CN112704693A

CN112704693A - A Chinese medicinal extract composition for preventing and treating diabetes

Info

Publication number: CN112704693A
Application number: CN202011636950.5A
Authority: CN
Inventors: 吴杰; 吴海桥
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-12-31
Filing date: 2020-12-31
Publication date: 2021-04-27

Abstract

The invention relates to a traditional Chinese medicine extract composition capable of preventing and treating diabetes, which comprises the following components in parts by weight: 1-2 parts of ganoderma lucidum spore powder, 1-2 parts of bitter gourd extract, 1-2 parts of huperzia serrata extract, 2-3 parts of rhizoma corydalis extract, 1-2 parts of coptis chinensis extract, 1-2 parts of astragalus extract, 1-2 parts of salvia miltiorrhiza extract and 2-3 parts of red paeony root extract. The invention is based on the pathogenesis of diabetes which is disclosed by modern science, namely promoting the release of insulin and improving the insulin resistance. The traditional Chinese medicine extract composition has the effects of obviously blocking an ATP sensitive potassium ion channel, exciting islet beta cells and promoting insulin release, and can obviously improve insulin resistance of diabetes model animals, so that the blood sugar level of the diabetes model animals is reduced. The traditional Chinese medicine extract composition prepared by the invention can be used for preventing and treating type 2 diabetes. The traditional Chinese medicine extract composition can be prepared into capsules, tablets, granules, oral liquid, oral spray and the like.

Description

A Chinese medicinal extract composition for preventing and treating diabetes

Technical Field

The invention belongs to the technical field of traditional Chinese medicine extract formulas, and particularly relates to a traditional Chinese medicine extract composition capable of preventing and treating diabetes.

Background

Diabetes mellitus is a serious chronic disease characterized by hyperglycemia that endangers human health. Hyperglycemia is caused by a defect in insulin secretion or an impaired biological action, or both. Patients with type 1 diabetes have abnormal immune systems, and the autoimmune reaction is caused after infection of certain viruses such as coxsackie virus, rubella virus, mumps virus and the like, so that insulin beta cells are damaged, and the absolute deficiency of insulin is shown. Type 2 diabetes is mainly caused by excessive eating and by environmental factors such as obesity and heredity caused by reduced physical activity. Type 2 diabetes is common in the middle-aged and elderly, obese people, and is often accompanied by diseases such as hypertension, dyslipidemia, arteriosclerosis and the like. The main pathological mechanism of type 2 diabetes is the development of insulin resistance, resulting in a relative deficiency of insulin.

The control of blood sugar is the key to the treatment of diabetes, the treatment of diabetes is mainly carried out by western medicine at present, the main mode is oral hypoglycemic drugs or long-term insulin use, but the hypoglycemic drugs can cause the side effects of hypoglycemia, hyperlactacidosis, gastrointestinal discomfort, liver and kidney damage and the like on a human body, and the insulin can also cause the dependence and drug resistance of the human body and even cause hyperinsulinemia. Although a large number of traditional Chinese medicinal materials with the function of improving diabetes are recorded in the traditional Chinese medicine book, the advantages of the traditional Chinese medicine for treating diabetes are mainly reflected in the aspect of improving the symptoms of diabetic complications, and breakthrough progress cannot be made in the aspect of traditional Chinese medicine blood sugar reduction treatment, so that the treatment of diabetes by the traditional Chinese medicine is influenced. The reason may not be related to the blood sugar reducing effect of a single traditional Chinese medicine. Therefore, it is a very necessary matter to find a group of traditional Chinese medicinal materials which can better improve insulin resistance, control sugar and reduce blood sugar and have small side effects.

Disclosure of Invention

The invention aims to provide a traditional Chinese medicine extract composition capable of preventing and treating diabetes, and aims to solve the problems in the prior art.

A traditional Chinese medicine extract composition capable of preventing and treating diabetes comprises the following components in parts by weight: 1-2 parts of ganoderma lucidum spore powder, 1-2 parts of bitter gourd extract, 1-2 parts of huperzia serrata extract, 2-3 parts of rhizoma corydalis extract, 1-2 parts of coptis chinensis extract, 1-2 parts of astragalus extract, 1-2 parts of salvia miltiorrhiza extract and 2-3 parts of red paeony root extract.

Preferably, the traditional Chinese medicine extract composition capable of preventing and treating diabetes comprises the following components in parts by weight: ganoderma lucidum spore powder 1, balsam pear extract 1, huperzia serrata extract 1, corydalis tuber extract 2, coptis chinensis extract 1, astragalus extract 1, salvia miltiorrhiza extract 1 and red paeony root extract 2.

Preferably, the traditional Chinese medicine extract composition capable of preventing and treating diabetes can be prepared into capsules, tablets, granules, oral liquid or oral sprays.

Preferably, the traditional Chinese medicine extract composition capable of preventing and treating diabetes is applied to preparation of a medicine for preventing or treating diabetes.

