CN115054554B

CN115054554B - Five-finger wild peach no-wash hand cleanser and preparation method thereof

Info

Publication number: CN115054554B
Application number: CN202210850495.1A
Authority: CN
Inventors: 杨琳; 张君诚; 洪玉莹; 张杭颖; 章立; 吴进华
Original assignee: Sanming University
Current assignee: Sanming University
Priority date: 2022-07-20
Filing date: 2022-07-20
Publication date: 2024-03-22
Anticipated expiration: 2042-07-20
Also published as: CN115054554A

Abstract

The invention provides a five-finger wild peach hand-washing-free liquid soap and a preparation method thereof, wherein the method comprises the following steps: firstly, cleaning, drying and grinding roots of Ficus simplicissima lour to obtain Ficus simplicissima lour powder, then dissolving the Ficus simplicissima lour powder in ethanol, ultrasonically extracting, centrifuging and discarding precipitate to obtain Ficus simplicissima lour ethanol extract; mixing the ethanol extract of Ficus simplicissima lour with glycerol to obtain the washing-free hand-washing liquid. The invention adopts an 80% ethanol-ultrasonic method to extract the active ingredients of the root of the Ficus simplicissima lour, and the active ingredients are mixed with glycerol to prepare the Ficus simplicissima lour hand-washing liquid. Wherein, the five-finger wild peach is a weak acid natural Chinese herbal medicine substance, and the wild peach is added into the hand-washing-free liquid as an active ingredient, so that the concentration of ethanol in the hand-washing-free liquid can be reduced, the damage of the ethanol to the skin of the hand can be reduced, and the antibacterial effect can be increased. In addition, the five-finger nectarine can also endow the hand-washing-free liquid soap with a certain fragrance so as to improve the use feeling of a user.

Description

Five-finger wild peach no-wash hand cleanser and preparation method thereof

Technical Field

The invention relates to the technical field of hand-washing-free liquid soap, and in particular relates to a five-finger wild peach hand-washing-free liquid soap and a preparation method thereof.

Background

The no-clean hand sanitizer is known because water is not needed in the cleaning process, so that the steps of foaming and cleaning can be saved, and the no-clean hand sanitizer is convenient, practical and safe, and has the effect which is not inferior to that of the conventional hand sanitizer. For example, in general, the contact surface of the hand and the hand washing-free liquid is the pump head of the bottle, and the hand washing-free liquid is not easy to clean and avoid secondary infection because of being polluted and sterilized by solids such as soap, perfumed soap and the like. The hand-washing-free liquid soap has obvious sterilization effect, can achieve the sterilization rate of more than 90 percent on candida albicans, staphylococcus aureus, escherichia coli, hepatitis A and other diseases, has no irritation and anaphylactic reaction to human bodies, and can effectively protect families and themselves from bacteria and diseases. More importantly, the hand-washing-free liquid soap is used without water, and can be repeatedly used under the condition of spraying a few hands, so that the hand-washing-free liquid soap is quite convenient to use, and is often used in environments without water sources, such as hospitals, restaurants, families, institutions, train stations, travel, troops, entertainment venues, universities, elementary schools, kindergartens, supermarkets, hotels, banks, airports, wharfs, cinema and the like, for carrying out the water-free hand-washing-free disinfection.

