JP2008208053A - Composition for promoting skin cell growth and method for producing the same - Google Patents
Composition for promoting skin cell growth and method for producing the same Download PDFInfo
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- JP2008208053A JP2008208053A JP2007044766A JP2007044766A JP2008208053A JP 2008208053 A JP2008208053 A JP 2008208053A JP 2007044766 A JP2007044766 A JP 2007044766A JP 2007044766 A JP2007044766 A JP 2007044766A JP 2008208053 A JP2008208053 A JP 2008208053A
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- skin
- extract
- lipid fraction
- neutral lipid
- cell growth
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/304—Foods, ingredients or supplements having a functional effect on health having a modulation effect on allergy and risk of allergy
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
Abstract
Description
本発明は、カカオ豆外皮抽出物およびカカオ豆外皮の中性脂質画分を有効成分として含有することを特徴とする皮膚細胞増殖促進剤およびその製造方法に関する。 The present invention relates to a skin cell proliferation promoter and a method for producing the same, comprising a cacao bean hull extract and a neutral lipid fraction of a cacao bean hull as active ingredients.
皮膚の構造は最上層から表皮、真皮、皮下組織の三層に分かれている。表皮は皮膚の最も外側にあり外気や紫外線などの様々な刺激にさらされている。表皮はその大部分を表皮角化細胞（ケラチノサイト）で構成されており、表皮角化細胞の分化段階によって、外側から角質層、顆粒層、有棘層、基底層の４つの細胞層に分かれている。細胞が基底層から角質層まで成熟し、はがれ落ちるまでのサイクルをターンオーバーといい、このサイクルがスムーズに行われることにより、皮膚は一定の厚さと若さを保っている。 The structure of the skin is divided into three layers from the top layer to the epidermis, dermis, and subcutaneous tissue. The epidermis is on the outermost side of the skin and is exposed to various stimuli such as outside air and ultraviolet rays. Most of the epidermis is composed of epidermal keratinocytes (keratinocytes). The epidermis keratinocytes are divided into four cell layers from the outside, the stratum corneum, granule layer, spinous layer, and basal layer, depending on the differentiation stage. Yes. The cycle from when the cell matures from the basal layer to the stratum corneum and peels off is referred to as turnover. The smoothness of this cycle keeps the skin constant and young.
真皮は皮膚の張りや弾力性に関わるエラスチンやコラーゲン、保水性の維持に重要なヒアルロン酸等の細胞外マトリックスと真皮細胞により構成されており、真皮細胞の中で線維芽細胞が細胞外マトリックス成分を合成している。 The dermis is composed of extracellular matrix such as elastin and collagen, which are related to skin tension and elasticity, hyaluronic acid which is important for maintaining water retention, and dermal cells. Among dermal cells, fibroblasts are extracellular matrix components. Is synthesized.
加齢、紫外線暴露、空気乾燥等により、皮膚の表皮細胞および真皮細胞はダメージを受ける。表皮においては表皮角化細胞の減少と代謝の低下によりターンオーバー速度が遅くなることによって、肌は保湿性やなめらかさを失い、硬くなったりくすみや小ジワが現れたりする。更には、メラニンが過剰に生成された部位では表皮細胞の分裂、増殖が低下しメラニン排出が滞り、よりシミのできやすい状態となる。真皮においては細胞外マトリックスの主要構成成分であるエラスチンやコラーゲンの分解及び変質、線維芽細胞の増殖低下に伴う細胞外マトリックスの産生能力の低下により、肌は張りや弾力を失い、肌荒れ、シワ等を生じる。 Skin epidermal cells and dermal cells are damaged by aging, UV exposure, air drying, and the like. In the epidermis, the turnover speed becomes slow due to the decrease in epidermal keratinocytes and the decrease in metabolism, so that the skin loses moisture retention and smoothness, and the skin becomes hard, dull and wrinkles appear. Furthermore, at a site where melanin is excessively generated, the division and proliferation of epidermal cells are reduced, melanin excretion is delayed, and it becomes easier to cause spots. In the dermis, the skin loses its elasticity and elasticity due to the degradation and alteration of elastin and collagen, which are the main components of the extracellular matrix, and the decrease in the production capacity of the extracellular matrix associated with the decrease in fibroblast proliferation, rough skin, wrinkles, etc. Produce.
従って、表皮角化細胞の増殖促進により、皮膚のターンオーバーが早くなり、小ジワやくすみ、しみを軽減させる。また、真皮線維芽細胞の増殖促進により、コラーゲンやヒアルロン酸の産生を向上させ、シワの形成や肌の弾性低下を防止及び改善することができると考えられる。さらには外傷や火傷、手術等による創傷治癒の促進効果も期待される。また、重度のやけど等を負った患者等に対する治療法として、患者自身の皮膚断片を培養し、これを移植する方法がある。培養可能な皮膚組織の採取から完成まで時間を必要とすることが問題とされているが、細胞増殖促進剤の使用により皮膚細胞の培養期間短縮が期待される。 Therefore, by promoting the proliferation of epidermal keratinocytes, skin turnover is accelerated, and wrinkles, dullness, and spots are reduced. In addition, it is considered that by promoting the growth of dermal fibroblasts, the production of collagen and hyaluronic acid can be improved, and the formation of wrinkles and the decrease in skin elasticity can be prevented and improved. Furthermore, the effect of promoting wound healing by trauma, burns, surgery, etc. is also expected. As a treatment method for patients suffering from severe burns, etc., there is a method of culturing a patient's own skin fragment and transplanting it. Although it has been a problem that it takes time from collection of cultivatable skin tissue to completion, the use of a cell growth promoter is expected to shorten the culture period of skin cells.
これまで、皮膚の抗老化予防素材としてコラーゲン産生促進作用、表皮角化細胞増殖 作用を有する物質を天然物から取得しようという試みがなされている。 Until now, attempts have been made to acquire substances having an action of promoting collagen production and an action of proliferating epidermal keratinocytes from natural products as materials for preventing skin aging.
例えば、表皮細胞増殖促進組成物としてカカオマスおよびココアパウダー（特許文献１参照）が開示されているが、カカオ豆外皮に関する効果は示されていない。ケラチノサイトの分化及び角化に関与する遺伝子を活性化させる素材としてカカオ豆および外皮の水、水―アルコール抽出物（特許文献２、３参照）が開示されているが、カカオ豆外皮の低極性溶媒抽出物である脂質画分に関する効果は示されていない。皮膚の加齢症状防止組成物、化粧品としてポリフェノールを含有するココア抽出物、より詳細にはポリフェノール、アミノ酸、及びケン化され得ない物質の濃縮物を組み合わせて含有するココア抽出物（特許文献４参照）に関して開示されているが、カカオ豆外皮の低極性溶媒抽出物である脂質画分に関する細胞増殖促進効果は示されていない。また、上記のカカオ外皮由来の非ケン化性脂質画分が毒性物質ジメチルホルムアミドの添加された培養液中で線維芽細胞の増殖を部分的に保護したとの報告はあるが、通常の培養条件で増殖を促進させたとの報告はされていない（非特許文献１参照）。 For example, cocoa mass and cocoa powder (see Patent Document 1) have been disclosed as epidermal cell growth promoting compositions, but no effect on cocoa bean hulls is shown. As materials for activating genes involved in differentiation and keratinization of keratinocytes, cocoa beans and hull water and water-alcohol extracts (see Patent Documents 2 and 3) have been disclosed. No effect on the lipid fraction that is the extract is shown. Composition for preventing aging symptoms of skin, cocoa extract containing polyphenols as cosmetics, more specifically cocoa extract containing a combination of polyphenols, amino acids, and concentrates of substances that cannot be saponified (see Patent Document 4) ), But the cell growth-promoting effect is not shown for the lipid fraction, which is a low-polarity solvent extract of cocoa bean hulls. In addition, there is a report that the non-saponifiable lipid fraction derived from the above cocoa shell partially protected the growth of fibroblasts in a culture solution to which the toxic substance dimethylformamide was added. No report has been made that the growth was promoted by the method (see Non-Patent Document 1).