Compared with the prior art, the design principle of the traditional Chinese medicine extract composition capable of preventing and treating diabetes is different from the traditional Chinese medicine theory, and is based on the pathogenesis of diabetes disclosed by modern science, namely, the promotion of insulin release and the improvement of insulin resistance. The pharmacological mechanism of each Chinese medicinal extract is as follows.

(1) Ganoderma lucidum spore powder: has the effects of improving the blood circulation of the pancreas, improving the physiological function and repair of pancreas islet beta cells, stimulating the secretion of insulin, reducing blood sugar and the like.

(2) The bitter gourd extract: contains steroid saponin such as momordicin, insulin-like peptide and alkaloid, which can endow Momordica charantia with hypoglycemic activity. Charantin stimulates the release of insulin and hinders the formation of glucose in the blood stream, a function which is particularly useful in the treatment of type 2 diabetes.

(3) Huperzia serrata extract: has the effect of improving neuropathy caused by diabetes.

(4) Corydalis tuber extract: has the double functions of promoting insulin secretion and improving insulin resistance.

(5) The coptis chinensis extract: the Coptidis rhizoma extract can improve glycolipid metabolism and insulin resistance.

(6) Astragalus extract: can inhibit gluconeogenesis by increasing liver glycogen synthesis and down-regulating gluconeogenic enzyme expression, thereby improving hepatic insulin resistance of type 2 diabetes.

(7) The red sage root extract: can improve diabetic microcirculation disturbance, thereby relieving liver, kidney, eye and neuropathy.

(8) Red peony root extract: promote glucose absorption and inhibit liver glucose regeneration, thereby improving insulin resistance and lowering blood sugar.

In the rat pancreas beta cell in-vitro experiment, the traditional Chinese medicine extract composition capable of preventing and treating diabetes has the effects of obviously blocking an ATP sensitive potassium ion channel, exciting islet beta cells and promoting insulin release, and in the whole diabetes animal model experiment, the traditional Chinese medicine extract composition can obviously improve the insulin resistance of animals so as to reduce the blood sugar level of the diabetes animals. The traditional Chinese medicine extract composition capable of preventing and treating diabetes can be used for preventing and treating type 2 diabetes. The traditional Chinese medicine extract composition capable of preventing and treating diabetes can be prepared into capsules, tablets, granules, oral liquid, oral spray and the like.

Drawings

FIG. 1 shows the effect of the composition of Chinese medicinal extracts for preventing and treating diabetes on the function of pancreatic beta cells of rats;

FIG. 2 is a graph showing the effect of the composition of Chinese medicinal extracts for the prevention and treatment of diabetes mellitus on ATP-sensitive potassium ion channels of rat pancreatic beta cells;

FIG. 3 shows the effect of the composition of Chinese medicinal extract for preventing and treating diabetes on the release of insulin from pancreatic beta cells of rats; p <0.05, p < 0.01;

FIG. 4 shows that the composition of Chinese medicinal extract for preventing and treating diabetes of the present invention lowers blood sugar level in diabetic rat model; p < 0.0001;

FIG. 5 shows the effect of the composition of Chinese medicinal extracts for preventing and treating diabetes on insulin receptor sensitivity of diabetic rats; p < 0.001;

FIG. 6 shows the effect of the composition of Chinese medicinal extracts for preventing and treating diabetes on insulin resistance of diabetic rats;

FIG. 7 shows the effect of the composition of Chinese medicinal extracts for preventing and treating diabetes on the insulin release level of rats.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings.

Embodiment 1 (for diabetes of more than moderate degree)

A traditional Chinese medicine extract composition capable of preventing and treating diabetes comprises the following components in parts by weight: 2 parts of ganoderma lucidum spore powder, 2 parts of balsam pear extract, 2 parts of huperzia serrata extract, 3 parts of corydalis tuber extract, 2 parts of coptis chinensis extract, 2 parts of astragalus extract, 2 parts of salvia miltiorrhiza extract and 3 parts of red paeony root extract.

Embodiment 2 (for incipient, mild diabetes)

A traditional Chinese medicine extract composition capable of preventing and treating diabetes comprises the following components in parts by weight: ganoderma lucidum spore powder 1, balsam pear extract 1, huperzia serrata extract 1, corydalis tuber extract 2, coptis chinensis extract 1, astragalus extract 1, salvia miltiorrhiza extract 1 and red paeony root extract 2.

The traditional Chinese medicine extract composition for preventing and treating diabetes mellitus in the embodiment 2 is uniformly mixed according to a proportion, and dissolved and prepared into three concentrations of 1%, 5% and 10% in an experiment.

Test and data (taking embodiment 2 as an example)

1. Effect on rat pancreatic beta cells

As can be seen from A in FIG. 1, under the conditions recorded by patch clamp and current clamp, rat pancreatic beta cells can cause cell membrane depolarization and cell excitation to generate action potential when perfused with high concentration glucose (22.5 mM). As can be seen from B in fig. 1: under the same experimental recording conditions, if a 10% concentration of the Chinese medicinal composition (ZYZH) is applied to pancreatic beta cells, it can also cause depolarization of cell membranes and excitation of cells, resulting in action potentials.

