CN115067333A - Application of diethyl aminoethyl hexanoate in cotton planting and initiator - Google Patents
Application of diethyl aminoethyl hexanoate in cotton planting and initiator Download PDFInfo
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- A01N37/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
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Abstract
The invention discloses application of diethyl aminoethyl hexanoate in cotton planting and an initiator thereof, wherein the diethyl aminoethyl hexanoate is a novel plant regulator, is easy to prepare, convenient to operate and nontoxic. Diethyl aminoethyl hexanoate can regulate the growth of crops. According to the invention, through simple initiation treatment of the diethyl aminoethyl hexanoate seeds, the leaf area of cotton seedlings can be increased, the relative chlorophyll content is increased, and the accumulation of cotton biomass and the seedling strengthening are promoted. The stem thickness and the seedling height are improved; the number of the air holes is increased, thereby being beneficial to promoting photosynthesis and transpiration and promoting root development, thereby enhancing the competition and growth capacity of the cotton in the seedling stage and simultaneously improving the effect of germination and seedling emergence of the seeds. The increase of the leaf area is beneficial to quickly occupying space, preventing weeds from growing to a certain extent, increasing the accumulation of biomass to meet the requirement of later growth, and improving germination and emergence can relieve the problem of difficult full-seedling sowing.
Description
Technical Field
The invention relates to the field of cotton planting and production, in particular to application of diethyl aminoethyl hexanoate in cotton planting and an initiator.
Background
In cotton planting in China, particularly in Yangtze river basin, the late direct seeding mode is popularized at present, two-season crops can be planted in the late direct seeding mode, the economic benefit is high, but the mode has some technical problems which are difficult to solve, the climate condition is unstable along with the delay of the seeding period, the early growth of cotton is influenced, in the production of cotton, the leaf area of the cotton at the seedling stage is small, the open space cannot be occupied quickly, weeds near the cotton seedlings are easy to grow, nutrients are easy to compete, and the growth and development of cotton are influenced. Therefore, increasing the rapid growth of cotton at the seedling stage and increasing leaf area can ameliorate this problem. In addition, the biomass accumulation in the seedling stage is less, the requirement of later-stage reproductive organ development cannot be met, the problem of difficulty in one-time whole seedling sowing in a cotton direct sowing mode still exists, and seedling shortage and ridge breaking can be caused in serious cases. Therefore, how to increase the leaf area of cotton seedlings, improve the land occupation space quickly caused by biomass accumulation and prevent the growth of weeds; how to broadcast the whole seedling at one time is a technical problem which needs to be solved urgently. The suitable reagent seed soaking treatment is an effective way.
In the prior art, the emergence rate is increased and strong seedlings are cultivated by soaking seeds in warm water or dressing seeds with medicament, but the problems of increasing the leaf area, promoting photosynthesis and improving biomass accumulation cannot be solved, and the demand of a late direct seeding mode cannot be met. In addition, the seed dressing with the pesticide has the defects of complex seed dressing components, poor control of farmers, adverse effect caused by wrong seed dressing components and concentration allocation and higher cost. After seed dressing, the seed is not suitable for seed soaking or germination acceleration.
At present, the following problems still exist in the cotton production and planting process:
1) the leaf area is small in the seedling stage, the empty land cannot be quickly occupied, and weeds grow in clusters.
2) The seedling emergence is slow, the seedling can not quickly emerge in proper seedling emergence time, and the whole seedling is difficult to be sown at one time.
3) The cotton seedlings grow slowly, the early accumulation effect of the vegetative organs is weak, and the development requirement of later reproductive organs cannot be met.
4) Cotton seedlings are weak in photosynthesis and cannot meet the requirement for the capacity of accumulating energy substances.
5) There is no simple seed soaking agent and use method which are suitable for farmers to directly prepare themselves.
