CN115068387A - Plant composition and application thereof in cosmetics - Google Patents
Plant composition and application thereof in cosmetics Download PDFInfo
- Publication number
- CN115068387A CN115068387A CN202210811828.XA CN202210811828A CN115068387A CN 115068387 A CN115068387 A CN 115068387A CN 202210811828 A CN202210811828 A CN 202210811828A CN 115068387 A CN115068387 A CN 115068387A
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- Prior art keywords
- truffle
- ginseng
- aging
- plant composition
- plant
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- 239000000203 mixture Substances 0.000 title claims abstract description 30
- 239000002537 cosmetic Substances 0.000 title claims abstract description 16
- 241000196324 Embryophyta Species 0.000 claims abstract description 26
- 235000008434 ginseng Nutrition 0.000 claims abstract description 22
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 21
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 21
- 241000609666 Tuber aestivum Species 0.000 claims abstract description 21
- 230000003712 anti-aging effect Effects 0.000 claims abstract description 18
- 240000008397 Ganoderma lucidum Species 0.000 claims abstract description 14
- 235000001637 Ganoderma lucidum Nutrition 0.000 claims abstract description 14
- 241000133134 Saussurea Species 0.000 claims abstract description 13
- 241000222336 Ganoderma Species 0.000 claims abstract description 8
- 241000208340 Araliaceae Species 0.000 claims abstract 7
- 238000000605 extraction Methods 0.000 claims description 18
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- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 8
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- IYRMWMYZSQPJKC-UHFFFAOYSA-N kaempferol Chemical compound C1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 IYRMWMYZSQPJKC-UHFFFAOYSA-N 0.000 description 2
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 2
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- FTVWIRXFELQLPI-ZDUSSCGKSA-N (S)-naringenin Chemical compound C1=CC(O)=CC=C1[C@H]1OC2=CC(O)=CC(O)=C2C(=O)C1 FTVWIRXFELQLPI-ZDUSSCGKSA-N 0.000 description 1
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- UBSCDKPKWHYZNX-UHFFFAOYSA-N Demethoxycapillarisin Natural products C1=CC(O)=CC=C1OC1=CC(=O)C2=C(O)C=C(O)C=C2O1 UBSCDKPKWHYZNX-UHFFFAOYSA-N 0.000 description 1
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
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- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 description 1
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- 235000005875 quercetin Nutrition 0.000 description 1
- 229960001285 quercetin Drugs 0.000 description 1
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- IZTQOLKUZKXIRV-YRVFCXMDSA-N sincalide Chemical compound C([C@@H](C(=O)N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(N)=O)NC(=O)[C@@H](N)CC(O)=O)C1=CC=C(OS(O)(=O)=O)C=C1 IZTQOLKUZKXIRV-YRVFCXMDSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/13—Coniferophyta (gymnosperms)
- A61K36/15—Pinaceae (Pine family), e.g. pine or cedar
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/285—Aucklandia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9755—Gymnosperms [Coniferophyta]
- A61K8/9767—Pinaceae [Pine family], e.g. pine or cedar
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/18—Antioxidants, e.g. antiradicals
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Epidemiology (AREA)
- Biotechnology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Birds (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Dermatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Toxicology (AREA)
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a plant composition and application thereof in cosmetics, and relates to the technical field of cosmetics. The composition comprises Ginseng radix, Ganoderma, herba Saussureae Involueratae and truffle. Through the synergistic effect of ginseng, ganoderma lucidum, saussurea involucrate and truffle, the collagen anti-aging cosmetic can effectively inhibit free radicals, inhibit glycosylation reaction and matrix metalloproteinase-1 (MMP-1) secretion, promote the generation of human type I collagen (COL-1), is safe to use, has no toxic or side effect, and can be well applied to anti-aging cosmetics.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to a plant composition and application thereof in cosmetics.
Background
Aging is an activity accompanying the development and development of life, and is a process of loss and degeneration of the body from constitutional materials, tissue structures to physiological functions. The aging process is continued from the fertilized egg to death, and the aging characteristics are only obviously shown until a certain stage. The physiological changes in the aging process of human body are mainly reflected in the loss of organism tissue cells and constitutional substances, the slowing of organism metabolic rate and the hypofunction of organism and organs.
