CN115089642A - Preparation method of traditional Chinese medicine for treating type 2 diabetes - Google Patents
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Abstract
The invention belongs to the technical field of traditional Chinese medicines, and discloses a preparation method of a traditional Chinese medicine for treating type 2 diabetes, which comprises, by mass, 20-50 parts of a bitter gourd extract, 20-35 parts of a corydalis tuber extract, 15-30 parts of astragalus membranaceus, 15-30 parts of coptis chinensis, 15-30 parts of radix rehmanniae recen, 10-20 parts of poria cocos, 8-15 parts of a red peony root extract, 6-12 parts of cornus officinalis, 5-8 parts of oldenlandia diffusa, 3-5 parts of hairyvein agrimony, 2-4 parts of uncaria and sterilized deionized water. The method is simple and easy to implement, under the guidance of the traditional Chinese medicine theory and clinical experience, the raw materials and the proportion thereof are selected precisely, the traditional Chinese medicine for treating the type 2 diabetes is prepared by utilizing the mutual synergistic or antagonistic action, and the prepared traditional Chinese medicine has the effects of improving the insulin resistance of liver, fat and muscle tissues, protecting the function of islet beta cells and improving glycometabolism through the interaction and the synergistic cooperation of the raw materials, and can effectively control and treat the type 2 diabetes.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a preparation method of a traditional Chinese medicine for treating type 2 diabetes.
Background
Currently, diabetes is a major chronic disease characterized by hyperglycemia that is dangerous to human health. Hyperglycemia is caused by defects in insulin secretion or decreased sensitivity of the body to insulin, or both. Patients with type 1 diabetes have abnormal immune systems, and the autoimmune reaction is caused after infection of certain viruses such as coxsackie virus, rubella virus, mumps virus and the like, so that insulin beta cells are damaged, and the absolute deficiency of insulin is shown. Type 2 diabetes is mainly caused by excessive eating and reduced physical activity resulting in obesity, as well as genetic and environmental factors. Type 2 diabetes is common in the middle-aged and elderly, obese people, and is often accompanied by diseases such as hypertension, dyslipidemia, arteriosclerosis, and the like. The main pathological mechanism of type 2 diabetes is the development of insulin resistance, resulting in a relative deficiency of insulin. However, the existing medicines for treating type 2 diabetes in the market have long curative effect, slow effect and high cost in the actual use process, and are easy to generate side effects such as gastrointestinal tract reaction, liver and kidney function damage, hypoglycemia, weight gain and the like. Therefore, a new traditional Chinese medicine for treating type 2 diabetes and a preparation method thereof are needed to make up for the defects of the existing medicines.
Through the above analysis, the problems and defects of the prior art are as follows: the existing medicine for treating diabetes has long curative effect, slow effect and higher cost in the actual use process, and is easy to generate gastrointestinal tract reaction, liver and kidney function damage, hypoglycemia, weight gain and other side effects.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a preparation method of a traditional Chinese medicine for treating type 2 diabetes.
The invention is realized in such a way that a preparation method of a traditional Chinese medicine for treating type 2 diabetes comprises the following steps:
step one, obtaining fresh balsam pear, corydalis tuber decoction pieces and red paeony root; slicing fresh fructus Momordicae Charantiae, drying, pulverizing, adding water, extracting with low temperature water in extraction tank, concentrating under reduced pressure, separating, and adsorbing to obtain fructus Momordicae Charantiae extract;
step two, reflux-extracting the obtained rhizoma corydalis decoction pieces by using ethanol, concentrating, centrifuging, loading by using macroporous resin, eluting, concentrating and drying to obtain a rhizoma corydalis extract; soaking radix Paeoniae Rubra in water, decocting, extracting, adsorbing, eluting, distilling under reduced pressure to remove solvent, and drying to obtain radix Paeoniae Rubra extract;
weighing astragalus, coptis, radix rehmanniae, poria cocos, cornus officinalis, oldenlandia diffusa, hairyvein agrimony, uncaria, bitter gourd extract, corydalis tuber extract and red paeony root extract according to a proportion;
and step four, crushing and extracting the weighed astragalus, coptis, radix rehmanniae, poria, cornus officinalis, oldenlandia diffusa, hairyvein agrimony and uncaria, removing impurities, adding the balsam pear extract, the corydalis tuber extract, the red paeony root extract and sterilized deionized water, uniformly stirring, drying and granulating to obtain the traditional Chinese medicine for treating the type 2 diabetes.
