JP2023522526A - アニオン交換体を用いる一本鎖ｒｎａ精製方法 - Google Patents

アニオン交換体を用いる一本鎖ｒｎａ精製方法 Download PDF

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JP2023522526A
JP2023522526A JP2022543191A JP2022543191A JP2023522526A JP 2023522526 A JP2023522526 A JP 2023522526A JP 2022543191 A JP2022543191 A JP 2022543191A JP 2022543191 A JP2022543191 A JP 2022543191A JP 2023522526 A JP2023522526 A JP 2023522526A
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anion exchanger
salt
ssrna
wash
salt solution
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JP2023522526A5 (https=
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スタンリー ギャニオン、ピーター
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ザルトリウス ビーアイエー セパレーションズ ディー．オー．オー．
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Publication of JP2023522526A publication Critical patent/JP2023522526A/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
JP2022543191A 2020-04-17 2021-04-16 アニオン交換体を用いる一本鎖ｒｎａ精製方法 Pending JP2023522526A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20170184.4A EP3896159B1 (en) 2020-04-17 2020-04-17 A method of single strand rna purification employing an anion exchanger
EP20170184.4 2020-04-17
PCT/EP2021/059911 WO2021209601A1 (en) 2020-04-17 2021-04-16 A method of single strand rna purification employing an anion exchanger

Publications (2)

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JP2023522526A true JP2023522526A (ja) 2023-05-31
JP2023522526A5 JP2023522526A5 (https=) 2024-04-26

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JP2022543191A Pending JP2023522526A (ja) 2020-04-17 2021-04-16 アニオン交換体を用いる一本鎖ｒｎａ精製方法

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US (1) US20230142167A1 (https=)
EP (1) EP3896159B1 (https=)
JP (1) JP2023522526A (https=)
CN (1) CN115087736A (https=)
SI (1) SI3896159T1 (https=)
WO (1) WO2021209601A1 (https=)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050019814A1 (en) * 1997-10-31 2005-01-27 Bbi Bioseq, Inc., A Massachusetts Corporation Pressure-enhanced extraction and purification
JP2007500008A (ja) * 2003-07-25 2007-01-11 アンビオン インコーポレーティッド 小型ｒｎａ分子を単離するための方法および組成物
WO2009100172A1 (en) * 2008-02-07 2009-08-13 Ge Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
JP2010504738A (ja) * 2006-09-26 2010-02-18 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 陰イオン交換を用いる核酸精製方法
WO2014144767A1 (en) * 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
CN103993002A (zh) * 2013-02-19 2014-08-20 百奥迈科生物技术有限公司 一种大规模合成长链rna药物的生产新工艺
JP6787916B2 (ja) * 2015-06-10 2020-11-18 キアゲン ゲーエムベーハー アニオン交換粒子を使用する細胞外核酸を単離する方法
SI3445850T1 (sl) * 2016-04-22 2021-12-31 BioNTech SE Postopki za zagotavljanje enoverižne RNA
WO2018096179A1 (en) * 2016-11-28 2018-05-31 Curevac Ag Method for purifying rna

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050019814A1 (en) * 1997-10-31 2005-01-27 Bbi Bioseq, Inc., A Massachusetts Corporation Pressure-enhanced extraction and purification
JP2007500008A (ja) * 2003-07-25 2007-01-11 アンビオン インコーポレーティッド 小型ｒｎａ分子を単離するための方法および組成物
JP2010504738A (ja) * 2006-09-26 2010-02-18 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 陰イオン交換を用いる核酸精製方法
WO2009100172A1 (en) * 2008-02-07 2009-08-13 Ge Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
WO2014144767A1 (en) * 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BAUTZ EK. ET AL.: "Gene-specific messenger RNA: isolation by the deletion method", SCIENCE, vol. vol. 151(3708), JPN6025019511, 1966, pages 328 - 330, ISSN: 0005597078 *

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Publication number Publication date
WO2021209601A1 (en) 2021-10-21
EP3896159B1 (en) 2025-05-28
SI3896159T1 (sl) 2025-08-29
CN115087736A (zh) 2022-09-20
US20230142167A1 (en) 2023-05-11
EP3896159A1 (en) 2021-10-20

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