At present, related anti-aging products are researched more and more aiming at the aging problem. The theory of free radicals is that the aging process of the organism is the result of the accumulation of free radicals continuously generated by the tissue cells of the organism, and the free radicals can cause DNA damage to cause mutation and induce tumor formation. Free radicals are intermediate products of normal metabolism, have strong reaction capability, and can oxidize various substances in cells to damage biological membranes. But also cross-link macromolecules such as proteins and nucleic acids, and influence the normal functions of the proteins and nucleic acids. Meanwhile, the theory of saccharification indicates that glucose is an important energy of cells, but it can randomly react with proteins and DNA to destroy them. This process, known as saccharification, can lead to several undesirable consequences. The enzyme subjected to the glycation is often out of function and cannot work normally, and the glycated protein is deformed and becomes unstable. As these processes are studied and well known, saccharification is considered as one of the causes of aging. Cross-linking occurs due to glycation, which allows chemical links to be formed between proteins and other macromolecular substances. The material that is crosslinked typically becomes stiffer, lacks elasticity, and is susceptible to tearing or breaking. In the aging body, crosslinking can lead to arteriosclerosis, wrinkles and joint stiffness.
In addition, research shows that MMP-1 (matrix metalloproteinase-1) is closely related to human skin aging, and one of the main reasons of skin aging is that the structure of the dermis layer is changed, so that the structure of the dermis layer of the skin is changed, and the skin dermis is changed in various ways, wherein due to the action of some external factors, Matrix Metalloproteinase (MMPs) in the body are activated, so that collagen and elastin which support the skin structure in the dermis of the skin are excessively degraded, and the skin has aging symptoms of shrinkage, inelasticity and the like. Based on the important role of MMP-1 in the aging process of skin, people gradually take inhibition of MMP-1 activity, inhibition of abnormal collagen degradation, reduction of skin inflammatory reaction, and prevention of wrinkles caused by skin photoaging as a new way to delay aging. Lim and the like find that flavonoid compounds such as quercetin, kaempferol, naringenin, apigenin, wogonin and the like have the effect of inhibiting the activity and expression of MPP-1 and can be used as main components of medicaments or cosmetics for preventing and treating skin inflammation and skin photoaging. In addition, the water extract of seeds of Cucurbita plant can inhibit the degradation and degradation of ECM protein (especially collagen), and inhibit ECM protease (especially MMPs), and can be used for relieving and preventing skin aging, reducing wrinkle and fine lines, increasing skin elasticity, smoothing and smoothing skin, and relieving inflammation.
Along with the increase of the demand of people on anti-aging products, the importance of safer and more natural anti-aging products is increasingly highlighted, and the anti-aging products of the existing plant composition usually have more components and uncertain curative effect, so that the plant composition with good anti-aging effect needs to be provided to meet the requirements of exact anti-aging effect, safety and no toxic or side effect.
Disclosure of Invention
The invention aims to provide a plant composition and an application thereof in cosmetics, the plant composition can effectively inhibit free radicals, inhibit secretion of matrix metalloproteinase-1 (MMP-1) and promote generation of human type I collagen (COL-1) through synergistic action of ginseng, ganoderma lucidum, saussurea involucrate and truffle, is safe to use, has no toxic or side effect, and can be well applied to anti-aging cosmetics.
In order to realize the purpose of the invention, the technical scheme of the invention is as follows:
in one aspect, the invention provides a plant composition comprising ginseng, ganoderma lucidum, saussurea involucrate and truffle.
Preferably, the mass ratio of the ginseng, the lucid ganoderma, the snow lotus and the truffle is 4: 1-4: 0.1-1: 0.1-1.
Further preferably, the mass ratio of the ginseng, the lucid ganoderma, the snow lotus and the truffle is 4: 2-4: 0.5-1: 0.5-1.
Most preferably, the mass ratio of the ginseng, the lucid ganoderma, the snow lotus and the truffle is 4:2:0.5: 0.5.
in another aspect, the present invention provides a method for extracting the plant composition, comprising the steps of:
mixing Ginseng radix, Ganoderma, herba Saussureae Involueratae, truffle and solvent, extracting, and separating to obtain filtrate.