Further, the preparation of the momordica charantia extract in the first step comprises the following steps:
(1) screening fresh bitter gourds, slicing the whole fruits, drying, and crushing to 80-150 meshes to obtain bitter gourd powder;
(2) uniformly mixing the balsam pear powder with water, and placing the mixture in an extraction tank for low-temperature water extraction to obtain an extracting solution;
(3) filtering the extractive solution under reduced pressure to obtain filtrate, concentrating the filtrate under reduced pressure to obtain extract of fructus Momordicae Charantiae, performing ceramic membrane separation with ceramic jar, and adsorbing impurities to obtain fructus Momordicae Charantiae extract.
Further, the bitter gourd powder in the step (2) and water are mixed according to the ratio of 1: and (3) uniformly mixing the components in a mass ratio of 2-5.
Further, concentrating the filtrate obtained in the step (3) under reduced pressure until the relative density is 1.15-1.20 to obtain the balsam pear extract.
Further, the preparation of the corydalis tuber extract in the second step comprises the following steps:
(1) taking rhizoma corydalis decoction pieces, performing reflux extraction for 1.5-2.0 h by using ethanol with the mass 6-15 times that of the rhizoma corydalis decoction pieces to obtain an extracting solution, concentrating the extracting solution, centrifuging, and taking supernate to prepare a supernatant;
(2) loading the pretreated macroporous resin into a column, loading the sample, and removing impurities; eluting with an elution solvent, and collecting the eluent after discarding the previous 0.5-1.5 BV of the eluent;
(3) concentrating the collected eluent, and drying to obtain rhizoma corydalis extract.
Further, the concentration of the ethanol is 45-90 wt%.
Further, the preparation of the red peony root extract in the third step comprises:
(1) taking red paeony root, adding water with the weight 8-10 times that of the red paeony root, soaking for 2-4 h, and then decocting; the decocting times are 3-5 times;
(2) sequentially extracting the decoction obtained in the step (1) by adopting petroleum ether, ethyl acetate and n-butanol, and collecting extract liquor;
(3) and adsorbing and eluting the extract liquor by macroporous resin, then distilling under reduced pressure to remove the solvent, and drying to obtain the red paeony root extract.
Further, the preparation of the traditional Chinese medicine for treating diabetes in the fourth step comprises the following steps:
(1) respectively crushing astragalus, coptis, radix rehmanniae, poria cocos, cornus officinalis, oldenlandia diffusa, hairyvein agrimony and uncaria, sieving by a 120-200-mesh sieve, and uniformly mixing to obtain mixed raw material powder;
(2) adding 8-12 times of ethanol by weight into the mixed raw material powder, extracting, concentrating under reduced pressure, performing ceramic membrane separation by using a ceramic tank, and adsorbing by using macroporous resin to remove impurities;
(3) and sequentially adding the balsam pear extract, the corydalis tuber extract, the red paeony root extract and the sterilized deionized water into the extract after impurity removal, uniformly stirring, drying and granulating to obtain the traditional Chinese medicine for treating diabetes.
The invention also aims to provide the traditional Chinese medicine for treating diabetes, which is prepared by the preparation method of the traditional Chinese medicine for treating diabetes and comprises, by mass, 20-50 parts of bitter gourd extract, 20-35 parts of corydalis tuber extract, 15-30 parts of astragalus, 15-30 parts of coptis chinensis, 15-30 parts of radix rehmanniae recen, 10-20 parts of poria cocos, 8-15 parts of red peony root extract, 6-12 parts of cornus officinalis, 5-8 parts of oldenlandia diffusa, 3-5 parts of hairyvein agrimony, 2-4 parts of uncaria and the balance of sterilized deionized water.
