CN115093959A - Cell conveyer - Google Patents

Cell conveyer Download PDF

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Publication number
CN115093959A
CN115093959A CN202210835391.3A CN202210835391A CN115093959A CN 115093959 A CN115093959 A CN 115093959A CN 202210835391 A CN202210835391 A CN 202210835391A CN 115093959 A CN115093959 A CN 115093959A
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CN
China
Prior art keywords
cell
air
cell culture
circuit board
control circuit
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202210835391.3A
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Chinese (zh)
Inventor
姚明华
吴蓉
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Shanghai First Peoples Hospital
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Shanghai First Peoples Hospital
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Publication date
Application filed by Shanghai First Peoples Hospital filed Critical Shanghai First Peoples Hospital
Priority to CN202210835391.3A priority Critical patent/CN115093959A/en
Publication of CN115093959A publication Critical patent/CN115093959A/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
    • B65D81/02—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage
    • B65D81/05—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage maintaining contents at spaced relation from package walls, or from other contents
    • B65D81/127—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage maintaining contents at spaced relation from package walls, or from other contents using rigid or semi-rigid sheets of shock-absorbing material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/24—Gas permeable parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/46—Means for fastening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16F—SPRINGS; SHOCK-ABSORBERS; MEANS FOR DAMPING VIBRATION
    • F16F15/00—Suppression of vibrations in systems; Means or arrangements for avoiding or reducing out-of-balance forces, e.g. due to motion
    • F16F15/02—Suppression of vibrations of non-rotating, e.g. reciprocating systems; Suppression of vibrations of rotating systems by use of members not moving with the rotating systems
    • F16F15/04—Suppression of vibrations of non-rotating, e.g. reciprocating systems; Suppression of vibrations of rotating systems by use of members not moving with the rotating systems using elastic means
    • F16F15/06—Suppression of vibrations of non-rotating, e.g. reciprocating systems; Suppression of vibrations of rotating systems by use of members not moving with the rotating systems using elastic means with metal springs
    • F16F15/067—Suppression of vibrations of non-rotating, e.g. reciprocating systems; Suppression of vibrations of rotating systems by use of members not moving with the rotating systems using elastic means with metal springs using only wound springs
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16F—SPRINGS; SHOCK-ABSORBERS; MEANS FOR DAMPING VIBRATION
    • F16F15/00—Suppression of vibrations in systems; Means or arrangements for avoiding or reducing out-of-balance forces, e.g. due to motion
    • F16F15/02—Suppression of vibrations of non-rotating, e.g. reciprocating systems; Suppression of vibrations of rotating systems by use of members not moving with the rotating systems
    • F16F15/04—Suppression of vibrations of non-rotating, e.g. reciprocating systems; Suppression of vibrations of rotating systems by use of members not moving with the rotating systems using elastic means
    • F16F15/08—Suppression of vibrations of non-rotating, e.g. reciprocating systems; Suppression of vibrations of rotating systems by use of members not moving with the rotating systems using elastic means with rubber springs ; with springs made of rubber and metal
    • F16F15/085—Use of both rubber and metal springs

Abstract

The invention discloses a cell transportation device, which at least comprises: the box body at least comprises a horizontal clapboard inside, and the horizontal clapboard is provided with a through hole; the damping component is positioned in the lower cavity and comprises a plurality of springs with one ends fixed at the bottom of the lower cavity and a fixing plate fixed at the tail ends of the springs, and grooves are formed in the fixing plate; the cell culture bottle is clamped in the groove and provided with a ventilation bottle cap, the ventilation bottle cap is provided with an air inlet interface and an air outlet interface, and the air inlet interface and the air outlet interface are both internally provided with a layer of semipermeable membrane; and the air exchange system is fixed on the horizontal partition plate and comprises an air inlet assembly and an exhaust gas collecting assembly. The cell transportation device can realize automatic updating of gas in the transportation process, and the damping component can also prevent the cells from being damaged in the transportation process and simultaneously prevent the cells from being polluted.

