder can the intensive mixing in the horizontal direction evenly.

4. Through the arrangement of the plurality of rings, the impact area between the anhydrous emulsion and the blades is reduced, the impact force generated by the collision between the anhydrous emulsion and the blades during high-speed rotation is favorably reduced, the demulsification phenomenon of the anhydrous emulsion is not easy to occur, and the anti-wrinkle effect of the anti-aging composition is not easy to be influenced.

Drawings

FIG. 1 is a schematic diagram of a blender specifically adapted for use in preparing an anti-aging composition according to the present application.

Fig. 2 is a schematic view of the internal structure of a blender for preparing an anti-aging composition.

In the figure: 1. a base; 2. a mixing drum; 21. a feed inlet; 22. a kettle cover; 23. a motor; 3. a stirring paddle; 31. a stirring shaft; 32. a transverse stirring blade; 321. a blade; 322. brushing; 323. a circular ring; 33. and (4) longitudinally stirring the blades.

Detailed Description

The present application will be described in further detail with reference to examples.

Preparation 1

The preparation method of the olive fruit oil comprises the following steps:

inoculating the Candida utilis subjected to the secondary culture into a fermentation tank by using the inoculation amount of 8%, adding 10kg of olive fruits into the fermentation tank, and fermenting for 30 hours at 25 ℃; heating to 65 ℃ to inactivate candida, carrying out centrifugal separation to obtain an oil phase, and then carrying out vacuum dehydration on the oil phase to obtain the olive fruit oil.

Preparation 2

The difference from preparation 1 is that:

inoculating Candida utilis subjected to secondary culture into a fermentation tank with the inoculation amount of 10%, adding 10kg of olive fruits into the fermentation tank, and fermenting at 30 ℃ for 35h; heating to 70 ℃ to inactivate candida, carrying out centrifugal separation to obtain an oil phase, and then carrying out vacuum dehydration on the oil phase to obtain the olive fruit oil.

Example 1

The embodiment discloses an anti-aging composition, which comprises the following components:

isosorbide dimethyl ether; hydroxy pinacolone retinoic acid ester; glycolipids; olive fruit oil;

wherein, the glycolipid is sophorolipid, which is sold by Shanxi Yikang dragon biotechnology limited company; the isosorbide dimethyl ether is obtained by the method sold by Shandong Yuanhua Biotechnology Limited, and the purity is more than 99 percent; the hydroxy pinacolone retinoic acid ester is sold by bioscience of east west Jun Chi, and has a purity of more than 98%.

The embodiment also discloses a preparation method of the anti-aging composition, which comprises the following steps:

s1: respectively weighing isosorbide dimethyl ether and hydroxy pinacolone retinoic acid ester according to a formula (the specific dosage is shown in table 1), sequentially adding into a stirrer, heating to 65 ℃, stirring at a speed of 180r/min, and uniformly mixing to obtain a premixed solution;

s2: cooling to 40 deg.C, adding glycolipid and olive fruit oil (specific dosage shown in Table 1) into the stirrer at a stirring rate of 250r/min, stirring, and filtering with 500 mesh gauze to obtain antiaging composition; cooling to room temperature, inspecting, discharging, standing, inspecting, filling, and storing.

Referring to fig. 1, the mixer includes a base 1 and a mixing drum 2 fixed on the base 1, the mixing drum 2 is cylindrical, a feed inlet 21 is opened at one end of the mixing drum 2 far from the base 1, and the raw materials are fed into the mixer from the feed inlet 21.

Referring to fig. 2, the one end that base 1 was kept away from to churn 2 is articulated to have and is used for the lid to close 2 open-ended kettle lids 22 of churn, be equipped with stirring rake 3 on the kettle lid 22 and be used for driving about 3 pivoted motors 23 of stirring rake, stirring rake 3 adopts the rubber material to make, has the cushioning effect for the phenomenon of breakdown of emulsion is difficult to appear at the stirring in-process to anhydrous emulsion, and then makes the system more stable, makes the crease-resistant effect of anti-ageing composition be difficult to receive the influence.