By combining all the technical schemes, the invention has the advantages and positive effects that: the preparation method of the traditional Chinese medicine for treating type 2 diabetes provided by the invention is simple and easy to implement, and under the guidance of the traditional Chinese medicine theory and clinical experience, the raw materials and the proportion thereof are selected precisely, and the traditional Chinese medicine for treating type 2 diabetes is prepared by utilizing the mutual synergistic or antagonistic action of the raw materials. The traditional Chinese medicine prepared by the invention has the functions of protecting pancreatic beta cells, stimulating beta cells to release insulin, improving insulin resistance and reducing blood sugar through the interaction and the synergistic cooperation of all the raw materials, and can effectively control hyperglycemia of a type 2 diabetes patient and improve symptoms of thirst, polydipsia and polyuria emaciation of the patient. And has less side effect. Wherein, the cornus and the tuckahoe can promote the brain health, the uncaria can relax the blood vessel and prevent the thrombosis, and the uncaria has obvious effect particularly on the brain part; the extract of Momordica charantia contains steroidal saponins such as momordin, insulinoids and alkaloids, which impart hypoglycemic activity to Momordica charantia. The main component of the momordicin has the functions of stimulating islet beta cells to release insulin and reducing fasting and postprandial blood sugar of patients, and plays an important role in the treatment of type 2 diabetes; the corydalis tuber extract has double effects of promoting insulin secretion and improving insulin resistance; radix astragali can inhibit hepatic glucose output by increasing hepatic glycogen synthesis and down-regulating gluconeogenic enzyme expression, thereby improving hepatic insulin resistance of type 2 diabetes; the coptis chinensis has the effects of clearing heat, drying dampness, purging fire, removing toxicity and thickening intestines and stomach, is matched with the pathogenesis of type 2 diabetes with the principal symptom of diabetes and qi deficiency, and berberine serving as a main component in the coptis chinensis can increase short-chain fatty acids in intestinal tracts, reduce the chronic inflammatory state of organisms and improve insulin resistance by adjusting the structure of intestinal flora so as to achieve the aim of treating type 2 diabetes; the dried rehmannia root has the effects of clearing heat and cooling blood, promoting the production of body fluid and quenching thirst, and can improve the function of islet beta cells, improve the resistance of peripheral tissues to insulin, regulate the self balance of cell glucose, reduce the activity of hepatic glucose-6-phosphatase and improve the disturbance of glycolipid metabolism; the radix Paeoniae Rubra extract can increase insulin sensitivity of fat and skeletal muscle cells and inhibit hepatic glucose output, thereby improving insulin resistance and reducing blood sugar; the oldenlandia diffusa and the hairyvein agrimony can inhibit the depolarization of islet beta cells, stimulate the islet beta cells to release insulin, inhibit the islet alpha cells to release glucagon, improve the sensitivity of peripheral tissues of type 2 diabetes patients to insulin and achieve the purpose of controlling blood sugar.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments of the present invention will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
Fig. 1 is a flow chart of a preparation method of a traditional Chinese medicine for treating diabetes provided by the embodiment of the invention.
Fig. 2 is a flow chart of a preparation method of an extract of momordica charantia provided by an embodiment of the present invention.
Fig. 3 is a flow chart of a preparation method of corydalis tuber extract according to an embodiment of the present invention.
FIG. 4 is a flow chart of a method for preparing a radix Paeoniae Rubra extract according to an embodiment of the present invention.
Fig. 5 is a flowchart of a method for preparing a traditional Chinese medicine for treating diabetes provided by the embodiment of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a preparation method of a traditional Chinese medicine for treating diabetes, and the invention is described in detail with reference to the accompanying drawings.
The traditional Chinese medicine for treating diabetes provided by the embodiment of the invention comprises, by mass, 20-50 parts of a bitter gourd extract, 20-35 parts of a corydalis tuber extract, 15-30 parts of astragalus membranaceus, 15-30 parts of coptis chinensis, 15-30 parts of radix rehmanniae recen, 10-20 parts of poria cocos, 8-15 parts of a red peony root extract, 6-12 parts of cornus officinalis, 5-8 parts of oldenlandia diffusa, 3-5 parts of hairyvein agrimony, 2-4 parts of uncaria and the balance of sterilized deionized water.