At present, most of the hand-washing-free liquid soap mainly comprises alcohol. The basic formula of the alcohol-containing hand-washing-free liquid is generally alcohol plus glycerol, and a few essence for adjusting the fragrance is added. Wherein, alcohol is used as a disinfection component, acts on hands to perform disinfection, then volatilizes automatically, does not need to be washed by water, can meet the requirement of quick drying, and glycerol keeps certain moisture on skin to perform the effects of moisturizing skin. With the increasing importance of consumers on hand hygiene and skin protection, the market of the hand-washing-free hand-washing liquid is expanding, and the hand-washing-free hand-washing liquid has a certain application prospect. The common hand-washing-free liquid has the effects of sterilizing and inhibiting bacterial growth, contains about 78% of ethanol, has irritation and harm to the skin of the hand after long-term use, and has strong pungent smell. However, if the content is reduced, the expected antibacterial effect may not be achieved. Therefore, on the premise of not reducing the antibacterial effect of the no-clean hand sanitizer, reducing the alcohol content of the no-clean hand sanitizer becomes a hot spot problem for researching the no-clean hand sanitizer. Furthermore, it is important that the skin be resistant to microorganisms because some substances on the skin surface are acidic and that the skin of both adults and teenagers has a ph of less than 5. When the hand washing-free liquid is alkaline or neutral, the hand skin can feel tight and dry after washing hands. This is because the leave-in hand cleanser damages some substances on the surface of the skin which are acidic, and the pH of the surface of the skin is increased to directly or indirectly influence the metabolism of keratinocytes, thereby causing the disorders of proliferation and differentiation of keratinocytes, altered skin barrier function, reduced compactness of the stratum corneum and the like.

Disclosure of Invention

The invention aims to provide the five-finger wild peach no-clean hand sanitizer which has lower ethanol content and better antibacterial effect.

The invention further aims to provide a preparation method of the five-finger nectarine hand-washing liquid, which can reduce the ethanol content in the hand-washing liquid and increase the antibacterial effect by adding the active components of the five-finger nectarine into the hand-washing liquid, is simple to operate, has controllable parameters and is suitable for industrial mass production.

The invention solves the technical problems by adopting the following technical scheme.

The invention provides a preparation method of a five-finger nectarine hand-washing-free liquid soap, which comprises the following steps:

s1, preparing a Ficus simplicissima lour alcohol extract: cleaning, drying and grinding roots of Ficus simplicissima lour to obtain Ficus simplicissima lour powder, dissolving the Ficus simplicissima lour powder in ethanol, performing ultrasonic extraction, centrifuging, and discarding precipitate to obtain Ficus simplicissima lour ethanol extract;

s2, mixing the Ficus simplicissima lour ethanol extract with glycerol to obtain the Ficus simplicissima lour hand-washing-free liquid.

The invention provides a five-finger nectarine hand-washing-free liquid soap which is prepared according to the preparation method.

The five-finger wild peach no-clean hand sanitizer and the preparation method thereof have the beneficial effects that:

the invention adopts an 80% ethanol-ultrasonic method to extract the active ingredients of the root of the Ficus simplicissima lour, and the active ingredients are mixed with glycerol to prepare the Ficus simplicissima lour hand-washing liquid. Wherein, the five-finger wild peach is a weak acid natural Chinese herbal medicine substance, and the wild peach is added into the hand-washing-free liquid as an active ingredient, so that the concentration of ethanol in the hand-washing-free liquid can be reduced, the damage of the ethanol to the skin of the hand can be reduced, and the antibacterial effect can be increased. In addition, the five-finger nectarine can also endow the hand-washing-free liquid soap with a certain fragrance so as to improve the use feeling of a user.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a graph showing the ph variation of the wash-free five-finger nectarine hand-washing solutions of examples 1-3 and the ethanol wash-free hand-washing solution of comparative example 1;

FIG. 2 is a diagram of E.coli culture medium with small circular paper sheets containing wash-free Ficus simplicissima hand-wash, ethanol extract of Ficus simplicissima, glycerol and ethanol, respectively;

FIG. 3 is a graph showing E.coli growth in five media containing a wash-free Ficus simplicissima lour hand-wash, a Ficus simplicissima lour alcohol extract, glycerol, an ethanol wash-free hand-wash and pure LB medium, respectively;

FIG. 4 is a graph showing E.coli growth in three media containing Ficus simplicissima lour leave-in hand wash, ficus simplicissima lour alcohol extract and ethanol leave-in hand wash, respectively;

fig. 5 is a flow chart of the preparation of the five-finger nectarine no-wash hand-washing liquid.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.