Preferably, the extraction solvent is water and/or an alcohol solvent, more preferably at least one of water, methanol, ethanol, butanediol and isopropanol, more preferably a mixed solvent of water and butanediol, and more preferably a mixed solvent of water and butanediol, wherein the mass ratio of water to butanediol is 6-8: 2-4, most preferably the mass ratio of water to butanediol is 7: 3 in a solvent mixture.
Preferably, the ratio of the extracted feed to the extracted liquid is 1: 10 to 80(m/m), more preferably 1:20 (m/m).
Preferably, the extraction time is 1-3h, more preferably 2 h.
In a further aspect, the present invention provides a plant extract obtained according to the above extraction method.
In another aspect, the invention provides the application of the plant composition and/or the plant extract in preparing anti-aging cosmetics or anti-aging drugs and health care products.
Preferably, the anti-aging includes scavenging DPPH free radicals, inhibiting glycosylation reactions, inhibiting MMP-1 and collagen regeneration.
Finally, the present invention provides an anti-aging cosmetic comprising the above plant composition and/or plant extract.
The invention has the beneficial effects that:
the cosmetic raw materials prepared by the preparation method are safe and environment-friendly, have natural components, no toxic or side effect and definite anti-aging effect, and have excellent effects of resisting oxidation, inhibiting glycosylation reaction, inhibiting MMP-1 and stimulating collagen COL-1 secretion through experimental verification.
Detailed Description
The present invention will be further explained with reference to specific examples in order to make the technical means, the technical features, the technical objectives and the effects of the present invention easier to understand, but the following examples are only preferred embodiments of the present invention, and not all embodiments of the present invention. Based on the embodiments in the implementation, other embodiments obtained by those skilled in the art without any creative efforts belong to the protection scope of the present invention. In the following examples, unless otherwise specified, all the operations were performed by conventional methods, all the equipments were performed by conventional methods, and the materials of the equipments used in the respective examples were the same. All concentration percentages are mass concentrations unless otherwise specified. In the experiment of the invention, when the plant formula extraction filtrate is prepared into the solution with the specific concentration, all the solvents are 30 percent butanediol.
In the embodiment described below, it is preferred that,
the ginseng producing area Jilin Changbai mountain is Panax ginseng C.A. Meyer.
Ganoderma lucidum is produced in Shandong, and is Ganoderma lucdium (Curtis) P.Karst.
Sinkiang saussurea involucrata, and the variety is Saus-surea involucrata (Kar.et. Kir) Sch.
Yunnan of truffle production place, and the variety is Tuberer melanosporum.
Example 1
The weight ratio of ginseng, ganoderma lucidum, saussurea involucrate and truffle is as follows: 4:2:0.5:1.
Example 2
The weight ratio of ginseng, ganoderma lucidum, saussurea involucrate and truffle is as follows: 4:2:0.5:0.5.
Example 3
The weight ratio of ginseng, ganoderma lucidum, saussurea involucrate and truffle is as follows: 4:0:0.5:0.5.
Example 4
The weight ratio of ginseng, ganoderma lucidum, saussurea involucrate and truffle is as follows: 4:4:0.5:0.5.
Comparative example 1
The weight ratio of ginseng, ganoderma lucidum, saussurea involucrate and truffle is as follows: 4:2:0.5:0.
Comparative example 2
The weight ratio of ginseng, lucid ganoderma, snow lotus and truffle is as follows: 2:1:0:0.
Comparative example 3
The weight ratio of ginseng, ganoderma lucidum, saussurea involucrate and truffle is as follows: 0:0:1:1.
Extraction process
Weighing the medicinal materials according to the mixture ratio of the examples 1-4 and the comparative examples 1 and 2 respectively, and uniformly mixing the medicinal materials to obtain 5g of the mixture. Adding 70g of pure water and 30g of butanediol according to the material-liquid ratio of 1:20(m/m), uniformly stirring, heating in a water bath at 90 ℃, extracting for 2 hours, cooling the material liquid to 25 ℃, and performing suction filtration to obtain the plant formula extraction filtrate.