Referring to fig. 2, the stirring paddle 3 includes a stirring shaft 31 fixed to the motor 23, the outer side wall of the stirring shaft 31 is provided with two transverse stirring blades 32, each transverse stirring blade 32 includes three blades 321, and the three blades 321 are uniformly distributed along the circumferential direction of the stirring shaft 31. The end of the blade 321 far away from the stirring shaft 31 is fixed with a plurality of bristles 322, and the end of the bristles 322 far away from the stirring shaft 31 is abutted against the inner side wall of the stirring cylinder 2. When the hydroxy pinacolone retinoic acid ester powder is fed into the stirring cylinder 2 from the feeding hole 21, the brush bristles 322 can brush the powder adhered to the inner side wall of the stirring cylinder 2 into the isosorbide dimethyl ether solution in the stirring process, so that the isosorbide dimethyl ether and the hydroxy pinacolone retinoic acid ester are stirred more fully. The bristles 322 are made of a teflon material so that the anhydrous emulsion is not easily adhered to the bristles 322, thereby making the antiaging composition less likely to suffer from a loss of quality during the preparation process.

Referring to fig. 2, each blade 321 is provided with a plurality of rings 323, in this embodiment, four rings 323 are provided on each blade 321, and the four rings 323 are uniformly distributed along the horizontal direction of the blade 321, which not only reduces the resistance of the transverse stirring blade 32 in the rotation process, so that the anhydrous emulsion can be mixed more uniformly, but also reduces the impact area between the anhydrous emulsion and the transverse stirring blade 32, which is beneficial to reducing the impact force generated by the collision between the anhydrous emulsion and the transverse stirring blade 32 during the high-speed rotation, so that the anhydrous emulsion is not easy to break. Meanwhile, in the process of high-speed stirring, the powder brushed down by the brush bristles 322 passes through the four circular rings 323 to change the original motion track, and moves from one end close to the inner side wall of the stirring cylinder 2 to the direction close to the stirring shaft 31, so that the powder can be fully and uniformly mixed in the horizontal direction.

Referring to fig. 2, the outer side wall of the stirring shaft 31 is further provided with a longitudinal stirring blade 33, the longitudinal stirring blade 33 is located between two transverse stirring blades 32, and the longitudinal stirring blade 33 is provided with a plurality of blades. In the present embodiment, nine longitudinal stirring vanes 33 are provided, nine longitudinal stirring vanes 33 are uniformly distributed along the length direction of the stirring shaft 31, and the nine longitudinal stirring vanes 33 are staggered along the circumferential direction of the stirring shaft 31. One end of the longitudinal stirring blade 33, which is far away from the stirring shaft 31, is obliquely arranged towards the bottom of the stirring cylinder 2, so that the materials at the bottom roll upwards better along the oblique longitudinal stirring blade 33, and the circulation and the mixing in the depth direction are carried out, so that the anhydrous emulsion can be fully and uniformly mixed in the vertical direction.

Example 2

The difference from example 1 is that: the amounts of the components used are different, see in particular table 1.

S1: respectively weighing isosorbide dimethyl ether and hydroxy pinacolone retinoic acid ester according to a formula (the specific dosage is shown in table 1), then adding the weighed materials into a stirrer, heating the materials to 70 ℃, and uniformly mixing the materials at a stirring speed of 180r/min to obtain a premixed solution;

s2: cooling to 45 deg.C, adding glycolipid and olive fruit oil (specific dosage shown in Table 1) into the stirrer at a stirring rate of 350r/min, stirring, and filtering with 500 mesh gauze to obtain antiaging composition; cooling to room temperature, inspecting, discharging, standing, inspecting, filling, and storing.

Examples 3 to 4

The difference from example 1 is that: the amount of each component is different, specifically referring to table 1, and the unit of the amount of each component in table 1 is kg.

TABLE 1

Example 5

The differences from example 4 are: the olive fruit oil is the olive fruit oil prepared in the preparation 1.

Example 6

The difference from example 4 is that: the olive fruit oil is the olive fruit oil prepared in the preparation 2.

Example 7

The difference from example 4 is that: the stirring paddle in the stirrer is made of stainless steel.

Example 8

The difference from example 4 is that: the stirring paddle in the stirrer adopts a spiral stirring blade.

Comparative example 1

The difference from example 4 is that: equal amount of dipropylene glycol was substituted for dimethyl isosorbide anhydride.