As shown in fig. 1, the preparation method of the traditional Chinese medicine for treating diabetes provided by the embodiment of the invention comprises the following steps:
s101, obtaining fresh bitter gourd, corydalis tuber decoction pieces and red paeony root; slicing fresh fructus Momordicae Charantiae, drying, pulverizing, adding water, extracting with low temperature water in extraction tank, concentrating under reduced pressure, separating, and adsorbing to obtain fructus Momordicae Charantiae extract;
s102, carrying out reflux extraction on the obtained rhizoma corydalis decoction pieces by using ethanol, concentrating, centrifuging, loading by using macroporous resin, eluting, concentrating and drying to obtain a rhizoma corydalis extract; soaking radix Paeoniae Rubra in water, decocting, extracting, adsorbing, eluting, distilling under reduced pressure to remove solvent, and drying to obtain radix Paeoniae Rubra extract;
s103, weighing radix astragali, rhizoma coptidis, radix rehmanniae, poria cocos, cornus officinalis, oldenlandia diffusa, hairyvein agrimony, uncaria, bitter gourd extract, rhizoma corydalis extract and red paeony root extract in proportion;
s104, crushing and extracting the weighed astragalus, coptis, radix rehmanniae, poria, cornus officinalis, oldenlandia diffusa, hairyvein agrimony and uncaria, removing impurities, adding the balsam pear extract, the corydalis tuber extract, the red paeony root extract and sterilized deionized water, uniformly stirring, drying and granulating to obtain the traditional Chinese medicine for treating diabetes.
As shown in fig. 2, the preparation of the momordica charantia extract in step S101 provided by the embodiment of the present invention includes:
s201, screening fresh bitter gourds, slicing the whole fruits, drying, and crushing to 80-150 meshes to obtain bitter gourd powder;
s202, uniformly mixing the bitter gourd powder with water, and placing the mixture in an extraction tank for low-temperature water extraction to obtain an extracting solution;
s203, filtering the extracting solution under reduced pressure to obtain filtrate, concentrating the filtrate under reduced pressure to obtain extract of the bitter gourd extract, performing ceramic membrane separation by using a ceramic pot, and adsorbing impurities to obtain the bitter gourd extract.
The bitter gourd powder and water provided by the embodiment of the invention are mixed according to the weight ratio of 1: and (3) uniformly mixing the components in a mass ratio of 2-5.
The filtrate provided by the embodiment of the invention is decompressed and concentrated to the relative density of 1.15-1.20, so as to obtain the extract of the balsam pear extract.
As shown in fig. 3, the preparation of the corydalis tuber extract in step S102 according to the embodiment of the present invention includes:
s301, taking corydalis tuber decoction pieces, performing reflux extraction for 1.5-2.0 hours by using ethanol with the mass 6-15 times that of the corydalis tuber decoction pieces to obtain an extracting solution, concentrating the extracting solution, centrifuging, and taking supernate to prepare a supernatant;
s302, loading the pretreated macroporous resin into a column, loading the sample, and removing impurities; eluting with an elution solvent, and collecting the eluent after discarding the previous 0.5-1.5 BV of the eluent;
s303, concentrating and drying the collected eluent to obtain the corydalis tuber extract.
The concentration of the ethanol provided by the embodiment of the invention is 45-90 wt%.
As shown in fig. 4, the preparation of the red peony root extract in step S102 provided by the embodiment of the present invention includes:
s401, taking red paeony root, adding water with the weight 8-10 times that of the red paeony root, soaking for 2-4 hours, and then decocting;
s402, sequentially extracting the decoction obtained in the step S401 by using petroleum ether, ethyl acetate and n-butanol, and collecting extract liquor;
and S403, adsorbing and eluting the extract liquor by macroporous resin, then carrying out reduced pressure distillation to remove the solvent, and drying to obtain the red paeony root extract.
The decoction frequency provided by the embodiment of the invention is 3-5 times.
As shown in fig. 5, the preparation of the traditional Chinese medicine for treating diabetes in step S104 according to the embodiment of the present invention includes:
s501, respectively crushing astragalus membranaceus, coptis chinensis, radix rehmanniae, poria cocos, cornus officinalis, oldenlandia diffusa, hairyvein agrimony and uncaria rhynchophylla, sieving the crushed materials with a 120-200-mesh sieve, and uniformly mixing the materials to obtain mixed raw material powder;
s502, adding 8-12 times of ethanol by weight into the mixed raw material powder, extracting, concentrating under reduced pressure, performing ceramic membrane separation by using a ceramic tank, and adsorbing by using macroporous resin to remove impurities;
s503, adding the balsam pear extract, the corydalis tuber extract, the red paeony root extract and the sterilized deionized water into the extract after impurity removal in sequence, stirring uniformly, drying and granulating to obtain the traditional Chinese medicine for treating diabetes.
The technical effects of the present invention will be further described below in conjunction with pharmacological analysis.
The pulp of dogwood fruit and the tuckahoe can promote the brain health, the uncaria can relax blood vessels and prevent thrombosis, and the uncaria has obvious effect particularly on brain parts.