The five-finger wild peach no-clean hand sanitizer and the preparation method thereof in the embodiment of the invention are specifically described below.

Referring to fig. 5, the preparation method of the five-finger nectarine hand-washing-free liquid soap provided by the embodiment of the invention comprises the following steps:

s1, preparing a Ficus simplicissima lour alcohol extract: cleaning root of Ficus simplicissima lour, oven drying, grinding to obtain Ficus simplicissima lour powder, dissolving the Ficus simplicissima lour powder in ethanol, ultrasonic extracting, centrifuging, and removing precipitate to obtain Ficus simplicissima lour ethanol extractive solution. The five-finger wild peach alcohol extract is a weak acid natural Chinese herbal medicine substance, and the five-finger wild peach alcohol extract serving as an active substance is added into the hand-washing-free liquid, so that the hand-washing-free liquid has a bacteriostatic effect, and does not damage some substances with acidic skin surfaces, so that the skin feel is not greasy and is not tight.

Further, in the preferred embodiment of the present invention, the steps of drying and grinding include: and (3) drying the cleaned root of the Ficus simplicissima lour to constant weight, and then putting the dried root into a crusher for grinding to obtain the Ficus simplicissima lour powder.

Further, in the preferred embodiment of the invention, the mass percentage of the ethanol is 75-85%, and the mass volume ratio of the Ficus simplicissima lour powder to the ethanol is 1:15 to 25 (g/mL).

Further, in the preferred embodiment of the invention, the temperature of ultrasonic extraction is 35-39 ℃ and the extraction time is 50-70 min.

Further, in a preferred embodiment of the present invention, the specific steps of centrifugation include: and (3) placing the mixed solution of the five-finger nectarine powder and the ethanol after ultrasonic extraction into a freezing high-speed centrifuge, and centrifuging for 10-20 min at the temperature of 3-5 ℃ and the speed of 7500-8500 r.

Further, in the preferred embodiment of the present invention, the volume ratio of the Ficus simplicissima lour ethanol extract to the glycerol is 3-7: 1.

further, in the preferred embodiment of the invention, the mass percentage of the ethanol in the five-finger nectarine hand-washing-free liquid soap is 60-70%.

The invention adopts an 80% ethanol-ultrasonic method to extract the active ingredients of the root of the Ficus simplicissima lour, and the active ingredients are mixed with glycerol to prepare the Ficus simplicissima lour hand-washing liquid. Wherein, the five-finger wild peach is a weak acid natural Chinese herbal medicine substance, and the wild peach is added into the hand-washing-free liquid as an active ingredient, so that the concentration of ethanol in the hand-washing-free liquid can be reduced, the damage of the ethanol to the skin of the hand is reduced, and meanwhile, the antibacterial effect is also increased. In addition, the five-finger nectarine can also endow the hand-washing-free liquid soap with a certain fragrance so as to improve the use feeling of a user.

The invention also provides the five-finger nectarine hand-washing-free liquid soap which is prepared according to the preparation method.

The features and capabilities of the present invention are described in further detail below in connection with the examples.

Example 1

The five-finger wild peach no-clean hand sanitizer provided by the embodiment is prepared according to the following method:

(1) Preparing the five-finger wild peach alcohol extract: cleaning root of Ficus simplicissima lour, oven drying to constant weight, and grinding into powder in crusher. Then grinding Ficus simplicissima lour into powder, dissolving 5g into 100mL 80% ethanol, ultrasonic extracting at 37deg.C for 60min, centrifuging at 4deg.C for 15min at 8000r in a refrigerated high-speed centrifuge, and removing precipitate to obtain Ficus simplicissima lour ethanol extract.

(2) The preparation of the five-finger nectarine hand-washing-free liquid soap comprises the following steps: the volume ratio of the five-finger wild peach alcohol extract to the glycerol is 3:1, preparing to obtain the five-finger nectarine hand-washing-free liquid soap. Wherein, in the five-finger wild peach hand-washing-free liquid soap, the content of ethanol is 60 percent.

