CN115087732B - 来自人T细胞来源的iPS细胞的细胞毒性T细胞 - Google Patents
来自人T细胞来源的iPS细胞的细胞毒性T细胞 Download PDFInfo
- Publication number
- CN115087732B CN115087732B CN202180012822.XA CN202180012822A CN115087732B CN 115087732 B CN115087732 B CN 115087732B CN 202180012822 A CN202180012822 A CN 202180012822A CN 115087732 B CN115087732 B CN 115087732B
- Authority
- CN
- China
- Prior art keywords
- cells
- hla
- cell
- ips
- human
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K40/00—Cellular immunotherapy
- A61K40/10—Cellular immunotherapy characterised by the cell type used
- A61K40/11—T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K40/00—Cellular immunotherapy
- A61K40/10—Cellular immunotherapy characterised by the cell type used
- A61K40/15—Natural-killer [NK] cells; Natural-killer T [NKT] cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K40/00—Cellular immunotherapy
- A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
- A61K40/46—Viral antigens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
- C12N5/0638—Cytotoxic T lymphocytes [CTL] or lymphokine activated killer cells [LAK]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2207/00—Modified animals
- A01K2207/12—Animals modified by administration of exogenous cells
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/105—Murine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
- A61K2239/31—Indexing codes associated with cellular immunotherapy of group A61K40/00 characterized by the route of administration
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
- A61K2239/38—Indexing codes associated with cellular immunotherapy of group A61K40/00 characterised by the dose, timing or administration schedule
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
- A61K2239/46—Indexing codes associated with cellular immunotherapy of group A61K40/00 characterised by the cancer treated
- A61K2239/59—Reproductive system, e.g. uterus, ovaries, cervix or testes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/11—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from blood or immune system cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18811—Sendai virus
- C12N2760/18841—Use of virus, viral particle or viral elements as a vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18811—Sendai virus
- C12N2760/18841—Use of virus, viral particle or viral elements as a vector
- C12N2760/18843—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Veterinary Medicine (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020-019548 | 2020-02-07 | ||
| JP2020019548 | 2020-02-07 | ||
| PCT/JP2021/004232 WO2021157685A1 (ja) | 2020-02-07 | 2021-02-05 | ヒトT細胞由来iPS細胞由来の細胞傷害性T細胞 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115087732A CN115087732A (zh) | 2022-09-20 |
| CN115087732B true CN115087732B (zh) | 2024-07-30 |
Family
ID=77199357
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202180012822.XA Active CN115087732B (zh) | 2020-02-07 | 2021-02-05 | 来自人T细胞来源的iPS细胞的细胞毒性T细胞 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20230071538A1 (https=) |
| EP (1) | EP4101924A4 (https=) |
| JP (1) | JP7743980B2 (https=) |
| CN (1) | CN115087732B (https=) |
| CA (1) | CA3170361A1 (https=) |
| WO (1) | WO2021157685A1 (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023037544A1 (ja) * | 2021-09-13 | 2023-03-16 | 公益財団法人京都大学iPS細胞研究財団 | 多能性幹細胞の製造方法 |
| WO2025111589A1 (en) * | 2023-11-22 | 2025-05-30 | Medici Therapeutics, Inc. | Compositions and methods for enhancing drug resistance in cells |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013176197A1 (ja) * | 2012-05-22 | 2013-11-28 | 国立大学法人 東京大学 | 抗原特異的t細胞の製造方法 |
| CN111164204A (zh) * | 2017-10-06 | 2020-05-15 | 赛雅思株式会社 | 来自iPS细胞的具有遗传多样性的T细胞群体的制备方法 |
| CN116515740A (zh) * | 2023-03-28 | 2023-08-01 | 南京艾尔普再生医学科技有限公司 | 一种低免疫原性iPSC细胞株 |
| WO2023247727A2 (en) * | 2022-06-23 | 2023-12-28 | Ehninger Dr Armin | Engineered human t cells comprising a switchable chimeric antigen cell surface receptor and methods for generating them |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070036773A1 (en) * | 2005-08-09 | 2007-02-15 | City Of Hope | Generation and application of universal T cells for B-ALL |
| JPWO2011096482A1 (ja) * | 2010-02-03 | 2013-06-13 | 国立大学法人 東京大学 | 多能性幹細胞を用いた免疫機能再建法 |