Researches show that suitable reagent seed soaking can increase leaf area and biomass accumulation, regulate and control cotton growth, improve plant emergence rate, improve seedling survival rate, promote individual growth and development, enhance stress resistance and improve yield, so that the method is a technology with good application prospect in cotton agricultural production. However, no seed soaking method and material really suitable for cotton, especially for cotton late-sowing planting are available.
Therefore, there is a need for a seed soaking material and method suitable for cotton planting.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides an application of diethyl aminoethyl hexanoate in cotton planting and an initiator.
In order to achieve the purpose, the invention designs application of diethyl aminoethyl hexanoate in cotton planting.
The diethyl aminoethyl hexanoate (DA-6, chemical formula C) 12 H 25 NO 2 ) Is a novel plant regulator, is easy to prepare, convenient to operate and nontoxic. The diethyl aminoethyl hexanoate has the function of regulating and controlling the growth of crops.
The invention also provides application of diethyl aminoethyl hexanoate in increasing leaf area, improving germination rate and emergence rate and improving photosynthesis to promote biomass accumulation in the cotton planting process.
The invention also provides a seed initiator for increasing the leaf area of cotton in seedling stage, improving the germination speed and the rate of emergence and improving the photosynthesis to promote the biomass accumulation, wherein the seed initiator comprises diethyl aminoethyl hexanoate.
Furthermore, in the seed initiator, the content of the diethyl aminoethyl hexanoate is 20-160 mg/ml.
Still further, the content of the diethyl aminoethyl hexanoate is 40 mg/L.
According to actual conditions, the seed initiator can be prepared into mother liquor for storage, and then diluted to the concentration for use when in use.
The invention also provides a method for increasing the leaf area of cotton seedlings, improving the germination speed and the rate of emergence, and improving photosynthesis to promote biomass accumulation, which comprises the following steps:
1) uniformly tedding the pretreated cotton seeds, then soaking the cotton seeds in a sodium hypochlorite solution, washing and airing;
2) seed soaking
Soaking the seeds treated in the step in diethyl aminoethyl hexanoate solution with the concentration of 20-160mg/L, washing for many times, and airing;
3) seeding
The seeds treated above are sown in soil.
Further, in the step 1), the sodium hypochlorite solution is 0.8-1.0% (wt%); the soaking and disinfecting temperature is 24-26 ℃, and the soaking and disinfecting time is 8-9 min; 100g of seeds were soaked in 500ml of sodium hypochlorite solution.
Still further, the sodium hypochlorite solution was 0.9% (wt%).
Further, in the step 2), the seed soaking time is 4-6h at the temperature of 22-28 ℃ of the diethyl aminoethyl hexanoate solution; 100g of seeds are soaked in every 500ml of diethyl aminoethyl hexanoate solution.
Still further, in the step 2), the concentration of the diethyl aminoethyl hexanoate solution is 40 mg/L.
The invention has the beneficial effects that:
according to the invention, through simple seed soaking treatment of diethyl aminoethyl hexanoate, the leaf area (18% at most) of cotton seedlings can be remarkably increased, the germination potential (24% at most) and the emergence rate (8% at most) are increased, the relative chlorophyll content (SPAD) is increased (21% at most), and photosynthesis is increased to promote cotton biomass accumulation (37% at most) and strong seedlings. The stem thickness (26 percent at the most) and the seedling height (13 percent at the most) are improved; the number of the air holes is increased, so that photosynthesis and transpiration are facilitated, and biomass accumulation is improved; the root development is promoted, so that the competition and growth capacity of the cotton in the seedling stage are enhanced, the increase of the leaf area is favorable for quickly occupying the space, the growth of weeds is prevented to a certain extent, the problem of difficulty in sowing the whole seedling at one time can be solved by improving the germination vigor and the germination rate, and the production of the cotton is facilitated.
Detailed Description
The present invention is described in further detail below with reference to specific examples so that those skilled in the art can understand the invention.