Example 2

(2) The preparation of the five-finger nectarine hand-washing-free liquid soap comprises the following steps: the volume ratio of the five-finger wild peach alcohol extract to the glycerol is 5:1, preparing to obtain the five-finger nectarine hand-washing-free liquid soap. Wherein, in the five-finger wild peach hand-washing-free liquid soap, the content of ethanol is 66 percent.

Example 3

(2) The preparation of the five-finger nectarine hand-washing-free liquid soap comprises the following steps: the volume ratio of the five-finger wild peach alcohol extract to the glycerol is 7:1, preparing to obtain the five-finger nectarine hand-washing-free liquid soap. Wherein, in the five-finger wild peach hand-washing-free liquid soap, the content of ethanol is 70 percent.

Comparative example 1

The comparative example provides an ethanol no-clean hand sanitizer, wherein the mass percent of ethanol in the ethanol no-clean hand sanitizer is 80%, and the ratio of ethanol to glycerin is 7:1.

test example 1

The test example is a comparison test of the ph value of the five-finger wild peach no-wash hand-washing liquid in examples 1 to 3 and the ethanol no-wash hand-washing liquid in comparative example 1 with time, and specifically comprises the following steps:

the five-finger nectarine no-wash hand-wash solutions of examples 1-3 and the ethanol no-wash hand-wash solution of comparative example 1 were first poured into beakers and numbered (1) - (4), respectively. Wherein, (1) is the ratio of the Ficus simplicissima lour ethanol extract to the glycerol of the example 1 is 3: the ratio of the wash-free hand sanitizer of 1 and the ethanol extract of the Ficus simplicissima lour to the glycerol of the (2) No. example 2 is 5: the ratio of the wash-free hand sanitizer of 1 and the ethanol extract of the Ficus simplicissima lour to the glycerol of the (3) No. 3 is 7: the no-wash hand sanitizer of (1), 80% ethanol to glycerol ratio of (4) comparative example 1, was 7:1. And then testing the pH of the five-finger nectarine no-wash hand sanitizer and the ethanol no-wash hand sanitizer control group with three formulas at 0, 1, 3, 6, 12, 24, 48, 96 and 144h respectively, measuring three times at each time point, taking the time when the pH is measured as an abscissa, taking the average pH value of the measured solution as an ordinate, and drawing a line graph of the change condition of the pH value of the four no-wash hand sanitizers in 6d (144 h) along with time.

Fig. 1 shows a graph of the ph change lines of the five-finger nectarine leave-in hand washes of examples 1-3 and the ethanol leave-in hand wash of comparative example 1. As shown in fig. 1, the PH of the no-clean hand sanitizer containing the Ficus simplicissima lour active ingredient (1) - (3) is acidic, and the PH of the no-clean hand sanitizer containing no Ficus simplicissima lour active ingredient (4) is alkaline. The ph change trend of the four groups of hand sanitizers with different formulas is a descending trend. The ph of the No. 1 and No. 4 hand-washing liquid drops faster than that of the No. 2 and No. 3 hand-washing liquid for 48 hours before, and the pH is respectively reduced by 0.65 (9.84 percent) and 0.67 (8.51 percent); however, in the period of 48-144 hours, the pH of the No. 1 hand-washing liquid is obviously more stable than that of No. 2, 3 and 4 hand-washing liquid. Within 48-96 hours, the ph of the No. 1 hand-washing liquid is only reduced by 0.08 (1.37 percent), and the no-washing liquid is almost indistinguishable from the no-washing liquid before 48 hours; however, the decrease in numbers (2), (3) and (4) was large, and the decrease was 0.20 (3.33%), 0.17 (2.78%), and 0.15 (2.08%) respectively. Within 96 to 144 hours, the numbers (1), (2), (3) and (4) were respectively reduced by 0.08 (1.38%), 0.12 (2.07%), 0.21 (3.53%), and 0.27 (3.83%). It can be seen that the stability of the no-clean hand sanitizer (1) and the no-clean hand sanitizer (2) is better than that of the no-clean hand sanitizer (3) and the no-clean hand sanitizer (4) in the period, wherein the stability of the no-clean hand sanitizer (1) is slightly better than that of the no-clean hand sanitizer (2).