カカオマスおよびココアパウダーを有効成分とする創傷治癒促進剤（特許文献５、６参照）が開示されているがカカオ豆外皮の中性脂質画分に関する効果は開示されていない。 Although wound healing promoters containing cocoa mass and cocoa powder as active ingredients (see Patent Documents 5 and 6) are disclosed, effects on the neutral lipid fraction of cocoa bean hulls are not disclosed.
また表皮線維芽細胞の増殖促進剤、創傷治癒促進剤として、例えば、サフランの雌しべ抽出物（特許文献７参照）、アーモンド、セイヨウタンポポ、セイヨウニワトコ、センキュウ、センブリ、ソウハクヒ、トウニン、ニンジン、ホップ、ムクゲ、ヨクイニン抽出物（特許文献８参照）などが確認されている。しかしながら、これらの効果は必ずしも満足のいくものではなかった。 Examples of epidermal fibroblast proliferation promoters and wound healing promoters include, for example, saffron pistil extract (see Patent Document 7), almond, dandelion, elderberry, senkyu, assembly, sakuhakuhi, tonin, carrots, hops, muguge Yokuinin extract (see Patent Document 8) and the like have been confirmed. However, these effects were not always satisfactory.
本発明の目的は、カカオ豆外皮抽出物を有効成分とするより有効な皮膚の細胞増殖促進剤およびその製造方法を見出し、かかる細胞増殖促進剤により皮膚の表皮角化細胞および真皮線維芽細胞の増殖を促進させることにより皮膚の老化および肌荒れ症状の防止、改善さらには創傷治癒効果を有する皮膚外用剤、並びに組成物、飲食品を提供することである。 An object of the present invention is to find a more effective skin cell growth promoter and a method for producing the same, comprising a cacao bean hull extract as an active ingredient, and by using the cell growth promoter, skin epidermal keratinocytes and dermal fibroblasts An object of the present invention is to provide a skin external preparation, composition and food / beverage product that have the effect of preventing and improving skin aging and rough skin by promoting proliferation, and further improving wound healing.
本発明者らは、上記課題を解決するため探索を行い、アオギリ科植物のテオブロマ・カカオ（Ｔｈｅｏｂｒｏｍａ ｃａｃａｏ）の外皮抽出物およびその中性脂質画分がそれぞれ高いヒト表皮角化細胞増殖促進作用およびヒト真皮線維芽細胞増殖促進作用を有することを見出し、本発明を完成させた。 In order to solve the above-mentioned problems, the present inventors have conducted a search to promote the proliferation of human epidermis keratinocytes and the epidermis extract of Theobromaceae cabbage (Theobroma cacao) and its high neutral lipid fraction, respectively. The present invention was completed by finding that it has a human dermal fibroblast proliferation promoting action.
すなわち、本発明はカカオ豆外皮抽出物およびその中性脂質画分を有効成分とする表皮角化細胞及び、真皮線維芽細胞の増殖を促進させる皮膚細胞増殖促進剤に関するものである。 That is, the present invention relates to an epidermal keratinocyte containing a cacao bean hull extract and its neutral lipid fraction as active ingredients, and a skin cell proliferation promoter that promotes the proliferation of dermal fibroblasts.
また、本発明はカカオ豆外皮を水と相溶性のない非極性溶媒により抽出し、中性脂質画分を得ることを特徴とする皮膚細胞増殖促進剤の製造方法に関するものである。 The present invention also relates to a method for producing a skin cell growth promoter, characterized in that cocoa bean hulls are extracted with a nonpolar solvent that is incompatible with water to obtain a neutral lipid fraction.
本発明のカカオ豆外皮抽出物およびその中性脂質画分は、表皮角化細胞増殖促進作用および真皮線維芽細胞増殖促進作用を有する。従って、これらを皮膚に適用することにより皮膚の老化を防ぎシワやくすみ、しみを低減させ、弾力の低下の防止及び改善することができ、さらには創傷治癒の促進効果や皮膚再生移植で使用する自家培養表皮細胞及び研究用の皮膚細胞の増殖促進剤としても期待される。 The cocoa bean hull extract of the present invention and its neutral lipid fraction have an action to promote epidermal keratinocyte proliferation and dermal fibroblast proliferation. Therefore, these can be applied to the skin to prevent aging of the skin, reduce wrinkles, dullness and blotches, prevent and improve the decrease in elasticity, and further use for promoting wound healing and skin regeneration transplantation. It is also expected as a growth promoter for autologous cultured epidermal cells and research skin cells.
また、本発明でのカカオ外皮抽出物は、皮膚細胞増殖組成物、皮膚外用剤、飲食品に配合するのに好適なものである。 Moreover, the cacao husk extract in the present invention is suitable for blending into a skin cell growth composition, an external preparation for skin, and food and drink.
以下、本発明を詳細に説明する。本発明の表皮角化細胞増殖促進作用および真皮線維芽細胞増殖促進作用を有する皮膚細胞増殖促進剤それを有効成分とする組成物、皮膚外用剤および飲食品、それらの製造方法、並びにそれらの効果について述べるが、本発明はこれらによって制限されるものではない。 Hereinafter, the present invention will be described in detail. Skin cell growth promoting agent having epidermal keratinocyte proliferation promoting action and dermal fibroblast proliferation promoting action composition of the present invention, composition containing the same as an active ingredient, external preparation for skin and food and drink, production method thereof, and effects thereof However, the present invention is not limited thereto.
本発明で用いるカカオ豆外皮溶媒抽出物及び中性脂質画分を得るカカオ豆外皮は、チョコレート、ココアの原料となるアオギリ科の常緑高木であるカカオの種子から得られ、チョコレート製造時にカカオ豆より剥離されるものであり、剥離方法、カカオ豆の種類等に限定されるものではない。 The cacao bean hull solvent extract used in the present invention and the cacao bean hull to obtain a neutral lipid fraction are obtained from cacao seeds, which are evergreen trees in the family Aogiri, which is a raw material for chocolate and cocoa. It is to be peeled off, and is not limited to the peeling method, the type of cocoa beans, and the like.