Example 1 Effect of different concentrations of diethyl aminoethyl hexanoate on Cotton leaf area, Biomass accumulation, Germination and morphology
1. Test materials and methods
1.1 test materials
Hua Cotton 3097, bred and supplied by Huazhong university of agriculture. The test site is the photosulturing room of the third comprehensive building of Huazhong university of agriculture. Preparation of diethyl aminoethyl hexanoate (DA-6) reagent:
accurately weighing 100mg of DA-6 (a source leaf organism, the purity is 98%) and placing the weighed solution into a beaker, adding 50ml of pure water, stirring and dissolving the solution by a glass rod, then pouring the solution into a 100ml volumetric flask, slowly adding 5ml of pure water along the wall of the beaker (slowly rotating the beaker while adding water in the process) to wash the beaker, pouring the washing solution into the volumetric flask (repeatedly washing for three times), adding pure water to dissolve and fix the volume to 100ml, namely 1mg/ml of DA-6 mother solution, and hermetically storing the glass flask at 4 ℃:
DA 2: taking 2ml of DA-6 mother liquor, diluting to 100ml, 20mg/L
DA 4: taking 4ml of DA-6 mother liquor, diluting to 100ml, 40 mg/L;
DA 8: taking 8ml of DA-6 mother liquor, diluting to 100ml, 80 mg/L;
DA 16: taking 16ml of DA-6 mother liquor, diluting to 100ml and 160 mg/L.
1.2 methods
(1) Pretreatment
The cotton seeds are continuously sunned for 4-5 days, when the weather is clear, the cotton seeds are evenly paved on a wood board and a mat for tedding from 9 am to 3 pm, the seeds are paved to be 2-3 cm thick, the seeds are turned more to keep the weight and evenly sunning until the seeds are grabbed and kneaded to be the sound of sand, the water content is reduced to the same level, and the uniformity is ensured. The seeds can not be sunned on the cement ground, and the seeds are easy to be sunburned. Soaking 100g seeds in 0.9% sodium hypochlorite solution (24-26 deg.C) for 8-9min for sterilization, washing with distilled water for three times, sucking off water, spreading on newspaper, placing in 24-27 deg.C room for 30min, and volatilizing surface water (spreading 2-3 cm thick).
(2) Seed soaking
Soaking 100 seeds in 50ml of each treatment solution (24-26 ℃) at room temperature (24-26 ℃), soaking the seeds for 6 hours, covering the container opening with newspaper or A4 paper, slightly stirring to ensure that all cotton seeds can be soaked in the solution, taking out the seeds after soaking, washing the seeds for three times with clean water, and drying the seeds in the air.
(3) Seeding
Planting with vermiculite, pouring vermiculite into nutrition bowls (8cm × 7cm), the depth of vermiculite is 5cm, each bowl is filled with 3-4 seeds, covering with vermiculite, adding 1.5L clear water, covering with film, and removing film to spray small amount of clear water on the surface on the third day after sowing. Transplanting after sowing for 4 days. Each pot has 16 nutrition pots, and 60 grains are planted. After 4 days, the seedling pot is filled with water, the nutrition pot floats slightly, at this time, the cotton is taken out lightly and does not hurt the cotton root system, and the cotton seedling with even growth is transplanted into half amount of Hoagland nutrient solution (the nutrient solution formula is slightly adjusted but basically the same: 472.5mg/L of calcium nitrate tetrahydrate, 303.5mg/L of potassium nitrate, 57.5mg/L of ammonium dihydrogen phosphate, 246.5mg/L of magnesium sulfate heptahydrate, 3.475ug/L of ferrous sulfate heptahydrate, 4.66ug/L of disodium ethylenediamine tetraacetic acid, 0.83mg/L of potassium iodide, 3.1mg/L of boric acid, 11.2mg/L of manganese sulfate, 4.3mg/L of zinc sulfate, 0.13mg/L of sodium molybdate (0.125 mg/L original), 0.013mg/L of copper sulfate (0.0125 mg/L original), 0.013mg/L of cobalt chloride (0.0125 mg/L original), and the like for cultivation. And (3) field planting cotton, wrapping the root-tuber junction with the field planting cotton, slightly plugging the field planting cotton into planting holes of cotton field planting plates, field planting 6 cotton seedlings on each field planting plate, pouring 3L of half amount of Hoagland nutrient solution into a planting pot, and then placing the cotton field planting plates on which the 6 cotton seedlings are field planted on the planting pot. The pot is 20cm long, 20cm wide and 8cm deep. 2 strains were treated as 1 replicate with 6 replicates per treatment. Culturing in light culture room at 25.5-27.9 deg.C and humidity of 59.4% for 12 hr per day. Samples were taken on day 25 after sowing.