Test example 2

The appearance changes of the five-finger nectarine leave-in hand lotion and the ethanol leave-in hand lotion 6d (144 h) in test example 1 were observed, and the results are shown in table 1.

Table 1 appearance change table of hand-washing-free liquid soap

As can be seen from Table 1, the hand sanitizer No. 1 is well miscible with glycerin, does not delaminate and has no precipitate, while the hand sanitizer No. 2 and the hand sanitizer No. 3 have the precipitate phenomenon, and the solution is not as stable as the hand sanitizer No. 1.

Test example 3

The skin feel of the five-finger nectarine leave-in hand lotion and the ethanol leave-in hand lotion in test example 1 was recorded in this test example, and the results are shown in table 2.

Table 2 sensory evaluation table of leave-in hand sanitizer

As can be seen from Table 2, the hand-washing-free liquid of (1) has no greasy feel and no tight feel after being used, and has good skin feel, the hand-washing-free liquid of (2) and (3) have Xu Youni, and the hand-washing-free liquid of (1) is better than the hand-washing-free liquid of (2) and (3).

From test examples 1 to 3, the no-wash hand sanitizer of the five-finger nectarine (1) comprises the following alcohol extract: the pH of the no-wash hand cleanser with glycerol=3:1 is acidic, and the no-wash hand cleanser is stable, does not delaminate, does not precipitate, has a non-greasy skin feel and a non-tight feel, and is better in comprehensive evaluation.

Test example 4

The test example adopts a round filter paper sheet method to explore the in vitro antibacterial effect of the five-finger nectarine hand-washing liquid, the five-finger nectarine alcohol extract, glycerol and ethanol hand-washing liquid, and comprises the following specific steps:

the prepared sterile small round filter paper sheets with a diameter of 6mm were respectively added to the four beakers of Table 3 and immersed for 2 hours. Then, the monoclonal was picked up and placed in 1ml of LB medium, and cultured with shaking at 37℃for 40min at 180 r. The activated bacteria were inoculated onto a solid medium by a plating method. Then dividing a culture medium into four areas, sequentially attaching small round filter paper sheets soaked with the liquid in the No. 1-4 beaker to different areas, sealing and marking. Culturing the coated flat plates at 37 ℃ for 8 hours, observing, measuring and counting the size of the inhibition zone of the small disc on each flat plate, and comparing the inhibition effect of various hand sanitizers and various components thereof on escherichia coli. The size of the inhibition zone is shown in table 4.

Table 3 combinations of different components of no-wash hand wash in four beakers

As shown in FIG. 2, the culture medium diagram of the Escherichia coli is shown, wherein small circular paper sheets containing the wash-free five-finger nectarine hand-washing liquid, the ethanol extract of the five-finger nectarine, glycerol and ethanol wash-free hand-washing liquid are respectively placed. As can be seen from FIG. 2, the E.coli medium on which the small circular paper sheets were placed was cultured for 8 hours, and a zone of inhibition appeared on the plate.

TABLE 4 antibacterial effect of different ingredients on E.coli

As can be seen by combining Table 3 with Table 4, the No. 1 and No. 2 round filter paper sheets with larger inhibition zones are respectively a five-finger nectarine no-clean hand lotion and a five-finger nectarine alcohol extract, the No. 3 round filter paper sheet only soaked with glycerin has no inhibition zone, and the No. 4 round filter paper sheet soaked with ethanol no-clean hand lotion has inhibition zone, but the inhibition zone is smaller than that of No. 1 and No. 2. The antibacterial effect of the wash-free hand sanitizer with the five-finger wild peach active ingredient and the ethanol extract is best and the antibacterial circle is obvious can be obtained primarily by comparing the sizes of the antibacterial circles; the antibacterial effect of the ethanol hand-washing-free liquid soap is inferior; glycerol has no antibacterial effect.