Comparative example 2

The difference from example 4 is that: the hydroxy pinacolone retinoic acid ester is replaced by an equal amount of retinol palmitate.

Comparative example 3

The difference from example 4 is that: equal amount of mannoerythritol lipid was substituted for sophorolipid.

Comparative example 4

The difference from example 4 is that: the olive fruit oil is replaced by the same amount of shea butter.

Comparative example 5

The difference from example 4 is that:

an anti-aging composition comprises the following components in parts by mass:

74kg of isosorbide dimethyl ether; 5kg of hydroxy pinacolone retinoic acid ester; glycolipid 3kg; 1kg of olive fruit oil.

Comparative example 6

The difference from example 4 is that:

an anti-aging composition comprises the following components in parts by mass:

94kg of isosorbide dimethyl ether; 14kg of hydroxy pinacolone retinoic acid ester; glycolipid 0.3kg; 7kg of olive fruit oil.

Application example 1

The anti-aging compositions prepared in the above examples and comparative examples are respectively added into anti-aging emulsions, and the anti-aging emulsions comprise the following components by mass:

0.05kg of 2-hydroxy-4-methoxy-5-sulfobenzophenone; 26 12kg of glycerol polyether; polysorbate-80.5kg; 0.2kg of phenoxyethanol; 1.5kg of stearic acid; 0.3kg of tocopherol acetate; 1.1kg of polydimethylsiloxane; 0.22kg of EDTA disodium; 5.9kg of Potentilla tormentosa root extract; 5.4kg of white Potentilla chinensis seed oil; 1kg of anti-aging composition; 71.83kg of water.

The preparation method of the anti-aging emulsion comprises the following steps:

s1: mixing stearic acid and white Potentilla seed oil, stirring, heating to 80 deg.C, and maintaining the temperature for 30min to obtain mixture A;

s2: cooling to 70 ℃, adding water, glyceryl polyether-26, polydimethylsiloxane, tocopheryl acetate and polysorbate-80 into the mixture A, and uniformly stirring to obtain a mixture B;

s3: continuously cooling to 40 ℃, and respectively adding the anti-aging composition, 2-hydroxy-4-methoxy-5-benzophenone sulfonate, disodium EDTA, potentilla chinensis root extract and phenoxyethanol into the mixture B, and uniformly stirring to obtain an anti-aging emulsion;

the anti-aging composition prepared in example 1 was used.

Application example 2

The difference from application example 1 is that:

anti-aging composition the anti-aging composition prepared in example 2 was used.

Application example 3

The difference from application example 1 is that:

anti-aging composition the anti-aging composition prepared in example 3 was used.

Application example 4

The difference from application example 1 is that:

anti-aging composition the anti-aging composition prepared in example 4 was used.

Application example 5

The difference from application example 4 is that:

anti-aging composition the anti-aging composition prepared in example 5 was used.

Application example 6

The difference from application example 4 is that:

anti-aging composition the anti-aging composition prepared in example 6 was used.

Application example 7

The difference from application example 4 is that:

anti-aging composition the anti-aging composition prepared in example 7 was used.

Application example 8

The difference from application example 4 is that:

anti-aging composition the anti-aging composition prepared in example 8 was used.

Comparative application example 1

The differences from application example 4 are:

anti-aging composition the anti-aging composition prepared in comparative example 1 was used.

Comparative application example 2

The differences from application example 4 are:

anti-aging composition the anti-aging composition prepared in comparative example 2 was used.

Comparative application example 3

The differences from application example 4 are:

anti-aging composition the anti-aging composition prepared in comparative example 3 was used.

Comparative application example 4

The difference from application example 4 is that:

anti-aging composition the anti-aging composition prepared in comparative example 4 was used.

Comparative application example 5

The difference from application example 4 is that:

anti-aging composition the anti-aging composition prepared in comparative example 5 was used.

Comparative application example 6

The difference from application example 4 is that:

anti-aging composition the anti-aging composition prepared in comparative example 6 was used.

Blank control group

The differences from application example 4 are:

the anti-aging composition is not added into the anti-aging emulsion.