Description

Cell conveyer
Technical Field
The invention relates to the field of cell transportation, in particular to a cell transportation device.
Background
With the continuous development of cell therapy industry, cell storage and culture mechanisms need to continuously transport cell samples to designated medical institutions, and cell culture provides important raw materials and experimental detection means for the development of research in the fields of medicine and biology, and cell transfer between laboratories also becomes an essential link in the research process. The safe and effective solution of the problem of living cell transportation is beneficial to the development of relevant fields such as laboratory basic scientific research, clinical cell therapy (immunotherapy, stem cell therapy, microbial therapy and the like), urine and blood detection and the like, is expected to improve the basic scientific research quality and the clinical treatment effect, reduces the scientific research and related treatment cost, and avoids unnecessary waste. Therefore, the cell transportation device and the transportation technique thereof are gradually drawing attention from the relevant personnel.
However, at present, cell transportation devices in different laboratories and different regions at home and abroad are mainly realized by filling cells into common cell culture bottles, and cannot maintain fresh and proper gas environment, so that the cells are in a poor state and even die greatly. In addition, the constant temperature state in the cell culture bottle is difficult to maintain, and the culture bottle is easy to be impacted to cause fragmentation, which is also a common problem in the cell transportation process.
Therefore, there is a need for a cell transportation device that can achieve gas environment renewal, maintain cell state, and improve cell survival rate during transportation.
Disclosure of Invention
The invention aims to solve the defects that the existing cell transportation device can not meet the requirements of the air environment updating in a culture bottle, the temperature in the bottle is difficult to keep at a proper temperature, the culture bottle is easy to be impacted and cracked, and the like.
In order to achieve the above object, the present invention provides a cell transport device comprising at least:
the box body is used for accommodating all the parts, at least one horizontal partition plate is arranged in the box body, the box body is divided into an upper cavity and a lower cavity, and through holes are formed in the horizontal partition plate;
the shock absorption component is positioned in the lower cavity and comprises a plurality of springs with one ends fixed at the bottom of the lower cavity and a fixing plate fixed at the tail ends of the springs, and grooves are formed in the fixing plate;
the cell culture bottles are clamped in the grooves and positioned between the fixed plate and the horizontal partition plate, the cell culture bottles are provided with air exchange bottle caps which are covered with the cell culture bottles, the air exchange bottle caps are respectively provided with an air inlet interface and an air outlet interface, and a layer of semipermeable membrane is arranged in each of the air inlet interface and the air outlet interface;
an air exchange system is fixed in on the horizontal partition plate, air exchange system contains an air intake subassembly and a waste gas collection subassembly, the subassembly that admits air passes the through-hole with the interface of admitting air of taking a breath bottle lid is through the pipeline intercommunication for carry fresh air to the cell culture bottle in, the waste gas collection subassembly passes the through-hole with the interface of giving vent to anger of taking a breath bottle lid is through the pipeline intercommunication, is used for discharging waste gas in the cell culture bottle.
Preferably, the cell transporting device further comprises a control circuit board, the gas inlet assembly is connected with a corresponding terminal of the control circuit board to control the entry of fresh gas, and the waste gas collecting assembly is connected with a corresponding terminal of the control circuit board to control the discharge of waste gas.
Preferably, the upper end of the box body is rotatably connected with an upper box cover through a hinge, and a fan is embedded in the upper box cover towards the inner side of the box body.
Preferably, the fan is connected with a wind speed adjusting module, and the wind speed adjusting module is connected with a corresponding terminal of the control circuit board.
Preferably, the fixed plate is provided with at least one temperature sensor electrically connected with the control circuit board, the temperature sensor is used for monitoring the temperature in the box in real time and sending a corresponding temperature signal to the control circuit board, and the control circuit board is used for responding to the temperature signal sent by the temperature sensor and then driving the wind speed adjusting module to adjust the rotating speed of the fan.
Preferably, the air inlet interface and the air outlet interface of the air exchange bottle cap are magnetic interfaces, so that the cell culture bottle and the air exchange system can be conveniently connected or separated.
Preferably, the side end of the box body is rotatably connected with a side box cover through a hinge.
Preferably, the fixing plate is made of any one material selected from ethylene-vinyl acetate copolymer, polyethylene and polyurethane.
Preferably, the cell transportation device further comprises a power supply electrically connected to the control circuit board for supplying power to the cell transportation device.
The invention has the beneficial effects that:
(1) the box body is divided into an upper cavity and a lower cavity, the upper cavity is provided with a gas replacement system, the lower cavity is provided with a damping component and a cell culture bottle, and the gas exchange system comprises a gas inlet component and a waste gas collection component, so that the gas in the cell culture bottle can be regularly and quantitatively updated and replaced in the cell transportation process, the gas in the cell culture bottle can be updated according to the growth rule of cells, the living gas environment of the cells in the cell culture bottle is further improved, and particularly, the circulating gas environment required by the cells is provided for long-distance cell transportation;
(2) the damping component is positioned in the lower cavity, and the fixing plate in the damping component is made of any one of ethylene-vinyl acetate copolymer, polyethylene and polyurethane, so that the vibration generated in the transportation process can be effectively absorbed, the collision between cells and a cell culture bottle is reduced, the shearing force generated between the cells is avoided, and the high survival rate of the cells is favorably maintained;
(3) the intelligent control technology is introduced into the cell transportation device, and the integrated structure ensures that the intelligent updating of the gas environment in the cell culture bottle and the intelligent control of the temperature of the cell culture bottle in the transportation process are realized when the cells are transported in a sealed cell culture bottle for a long distance;
(4) when the transportation arrives the destination, because the interface of admitting air and the interface of giving vent to anger of taking a breath bottle lid all are the design of formula of inhaling, can easily realize utilizing mechanical force with cell culture bottle and the separation of taking a breath bottle lid and air exchange system, guarantee that the cell culture bottle does not have the waste and reveals in the environment, pollution takes place when also having avoided the cell transportation.
Drawings
FIG. 1 is a schematic view of the overall structure of a cell transporter.
FIG. 2 is a schematic diagram of the structure of the case of the cell transporter.
FIG. 3 is a schematic view showing the structure of a shock-absorbing member of the cell transporter.
FIG. 4 is a schematic view of the structure of the ventilation system of the cell transporter.
Wherein, 1-box body, 11-horizontal clapboard, 111-through hole, 12-upper box cover, 13-side box cover, 14-fan, 15-handle, 2-cell culture bottle, 21-air exchanging bottle cover, 31-spring, 32-fixing plate, 33-temperature sensor, 41-inflatable body storage chamber, 42-inflatable body air pump, 43-inflatable body conveying pipeline, 44-waste gas collecting chamber, 45-waste gas collecting air pump, 46-waste gas collecting pipeline, 47-control circuit board, and 5-power supply.
Detailed Description
The technical solution of the present invention is further described below with reference to the accompanying drawings and examples.
The invention provides a cell transportation device, which at least comprises a box body, a damping part, a cell culture bottle, a ventilation system, a control circuit board and a power supply. The box body is at least internally provided with a horizontal clapboard which divides the box body into an upper cavity and a lower cavity, and the horizontal clapboard is provided with a through hole; the damping part is positioned in the lower cavity and comprises a plurality of springs with one ends fixed at the bottom of the lower cavity and a fixing plate fixed at the tail ends of the springs, and grooves are formed in the fixing plate; the cell culture bottle is clamped in the groove and provided with a ventilation bottle cap which is covered with the cell culture bottle, the ventilation bottle cap is provided with an air inlet interface and an air outlet interface, and a semi-permeable membrane is arranged in each of the air inlet interface and the air outlet interface; the air exchange system is fixed on the horizontal partition plate and comprises an air inlet assembly and an exhaust gas collecting assembly.
In some embodiments, the components are distributed in an integrated manner to achieve optimal transportation. As shown in fig. 1, the box body 1 at least contains a horizontal partition plate, in this embodiment, a horizontal partition plate 11 is contained, the box body 1 is divided into an upper cavity and a lower cavity, wherein the upper cavity is used for placing the replacement gas system, the lower cavity is used for placing the shock absorption component and the cell culture bottle 2, and the power supply 5 is placed at the rightmost side of the lower cavity and used for supplying power to the cell transportation device, and the power supply 5 further contains a charging interface for supplying power to the power supply.
As shown in fig. 2, the upper end and the side end of the box body 1 are rotatably connected with an upper box cover 12 and a side box cover 13 through hinges, the upper box cover 12 is opened and closed to facilitate replacement and collection of gas in the ventilation system, the side box cover 13 is opened and closed to facilitate pulling out and pushing in of the cell culture bottle 2, the side box cover 13 is of a magnetic type, when the cell culture bottle is transported to a destination, the cell culture bottle 2 and the ventilation bottle cover 21 can be easily separated from the ventilation system by only slightly opening the side box cover 13 and pulling out the cell culture bottle 2 outwards by using mechanical force and keeping the bottle mouth of the cell culture bottle upward, and at this time, cells transported to the destination can be subjected to next storage operation or other cell culture bottles needing to be transported can be replaced.