2. Statistical calculation
20 seeds were considered as one repeat, for a total of three. Taking the seed unearthing as a standard of seedling emergence, counting the number of sprouts on the third day, and calculating the germination vigor:
germination potential (%) × (cumulative germination number/seed amount on day 3) × 100%.
The SPAD of the fourth leaf was measured by SPAD instrument 25 days after sowing, and the average value was taken 5 times per leaf. And measuring the length from the top growing point to the position where rooting starts on the 25 th day after sowing, wherein the length is the height of the seedling. The thickness of the cotyledon in the flattened direction was measured as the thickness of the stem. All leaves of cotton seedlings were scanned using color flatbed scanner MRA-3200A3L and leaf area was calculated by FIJI software. Dividing cotton seedlings into roots, stems and leaves, putting the cotton seedlings into an oven for deactivation of enzymes at 105 ℃ for 30min, drying the cotton seedlings at 75 ℃, weighing dry weight and calculating specific leaf weight:
specific weight of leaf (mg/cm) 2 )＝Leaf Dry weight (mg/plant)/leaf area (cm) 2 Strain/strain).
3. Results and analysis
TABLE 1 Effect of seed soaking on various indexes of Cotton seedlings
Note: (the small letter a, b, c, d and the like have obvious difference, P is less than or equal to 0.05)
Results and analysis: compared with the control, the leaf area of the fresh amine ester seed soaking with different concentrations is improved by 10-17%, the SPAD value of the relative chlorophyll content is improved by 2-21%, and the total biomass accumulation is improved by 11-37%; the germination potential is improved by 0.5 to 20 percent, the stem thickness and the seedling height are respectively improved by 9 to 26 percent and 4 to 13 percent; the results show that: the diethyl aminoethyl hexanoate seed soaking has the effects of increasing the leaf area, cultivating strong seedlings and increasing the germination speed; wherein, the treatment effect of 40mg/L diethyl aminoethyl hexanoate (DA4) is the best, the leaf area is improved by 17 percent, the SPAD value of the relative chlorophyll content is improved by 21 percent, and the total biomass accumulation is improved by 37 percent; the germination potential is improved by 20 percent, and the stem thickness and the seedling height are respectively improved by 26 percent and 9 percent.
Example 2 Effect of the fresh Aminophenate solution seed soaking on leaf area, germination vigor, biomass accumulation, morphology, leaf stomatal number, leaf epidermal cell and relative chlorophyll content (photosynthesis)
1. Test materials and methods
1.1 test materials
And the Chinese cotton 3097, bred and supplied by the university of agriculture in Huazhong. The test site is the photosulturing room of the third comprehensive building of Huazhong university of agriculture.
DA4 Process for the preparation of reference example 1
1.2 methods (further tests on DA4 and Control (CK))
1) Pretreatment
The cotton seeds are continuously sunned for 4-5 days, when the weather is clear, the cotton seeds are evenly paved on a wood board and a mat for tedding from 9 am to 3 pm, the seeds are paved to be 2-3 cm thick, the seeds are turned more to keep the weight and evenly sunning until the seeds are grabbed and kneaded to be the sound of sand, the water content is reduced to the same level, and the uniformity is ensured. The seeds can not be sunned on the cement ground, and the seeds are easy to be sunburned. Soaking 100g of sun-planted seeds in 500ml of 0.9% sodium hypochlorite solution (24-28 deg.C) for 8-9min for sterilization, washing with distilled water for three times, sucking off water, spreading on newspaper, placing in 24-28 deg.C room for 30min, and volatilizing surface water (spreading 2-3 cm thick).
2) Seed soaking
Soaking 100 seeds in 50ml of each treatment solution, soaking seeds for 6h at 24-28 ℃, taking out the seeds after soaking, washing the seeds with clean water for three times, slightly stirring to ensure that all cotton seeds can be soaked in the solution, covering the container opening with newspaper or A4 paper, placing the container in a room at 24-28 ℃ for 30min, and airing the excessive moisture on the surface.
3) Seeding
Then planting the seeds with vermiculite, pouring the vermiculite into nutrition bowls (8cm x 7cm), wherein the depth of the vermiculite is 5cm, 3-4 seeds in each bowl are covered with the vermiculite, adding 1.5L of clear water, covering with a film, and uncovering the film to spray a small amount of clear water on the surface on the third day after sowing. Transplanting after sowing for 4 days. Each pot has 16 nutrition pots, and 60 grains are planted. After 4 days, the seedling pot is filled with water, the nutrition pot floats slightly, at this time, the cotton is taken out lightly and does not hurt the cotton root system, and the cotton seedling with even growth is transplanted into half amount of Hoagland nutrient solution (the nutrient solution formula is slightly adjusted but basically the same: 472.5mg/L of calcium nitrate tetrahydrate, 303.5mg/L of potassium nitrate, 57.5mg/L of ammonium dihydrogen phosphate, 246.5mg/L of magnesium sulfate heptahydrate, 3.475ug/L of ferrous sulfate heptahydrate, 4.66ug/L of disodium ethylenediamine tetraacetic acid, 0.83mg/L of potassium iodide, 3.1mg/L of boric acid, 11.2mg/L of manganese sulfate, 4.3mg/L of zinc sulfate, 0.13mg/L of sodium molybdate (0.125 mg/L original), 0.013mg/L of copper sulfate (0.0125 mg/L original), 0.013mg/L of cobalt chloride (0.0125 mg/L original), and the like for cultivation. And (3) field planting cotton, wrapping the root-tuber junction with the field planting cotton, slightly plugging the field planting cotton into planting holes of cotton field planting plates, field planting 6 cotton seedlings on each field planting plate, pouring 3L of half amount of Hoagland nutrient solution into a planting pot, and then placing the cotton field planting plates on which the 6 cotton seedlings are field planted on the planting pot. The pot is 20cm long, 20cm wide and 8cm deep. 2 strains were treated as 1 replicate with 6 replicates per treatment. Culturing in light culture room at 25.5-27.8 deg.C and humidity of 60.1% for 12 hr per day. Samples were taken on day 25 after sowing. For each treatment, 12 plants were planted, and 9 plants with uniform growth vigor were selected, and 3 plants were treated, and the three plants were regarded as three replicates. 6 strains were used for determining leaf area and dry weight, and 3 strains were used for determining microstructure.
2. Statistical calculation
20 seeds were considered as one repeat, for a total of three. Taking the seed unearthing as a standard of seedling emergence, counting the number of sprouts on the third day, and calculating the germination vigor:
germination potential (%) × (cumulative germination number/seed amount on day 3) × 100%. The SPAD of the fourth leaf was measured by SPAD instrument 25 days after sowing, and the average value was taken 5 times per leaf. And measuring the length from the top growing point to the position where rooting starts on the 25 th day after sowing, wherein the length is the height of the seedling. The thickness of the cotyledon in the flattened direction was measured as the thickness of the stem. All leaves of cotton seedlings were scanned using color flatbed scanner MRA-3200A3L and leaf area was calculated by FIJI software. Dividing cotton seedlings into roots, stems and leaves, putting the cotton seedlings into an oven for deactivation of enzymes for 30min at 105 ℃, drying the cotton seedlings at 75 ℃, weighing dry weight and calculating specific leaf weight:
specific weight of leaf (mg/cm) 2 ) Dry weight of leaf (mg/plant)/area of leaf (cm) 2 Strain/strain).
Preparing an upper epidermal slide and a lower epidermal slide of a fourth leaf by adopting a blotting method, taking three leaves every time, preparing three slides, taking three fields of view for each slide, observing and taking a picture by using an inverted fluorescence microscope, analyzing the number of upper epidermal cells and lower epidermal cells and the number of pores by using ImageJ software, and calculating the relative cell density, the relative pore density and the epidermal cell area of the upper epidermal cells and the lower epidermal cells:
relative cell density (pieces/mm) 2 ) Number of cells in field/area of field (mm) 2 )
Relative pore Density (pieces/mm) 2 ) Number of stomata in field of view/area of field of view (mm) 2 )
Epidermal cell area (um) 2 ) 1000000/relative cell density (pieces/mm) 2 )。
3. Results and analysis
TABLE 2 Effect of seed soaking on various indexes of Cotton seedlings
TABLE 3 Effect of seed soaking on Cotton leaf epidermal indicators
Results and analysis: compared with the control, the area of the leaf treated by soaking the diethyl aminoethyl hexanoate is improved by 13 percent, the content of the relative chlorophyll is improved by 13 percent, the total biomass accumulation is improved by 14 percent, the germination potential is improved by 5 percent, the stem thickness is improved by 12 percent, and the seedling height is improved by 7 percent. The relative density of the upper epidermis and the lower epidermis is increased, the relative pore density is unchanged, but the leaf area is increased, which shows that the total number of pores on one leaf is increased, thus being beneficial to promoting photosynthesis and transpiration and improving biomass accumulation.
The results show that: the 40mg/L diethyl aminoethyl hexanoate seed soaking treatment can achieve the effects of increasing the leaf area, improving the germination speed, increasing the biomass accumulation, cultivating strong seedlings and improving the photosynthesis.
Example 3 Effect of diethyl aminoethyl hexanoate solution seed soaking on leaf area, germination vigour, emergence rate, morphology, root system, biomass accumulation, leaf stomatal number, leaf epidermal cell and chlorophyll relative content (photosynthesis)
1. Test materials and methods
1.1 test materials
Hua Cotton 3097, bred and supplied by Huazhong university of agriculture. Is a common late-sowing variety in Hubei, and the test site is the photosulturing room of the third comprehensive building of the university of agriculture in Huazhong.
DA4 preparation the procedure of example 1 was followed
1.2 methods (further analytical screening of DA4 and Control (CK) on the basis of example 1)
(1) Pretreatment
The cotton seeds are continuously sunned for 4-5 days, when the weather is clear, the cotton seeds are evenly paved on a wood board and a mat for tedding from 9 am to 3 pm, the seeds are paved to be 2-3 cm thick, the seeds are turned more to keep the weight and evenly sunning until the seeds are grabbed and kneaded to be the sound of sand, the water content is reduced to the same level, and the uniformity is ensured. The seeds can not be sunned on the cement ground, and the seeds are easy to be sunburned. Soaking 100g seeds in 0.9% sodium hypochlorite solution (22-24 deg.C) for 8-10min for sterilization, washing with distilled water for three times, sucking off water, spreading on newspaper, placing in room at 22-25 deg.C for 30min, and volatilizing surface water (spreading 2-3 cm thick).
(2) Seed soaking
Soaking 100 seeds in 50ml of each treatment solution (22-24 deg.C), soaking for 6h, taking out the seeds after soaking, washing with clear water for three times, slightly stirring to immerse all cotton seeds in the solution, covering the container opening with newspaper or A4 paper, placing in a room at 22-25 deg.C for 30min, and air drying the excess water on the surface.
(3) Seeding
Then planting the seeds with vermiculite, pouring the vermiculite into nutrition bowls (8cm x 7cm), wherein the depth of the vermiculite is 5cm, 3-4 seeds in each bowl are covered with the vermiculite, adding 1.5L of clear water, covering with a film, and uncovering the film to spray a small amount of clear water on the surface on the third day after sowing. Transplanting after sowing for 4 days. Each pot has 16 nutrition pots, and 60 grains are planted. After 4 days, the seedling pot is filled with water, the nutrition pot floats slightly, the cotton is taken out gently and does not hurt the root system of the cotton, and the cotton seedling with even growth is transplanted into half amount of Hoagland nutrient solution (the nutrient solution formula is slightly adjusted but basically the same: 472.5mg/L of calcium nitrate tetrahydrate, 303.5mg/L of potassium nitrate, 57.5mg/L of ammonium dihydrogen phosphate, 246.5mg/L of magnesium sulfate heptahydrate, 3.475ug/L of ferrous sulfate heptahydrate, 4.66ug/L of disodium ethylenediamine tetraacetic acid, 0.83mg/L of potassium iodide, 3.1mg/L of boric acid, 11.2mg/L of manganese sulfate, 4.3mg/L of zinc sulfate, 0.13mg/L of sodium molybdate (0.125 mg/L originally), 0.013mg/L of copper sulfate (0.0125 mg/L originally), and 0.013mg/L of cobalt chloride (0.0125 mg/L originally)) for cultivation. And (3) field planting cotton, wrapping the root-tuber junction with the field planting cotton, slightly plugging the field planting cotton into planting holes of cotton field planting plates, field planting 6 cotton seedlings on each field planting plate, pouring 3L of half amount of Hoagland nutrient solution into a planting pot, and then placing the cotton field planting plates on which the 6 cotton seedlings are field planted on the planting pot. The pot is 20cm long, 20cm wide and 8cm deep. 2 strains were treated as 1 replicate with 6 replicates per treatment. Culturing in light culture room at 24.2-26.2 deg.C and humidity of 55.5% for 12 hr per day. Samples were taken on day 25 after sowing. For each treatment, 12 plants were planted, and 9 plants with uniform growth vigor were selected, and 3 plants were treated, and the three plants were regarded as three replicates. 6 strains were used for determining leaf area and dry weight, and 3 strains were used for determining microstructure.
2. And (3) statistical calculation:
20 seeds were considered as one repeat, for a total of three. Taking the seed unearthed as a standard of seedling emergence, counting the number of the seedlings on the third day, and calculating the germination vigor and the seedling emergence rate:
germination rate (%) is (cumulative number of sprouts/seeding amount on day 3) × 100%, and emergence rate (%) (cumulative number of seedlings/seeding amount on day 7) × 100%.
The SPAD of the fourth leaf was measured by SPAD instrument 25 days after sowing, and the average value was taken 5 times per leaf. And measuring the length from the top growing point to the position where rooting starts on the 25 th day after sowing, wherein the length is the height of the seedling. The thickness of the cotyledon in the flattened direction was measured as the thickness of the stem. All leaves of cotton seedlings were scanned using color flatbed scanner MRA-3200A3L and leaf area was calculated by FIJI software. Cotton root lines were individually scanned using a color flatbed scanner MRA-3200A3L and analyzed for root length, root surface area, root volume, mean diameter and root tip number by LA-S software. Dividing cotton seedlings into roots, stems and leaves, putting the cotton seedlings into an oven for deactivation of enzymes at 105 ℃ for 30min, drying the cotton seedlings at 75 ℃, weighing dry weight and calculating specific leaf weight:
specific weight of leaf (mg/cm) 2 ) Dry weight of leaf (mg/plant)/area of leaf (cm) 2 Strain/strain).
Preparing an upper epidermal slide and a lower epidermal slide of a fourth leaf by adopting a blotting method, taking three leaves every time, preparing three slides, taking three fields of view for each slide, observing and taking a picture by using an inverted fluorescence microscope, analyzing the number of upper epidermal cells and lower epidermal cells and the number of pores by using ImageJ software, and calculating the relative cell density, the relative pore density and the epidermal cell area of the upper epidermal cells and the lower epidermal cells:
relative cell density (pieces/mm) 2 ) Number of cells in field/area of field (mm) 2 )
Relative pore Density (pieces/mm) 2 ) Number of stomata in field of view/area of field of view (mm) 2 )
Epidermal cell area (um) 2 ) 1000000/relative cell density (pieces/mm) 2 )
3. Results and analysis
TABLE 4 Effect of seed soaking on various indexes of Cotton seedlings
TABLE 5 Effect of seed soaking on various indexes of Cotton seedlings
Results and analysis: compared with the control, the leaf area of the 40mg/L diethyl aminoethyl hexanoate seed soaking is improved by 18 percent, the SPAD is improved by 12 percent, the total biomass accumulation is improved by 22 percent, the germination potential is improved by 24 percent, the emergence rate is improved by 8 percent, the stem thickness and the seedling height are respectively improved by 2 to 3 percent, the development of root system morphology is promoted, and the cell density and the pore density of the upper epidermis and the lower epidermis are improved; the improvement of the pore density is beneficial to promoting photosynthesis and transpiration, is beneficial to substance accumulation, promotes the improvement of leaf area and morphological development, and cultivates strong seedlings.
Other parts not described in detail are prior art. Although the present invention has been described in detail with reference to the above embodiments, it is only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments, and the embodiments are within the scope of the present invention.
Claims (10)
1. An application of diethyl aminoethyl hexanoate in cotton planting is disclosed.
2. An application of diethyl aminoethyl hexanoate in increasing leaf area, increasing germination speed and rate and increasing photosynthesis to promote biomass accumulation in the course of cotton planting.
3. The seed initiator for increasing the leaf area of cotton in seedling stage, raising germination rate and raising photosynthesis to promote biomass accumulation is characterized by comprising the following components in parts by weight: the seed initiator comprises diethyl aminoethyl hexanoate.
4. A seed initiator according to claim 3, characterized in that: in the seed initiator, the content of diethyl aminoethyl hexanoate is 20-160 mg/L.
5. A seed initiator according to claim 3, characterized in that: the content of diethyl aminoethyl hexanoate is 40 mg/L.
6. A method for increasing the leaf area of cotton in seedling stage, increasing the germination speed and rate, and improving photosynthesis to promote biomass accumulation is characterized in that: the method comprises the following steps:
1) pretreatment
Uniformly tedding cotton seeds, then soaking the cotton seeds in a sodium hypochlorite solution, washing and airing;
2) seed soaking
Soaking the seeds treated in the step in diethyl aminoethyl hexanoate solution with the concentration of 20-160mg/L, washing for many times, and airing;
3) seeding
The seeds treated above are sown in soil.
7. The method of claim 6, wherein: in the step 1), the sodium hypochlorite solution is 0.8-1.0%; the soaking and disinfecting temperature is 24-26 ℃, and the soaking and disinfecting time is 8-9 min; 100g of seeds were soaked in 500ml of sodium hypochlorite solution.
8. The method of claim 6, wherein: the sodium hypochlorite solution was 0.9%.
9. The method of claim 6, wherein: in the step 2), the seed soaking time is 4-6h at 22-28 ℃ of the diethyl aminoethyl hexanoate solution; 100g of seeds are soaked in every 500ml of diethyl aminoethyl hexanoate solution.
10. The method of claim 9, wherein: in the step 2), the concentration of the diethyl aminoethyl hexanoate solution is 40 mg/L.
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