The bitter gourd extract: contains steroid saponin such as momordicin, insulin-like peptide and alkaloid, which can endow Momordica charantia with hypoglycemic activity. The main component of the momordica charantia extract can stimulate the release of insulin and reduce the concentration of glucose in blood, so the momordica charantia extract can play a good role in controlling blood sugar of type 2 diabetes patients.
Corydalis tuber extract: has the double functions of promoting insulin secretion and improving insulin resistance.
Astragalus root: hepatic glucose output can be inhibited by increasing hepatic glycogen synthesis and down-regulating gluconeogenic enzyme expression, thereby improving hepatic insulin resistance in type 2 diabetes.
Coptis chinensis: has the effects of clearing heat, eliminating dampness, purging pathogenic fire, removing toxic substances, and thickening intestines and stomach, and is matched with the pathogenesis of type 2 diabetes with diabetes, qi deficiency, dryness-heat as the main target, berberine serving as the main component in coptis can increase short-chain fatty acids in intestinal tracts by adjusting the intestinal flora structure, reduce the chronic inflammation state of organisms, and improve insulin resistance, thereby realizing the purpose of treating type 2 diabetes.
Dried rehmannia root: has effects of clearing heat, cooling blood, promoting fluid production, quenching thirst, improving pancreatic islet beta cell function, improving insulin resistance of peripheral tissue, regulating cell glucose homeostasis, reducing hepatic glucose-6-phosphatase activity, and improving glycolipid metabolism disorder.
Red peony root extract: increasing sensitivity of fat and skeletal muscle cells to insulin, and inhibiting hepatic glucose output, thereby improving insulin resistance and reducing blood glucose.
The oldenlandia diffusa and the hairyvein agrimony can inhibit the depolarization of islet beta cells, stimulate the islet beta cells to release insulin, inhibit the islet alpha cells to release glucagon, improve the sensitivity of peripheral tissues of type 2 diabetes patients to insulin and achieve the purpose of controlling blood sugar.
The technical solution of the present invention is further illustrated by the following specific examples.
Example 1:
the preparation method of the traditional Chinese medicine for treating diabetes comprises the following steps:
step one, obtaining fresh balsam pear, corydalis tuber decoction pieces and red paeony root; slicing fresh fructus Momordicae Charantiae, drying, pulverizing, adding water, extracting with low temperature water in extraction tank, concentrating under reduced pressure, separating, and adsorbing to obtain fructus Momordicae Charantiae extract;
step two, reflux-extracting the obtained rhizoma corydalis decoction pieces by using ethanol, concentrating, centrifuging, loading by using macroporous resin, eluting, concentrating and drying to obtain a rhizoma corydalis extract; soaking radix Paeoniae Rubra in water, decocting, extracting, adsorbing, eluting, distilling under reduced pressure to remove solvent, and drying to obtain radix Paeoniae Rubra extract;
weighing 15 parts of astragalus membranaceus, 15 parts of coptis chinensis, 15 parts of radix rehmanniae recen, 10 parts of poria cocos, 6 parts of cornus officinalis, 5 parts of oldenlandia diffusa, 3 parts of hairyvein agrimony, 2 parts of uncaria, 20 parts of bitter gourd extract, 8 parts of corydalis tuber extract and 8 parts of red paeony root extract;
step four, crushing and extracting the weighed astragalus, coptis, radix rehmanniae, poria, cornus officinalis, oldenlandia diffusa, agrimony and uncaria, removing impurities, adding the balsam pear extract, the corydalis tuber extract, the red paeony root extract and sterilized deionized water, uniformly stirring, drying and granulating to obtain the traditional Chinese medicine for treating type 2 diabetes.