In some embodiments, in order to achieve the effect of damping vibration during cell transportation and avoid shearing force caused by mutual collision between cells, a damping member is fixedly disposed in the lower cavity of the box body 1. As shown in fig. 3, the damping member includes a spring 31 and a fixing plate 32, one end of the spring 31 is fixed to the bottom layer of the lower cavity by a screw, the fixing plate 32 is laid on the spring 31, a groove is formed on the fixing plate 32 for placing the cell culture bottle 2, the fixing plate 32 is made of any one of ethylene-vinyl acetate copolymer, polyethylene and polyurethane, in this embodiment, the ethylene-vinyl acetate copolymer is selected; in order to achieve a better damping effect, a plurality of springs 31 are generally disposed, and in this embodiment, 4 springs 31 are symmetrically disposed at four corners of the fixing plate 32, respectively, so as to avoid that an excessive shearing force is generated in the cell culture bottle 2 due to vibration during transportation, and then the cells are dead or damaged.
In some embodiments, in order to realize the exchange of gas in the cell culture bottle during the cell transportation process, as shown in fig. 3, a ventilation bottle cap 21 covering the cell culture bottle 2 is provided on the cell culture bottle 2, the ventilation bottle cap 21 is provided with a gas inlet port and a gas outlet port, the gas inlet port and the gas outlet port are respectively communicated with a gas inlet component and a waste gas collecting component of a ventilation system, so as to facilitate the realization of closed-loop update of the gas environment during the transportation process, the gas inlet port and the gas outlet port are both magnetic interfaces, so that when the transportation is completed and the cell culture bottle is detached, the spring 31 is pressed, the cell culture bottle 2 and the ventilation bottle cap 21 can be easily separated from the ventilation system, the ventilation bottle cap 21 can be taken out from the lower cavity of the box body 1 together with the cell culture bottle 2, and then the cell culture bottle 2 can be taken out by unscrewing the ventilation bottle cap 21. A semi-permeable membrane is arranged in each of the air inlet and the air outlet of the air exchange bottle cap 21, and the material is a super-hydrophobic polytetrafluoroethylene microporous membrane which is air-permeable and water-impermeable, mainly used for water proofing and air permeability, and used for preventing cells from being polluted.
In some embodiments, the gas is automatically refreshed during cell transport to maintain the cycle gas required during cell transport. As shown in fig. 1 and 4, a ventilation system is fixed on the upper chamber of the housing 1, the ventilation system includes an air intake assembly and a waste gas collection assembly, the air intake assembly passes through the through hole 111 and is communicated with the air intake port of the ventilation bottle cap 21 through a pipeline for delivering fresh air into the cell culture bottle 2, and the waste gas collection assembly passes through the through hole 111 and is communicated with the air outlet port of the ventilation bottle cap 21 through a pipeline for discharging waste gas in the cell culture bottle 2.
As shown in fig. 4, the ventilation system comprises an air intake module and an exhaust gas collecting module, the air intake module comprises an inflatable body storage chamber 41, an inflatable body delivery pipe 43 and an inflatable body air pump 42, the exhaust gas collecting module comprises an exhaust gas collecting chamber 44, an exhaust gas collecting delivery pipe 46 and an exhaust gas collecting air pump 45, and the inflatable body storage chamber 41, the inflatable body air pump 42, the exhaust gas collecting chamber 44 and the exhaust gas collecting air pump 45 are all provided with an air inlet port and an air outlet port. Wherein, the outlet port of the inflatable body storage chamber 41 is communicated with the inlet port of the inflatable body air pump 42 through the inflatable body conveying pipeline 43, and before transportation, the inlet port of the inflatable body storage chamber 41 is filled with the gas required by cell growth for providing gas in the transportation process; the air outlet interface of the inflatable body air pump 42 is communicated with the air inlet interface of the air exchanging bottle cap 21 through the inflatable body conveying pipeline 43; the air outlet interface of the air exchange bottle cap 21 is communicated with the air inlet interface of the waste gas collection air pump 45 through the waste gas collection conveying pipeline 46; the air outlet interface of the waste gas collecting air pump 45 is communicated with the air inlet interface of the waste gas collecting chamber 44 through the waste gas collecting and conveying pipeline 46; the inflatable body conveying pipeline 43 in the air inlet assembly penetrates through the through hole 111 to communicate the air inlet assembly in the upper cavity with the air inlet interface of the air exchange bottle cap 21 in the lower cavity, the waste gas collecting and conveying pipeline 46 in the waste gas collecting assembly penetrates through the through hole 111 to communicate the waste gas collecting assembly in the upper cavity with the air outlet interface of the air exchange bottle cap 21 in the lower cavity, on one hand, the through hole 111 can communicate the parts of the upper cavity and the lower cavity through pipelines, and on the other hand, the through hole 111 can also perform heat dissipation.
In some embodiments, to achieve intelligent control of gas during cell transport. As shown in fig. 4, the cell transportation device further comprises a control circuit board 47, the control circuit board 47 is connected with corresponding terminals of the inflatable air pump 42 and the waste gas collecting air pump 45 through wires, and respectively controls the switches of the inflatable air pump 42 and the waste gas collecting air pump 45 to realize the gas renewal in the cell culture bottle; the control circuit board 47 is electrically connected with the power supply 5 through a lead for supplying power to the cell transportation device.
In some embodiments, the temperature of the external environment of the cell culture bottle is monitored in real time, and the measured data is fed back to the control circuit board in real time. As shown in fig. 3, 5 temperature sensors 33 are uniformly arranged at the bottom of the fixing plate 32, respectively, for monitoring the external environment temperature of the cell culture bottle 2 in real time, and when the temperature sensors 33 monitor temperature data, the temperature data is immediately fed back to the control circuit board 47. Meanwhile, a fan 14 is embedded in one side of the box cover 12 on the box body 1 facing the inside of the box body 1, and the fan 14 is connected with a wind speed adjusting module which is connected with a corresponding terminal of the control circuit board 47 through a lead. After the temperature data is fed back to the control circuit board 47, the control circuit board 47 is configured to respond to the temperature signal sent by the temperature sensor 33 and then drive the wind speed adjusting module to adjust the rotation speed of the fan 14, so as to monitor and adjust the external environment temperature of the cell culture bottle 2 in real time and maintain the normal ambient gas temperature required by cell growth.
The growth temperature required for different types of cells, such as tumor cells, is different, and the transport environment needs to be maintained at 37.5 ℃ during transportation. The control circuit board 47 comprises a temperature setting button, which can set a specific temperature before cell transportation, when the external environment of the cell culture bottle 2 exceeds 37.5 ℃, the fan 14 is started, and when the temperature reaches 40 ℃, the rotating speed of the fan 14 reaches the maximum, so as to rapidly reduce the temperature; when the temperature drops to 37.5 ℃, the fan 14 can automatically stop; meanwhile, if the temperature changes between 37.5 ℃ and 40 ℃, the fan 14 can automatically adjust the rotating speed so as to ensure that the cells in the cell culture bottle 2 are in a constant temperature environment of 37.5 ℃ in real time. It is contemplated that if other types of cells are substituted, such as human cells, the temperature may be set to 36.5 ℃ prior to shipping, thereby allowing a constant 36.5 ℃ temperature during shipping.
The working principle of the cell transportation device provided by the invention is as follows: firstly, an operator loads cells and culture solution to be transported into a cell culture bottle 2 in advance, screws the cells and the culture solution with a ventilation bottle cap 21 in a clockwise direction, opens a side box cover 13, presses a spring 31, conveys the cell culture bottle 2 into a groove of a fixing plate 32 in a lower cavity of a box body 1, and fixes the cell culture bottle 2 through the groove; meanwhile, the upper case cover 12 is opened, corresponding gas is injected into the inflatable body storage chamber 41, then the upper case cover 12 and the side case cover 13 are closed in sequence, finally, the power supply 5 is opened, and all the components are transported after entering the working state: the corresponding end of the control circuit board 47 controls the inflatable air pump 42, so as to control the input of the fresh air in the cell culture bottle 2 by the air inlet component; the corresponding end of the control circuit board 47 controls the waste gas collecting air pump 45, and then controls the waste gas collecting component to collect the waste gas in the cell culture bottle 2. The corresponding required temperature is set according to the cell type before transportation, in the whole transportation process, the temperature sensor 33 sends a monitored temperature signal to the control circuit board 47, and the control circuit board 47 responds to the temperature signal sent by the temperature sensor 33 and then drives the wind speed adjusting module to adjust the rotating speed of the fan 14, so that the cells are in a specific constant temperature environment.
When the cells are transported to the designated position, firstly, the side box cover 13 is opened, the spring 31 is pressed, the cell culture bottle 2 is pulled out by mechanical force, the cell culture bottle 2 is vertically upwards unscrewed from the ventilation bottle cover 21 in the anticlockwise direction, the cell culture bottle 2 is placed at the designated position for convenient subsequent use, and finally, the power supply 5 is closed, so that the cells can be safely transported in a long distance in the specific constant temperature environment.
In summary, the cell transportation device of the present invention comprises a box body, a damping component, a cell culture bottle, a ventilation system, a control circuit board, and a power supply. Shock attenuation part can realize avoiding the production of shearing force and making the cell receive the damage in the cell transportation, and air exchange system can be so that the cell realizes fresh gas's input and the output of waste gas in the transportation, and when the structural design that integrates can guarantee that the cell carries out long distance transportation in sealed cell culture bottle, all be in the required specific constant temperature environment of cell growth, take place the pollution when avoiding the cell transportation, has improved the survival rate of cell in the transportation.
While the present invention has been described in detail with reference to the preferred embodiments, it should be understood that the above description should not be taken as limiting the invention. Various modifications and alterations to this invention will become apparent to those skilled in the art upon reading the foregoing description. Accordingly, the scope of the invention should be determined from the following claims.

