CN115087736A - 一种使用阴离子交换剂的单链rna纯化方法 - Google Patents

一种使用阴离子交换剂的单链rna纯化方法 Download PDF

Info

Publication number
CN115087736A
CN115087736A CN202180013502.6A CN202180013502A CN115087736A CN 115087736 A CN115087736 A CN 115087736A CN 202180013502 A CN202180013502 A CN 202180013502A CN 115087736 A CN115087736 A CN 115087736A
Authority
CN
China
Prior art keywords
salt
anion exchanger
ssrna
salt solution
washing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202180013502.6A
Other languages
English (en)
Chinese (zh)
Inventor
P·S·加农
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sartorius Bia Separations doo
Original Assignee
Bia Separations doo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bia Separations doo filed Critical Bia Separations doo
Publication of CN115087736A publication Critical patent/CN115087736A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
CN202180013502.6A 2020-04-17 2021-04-16 一种使用阴离子交换剂的单链rna纯化方法 Pending CN115087736A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20170184.4A EP3896159B1 (en) 2020-04-17 2020-04-17 A method of single strand rna purification employing an anion exchanger
EP20170184.4 2020-04-17
PCT/EP2021/059911 WO2021209601A1 (en) 2020-04-17 2021-04-16 A method of single strand rna purification employing an anion exchanger

Publications (1)

Publication Number Publication Date
CN115087736A true CN115087736A (zh) 2022-09-20

Family

ID=70482246

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180013502.6A Pending CN115087736A (zh) 2020-04-17 2021-04-16 一种使用阴离子交换剂的单链rna纯化方法

Country Status (6)

Country Link
US (1) US20230142167A1 (https=)
EP (1) EP3896159B1 (https=)
JP (1) JP2023522526A (https=)
CN (1) CN115087736A (https=)
SI (1) SI3896159T1 (https=)
WO (1) WO2021209601A1 (https=)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US20160024141A1 (en) * 2013-03-15 2016-01-28 Modema Therapeutics, Inc. Ion exchange purification of mrna
CN107690481A (zh) * 2015-06-10 2018-02-13 凯杰有限公司 利用阴离子交换粒子分离细胞外核酸的方法
WO2018096179A1 (en) * 2016-11-28 2018-05-31 Curevac Ag Method for purifying rna

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7626017B2 (en) * 1997-10-31 2009-12-01 Pressure Biosciences, Inc. Pressure-enhanced extraction and purification
US20050059024A1 (en) * 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
GB2445442A (en) * 2006-09-26 2008-07-09 Ge Healthcare Bio Sciences Nucleic acid purification using anion exchange
EP2250182B1 (en) * 2008-02-07 2015-12-02 GE Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
CN103993002A (zh) * 2013-02-19 2014-08-20 百奥迈科生物技术有限公司 一种大规模合成长链rna药物的生产新工艺
SI3445850T1 (sl) * 2016-04-22 2021-12-31 BioNTech SE Postopki za zagotavljanje enoverižne RNA

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US20160024141A1 (en) * 2013-03-15 2016-01-28 Modema Therapeutics, Inc. Ion exchange purification of mrna
CN107690481A (zh) * 2015-06-10 2018-02-13 凯杰有限公司 利用阴离子交换粒子分离细胞外核酸的方法
WO2018096179A1 (en) * 2016-11-28 2018-05-31 Curevac Ag Method for purifying rna

Also Published As

Publication number Publication date
JP2023522526A (ja) 2023-05-31
WO2021209601A1 (en) 2021-10-21
EP3896159B1 (en) 2025-05-28
SI3896159T1 (sl) 2025-08-29
US20230142167A1 (en) 2023-05-11
EP3896159A1 (en) 2021-10-20

Similar Documents

Publication Publication Date Title
JP7783831B2 (ja) 一本鎖ｒｎａの精製方法
ES3034521T3 (en) A method of single-stranded rna purification
KR102919038B1 (ko) 음이온 교환 크로마토그래피를 이용한 플라스미드 dna와 같은 생물학적 분자의 정제 공정
CN116261483B (zh) 多模式金属亲和加工衣壳
JP6385374B2 (ja) タンパク質製剤からエンドトキシンを除去するための物質および方法
RU2011112989A (ru) Способы очистки однодоменных антигенсвязывающих молекул
Sinha et al. Analysis and purification of synthetic nucleic acids using HPLC
CN116234619A (zh) 宿主细胞dna的金属亲和提取
CN115087736A (zh) 一种使用阴离子交换剂的单链rna纯化方法
JPWO2017115652A1 (ja) 分離分析方法
WO2001037987A1 (en) A method for selective removal of a substance from samples containing compounds having nucleic acid structure
US20260015603A1 (en) A process and chromatography material for chromatography recovery of nucleic acid molecules
EP3957378B1 (en) Multimodal metal affinity processing aav capsids
DE60305075T2 (de) Isolierung von antisense-oligonukleotiden
HK40058215A (zh) 采用阴离子交换色谱法的生物分子(如质粒dna)纯化工艺
WO2026087772A1 (en) Multivalent halide salts for use in hydrophobic interaction chromatography
CN116348598A (zh) 通过疏水相互作用色谱法改善dna质粒制剂中rna和污染物的去除
JP2023522526A5 (https=)

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
CB02 Change of applicant information

Address after: Agidofsina, Slovenia

Applicant after: Sedolis Biya Separation Co.,Ltd.

Address before: Agidofsina, Slovenia

Applicant before: BIA SEPARATIONS D O O

CB02 Change of applicant information