2. Effect on ATP-sensitive Potassium ion channels in rat pancreatic beta cells

As can be seen from a in fig. 2: in isolated rat pancreatic beta cells, ATP-sensitive potassium channels were opened by repeated triangular wave depolarization under patch clamp whole cell, voltage clamp recording conditions, and thus potassium channel currents were suppressed using ATP-sensitive potassium channel specific blockers (tolbutamides). As can be seen from B in fig. 2: the magnitude of the current can be enhanced with an ATP-sensitive potassium channel specific opener (diazolidine). As can be seen from C in fig. 2: under the same conditions, the current of ATP sensitive nail channel can be inhibited by using 10% of the traditional Chinese medicine composition. As can be seen from D in fig. 2: the composition of the extract of the chinese traditional medicine for preventing and treating diabetes mellitus of example 2 can inhibit the current of ATP-sensitive nail channel, and the inhibitory effect thereof is concentration-dependent.

3. Effect on insulin Release from rat pancreatic beta cells

It is shown from the left-hand bar of FIG. 3 that perfusing high concentration glucose (22.5mM) can increase insulin release. It is shown from the middle bar graph of fig. 3 that insulin release can also be increased if blockers of ATP-sensitive potassium channels are administered. As shown in the right-side column of fig. 3, the composition of the chinese herbal extract for diabetes prevention and treatment perfused with 10% of the composition also increases the release of insulin, indicating that the composition of the chinese herbal extract for diabetes prevention and treatment of example 2 has the effect of promoting the release of insulin by blocking ATP-sensitive nail channels.

4. Lowering blood glucose levels in a diabetic rat model

As can be seen from fig. 4, the composition of the chinese herbal extract for prevention and treatment of diabetes according to embodiment 2 (stippled column) has a lowering effect on blood glucose (empty column) of normal rats. In a diabetic rat model, the blood sugar level is shown as a black column, the traditional Chinese medicine extracts with different concentrations have different degrees of blood sugar reduction, wherein the traditional Chinese medicine composition with the concentration of 5 percent (left inclined column) and 10 percent (net column) has high statistical significance in reducing the blood sugar level of diabetic rats.

5. Effect on insulin receptor sensitivity in diabetic rats

As shown in FIG. 5, in the diabetic model rat, there was a decrease in insulin receptor sensitivity as shown by the black bars. After the Chinese medicinal composition is injected into the abdominal cavity, especially when the concentration reaches 5 percent (right oblique column) and 10 percent (net column), the sensitivity of insulin receptors can be obviously reduced.

6. Effect on insulin resistance in diabetic rats

As shown in fig. 6, diabetic rats developed significant Insulin Resistance (IR), which was found to be significantly reduced by 5% and 10% of the herbal extracts after treatment with the herbal composition.

7. Effect on rat insulin Release levels

As shown in fig. 7, the level of insulin secretion was increased in the diabetic rats because of the development of insulin resistance (black bars) compared to the normal rats. On the basis, 1% of the traditional Chinese medicine composition can further promote insulin release (vertical bars, the concentration of 1% does not reach the concentration for improving insulin resistance, see figure 6), but the promotion effect disappears along with the further increase of the concentration of the traditional Chinese medicine composition, and when the concentration of the traditional Chinese medicine composition reaches 5%, the insulin resistance is improved (see figure 6), so that the high insulin release level of the diabetic rat is reduced (right oblique bars).

The experiments show that the traditional Chinese medicine extract composition for preventing and treating diabetes can promote the release of insulin and improve insulin resistance, thereby effectively reducing and controlling the blood sugar level of diabetic rats, and can be used as a novel high-efficiency low-toxicity new scheme for preventing and treating diabetes with clear pharmacological action targets and mechanisms.

Claims

1. A traditional Chinese medicine extract composition capable of preventing and treating diabetes is characterized by comprising the following components in parts by weight: 1-2 parts of ganoderma lucidum spore powder, 1-2 parts of bitter gourd extract, 1-2 parts of huperzia serrata extract, 2-3 parts of rhizoma corydalis extract, 1-2 parts of coptis chinensis extract, 1-2 parts of astragalus extract, 1-2 parts of salvia miltiorrhiza extract and 2-3 parts of red paeony root extract.

2. The traditional Chinese medicine extract composition capable of preventing and treating diabetes as claimed in claim 1, which is characterized by comprising the following components in parts by weight: ganoderma lucidum spore powder 1, balsam pear extract 1, huperzia serrata extract 1, corydalis tuber extract 2, coptis chinensis extract 1, astragalus extract 1, salvia miltiorrhiza extract 1 and red paeony root extract 2.

3. The composition of claim 1, wherein the composition can be made into capsule, tablet, granule, oral liquid or oral spray.

4. The use of the composition of Chinese herbal extracts for the prevention and treatment of diabetes mellitus according to claim 1, in the preparation of a medicament for the prevention or treatment of diabetes mellitus.

5. The use according to claim 4, wherein the diabetes is type 2 diabetes.