Result detection
1. DPPH free radical scavenging experiment
The experimental method comprises the following steps: the plant formula is taken to extract filtrate to prepare solution to be tested with the concentration of 5.0%, 10%, 15% and 20%. The DPPH solution is prepared by using absolute ethyl alcohol as a solvent for later use. Tube A: taking 1mL of solution to be detected and 1mL of 2X 10 -4 Uniformly mixing the mol/L DPPH solution; and (B) tube: 1mL of solvent and 1mL of 2X 10 -4 Uniformly mixing mol/L DPPH solution; c, pipe C: uniformly mixing 1mL of solvent with 1mL of solution to be detected; after each tube was left to react for 30min in the absence of light, the absorbance of A, B, C tubes was measured at 517 nm. The above experiment was carried out using the extraction filtrates obtained from the formulations of examples 1 to 4 and comparative examples 1 and 2, respectively,
DPPH free radical inhibition rate calculation formula: DPPH inhibition ratio (%) - (B + C-A)/B.
The results are as follows:
the DPPH free radical inhibition experiment result shows that the plant composition formula disclosed by the embodiment of the invention has a good free radical inhibition effect, the inhibition rate of the DPPH free radical reaches more than 50% under the concentration of 10%, 15% and 20%, and the effect is obvious. Among them, the radical inhibition effect of example 1 is the best, and 83.79% can be achieved at a concentration of 20%.
2. Anti-glycation assay
The experimental method comprises the following steps: adding deionized water into the plant formula extraction filtrate to prepare a 5% test solution, and then sequentially diluting the test solution into 2%, 1% and 0.5% test solutions in a gradient manner by using a PBS solution. Placing prepared sample groups (test solution + bovine serum albumin-fructose reaction solution), negative control groups (PBS solution + bovine serum albumin-fructose reaction solution) and blank control groups (test solution + PBS solution) with different concentrations in an incubator at 37 ℃ in a dark place for incubation, and detecting the fluorescence intensity of each group when the incubation is carried out for 5 days. And (3) spotting the mixed solution into a black 96-well plate, adjusting the excitation wavelength to 370nm and the emission wavelength to 440nm by using a microplate reader, and finally calculating the inhibition rate of the sample to be tested according to a formula.
The Inhibition Ratio (IR) calculation formula is as follows:
the experimental results are as follows:
the results of the 5% concentration test showed that the inhibition rates of glycation inhibition in vitro were substantially comparable, between 30% and 36%, with the exception of comparative example 1. In example 1, the inhibition rate of 19.2% is still achieved at a concentration of 0.5%.
3. Experiment for screening safety concentration of cytotoxic-CCK-8
The experimental method comprises the following steps: 96-well plates were incubated at 5% CO 2 After incubation in an incubator at 37 ℃ for 24h, the medium was aspirated, 100. mu.L of the plant formulation extraction filtrate (prepared at 3%, 5% concentrations, 3 duplicate wells each) was added, and 100. mu.L of serum-free medium solution (Bioagrio) was added to the blank. The control group was dimethyl sulfoxide. Adding 5% CO 2 Incubate at 37 ℃ for 24 h.
The culture was terminated after 24h, and 10. mu.L of CCK-8 was added to each well of the 96-well plate (care was taken to keep out of light). At 5% CO 2 After incubation in an incubator at 37 ℃ for 1h, the medium turned orange in color, at which time the OD was measured at a wavelength of 450nm using a microplate reader. The cell activity is in direct proportion to the OD value, and the sample safety is determined when the cell survival rate is more than 80 percentAnd (4) concentration.
Survival rate (sample OD 450/blank OD450) × 100%
The results are as follows:
the results show that the relative cell activity of the plant extract formula of the invention is more than 80 percent and reaches more than 100 percent under the concentration of 3 percent and 5 percent, and the plant extract formula has no cytotoxicity.
4. Anti-aging effect experiment
4.1 MMP-1 Activity detection experiment
The experimental method comprises the following steps: the plant composition extraction filtrate is prepared into two concentrations of 3% and 5%, and detection is carried out according to the operation instruction of MMP-1ELISA kit (BOSTER).
