SI3896159T1 - Metoda čiščenja enoverižne rnk z uporabo anionskega izmenjevalca - Google Patents

Metoda čiščenja enoverižne rnk z uporabo anionskega izmenjevalca

Info

Publication number
SI3896159T1
SI3896159T1 SI202030627T SI202030627T SI3896159T1 SI 3896159 T1 SI3896159 T1 SI 3896159T1 SI 202030627 T SI202030627 T SI 202030627T SI 202030627 T SI202030627 T SI 202030627T SI 3896159 T1 SI3896159 T1 SI 3896159T1
Authority
SI
Slovenia
Prior art keywords
single strand
anion exchanger
strand rna
rna purification
purification employing
Prior art date
Application number
SI202030627T
Other languages
English (en)
Slovenian (sl)
Inventor
Peter S. Gangnon
Original Assignee
Sartorius Bia Separations D.O.O.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sartorius Bia Separations D.O.O. filed Critical Sartorius Bia Separations D.O.O.
Publication of SI3896159T1 publication Critical patent/SI3896159T1/sl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
SI202030627T 2020-04-17 2020-04-17 Metoda čiščenja enoverižne rnk z uporabo anionskega izmenjevalca SI3896159T1 (sl)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP20170184.4A EP3896159B1 (en) 2020-04-17 2020-04-17 A method of single strand rna purification employing an anion exchanger

Publications (1)

Publication Number Publication Date
SI3896159T1 true SI3896159T1 (sl) 2025-08-29

Family

ID=70482246

Family Applications (1)

Application Number Title Priority Date Filing Date
SI202030627T SI3896159T1 (sl) 2020-04-17 2020-04-17 Metoda čiščenja enoverižne rnk z uporabo anionskega izmenjevalca

Country Status (6)

Country Link
US (1) US20230142167A1 (https=)
EP (1) EP3896159B1 (https=)
JP (1) JP2023522526A (https=)
CN (1) CN115087736A (https=)
SI (1) SI3896159T1 (https=)
WO (1) WO2021209601A1 (https=)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US7626017B2 (en) * 1997-10-31 2009-12-01 Pressure Biosciences, Inc. Pressure-enhanced extraction and purification
US20050059024A1 (en) * 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
GB2445442A (en) * 2006-09-26 2008-07-09 Ge Healthcare Bio Sciences Nucleic acid purification using anion exchange
EP2250182B1 (en) * 2008-02-07 2015-12-02 GE Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
CN103993002A (zh) * 2013-02-19 2014-08-20 百奥迈科生物技术有限公司 一种大规模合成长链rna药物的生产新工艺
US10590161B2 (en) * 2013-03-15 2020-03-17 Modernatx, Inc. Ion exchange purification of mRNA
JP6787916B2 (ja) * 2015-06-10 2020-11-18 キアゲン ゲーエムベーハー アニオン交換粒子を使用する細胞外核酸を単離する方法
SI3445850T1 (sl) * 2016-04-22 2021-12-31 BioNTech SE Postopki za zagotavljanje enoverižne RNA
WO2018096179A1 (en) * 2016-11-28 2018-05-31 Curevac Ag Method for purifying rna

Also Published As

Publication number Publication date
JP2023522526A (ja) 2023-05-31
WO2021209601A1 (en) 2021-10-21
EP3896159B1 (en) 2025-05-28
CN115087736A (zh) 2022-09-20
US20230142167A1 (en) 2023-05-11
EP3896159A1 (en) 2021-10-20

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