脂質は中性脂質と複合脂質（糖脂質、リン脂質）に区別され、中性脂質は誘導脂質及び単純脂質よりなっている。誘導脂質には脂質の加水分解によって誘導される長鎖炭化水素、長鎖アルコール、脂肪酸、テルペノイド、ステロイド等が含まれる。一方、単純脂質は原則として脂肪酸とアルコールが結合したエステル型脂質のことをいい、ワックス、アシルグリセロール、エーテルグリセリド等が含まれる。分画の際、中性脂質は極性がきわめて低く、極性を有する複合脂質とは別の行動を示す。よって、カカオ豆外皮から溶媒抽出物を得るには、特に限定しないが、水と相溶性の無い非極性溶媒、例えば、ヘキサン、エーテル、酢酸エチル、クロロホルム等の有機溶剤もしくはこれらの混合液を抽出溶媒として用いることができる。抽出にあたり、カカオ豆外皮に対して一般的に２〜５０倍量（容量）の抽出溶媒を使用し、好ましくは３〜１０倍量の抽出溶媒を使用する。抽出方法としては、撹拌抽出、還流抽出、浸漬抽出、振とう抽出、超音波抽出などを採用することができ、抽出温度は特に限定しないが２０℃〜１００℃が望ましい。抽出時間は溶媒の種類や抽出温度によって異なるが、３０分〜２日間程度とするのが好ましい。なお、抽出操作は一回に限定されず、抽出効率を上げるため抽出残渣に新鮮な溶媒を再度添加し、抽出操作を繰り返してもよい。溶媒抽出物は、濾過または遠心分離等により、溶媒抽出物と植物とを分離するのが良い。更に得られた抽出物は、適当な濃縮処理により、例えばエバポレーターのような減圧濃縮装置や加熱による溶媒除去などにより濃縮し濃縮液を得る事が出来る。さらに濃縮液を乾燥させて濃縮乾固物を得ることも出来る。 Lipids are classified into neutral lipids and complex lipids (glycolipids, phospholipids), and neutral lipids are composed of derived lipids and simple lipids. Derived lipids include long chain hydrocarbons, long chain alcohols, fatty acids, terpenoids, steroids, and the like that are derived from lipid hydrolysis. On the other hand, simple lipids are in principle ester-type lipids in which fatty acids and alcohols are combined, and include waxes, acylglycerols, ether glycerides and the like. During fractionation, neutral lipids have very low polarity and behave differently from complex lipids with polarity. Therefore, to obtain a solvent extract from cocoa bean hulls, although not particularly limited, extract a nonpolar solvent that is not compatible with water, for example, an organic solvent such as hexane, ether, ethyl acetate, chloroform, or a mixture thereof. It can be used as a solvent. In the extraction, the extraction solvent is generally used in an amount of 2 to 50 times (volume) with respect to the cocoa bean hull, preferably 3 to 10 times the amount of the extraction solvent. As the extraction method, stirring extraction, reflux extraction, immersion extraction, shaking extraction, ultrasonic extraction and the like can be adopted, and the extraction temperature is not particularly limited, but 20 ° C. to 100 ° C. is desirable. The extraction time varies depending on the type of solvent and the extraction temperature, but is preferably about 30 minutes to 2 days. In addition, extraction operation is not limited to once, In order to raise extraction efficiency, a fresh solvent may be added again to an extraction residue, and extraction operation may be repeated. The solvent extract is preferably separated from the plant and the plant by filtration or centrifugation. Further, the obtained extract can be concentrated by an appropriate concentration treatment, for example, by a vacuum concentration device such as an evaporator, or by removing the solvent by heating, to obtain a concentrated solution. Further, the concentrated solution can be dried to obtain a concentrated dried product.
カカオ豆外皮の中性脂質画分は、カカオ豆外皮抽出物を液−液分配、カラムクロマトグラフィー、薄層クロマト、高速液体クロマトグラフィーなどの方法により得る事ができる。特に限定しないが、液−液分配による中性脂質画分の調製例として、カカオ豆外皮抽出物をＧａｌａｎｏｓ＆Ｋａｐｏｕｌａｓの石油エーテルエタノール分配法を用いて中性脂質画分と複合脂質画分に分配出来る。 The neutral lipid fraction of cocoa bean hull can be obtained by cocoa bean hull extract by methods such as liquid-liquid partition, column chromatography, thin layer chromatography, high performance liquid chromatography and the like. Although it does not specifically limit, As an example of preparation of the neutral lipid fraction by liquid-liquid distribution, a cacao bean hull extract can be distributed into a neutral lipid fraction and a complex lipid fraction using the petroleum ether ethanol distribution method of Galanos & Kapoulas.
カラムクロマトグラフィーによる中性脂質画分の調製例としてカカオ豆外皮抽出物をヘキサン又はクロロホルム等に溶解後、シリカゲルクロマトグラフィーに注入した。ヘキサン又はクロロホルムを流下し、溶出液を回収することにより、精製された中性脂質画分を得た。又は定法に従い、ＤＥＡＥ−セルロースカラムグラフィーを用いても中性脂質画分を得る事ができる。 As an example of preparing a neutral lipid fraction by column chromatography, a cacao bean hull extract was dissolved in hexane or chloroform and then injected into silica gel chromatography. Purified neutral lipid fraction was obtained by flowing hexane or chloroform down and collecting the eluate. Or according to a usual method, a neutral lipid fraction can also be obtained using DEAE-cellulose columnography.
薄層クロマトによる中性脂質画分の調製例として、薄層プレート（Ｓｌｉｃａ Ｇｅｌ ６０）上にクロロホルムに溶解させたカカオ豆外皮抽出物をスポットし、クロロホルム／メタノール／水＝８０：２５：４の溶液で展開させる。発色処理後、中性脂質を含むスポットをかき取り、クロロホルム溶液中へ入れ十分に撹拌後、ガラスフィルターにて濾過し、得られた濾液を濃縮することにより中性脂質画分を得る事ができる。 As an example of preparing a neutral lipid fraction by thin layer chromatography, a cocoa bean shell extract dissolved in chloroform was spotted on a thin layer plate (Slica Gel 60), and chloroform / methanol / water = 80: 25: 4. Develop with solution. After the color development treatment, the neutral lipid fraction can be obtained by scraping off the spot containing neutral lipid, placing it in a chloroform solution, stirring sufficiently, filtering through a glass filter, and concentrating the resulting filtrate. .
本発明の増殖促進剤はそのままでも使用出来るが、濃縮、または乾固させて使用してもよい。また、増殖促進剤は、必要に応じて上記抽出物に、保存やその取り扱いを容易にするため、ショ糖や乳糖などの各種糖類、たんぱく質やデンプンなどの賦形剤、その他の任意の助剤を用いてもよい。また、例えば、粉末状、顆粒状、錠剤状などの任意の剤形として製剤化して提供することも可能である。 The growth promoter of the present invention can be used as it is, but may be used after being concentrated or dried. In addition, in order to facilitate the storage and handling of the above-mentioned extract as necessary, the growth promoting agent is various sugars such as sucrose and lactose, excipients such as protein and starch, and other optional auxiliary agents. May be used. In addition, for example, it can be formulated and provided as an arbitrary dosage form such as powder, granule, tablet and the like.
