CN115136904A

CN115136904A - Device for animal behavior experiment

Info

Publication number: CN115136904A
Application number: CN202210901233.3A
Authority: CN
Inventors: 刘伟; 宋远见
Original assignee: Xuzhou Medical University
Current assignee: Xuzhou Medical University
Priority date: 2022-07-28
Filing date: 2022-07-28
Publication date: 2022-10-04

Abstract

The invention relates to the technical field of medicine, and discloses a device for animal behavior experiments, which comprises a baffle, a fixed cross rod and a ring bin, wherein the baffle is fixedly arranged on the surface of the ring bin, a circular bottom plate is arranged at the bottom end of the ring bin, a closed arm bottom plate and an open arm bottom plate are sequentially and alternately arranged on the surface of the circular bottom plate, food placing boxes are respectively arranged at the end parts of the closed arm bottom plate and the open arm bottom plate, a feeding ring pipe is arranged below the baffle, and a connecting port is formed in the center of each food placing box in a penetrating manner. The invention can realize that the baffle of each arm is in black and white colors by arranging the matching of structures such as the black plate, the white plate, the first connecting block, the second connecting block and the like, and is convenient to assemble.

Description

Device for animal behavior experiment

Technical Field

The invention relates to the technical field of medicine, in particular to a device for animal behavior experiments.

Background

The experimental platform for the neurobehavioral of the small animals is the most basic supporting condition in the fields of neuroscience and brain science, and the amount of exercise and the cognitive ability of the animals can be evaluated by utilizing the exploratory exercise form of the experimental animals. The principle is that the animal anxiety state is investigated by utilizing the exploration characteristics of the animal on the novel environment and the fear psychology on the high-suspension open arm to form the contradiction conflict of the animal. By utilizing the position information provided by the far-end marker in the maze, the arm for placing food can be effectively determined, and the spatial reference memory and the working memory of the animal can be researched. The anxiety response of the big mouse and the small mouse can be measured statistically through the monitoring of the camera and the background assistance of the computer.

The traditional animal behavior experimental device mainly comprises the following defects:

(1) The cleaning is troublesome. The traditional animal behavior experiment device is more troublesome to clean after the experiment is finished, and the phenomenon that local areas are difficult to clean can occur.

(2) The recorded contrast is poor. In the experimental process, because of the color difference between black mice and white mice, the phenomenon of larger error caused by confusion during the recording and analyzing of the camera is also easily caused.

In view of the above, it is desirable to design a device for animal behavior experiments.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides a device for animal behavior experiments, which solves the problems in the background technology.

(II) technical scheme

In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a device for animal behavior experiment, includes baffle, fixed horizontal pole and ring storehouse, baffle fixed mounting is on the surface in ring storehouse, the bottom in ring storehouse is provided with circular bottom plate, circular bottom plate's surface has set gradually closed arm bottom plate and open arm bottom plate in turn, the tip of closed arm bottom plate and open arm bottom plate all is provided with food and places the box, the below of baffle is provided with puts in the ring canal, the central department that box was placed to food runs through and has seted up the connector, and food places the box and put in the ring canal and pass through the connector intercommunication, the top left and right sides of closed arm bottom plate all is provided with two first connecting blocks, the upper and lower both ends of baffle all are provided with two first connecting grooves, and first connecting groove is connected with first connecting block cooperation, the tip of baffle is provided with the planking, the second spread groove has all been seted up at the upper and lower both ends of planking, the end of closed arm bottom plate is provided with the second connecting block, and second connecting block and second spread groove cooperation and be connected.

Preferably, the baffle comprises a black plate and a white plate, and two first connecting sliding blocks are arranged at the end parts of the black plate and the white plate.

Preferably, two first connecting sliding grooves are formed in two sides of the surface of the outer plate, and the inner walls of the first connecting sliding grooves are movably connected with the first connecting sliding blocks.

Preferably, the surface of the feeding ring pipe is provided with a feeding pipe in a penetrating mode, the inside of the ring bin is provided with a placing bin in a penetrating mode, the inner surface of the placing bin is provided with eight communicating doors uniformly, and the inner walls of the top ends of the eight communicating doors are provided with lamp tubes.

Preferably, the number of the closed arm bottom plates and the number of the open arm bottom plates are four, and the inner part of the storage bin is communicated with the closed arm bottom plates and the open arm bottom plates through the communicating doors.