Test example 5

The test example adopts an ultraviolet-visible spectrophotometry method to explore the in vitro antibacterial effect of the wash-free hand sanitizer of the Ficus simplicissima lour, the alcohol extract of the Ficus simplicissima lour, glycerol and the alcohol wash-free hand sanitizer, and the specific steps are as follows:

four conical flasks of 250mL are taken, 170mL of prepared LB liquid culture medium is respectively placed in the four conical flasks, 30mL of the five-finger nectarine no-clean hand sanitizer, the five-finger nectarine alcohol extract, the glycerol and the ethanol no-clean hand sanitizer are respectively taken and added into the conical flasks of 250mL corresponding to the four conical flasks, and the four conical flasks are fully mixed and vibrated. Another 250mL Erlenmeyer flask was placed in 200mL LB liquid medium as a control. Coli was inoculated into 20mL of the liquid medium and cultured at 37℃for 90min at 180 rpm. Respectively adding 2 mL bacteria liquid into the prepared culture medium added with different antibacterial components, culturing at 37 ℃ at 180rpm, and detecting OD value of Escherichia coli in the culture bottle every 40min. Each set of experiments had three biological replicates and three technical replicates.

OD600 values of escherichia coli and negative control groups of the escherichia coli and the negative control groups are respectively tested and cultured for 0, 100, 140, 160, 180, 200 and 220min in LB culture media added with different antibacterial components, the culture time of the escherichia coli is taken as an abscissa, the average OD600 value of bacterial liquid measured at each time point is taken as an ordinate, and growth curves of the escherichia coli in different culture media are drawn.

As shown in FIG. 3, the growth curves of E.coli in five media respectively containing the five-finger nectarine no-wash hand-washing liquid, the five-finger nectarine alcohol extract, glycerol, ethanol no-wash hand-washing liquid and pure LB medium are shown. Wherein, the numbers 1 to 4 in fig. 3 are respectively culture solutions of the five-finger nectarine hand-washing-free liquid, the five-finger nectarine alcohol extract, glycerol and ethanol hand-washing-free liquid, and the number 5 is a negative control group, namely a culture medium without any antibacterial component. As can be seen from FIG. 3, the differentiation of the E.coli growth period of the negative control group was more evident, and the strain used in the test was normal. 0-100 min is a slow period, and the propagation speed is slower because the escherichia coli is suitable for a new culture environment; 100-170 min is a logarithmic growth phase, at the moment, the colibacillus has proper base number and sufficient nutrient substances, and the colibacillus rapidly grows in the logarithmic growth phase; 180-220 min is a stable period, at this time, not only the nutrient components are remained in the culture solution, but also the accumulated toxic substances of the metabolism of the escherichia coli are continuously increased when the nutrient components are continuously consumed, and the propagation speed of the escherichia coli is gradually reduced due to the influence of factors such as ph reduction and the like, so that the number of dead escherichia coli is increased, the proliferation number and the death number are gradually balanced, and the total number of cells is also stable. The growth curve in FIG. 3 does not evolve to the decay phase of E.coli due to too short a incubation time, but does not affect the study of the antibacterial effect of the present test on the components of the Ficus simplicissima leave-in hand sanitizer.

The glycerol group 3 in FIG. 3 also shows a slower, faster, and more stable period of E.coli growth. However, the low OD600 value in the No. 3 bacterial liquid compared with that in the No. 5 bacterial liquid means that the growth and reproduction ability of E.coli in the No. 3 bacterial liquid are not better than those in the No. 5 control group. The OD600 values of No. 1, no. 2 and No. 4 are all around 0, and little E.coli growth is shown, which indicates that 30mL of three substances added to the culture medium all have strong antibacterial effect. In conclusion, the antibacterial effects of the No. 1, the No. 2 and the No. 4 are far greater than those of the glycerol group and the negative group, and are in strong contrast with the glycerol group and the negative group, but the strong and weak antibacterial effects of the No. 1, the No. 2 and the No. 3 cannot be obtained by the test.