本発明の皮膚外用剤には、本発明の皮膚細胞増殖促進剤の成分以外に、通常化粧品や医薬品等の皮膚外用剤に用いられる成分、例えば油類、界面活性剤、保湿剤、紫外線吸収剤、色材、酸化防止剤、増粘剤、アルコール類、粉末成分、水性成分、水、各種皮膚栄養剤、各種薬剤、キレート剤、ｐＨ調製剤等や、活性酸素消去剤、抗炎症剤、美白剤等の生理活性成分を必要に応じて適宜配合することができる。また、本発明の皮膚外用剤とは、通常医薬品、医薬部外品、化粧品等の分野で用いられるものを指し、その剤型は本発明の効果が発揮できる限り、特に限定されるものではない。例えば軟膏、クリーム、乳液、ローション、パック、浴用剤等、従来皮膚外用剤に用いられているものであればいずれでもよい。 In addition to the components of the skin cell growth promoter of the present invention, the topical skin preparation of the present invention includes components usually used for skin external preparations such as cosmetics and pharmaceuticals, such as oils, surfactants, moisturizers, ultraviolet absorbers. , Coloring materials, antioxidants, thickeners, alcohols, powder components, aqueous components, water, various skin nutrients, various drugs, chelating agents, pH adjusting agents, active oxygen scavengers, anti-inflammatory agents, whitening A physiologically active ingredient such as an agent can be appropriately blended as necessary. The topical skin preparation of the present invention refers to those usually used in the fields of pharmaceuticals, quasi drugs, cosmetics, etc. The dosage form is not particularly limited as long as the effect of the present invention can be exhibited. . For example, any ointment, cream, milky lotion, lotion, pack, bath preparation and the like conventionally used for external preparations for skin may be used.
又、表皮及び真皮の増殖促進、老化防止、肌荒れ並びに創傷治癒促進などを目的として、皮膚以外の頭皮、口腔、耳、鼻、肛門、陰部などの様々な外傷にも用いることができる。 It can also be used for various injuries such as scalp other than skin, oral cavity, ear, nose, anus, and genital area for the purpose of promoting proliferation of epidermis and dermis, preventing aging, rough skin, and promoting wound healing.
さらに、本発明の皮膚細胞増殖促進剤は、各種飲食素材として使用することができる。例えばチューインガム、キャンディ、錠菓、グミゼリー、チョコレート、ビスケット、アイスクリーム、飲料等に配合し、日常的に利用することが可能である。 Furthermore, the skin cell proliferation promoter of the present invention can be used as various food and drink materials. For example, it can be blended into chewing gum, candy, tablet confectionery, gummy jelly, chocolate, biscuits, ice cream, beverages, etc. and used on a daily basis.
本発明に係る抽出物の皮膚外用剤、飲食品中への配合量は、その目的、剤形により異なるが、０．０１〜８０重量％程度とするのが適当である。 The blending amount of the extract according to the present invention into the external preparation for skin and food and drink varies depending on the purpose and dosage form, but is suitably about 0.01 to 80% by weight.
以下、本発明の実施例を説明するが、本発明は、これらの実施例に何ら限定されるものではない。 Examples of the present invention will be described below, but the present invention is not limited to these examples.
−カカオ豆外皮抽出物の調製−
（製造例１）
カカオ豆外皮に含まれる中性脂質、糖脂質、リン脂質を回収する抽出法としてＦｏｌｃｈ法に準じて行った。カカオ豆外皮（１００ｇ）はミルで粉砕後、カカオ豆外皮の１重量部にクロロホルム／メタノール（２：１）溶液を５重量部加え、室温下で３時間振とう後、濾過により抽出液を回収した。更に残渣を同様な方法で３回繰り返し抽出した。得られた濾液に対し２０％容量の水を添加撹拌後、分液ロートにて下層のクロロホルム層を回収し乾固させた。回収物はエタノール／水（８７：１３）と石油エーテルの等量混合液に溶解させ、分液ロートで分離させた上層と下層のそれぞれを回収し、乾固させた。上層の石油エーテル層からの回収物はクロロホルムに溶かし、シリカゲルクロマトグラフィーに注入し、クロロホルム、アセトン、メタノールを連続的に流下させ、各溶出液を回収した。下層からの回収物もクロロホルムに溶解しシリカゲルクロマトグラフィーに注入し、クロロホルム、アセトン、メタノールを連続的に流下させ、各溶出液を回収した。上記の溶出液は溶媒ごとに回収し乾固させた。クロロホルム溶出液から中性脂質画分（５．９ｇ）、アセトン溶出液から糖脂質画分（０．７５ｇ）、メタノール溶出液からリン脂質画分（０．１４ｇ）を得た。
-Preparation of cocoa bean hull extract-
(Production Example 1)
The extraction method for recovering neutral lipids, glycolipids and phospholipids contained in cocoa bean hulls was performed according to the Folch method. Cacao bean hulls (100 g) are pulverized in a mill, 1 part by weight of cocoa bean hulls is added with 5 parts by weight of a chloroform / methanol (2: 1) solution, shaken at room temperature for 3 hours, and the extract is recovered by filtration. did. Further, the residue was extracted three times in the same manner. A 20% volume of water was added to the obtained filtrate and stirred, and then the lower chloroform layer was collected with a separatory funnel and dried. The recovered product was dissolved in an equal volume mixture of ethanol / water (87:13) and petroleum ether, and each of the upper layer and the lower layer separated by a separatory funnel was recovered and dried. The recovered material from the upper petroleum ether layer was dissolved in chloroform and injected into silica gel chromatography, and chloroform, acetone and methanol were continuously allowed to flow down to recover each eluate. The recovered material from the lower layer was also dissolved in chloroform and injected into silica gel chromatography, and chloroform, acetone, and methanol were allowed to flow continuously to recover each eluate. The eluate was collected for each solvent and dried. A neutral lipid fraction (5.9 g) was obtained from the chloroform eluate, a glycolipid fraction (0.75 g) was obtained from the acetone eluate, and a phospholipid fraction (0.14 g) was obtained from the methanol eluate.
（製造例２）
カカオ豆外皮（１００ｇ）はミルで粉砕後、カカオ豆外皮の１重量部に酢酸エチルを５重量部加え、室温下で３時間振とう後、濾過により抽出液を回収した。更に残渣を同様の方法で３回繰り返し抽出した。得られた濾液はロータリーエバポレーターで濃縮し少量のクロロホルムに溶解後、シリカゲルクロマトグラフィーに注入した。クロロホルムを流下することによって中性脂質画分（５．９８ｇ）を得た。
(Production Example 2)
The cocoa bean hull (100 g) was pulverized by a mill, 5 parts by weight of ethyl acetate was added to 1 part by weight of the cocoa bean hull, shaken at room temperature for 3 hours, and the extract was collected by filtration. Further, the residue was extracted repeatedly three times in the same manner. The obtained filtrate was concentrated by a rotary evaporator, dissolved in a small amount of chloroform, and injected into silica gel chromatography. A neutral lipid fraction (5.98 g) was obtained by flowing down chloroform.