Experimental results for the formulation of example 1:
compared with the blank, UVB radiation enables the secretion amount of MMP-1 to be obviously increased, and the model establishment is successful. The extract obtained by the formula of the embodiment is added to reduce the secretion amount of MMP-1 of fibroblasts after UVB radiation, which shows that the formula of the invention has the function of inhibiting the secretion of MMP-1. Wherein KS5 showed a more pronounced inhibitory effect on MMP-1 secretion at 5% concentration.
4.2 COL-1 Activity detection experiment
The experimental method comprises the following steps: the plant formula extraction filtrate is prepared into 3% and 5% concentrations, and detection is carried out according to the operation instruction of a COL-1 kit (Shanghai enzyme-linked).
Experimental results for the formulation of example 1:
the result shows that the formulation of the embodiment of the invention can up-regulate the COL-1 secretion amount of the fibroblast after UVB radiation, which indicates that the formulation has the effect of promoting the COL-1 secretion. Wherein, the effect of promoting COL-1 secretion is more remarkable at the concentration of 3 percent.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. A plant composition is characterized by comprising ginseng, ganoderma lucidum, saussurea involucrate and truffle.
2. The plant composition as claimed in claim 1, wherein the mass ratio of ginseng, ganoderma lucidum, saussurea involucrate and truffle is 4: 1-4: 0.1-1: 0.1-1.
3. The plant composition as claimed in claim 2, wherein the mass ratio of ginseng, ganoderma lucidum, saussurea involucrate and truffle is 4: 2-4: 0.5-1: 0.5-1.
4. The plant composition as claimed in claim 3, wherein the mass ratio of ginseng, ganoderma lucidum, saussurea involucrate and truffle is 4:2:0.5: 0.5.
5. a method for extracting a plant composition according to any one of claims 1 to 4, comprising the steps of: mixing Ginseng radix, Ganoderma, herba Saussureae Involueratae, truffle and solvent, extracting, and separating to obtain filtrate.
6. The extraction method according to claim 5, wherein the extraction solvent is water and/or an alcohol solvent, and the ratio of the extracted material to the extracted liquid is 1: 10-80(m/m), and the extraction time is 1-3 h.
7. The extraction method according to claim 6, wherein the extraction solvent is a mixed solvent of water and butanediol, and the ratio of the extracted material to the liquid is 1:20(m/m), and the extraction time is 2 h.
8. Plant extracts obtained by the extraction process according to any one of claims 5 to 7.
9. Use of the plant composition according to any one of claims 1 to 4 and/or the plant extract according to claim 8 for the preparation of anti-aging cosmetic or anti-aging pharmaceutical, nutraceutical products.
10. An anti-aging cosmetic comprising the plant composition according to any one of claims 1 to 4 and/or the plant extract according to claim 8.
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| WO2011140856A1 (en) * | 2010-05-13 | 2011-11-17 | 上海家化联合股份有限公司 | Snow lotus extract, preparing method and application thereof |
| CN110613672A (en) * | 2019-11-05 | 2019-12-27 | 湖北创界生物科技有限公司 | Face-refreshing and skin-brightening mask composition, mask and preparation method |
| CN112675098A (en) * | 2021-02-02 | 2021-04-20 | 广东梵蜜琳生物科技有限公司 | Smoothing toner containing composition for regulating time rhythm and preparation method thereof |
| CN113288831A (en) * | 2021-06-11 | 2021-08-24 | 北京工商大学 | Application of ganoderma lucidum and/or ginseng in preparation of anti-inflammatory factor product and skin care product or cosmetic thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011140856A1 (en) * | 2010-05-13 | 2011-11-17 | 上海家化联合股份有限公司 | Snow lotus extract, preparing method and application thereof |
| CN110613672A (en) * | 2019-11-05 | 2019-12-27 | 湖北创界生物科技有限公司 | Face-refreshing and skin-brightening mask composition, mask and preparation method |
| CN112675098A (en) * | 2021-02-02 | 2021-04-20 | 广东梵蜜琳生物科技有限公司 | Smoothing toner containing composition for regulating time rhythm and preparation method thereof |
| CN113288831A (en) * | 2021-06-11 | 2021-08-24 | 北京工商大学 | Application of ganoderma lucidum and/or ginseng in preparation of anti-inflammatory factor product and skin care product or cosmetic thereof |
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