CN115054665A

CN115054665A - Traditional Chinese medicine composition for treating eczema scroti

Info

Publication number: CN115054665A
Application number: CN202210953718.7A
Authority: CN
Inventors: 叶静静
Original assignee: Ningbo Traditional Chinese Medicine Hospital
Current assignee: Ningbo Traditional Chinese Medicine Hospital
Priority date: 2022-08-10
Filing date: 2022-08-10
Publication date: 2022-09-16
Anticipated expiration: 2042-08-10
Also published as: CN115054665B

Abstract

The invention relates to a traditional Chinese medicine composition for treating scrotal eczema, which is prepared from the following traditional Chinese medicines: sweet wormwood, scutellaria baicalensis, lophatherum gracile, ricepaperplant pith, radix rehmanniae, poria cocos, indigo naturalis, talc and liquorice. The traditional Chinese medicine composition disclosed by the invention has the advantages that the itching relieving rate of scrotal eczema is high, the recurrence rate is low, the adverse reaction is rare, and the external treatment is carried out by matching with the scrotal wet dressing bag and the LED green light irradiation, so that the traditional Chinese medicine composition can dredge qi of the liver and gallbladder damp-heat channels, astringe and excrete dampness, lead pathogenic factors out, and promote the healing of local skin damage.

Description

Traditional Chinese medicine composition for treating eczema scroti

Technical Field

The invention relates to the field of traditional Chinese medicines, and in particular relates to a traditional Chinese medicine composition for treating eczema scroti.

Background

Eczema is an inflammation of the epidermis and the superficial dermis caused by multiple internal and external factors. Clinically, acute eczema and chronic eczema are divided according to the onset time, and generalized eczema and limited eczema are divided according to the onset position. The ancient books of traditional Chinese medicine have been known for thousands of years, the records of the ancient books of traditional Chinese medicine are mainly classified into 'acute eczema', 'damp toxin', 'damp sore' and the like, and the damp toxin sore is formed by that the muscles are not full due to the deficiency of spleen and stomach and the downward flow of damp heat; or due to storm, rain, cold, dampness and summer heat invading the skin, the related Chinese medicinal preparation can obviously improve the clinical symptoms of eczema patients, and the obvious clinical curative effect and safety are valued by the medical field.

Eczema of scrotum is a common skin disease, clinically, the skin disease mainly comprises local dark erythema, pachynsis and surface roughness, and is accompanied by pruritus of different degrees and recurrent attacks. The disease can occur in all seasons and at any age stage. Due to changes of climate environment and dietary structure, acceleration of life rhythm, increase of mental stress, application of a large amount of chemicals and the like, in recent years, the incidence rate of eczema scroti is on the rise, and patients with eczema scroti are special in position, often accompanied with severe pruritus and easy to repeat the course of disease, so that great trouble is brought to the life and work of the patients.

The clinical treatment method for eczema scroti is various, at present, antihistamine, vitamins and sedative hypnotic drugs are commonly used in western medicine and are matched with external drugs such as corticosteroids, immunosuppressant and the like for treatment, but related side effects or potential risks exist.

The existing traditional Chinese medicine formula for treating scrotal eczema has limited treatment effect, so that medicines applied to treating scrotal eczema are further developed from the field of traditional Chinese medicines, richer choices are provided for formulating clinical medication schemes, and the technical problem to be solved is urgently needed.

Disclosure of Invention

The invention aims to provide a traditional Chinese medicine composition capable of effectively treating eczema scroti.

In order to achieve the purpose, the invention adopts the following technical scheme:

the invention provides a traditional Chinese medicine composition for treating eczema scroti, which is prepared from the following traditional Chinese medicines: sweet wormwood, scutellaria baicalensis, lophatherum gracile, ricepaperplant pith, radix rehmanniae, poria cocos, indigo naturalis, talc and liquorice.

Preferably, the traditional Chinese medicine composition is prepared from the following traditional Chinese medicines in parts by weight: 20-40 parts of sweet wormwood, 5-25 parts of scutellaria baicalensis, 20-40 parts of lophatherum gracile, 1-10 parts of ricepaper pith, 20-40 parts of radix rehmanniae, 5-25 parts of poria cocos, 1-10 parts of indigo naturalis, 20-40 parts of talc and 1-10 parts of liquorice.

More preferably, the traditional Chinese medicine composition is prepared from the following traditional Chinese medicines in parts by weight: 25-35 parts of sweet wormwood, 10-20 parts of scutellaria baicalensis, 25-35 parts of lophatherum gracile, 3-8 parts of ricepaper pith, 25-35 parts of radix rehmanniae, 10-20 parts of poria cocos, 3-8 parts of indigo naturalis, 25-35 parts of talc and 3-8 parts of liquorice.

Most preferably, the traditional Chinese medicine composition is prepared from the following traditional Chinese medicines in parts by weight: 30 parts of sweet wormwood, 15 parts of baical skullcap root, 30 parts of lophatherum gracile, 5 parts of ricepaperplant pith, 30 parts of radix rehmanniae, 15 parts of poria cocos, 6 parts of indigo naturalis, 30 parts of talcum and 6 parts of liquorice.

Preferably, the traditional Chinese medicine composition is an external preparation or an oral preparation.

More preferably, the external preparation is an ointment, a gel, a liniment, a lotion or a patch, and the oral preparation is a granule, a tablet, a pill or a decoction.

The second aspect of the present invention provides a preparation method of the above traditional Chinese medicine composition, comprising the following steps:

placing the sweet wormwood, the baical skullcap root, the lophatherum gracile, the ricepaperplant pith, the rehmannia root, the poria cocos, the natural indigo, the talc and the liquorice in the prescription amount into a decoction container, adding 2-4 times of water by weight, soaking for 30 minutes, decocting with strong fire until the materials are boiled, decocting with slow fire for 30-60 minutes, filtering, and concentrating the filtrate to 1/3 of the original volume of the added distilled water.

Preferably, 3 times the weight of water is added in the preparation method.

Preferably, the decoction time with slow fire in the preparation method is 45 minutes.

The third aspect of the invention is to provide the application of the traditional Chinese medicine composition in preparing a medicine for treating eczema.

Preferably, the eczema is eczema of scrotum.

The fourth aspect of the invention provides application of the traditional Chinese medicine composition in preparing a medicine for treating pruritus.

Preferably, the pruritus is pruritus associated with eczema.

The fifth aspect of the invention provides the application of the traditional Chinese medicine composition in preparing medicines for reducing the level of IL-31 in serum and/or increasing the level of IFN-gamma in serum.

Preferably, the invention provides application of the traditional Chinese medicine composition in preparing a medicine for simultaneously reducing the level of IL-31 in serum and increasing the level of IFN-gamma in serum.

The common pharmacological effects of the traditional Chinese medicine raw materials selected by the invention in the field are as follows:

sweet wormwood herb: it is cold in nature, bitter and pungent in flavor, and enters liver and gallbladder meridians. The sweet wormwood herb has the effects of clearing away summer heat, removing heat, and preventing malaria, and is clinically used for summer-heat pathogen fever, yin deficiency fever, night fever and early coolness, bone steaming and fatigue fever, malaria chills and fever, and damp-heat jaundice.

Scutellaria baicalensis: it is cold in nature and bitter in taste, and enters lung, gallbladder, spleen, large intestine and small intestine meridians. The scutellaria baicalensis clears heat and dries dampness, purges fire and detoxifies, stops bleeding and prevents abortion, and is clinically used for chest distress and nausea, damp-heat fullness, dysentery, jaundice, cough with lung heat, high fever and polydipsia, blood heat and epistaxis, carbuncle, swelling and sore toxicity and threatened abortion due to damp-warm and summer-warm diseases.

Herba lophatheri: cold in nature, sweet and bland in flavor, they enter heart, stomach and small intestine meridians. Lophatherum gracile has the effects of clearing heat, relieving restlessness and promoting urination, and is clinically used for treating fever, polydipsia, dark urine, stranguria pain and aphtha of the mouth and tongue.

Ricepaperplant pith: slightly cold in nature, sweet and bland in flavor, entering lung and stomach meridians. Medulla Tetrapanacis has effects of clearing heat, promoting diuresis, and promoting qi and lactation, and can be used for treating damp-heat with dark urine, gonorrhea, pain, edema, oliguria, and galactostasis.

The root of rehmannia: cold in nature and sweet in flavor, it enters heart, liver and kidney meridians. The dried rehmannia root has the effects of clearing heat and cooling blood, nourishing yin and promoting the production of body fluid, and is clinically used for treating fever with deep-red tongue and polydipsia, yin deficiency and internal heat, bone steaming and fatigue heat, internal heat diabetes, hematemesis and epistaxis, and macula and eruption.

Tuckahoe, poria cocos: neutral in nature, sweet and bland in flavor, and entering heart, lung, spleen and kidney meridians. Poria has effects in promoting diuresis, eliminating dampness, invigorating spleen, and calming heart, and can be used for treating edema, oliguria, dizziness, palpitation, spleen deficiency, anorexia, loose stool, diarrhea, uneasiness, palpitation, and insomnia.

Indigo naturalis: cold in nature, salty in taste, entering liver meridian. Indigo naturalis has effects of clearing heat and cooling blood, and arresting convulsion, and can be used for treating warm toxin, macula, blood heat hematemesis, chest pain hemoptysis, aphtha, mumps, pharyngitis, infantile convulsion, etc.

Talc: cold in nature, sweet and bland in flavor, it enters bladder, lung and stomach meridians. Talcum has effects of promoting diuresis for treating stranguria, clearing summerheat, eliminating dampness and healing sore for external use, and can be used for treating stranguria due to heat, stranguria caused by stone, urine heat pain, summer-heat dampness polydipsia, damp-heat watery diarrhea, eczema, and miliaria.

Licorice root: mild in nature and sweet in taste. The licorice has the effects of tonifying qi and strengthening middle warmer, relieving spasm and pain, moistening lung and relieving cough, purging pathogenic fire and removing toxicity, and harmonizing the drugs, and is clinically used for treating listlessness and poor appetite, emaciation and sallow complexion, palpitation and shortness of breath, abdominal pain and loose stool, limb spasm and acute pain, hysteria, cough and asthma, sore throat, carbuncle and sore, infantile fetal toxicity, and drug and food poisoning.

The invention has the advantages that:

1. the compatibility of the traditional Chinese medicine composition conforms to the principle of monarch, minister, assistant and guide of the traditional Chinese medicine, sweet wormwood herb is used for clearing shaoyang pathogenic heat, scutellaria baicalensis is used for clearing gallbladder heat and drying dampness, and the combination of the two medicines is used as a monarch medicine which can clear shaoyang damp-heat and eliminate pathogenic factors. Lophatherum gracile is sweet and bland, clears heart and eliminates restlessness, is bland in nature and facilitates defecation and urination, guides heart fire to descend, is bitter and cold in ricepaper pith, enters heart channels and small intestine channels, clears heart fire in the upper part, guides heat of small intestine in the lower part, is sweet and cold in the radix rehmanniae, cools blood, nourishes yin and reduces fire, is combined as a ministerial drug, guides fire to descend without damaging kidney yin.

2. The traditional Chinese medicine composition provided by the invention has the advantages of reasonable medicine design, mutual compatibility, high pruritus relieving rate on eczema scroti, low recurrence rate and fresh adverse reaction.

3. The traditional Chinese medicine composition is matched with a scrotum wet compress bag and LED green light irradiation for external treatment, and can dredge qi of the liver and gallbladder damp-heat channels, astringe and excrete dampness, lead evil out and promote local skin damage healing.

Detailed Description

The present invention will be further described with reference to the following examples, but the embodiments of the present invention are not limited thereto. The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.

Example 1

30g of sweet wormwood herb, 15g of baical skullcap root, 30g of common lophatherum herb, 5g of ricepaper pith, 30g of rehmannia root, 15g of poria cocos, 6g of natural indigo, 30g of talc and 6g of raw licorice are respectively weighed, water with the weight of 3 times is added, after the mixture is soaked for 30 minutes, the mixture is decocted by strong fire to boil, and then decocted by slow fire for 45 minutes, the mixture is filtered, and the filtrate is concentrated to 1/3 of the volume of the original added distilled water, so that the traditional Chinese medicine composition 1 is obtained.

Example 2

Weighing 15g of sweet wormwood, 30g of baical skullcap root, 30g of common lophatherum herb, 5g of ricepaper pith, 30g of rehmannia root, 15g of tuckahoe, 6g of natural indigo, 30g of talcum and 6g of raw licorice, and obtaining the traditional Chinese medicine composition 2 by the same preparation method and steps as the example 1.

Example 3

30g of sweet wormwood, 15g of baical skullcap root, 15g of common lophatherum herb, 5g of ricepaper pith, 30g of rehmannia root, 30g of tuckahoe, 6g of natural indigo, 30g of talcum and 6g of raw liquorice are respectively weighed, and the rest preparation method and steps are the same as those in example 1, so that the traditional Chinese medicine composition 3 is obtained.

Example 4

30g of sweet wormwood, 15g of baical skullcap root, 30g of common lophatherum herb, 5g of ricepaper pith, 15g of rehmannia root, 30g of tuckahoe, 6g of natural indigo, 30g of talcum and 6g of raw liquorice are respectively weighed, and the rest preparation method and steps are the same as those in example 1, so that the traditional Chinese medicine composition 4 is obtained.

Example 5

30g of sweet wormwood, 15g of baical skullcap root, 30g of common lophatherum herb, 5g of ricepaper pith, 30g of rehmannia root, 30g of Indian buead, 6g of natural indigo, 15g of talcum and 6g of raw liquoric root are respectively weighed, and the rest preparation method and steps are the same as those in example 1, so that the traditional Chinese medicine composition 5 is obtained.

Comparative example 1 (Replacing sweet wormwood into rhubarb)

Respectively weighing 30g of rheum officinale, 15g of scutellaria baicalensis, 30g of lophatherum gracile, 5g of ricepaperplant pith, 30g of radix rehmanniae, 15g of poria cocos, 6g of indigo naturalis, 30g of talc and 6g of raw liquorice, and obtaining the comparative example traditional Chinese medicine composition 1 by the same preparation method and steps as in example 1.

COMPARATIVE EXAMPLE 2 (Scutellaria baicalensis is replaced by phellodendron amurense)

30g of sweet wormwood herb, 15g of golden cypress, 30g of lophatherum gracile, 5g of ricepaper pith, 30g of rehmannia root, 15g of poria cocos, 6g of indigo naturalis, 30g of talc and 6g of raw liquorice are weighed respectively, and the rest preparation methods and steps are the same as those in example 1, so that the traditional Chinese medicine composition 2 in the comparative example is obtained.

Comparative example 3 (bamboo leaves replaced by fragrant solomonseal rhizome)

30g of sweet wormwood, 15g of baical skullcap root, 30g of fragrant solomonseal rhizome, 5g of ricepaper pith, 30g of rehmannia root, 15g of Indian buead, 6g of natural indigo, 30g of talcum and 6g of raw liquoric root are respectively weighed, and the rest preparation method and steps are the same as those in example 1, so that the traditional Chinese medicine composition 3 in the comparative example is obtained.

Test example 1 therapeutic effect of the Chinese medicinal composition of the present invention on rat eczema model

1. Test method

1.1 animal model preparation and dosing regimens

100 rats are taken and bred for one week in a conventional way, and then are randomly divided into 10 groups, namely a blank control group, a model group, 1-5 groups of the traditional Chinese medicine composition and 1-3 groups of the comparative example, wherein each group comprises 10 rats. After 2% sodium pentobarbital is administered to rats in each test group for intraperitoneal injection anesthesia, the back of each rat is marked with A, B places, the area of the place A is about 0.5cm multiplied by 0.5cm, the area of the place B is about 1cm multiplied by 1cm, the two places are separated by 2cm, the rat hair is scraped by a skin preparation knife, 6% sodium sulfide is smeared on the hair shaving area for 5 minutes, and then the sodium sulfide solution is wiped off by gauze dipping water. Except for the blank control group, the remaining 9 groups of dehaired rats were sensitized with a 7% solution of 2, 4-dinitro-chlorobenzophenone in the shaved area a. After 2 weeks, the site B was coated with a 0.1% solution of 2, 4-dinitrochloropropiophenone and challenged 1 time per week for a total of 4 weeks. On the next day after the successful model building, the rats in the groups 1 to 5 of the invention and the groups 1 to 3 of the comparative examples are respectively administered with the traditional Chinese medicine decoction prepared in the groups 1 to 5 of the invention and the groups 1 to 3 of the comparative examples by intragastric administration, the intragastric administration dose is 5ml/kg each time, the model group is administered with the normal saline with the same dose by intragastric administration for 2 times a day, and the treatment is continuously carried out for 2 weeks.

1.2 Observation index and evaluation method

The skin damage curative effect evaluation method comprises the following steps: scoring of skin lesions was performed by EASI scoring: the clinical manifestations are divided into four terms, namely: erythema (E), sclerema (edema)/papule (I), Exfoliation (EX), and lichenification (L). The severity of each clinical presentation is scored on a scale of 0-3, 0 ═ none, 1 ═ light, 2 ═ medium, 3 ═ heavy, and half-cuts between the various symptom scores, i.e., 0.5. Severity the following definition was used in Berth-Jones in the SASSAD (six area six sign atopic demattitis) severity scoring method: the score is not 0, and the physical sign cannot be determined after careful observation; the mild is 1 point, and the physical signs exist certainly but can be seen only by careful observation; 2 points in the middle, this sign can be seen immediately; with a weight of 3 points, this sign is very obvious. The judgment standard of the skin lesion curative effect is executed according to the guiding principle of clinical research of new traditional Chinese medicine, and the curative effect is judged according to the change of the comprehensive scores (namely the average value of four index scores of erythema, edema, epidermal exfoliation and bryozoasis) after treatment.

Detecting the content of IL-31 and IFN-gamma in serum: after the rats of each test group are fed with the drug last time, the rats are fasted without water prohibition, the rats are anesthetized with ether after 12 hours, the abdominal cavities of the rats are opened after the anesthesia is completed, tissues around the abdominal aorta are carefully stripped, the abdominal aorta is fully exposed, the abdominal aorta is subjected to abdominal aorta blood sampling by using a disposable venous blood taking needle and a vacuum blood storage vessel, the abdominal aorta is centrifuged at 2500r/min for 20min, serum is separated, the IL-31 and IFN-gamma content in the serum of the rats of each group is measured by adopting an ELISA method, and the specific operation method is operated according to the detailed description of the kit.

2. Test results

2.1 Effect on damaged skin

After 2 weeks of treatment of the eczema model rats with the Chinese medicinal composition of the invention, the damaged skin comprehensive scores of the rats in each test group are shown in table 1.

Table 1 comparison of the damaged skin composite scores of rats in each test group after treatment (n ═ 10)

As can be seen from the test data in the above table 1, the skin damage comprehensive score of the model group rats is obviously higher than that of the blank control group rats, and the chronic eczema-like skin damage changes appear on the backs of the model group rats, which accord with the clinical diagnosis standard of chronic eczema and indicate that the model building of the chronic eczema rats is successful. The comprehensive skin lesion scores in the examples 1 to 5 are obviously lower than those in the model group and the comparative examples 1 to 3, which shows that the traditional Chinese medicine composition can effectively relieve the skin lesion degree of the affected part of a chronic eczema model rat, relieve erythema, infiltration, scale and moss-like change, and has obvious curative effect on clinically treating scrotal eczema.

2.2 Effect on immunoinflammatory factors

After 2 weeks of treatment of the eczema model rats with the traditional Chinese medicine composition, the contents of IL-31 and IFN-gamma in the serum of the rats of each test group are shown in the table 2.

Table 2 comparison of IL-31 and IFN- γ concentration levels in rat serum of each test group (n ═ 10)

IL-31 is a member of the interleukin 6 family, and numerous studies have shown that IL-31, as a key link between Th2 cells and sensory nerves, is involved in the process of skin itch by activating IL-31RA functional receptors on sensory nerve cells and inducing LTB4 expression in keratinocytes, resulting in itch, and that after IL-31 signaling is inhibited, the patient's symptoms of itch are alleviated and well tolerated. Therefore, IL-31 and a signal path thereof play a crucial role in the mechanism of eczema pruritus and can be used as a potential target for treating skin diseases with pruritus as a main symptom.

As can be seen from the test results in Table 2 above, the IL-31 content in the serum of the rat in the model group is significantly increased compared with that in the blank control group (P < 0.01); IL-31 levels in the serum of rats in the groups of examples 1-5 were significantly reduced (P < 0.01) compared to the model group and the groups of comparative examples 1-3. The test results show that the traditional Chinese medicine composition provided by the embodiments 1-5 can effectively reduce the level of IL-31 in serum, and indicates that the traditional Chinese medicine composition has a remarkable curative effect in clinically improving pruritus symptoms caused by eczema scroti.

The cytokine IFN-gamma secreted by the Th1 cell not only can promote the maturation of the Th1 cell, but also can inhibit the production of IgE by the B lymphocyte and reduce the occurrence of allergic reaction. In the process of chronic eczema, Th1 is dominant and can inhibit the generation of allergy and the development of inflammation, and IFN-gamma is an important index reflecting the participation degree of Th1 cellular immune response and plays an important role in the generation and the development of allergic inflammatory diseases.

The test results in Table 2 show that the IFN-gamma content in the serum of the rat in the model group is obviously reduced compared with that in the blank control group (P is less than 0.01); the IFN-gamma content in the serum of rats in the groups of examples 1-5 is obviously increased (P < 0.01) compared with the model group and the groups of comparative examples 1-3. The test results show that the traditional Chinese medicine composition provided by the embodiments 1-5 can effectively improve the IFN-gamma level in serum, and indicates that the traditional Chinese medicine composition can relieve the anaphylactic reaction of chronic eczema by regulating Th1 type cellular immune expression, and remarkably relieves the clinically relevant inflammatory symptoms of eczema scroti.

Claims

1. A traditional Chinese medicine composition for treating eczema scroti is characterized by being prepared from the following traditional Chinese medicines: sweet wormwood, scutellaria baicalensis, lophatherum gracile, ricepaperplant pith, radix rehmanniae, poria cocos, indigo naturalis, talc and liquorice.

2. The traditional Chinese medicine composition according to claim 1, which is prepared from the following traditional Chinese medicines in parts by weight: 20-40 parts of sweet wormwood, 5-25 parts of scutellaria baicalensis, 20-40 parts of lophatherum gracile, 1-10 parts of ricepaper pith, 20-40 parts of radix rehmanniae, 5-25 parts of poria cocos, 1-10 parts of indigo naturalis, 20-40 parts of talc and 1-10 parts of liquorice.

3. The traditional Chinese medicine composition according to claim 2, which is prepared from the following traditional Chinese medicines in parts by weight: 25-35 parts of sweet wormwood, 10-20 parts of scutellaria baicalensis, 25-35 parts of lophatherum gracile, 3-8 parts of ricepaper pith, 25-35 parts of radix rehmanniae, 10-20 parts of poria cocos, 3-8 parts of indigo naturalis, 25-35 parts of talc and 3-8 parts of liquorice.

4. The traditional Chinese medicine composition according to claim 3, which is prepared from the following traditional Chinese medicines in parts by weight: 30 parts of sweet wormwood, 15 parts of baical skullcap root, 30 parts of lophatherum gracile, 5 parts of ricepaperplant pith, 30 parts of radix rehmanniae, 15 parts of poria cocos, 6 parts of indigo naturalis, 30 parts of talcum and 6 parts of liquorice.

5. The traditional Chinese medicine composition according to any one of claims 1 to 4, wherein the traditional Chinese medicine composition is an external preparation or an oral preparation.

6. The Chinese medicinal composition according to claim 5, wherein the external preparation is an ointment, a gel, a liniment, a lotion or a patch.

7. The traditional Chinese medicine composition of claim 5, wherein the oral preparation is a granule, a tablet, a pill or a decoction.

8. The method for preparing the traditional Chinese medicine composition of any one of claims 1 to 7, which is characterized by comprising the following steps: putting the sweet wormwood, the baical skullcap root, the lophatherum gracile, the ricepaperplant pith, the rehmannia root, the poria cocos, the natural indigo, the talc and the liquorice in the prescription amount into a decoction container, adding 2-4 times of water by weight, soaking for 30 minutes, decocting with strong fire until the mixture is boiled, decocting with slow fire for 30-60 minutes, filtering, and concentrating the filtrate to 1/3 of the original volume of the added distilled water.

9. Use of a Chinese medicinal composition according to any one of claims 1 to 7 in the manufacture of a medicament for the treatment of eczema.

10. The traditional Chinese medicine composition of claim 9, wherein the eczema is eczema of scrotum.