（製造例３）
カカオ豆外皮（１００ｇ）はミルで粉砕後、カカオ豆外皮の１重量部にヘキサンを５重量部加え、室温下で２４時間振とう後、濾過により抽出液を回収した。更に残渣を同様な方法で３回繰り返し抽出し、中性脂質画分（６．０３ｇ）を得た。
(Production Example 3)
The cocoa bean hull (100 g) was pulverized with a mill, 5 parts by weight of hexane was added to 1 part by weight of the cocoa bean hull, shaken at room temperature for 24 hours, and the extract was collected by filtration. Further, the residue was repeatedly extracted by the same method three times to obtain a neutral lipid fraction (6.03 g).
−カカオニブ抽出物の調製−
（比較例１）
カカオニブに含まれる中性脂質、糖脂質、リン脂質を回収する抽出法として、Ｆｏｌｃｈ法に準じて抽出を行った。カカオ豆からカカオ豆外皮を除去したカカオニブ（１００ｇ）を乳棒と乳鉢にてすりつぶし、カカオニブの１重量部にクロロホルム／メタノール（２：１）溶液を５重量部加え、室温下で３時間振とう後、濾過により抽出液を回収した。更に残渣を同様な方法で３回繰り返し抽出した。得られた濾液に対し２０％容量の水を添加撹拌後、分液ロートにて下層のクロロホルム層を回収し乾固させた。回収物はエタノール／水（８７：１３）と石油エーテルの等量混合液に溶解させ、分液ロートで分離させた上層と下層のそれぞれを回収し、乾固させた。上層の石油エーテル層からの回収物はクロロホルムに溶かし、シリカゲルクロマトグラフィーに注入し、クロロホルム、アセトン、メタノールを連続的に流下させ、各溶出液を回収した。下層からの回収物もクロロホルムに溶解しシリカゲルクロマトグラフィーに注入し、クロロホルム、アセトン、メタノールを連続的に流下させ、各溶出液を回収した。上記の溶出液は溶媒ごとに回収し乾固させた。クロロホルム溶出液から中性脂質画分（５８．９ｇ）、アセトン溶出液から糖脂質画分（０．６ｇ）、メタノール溶出液からリン脂質画分（０．６ｇ）を得た。
-Preparation of cacao nib extract-
(Comparative Example 1)
As an extraction method for recovering neutral lipids, glycolipids, and phospholipids contained in cacao nibs, extraction was performed according to the Folch method. Cacao nibs (100 g) from which cacao bean hulls have been removed from cocoa beans are ground with a pestle and mortar, 5 parts by weight of a chloroform / methanol (2: 1) solution is added to 1 part by weight of the cocoa nibs, and shaken at room temperature for 3 hours. The extract was recovered by filtration. Further, the residue was extracted three times in the same manner. A 20% volume of water was added to the obtained filtrate and stirred, and then the lower chloroform layer was collected with a separatory funnel and dried. The recovered product was dissolved in an equal volume mixture of ethanol / water (87:13) and petroleum ether, and each of the upper layer and the lower layer separated by a separatory funnel was recovered and dried. The recovered material from the upper petroleum ether layer was dissolved in chloroform and injected into silica gel chromatography, and chloroform, acetone and methanol were continuously allowed to flow down to recover each eluate. The recovered material from the lower layer was also dissolved in chloroform and injected into silica gel chromatography, and chloroform, acetone, and methanol were allowed to flow continuously to recover each eluate. The eluate was collected for each solvent and dried. A neutral lipid fraction (58.9 g) was obtained from the chloroform eluate, a glycolipid fraction (0.6 g) was obtained from the acetone eluate, and a phospholipid fraction (0.6 g) was obtained from the methanol eluate.
（試験例１）
表皮角化細胞増殖促進作用試験
試験試料として製造例１、比較例１で得られたカカオニブとカカオ豆外皮の中性脂質画分、糖脂質画分、リン脂質画分について、表皮細胞増殖の評価を行った。細胞増殖評価法は、正常ヒト表皮角化細胞を１ウェル当たり１．０×１０４ 個となるように９６穴マイクロプレートに播種し、カカオニブとハスク脂質の各抽出画分を２０μｇ／ｍｌ〜１００μｇ／ｍｌの各試験濃度含有する表皮角化細胞増殖用培地（Ｈｕｍｅｄｉａ−ＫＧ２）にて３７℃で２４時間培養し、次いで２−（４−Ｉｏｄｏｐｈｅｎｙｌ）−３−（４−ｎｉｔｒｏｐｈｅｎｙｌ）−５−（２，４−ｄｉｓｕｌｆｏｐｈｅｎｙｌ）−２Ｈ−ｔｅｔｒａｚｏｌｉｕｍ，ｍｏｎｏｓｏｄｉｕｍ ｓａｌｔ（ＷＳＴ−１）を１ウェル当たり１０μｌ添加し、３７℃で３時間培養し、テトラゾリウム環の開環により生じるフォルマザンをマイクロプレートリーダーにてリファレンス波長を６５５ｎｍとし、４５０ｎｍの吸光度（Ａｓ）を測定した。同様にして試験試料を添加せずに正常ヒト表皮角化細胞を培養し、上記と同様の方法でマイクロプレートリーダーにて測定した値をＡｂとし、細胞増殖率をＡｓ／Ａｂ×１００（％）で計算し表１に示した。
(Test Example 1)
Epidermal keratinocyte growth-promoting action test Evaluation of epidermal cell proliferation for the neutral lipid fraction, glycolipid fraction, and phospholipid fraction of cacao nibs and cacao bean hulls obtained in Production Example 1 and Comparative Example 1 as test samples Went. In the cell proliferation evaluation method, normal human epidermal keratinocytes are seeded in a 96-well microplate so as to be 1.0 × 10 4 cells per well, and each extracted fraction of cacao nibs and husk lipids is 20 μg / ml to 100 μg. Culturing for 24 hours at 37 ° C. in an epidermal keratinocyte growth medium (Humdia-KG2) containing each test concentration of / ml, then 2- (4-Iodophenyl) -3- (4-nitrophenyl) -5- ( 2,4-disulphophenyl) -2H-tetrazolium, monosodium salt (WST-1) was added at 10 μl per well, cultured at 37 ° C. for 3 hours, and formazan generated by the opening of the tetrazolium ring was referred to the reference wavelength with a microplate reader. Was measured at 450 nm and the absorbance (As) at 450 nm was measured. It was. Similarly, normal human epidermal keratinocytes were cultured without adding a test sample, and the value measured with a microplate reader in the same manner as described above was defined as Ab, and the cell growth rate was As / Ab × 100 (%). And are shown in Table 1.
表１の結果から、製造例１のカカオ外皮の中性脂質画分は１００μｇ／ｍｌ濃度で有意に表皮角化細胞の増殖を促進させていた。またカカオ外皮の糖脂質も細胞の増殖促進傾向が確認された。一方、比較例１のカカオニブ由来の脂質画分には活性が認められなかった。 From the results in Table 1, the neutral lipid fraction of the cocoa shell of Production Example 1 significantly promoted the growth of epidermal keratinocytes at a concentration of 100 μg / ml. In addition, the glycolipids of cacao husks were confirmed to have a tendency to promote cell growth. On the other hand, no activity was observed in the lipid fraction derived from cacao nibs of Comparative Example 1.