| FI2838548T3 (fi) * | 2012-04-17 | 2023-10-02 | Univ Washington Through Its Center For Commercialization | HLA:n luokan II puutteelliset solut, HLA:n luokan II puutteelliset solut, jotka kykenevät ilmentämään HLA:n luokan II proteiineja, ja niiden käytöt |
| US20170128556A1 (en) * | 2013-12-26 | 2017-05-11 | Thyas Co. Ltd. | Immunotherapy using t precursor cells derived from pluripotent stem cells having rearranged t cell receptor genes |
| JP6715482B2 (ja) * | 2014-07-18 | 2020-07-01 | 国立大学法人京都大学 | 多能性幹細胞から免疫細胞療法用t細胞を誘導する方法 |
| WO2019160077A1 (ja) * | 2018-02-16 | 2019-08-22 | 国立大学法人京都大学 | 低抗原性細胞の製造方法 |
| CA3177757A1 (en) * | 2018-02-16 | 2019-08-22 | Kite Pharma, Inc. | Modified pluripotent stem cells and methods of making and use |
| CA3162755A1 (en) * | 2019-11-25 | 2021-06-03 | Kyoto University | T-cell master cell bank |
-
2021
- 2021-02-05 JP JP2021575877A patent/JP7743980B2/ja active Active
- 2021-02-05 CN CN202180012822.XA patent/CN115087732B/zh active Active
- 2021-02-05 WO PCT/JP2021/004232 patent/WO2021157685A1/ja not_active Ceased
- 2021-02-05 EP EP21750181.6A patent/EP4101924A4/en active Pending
- 2021-02-05 US US17/760,245 patent/US20230071538A1/en active Pending
- 2021-02-05 CA CA3170361A patent/CA3170361A1/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013176197A1 (ja) * | 2012-05-22 | 2013-11-28 | 国立大学法人 東京大学 | 抗原特異的t細胞の製造方法 |
| CN111164204A (zh) * | 2017-10-06 | 2020-05-15 | 赛雅思株式会社 | 来自iPS细胞的具有遗传多样性的T细胞群体的制备方法 |
| WO2023247727A2 (en) * | 2022-06-23 | 2023-12-28 | Ehninger Dr Armin | Engineered human t cells comprising a switchable chimeric antigen cell surface receptor and methods for generating them |
| CN116515740A (zh) * | 2023-03-28 | 2023-08-01 | 南京艾尔普再生医学科技有限公司 | 一种低免疫原性iPSC细胞株 |
Non-Patent Citations (7)
| Title |
|---|
| Advances in allogeneic cancer cell therapy and future perspectives on ‘Off-the shelf’ T cell therapy using iPSC technology and gene editing;Yoshiki Furukawa等;Cells;第11卷;第1-15页 * |
| Current status and future perspectives of HLA-edited induced pluripotent stem cells;Keiko Koga等;Inflamm Regen;第40卷;第1-6页 * |
| Generation of hypoimmunogenic T cells from genetically engineered allogeneic human induced pluripotent stem cells;Bo Wang等;Nature Biomedical Engineering;20210517;第05卷;第429-440页 * |
| iPSC-derived hypoimmunogenic tissue resident memory T cells mediate robust anti-tumor activity against cervical cancer(;Yoshiki Furukawa等;Cell Reports Medicine;20231212;第04卷;第1-26页 * |
| iPSC-Derived neoantigen-specific CTL therapy for Ewing Sarcoma;Midori Ishii等;Cancer Immunol Res;第09卷;第1175-1186页 * |
| Selective deletion of human leukocyte antigens protects stem cell-derived islets from immune rejection;Audrey V. Parent等;Cell Reports;20210817;第36卷;第1-18页 * |
| Sustainable antiviral efficacy of rejuvenated HIV-specific cytotoxic T lymphocytes generated from induced pluripotent stem cells;Shoji Miki等;Journal of Virology;第96卷;都1-12页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115087732A (zh) | 2022-09-20 |
| JP7743980B2 (ja) | 2025-09-25 |
| CA3170361A1 (en) | 2021-08-12 |
| JPWO2021157685A1 (https=) | 2021-08-12 |
| EP4101924A4 (en) | 2024-03-13 |
| EP4101924A1 (en) | 2022-12-14 |
| US20230071538A1 (en) | 2023-03-09 |
| WO2021157685A1 (ja) | 2021-08-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP7440027B2 (ja) | 多能性幹細胞から免疫細胞療法用t細胞を誘導する方法 | |
| EP3580332B1 (en) | Methods and kits for generating mimetic innate immune cells from pluripotent stem cells | |
| JP6948072B2 (ja) | Cd8陽性t細胞を誘導する方法 | |
| JP6164746B2 (ja) | 抗原特異的t細胞の製造方法 | |
| JP2020010704A (ja) | 多能性幹細胞を用いた免疫機能再建法 | |
| EP3170896B1 (en) | Production method for pluripotent stem cells having antigen-specific t cell receptor gene | |
| WO2018143243A1 (ja) | 人工多能性幹細胞の作製方法 | |
| JPWO2016010155A1 (ja) | 抗原特異的t細胞受容体遺伝子を有する多能性幹細胞の製造方法 | |
| JP2023522326A (ja) | 条件付き不死化に関する制御可能な導入遺伝子を含む人工多能性細胞 | |
| CN115087732B (zh) | 来自人T细胞来源的iPS细胞的细胞毒性T细胞 | |
| JP7407415B2 (ja) | Cd4陽性制御性t細胞の製造方法 | |
| JP2024154083A (ja) | SARS-CoV-2特異的細胞傷害性Tリンパ球のT細胞受容体又はその機能的断片 | |
| JP7725014B2 (ja) | HTLV-1がコードするTax抗原特異的細胞傷害性Tリンパ球のT細胞受容体又はその機能的断片 | |
| EP4682165A1 (en) | T-cell receptor for hpv-specific cytotoxic t-cell, or functional fragment thereof | |
| US20220233665A1 (en) | Medicinal composition | |
| JP2025090024A (ja) | HTLV-1がコードするTax抗原特異的細胞傷害性Tリンパ球のT細胞受容体又はその機能的断片 | |
| WO2024248157A1 (ja) | 多能性幹細胞由来cd4陽性t細胞、その製造方法及びその用途 | |
| CN120303393A (zh) | 多能干细胞来源的t细胞群体及其祖细胞 | |
| CN120265300A (zh) | 来源于多能细胞的自然杀伤细胞谱系 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |