CN115067441B - Lepidoptera noctuid larva feed for parasitic wasp propagation and preparation method thereof - Google Patents

Lepidoptera noctuid larva feed for parasitic wasp propagation and preparation method thereof Download PDF

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CN115067441B
CN115067441B CN202210672506.1A CN202210672506A CN115067441B CN 115067441 B CN115067441 B CN 115067441B CN 202210672506 A CN202210672506 A CN 202210672506A CN 115067441 B CN115067441 B CN 115067441B
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lepidoptera
powder
feed
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larva
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CN115067441A (en
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王蓓蓓
曹春霞
黄大野
郑娇莉
朱志刚
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Hubei Biopesticide Engineering Research Center
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Hubei Biopesticide Engineering Research Center
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/90Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/26Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/158Fatty acids; Fats; Products containing oils or fats
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The invention relates to a lepidoptera nocturnal moth larva feed for parasitic wasp propagation and a preparation method thereof, wherein the feed comprises the following raw materials in parts by weight: soybean powder, barley flour, yeast powder, glycerol, honey, pea seedling powder, lepidoptera larva feces, chlorella powder, apple peel pulp, grape peel pulp, propionic acid, agar and sterile water. The feed disclosed by the invention utilizes waste materials, saves cost, and in addition, the raw materials are synergistic, the parasitic rate of parasitic bees is increased, the raw materials are simple, the content of chemical reagents is low, the hardness is moderate, the feed has obvious fragrance, and experiments show that the feed disclosed by the invention can be used for insect larvae of multiple noctuidae such as spodoptera frugiperda, prodenia litura, asparagus caterpillar, cotton bollworm and the like and in the parasitic process of parasitic bees, and the physiological state of the fed larvae is stable.

Description

Lepidoptera noctuid larva feed for parasitic wasp propagation and preparation method thereof
Technical Field
The invention belongs to the technical field of insect artificial feed, and particularly relates to a lepidoptera noctuid larva feed for parasitic wasp propagation and a preparation method thereof.
Background
Lepidoptera (Lepidoptera) nocturnal moth (nocuitae) insects are widely distributed in various countries around the world, are mostly phytophagous, harm various crops, and have great influence on agricultural production. Among them, spodoptera frugiperda, prodenia litura, cotton bollworm, asparagus caterpillar, etc. are important pests of crops in China, the main control means at present is chemical control, but long-term use of chemical pesticides makes the agricultural product pesticide residue exceed standard, the pest resistance is enhanced, and the ecological balance is destroyed. Natural enemies in biological control means are a green and safe pest control means. Parasitic wasps are an important biological control resource as natural enemies of pests, can be used as biological pesticide products after mass propagation, and have great application value in green control of the pests.
The parasitic wasps lay eggs in the host and utilize the host to grow until the offspring parasitic wasps emerge and ultimately cause death of the host. The parasitic wasp is dependent on the host, and in the process of the parasitic host, the parasitic wasp can select host pests with good states, and has higher requirements on the insect age, smell, environmental temperature and humidity and the like of the host. The existing lepidoptera insect larva feed contains various chemical reagents, and a host and a feeding box which take the feed have residual smell of the feed, so that parasitic bees are averted, the search and the parasitism of the parasitic bees on the host are affected, and the parasitism rate and the emergence rate of the parasitic bees are reduced. The method separates a large number of low-age host pests from feed, has extremely large workload, is easy to cause damage and even death of the host pests, greatly improves the working cost, affects the parasitic efficiency, is difficult to obtain a large number of parasitic bees, and affects the propagation of the parasitic bees. Moreover, if the proportion of water and agar in the feed is improper, the feed is hard or moist, the feeding of the host larvae is affected, and the temperature and humidity in the feeding box are changed, so that the death rate of the host larvae is increased. Therefore, the feed for raising the host and the feed with moderate hardness, in which the parasitic wasps can coexist in the environment, are key elements for the propagation of the parasitic wasps.
The artificial feed for lepidoptera nocturnal insects can solve the seasonal shortage of the natural condition of feeding insects by using plants, can ensure that the development age of host larvae is consistent, has stable physiological state and is convenient for the parasitic and management adjustment of parasitic bees, so that the artificial large-scale feeding of host pests is an important experimental material for the development of biological pest control products, and can feed an important basis for the basic research of entomology and the control of agricultural pests.
At present, various feed formulas exist, the nutrition components of the used feed are similar and complex in variety, and the additives are more, but the proportion is different, so that various preservative components are commonly added for preserving and improving the disease resistance of insects. Parasitic wasps are very sensitive to antibiotics and preservatives, and after the host takes the feed containing the antibiotics and the preservatives, the parasitic wasps have evasion effect, and the parasitic wasps have reduced parasitic rate and emergence rate.
The present invention has been made in view of the above-mentioned circumstances.
Disclosure of Invention
In order to solve the problems of low larva eclosion rate, low parasitic wasp parasitic rate and the like caused by more chemical additives in feeds for lepidoptera nocturnal larvae in the prior art, the invention provides the lepidoptera nocturnal larva feed for expanding propagation of parasitic wasps and the preparation method thereof.
The invention provides a lepidoptera nocturnal larva feed for parasitic wasp propagation, which is prepared from the following raw materials in parts by weight: 45-75 parts of soybean powder, 30-50 parts of barley flour, 15-25 parts of yeast powder, 18.9-31.5 parts of glycerol, 5-10 parts of honey, 2-10 parts of pea seedling powder, 2-5 parts of lepidoptera larva feces, 1-3 parts of chlorella powder, 2-6 parts of apple peel pulp, 2-5 parts of grape peel pulp, 0.0693-0.1485 parts of propionic acid, 6-8 parts of agar and 200-400 parts of sterile water.
Further, the feed comprises the following raw materials in parts by weight: 55-65 parts of soybean powder, 35-45 parts of barley flour, 17-23 parts of yeast powder, 16.38-28.98 parts of glycerol, 6-8 parts of honey, 1-5 parts of pea seedling powder, 3-4 parts of lepidoptera larva feces, 1.5-2 parts of chlorella powder, 4-5 parts of apple peel pulp, 3-4 parts of grape peel pulp, 0.0792-0.1386 parts of propionic acid, 6.5-7.5 parts of agar and 220-360 parts of sterile water.
The pea seedling powder provided by the invention is rich in cellulose and vitamins, and the lepidoptera larva feces contains nitrogen, phosphorus, potassium, trace elements and proteins, so that the chlorella can provide high-quality proteins, vitamins and the like, and the apple peel pulp and grape peel pulp contain peel vitamins and anthocyanin, so that the pea seedling powder has good oxidation resistance and flavor. The lepidoptera larva feces, apple peel pulp and grape peel pulp are used as waste materials for recycling, so that the cost is saved.
The glycerol plays a role in thickening, preventing feed from cracking, the honey plays a role in attracting parasitic wasps, and the glycerol contains enzymes and nutrients, the propionic acid plays a role in preserving, and the agar has a solidification effect, is easy to block and is beneficial to cutting.
The sterile water is ultrapure water or pure water.
Further, the soybean meal is prepared by the following method: cleaning semen glycines with water at temperature higher than 80deg.C, soaking for 8-12min, baking in oven at 120-130deg.C for 12-14 hr, and grinding into powder to obtain the final product.
The soybean powder prepared by the invention is not easy to mildew, is not easy to paste after baking in an oven, has uniform texture and is fine and smooth.
Further, the pea seedling powder is prepared by the following method: soaking peas in water for 20-28h, performing water planting, growing for one week, taking and cleaning the seedlings, baking in a baking oven at 120-130 ℃ for 12-24h, and grinding into powder to obtain the pea seedling powder.
Further, the lepidoptera larva feces is prepared by the following method: and (3) placing the feces generated after the spodoptera litura and/or the spodoptera exigua ingests the cabbages in a baking oven at 120-130 ℃ for 12-24 hours, and grinding the feces into powder to obtain the lepidoptera larva feces.
When the feces are selected from the feces produced by spodoptera litura and/or spodoptera exigua, the two may be in any ratio.
Further, the apple peel pulp or grape peel pulp is prepared by cleaning apple peel or grape peel, grinding and pulping.
The second object of the invention is to provide a preparation method of the lepidoptera nocturnal moth larva feed for parasitic wasp propagation, which comprises the following steps:
(1) Weighing the raw materials for standby according to the weight parts of the raw materials;
(2) Uniformly mixing the standby soybean powder, barley flour, yeast powder, pea seedling powder, lepidoptera larva feces and chlorella powder to obtain a mixture A;
(3) Uniformly mixing glycerol, honey, propionic acid, apple peel pulp, grape peel pulp and a part of water to obtain a mixture B;
(4) Uniformly mixing the mixture A and the mixture B to obtain a mixture C;
(5) Adding the rest water into the agar, heating, cooling, pouring into the mixture C, stirring uniformly, cutting into small pieces, and refrigerating in a refrigerator to obtain the lepidoptera nocturnal moth larva feed for parasitic wasp propagation.
Further, the mass ratio of the water in the step (3) to the water in the step (5) is 3:1-4:1.
Further, the heating temperature in the step (5) is 95-100 ℃.
Compared with the prior art, the invention has the beneficial effects that:
(1) According to the feed disclosed by the invention, waste materials are utilized, so that the cost is saved, in addition, the raw materials are synergistic, the parasitic rate of parasitic bees is increased, the raw materials are simple, the content of chemical reagents is low, the hardness is moderate, the feed has obvious fragrance, and experiments show that the feed disclosed by the invention can be used for insect larvae of various noctuidae such as spodoptera frugiperda, prodenia litura, asparagus caterpillar, cotton bollworm and the like and in the parasitic process of the parasitic bees, and the physiological state of the fed larvae is stable;
(2) The feed preparation method is simple, does not use special equipment, has strong operability and low production cost, and can be used for mass production.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, based on the examples herein, which are within the scope of the invention as defined by the claims, will be within the scope of the invention as defined by the claims.
Example 1
The lepidoptera nocturnal moth larva feed for parasitic wasp propagation in the embodiment is prepared from the following raw materials: 45g of soybean meal, 50g of barley meal, 15g of yeast powder, 25ml of glycerol, 5g of honey, 10g of pea seedling powder, 2g of lepidoptera larva feces, 3g of chlorella powder, 2g of apple peel pulp, 5g of grape peel pulp, 70 μl of propionic acid, 8g of agar and 200g of sterile water.
The soybean powder is prepared by the following steps: cleaning semen glycines with water above 80deg.C, soaking for 8min, baking in oven at 120deg.C for 14 hr, and grinding into powder to obtain the soybean powder.
The pea seedling powder is prepared by the following steps: soaking peas in water for 20h, carrying out water planting, taking and cleaning the seedlings after growing for one week, baking in a baking oven at 120 ℃ for 24h, and grinding into powder to obtain the pea seedling powder.
The lepidoptera larva feces is prepared by the following method: and (3) placing the feces generated after the cabbage is eaten by the prodenia litura in a baking oven at 120 ℃ for baking for 24 hours, and grinding the feces into powder to obtain the lepidoptera larva feces.
The apple peel pulp or grape peel pulp is prepared by cleaning apple peel or grape peel, grinding and pulping.
The preparation method of the feed comprises the following steps:
(1) Weighing the raw materials for standby according to the weight parts of the raw materials;
(2) Uniformly mixing the standby soybean powder, barley flour, yeast powder, pea seedling powder, lepidoptera larva feces and chlorella powder to obtain a mixture A;
(3) Uniformly mixing glycerol, honey, propionic acid, apple peel pulp, grape peel pulp and a part of water to obtain a mixture B;
(4) Uniformly mixing the mixture A and the mixture B to obtain a mixture C;
(5) Adding the rest water into the agar, heating to 95 ℃, cooling, pouring into the mixture C, stirring uniformly, cutting into small pieces, and putting into a refrigerator for refrigeration to obtain the lepidoptera nocturnal larva feed for parasitic wasp propagation;
wherein the mass ratio of the water in the step (3) to the water in the step (5) is 3:1.
Example 2
The lepidoptera nocturnal moth larva feed for parasitic wasp propagation in the embodiment is prepared from the following raw materials: 55g of soybean meal, 35g of barley meal, 23g of yeast powder, 13ml of glycerol, 8g of honey, 1g of pea seedling powder, 4g of lepidoptera larva feces, 1.5g of chlorella powder, 5g of apple peel pulp, 3g of grape peel pulp, 140 mu l of propionic acid, 6.5g of agar and 360g of sterile water.
The soybean powder is prepared by the following steps: cleaning semen glycines with water at 80deg.C, soaking for 10min, baking in oven at 125deg.C for 13 hr, and grinding into powder to obtain the soybean powder.
The pea seedling powder is prepared by the following steps: soaking peas in water for 24 hours, carrying out water planting, taking and cleaning the seedlings after growing for one week, baking the seedlings in a baking oven at 125 ℃ for 18 hours, and grinding the seedlings into powder to obtain the pea seedling powder.
The lepidoptera larva feces is prepared by the following method: and (3) placing the feces generated after the asparagus caterpillar feeds the cabbage into a baking oven at 125 ℃ for baking for 18 hours, and grinding the feces into powder to obtain the lepidoptera larva feces.
The apple peel pulp or grape peel pulp is prepared by cleaning apple peel or grape peel, grinding and pulping.
The preparation method of the feed comprises the following steps:
(1) Weighing the raw materials for standby according to the weight parts of the raw materials;
(2) Uniformly mixing the standby soybean powder, barley flour, yeast powder, pea seedling powder, lepidoptera larva feces and chlorella powder to obtain a mixture A;
(3) Uniformly mixing glycerol, honey, propionic acid, apple peel pulp, grape peel pulp and a part of water to obtain a mixture B;
(4) Uniformly mixing the mixture A and the mixture B to obtain a mixture C;
(5) Adding the rest water into the agar, heating to 98 ℃, cooling, pouring into the mixture C, uniformly stirring, cutting into small pieces, and putting into a refrigerator for refrigeration to obtain the lepidoptera nocturnal larva feed for parasitic wasp propagation;
wherein the mass ratio of the water in the step (3) to the water in the step (5) is 3.5:1.
Example 3
The lepidoptera nocturnal moth larva feed for parasitic wasp propagation in the embodiment is prepared from the following raw materials: 60g of soybean powder, 40g of barley flour, 20g of yeast powder, 20ml of glycerin, 7.5g of honey, 6g of pea seedling powder, 3.5g of lepidoptera larva feces, 2g of chlorella powder, 4g of apple peel pulp, 3.5g of grape peel pulp, 110 mu l of propionic acid, 7g of agar and 300g of sterile water.
The soybean powder is prepared by the following steps: cleaning semen glycines with water above 80deg.C, soaking for 12min, baking in oven at 130deg.C for 12 hr, and grinding into powder to obtain the soybean powder.
The pea seedling powder is prepared by the following steps: soaking peas in water for 28h, carrying out water planting, taking and cleaning the seedlings after growing for one week, baking in a baking oven at 130 ℃ for 12h, and grinding into powder to obtain the pea seedling powder.
The lepidoptera larva feces is prepared by the following method: and (3) placing the feces generated after the spodoptera litura and the spodoptera exigua eat the cabbage in a baking oven at 130 ℃ for 12 hours, and grinding the feces into powder to obtain the lepidoptera larva feces, wherein the mass ratio of the spodoptera exigua to the spodoptera exigua is 1:1.
The apple peel pulp or grape peel pulp is prepared by cleaning apple peel or grape peel, grinding and pulping.
The preparation method of the feed comprises the following steps:
(1) Weighing the raw materials for standby according to the weight parts of the raw materials;
(2) Uniformly mixing the standby soybean powder, barley flour, yeast powder, pea seedling powder, lepidoptera larva feces and chlorella powder to obtain a mixture A;
(3) Uniformly mixing glycerol, honey, propionic acid, apple peel pulp, grape peel pulp and a part of water to obtain a mixture B;
(4) Uniformly mixing the mixture A and the mixture B to obtain a mixture C;
(5) Adding the rest water into the agar, heating to 100 ℃, cooling, pouring into the mixture C, uniformly stirring, cutting into small pieces, and putting into a refrigerator for refrigeration to obtain the lepidoptera nocturnal larva feed for parasitic wasp propagation;
wherein the mass ratio of the water in the step (3) to the water in the step (5) is 4:1.
Example 4
The lepidoptera nocturnal moth larva feed for parasitic wasp propagation in the embodiment is prepared from the following raw materials: 65g of soybean powder, 35g of barley flour, 23g of yeast powder, 13ml of glycerol, 8g of honey, 1g of pea seedling powder, 4g of lepidoptera larva feces, 1.5g of chlorella powder, 5g of apple peel pulp, 3g of grape peel pulp, 140 mu l of propionic acid, 6.5g of agar and 220g of sterile water.
The soybean powder is prepared by the following steps: cleaning semen glycines with water at 80deg.C, soaking for 9min, baking in oven at 123 deg.C for 12 hr, and grinding into powder to obtain the soybean powder.
The pea seedling powder is prepared by the following steps: soaking peas in water for 22h, carrying out water planting, taking and cleaning the seedlings after growing for one week, baking in a baking oven at 123 ℃ for 20h, and grinding into powder to obtain the pea seedling powder.
The lepidoptera larva feces is prepared by the following method: and (3) placing the feces generated after the cabbage is eaten by the prodenia litura in a baking oven at 123 ℃ for baking for 20 hours, and grinding the feces into powder to obtain the lepidoptera larva feces.
The apple peel pulp or grape peel pulp is prepared by cleaning apple peel or grape peel, grinding and pulping.
The feed preparation method of this example is the same as that of example 1.
Example 5
The lepidoptera nocturnal moth larva feed for parasitic wasp propagation in the embodiment is prepared from the following raw materials: 75g of soybean meal, 30g of barley meal, 25g of yeast powder, 15ml of glycerol, 10g of honey, 2g of pea seedling powder, 5g of lepidoptera larva feces, 1g of chlorella powder, 6g of apple peel pulp, 2g of grape peel pulp, 150 μl of propionic acid, 6g of agar and 400g of sterile water.
The soybean powder is prepared by the following steps: cleaning semen glycines with water above 80deg.C, soaking for 11min, baking in oven at 128 deg.C for 13 hr, and grinding into powder to obtain the soybean powder.
The pea seedling powder is prepared by the following steps: soaking peas in water for 26 hours, carrying out water planting, taking and cleaning the seedlings after growing for one week, baking in a baking oven at 128 ℃ for 15 hours, and grinding into powder to obtain the pea seedling powder.
The lepidoptera larva feces is prepared by the following method: and (3) placing the feces generated after the cabbage is eaten by the prodenia litura in a baking oven at 128 ℃ for 15 hours, and grinding the feces into powder to obtain the lepidoptera larva feces.
The apple peel pulp or grape peel pulp is prepared by cleaning apple peel or grape peel, grinding and pulping.
The feed preparation method of this example is the same as that of example 2.
Comparative example 1
Feed was prepared according to the formulation and method of example 1 in patent CN 103947874B.
Comparative example 2
Feed was prepared according to the formulation and method of example 2 in patent CN 1151729C.
Comparative example 3
The feed material and the preparation method of this comparative example were the same as in example 3, except that no lepidopteran larva feces was added to the material.
Comparative example 4
The feed material and the preparation method of this comparative example were the same as in example 3, except that chlorella powder was not added to the material.
Comparative example 5
The feed material and the preparation method of this comparative example were the same as in example 3, except that no pea seedling powder was added to the material.
Comparative example 6
The feed material and the preparation method of this comparative example were the same as in example 3, except that apple peel pulp and grape peel pulp were not added to the material.
Test example 1
The feeds prepared in examples 1 to 5 and comparative examples 1 to 6 were used for feeding the newly hatched larvae of spodoptera frugiperda, respectively, and each group of feeds was fed 300 heads (each 100 heads is an experimental repeated group, 3 groups are total), the final pupation rate and the weight of the pupae were weighed, and the eclosion rate of these pupae was counted, and the results are shown in table 1.
TABLE 1
As is clear from Table 1, the feeds prepared in examples 1 to 5 were fed to Spodoptera frugiperda, and the difference in pupation rate, pupation weight and emergence rate of larvae was small in the range of the ratio. The feed formulas of the comparative example 1 and the comparative example 2 are single in nutrition and more in chemical preservative, and compared with the feed formulas of the examples 1-5, the pupation rate, the pupation weight and the eclosion rate of the larvae are obviously reduced; compared with the embodiment 1-5, the embodiment 3 and the embodiment 4 have the advantages that the pupa weight is reduced, the embodiment 3 is not added with lepidoptera larva feces, the larva feces contains rich nitrogen, phosphorus, trace elements and crude proteins, the larva feces are clean, the larva feces are used as feed to improve the nutrition condition of insects, the pupa weight is increased, the embodiment 4 is not added with chlorella powder, and the chlorella powder contains a large amount of high-quality proteins and vitamins and can enhance the nutrition and immunity of the larva; compared with the embodiment 1-5, the pupation rate and the pupation weight of the larvae are reduced in the comparative example 5, no pea seedling powder is added in the comparative example 5, and the pea seedling powder contains various B vitamins and dietary fibers, so that the larvae can be supplied with nutrition and can digest the feed conveniently; comparative example 6 has a reduced feathering rate compared with examples 1 to 5, and no apple peel pulp and no grape peel pulp are added in comparative example 6, and the two peel contains various alcohols and flavonoids, so that the effects of resisting oxidation and enhancing resistance are achieved.
Test example 2
The feeds prepared in examples 1 to 5 and comparative examples 1 to 6 were used for feeding the newly hatched larvae of asparagus caterpillar, respectively, 300 feeds were fed per group (each 100 feeds is an experimental repeated group, 3 groups are total), the final pupation rate and the weight of the pupae were weighed, and the eclosion rate of the pupae was counted, and the results are shown in Table 2.
TABLE 2
As is clear from Table 2, the feeds prepared in examples 1 to 5 were fed to spodoptera exigua, and the difference in pupation rate, pupation weight and emergence rate of larvae was small in the range of the ratio. The larva pupation rate, the pupation weight and the emergence rate of the comparative examples 1 and 2 are obviously reduced compared with the examples 1-5; comparative example 3 and comparative example 4 have reduced pupa weight compared to examples 1-5; comparative example 5 has a reduced rate of larval pupation compared to examples 1-5; comparative example 6 has a reduced feathering rate compared to examples 1-5.
Test example 3
The feeds prepared in examples 1 to 5 and comparative examples 1 to 6 were used for feeding the newly hatched larvae of asparagus caterpillar to the second age, 300 heads (each 100 heads is an experimental repeated group, 3 groups total) of the second-age larvae were taken, parasitic wasps were used, and the parasitic wasps were counted for parasitic rate and emergence rate, and the results are shown in table 3.