Preferably, fixed vertical rods are arranged at the left end and the right end of each fixed cross rod, fixed vertical rods are arranged at the centers of the two fixed vertical rods, and a top cross rod is fixedly arranged between the top ends of the two fixed vertical rods.

Preferably, the surface of top horizontal pole is provided with solid fixed ring, the center department of top horizontal pole is provided with the camera, the top cover of baffle is equipped with and covers cloth.

Preferably, two supporting cross rods are arranged below the fixed cross rod, a main rod is fixedly arranged at the center of each supporting cross rod, and the height of each main rod is 50cm.

Preferably, two the left and right ends of supporting the horizontal pole all are provided with a pair of spacing stand, the bottom of mobile jib is provided with the chassis.

Preferably, the baffle plate is connected with the closing arm bottom plate in a matched mode through a first connecting groove and a first connecting block, and the outer plate is connected with the closing arm bottom plate in a matched mode through a second connecting groove and a second connecting block.

(III) advantageous effects

The invention provides a device for animal behavior experiments, which has the following beneficial effects:

(1) The invention can realize that the baffle of each arm is in black and white colors by arranging the matching of structures such as the black plate, the white plate, the first connecting block, the second connecting block, the first connecting slide block and the like, and is also convenient to assemble.

(2) According to the invention, through the matching of the structures such as the feeding pipe, the feeding ring pipe, the connecting port and the food placing box, the feeding ring pipe is connected with the food placing box on each arm, so that experimenters can conveniently and rapidly add food into the food placing box without barriers.

(3) The invention can record and research the anxiety of the animal and the spatial reference memory and the working memory of the animal at the same time by matching the structures of the camera, the closed arm bottom plate, the open-close arm bottom plate and the like due to the combination of the contents of the elevated cross maze and the eight-arm maze.

(4) The invention can utilize the lamp tube to illuminate the whole device by arranging the lamp tube, the covering cloth and other structures so as to provide sampling light for the camera, and the covering cloth covers the device, so that the interference of the external environment can be reduced, and the authenticity and the objectivity of data can be improved.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is an enlarged view of the structure of FIG. 1 at A in accordance with the present invention;

FIG. 3 is a top view of the present invention;

FIG. 4 is an exploded view of the baffle structure of the present invention;

fig. 5 is a perspective view of the present invention.

In the figure: 1. a baffle plate; 2. fixing the cross bar; 3. fixing the vertical rod; 4. a fixing ring; 5. a top cross bar; 6. a camera; 7. covering cloth; 8. a support rail; 9. a limiting upright post; 10. a casting pipe; 11. a main rod; 12. a chassis; 13. looping a bin; 14. a lamp tube; 15. placing a bin; 16. fixing the longitudinal rod; 17. a closing arm base plate; 18. a food placing box; 19. an open arm bottom plate; 20. putting a ring pipe; 21. a connection port; 22. a first connecting groove; 23. a first connecting slide block; 24. a second connecting groove; 25. a first connecting chute; 26. an outer plate; 27. a first connection block; 28. a second connecting block; 29. a circular base plate; 101. a black plate; 102. a white board.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

As shown in fig. 1 to 5, the present invention provides a technical solution: a device for animal behavior experiments comprises a baffle 1, a fixed cross bar 2 and a ring bin 13, wherein the baffle 1 is fixedly arranged on the surface of the ring bin 13, a circular bottom plate 29 is arranged at the bottom end of the ring bin 13, a closed arm bottom plate 17 and an open arm bottom plate 19 are sequentially and alternately arranged on the surface of the circular bottom plate 29, food placing boxes 18 are respectively arranged at the end parts of the closed arm bottom plate 17 and the open arm bottom plate 19, a throwing ring pipe 20 is arranged below the baffle 1, a connecting port 21 is formed in the center of each food placing box 18 in a penetrating manner, the food placing boxes 18 are communicated with the throwing ring pipe 20 through the connecting ports 21, the throwing ring pipe 20 is communicated with the food placing boxes 18 on each arm, so that an experimenter can conveniently and rapidly add food into the food placing boxes 18 without obstacles, the interference to the experiments is avoided, two first connecting blocks 27 are respectively arranged on the left side and the right side of the top end of the closed arm bottom plate 17, the upper end and the lower end of the baffle 1 are both provided with two first connecting grooves 22, the first connecting grooves 22 are connected with first connecting blocks 27 in a matching way, the end part of the baffle 1 is provided with an outer plate 26, the upper end and the lower end of the outer plate 26 are both provided with second connecting grooves 24, the tail end of the closed arm bottom plate 17 is provided with a second connecting block 28, the second connecting block 28 is connected with the second connecting groove 24 in a matching way, the baffle 1 and the closed arm bottom plate 17 are connected in a matching way through the first connecting grooves 22 and the first connecting blocks 27, the outer plate 26 and the closed arm bottom plate 17 are connected in a matching way through the second connecting grooves 24 and the second connecting blocks 28, the baffle 1 and the outer plate 26 can be assembled on the closed arm bottom plate 17 more conveniently by utilizing the structures of the first connecting blocks 27, the second connecting blocks 28, the first connecting blocks 23 and the like, the baffle 1 comprises a black plate 101 and a white plate 102, the end parts of the black plate 101 and the white plate 102 are both provided with two first connecting blocks 23, two first connecting chutes 25 have been all seted up to the surface both sides of planking 26, and the inner wall of first connecting chute 25 and first connecting slider 23 swing joint, because baffle 1 is personally submitted black, and another personally submits white, can make the device's baffle 1 can directly turn over the experiment that is used for corresponding the mouse like this, its black mouse is with white face, white mouse is with black face to improve the contrast.