（試験例２）
表皮角化細胞増殖促進作用試験
製造例２、３で得られたカカオ豆外皮の酢酸エチル抽出物、ヘキサン抽出物について、表皮細胞増殖作用の評価を行った。評価法は、試験例１と同様の方法にて行い、表２に結果を示した。その結果、製造例２、３の酢酸エチル抽出物及びヘキサン抽出物は１００μｇ／ｍｌ濃度で表皮角化細胞の増殖を促進させていた。
(Test Example 2)
Epidermal keratinocyte growth promoting action test The ethyl acetate extract and hexane extract of cocoa bean hulls obtained in Production Examples 2 and 3 were evaluated for epidermal cell proliferation action. The evaluation method was the same as in Test Example 1, and the results are shown in Table 2. As a result, the ethyl acetate extract and the hexane extract of Production Examples 2 and 3 promoted the growth of epidermal keratinocytes at a concentration of 100 μg / ml.
（試験例３）
真皮線維芽細胞増殖促進作用試験
試験試料として製造例１、比較例１で得られたカカオニブとカカオ豆外皮のそれぞれについての中性脂質画分、糖脂質画分、リン脂質画分について、真皮細胞増殖作用の評価を行った。細胞増殖評価法は、正常ヒト真皮線維芽細胞を１ウェル当たり０．３２×１０４ 個となるように９６穴マイクロプレートに播種し、カカオニブとハスク脂質の各抽出画分を２０μｇ／ｍｌ〜１００μｇ／ｍｌの各試験濃度含有する真皮繊維芽細胞増殖用培地（Ｍｅｄｉｕｍ１０６Ｓ）にて３７℃で２４時間培養し、次いで２−（４−Ｉｏｄｏｐｈｅｎｙｌ）−３−（４−ｎｉｔｒｏｐｈｅｎｙｌ）−５−（２，４−ｄｉｓｕｌｆｏｐｈｅｎｙｌ）−２Ｈ−ｔｅｔｒａｚｏｌｉｕｍ，ｍｏｎｏｓｏｄｉｕｍ ｓａｌｔ（ＷＳＴ−１）を１ウェル当たり１０μｌ添加し、３７℃で３時間培養し、テトラゾリウム環の開環により生じるフォルマザンを１ウェル当たり１０μｌ添加し、３７℃で３時間培養し、テトラゾリウム環の開環により生じるフォルマザンをマイクロプレートリーダーにてリファレンス波長を６５５ｎｍとし、４５０ｎｍの吸光度（Ａｓ）を測定した。同様にして試験試料を添加せずに正常ヒト真皮線維芽細胞を培養し、上記と同様の方法でマイクロプレートリーダーにて測定した値をＡｂとし、細胞増殖率をＡｓ／Ａｂ×１００（％）で計算し表３に示した。
(Test Example 3)
Test for promoting dermal fibroblast proliferation About the neutral lipid fraction, glycolipid fraction, and phospholipid fraction of cocoa nibs and cocoa bean hulls obtained in Production Example 1 and Comparative Example 1 as test samples, dermal cells Proliferative effects were evaluated. In the cell proliferation evaluation method, normal human dermal fibroblasts are seeded in a 96-well microplate so that the number of normal human dermal fibroblasts is 0.32 × 10 4 per well, and each extracted fraction of cacao nibs and husk lipids is 20 μg / ml to 100 μg. Culturing for 24 hours at 37 ° C. in dermal fibroblast growth medium (Medium 106S) containing each test concentration of / ml, then 2- (4-Iodophenyl) -3- (4-nitrophenyl) -5- (2, 4-disulphophenyl) -2H-tetrazolium, monosodium salt (WST-1) was added at 10 μl per well, cultured at 37 ° C. for 3 hours, and formazan formed by opening of the tetrazolium ring was added at 10 μl per well. Incubate for 3 hours in Than the 655nm reference wavelength at microplate reader, measured 450nm absorbance of (As). Similarly, normal human dermal fibroblasts were cultured without adding a test sample, and the value measured with a microplate reader in the same manner as described above was defined as Ab, and the cell proliferation rate was As / Ab × 100 (%). And are shown in Table 3.
表３の結果から、製造例１のカカオ外皮の中性脂質画分は６０μｇ／ｍｌ濃度で有意に真皮線維芽細胞の増殖を促進させていることが確認できた。一方、比較例１のカカオニブ由来の脂質画分には活性が認められなかった。 From the results in Table 3, it was confirmed that the neutral lipid fraction of cocoa shell of Production Example 1 significantly promoted the proliferation of dermal fibroblasts at a concentration of 60 μg / ml. On the other hand, no activity was observed in the lipid fraction derived from cacao nibs of Comparative Example 1.
（試験例４）
表皮角化細胞増殖促進作用試験
製造例２、３で得られたカカオ豆外皮のヘキサン抽出画分（中性脂質画分）について、真皮細胞増殖作用の評価を行った。評価法は、試験例１と同様の方法にて行い、表４に結果を示した。その結果、製造例２、３の酢酸エチル抽出物及びヘキサン抽出物は１００μｇ／ｍｌ濃度で真皮線維芽細胞の増殖を促進させていた。
(Test Example 4)
Epidermal keratinocyte proliferation promoting action test The dermal cell proliferation action of the hexane-extracted fraction (neutral lipid fraction) of the cocoa bean hull obtained in Production Examples 2 and 3 was evaluated. The evaluation method was the same as in Test Example 1, and the results are shown in Table 4. As a result, the ethyl acetate extract and hexane extract of Production Examples 2 and 3 promoted the growth of dermal fibroblasts at a concentration of 100 μg / ml.
以下、実施例を挙げて本発明を更に詳細に説明するが、各実施例は各製品の製造における常法により製造したもので良く、配合量のみを示した。それらによって本発明品の範囲を制限するものではない。また、各実施例における製品の配合の際に使用するカカオ豆外皮中性脂質画分は、上記製造例１、２および３で製造された中性脂質画分のいずれを利用しても良い。 EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated still in detail, each Example may be manufactured by the conventional method in manufacture of each product, and showed only the compounding quantity. They do not limit the scope of the present invention. Moreover, any of the neutral lipid fractions produced in Production Examples 1, 2, and 3 may be used as the cocoa bean husk neutral lipid fraction used in blending the products in each Example.