TABLE 3 Table 3
As is clear from Table 3, the feed prepared in examples 1 to 5 was used for parasitic by using the Menia crassipes after feeding beet armyworms, and the parasitic ratio were not greatly different in the range of the ratio change. Since comparative examples 1 and 2 have a large amount of chemical components and emit smell, the search and parasitism of parasitic wasps on hosts are seriously affected, and the parasitic ratio and the parasitic wasp emergence rate of comparative examples 1 and 2 are remarkably reduced compared with examples 1 to 5; the parasitic wasp emergence rate of comparative example 3, comparative example 4 and comparative example 5 is reduced compared with examples 1-5, the components lacking in comparative examples 3, 4 and 5 make the host lack high-quality proteins, microelements, dietary fibers and the like, the physiological properties of the host are more general, after parasitic wasps are parasitic, the host does not eat any more gradually, parasitic wasp eggs survive by virtue of original nutrients in the host, the physiological state of the parasitic wasp is related to the state of the host, and therefore the emergence rate is reduced; the parasitic wasp emergence rate of comparative example 6 was slightly reduced as compared with examples 1 to 5, because the aromatic smell emitted from the pericarp attracts parasitic wasp parasitism, and it is rich in various vitamins and the like, improving emergence rate.
The foregoing is merely illustrative of the present invention, and the present invention is not limited thereto, and any person skilled in the art will readily recognize that variations or substitutions are within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims (8)

1. The lepidoptera nocturnal moth larva feed for parasitic wasp propagation is characterized by being prepared from the following raw materials in parts by weight: 45-75 parts of soybean powder, 30-50 parts of barley flour, 15-25 parts of yeast powder, 18.9-31.5 parts of glycerol, 5-10 parts of honey, 2-10 parts of pea seedling powder, 2-5 parts of lepidoptera larva feces, 1-3 parts of chlorella powder, 2-6 parts of apple peel pulp, 2-5 parts of grape peel pulp, 0.0693-0.1485 parts of propionic acid, 6-8 parts of agar and 200-400 parts of sterile water.
2. The feed for lepidoptera nocturnal larva for parasitic wasp propagation according to claim 1, wherein the soybean meal is prepared by the following method: cleaning semen glycines with water at temperature higher than 80deg.C, soaking for 8-12min, baking in oven at 120-130deg.C for 12-14 hr, and grinding into powder to obtain the final product.
3. The feed for the propagation of parasitic wasps to form lepidoptera nocturnal larvae according to claim 1, wherein the pea seedling powder is prepared by the following method: soaking peas in water for 20-28h, performing water planting, growing for one week, taking and cleaning the seedlings, baking in a baking oven at 120-130 ℃ for 12-24h, and grinding into powder to obtain the pea seedling powder.
4. The lepidoptera nocturnal larva feed for parasitic wasp propagation according to claim 1, wherein the lepidoptera larva feces is prepared by the following method: and (3) placing the feces generated after the spodoptera litura and/or the spodoptera exigua ingests the cabbages in a baking oven at 120-130 ℃ for 12-24 hours, and grinding the feces into powder to obtain the lepidoptera larva feces.
5. The feed for expanded propagation of parasitic wasps of the lepidoptera nocturnal moth family larva according to claim 1, wherein the apple peel pulp or grape peel pulp is prepared by washing, grinding and pulping apple peel or grape peel.
6. A method of preparing a feed for lepidoptera nocturnal larvae of the family lepidoptera for propagation of parasitic wasps as claimed in any one of claims 1 to 5, comprising the steps of:
(1) Weighing the raw materials for standby according to the weight parts of the raw materials;
(2) Uniformly mixing the standby soybean powder, barley flour, yeast powder, pea seedling powder, lepidoptera larva feces and chlorella powder to obtain a mixture A;
(3) Uniformly mixing glycerol, honey, propionic acid, apple peel pulp, grape peel pulp and a part of water to obtain a mixture B;
(4) Uniformly mixing the mixture A and the mixture B to obtain a mixture C;
(5) Adding the rest water into the agar, heating, cooling, pouring into the mixture C, stirring uniformly, cutting into small pieces, and refrigerating in a refrigerator to obtain the lepidoptera nocturnal moth larva feed for parasitic wasp propagation.
7. The method for preparing a feed for lepidoptera nocturnal larvae for parasitic wasp propagation according to claim 6, wherein the mass ratio of water in the step (3) to water in the step (5) is 3:1-4:1.
8. The method for preparing a feed for lepidoptera nocturnal larva as claimed in claim 6, wherein the heating temperature in the step (5) is 95-100 ℃.
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