In the device, a camera 6 is used for monitoring the animal behaviors, and a collection card is connected with a computer to analyze and record various numerical values of animal behavior analysis software, wherein the analysis and record contents comprise the times of the animal entering an open arm, the arm opening activity, the arm closing time, the arm closing activity, the downward exploration time, the arm closing rear leg standing time and the central region activity. Number of times animals entered open arms: the number of times of entering any open arm base plate 19 is based on the four limbs of the animal entering the arm or 80% of the animal body entering the arm. Opening the arm: the duration of time that the animal's limb is fully or 80% of its body enters the open arm floor 19. The number of times of entering the closed arm is that the number of times of entering any closed arm bottom plate 17 is based on that the four limbs of the animal enter the arm or that 80% of the animal body enters the arm. Closing the arm to move: the duration of time that the animal limb is fully or 80% of its body is within the closed arm base 17. Number of downward explores: when the test animal is placed on the central storage compartment 15 area or the opening arm bottom plate 19, the number of times of the activity of exploring the edge of the head and shoulder extending out of the opening arm bottom plate 19 to the lower side of the maze is counted while holding the edge of the maze by the front paw. Number of times of arm closing and hind leg erection: also called closed arm support standing, the number of times the animal stands upright with the legs supporting the body after the front legs are raised on the closed arm base plate 17. Central zone activity: the central region refers to the region where the open arm bottom plate 19 and the closed arm bottom plate 17 are connected and crossed, and the movement path and the residence time of the experimental animal in the region.

Put in the surface of ring canal 20 and run through and be provided with and put in pipe 10, the inside of ring storehouse 13 runs through and has seted up and place storehouse 15, eight intercommunication doors have evenly been seted up to the internal surface of placing storehouse 15, the top inner wall department of eight intercommunication doors all is provided with fluorescent tube 14, closed arm bottom plate 17 and opening arm bottom plate 19's quantity is four, and the inside and closed arm bottom plate 17 and opening arm bottom plate 19 of placing storehouse 15 pass through the intercommunication door intercommunication, just so can utilize fluorescent tube 14 to illuminate whole device, provide the light of sampling for camera 6.

Both ends all are provided with fixed vertical pole 16 about fixed horizontal pole 2, the center department of two fixed vertical poles 16 all is provided with fixed pole setting 3, the fixed top horizontal pole 5 that is provided with between 3 tops of two fixed pole settings, the surface of top horizontal pole 5 is provided with solid fixed ring 4, the center department of top horizontal pole 5 is provided with camera 6, the top cover of baffle 1 is equipped with hiding cover cloth 7, it has hiding cover cloth 7 to cover in the top of the device, can reduce external environment's interference like this, with authenticity and the objectivity that improves data, the below of fixed horizontal pole 2 is provided with two and supports horizontal pole 8, the fixed mobile jib 11 that is provided with in center department of two support horizontal pole 8, the height of mobile jib 11 is 50cm, both ends all are provided with a pair of spacing stand 9 about two support horizontal pole 8, the bottom of mobile jib 11 is provided with chassis 12, the observation animal that can be better apart from ground 50cm highly explores the characteristic of new and the feared psychology of the arm of opening of height.