（実施例１） 錠剤の処方
Ｄ−マンニトール ４２．６重量％
乳糖 ４２．６
結晶セルロース ８．５
ヒドロキシプロピルセルロース ４．３
カカオ豆外皮中性脂質画分（製造例１） ２．０
１００．０
Example 1 Tablet formulation D-mannitol 42.6% by weight
Lactose 42.6
Crystalline cellulose 8.5
Hydroxypropylcellulose 4.3
Cocoa bean shell neutral lipid fraction (Production Example 1) 2.0
100.0
（実施例２） 軟膏剤の処方
ミツロウ ３．０重量％
水素添加ラノリン ８．０
スクワラン ３４．０
固形パラフィン ２．０
マイクロクリスタリンワックス ９．０
白色ワセリン ５．０
アジピン酸ヘキシルデシル １３．０
セスキオレイン酸ソルビタン ３．５
ポリオキシエチレン硬化ヒマシ油 １．０
グリセリン １０．０
エタノール １．０
カカオ豆外皮中性脂質画分（製造例１） １．０
水 残
１００．０
(Example 2) Beeswax formulation 3.0% by weight
Hydrogenated lanolin 8.0
Squalane 34.0
Solid paraffin 2.0
Microcrystalline wax 9.0
White petrolatum 5.0
Hexyldecyl adipate 13.0
Sorbitan sesquioleate 3.5
Polyoxyethylene hydrogenated castor oil 1.0
Glycerin 10.0
Ethanol 1.0
Cocoa bean shell neutral lipid fraction (Production Example 1) 1.0
Water remaining
100.0
（実施例３） クリームの処方
スクワラン ２７．０重量％
ミツロウ ６．０
セタノール ５．０
還元ラノリン ８．０
グリセリン脂肪酸エステル ４．０
親油型グリセリルモノスレアリン酸エステル ２．０
ポリオキシエチレンソルビタンモノラウリン酸エステル ５．０
プロピレングリコール ５．０
パラオキシ安息香酸メチル ０．１
カカオ豆外皮中性脂質画分（製造例２） １．０
水 残
１００．０
Example 3 Cream Formulation Squalane 27.0 wt%
Beeslow 6.0
Cetanol 5.0
Reduced lanolin 8.0
Glycerin fatty acid ester 4.0
Lipophilic glyceryl monosreaphosphate ester 2.0
Polyoxyethylene sorbitan monolaurate 5.0
Propylene glycol 5.0
Methyl paraoxybenzoate 0.1
Cocoa bean shell neutral lipid fraction (Production Example 2) 1.0
Water remaining
100.0
（実施例４） 乳液の処方
スクワラン １０．０重量％
メチルフェニルポリシロキサン ４．０
水素添加パーム核油 ０．５
水素添加大豆リン脂質 ０．１
モノステアリン酸ポリオキシエチレンソルビタン １．３
モノステアリン酸ソルビタン １．０
グリセリン ４．０
パラオキシ安息香酸メチル ０．１
カルボキシビニルポリマー ０．１５
カカオ豆外皮中性脂質画分（製造例３） ５．０
香料 適量
水 残
１００．０
(Example 4) Prescription squalane 10.0% by weight of emulsion
Methylphenyl polysiloxane 4.0
Hydrogenated palm kernel oil 0.5
Hydrogenated soybean phospholipid 0.1
Polyoxyethylene sorbitan monostearate 1.3
Sorbitan monostearate 1.0
Glycerin 4.0
Methyl paraoxybenzoate 0.1
Carboxyvinyl polymer 0.15
Cocoa bean shell neutral lipid fraction (Production Example 3) 5.0
Perfume
Water remaining
100.0
（実施例５） ローションの処方
ジプロピレングリコール ５．０重量％
１，３−ブチレングリコール １０．０
ポリエチレングリコール４００ １０．０
ポリオキシエチレン硬化ヒマシ油 ３．０
エタノール ２０．０
パラメトキシケイ皮酸オクチル １．０
アルブチン ４．０
亜硫酸水素ナトリウム ０．０３
アスコルビン酸グルコシド ５．０
トリエタノールアミン ５．０
香料 ０．１
カカオ外豆皮中性脂質画分（製造例１） ５．０
水 残
１００．０
(Example 5) Formulation of lotion Dipropylene glycol 5.0 wt%
1,3-butylene glycol 10.0
Polyethylene glycol 400 10.0
Polyoxyethylene hydrogenated castor oil 3.0
Ethanol 20.0
Octyl paramethoxycinnamate 1.0
Arbutin 4.0
Sodium bisulfite 0.03
Ascorbic acid glucoside 5.0
Triethanolamine 5.0
Fragrance 0.1
Cocoa outer soybean hull neutral lipid fraction (Production Example 1) 5.0
Water remaining
100.0
（実施例６） 美容液の処方
グリセリン １０．０重量％
ショ糖脂肪酸エステル １．３
カルボキシビニルポリマー（１重量％水溶液） １７．５
アルギン酸ナトリウム（１重量％水溶液） １５．０
モノラウリン酸ポリグリセリル １．０
マカデミアナッツ油脂肪酸フィトステリル ３．０
Ｎ−ラウロイル−Ｌ−グルタミン酸
ジ（フィトステリル−２−オクチルドデシル） ２．０
硬化パーム油 ２．０
スクワラン １．０
ベヘニルアルコール ０．７５
ミツロウ １．０
ホホバ油 １．０
１，３−ブチレングリコール １０．０
Ｌ−アルギニン（１０重量％水溶液） ２．０
カカオ豆外皮中性脂質画分（製造例１） ５．０
水 残
１００．０
(Example 6) Prescription glycerin of cosmetic liquid 10.0% by weight
Sucrose fatty acid ester 1.3
Carboxyvinyl polymer (1 wt% aqueous solution) 17.5
Sodium alginate (1% by weight aqueous solution) 15.0
Polyglyceryl monolaurate 1.0
Macadamia nut oil fatty acid phytosteryl 3.0
N-lauroyl-L-glutamate di (phytosteryl-2-octyldodecyl) 2.0
Hardened palm oil 2.0
Squalane 1.0
Behenyl alcohol 0.75
Beeswax 1.0
Jojoba oil 1.0
1,3-butylene glycol 10.0
L-arginine (10% by weight aqueous solution) 2.0
Cocoa bean shell neutral lipid fraction (Production Example 1) 5.0
Water remaining
100.0
（実施例７） 洗顔料の処方
ステアリン酸 １６．０重量％
ミリスチン酸 １６．０
親油型モノステアリン酸グリセリン ２．０
グリセリン ２０．０
水酸化ナトリウム ７．５
ヤシ油脂肪酸アミドプロピルベタイン １．０
カカオ豆外皮中性脂質画分（製造例２） １．０
水 残
１００．０
(Example 7) Formulation of face wash 16.0% by weight stearic acid
Myristic acid 16.0
Lipophilic glyceryl monostearate 2.0
Glycerin 20.0
Sodium hydroxide 7.5
Coconut oil fatty acid amidopropyl betaine 1.0
Cocoa bean shell neutral lipid fraction (Production Example 2) 1.0
Water remaining
100.0
（実施例８） パックの処方
ポリビニルアルコール １２．０重量％
エタノール １０．０
グリセリン ５．０
ポリエチレングリコール（平均分子量１０００） ２．０
カカオ豆外皮中性脂質画分（製造例１） ５．０
香料 ０．１
水 残
１００．０
Example 8 Pack formulation polyvinyl alcohol 12.0 wt%
Ethanol 10.0
Glycerin 5.0
Polyethylene glycol (average molecular weight 1000) 2.0
Cocoa bean shell neutral lipid fraction (Production Example 1) 5.0
Fragrance 0.1
Water remaining
100.0
（実施例９） 入浴剤の処方
炭酸水素ナトリウム ４９．０重量％
硫酸ナトリウム ４９．７
香料 ０．３
カカオ外皮中性脂質画分（製造例２） １．０
１００．０
(Example 9) Formulation of bathing agent Sodium hydrogen carbonate 49.0% by weight
Sodium sulfate 49.7
Fragrance 0.3
Cocoa shell neutral lipid fraction (Production Example 2) 1.0
100.0
（実施例１０） チューインガムの処方
ガムベース ２０．０重量％
砂糖 ５５．０
グルコース １５．０
水飴 ９．０
香料 ０．５
カカオ豆外皮中性脂質画分（製造例３） ０．５
１００．０