Example 2:
the preparation method of the traditional Chinese medicine for treating type 2 diabetes comprises the following steps:
step one, obtaining fresh balsam pear, corydalis tuber decoction pieces and red paeony root; slicing fresh fructus Momordicae Charantiae, drying, pulverizing, adding water, extracting with low temperature water in extraction tank, concentrating under reduced pressure, separating, and adsorbing to obtain fructus Momordicae Charantiae extract;
step two, reflux-extracting the obtained rhizoma corydalis decoction pieces by using ethanol, concentrating, centrifuging, loading by using macroporous resin, eluting, concentrating and drying to obtain a rhizoma corydalis extract; soaking radix Paeoniae Rubra in water, decocting, extracting, adsorbing, eluting, distilling under reduced pressure to remove solvent, and drying to obtain radix Paeoniae Rubra extract;
weighing 20 parts of astragalus membranaceus, 20 parts of coptis chinensis, 20 parts of radix rehmanniae recen, 15 parts of poria cocos, 10 parts of cornus officinalis, 7 parts of oldenlandia diffusa, 4 parts of hairyvein agrimonia herb and bud, 3 parts of uncaria, 30 parts of bitter gourd extract, 20 parts of corydalis tuber extract and 10 parts of red paeony root extract;
and step four, crushing and extracting the weighed astragalus, coptis, radix rehmanniae, poria, cornus officinalis, oldenlandia diffusa, hairyvein agrimony and uncaria, removing impurities, adding the balsam pear extract, the corydalis tuber extract, the red paeony root extract and sterilized deionized water, uniformly stirring, drying and granulating to obtain the traditional Chinese medicine for treating the diabetes.
Example 3:
the preparation method of the traditional Chinese medicine for treating diabetes comprises the following steps:
step one, obtaining fresh balsam pear, corydalis tuber decoction pieces and red paeony root; slicing fresh fructus Momordicae Charantiae, drying, pulverizing, adding water, extracting with low temperature water in extraction tank, concentrating under reduced pressure, separating, and adsorbing to obtain fructus Momordicae Charantiae extract;
step two, reflux-extracting the obtained rhizoma corydalis decoction pieces by using ethanol, concentrating, centrifuging, loading by using macroporous resin, eluting, concentrating and drying to obtain a rhizoma corydalis extract; soaking radix Paeoniae Rubra in water, decocting, extracting, adsorbing, eluting, distilling under reduced pressure to remove solvent, and drying to obtain radix Paeoniae Rubra extract;
weighing 30 parts of astragalus membranaceus, 30 parts of coptis chinensis, 30 parts of radix rehmanniae recen, 20 parts of poria cocos, 12 parts of cornus officinalis, 8 parts of oldenlandia diffusa, 5 parts of hairyvein agrimony, 4 parts of uncaria, 50 parts of bitter gourd extract, 35 parts of corydalis tuber extract and 15 parts of red paeony root extract;
and step four, crushing and extracting the weighed astragalus, coptis, radix rehmanniae, poria, cornus officinalis, oldenlandia diffusa, hairyvein agrimony and uncaria, removing impurities, adding the balsam pear extract, the corydalis tuber extract, the red paeony root extract and sterilized deionized water, uniformly stirring, drying and granulating to obtain the traditional Chinese medicine for treating the diabetes.
The above description is only for the purpose of illustrating the present invention and the appended claims are not to be construed as limiting the scope of the invention, which is intended to cover all modifications, equivalents and improvements that are within the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. A preparation method of a traditional Chinese medicine for treating type 2 diabetes is characterized by comprising the following steps:
step one, obtaining fresh balsam pear, corydalis tuber decoction pieces and red paeony root; slicing fresh fructus Momordicae Charantiae, drying, pulverizing, adding water, extracting with low temperature water in extraction tank, concentrating under reduced pressure, separating, and adsorbing to obtain fructus Momordicae Charantiae extract;
step two, reflux-extracting the obtained rhizoma corydalis decoction pieces by using ethanol, concentrating, centrifuging, loading by using macroporous resin, eluting, concentrating and drying to obtain a rhizoma corydalis extract; soaking radix Paeoniae Rubra in water, decocting, extracting, adsorbing, eluting, distilling under reduced pressure to remove solvent, and drying to obtain radix Paeoniae Rubra extract;
weighing astragalus, coptis, radix rehmanniae, poria cocos, cornus officinalis, oldenlandia diffusa, hairyvein agrimony, uncaria, bitter gourd extract, corydalis tuber extract and red paeony root extract according to a proportion;
step four, crushing and extracting the weighed astragalus, coptis, radix rehmanniae, poria, cornus officinalis, oldenlandia diffusa, agrimony and uncaria, removing impurities, adding the bitter gourd extract, the corydalis tuber extract, the red paeony root extract and sterilized deionized water, uniformly stirring, drying and granulating to obtain the traditional Chinese medicine for treating diabetes.