Test example 6

The test example researches the antibacterial effect of the wash-free five-finger nectarine hand-washing liquid, the alcohol extract of the five-finger nectarine and the alcohol wash-free hand-washing liquid after being added into a culture medium so as to explore the antibacterial effect among the three, and specifically comprises the following steps:

adding 0.5mL of the five-finger nectarine washing-free liquid soap, the five-finger nectarine alcohol extract and the ethanol washing-free liquid soap into 30mL of prepared LB culture medium, marking as No. 1, no. 2 and No. 3, transferring 0.3 mL bacterial liquid to culture at 37 ℃, measuring OD600 values of the escherichia coli bacterial liquid and a negative control group of 0, 60, 100, 120, 140, 150 and 160min, measuring three times at each time point, taking the culture time of the escherichia coli as an abscissa, taking the average OD600 value of the bacterial liquid measured at each time point as an ordinate, and drawing a growth curve of the escherichia coli in the three culture mediums.

As shown in FIG. 4, the growth curves of E.coli in three media containing the wash-free five-finger nectarine hand-washing liquid, the ethanol extract of five-finger nectarine and the ethanol wash-free hand-washing liquid respectively are shown. As can be seen from FIG. 4, the growth curve of the E.coli added with the antibacterial components in the bacterial liquid is no longer in accordance with the growth curve of the normal E.coli in FIG. 3, and the antibacterial effect of the three antibacterial components can only be distinguished from the time zone of 60 min-150 min. The OD600 values of the three curves at each time are analyzed, so that the antibacterial effect of the Ficus simplicissima lour ethanol extract is the best, the antibacterial effect of the Ficus simplicissima lour washing-free liquid soap is the inferior, and the antibacterial effect of the ethanol washing-free liquid soap is the worst.

The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the invention is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

Claims

1. The preparation method of the five-finger nectarine hand-washing-free liquid soap is characterized by comprising the following steps of:

s1, preparing a Ficus simplicissima lour alcohol extract: cleaning, drying and grinding roots of Ficus simplicissima lour to obtain Ficus simplicissima lour powder, dissolving the Ficus simplicissima lour powder in ethanol, performing ultrasonic extraction, centrifuging and discarding precipitation to obtain Ficus simplicissima lour ethanol extract, wherein the mass percentage of the ethanol is 75-85%, and the mass volume ratio of the Ficus simplicissima lour powder to the ethanol is 1: 15-25, wherein the ultrasonic extraction temperature is 35-39 ℃, the extraction time is 50-70 min, and the specific steps of centrifugation comprise: placing the mixed solution of the five-finger nectarine powder and the ethanol after ultrasonic extraction into a freezing high-speed centrifuge, and centrifuging for 10-20 min at the temperature of 3-5 ℃ and the speed of 7500-8500 r;

s2, mixing the Ficus simplicissima lour ethanol extract with glycerol to obtain a Ficus simplicissima lour washing-free hand-washing liquid, wherein the volume ratio of the Ficus simplicissima lour ethanol extract to the glycerol is 3:1.

2. the preparation method according to claim 1, wherein in step S1, the specific steps of drying and grinding include: and (3) drying the cleaned root of the Ficus simplicissima lour to constant weight, and then putting the dried root into a crusher for grinding to obtain the Ficus simplicissima lour powder.

3. The preparation method of claim 1, wherein the mass percentage of the ethanol in the five-finger nectarine hand-washing free liquid soap is 60-70%.

4. A wash-free five-finger nectarine hand cleanser, characterized by being prepared by the preparation method of any one of claims 1-3.