Experiment 1 stability test

This experiment examined the stability of the anti-aging compositions of examples 4 to 8 and comparative examples 1 to 6, respectively, and the anti-aging compositions of examples 4 to 8 and comparative examples 1 to 6 were stored at 25 ℃ and 45 ℃ for 3 months and at the light for one month, respectively, and the stability of the anti-aging compositions of examples 4 to 8 and comparative examples 1 to 6 was observed, respectively, and the results are shown in Table 2.

TABLE 2

According to the comparison of comparative examples 1-6 in table 2 with example 4, the anti-aging composition has high stability only by using the synergistic combination of hydroxy pinacolone retinoic acid ester, isosorbide dimethyl ether, glycolipid and olive fruit oil, but not by using any substance.

Experiment 2 skin irritation test

The anti-aging emulsion prepared in the application example, the comparative application example and the blank control group was subjected to a human body patch test by referring to the method described in technical specification for cosmetic safety (2015), in this experiment.

The experimental population is as follows: the total 75 persons, female 40 persons, male 35 persons, age 21-59 persons, 5 persons in each group, meet the volunteer enrollment criteria of the subject.

The test method comprises the following steps: the selection area is not more than 50mm ² Approximately 0.020mL of test substance (anti-aging emulsion) was added to a suitable plaque tester having a depth of approximately 1mm by the closed patch test method. The plaque test apparatus is applied to the curved side of the forearm of the subject, the subject is removed after 24 hours, the skin reaction is observed, the results are recorded according to the skin reaction grading standard in technical specifications for cosmetic safety (2015 edition), and the skin irritation reaction scores are shown in table 3.

TABLE 3

Experiment 3 anti-wrinkle Effect test

The experiment respectively detects the anti-wrinkle effect of the anti-aging emulsions prepared in the application examples 1 to 8 and the comparative application examples 1 to 6 and the blank control group, and the change condition of the elasticity value of the applied part is measured by a skin elasticity tester (Cutomer dual MPA 580), and each subject is measured by the same technician every time.

The test population: 150 wrinkled persons with the average age of 35-45 years old. Among them, 70 men and 80 women had no damage to skin health, and 10 subjects were used for each application example and comparative application example, and the results were averaged.

(1) Respectively taking the anti-aging emulsions prepared in the application examples 1-8, the comparative application examples 1-6 and the blank control group;

(2) Selecting the left and right cheekbones of the face of a subject, the left and right cheeks at the intersection of the nose tip and the pupil, and the left and right sides at the intersection of the temple and the mouth corner as test areas;

(3) The cosmetic is applied twice in the morning and at night every day, the picture before application is taken as a control, and the subject cannot be applied with any other cosmetics during the experiment;

(4) The subjects counted the rate of change in skin elasticity before and after the use 28 days after the continuous use of the anti-aging lotions prepared in application examples 1 to 8 and comparative application examples 1 to 6, as well as the blank control group.

The results of

experiments

2 and 3 are shown in Table 4.

TABLE 4

According to the comparison of comparative application examples 1 to 4 in table 4 with the data of application example 4, the comparative application examples 1 to 4, in which one of hydroxyppinacolone retinoic acid ester, isosorbide dimethyl ether, glycolipid and olive fruit oil was replaced, produced skin irritation, while the anti-aging composition prepared from hydroxyppinacolone retinoic acid ester, isosorbide dimethyl ether, glycolipid and olive fruit oil was added to the emulsion without any irritation to the skin. In addition, the skin elasticity change rate of application example 4 is significantly higher than that of comparative application examples 1 to 4, which shows that the anti-aging composition prepared from hydroxy pinacolone retinoic acid ester, isosorbide dimethyl ether, glycolipid and olive fruit oil has a good anti-wrinkle effect.

According to the comparison between the application example 7 and the application example 4 in table 4, the rigid material (stainless steel) is adopted in the stirring paddle in the application example 7, the skin elasticity change rate is 37%, the elastic material (rubber) is adopted in the stirring paddle in the application example 4, the skin elasticity change rate is 50%, and the skin elasticity change rate is increased by 13%, which indicates that the elastic material is adopted in the stirring paddle, so that the anti-wrinkle effect of the anti-aging composition is improved, because the liquid can continuously flap on the stirring paddle in the stirring process, and the stirring paddle has a buffering effect, the phenomenon of emulsion breaking is not easy to occur in the anhydrous emulsion, and the system is more stable, so that the anti-wrinkle effect of the anti-aging composition is not easily influenced.