The animals are lightly placed in the central area of the annular bin 13, face to the direction of the opening arm bottom plate 19, meanwhile, the camera 6 is used for tracking and recording the track movement of the animals, the index is automatically calculated, the common experiment duration is 7 minutes, the experiment platform is arranged at the position 50cm away from the ground, and the animal conflict is formed by utilizing the exploration characteristics of the animals on the new and different environment and the fear psychology of the animals on the high-suspension opening arm, so that the anxiety state of the animals is investigated. And after the experiment is finished, taking out the experimental animal and putting the animal into a rearing cage, marking information of the animal after the experiment is finished, and cleaning the device by using alcohol and paper towels.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a device for animal behavior experiment, includes baffle (1), fixed horizontal pole (2) and ring storehouse (13), its characterized in that: the food storage box is characterized in that the baffle (1) is fixedly installed on the surface of the annular bin (13), a circular bottom plate (29) is arranged at the bottom end of the annular bin (13), a closed arm bottom plate (17) and an open arm bottom plate (19) are sequentially and alternately arranged on the surface of the circular bottom plate (29), food placing boxes (18) are arranged at the end portions of the closed arm bottom plate (17) and the open arm bottom plate (19), a throwing ring pipe (20) is arranged below the baffle (1), a connecting port (21) penetrates through the center of each food placing box (18), each food placing box (18) is communicated with the throwing ring pipe (20) through the corresponding connecting port (21), two first connecting blocks (27) are arranged on the left side and the right side of the top end of the closed arm bottom plate (17), two first connecting grooves (22) are arranged on the upper end and the lower end of the baffle (1), the first connecting grooves (22) are connected with the first connecting blocks (27) in a matched mode, an outer plate (26) is arranged on the end portion of the baffle (1), second connecting grooves (24) are arranged on the upper end and the lower end of the outer plate (26), and the second connecting blocks (28) are connected with the second connecting blocks (28) in a matched mode.

2. An apparatus for animal behaviour experiments according to claim 1, characterized in that: the baffle (1) comprises a black plate (101) and a white plate (102), and two first connecting sliding blocks (23) are arranged at the end parts of the black plate (101) and the white plate (102).

3. An apparatus for animal behavior experiments according to claim 2, characterized in that: two first connecting sliding grooves (25) are formed in two sides of the surface of the outer plate (26), and the inner wall of each first connecting sliding groove (25) is movably connected with the corresponding first connecting sliding block (23).

4. An apparatus for animal behavior experiments according to claim 1, characterized in that: the surface of the feeding ring pipe (20) is provided with a feeding pipe (10) in a penetrating mode, the inside of the ring bin (13) is provided with a placing bin (15) in a penetrating mode, eight communicating doors are evenly arranged on the inner surface of the placing bin (15), and lamp tubes (14) are arranged on the inner walls of the top ends of the eight communicating doors.

5. An apparatus for animal behavior experiments according to claim 4, characterized in that: the number of the closed arm bottom plates (17) and the number of the open arm bottom plates (19) are four, and the interior of the storage bin (15) is communicated with the closed arm bottom plates (17) and the open arm bottom plates (19) through the communication doors.

6. An apparatus for animal behavior experiments according to claim 1, characterized in that: fixed vertical pole (16) are all provided with at both ends about fixed horizontal pole (2), two the center department of fixed vertical pole (16) all is provided with fixed pole setting (3), fixedly between two fixed pole setting (3) tops is provided with top horizontal pole (5).

7. An apparatus for animal behavior experiments according to claim 6, characterized in that: the surface of top horizontal pole (5) is provided with solid fixed ring (4), the center department of top horizontal pole (5) is provided with camera (6), the top cover of baffle (1) is equipped with and covers cloth (7).

8. An apparatus for animal behavior experiments according to claim 1, characterized in that: two supporting cross rods (8) are arranged below the fixed cross rod (2), a main rod (11) is fixedly arranged at the center of each supporting cross rod (8), and the height of each main rod (11) is 50cm.

9. An apparatus for animal behavior experiments according to claim 8, characterized in that: the left end and the right end of the two supporting cross rods (8) are both provided with a pair of limiting upright posts (9), and the bottom end of the main rod (11) is provided with an underframe (12).

10. An apparatus for animal behavior experiments according to claim 1, characterized in that: the baffle plate (1) is in fit connection with the closing arm bottom plate (17) through a first connecting groove (22) and a first connecting block (27), and the outer plate (26) is in fit connection with the closing arm bottom plate (17) through a second connecting groove (24) and a second connecting block (28).