Claims (9)

1. A cell transport device, comprising:
the box body is used for accommodating all the parts, at least one horizontal partition plate is arranged in the box body, the box body is divided into an upper cavity and a lower cavity, and through holes are formed in the horizontal partition plate;
the damping component is positioned in the lower cavity and comprises a plurality of springs with one ends fixed at the bottom of the lower cavity and a fixing plate fixed at the tail ends of the springs, and grooves are formed in the fixing plate;
the cell culture bottles are clamped in the grooves and positioned between the fixing plate and the horizontal partition plate, the cell culture bottles are provided with air exchange bottle caps which are covered with the cell culture bottles, the air exchange bottle caps are respectively provided with an air inlet interface and an air outlet interface, and a layer of semipermeable membrane is arranged in each of the air inlet interface and the air outlet interface; an air exchange system is fixed in on the horizontal partition plate, air exchange system contains an air intake subassembly and a waste gas collection subassembly, the subassembly that admits air passes the through-hole with the interface of admitting air of taking a breath bottle lid is through the pipeline intercommunication for carry fresh air to the cell culture bottle in, the waste gas collection subassembly passes the through-hole with the interface of giving vent to anger of taking a breath bottle lid is through the pipeline intercommunication, is used for discharging waste gas in the cell culture bottle.
2. The cell transporting device according to claim 1, further comprising a control circuit board, wherein the gas inlet module is connected to a corresponding terminal of the control circuit board to control the inlet of fresh gas, and the exhaust gas collecting module is connected to a corresponding terminal of the control circuit board to control the outlet of exhaust gas.
3. The cell transporting device according to claim 1, wherein an upper cover is pivotally connected to an upper end of the case body by a hinge, and a fan is embedded in the upper cover toward an inner side of the case body.
4. The cell transporting device according to claim 3, wherein the fan is connected with a wind speed adjusting module, and the wind speed adjusting module is connected with a corresponding terminal of the control circuit board.
5. The cell transportation device according to claim 4, wherein the fixing plate is provided with at least one temperature sensor electrically connected to the control circuit board, the temperature sensor is used for monitoring the temperature in the box body in real time and sending a corresponding temperature signal to the control circuit board, and the control circuit board is used for responding to the temperature signal sent by the temperature sensor and then driving the wind speed adjusting module to adjust the rotating speed of the fan.
6. The cell transportation device of claim 1, wherein the air inlet port and the air outlet port of the air exchange bottle cap are magnetic interfaces to facilitate connection and disconnection of the cell culture bottle to and from the air exchange system.
7. The cell transport apparatus of claim 1, wherein the side ends of the box are pivotally connected to side covers by hinges.
8. The cell transporting device according to claim 1, wherein the fixing plate is made of one selected from the group consisting of ethylene-vinyl acetate copolymer, polyethylene, and polyurethane.
9. The cell transport device of claim 1, further comprising a power supply electrically connected to the control circuit board for powering the cell transport device.
CN202210835391.3A 2022-07-15 2022-07-15 Cell conveyer Pending CN115093959A (en)

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CN202210835391.3A CN115093959A (en) 2022-07-15 2022-07-15 Cell conveyer

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CN202210835391.3A CN115093959A (en) 2022-07-15 2022-07-15 Cell conveyer

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CN115093959A true CN115093959A (en) 2022-09-23

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