Example 10 Chewing gum formula gum base 20.0% by weight
Sugar 55.0
Glucose 15.0
Minamata 9.0
Fragrance 0.5
Cocoa bean shell neutral lipid fraction (Production Example 3) 0.5
100.0
（実施例１１） キャンディの処方
砂糖 ５０．０重量％
水飴 ３４．０
香料 ０．５
カカオ豆外皮中性脂質画分（製造例３） ０．５
水 残
１００．０
(Example 11) Candy prescription sugar 50.0% by weight
Minamata 34.0
Fragrance 0.5
Cocoa bean shell neutral lipid fraction (Production Example 3) 0.5
Water remaining
100.0
（実施例１２） 錠菓の処方
砂糖 ７６．４重量％
グルコース １９．０
ショ糖脂肪酸エステル ０．２
香料 ０．２
カカオ豆外皮中性脂質画分（製造例３） ０．１
水 残
１００．０
(Example 12) Prescription sugar for tablet candy 76.4% by weight
Glucose 19.0
Sucrose fatty acid ester 0.2
Fragrance 0.2
Cocoa bean shell neutral lipid fraction (Production Example 3) 0.1
Water remaining
100.0
（実施例１３） チョコレートの処方
粉糖 ３９．８重量％
カカオビター ２０．０
全脂粉乳 ２０．０
カカオバター １７．０
マンニトール ２．０
カカオ豆外皮中性脂質画分（製造例３） １．０
香料 ０．２
１００．０
(Example 13) Chocolate powdered sugar 39.8% by weight
Cocoa bitter 20.0
Whole milk powder 20.0
Cocoa butter 17.0
Mannitol 2.0
Cocoa bean shell neutral lipid fraction (Production Example 3) 1.0
Fragrance 0.2
100.0
（実施例１４） ビスケットの処方
薄力粉１級 ２５．５９重量％
中力粉１級 ２２．２２
精白糖 ４．８
食塩 ０．７３
ブドウ糖 ０．７８
パームショートニング １１．７８
炭酸水素ナトリウム ０．１７
重亜硫酸ナトリウム ０．１６
米粉 １．４５
全脂粉乳 １．１６
代用粉乳 ０．２９
カカオ豆外皮中性脂質画分（製造例３） ０．５
水 残
１００．０
(Example 14) Biscuit prescription weak flour grade 1 25.59 wt%
Medium strength powder 1st class 22.22
Refined sugar 4.8
Salt 0.73
Glucose 0.78
Palm shortening 11.78
Sodium bicarbonate 0.17
Sodium bisulfite 0.16
Rice flour 1.45
Whole milk powder 1.16
Substitute milk powder 0.29
Cocoa bean shell neutral lipid fraction (Production Example 3) 0.5
Water remaining
100.0
（実施例１５） アイスクリームの処方
脱脂粉乳 ５０．０重量％
生クリーム ２５．０
砂糖 １０．０
卵黄 １０．０
カカオ豆外皮中性脂質画分（製造例３） ０．１
香料 ０．１
水 残
１００．０
(Example 15) Prescription skim milk powder of ice cream 50.0% by weight
Fresh cream 25.0
Sugar 10.0
Egg yolk 10.0
Cocoa bean shell neutral lipid fraction (Production Example 3) 0.1
Fragrance 0.1
Water remaining
100.0
（実施例１６） 飲料の処方
オレンジ果汁 ３０．０重量％
異性化糖 １５．２４
クエン酸 ０．１
ビタミンＣ ０．０４
香料 ０．１
カカオ豆外皮中性脂質画分（製造例３） ０．１
水 残
１００．０
(Example 16) Prescription orange juice 30.0% by weight of beverage
Isomerized sugar 15.24
Citric acid 0.1
Vitamin C 0.04
Fragrance 0.1
Cocoa bean shell neutral lipid fraction (Production Example 3) 0.1
Water remaining
100.0
Claims (8)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007044766A JP2008208053A (en) | 2007-02-23 | 2007-02-23 | Composition for promoting skin cell growth and method for producing the same |
| KR1020080016028A KR100973782B1 (en) | 2007-02-23 | 2008-02-21 | Composition for promotion of skin cell growth and process for preparation thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007044766A JP2008208053A (en) | 2007-02-23 | 2007-02-23 | Composition for promoting skin cell growth and method for producing the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2008208053A true JP2008208053A (en) | 2008-09-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007044766A Withdrawn JP2008208053A (en) | 2007-02-23 | 2007-02-23 | Composition for promoting skin cell growth and method for producing the same |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP2008208053A (en) |
| KR (1) | KR100973782B1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014031377A (en) * | 2013-09-30 | 2014-02-20 | Masanori Somei | Composition containing n-acyl tryptamine |
| JP2014521698A (en) * | 2011-08-05 | 2014-08-28 | ステムテック インターナショナル， インコーポレイテッド | Skin care composition containing a combination of natural ingredients |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001079400A2 (en) | 2000-04-14 | 2001-10-25 | Mars Incorporated | Extraction of sterols from cocoa hulls |
| US20050036974A1 (en) * | 2003-07-17 | 2005-02-17 | L'oreal | Beta-endorphin activity in cosmetics and dermatology |
| JP4804805B2 (en) * | 2005-06-10 | 2011-11-02 | 横関油脂工業株式会社 | Cell activator, UV damage relieving agent and melanin production inhibitor |
-
2007
- 2007-02-23 JP JP2007044766A patent/JP2008208053A/en not_active Withdrawn
-
2008
- 2008-02-21 KR KR1020080016028A patent/KR100973782B1/en active IP Right Grant
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014521698A (en) * | 2011-08-05 | 2014-08-28 | ステムテック インターナショナル， インコーポレイテッド | Skin care composition containing a combination of natural ingredients |
| JP2014031377A (en) * | 2013-09-30 | 2014-02-20 | Masanori Somei | Composition containing n-acyl tryptamine |
Also Published As
| Publication number | Publication date |
|---|---|
| KR20080078583A (en) | 2008-08-27 |
| KR100973782B1 (en) | 2010-08-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A300 | Withdrawal of application because of no request for examination |
Free format text: JAPANESE INTERMEDIATE CODE: A300 Effective date: 20100511 |