2. The method for preparing a traditional Chinese medicine for treating type 2 diabetes as claimed in claim 1, wherein the preparation of the momordica charantia extract in the first step comprises:
(1) screening fresh bitter gourds, slicing the whole fruits, drying, and crushing to 80-150 meshes to obtain bitter gourd powder;
(2) uniformly mixing the balsam pear powder with water, and placing the mixture in an extraction tank for low-temperature water extraction to obtain an extracting solution;
(3) filtering the extractive solution under reduced pressure to obtain filtrate, concentrating the filtrate under reduced pressure to obtain extract of fructus Momordicae Charantiae extract, performing ceramic membrane separation with ceramic tank, and adsorbing impurities to obtain fructus Momordicae Charantiae extract.
3. The preparation method of the traditional Chinese medicine for treating diabetes according to claim 2, wherein the bitter gourd powder and the water in the step (2) are mixed according to a ratio of 1: and (3) uniformly mixing the components in a mass ratio of 2-5.
4. The preparation method of the traditional Chinese medicine for treating type 2 diabetes according to claim 2, wherein the filtrate obtained in the step (3) is concentrated under reduced pressure until the relative density is 1.15-1.20, so as to obtain an extract of momordica charantia.
5. The method for preparing a Chinese medicine for treating type 2 diabetes according to claim 1, wherein the preparation of the corydalis tuber extract in the second step comprises:
(1) taking rhizoma corydalis decoction pieces, performing reflux extraction for 1.5-2.0 h by using ethanol with the mass 6-15 times that of the rhizoma corydalis decoction pieces to obtain an extracting solution, concentrating the extracting solution, centrifuging, and taking supernate to prepare a supernatant;
(2) loading the pretreated macroporous resin into a column, loading the sample, and removing impurities; eluting with an elution solvent, discarding the previous 0.5-1.5 BV of eluent, and collecting the eluent;
(3) concentrating the collected eluate, and drying to obtain rhizoma corydalis extract.
6. The method for preparing a traditional Chinese medicine for treating type 2 diabetes according to claim 5, wherein the concentration of ethanol is 45-90 wt%.
7. The method for preparing a traditional Chinese medicine for treating type 2 diabetes according to claim 1, wherein the preparation of the red peony root extract in the third step comprises:
(1) taking red paeony root, adding water with the weight 8-10 times that of the red paeony root, soaking for 2-4 h, and then decocting; the decocting times are 3-5 times;
(2) sequentially extracting the decoction obtained in the step (1) by adopting petroleum ether, ethyl acetate and n-butanol, and collecting extract liquor;
(3) and adsorbing and eluting the extract liquor by macroporous resin, then carrying out reduced pressure distillation to remove the solvent, and drying to obtain the red paeony root extract.
8. The method for preparing a traditional Chinese medicine for treating type 2 diabetes according to claim 1, wherein the preparation of the traditional Chinese medicine for treating diabetes in the fourth step comprises:
(1) respectively crushing astragalus, coptis, radix rehmanniae, poria cocos, cornus officinalis, oldenlandia diffusa, hairyvein agrimony and uncaria, sieving by a 120-200-mesh sieve, and uniformly mixing to obtain mixed raw material powder;
(2) adding 8-12 times of ethanol in parts by weight into the mixed raw material powder, extracting, concentrating under reduced pressure, performing ceramic membrane separation by using a ceramic tank, and adsorbing by adopting macroporous resin to remove impurities;
(3) and sequentially adding the balsam pear extract, the corydalis tuber extract, the red paeony root extract and the sterilized deionized water into the extract after impurity removal, uniformly stirring, drying and granulating to obtain the traditional Chinese medicine for treating type 2 diabetes.
9. The traditional Chinese medicine for treating type 2 diabetes, which is prepared by the preparation method of the traditional Chinese medicine for treating type 2 diabetes according to any one of claims 1 to 9, is characterized by comprising, by mass, 20 to 50 parts of a bitter gourd extract, 20 to 35 parts of a corydalis tuber extract, 15 to 30 parts of astragalus membranaceus, 15 to 30 parts of coptis chinensis, 15 to 30 parts of radix rehmanniae recen, 10 to 20 parts of poria cocos, 8 to 15 parts of a red peony root extract, 6 to 12 parts of cornus pulp, 5 to 8 parts of oldenlandia diffusa, 3 to 5 parts of hairyvein agrimony, 2 to 4 parts of uncaria and the balance of sterilized deionized water.
10. Use of the traditional Chinese medicine for treating type 2 diabetes according to claim 9 in the preparation of a medicament for treating, alleviating, preventing type 2 diabetes.
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