According to the comparison between the application example 8 and the application example 4 in the table 4, the helical stirring blade is adopted in the stirring blade in the application example 8, the skin elasticity change rate is 33%, while the transverse stirring blade and the longitudinal stirring blade are arranged on the blade of the stirring blade in the application example 4, the skin elasticity change rate is 50%, and the skin elasticity change rate is increased by 17%, which means that the transverse stirring blade and the longitudinal stirring blade are arranged on the blade of the stirring blade, so that the anti-wrinkle effect of the anti-aging composition is improved, because the impact area of the anhydrous emulsion and the blade can be reduced in the stirring process by the transverse stirring blade and the longitudinal stirring blade, the impact force generated by the collision of the anhydrous emulsion and the blade during the high-speed rotation of the anhydrous emulsion is reduced, the demulsification phenomenon of the anhydrous emulsion is not easy to occur, and the anti-wrinkle effect of the anti-aging composition is not easy to be influenced.

According to the comparative application examples 5-6 in table 4, the anti-aging composition content is outside the range of the application, and the better anti-wrinkle effect can not be achieved.

The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims

1. An anti-aging composition characterized by: the composite material comprises the following components in percentage by mass:

80-90% of isosorbide dimethyl ether;

8-11% of hydroxy pinacolone retinoic acid ester;

glycolipid 0.5-2%;

olive fruit oil 2-5%;

the sum of the mass percentages of all the components is 100 percent;

the preparation method of the olive fruit oil comprises the following steps:

inoculating the Candida utilis subjected to the secondary culture to a fermentation tank by using the inoculation amount of 8-10%, adding olive fruits to the fermentation tank, and fermenting at 25-30 ℃ for 30-35h; heating to 65-70 deg.C to inactivate Candida, centrifuging to obtain oil phase, and vacuum dewatering to obtain olive fruit oil;

s2: cooling to 40-45 deg.C, adding glycolipid and olive fruit oil into the stirrer at a stirring rate of 250-350r/min, stirring, and filtering to obtain antiaging composition;

the stirrer comprises a base (1) and a stirring cylinder (2) installed on the base (1), wherein one end, far away from the base (1), of the stirring cylinder (2) is provided with a feed inlet (21) and is used for covering an opening kettle cover (22) of the stirring cylinder (2), the kettle cover (22) is provided with a stirring paddle (3) and a motor (23) used for driving the stirring paddle (3) to rotate, the stirring paddle (3) comprises a stirring shaft (31) fixed on the motor (23), the outer side wall of the stirring shaft (31) is provided with a plurality of transverse stirring blades (32) and a plurality of longitudinal stirring blades (33), each transverse stirring blade (32) comprises a plurality of blades (321), one end, far away from the stirring shaft (31), of each blade (321) is provided with a plurality of bristles (322), and one end, far away from the stirring shaft (31), of each bristle (322) is abutted to the inner side wall of the stirring cylinder (2);

a plurality of circular rings (323) are arranged on the blade (321), and the circular rings (323) are uniformly distributed along the horizontal direction of the blade (321);

the stirring paddle (3) is made of elastic materials.

2. An anti-ageing composition according to claim 1, wherein: the anti-aging composition comprises the following components in percentage by mass:

84.5 to 85.5 percent of isosorbide dimethyl ether;

9.5 to 10.5 percent of hydroxy pinacolone retinoic acid ester;

glycolipid 0.5-1.5%;

olive fruit oil 3.5-4.5%.

3. A process for the preparation of an anti-ageing composition according to claim 1, characterized in that: one end of the longitudinal stirring blade (33) far away from the stirring shaft (31) is obliquely arranged towards the bottom of the stirring cylinder (2).

4. A process for the preparation of an anti-ageing composition according to claim 3, characterized in that: the longitudinal stirring blades (33) are staggered and uniformly distributed along the circumferential direction of the stirring shaft (31).

5. A process for the preparation of an anti-ageing composition according to claim 1, characterized in that: the brush bristles (322) are made of polytetrafluoroethylene materials.